TW201815408A - Orchid leaf extract and product containing extract thereof - Google Patents

Orchid leaf extract and product containing extract thereof Download PDF

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Publication number
TW201815408A
TW201815408A TW105133933A TW105133933A TW201815408A TW 201815408 A TW201815408 A TW 201815408A TW 105133933 A TW105133933 A TW 105133933A TW 105133933 A TW105133933 A TW 105133933A TW 201815408 A TW201815408 A TW 201815408A
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Taiwan
Prior art keywords
extract
orchid
orchid leaf
leaf extract
raw material
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TW105133933A
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Chinese (zh)
Inventor
蔡文杰
蕭郁芸
蕭永振
蕭郁萍
蕭郁韋
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蘭卉生物科技股份有限公司
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Priority to TW105133933A priority Critical patent/TW201815408A/en
Priority to CN201710104065.4A priority patent/CN107961195B/en
Publication of TW201815408A publication Critical patent/TW201815408A/en

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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/02Preparations for care of the skin for chemically bleaching or whitening the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

An orchid leaf extract includes at least one low polarity extract and at least one medium polarity extract. The low polarity extract and the medium polarity extract of orchid leafs are capable of using in anti-oxidation, skin-whitening/brightening, freckle removal and prevention of skin pigmentation. A product contains the orchid leaf extract which is added in a cosmetic raw material or a food raw material to produce a cosmetic product or a food product.

Description

蘭花葉子萃取物及其添加產品    Orchid leaf extract and its added products   

本發明係關於一種蘭花葉子〔orchid leaf〕之萃取物及其添加產品;特別是關於一種蘭花葉子之含各種抗氧化活性〔antioxidant activity〕成分萃取物及其添加之化妝品。 The present invention relates to an extract of orchid leaf and its added products; in particular, it relates to an extract of orchid leaf containing various antioxidant activity ingredients and its added cosmetics.

習用蘭花特定部位萃取物相關化妝品,如中華民國專利申請公開第201420107號之〝白花蝴蝶蘭花瓣的萃取物及其製備方法與用途〞發明專利申請案,其揭示一種白花蝴蝶蘭〔Phalaenopsis Amabilis〕花瓣〔petal〕的萃取物及其製備方法。該白花蝴蝶蘭花瓣的萃取物可被用於促進皮膚美白、提升皮膚的保濕能力以及預防和/或延緩皮膚老化。事實上,前述公開第201420107號亦僅揭示白花蝴蝶蘭花瓣的萃取物及其製備方法之相關技術而已。因此,習用相關蘭花萃取技術仍存在有必要進一步提供利用其它蘭花相關部位,以便進行萃取具護膚及抗氧化效果的萃取物之需求。 Conventional cosmetics related to extracts of specific parts of orchids, such as the patent application for "Plumeria phalaenopsis petal extract and preparation method and use" of the Republic of China Patent Application Publication No. 201420107, which discloses a petal of Phalaenopsis Amabilis [Petal] extract and preparation method thereof. The extract of the white flower phalaenopsis petals can be used to promote skin whitening, improve skin's moisturizing ability, and prevent and / or delay skin aging. In fact, the aforementioned publication No. 201420107 only discloses the related technology of the extract of the white flower phalaenopsis petals and its preparation method. Therefore, the conventional orchid extraction technology still needs to further provide the use of other orchid-related parts in order to extract skin care and anti-oxidant extracts.

另一習用含蘭花萃取物化妝品,如美國專利申請公開第20110002968號之〝Cosmetic or Dermatological Composition Containing an Orchid Extract,and Cosmetic Care Method Using Said Composition〞發明專利申請案,其揭示一種添加蘭花萃取物之化妝品組成物及使用該化妝品組成物處理護膚方法。 Another conventional cosmetic product containing orchid extract, such as US Patent Application Publication No. 20110002968, "Cosmetic or Dermatological Composition Containing an Orchid Extract, and Cosmetic Care Method Using Said Composition" invention patent application, which discloses a cosmetic product with orchid extract added Composition and method for treating skin care using the cosmetic composition.

另一習用含蘭花萃取物化妝品,如美國專利申 請公開第20090258093號之〝Use of a brassocattleya marcella koss orchid extract as an active agent to prevent or delay the appearance of signs of cutaneous aging〞發明專利申請案,其揭示一種以蘭花萃取物作為預防或延緩皮膚老化之活化劑。 Another conventional cosmetic product containing orchid extracts, such as US Patent Application Publication No. 20090258093, “Use of a brassocattleya marcella koss orchid extract as an active agent to prevent or delay the appearance of signs of cutaneous aging” invention patent application, which discloses An orchid extract is used as an activator to prevent or delay skin aging.

