TW201909886A - Composition for cosmetics, and cosmetic containing said composition - Google Patents

Abstract

Attention is focused on a cuticle layer occurring on the surface of a camellia leaf, which has not been utilized effectively so far. The cuticle layer can be utilized effectively by using an extract produced by extracting a component constituting the cuticle layer, e.g., a wax, with a fat-soluble solvent. As a result, a composition for cosmetics can be provided, which contains a cuticle layer extract extracted from a camellia leaf with a fat-soluble solvent. Also provided is a cosmetic containing the composition for cosmetics.

Description

Cosmetic composition and cosmetic composition incorporating the composition

The present invention relates to an extract derived from the leaves of camellia. More specifically, it relates to an extract derived from the stratum corneum of the surface of the leaves of Camellia.

Camellia is a long-green broad-leaved tree of the genus Camellia, which belongs to the same species as Spanish sage or Chamo. Its wild species is called Japanese camellia. Among the camellia, Izu Islands and Leeshima (Tokyo), Izu (Shizuoka Prefecture), Fukue Island (Nagasaki Prefecture) of the Goto Islands, and Sado Island (Niigata Prefecture) are well-known.

The seed of camellia contains an oil such as oleic acid, and the camellia oil obtained from the seed is used for hair oil, edible oil, and the like. In addition, it is also used as a raw material for pharmaceuticals, paints, and the like. On the other hand, in the leaves of Camellia, the ash of the fallen leaves is used as a glaze or as a mordant for violet dyeing, but the use other than this is hardly known.

In order to effectively utilize the leaves of this camellia, it has been reported that it produces a fermented tea (hereinafter referred to as "mixed tea") in which the leaves of the third tea and the camellia are mixed in a ratio of 9:1, and the mixed tea can lower the serum triacid. Glyceride and phospholipid concentration (Non-Patent Document 1; hereinafter referred to as "Conventional Example 1").

In addition, in recent years, it has been reported that Japanese camellia has a strong anti-allergic and anti-inflammatory effect, and it has been reported to contain: "Okicamelliaside" which is a ellagic acid glycoside which is a substance which suppresses degranulation, and quercetin which is an antioxidant substance. The glycoside "Camellianoside" and the like. In addition, it is said that the sap of the camellia leaves has an inhibitory effect on gastrointestinal bleeding, and it is widely consumed in Okinawa. The scientific and safety verification of the methanol extract obtained from the leaves is reported and reported (see Non-Patent Document 2). ; hereinafter referred to as "known example 2").

The outer side of the leaf or body stem of the terrestrial plant is covered by a layer formed of a fat-soluble compound impenetrable by water (see Fig. 1(A)). First, the outermost side of the leaf surface has a waxy monolayer (waxy layer). The aforementioned waxy system existing herein exists in various states of a crystalline structure to an amorphous structure.

Next, below the waxy layer, there is a layer formed on the cell wall and containing a large amount of fat-soluble substance, which is called a "striatum layer". The keratinous layer functions to protect the leaves or body stems of terrestrial plants from the external environment such as wind and rain, dryness, ultraviolet rays, and pathogenic bacteria.

Moreover, the aforementioned stratum corneum may be further divided into two layers of an upper layer and a lower layer. Here, the upper layer is composed of a wholly-fat substance and is completely keratinized. On the other hand, the lower layer is a layer mainly composed of keratin, and is not composed of a full-fat substance, and sometimes the carbohydrate fibers of the cell wall may protrude therein. Here, the keratin is a polymer of an unsaturated fatty acid, and the wax (herein referred to as a vegetable wax) is a fatty acid ester (see Non-Patent Documents 3 and 4). [Prior Technical Literature] [Non-Patent Literature]

[Non-Patent Document 1] https://www.pref.nagasaki.jp/e-nourin/nougi/topic/ tsubakikongou/H21-56.pdf [Non-Patent Document 2] Japanese Food Science and Technology Society, Vol. 55, No. 8 No. 379 pages - 383 pages August 2008

[Non-Patent Document 3] https://jspp.org/hiroba/q_and_a/detail.html?id=1969 [Non-Patent Document 4] http://www.biol.tsukuba.ac.jp/~algae/BotanyWEB/ Leaf3.html

[Problems to be solved by the invention]

The mixed tea of the conventional example 1 is prepared by mixing the leaves of the third tea and the leaves of the camellia in a ratio of 9:1, and the hot water extract derived from the tea leaves is excellent in the effect as described above. However, since the leaves of Camellia are used as raw materials for the so-called "tea" beverage, the solvent used for extraction at this time is hot water. Therefore, no one has ever investigated the extraction with a high fat-soluble solvent.

