TW202119941A - Composition for preventing or alleviating lifestyle diseases - Google Patents

Abstract

The present invention addresses the problem of providing a composition for preventing or alleviating lifestyle diseases by way of a safe substance. The inventors discovered a function that promotes intestinal microbial fermentation in curdlan of 30 [mu]m or smaller in mean particle diameter and arrived at the present invention. The present invention can cause the level of short-chain fatty acids, which are metabolites derived from gut microbiota, to rise significantly and regulate the intestinal environment. In addition, since an improvement of the intestinal environment improves systemic metabolism or immune functions, the present invention also has the effect of preventing lifestyle diseases.

Description

Composition for prevention or improvement of lifestyle-related diseases

The present invention relates to a composition for preventing or ameliorating lifestyle-related diseases, which contains a Cadran thermal gel with an average particle diameter of 30 μm or less of active ingredients.

Curdlan curdlan (of Curdlan) is indigestible polysaccharides by fermentation of a microorganism (Agrobacterium (of Agrobacterium) and Alcaligenes (of Alcaligenes) bacteria and the like), the outer cell produced by heating as with The curdle property was recognized by the food industry as a food additive as a gelling agent, stabilizer, and thickener in 2001. It is a very useful food material and has attracted attention. In addition, since it has been processed into a finely pulverized Caldera thermal gel with a fine average particle size, it has improved water retention and elasticity compared to unprocessed Caldera thermal gel. It is expected to be an excellent product from the viewpoint of food processing characteristics. Food improver (Patent Document 1). In addition, Carderan thermal gel is known to be β-1,3-glucan which is formed by β-(1→3) glycoside bonding and has a simple linear structure. Beta-glucans are polysaccharides composed only of glucose, some of which are water-soluble dietary woven fabrics. According to reports, they exhibit several functionalities depending on their bonding methods. For example, with β-1,3-1,6-bonding as the main structure, β-glucans derived from fungi and seaweed can exert anti-tumor effects; with β-1,3-bonding and β-1 , The repeating structure of 4-bond is the main chain, and β-glucan derived from cereals has been shown to be related to the prevention of lifestyle diseases such as coronary artery disease and diabetes.

In addition, in recent years, it has been discovered that bacterial metabolites and short-chain fatty acids in the intestinal tract exert anti-obesity effects via host receptors (Non-Patent Document 1). In addition, in recent years, whole barley flour rich in β-glucan, one of the dietary weaving dimensions, has improved the production of short-chain fatty acids in the intestines and the accompanying appetite suppression effect, and improved insulin sensitivity. It was confirmed to suppress obesity induced by a high-fat diet (Non-Patent Document 2).

However, it has not been known until now that the Caldera thermal gel with an average particle size of 30 μm or less can prevent or improve lifestyle-related diseases. [Prior technical literature] [Patent Literature]

[Patent Document 1] JP 2006-109730 A [Non-patent literature]

[Non-Patent Document 1] Kimura et al. PNAS, 2011, Kimura et al. Nature Commun, 2013 [Non-Patent Document 2] Miyamoto et al., PLoS One, 2018

[Problems to be solved by the invention]

The subject of the present invention is to provide a composition for the prevention or improvement of lifestyle-related diseases using safe substances. [Means to solve the problem]

The inventors of the present case discovered that the Caldera thermal gel with an average particle size of 30 μm or less has the function of improving the environment in the intestinal tract, and completed the present invention.

The present invention provides: (1) A composition for the prevention or improvement of lifestyle-related diseases, containing as an active ingredient, Calderaine gel with an average particle diameter of 30 μm or less, (2) For the composition of (1), the prevention or improvement of lifestyle diseases is to improve the intestinal environment, (3) For the composition of (1), the prevention or improvement of lifestyle diseases is to improve lipid metabolism, (4) For the composition of (1), the prevention or improvement of lifestyle diseases is to improve immunity, (5) As in the composition of (1), the prevention or improvement of lifestyle diseases is anti-obesity, (6) A food that contains the composition of any one of (1) to (5). [Effects of Invention]

With the present invention, short-chain fatty acids, which are metabolites of bacteria in the intestine, can be significantly increased, and the environment in the intestine can be adjusted. In addition, by improving the intestinal environment, systemic metabolism and immune function are improved, so it also has the effect of preventing lifestyle-related diseases.