另一習用含蘭花萃取物化妝品,如美國專利申請公開第20090238848號之〝Use of an Extract of the Orchid Vanda Coerulea as a Skin Hydrating Agent〞發明專利申請案,其揭示一種以大花萬代蘭〔Vanda Coerulea]萃取物作為皮膚保濕劑。 Another conventional cosmetic product containing orchid extracts, such as US Patent Application Publication No. 20090238848, "Use of an Extract of the Orchid Vanda Coerulea as a Skin Hydrating Agent" invention patent application, which discloses a Vanda Coerulea ] The extract acts as a skin moisturizer.

另一習用含蘭花萃取物化妝品,如美國專利申請公開第20090232758號之〝Brassocattleya marcella koss orchid extract and use thereof as skin depigmentation〞發明專利申請案,其揭示一種以蘭花萃取物作為皮膚除斑之相關用途。 Another conventional cosmetic product containing orchid extract, such as US Patent Application Publication No. 20090232758 "Brassocattleya marcella koss orchid extract and use thereof as skin depigmentation" invention patent application, which discloses a related use of orchid extract as skin depigmentation .

事實上,前述美國專利申請公開第20110002968號、第20090258093號、第20090238848號及第20090232758號皆僅揭示以蘭花萃取物作為各種護膚處理,其並未揭示如何能利用蘭花葉子萃取物達成護膚及抗氧化之目的。因此,習用蘭花萃取技術仍存在有必要進一步提供利用其它蘭花相關部位,以便進行萃取具護膚及抗氧化效果的萃取物之需求。 In fact, the aforementioned U.S. Patent Application Publication Nos. 20110002968, 20090258093, 20090238848, and 20090232758 all disclose only the use of orchid extracts as various skin care treatments, but they do not disclose how to use orchid leaf extracts to achieve skin care and anti Purpose of oxidation. Therefore, the conventional orchid extraction technology still needs to further provide the use of other orchid-related parts in order to extract extracts with skin care and antioxidant effects.

另一習用含蘭花萃取物化妝品,如中華民國專利公告第I423826號之〝含蘭花胚胎素之化妝品〞發明專利,其揭示一種含蘭花胚胎素之化妝品。該化妝品包含至少一化妝品原料及至少一蘭花胚胎素。將該蘭花胚胎素添加於該化妝品原料內,以製成一化妝品組成物。另外,該蘭花胚胎素具有高表現之基因群,其可產生對人體皮膚有益成分。 Another habitual use of orchid extract-containing cosmetics, such as the Republic of China Patent Publication No. I423826, "Cosmetics Containing Orchid Embryotoxin" Invention Patent, discloses a cosmetic product containing orchid embryon. The cosmetic comprises at least one cosmetic raw material and at least one orchid embryonin. The orchid embryonin is added to the cosmetic raw material to make a cosmetic composition. In addition, the orchid embryogen has a highly expressed gene group, which can produce beneficial ingredients for human skin.

事實上,前述中華民國專利申請公告第I423826號亦僅揭示含蘭花胚胎素之化妝品之相關技術而已。因此,習用蘭花胚胎萃取技術仍存在有必要進一步提供利用其它蘭花相關部位,以便進行萃取具護膚及抗氧化效果的萃取物之需求。前述諸專利申請案及專利僅為本發明技術背景之參考及說明目前技術發展狀態而已,其並非用以限制本發明。 In fact, the aforementioned Republic of China Patent Application Publication No. I423826 also only discloses the related technology of cosmetics containing orchid embryonin. Therefore, the conventional orchid embryo extraction technology still needs to further provide the use of other orchid-related parts in order to extract skin care and anti-oxidant extracts. The aforementioned patent applications and patents are only references for the technical background of the present invention and describe the current state of technological development, and are not intended to limit the present invention.

有鑑於此,本發明為了滿足上述需求,其提供一種蘭花葉子萃取物及其添加產品,其中該蘭花葉子萃取物包含至少一低極性萃取物或至少一中極性萃取物,而該低極性萃取物或中極性萃取物可作為抗氧化〔DPPH自由基清除、氫氧基清除及抑制脂質氧化〕、美白、去斑及預防黑色素沉澱等生理活性之應用,以增益蘭花葉子萃取物之應用範圍及經濟價值。 In view of this, the present invention provides an orchid leaf extract and an added product thereof in order to meet the above requirements, wherein the orchid leaf extract comprises at least one low-polarity extract or at least one medium-polarity extract, and the low-polarity extract Or medium polar extracts can be used as antioxidant [DPPH free radical scavenging, hydroxyl scavenging and inhibiting lipid oxidation], whitening, spot removal and prevention of melanin precipitation and other physiological activities to increase the scope and economy of orchid leaf extract value.

本發明較佳實施例之主要目的係提供一種蘭花葉子萃取物及其添加產品,其中該蘭花葉子萃取物包含至少一低極性萃取物或至少一中極性萃取物,而該低極性萃取物或中極性萃取物可作為抗氧化〔DPPH自由基清除、氫氧基清除及抑制脂質氧化〕、美白、去斑及預防黑色素沉澱等生理活性之應用,以達成提升蘭花葉子經濟價值之目的。 The main object of the preferred embodiment of the present invention is to provide an orchid leaf extract and an additive product thereof, wherein the orchid leaf extract comprises at least one low polarity extract or at least one medium polarity extract, and the low polarity extract or The polar extract can be used as an antioxidant [DPPH free radical scavenging, hydroxyl scavenging, and inhibiting lipid oxidation], whitening, spot removing and preventing melanin precipitation and other physiological activities to achieve the purpose of enhancing the economic value of orchid leaves.