Further, the conventional example 2 relates to a plurality of glycoside having anti-allergic and anti-inflammatory effects contained in Japanese camellia. When a compound having a pharmacological effect is to be extracted from a plant or the like, an organic solvent (hydrophilic organic solvent) compatible with water is used, such as methanol or ethyl acetate. Further, the compound having the pharmacological effect as obtained in Conventional Example 2 is a hydrophilic glycoside contained in an extract extracted from methanol.

Therefore, the methanol extract of the mountain tea leaves is verified for safety. Conventional Example 2 is an excellent invention for verifying the safety of a methanol extract, but it is not considered at all that the leaves of Camellia sinensis are extracted with a solvent having a high fat solubility to obtain an extract.

That is to say, the hydrophilic components contained in the leaves of camellia have been utilized to date, but the composition of the stratum corneum formed on the surface of the leaves, which is also characteristic of mountain tea leaves, has hardly been studied and its use has been used. It is hardly known, so there is a problem that it is impossible to achieve the full and effective use of such natural materials as mountain tea.

The present invention has been made in view of the above-mentioned facts, and has focused on the stratum corneum which is present on the surface of the leaves of camellia which has not been effectively utilized, and which is fat-soluble by the use of components such as waxes constituting the stratum corneum. The extract obtained by extracting the solvent is used for the purpose of fully utilizing the leaves of the camellia. [Means for solving the problem]

The inventors of the present invention have made efforts to carry out research as described above, and have found out that the extract obtained from the stratum corneum of the leaves of camellia (hereinafter referred to as "bean tea wax") has the effect of completing the present invention. .

That is, one aspect of the present invention provides a cosmetic composition comprising a stratum corneum extract obtained by extracting leaves of camellia with a fat-soluble solvent. Here, the camellia is preferably selected from the group consisting of a native species and a horticultural variety thereof.

The above-mentioned native species is preferably selected from the group consisting of Camellia japonica, Camellia japonica, and Camellia japonica var. macrocarpa.

In the case of the aforementioned horticultural variety, it is preferably selected from the group consisting of Camellia japonica 'Kamo-hon-nami', Camellia japonica 'Tarokaja', and Camellia japonica 'Tama-no-ura' , Camellia japonica 'Tama-gasumi' (Yu Xia), Camellia japonica 'Moshio' (algae), Camellia japonica 'Ezonishiki' (Shrimp brocade), Camellia japonica 'Soushiarai' (grass wash), Camellia japonica 'Bokuhan' ), Camellia japonica 'Hishi-karaito' (Ling Tang), and Camellia japonica 'Murage' (Mr. Murakami). Further, the leaves of the camellia are preferably either raw leaves or naturally dried leaves.

The fat-soluble solvent is a hydrocarbon solvent, and is preferably selected from the group consisting of n-hexane, cyclohexane, isohexane, methylcyclohexane, n-pentane, isooctane, squalane, and liquid paraffin. A solvent is more preferably n-hexane. Moreover, it is preferable that the extract of the keratinous layer is concentrated by extracting the extract obtained by extracting the above-mentioned fat-soluble solvent.

Another aspect of the present invention is a cosmetic which is capable of blending the above-mentioned cosmetic composition in a quantitative manner. Here, the cosmetic is preferably any one selected from the group consisting of lotions, lotions, creams, face foams, facial cleansers, gels, hair gels, hair gels, hair creams, and lipsticks.

The predetermined amount is preferably in the range of 0.005 to 5% by weight when the weight of the cosmetic containing the composition is 100%.

Furthermore, another aspect of the present invention provides a food composition comprising a cuticle extract obtained by extracting leaves of camellia with a fat-soluble solvent. Here, the camellia is preferably selected from the group consisting of a native species or a horticultural variety thereof.