The prevention and improvement of lifestyle diseases in the present invention means improving the environment in the intestinal tract, improving lipid metabolism, improving immunity and anti-obesity, etc., especially preferably referring to improving the environment in the intestine.

The improvement of the intestinal environment in the present invention refers to the promotion of microbial fermentation by increasing the probiotics in the intestinal flora, thereby adjusting the intestinal environment. The microbial fermentation promotes the production of short-chain fatty acids, etc., and has the effect of improving sugar and lipid metabolism from the intestinal environment.

The improvement of lipid metabolism in the present invention means the improvement of systemic metabolic functions, such as significantly improving blood glucose level and total cholesterol level.

Improving immunity in the present invention means adjusting the intestinal mucosal immune function by adjusting the intestinal environment, such as controlling the production of intestinal endocrine IgA.

In addition, anti-obesity means inhibiting weight gain.

In the present invention, the distribution of the intestinal flora changes due to the increase of probiotics, which means that it promotes the transfer to the anti-obesity type flora, which can promote the increase in the production of bacterial metabolites and short-chain fatty acids in the intestine, thereby Adjust the environment in the intestinal tract. In addition, by improving the intestinal environment, systemic metabolism and immune function are improved, so it has the effect of preventing lifestyle-related diseases.

The Caldera thermal gel with an average particle size of 30μm or less in the present invention is preferably pulverized to an average particle size of 30μm or less using a jet mill or a refrigerated pulverizer, and to 10-30μm. Better. For example, it can be obtained by using the manufacturing method disclosed in JP 2006-109730 A. The Calderaine thermal gel used as a raw material is not particularly limited, and generally used polysaccharides composed of water-insoluble β-1,3-glucan can be used.

In order to obtain the effects of this case, it is necessary to administer the Caldera thermal gel with an average particle size of 30 μm or less as described in the previous paragraph, or to administer a composition containing it as an active ingredient. The composition of the present invention contains, for example, other materials such as maltitol, sorbitol, starch, etc. as excipients and diluents, and can be used as, for example, intestinal environment improvers, lipid metabolism improvers, immune improvers, anti-obesity agents, etc. Medicament use. The method of administering the composition of the present invention is not particularly limited, and oral administration is preferred. Specifically, it can be oral agents such as tablets, powders, granules, pills, suspensions, emulsions, extracts, decoctions, capsules, syrups, liquids, elixirs, extracts, tinctures, liquid extracts, etc. The type of vote.

There is no particular limitation on the dosage of the Caldera thermal gel with an average particle diameter of 30 μm or less in the present invention, as long as it can exhibit the above-mentioned functions. Although the dosage and frequency of administration when administered to humans are different depending on the form of administration, the age and weight of the person being administered, it is usually a Calderan gel with an average particle size of 30μm or less per day for adults The dosage is 300mg～30g, of which 500mg～15g is better, and 1g～10g is especially good, and it is administered once or even several times a day.

Although there is no special restriction on the interval between the injections, continuous injection is preferred. Generally, it is 1 day to 1 year, preferably 1 week to 3 months. The composition of the present invention can be used not only for humans, but also for animals other than humans (non-human animals).

The composition of the present invention can be used for hobbies, functional foods, nutritional supplements, and health foods by using the same steps as general food manufacturing methods except for the steps of containing the composition and raw materials of the present invention in food. Waiting for food manufacturing. The food obtained by this method is a general food except that it contains the Caldera thermal gel with an average particle diameter of 30 μm or less, and can be eaten by the general ingestion method of the food. The food intake of the food should preferably be 300 mg to 30 g of Catalan thermal gel with an average particle diameter of 30 μm or less, and 500 mg to 15 g is preferred, and 1 g to 10 g is particularly preferred. [Example]

Hereinafter, the present invention will be explained in more detail through examples, but the present invention is not limited to these examples.