為了達成上述目的,本發明較佳實施例之蘭花葉子萃取物包含至少一低極性萃取物或至少一中極性萃取物,而該低極性萃取物或中極性萃取物可作為抗氧化、美白、去斑及預防黑色素沉澱之生理活性之應用。 In order to achieve the above object, the orchid leaf extract of the preferred embodiment of the present invention includes at least one low-polarity extract or at least one medium-polarity extract, and the low-polarity or middle-polarity extract can be used as an antioxidant, whitening, Spot and the application of physiological activity to prevent melanin precipitation.

本發明較佳實施例之該蘭花葉子選自一蝴蝶蘭葉子或一維納斯葉子。 The orchid leaf of the preferred embodiment of the present invention is selected from a phalaenopsis leaf or a Venus leaf.

本發明較佳實施例之該萃取物為一蘭花葉萃 取物水溶液。 The extract of the preferred embodiment of the present invention is an aqueous solution of orchid leaf extract.

為了達成上述目的,本發明較佳實施例之蘭花葉子萃取物添加產品包含:至少一化妝品原料,其具有一第一預定含量;及至少一蘭花葉子萃取物,其具有一第二預定含量,且該蘭花葉子萃取物萃取自一蘭花葉子,將該蘭花葉子萃取物添加於該化妝品原料內,以製成一化妝品組成物;其中該蘭花葉子萃取物包含至少一低極性萃取物或至少一中極性萃取物。 In order to achieve the above object, the orchid leaf extract added product according to a preferred embodiment of the present invention includes: at least one cosmetic raw material having a first predetermined content; and at least one orchid leaf extract having a second predetermined content, and The orchid leaf extract is extracted from an orchid leaf, and the orchid leaf extract is added to the cosmetic raw material to make a cosmetic composition; wherein the orchid leaf extract includes at least one low-polarity extract or at least one medium-polarity Extracts.

本發明較佳實施例之該化妝品原料選自一保養品原料、一彩妝品原料或一香水原料,以製成一保養品、一彩妝品或一香水。 The cosmetic raw material of the preferred embodiment of the present invention is selected from a cosmetic raw material, a cosmetic raw material or a perfume raw material to make a cosmetic, a cosmetic or a perfume.

本發明較佳實施例之該化妝品原料選自一粉末狀原料、一膏狀原料或一液態原料。 The cosmetic material of the preferred embodiment of the present invention is selected from a powder material, a paste material or a liquid material.

本發明另一較佳實施例之蘭花葉子萃取物添加產品包含:至少一食品原料,其具有一第一預定含量;及至少一蘭花葉子萃取物,其具有一第二預定含量,且該蘭花葉子萃取物萃取自一蘭花葉子,將該蘭花葉子萃取物添加於該食品原料內,以製成一食品組成物;其中該至少一低極性萃取物或至少一中極性萃取物。 The orchid leaf extract added product according to another preferred embodiment of the present invention includes: at least one food ingredient having a first predetermined content; and at least one orchid leaf extract having a second predetermined content, and the orchid leaf The extract is extracted from an orchid leaf, and the orchid leaf extract is added to the food material to make a food composition; wherein the at least one low-polarity extract or at least one medium-polarity extract.

本發明較佳實施例之該食品組成物為一飲料、一酒類飲品、一食品、一甜點、一調味醬、一調味粉、一健康食品或一保健用品。 The food composition according to a preferred embodiment of the present invention is a beverage, an alcoholic beverage, a food, a dessert, a seasoning sauce, a seasoning powder, a health food or a health product.

本發明較佳實施例之該蘭花葉子選自蝴蝶蘭之蘭花葉子。 The orchid leaf of the preferred embodiment of the present invention is selected from the orchid leaf of the phalaenopsis.

本發明較佳實施例之該萃取物為一蘭花葉萃 取物水溶液。 The extract of the preferred embodiment of the present invention is an aqueous solution of orchid leaf extract.

1a‧‧‧化妝品原料 1a‧‧‧Cosmetic ingredients

1b‧‧‧食品原料 1b‧‧‧Food ingredients

2‧‧‧蘭花葉子萃取物 2‧‧‧ Orchid Leaf Extract

3a‧‧‧化妝品組成物 3a‧‧‧cosmetic composition

3b‧‧‧食品組成物 3b‧‧‧food composition

100‧‧‧加工設備 100‧‧‧ processing equipment

第1圖:本發明較佳實施例之蘭花葉子萃取方法之流程示意圖。 FIG. 1 is a schematic flowchart of an orchid leaf extraction method according to a preferred embodiment of the present invention.