The above-mentioned native species, horticultural varieties, and the leaves of the aforementioned camellia are preferably as described above. Further, the above-mentioned fat-soluble solvent is also preferably as described above. The stratum corneum extract is preferably one obtained by extracting an extract obtained by extracting the above-described fat-soluble solvent. [Effects of the Invention]

According to the present invention, an extract containing a fat-soluble component obtained by extracting a natural raw material of mountain tea leaves using a fat-soluble solvent can be effectively utilized. Further, in the case of the fat-soluble solvent, since the water-soluble component is not extracted, the water-soluble component can be further extracted, and the tea leaves can be sufficiently utilized. Further, by blending the cosmetic composition containing the cuticle extract of Japanese camellia thus obtained, a cosmetic having high storage stability and inferior quality of the product can be obtained.

[Formation of the Invention]

Hereinafter, the present invention will be described in more detail. In the present invention, the leaves of Camellia are used as a raw material for the stratum corneum extract (Mountain tea wax). The leaf of the aforementioned camellia which is an extracting raw material may be any one of native native or horticultural varieties in Japan.

The term "primary species" as used herein refers to a plant that can survive in a natural state without relying on cultivation; it means a variety of crops, interspecific hybridization, etc. in a natural state.

Further, the horticultural variety refers to a plant variety which is artificially produced by mating, selection, and the like. If the trait is fixed, its trait will be inherited by the next generation; if it is the first generation (F1) of the hybrid, its trait is limited to one generation and cannot be inherited by the next generation.

The leaf system of Japanese camellia is water-repellent on the surface of the blade as shown in Fig. 1(A). Further, when the back side of the blade is observed by a scanning electron microscope (SEM), a microscope image as shown in Figs. 1 (B) and (C) can be obtained. The scale of Fig. 1(B) is 100 nm, and the scale of Fig. 1(C) is 10 nm. Fig. 1(C) shows a portion where the lower right portion has waxy damage and looks bright.

The cross section of the leaves of Japanese camellia is shown in Figure 2. Here, the upper direction (left side) of the paper surface indicates the front side of the blade, and the lower direction (right side) indicates the back surface of the blade. The structure of the leaves of Japanese camellia is that it is a stratum corneum from the front side of the leaf, and has an upper epidermis below it, a grille structure below it, and a spongy tissue below it, followed by a lower epidermis and a stratum corneum. A vascular bundle in which the blade is arranged in the spongy tissue. Further, air holes are scattered around the back surface of the blade.

Here, the skin has an effect of preventing moisture from evaporating from a place other than the pores. Further, the grid-like structure is a two-layer structure, which is different from other plants in that elongated cells are closely arranged, and most of the photosynthesis is carried out by the leaves. Therefore, the chloroplast is a structure in which a large number of cells are arranged along the cell membrane.

Further, in order to perform gas exchange through the pores, the spongy tissue is formed to be rich in an air gap (cell gap), and the cells are arranged in an irregular arrangement. The spongy tissue also contains chloroplasts. Further, various substances synthesized in the grid structure are fed to a vascular bundle disposed in the sponge-like structure, and are used to maintain a plant body or the like.

Examples of the native species include Japanese camellia, Camellia japonica which is a variant thereof, and Camellia japonica var. macrocarpa. In order to adapt to the variety of the snowy areas such as the islands of Yakushima and Okinawa, the Shikuyama tea is adapted to the variants of the snowy areas such as the sea side of Japan.

As horticultural varieties, Camellia japonica 'Kamo-hon-nami', Camellia japonica 'Tarokaja' (Tarooka), Camellia japonica 'Tama-no-ura' (Jade's), Camellia japonica 'Tama -gasumi' (玉霞), Camellia japonica 'Moshio' (algae), Camellia japonica 'Ezonishiki' (shrimp brocade), Camellia japonica 'Soushiarai' (grass wash), Camellia japonica 'Bokuhan' (bu companion), Camellia japonica ' Hishi-karaito' (Ling Tang), and Camellia japonica 'Murage' (Murata).

As the leaves of such camellia, leaflets obtained by trimming or the like may be used, and any dried leaves of dried leaflets (hereinafter referred to as "dried mountain tea leaves") may be used.

The leaf of the camellia as described above is cut into about 0.1 to 2 mm square using, for example, a pulverizer or a multipurpose cutter. The chopped mountain tea is supplied with a fat-soluble solvent as described later. Blades not used for solvent extraction are stored in the shade.