The following method verifies the effect of the Caldera thermal gel with an average particle size of 30μm or less. Test Example 1 Feeding method and experimental procedure The test feed is based on high-fat food (D12492; Research Diets Inc.), prepared with cellulose (control group, Fujifilm Wako Pure Chemical Co., Ltd., average particle size 38μm), Calderan Thermal gel (general product) (control group, manufactured by Mitsubishi Corporation Life Sciences, with an average particle size of 104μm) and finely crushed Cadran thermal gel (manufactured by Mitsubishi Corporation Life Sciences, with an average particle size of 24μm) individually at 10% ( w/w) Mixed bait feed. Four-week-old male mice of the C57BL/6J strain were allowed to naturally ingest each test feed for 12 weeks, and the effects of high-fat diet inducing obesity to improve metabolism and inhibit obesity were confirmed (3 groups in total, n=10; Figure 1). While tracking changes in body weight every week, we also investigated changes in metabolic-related visceral weight, various metabolic indicators in the blood, and intestinal flora at the end of the test. The data of statistical analysis are expressed as mean±standard deviation. After single factor variance analysis, it was verified by the Tukey-Kramer method. The statistically significant difference is based on p<0.05. In meta 16S intestinal flora analysis, the Benjamini & Hochberg method ( q- value) evaluation ( q <0.05) was used to correct the false discovery rate (FDR) in the comparison of multiple assays. [Example 1]

Weight change Compared with the control group, the group taking the finely pulverized Calderaan gel showed a tendency to suppress weight gain, and it was confirmed that the pulverized Calderaan gel had a tendency to exhibit an anti-obesity effect (Figure 2). [Example 2]

Tissue weight Compared with the control group, there was a tendency for fat tissue weight to decrease by ingesting finely pulverized Calderaine gel (Figure 3). On the other hand, it is worth noting that compared with the control group, the weight of the cecum showed a significantly higher value in the group taking Calderaine gel, while the weight of the cecum showed a significantly higher value in the group taking Calderaine gel. It is more significant (Figure 3). It can be seen that in the intestinal tract, the ingestion of the finely pulverized Cadran thermal gel promotes the fermentation of microorganisms in the intestinal tract. [Example 3]

Determination of blood biochemical data Compared with the control group, the group taking the finely pulverized Calderaine gel showed a significantly lower blood sugar level (Figure 4). In addition, in terms of total cholesterol in plasma, the group that ingested the finely pulverized Calderaine gel also showed a significantly low value (Figure 4). [Example 4]

Short-chain fatty acids in stool Short-chain fatty acids, which are metabolites of bacteria in the intestine, were measured in feces. As a result, compared with the control group, the group that ingested the finely pulverized Calderaine gel showed significantly higher values of acetic acid, propionic acid, and butyric acid among the short-chain fatty acids in feces (Figure 5). In addition, the production profile of this short-chain fatty acid had a similar trend at the 3rd week of the start of dietary load (Figure 6). [Example 5]

Secreted IgA in stool Firstly, the sIgA concentration in feces was used as an indicator to evaluate the effect of ingestion of micronized Calderaine gel on the intestinal mucosal immune system. As a result, compared to the control group, the sIgA production of the group taking the finely pulverized Carderan thermal gel increased significantly (Figure 7). [Example 6]