第2圖:本發明較佳實施例之蘭花葉子萃取物之自由基清除能力測定結果之示意圖。 FIG. 2 is a schematic diagram showing the measurement result of free radical scavenging ability of an orchid leaf extract according to a preferred embodiment of the present invention.

第3圖:本發明較佳實施例之蘭花葉子萃取物之抑制酪胺酸酶活性測定結果之示意圖。 FIG. 3 is a schematic diagram showing the measurement results of tyrosinase inhibitory activity of an orchid leaf extract according to a preferred embodiment of the present invention.

第4圖:本發明較佳實施例之蘭花葉子萃取物之氫氧自由基清除能力測定結果之示意圖。 FIG. 4 is a schematic diagram showing the measurement result of the scavenging ability of oxygen and free radicals of an orchid leaf extract according to a preferred embodiment of the present invention.

第5圖:本發明較佳實施例之蘭花葉子萃取物之脂質氧化抑制能力測定結果之示意圖。 FIG. 5 is a schematic diagram showing the measurement results of the lipid oxidation inhibitory ability of the orchid leaf extract of the preferred embodiment of the present invention.

第6圖:本發明第一較佳實施例之蘭花葉子萃取物添加於產品作業之流程示意圖。 FIG. 6 is a schematic flow chart of adding orchid leaf extract to a product according to the first preferred embodiment of the present invention.

第7圖:本發明第二較佳實施例之蘭花葉子萃取物添加於產品作業之流程示意圖。 FIG. 7 is a schematic flow chart of adding a orchid leaf extract to a product according to a second preferred embodiment of the present invention.

為了充分瞭解本發明,於下文將舉例較佳實施例並配合所附圖式作詳細說明,且其並非用以限定本發明。 In order to fully understand the present invention, the preferred embodiments will be described in detail below with reference to the accompanying drawings, which are not intended to limit the present invention.

本發明較佳實施例之蘭花葉子萃取物、其添加產品及其萃取方法適用於各種蘭花〔例如:蝴蝶蘭、維納斯〔Venus〕或其它蘭花〕及其葉子〔葉部或葉片〕萃取物、其添加產品及其萃取方法,但其並非用以限定本發明之範圍。再者,本發明較佳實施例之蘭花葉子萃取物添加於各種加工品,例如:醫療用產品、藥物、護膚產品或美妝保養品,但其並非用以限定本發明之範圍。 The orchid leaf extract of the preferred embodiments of the present invention, the added product and the extraction method thereof are applicable to various orchids [for example: Phalaenopsis, Venus or other orchids] and their leaves [leaf or leaf] extracts , Its added products and its extraction method, but it is not intended to limit the scope of the invention. In addition, the orchid leaf extract of the preferred embodiment of the present invention is added to various processed products, such as medical products, medicines, skin care products or beauty care products, but it is not intended to limit the scope of the present invention.

一般而言,蘭花葉子通常具有多種抗氧化有效成份〔例如:抑制氫過氧化物〕及多種營養成份〔例如: 降血脂成分〕,因此其萃取物具有高度潛在經濟價值。舉例而言,每株蝴蝶蘭都會長出數張厚肥的闊葉,且其形成交互疊列在基部之上,因此其厚肥的闊葉適用於萃取獲得蝴蝶蘭萃取物。 Generally speaking, orchid leaves usually have multiple anti-oxidant active ingredients [such as: inhibiting hydroperoxide] and various nutritional ingredients [such as hypolipidemic components], so its extracts have high potential economic value. For example, each Phalaenopsis plant grows several thick, broad-leaved leaves, and it forms an overlapping stack on the base. Therefore, its thick, broad-leaved leaves are suitable for extraction to obtain Phalaenopsis extracts.

另外,該蘭花葉子萃取物並沒有特殊異味,且亦不會影響食物外觀〔例如:食物顏色〕的缺點,因此其特別適合添加於各式飲料〔例如:各種茶飲或各種花茶〕、酒類飲品〔例如:蘭花酒〕、食品〔例如:各種麵食或各種糕餅〕、甜點、調味醬、調味粉、健康食品或保健用品。 In addition, the orchid leaf extract has no special odor and does not affect the disadvantages of food appearance (such as food color), so it is particularly suitable for adding to various beverages (such as various tea drinks or various flower teas), alcoholic beverages [For example: orchid wine], food [for example: various pasta or various cakes], desserts, sauces, seasoning powders, health foods or health products.

舉例而言,該蘭花葉子萃取物選擇添加於一乳劑、一凝膠、一軟膏、一乳霜、一面膜〔mask〕、一貼片、一貼布〔pack〕、一塗抹劑、一粉末、一氣溶膠、一噴霧、一乳液、一乳漿、一糊劑、一泡沫劑、一分散液、一滴劑、一慕斯〔mousse〕、一防曬乳〔sun block〕、一防曬油〔suntan lotion〕、一化妝水〔tonic water〕、一粉底〔foundation〕、一眼影〔eye shadow〕、一卸妝產品〔makeup remover product〕、一洗面皂、一香皂〔soap〕或各種清潔產品〔body cleansing product〕。 For example, the orchid leaf extract is selectively added to an emulsion, a gel, an ointment, a cream, a mask, a patch, a pack, an applicator, a powder, An aerosol, a spray, an emulsion, a slurry, a paste, a foam, a dispersion, a drop, a mousse, a sun block, a suntan lotion , A tonic water, a foundation, an eye shadow, a makeup remover product, a facial soap, a soap, or various body cleansing products.