Hereinafter, a case where dry leaves dried by heating are used will be described as an example. The dried leaves cut into about 0.1 to 2 mm squares are placed in a desired amount, for example, 100 g, and placed in a beaker type container having a capacity of about 2 L, and a desired fat-soluble solvent having a capacity of about 9 times the dry leaf weight is added thereto, for example, about 900 mL of n-hexane was shaken at 40 ° C while being extracted at room temperature for about 2 hours.

After the end of the extraction, the contents of the above vessel were transferred to, for example, a Buchner funnel having a diameter of about 300 mm, and the solid component and the extract were separated by filtration. Thereafter, the obtained extract is concentrated, for example, by a rotary evaporator equipped with a water bath to obtain a cuticle extract (Mountain tea wax). The obtained cuticle extract (bean tea wax) contains a fatty acid ester or the like.

The tea leaf wax obtained by the above method is blended into various cosmetics to be quantitatively blended into skin care water, lotion, cream, face foam, cleansing foam, gel, hair lotion, hair gel, hair cream, and Lipstick and other cosmetics. For example, when the weight of the cosmetic composition containing the cosmetic composition (bean tea wax) is 100%, the content of the cosmetic composition (bean tea wax) in the cosmetic is preferably about 0.01 to about A range of 10% by weight.

The reason why the aforementioned blending amount is set to be about 0.01 to about 10% by weight is as follows. This is because, firstly, if it is less than about 0.01% by weight, quality deterioration due to oxidation of the cosmetic cannot be sufficiently prevented. On the other hand, even if it exceeds about 10% by weight, the effect of preventing deterioration of quality due to oxidation of the cosmetic cannot be further improved, and the feeling of use of the cosmetic is also deteriorated. It is more preferably from about 0.1 to about 1% from the balance between the antioxidant effect and the feeling of use.

The properties of the wax of the mountain tea obtained as described above can be confirmed by an antioxidant test, ultraviolet light absorption measurement or the like. The antioxidant test can be confirmed by, for example, dibutylhydroxytoluene (BHT) as a positive control group, and by comparing the degree of inhibition of squalene oxidation by using an appropriate plant wax. [Examples]

The present invention will be described in more detail below by way of examples, but the invention should not be construed as limited.

(Example 1) Discussion on Antioxidation (1) Experimental Materials and Reagents The leaves of Japanese camellia were harvested in February 2017 in Wudao City, Nagasaki Prefecture, dried at 60-70 ° C, and cut into about 0.15. Mm square.

N-hexane, trichloroacetic acid, ethanol, butanol, and hydrochloric acid were purchased from Mishan Pharmaceutical Industry Co., Ltd. Thiobarbituric acid (hereinafter referred to as "TBA") and squalene are purchased from Nacalai Tesque. The candelilla wax of the control sample was purchased from Wako Pure Chemical Industries Co., Ltd., and the dibutyl hydroxytoluene (hereinafter referred to as "BHT") was purchased from Wako Pure Chemical Industries Co., Ltd.

As the TBA reagent, 0.25 N hydrochloric acid containing 0.375% of thiobarbituric acid, 15% of trichloroacetic acid, and 2% of ethanol was prepared. Further, 100 g of dried mountain tea leaves were taken, 9-fold volume of n-hexane was added, and extraction was carried out at 42 ° C for 2 hours, and filtered using qualitative filter paper No. 131.

The filtrate was placed in a 1,000 mL eggplant type flask, and concentrated under reduced pressure using a rotary evaporator in a water bath at 40 °C. The yield of the "Song Tea Cuticle Extract" (hereinafter referred to as "cutinum extract" or "Mountain tea wax") of the test substance was 2%. The obtained concentrate was used as a "cuticle extract" and used in the following experiment.

(2) Discussion on the antioxidation effect of the stratum corneum extract The antioxidant effect of the stratum corneum extract, candelilla wax or BHT when squalene is heated and oxidized is evaluated. In other words, the horny layer extract, candelilla wax or BHT was dissolved in 1 mL of squalene to form a sample at a concentration shown in the following Table 1, and allowed to stand at 50 ° C for 24 hours. As the control group, 1 mL of squalene was used.

Next, 0.1 mL of each sample was placed in a glass test tube, 1 mL of ethanol and 1 mL of TBA reagent were added, and the mixture was shaken and mixed, and the mixture was heated at 100 ° C for 60 minutes. Thereafter, the test tubes were placed in an ice bath and allowed to cool to room temperature, and 2 mL of butanol was added to each test tube, and the mixture was thoroughly shaken, mixed, and allowed to stand. The supernatant was taken from each tube and the absorbance at 535 nm was measured. The results are shown in Table 1.

