TW201736840A - Method for making an electrochemical test strip - Google Patents

Abstract

A method for making an electrochemical test strip is provided which comprises the steps of sticking a top board on a flow channel board defining at least one flow channel opening and at least one side opening, wherein the at least one side opening is located at the side edge of the electrochemical test strip to be formed, and an electrode substrate, the top board and the flow channel board commonly define at least one flow channel and at least one side groove. As a result, the overflowed glue of a glue layer due to extrusion may be contained in the portion of the at least one side groove on which the glue layer is not coated.

Description

Method for manufacturing electrochemical test piece

The present invention relates to a method for producing an electrochemical test piece, and more particularly to a method for producing an electrochemical test piece which reduces the phenomenon of leakage of adhesive on the side.

Electrochemical Sensor Strips have been used to detect various substances in fluids. The basic principle is to use a reagent to produce one of the analytes in a fluid to be tested. Electrochemically to generate an electrical output signal that is related to the analyte to be measured. When the fluid to be tested is human blood and the analyte is blood sugar, a glucose enzyme and other complexes can be used as a chemical reagent.

Figure 1 shows a schematic view of the appearance of a conventional electrochemical test piece. Figure 2 shows an exploded view of the electrochemical test piece of Figure 1. As shown in FIGS. 1 and 2, the electrochemical test piece 100 is a blood glucose test piece including an electrode substrate 110, a flow path plate 120, and a top plate 130. The electrode substrate 110 includes a circuit layout 112 and a substrate 111, and is formed by printing a circuit layout 112 on a substrate 111 by a printing technique, and the circuit layout 112 has a plurality of electrodes 11a and 11c. The runner plate 120 defines an opening 122 that is formed through the upper surface above the runner plate 120. In order to allow the blood to flow more smoothly, an opening may be provided at the position of the opening 122 of the corresponding flow channel plate 120 of the top plate 130. 135.

3A is a cross-sectional view and a plan view showing an electrode substrate in a step of a method of manufacturing a conventional electrochemical test piece, wherein the cross-sectional view is a cross section taken along line AA in plan view. Fig. 3B is a cross-sectional view and a plan view showing a substrate for a flow path in one step of a method of manufacturing a conventional electrochemical test piece. Fig. 3C is a plan view showing the bonding of the electrode substrate and the substrate for a flow path in one step of the method for producing a conventional electrochemical test piece. Fig. 3D is a cross-sectional view and a plan view showing a substrate for a top plate in one step of a method of manufacturing a conventional electrochemical test piece. As shown in FIGS. 3A to 3D, when the electrochemical test piece 100 is manufactured, the manufacturing method of the electrochemical test piece 100 of the prior art includes the following steps.

As shown in FIG. 3A, step S10: an electrode substrate 110 is provided. The electrode substrate 110 is fabricated by forming at least one circuit layout 112 on a substrate 111.

As shown in FIG. 3B, step S12: providing a first-class track substrate 128, and attaching the flow path plate 120 of the flow path substrate 128 to the electrode substrate 110, wherein the flow path plate 120 defines at least one for forming a flow. An opening 122 of the track 150; and an opening 123 to expose a portion of the circuit layout 112. More specifically, the flow path substrate 128 includes a first-order doping plate 120, an adhesive layer 129, and a release layer 127. The adhesive layer 129 is located between the flow channel substrate 128 and the release layer 127. When the flow channel plate 120 is to be attached to the electrode substrate 110, only the release layer 127 needs to be torn off, and the adhesive layer 129 can be transmitted. The flow channel plate 120 is attached to the electrode substrate 110.

As shown in FIG. 3C, in step S14, after a syringe is positioned over the opening 122 for forming the flow channel 150 by using a drip device, the glucose enzyme is dropped into the flow channel 150 through the syringe, and then dried. Thereby, glucose enzyme is formed on the electrode in the flow channel 150.

As shown in FIG. 3D, in step S16, a top substrate 138 is provided, and the top plate 130 of the top substrate 138 is attached to the flow channel plate 120, so that the flow channel plate 120 is located on the electrode substrate 110 and Between the top plates 130, and the electrode substrate 110, the flow channel plate 120, and the top plate 130 collectively define a first-class track 150. The position of the flow path 150 corresponds to the position of the opening 122 of the flow path plate 120 and has an inlet 125 and an opening 135. More specifically, in a conventional technique, the top substrate 138 includes a top plate 130, an adhesive layer 139, and a release layer 137. The adhesive layer 139 is located between the top substrate 138 and the release layer 137. When the top plate 130 is to be attached to the flow channel plate 120, only the release layer 137 needs to be torn off, and the top plate can be passed through the adhesive layer 139. 130 is attached to the flow channel plate 120.