第1圖揭示本發明較佳實施例之蘭花葉子萃取方法之流程示意圖。請參照第1圖所示,本發明較佳實施例之蘭花葉子萃取方法包含步驟S1:首先,提供一預定量之蘭花葉子〔例如:維納斯葉子〕,並將該蘭花葉子於一預定溫度〔例如:-80℃或其它適合低溫處理溫度〕進行清洗及冷凍處理,以獲得一冷凍蘭花葉子。 FIG. 1 illustrates a schematic flowchart of an orchid leaf extraction method according to a preferred embodiment of the present invention. Referring to FIG. 1, a method for extracting orchid leaves according to a preferred embodiment of the present invention includes step S1. First, provide a predetermined amount of orchid leaves (eg, Venus leaves), and place the orchid leaves at a predetermined temperature. [For example: -80 ° C or other suitable low temperature processing temperature] Wash and freeze to obtain a frozen orchid leaf.

請再參照第1圖所示,本發明較佳實施例之蘭花葉子萃取方法包含步驟S2:接著,將該冷凍蘭花葉子進行凍乾處理一預定時間〔例如:48小時或其它適合處理時間〕,以便保留該蘭花葉子之組織活性,以獲得一凍乾蘭 花葉粉。 Please refer to FIG. 1 again, the method for extracting orchid leaves according to the preferred embodiment of the present invention includes step S2: then, freeze-drying the frozen orchid leaves for a predetermined time (eg, 48 hours or other suitable processing time), In order to retain the tissue activity of the orchid leaves, a lyophilized orchid leaf powder is obtained.

請再參照第1圖所示,本發明較佳實施例之蘭花葉子萃取方法包含步驟S3:接著,將該凍乾蘭花葉粉浸泡於一溶液或一溶劑〔例如:乙醇,ethanol〕,以形成一蘭花葉初萃溶液。 Please refer to FIG. 1 again. The orchid leaf extraction method according to the preferred embodiment of the present invention includes step S3. Next, the lyophilized orchid leaf powder is immersed in a solution or a solvent (eg, ethanol) to form An orchid leaf extract solution.

請再參照第1圖所示,本發明較佳實施例之蘭花葉子萃取方法包含步驟S4:接著,將該蘭花葉初萃溶液進行加熱回流〔reflux〕萃取,並利用加入一第一溶劑及一第二溶劑〔例如:乙酸乙酯〔ethyl acetate〕及正己烷〔hexane〕〕進行分配萃取,以獲得至少一中極性萃取物〔medium polarity extract〕及至少一低極性萃取物〔low polarity extract〕。 Please refer to FIG. 1 again. The method for extracting orchid leaves according to the preferred embodiment of the present invention includes step S4. Next, the orchid leaf preliminary extraction solution is subjected to reflux reflux (reflux) extraction, and a first solvent and a The second solvent [such as ethyl acetate and hexane] is subjected to partition extraction to obtain at least one medium polarity extract and at least one low polarity extract.

承上,該蘭花葉子之低極性萃取物或中極性萃取物可作為抗氧化〔DPPH自由基清除、氫氧基清除及抑制脂質氧化〕、美白、去斑及預防黑色素沉澱等生理活性之應用。 In conclusion, the low-polar or medium-polar extracts of the orchid leaves can be used for physiological activities such as antioxidant [DPPH free radical scavenging, hydroxyl scavenging, and inhibition of lipid oxidation], whitening, spot removal, and prevention of melanin precipitation.

請再參照第1圖所示,本發明較佳實施例之蘭花葉子萃取方法包含步驟S5:接著,將該低極性萃取物以減壓濃縮方式或裝置去除溶劑,再利用加入一第三溶劑〔例如:乙酸乙酯〕及一溶液〔例如:水〕進行分配萃取,以獲得一蘭花葉萃取物水溶液。 Please refer to FIG. 1 again. The method for extracting orchid leaves according to the preferred embodiment of the present invention includes step S5. Next, the low-polar extract is concentrated under reduced pressure or using a device to remove the solvent, and a third solvent is added. For example: ethyl acetate] and a solution [for example: water] for partition extraction to obtain an aqueous solution of orchid leaf extract.