Analysis of intestinal flora. Next, in order to compare the environment in the intestine, the meta 16S intestinal flora was analyzed using the next-generation sequencer. First, after investigating the distribution of the flora by principal component analysis, it was confirmed that compared with the control group, the intestinal flora of the group that ingested the finely pulverized Calderaine gel had a different flora (Figure 8; left). Next, the results of the comparison with the classification hierarchy (phylum) confirmed that compared with the control group, the groups that ingested the Calderaine gel and the group that ingested the micro-pulverized Calderaine gel showed Bacteroides The increase of Bacteroidetes and the decrease of Firmicutes and Proteobacteria (Figure 8; right). Due to the large changes in the group that ingested the pulverized Calderaine gel, it was shown that the metabolism of the pulverized Calderaline gel was closely related to the intestinal flora. In addition, it is worth noting that in the group that ingested the finely crushed Calderan thermal gel, Verrucomicrobia and Deferribacteres were characteristically increased (Figure 8; right). Plus, with taxonomic class families (family) compares the results of the intestinal flora, found that the increased Bacteroides door (Bacteroidetes) in the Bacteroides mesh (Bacteroidales) the relative share of the population S24-7, in The group that took the finely pulverized Calderaine gel showed a significantly higher value than the control group (Figure 9). On the other hand, compared with the control group, the streptococcus ( Streptococcaceae ) and Clostridiales ( Clostridiales )_XIII families in the group that ingested the finely crushed Carderan thermal gel were significantly reduced (Figure 9) . On the other hand, in the group in which the finely pulverized Calderaine gel was taken, the Alcaligenaceae (Alcaligenaceae) containing Calderaine gel-producing bacteria showed no significant changes.

According to this verification, the anti-obesity effect can be observed by ingesting the Caldera Gel with an average particle size of 30μm or less, indicating that the Caldera Gel with an average particle size of 30 microns or less may help to improve the intestinal environment. In addition, since the intake of Calderaine gel with an average particle size of 30μm or less significantly improves blood sugar and total cholesterol levels, it is expected to improve systemic metabolic functions. In addition, it has been confirmed that the intake of Calderaine gel with an average particle size of 30μm or less has resulted in an increase in the production of short-chain fatty acids, which are bacterial metabolites in the intestinal tract. It is also expected to improve metabolism from the intestinal environment as a starting point.

In addition, regarding the analysis of the flora in the intestinal tract, it was observed that the distribution of the flora changed with the intake of Calderan gel with an average particle size of 30 μm or less. In fact, by ingesting Calderan gel with an average particle size of 30μm or less, the intestinal flora is transformed into a so-called anti-obesity type intestinal flora. It can be inferred that the ingestion of Calder with an average particle size of 30μm or less Lanre gel has the potential to improve systemic metabolism by improving the flora in the intestines. [Industrial availability]

In summary, by ingesting the Caldera Thermal Gel with an average particle size of 30μm or less, it can be expected to improve the host's intestinal environment. By effectively using Calderaine gel with an average particle diameter of 30 μm or less, it is expected to be used to develop food materials that are beneficial to the prevention of lifestyle-related diseases such as obesity and diabetes.

no.

[Figure 1] Ingestion (indicated by "load" in the figure. The same applies to the following figure.) Caldera thermal gel with an average particle size of 30μm or less (in the figure it is represented by finely crushed Caldera thermal gel. In the figure below The same.) The effect on metabolic function caused by ［Picture 2］Weight change [Figure 3] Organization weight [Figure 4] Blood biochemistry data [Figure 5] Short-chain fatty acids in feces (load 12th week) [Figure 6] Short-chain fatty acids in feces (loading week 3) [Figure 7] Secreted IgA in feces ［Picture 8］Analysis of intestinal flora ［Picture 9］Analysis of intestinal flora

Claims (7)

A composition for the prevention or improvement of lifestyle-related diseases, which contains curdlan with an average particle diameter of 30 μm or less as an active ingredient. Such as the composition for the prevention or improvement of lifestyle-related diseases of claim 1, wherein the prevention or improvement of the lifestyle-related diseases is to improve the intestinal environment. The composition for the prevention or improvement of lifestyle diseases according to claim 1, wherein the prevention or improvement of lifestyle diseases is to improve lipid metabolism. The composition for the prevention or amelioration of lifestyle-related diseases of claim 1, wherein the prevention or amelioration of the lifestyle-related diseases is to improve immunity. The composition for the prevention or improvement of lifestyle-related diseases of claim 1, wherein the prevention or improvement of the lifestyle-related diseases is anti-obesity. A food containing the composition for preventing or improving lifestyle-related diseases according to any one of claims 1 to 5. A method for preventing or ameliorating lifestyle-related diseases of humans or non-human animals, using the composition for the prevention or amelioration of lifestyle-related diseases described in claim 1 by administering or ingesting.

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