Finally, the cutting is further performed to form the electrochemical test piece 100 shown in FIG.

When the electrochemical test strip 100 is to be used, the user drops blood to the inlet 125, and the blood enters the flow path 150 from the inlet 125. The blood flows through the flow path 150 due to capillary action, and the gas in the flow path 150 passes from the opening 135. discharge.

It is an object of the present invention to provide a method of manufacturing an electrochemical test strip that reduces leakage of adhesive from the sides. Another object of the present invention is to provide an electrochemical test piece which does not leak adhesive on its side.

According to an embodiment of the invention, a method of fabricating an electrochemical test strip for fabricating an electrochemical test strip comprising the following steps. An electrode substrate is provided, the electrode substrate comprising a circuit layout. Adhering to the electrode substrate of the first-class track substrate of the first-class track substrate, wherein the flow channel plate defines at least a first-class channel opening for forming at least one of the first-class channels; and at least one side opening for the electrification to be formed The position of at least one side of the test piece is learned to form at least one side groove recessed into the at least one side. Applying a second adhesive layer to the substrate for the flow channel, wherein the second adhesive is not coated on at least one side of the opening Floor. Attaching a top plate to the flow channel plate such that the flow channel plate is located between the electrode substrate and the top plate, and the electrode substrate, the flow channel plate and the top plate collectively define the at least first channel and the at least one a side groove, and a portion of the at least one side groove not coated with the second adhesive layer accommodates the second adhesive layer overflowed by pressing.

In one embodiment, the substrate for the flow channel comprises the flow channel plate, a first adhesive layer and a first release layer, wherein the first adhesive layer is located on the side of the flow channel, and the first The release layer is located on the outer side of the first adhesive layer. The step of attaching the top plate to the flow channel plate further comprises: tearing the first release layer, and then bonding the top plate to the flow channel plate through the first adhesive layer.

In one embodiment, in the step of applying a second adhesive layer to the substrate for the flow channel, the second adhesive layer is not coated at all in the opening of the at least one side.

In one embodiment, the flow channel plate further defines at least one circuit opening for exposing a portion of the circuit layout for exposing a portion of the circuit layout.

According to an embodiment of the invention, a method of fabricating an electrochemical test strip for fabricating an electrochemical test strip comprising the following steps. An electrode substrate is provided, the electrode substrate comprising a circuit layout. Adhering to the electrode substrate of the first-class track substrate of the first-class track substrate, wherein the flow channel plate defines at least a first-class channel opening for forming at least one of the first-class channels; and at least one side opening for the electrification to be formed The position of at least one side of the test piece is learned to form at least one side groove recessed into the at least one side. Attaching a top plate to the flow channel plate such that the flow channel plate is located between the electrode substrate and the top plate, and the electrode substrate, the flow channel plate and the top plate collectively define the at least first channel and the at least one Side groove.

In one embodiment, the substrate for the flow channel comprises the flow channel plate and a first adhesive layer. a layer, a second adhesive layer, a first release layer and a second release layer, wherein the first adhesive layer is located on a first side of the flow channel plate, and the first release layer is located at The outer side of the first adhesive layer, the second adhesive layer is located on the second side of the flow channel plate, and the second release layer is located on the outer side of the second adhesive layer. The step of attaching the first-class track plate of the first-class substrate to the electrode substrate further comprises: tearing the first release layer, and then bonding the flow channel plate to the electrode through the first adhesive layer On the substrate. The step of attaching the top plate to the flow channel plate further comprises: tearing the second release layer, and then bonding the top plate to the flow channel plate through the second adhesive layer.

In one embodiment, the flow channel plate further defines at least one circuit opening for exposing a portion of the circuit layout.

According to an embodiment of the invention, an electrochemical test strip comprises an electrode substrate, a first-class track plate and a top plate. The electrode substrate includes a circuit layout. The flow channel plate is adhered to the electrode substrate through a first adhesive layer, and defines a first-purpose channel opening for forming a first-class track, and at least one side opening, wherein the at least one side is located at the electrification The position of at least one side of the test piece is determined to form at least one side groove recessed into the at least one side. Attaching the top plate to the flow channel plate through a second adhesive layer, the flow channel plate is located between the electrode substrate and the top plate, and the electrode substrate, the flow channel plate and the top plate collectively define the At least a first-class track and at least one side groove.

In an embodiment, the at least one side groove receives a portion of the second adhesive layer.

According to an embodiment of the present invention, since the side grooves are formed on both sides of the electrochemical test piece, the excess second adhesive layer can be accommodated, and the residual glue remains on the side of the electrochemical test piece. When the user uses the electrochemical test piece, the side adhesion of the electrochemical test piece does not occur. On the hand, it causes inconvenience in use. In addition, it is also possible to prevent the surface from being uneven due to the partial protrusion of the second adhesive layer, or the displacement of the substrate for the flow path due to the extrusion after the adhesion.