第2圖揭示本發明較佳實施例之蘭花葉子萃取物之自由基清除能力測定結果之示意圖。請參照第2圖所示,本發明較佳實施例之蘭花葉子萃取物〔例如:維納斯蝴蝶蘭葉部〕在自由基清除能力測試上,在1mg/mL濃度下〔1%〕具有100%的DPPH自由基清除效果,即表示其帶有抗氧化及自由基清除之能力,因此可預防環境傷害及對抗氧化壓力。 FIG. 2 is a schematic diagram showing a measurement result of a radical scavenging ability of an orchid leaf extract according to a preferred embodiment of the present invention. Please refer to FIG. 2, the orchid leaf extract (for example: Venus Phalaenopsis leaf part) of the preferred embodiment of the present invention has a free radical scavenging capacity test at a concentration of 1 mg / mL [1%] with 100 The DPPH free radical scavenging effect means that it has the ability to scavenge oxidation and free radicals, so it can prevent environmental damage and combat oxidative stress.

事實上,DPPH(1,1-diphenyl-2-picryhydrozyl, C18H12N5O6),為一種穩定的自由基,在波長517nm下具有最高吸收波峰〔λ max〕,當抗氧化劑提供氫原子給DPPH,其於517nm吸收值也會下降。因此,若本發明較佳實施例之蘭花葉子萃取物之吸光值下降的越低,即表示其抗氧化劑清除率越高,其供氫能力也越強。 In fact, DPPH (1,1-diphenyl-2-picryhydrozyl, C 18 H 12 N 5 O 6 ) is a stable free radical with the highest absorption peak [λ max] at a wavelength of 517 nm. When the antioxidant provides hydrogen Atoms give DPPH, and their absorption at 517 nm will also decrease. Therefore, if the absorption value of the orchid leaf extract in the preferred embodiment of the present invention decreases, it means that the higher the antioxidant clearance and the stronger the hydrogen supply capacity.

第3圖揭示本發明較佳實施例之蘭花葉子萃取物之抑制酪胺酸酶活性測定結果之示意圖。請參照第3圖所示,本發明較佳實施例之蘭花葉子萃取物〔例如:維納斯蝴蝶蘭葉子水溶性之萃取物,濃度10%〕在抑制酪胺酸酶活性測試上,以kogic acid作為對照組,其能有效達成美白、去斑、預防黑色素沉澱等功效。 FIG. 3 is a schematic diagram showing the measurement results of tyrosinase inhibitory activity of an orchid leaf extract according to a preferred embodiment of the present invention. Please refer to FIG. 3, the orchid leaf extract of the preferred embodiment of the present invention (for example: water-soluble extract of Venus Phalaenopsis leaves, concentration 10%) is tested on the activity of inhibiting tyrosinase by kogic Acid as a control group, it can effectively achieve whitening, spot removal, prevention of melanin precipitation and other effects.

一般而言,皮膚黑色素形成的主要來源為酪胺酸,在黑色素細胞中,藉由酪胺酸酶催化,使酪胺酸轉變為多巴醌後經氧化作用形成黑色素。藉由多巴醌在波長475nm的吸收值進行判定本發明之蘭花葉子萃取物樣品是否有抑制酪胺酸酶能力。 In general, the main source of skin melanin formation is tyrosine. In melanocytes, tyrosinase is catalyzed to convert tyrosine to dopaquinone and form melanin by oxidation. The absorption value of dopaquinone at a wavelength of 475 nm was used to determine whether the orchid leaf extract sample of the present invention has the ability to inhibit tyrosinase.

第4圖揭示本發明較佳實施例之蘭花葉子萃取物之氫氧自由基清除能力測定結果之示意圖。請參照第4圖所示,本發明較佳實施例之蘭花葉子萃取物在氫氧自由基清除能力測試上,該蘭花葉子〔例如:維納斯蝴蝶蘭葉部〕之低極性萃取物具有可清除過氧化氫〔H2O2〕能力。 FIG. 4 is a schematic diagram showing the measurement results of the hydroxyl radical scavenging ability of an orchid leaf extract according to a preferred embodiment of the present invention. Please refer to FIG. 4. In the test of the ability to scavenge hydrogen and oxygen radicals of the orchid leaf extract of the preferred embodiment of the present invention, the low-polarity extract of the orchid leaf (eg, Venus Phalaenopsis leaf part) has Ability to remove hydrogen peroxide [H 2 O 2 ].

一般而言,過氧化氫活性能力測定為過氧化氫在波長230nm時具有最大吸光值。若過氧化氫與抗氧化維生素C反應時,其吸光值會降低,其顯示吸光值越低表示抗氧化劑捕捉過氧化氫的能力越強;反之,吸光值越高表示抑制過氧化氫生成能力越好。 In general, the hydrogen peroxide activity is determined as the maximum absorbance of hydrogen peroxide at a wavelength of 230 nm. If hydrogen peroxide reacts with antioxidant vitamin C, its light absorption value will decrease. The lower the light absorption value, the stronger the antioxidant's ability to capture hydrogen peroxide. On the contrary, the higher the light absorption value, the more inhibited the hydrogen peroxide production ability. it is good.