As shown in Table 1, the formation of a red reactant due to oxidation of squalene can be inhibited by the stratum corneum extract. The cuticle extract (Mountain tea wax) is also more than 6 times stronger than the candelilla wax at the same 0.05% as the candelilla wax, even in the case of a concentration of 1/10. More than double the effect. Moreover, when it is 0.10%, it shows the same level as BHT. It was confirmed from the above that the stratum corneum extract has a strong antioxidant effect.

(Example 2) Analysis of stratum corneum extract (1) Investigation of anti-ultraviolet action The spectrophotometer was used to confirm whether or not the stratum corneum extract obtained in Example 1 (1) had an ultraviolet absorbing effect. The stratum corneum extract was dissolved in n-hexane to 0.1% (w/v), and the change in absorbance at 260 to 450 nm was confirmed. The results are shown in Fig. 3.

As shown in Fig. 3, in the stratum corneum extract, no peak was observed in the range of 280 to 380 nm, and the ultraviolet absorbing effect could not be confirmed.

It was confirmed from the above that the stratum corneum extract alone had a strong anti-oxidation effect, but no ultraviolet absorption.

(2) Component analysis of stratum corneum extract The stratum corneum extract was subjected to infrared absorption (IR) analysis to obtain the spectrum shown in Fig. 4. From the IR spectrum shown in Fig. 4, it was judged that the stratum corneum extract contained an ester wax.

In order to confirm whether the stratum corneum of the camellia leaves contains fatty acid, 1 g of the mountain tea wax was dissolved in 50 mL of hexane and placed in a separatory funnel, and 50 mL of 0.1 mol/L sodium hydroxide was added to vibrate and stand, and then the water layer was placed. Recycled in a beaker. 50 mL of 0.1 mol/L sodium hydroxide was added to the hexane layer, vibrated, and allowed to stand, and the aqueous layer was recovered. Perform this step 2 times. The recovered aqueous layer was transferred to a separatory funnel and an appropriate amount of 1 mol/L of HCl was added to make the pH acidic. 50 mL of hexane was added thereto, and the mixture was shaken and allowed to stand, and a hexane layer was recovered. The aqueous layer was again transferred back to the separatory funnel, vibrated together with hexane, and allowed to stand, and the oil layer was recovered. This step is performed a total of 2 times. An appropriate amount of sodium sulfate (anhydrous) is added to the recovered oil layer for dehydration. This liquid was filtered through a filter paper (TOYO No. 131), and the solvent was removed under reduced pressure, and the result was weighed to 0.005 g, and the content of the fatty acid was determined to be a small amount. (Production Example) The production examples of the above-mentioned cosmetics containing the mountain tea wax are shown in Tables 2 to 4 below.

As described above, a cosmetic incorporating the cosmetic composition of the present invention can be produced. [Industrial availability]

The invention of the present invention is used in the field of cosmetics.

Fig. 1 is a view showing the surface state of the leaves of Japanese camellia. Fig. 1(A) shows the surface water repellent of Japanese camellia. 1(B) and (C) are scanning electron micrographs showing the structure of the back side of the leaves of Japanese camellia.

Fig. 2 is a schematic view showing a cross section of a blade of Japanese camellia. Fig. 3 is a view showing the results of an experiment for investigating ultraviolet absorption of wax of mountain tea leaves. Fig. 4 is a view showing an IR spectrum of a mountain tea wax.

Claims (6)

A cosmetic composition comprising a stratum corneum extract obtained by extracting leaves of camellia with a fat-soluble solvent. The cosmetic composition according to claim 1, wherein the camellia is any one selected from the group consisting of a native species or a horticultural variety thereof. The cosmetic composition according to claim 2, wherein the aforementioned native species is any one selected from the group consisting of Camellia japonica, Camellia japonica, and Camellia japonica var. macrocarpa. The cosmetic composition according to any one of claims 1 to 3, wherein the horny layer extract is an extract obtained by extracting the fat-soluble solvent. A cosmetic composition, wherein the cosmetic composition of claim 4 is blended in a range of 0.005 to 5% by weight, based on 100% by weight of the cosmetic composition containing the cosmetic composition. A food composition comprising a stratum corneum extract obtained by extracting leaves of camellia with a fat-soluble solvent.