第5圖揭示本發明較佳實施例之蘭花葉子萃取物之脂質氧化抑制能力測定結果之示意圖。請參照第5圖所示,本發明較佳實施例之蘭花葉子萃取物在氫氧自由基 清除能力測試上,該蘭花葉子〔例如:維納斯蝴蝶蘭葉部〕之低極性萃取物具有脂質氧化抑制能力,因此其具有抗氧化或預防老化、預防環境傷害、氧化壓力等保護效果。 FIG. 5 is a schematic diagram showing the measurement results of the lipid oxidation inhibitory ability of the orchid leaf extract of the preferred embodiment of the present invention. Please refer to FIG. 5, the orchid leaf extract of the preferred embodiment of the present invention is tested for the ability to scavenge hydrogen and oxygen radicals. The low-polar extract of the orchid leaf (eg, Venus Phalaenopsis leaf part) has lipids. Oxidation inhibiting ability, so it has protective effects such as anti-oxidation or prevention of aging, prevention of environmental damage, and oxidative stress.

一般而言,脂質過氧化測定為不同濃度之試驗樣品分別加入維生素C進行反應,並已對照組維生素C添加脂質,測量紫外光/可見光光譜儀測量波長532nm吸收光值的變化,並評估萃取物對脂質過氧化反應之抑制率。 In general, lipid peroxidation is determined by adding vitamin C to test samples at different concentrations, and adding vitamin C to the control group, and measuring the change in the absorption of light at 532 nm with a UV / visible spectrometer. Inhibition rate of lipid peroxidation.

第6圖揭示本發明第一較佳實施例之蘭花葉子萃取物添加於產品作業之流程示意圖。請參照第6圖所示,本發明第一較佳實施例將一化妝品原料1a及至少一種或數種蘭花葉子萃取物〔或蘭花葉子萃取液或粉末〕2以適當比例於一加工設備100製成一化妝品組成物3a。舉例而言,該化妝品原料1a選自一保養品原料、一彩妝品原料或一香水原料,以製成一保養品、一美妝保養品、一彩妝品或一香水。另外,該化妝品原料1a選自一粉末狀原料、一膏狀原料或一液態原料。該美妝保養品包含肌膚調理用品〔例如:面膜、乳霜或精華液〕。 FIG. 6 is a schematic flow chart of adding orchid leaf extract to a product according to the first preferred embodiment of the present invention. Please refer to FIG. 6. In the first preferred embodiment of the present invention, a cosmetic raw material 1 a and at least one or more orchid leaf extracts [or orchid leaf extracts or powders] 2 are produced in a proper proportion in a processing equipment 100. Into a cosmetic composition 3a. For example, the cosmetic raw material 1a is selected from a cosmetic raw material, a cosmetic raw material or a perfume raw material to make a cosmetic, a cosmetic cosmetic, a cosmetic or a perfume. In addition, the cosmetic raw material 1a is selected from a powder raw material, a paste raw material, or a liquid raw material. This beauty care product contains skin conditioning products (such as a mask, cream or serum).

第7圖揭示本發明第二較佳實施例之蘭花葉子萃取物添加於產品作業之流程示意圖。請參照第7圖所示,相對於第一實施例,本發明第二較佳實施例將一食品原料1b及至少一種或數種蘭花葉子萃取物〔或蘭花葉子萃取液或粉末〕2以適當比例於該加工設備100製成一食品組成物3b。舉例而言,該食品組成物3b選擇為一飲料、一調味醬、一調味粉、一健康食品或一保健用品。本發明另一較佳實施例將至少一種或數種蘭花葉子萃取物〔或蘭花葉子萃取液或粉末〕2添加於各種加工品,其包含一醫療用產品、一藥物或一美妝保養品。 FIG. 7 illustrates a schematic diagram of a process of adding orchid leaf extract to a product according to a second preferred embodiment of the present invention. Referring to FIG. 7, compared to the first embodiment, a second preferred embodiment of the present invention uses a food material 1b and at least one or more orchid leaf extracts [or orchid leaf extracts or powders] 2 to appropriately A food composition 3b is prepared in proportion to the processing equipment 100. For example, the food composition 3b is selected as a beverage, a sauce, a seasoning powder, a health food or a health product. In another preferred embodiment of the present invention, at least one or several orchid leaf extracts [or orchid leaf extracts or powders] 2 are added to various processed products, which include a medical product, a medicine or a beauty care product.

前述較佳實施例僅舉例說明本發明及其技術特徵,該實施例之技術仍可適當進行各種實質等效修飾及/ 或替換方式予以實施;因此,本發明之權利範圍須視後附申請專利範圍所界定之範圍為準。本案著作權限制使用於中華民國專利申請用途。 The foregoing preferred embodiment merely exemplifies the present invention and its technical features, and the technology of this embodiment can still be appropriately implemented with various substantially equivalent modifications and / or replacements; therefore, the scope of the rights of the present invention shall be subject to the attached patent The scope defined by the scope shall prevail. The copyright in this case restricts the use to the patent application of the Republic of China.

Claims (10)

一種蘭花葉子萃取物,其包含一萃取物,其萃取自一蘭花葉子,該萃取物包含至少一低極性萃取物或至少一中極性萃取物,而該低極性萃取物或中極性萃取物可作為抗氧化、美白、去斑及預防黑色素沉澱之生理活性之應用。     An orchid leaf extract comprises an extract extracted from an orchid leaf, the extract comprises at least one low-polarity extract or at least one medium-polarity extract, and the low-polarity or middle-polarity extract can be used as Application of anti-oxidation, whitening, spot-removing and prevention of melanin physiological activity.     依申請專利範圍第1項所述之蘭花葉子萃取物,其中該蘭花葉子選自一蝴蝶蘭葉子或一維納斯葉子。     The orchid leaf extract according to item 1 of the patent application scope, wherein the orchid leaf is selected from a Phalaenopsis leaf or a Venus leaf.     依申請專利範圍第1項所述之蘭花葉子萃取物,其中該萃取物為一蘭花葉萃取物水溶液。     The orchid leaf extract according to item 1 of the patent application scope, wherein the extract is an aqueous solution of orchid leaf extract.     一種蘭花葉子萃取物添加產品,其包含:至少一化妝品原料,其具有一第一預定含量;及至少一蘭花葉子萃取物,其具有一第二預定含量,且該蘭花葉子萃取物萃取自一蘭花葉子,將該蘭花葉子萃取物添加於該化妝品原料內,以製成一化妝品組成物;其中該蘭花葉子萃取物包含至少一低極性萃取物或至少一中極性萃取物。     An orchid leaf extract additive product includes: at least one cosmetic raw material having a first predetermined content; and at least one orchid leaf extract having a second predetermined content, and the orchid leaf extract is extracted from an orchid Leaves, the orchid leaf extract is added to the cosmetic raw material to make a cosmetic composition; wherein the orchid leaf extract comprises at least one low-polarity extract or at least one medium-polarity extract.     依申請專利範圍第4項所述之蘭花葉子萃取物添加產品,其中該化妝品原料選自一保養品原料、一彩妝品原料或一香水原料,以製成一保養品、一彩妝品或一香水。     The orchid leaf extract added product according to item 4 of the scope of the patent application, wherein the cosmetic raw material is selected from a cosmetic raw material, a cosmetic raw material or a perfume raw material to make a cosmetic, a cosmetic or a perfume .     依申請專利範圍第4項所述之蘭花葉子萃取物添加產品,其中該化妝品原料選自一粉末狀原料、一膏狀原料或一液態原料。     The added product of orchid leaf extract according to item 4 of the scope of the patent application, wherein the cosmetic raw material is selected from a powder raw material, a paste raw material or a liquid raw material.     一種蘭花葉子萃取物添加產品,其包含:至少一食品原料,其具有一第一預定含量;及至少一蘭花葉子萃取物,其具有一第二預定含量,且該蘭花葉子萃取物萃取自一蘭花葉子,將該蘭花葉子萃取物添加於該食品原料內,以製成一食品組成物;其中該蘭花葉子萃取物包含至少一低極性萃取物或至少一中極性萃取物。     An orchid leaf extract additive product includes: at least one food ingredient having a first predetermined content; and at least one orchid leaf extract having a second predetermined content, and the orchid leaf extract is extracted from an orchid Leaves, the orchid leaf extract is added to the food material to make a food composition; wherein the orchid leaf extract comprises at least one low-polarity extract or at least one medium-polarity extract.     依申請專利範圍第7項所述之蘭花葉子萃取物添加產 品,其中該食品組成物為一飲料、一酒類飲品、一食品、一甜點、一調味醬、一調味粉、一健康食品或一保健用品。     The orchid leaf extract added product according to item 7 of the scope of the patent application, wherein the food composition is a beverage, a wine drink, a food, a dessert, a sauce, a seasoning powder, a health food or a health care product. Supplies.     依申請專利範圍第4或7項所述之蘭花葉子萃取物添加產品,其中該蘭花葉子選自一蝴蝶蘭葉子或一維納斯葉子。     The orchid leaf extract added product according to item 4 or 7 of the scope of the patent application, wherein the orchid leaf is selected from a phalaenopsis leaf or a Venus leaf.     依申請專利範圍第4或7項所述之蘭花葉子萃取物添加產品,其中該萃取物為一蘭花葉萃取物水溶液。     The orchid leaf extract added product according to item 4 or 7 of the patent application scope, wherein the extract is an aqueous solution of orchid leaf extract.    
TW105133933A 2016-10-20 2016-10-20 Orchid leaf extract and product containing extract thereof TW201815408A (en)

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FR2928549B1 (en) * 2008-03-13 2013-07-26 Lvmh Rech USE OF AN EXTRACT OF BRASSOCATTLEYA MARCELLA KOSS ORCHIDEE AS AN AGENT TO PREVENT OR DELAY THE APPEARANCE OF SIGNS OF SKIN AGING
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