100‧‧‧Electrochemical test piece

110‧‧‧Electrode substrate

111‧‧‧Substrate

112‧‧‧Circuit layout

120‧‧‧flow channel board

122‧‧‧ openings

123‧‧‧ openings

125‧‧‧ entrance

127‧‧‧Fractal layer

128‧‧‧Flower substrate

129‧‧ ‧ adhesive layer

130‧‧‧ top board

135‧‧‧ openings

137‧‧‧Fractal layer

138‧‧‧Top substrate

139‧‧‧Adhesive layer

150‧‧‧ flow path

510‧‧‧Electrode substrate

511‧‧‧Substrate

512‧‧‧Circuit layout

520‧‧‧flow channel board

521‧‧‧ Side opening

522‧‧‧ openings for runners

523‧‧‧Circuit opening

527‧‧‧First release layer

528‧‧‧Flower substrate

529‧‧‧First adhesive layer

530‧‧‧ top board

537‧‧‧Second release layer

539‧‧‧Second adhesive layer

541‧‧‧ side groove

11a, 11c‧‧‧ electrodes

The invention will be more fully understood from the following detailed description and appended claims.

Figure 1 shows a schematic view of the appearance of a conventional electrochemical test piece.

Figure 2 shows an exploded view of the electrochemical test piece of Figure 1.

3A is a cross-sectional view and a plan view showing an electrode substrate in a step of a method of manufacturing a conventional electrochemical test piece, wherein the cross-sectional view is a cross section taken along line AA in plan view.

Fig. 3B is a cross-sectional view and a plan view showing a substrate for a flow path in one step of a method of manufacturing a conventional electrochemical test piece.

Fig. 3C is a plan view showing the bonding of the electrode substrate and the substrate for a flow path in one step of the method for producing a conventional electrochemical test piece.

Fig. 3D is a cross-sectional view and a plan view showing a substrate for a top plate in one step of a method of manufacturing a conventional electrochemical test piece.

4A is a cross-sectional view and a plan view showing an electrode substrate in a step of a method of manufacturing an electrochemical test piece according to an embodiment of the present invention, wherein the cross-sectional view is a cross-sectional view taken along line AA in plan view.

4B is a cross-sectional view and a plan view showing a substrate for a flow path in one step of a method of manufacturing an electrochemical test piece according to an embodiment of the present invention.

4C is a plan view showing a step of bonding the electrode substrate and the substrate for a flow path and applying a second adhesive layer in one step of the method for producing an electrochemical test piece according to an embodiment of the present invention.

4D is a cross-sectional view and a plan view showing a substrate for a top plate in one step of a method of manufacturing an electrochemical test piece according to an embodiment of the present invention.

4E shows an electrochemical test piece formed by cutting a composite panel in accordance with an embodiment of the present invention.

Figure 5 is a cross-sectional view and a plan view showing a substrate for a flow path in one step of a method of manufacturing an electrochemical test piece according to another embodiment of the present invention.

The invention will be described in detail with reference to the drawings in which the same reference numerals will be used to identify the same or similar elements of the plurality of views. It should be noted that the drawings should be viewed in the orientation direction of the labels.

4A is a cross-sectional view and a plan view showing an electrode substrate in a step of a method of manufacturing an electrochemical test piece according to an embodiment of the present invention, wherein the cross-sectional view is a cross-sectional view taken along line AA in plan view. 4B is a cross-sectional view and a plan view showing a substrate for a flow path in one step of a method of manufacturing an electrochemical test piece according to an embodiment of the present invention. Fig. 4C is a plan view showing the bonding of the electrode substrate and the substrate for a flow path in one step of the method for producing an electrochemical test piece according to an embodiment of the present invention. 4D is a cross-sectional view and a plan view showing a substrate for a top plate in one step of a method of manufacturing an electrochemical test piece according to an embodiment of the present invention. 4E shows an electrochemical test piece formed by cutting a composite panel in accordance with an embodiment of the present invention. As shown in FIGS. 4A to 4E, when the electrochemical test piece 500 is manufactured, the method for producing an electrochemical test piece according to the first embodiment of the present invention comprises the following steps.

As shown in FIG. 4A, step S20: an electrode substrate 510 is provided. The electrode substrate 510 is fabricated by forming at least one circuit layout 512 on a substrate 511.

As shown in FIGS. 4B and 4C, step S22: providing a first-class substrate 528 (FIG. 4B), and attaching the flow path plate 520 of the substrate 528 to the electrode substrate 510 (FIG. 4C), wherein the use is further utilized. a cutting technique for cutting at least the first-pass opening 522 for forming the flow path 150 on the entire substrate 528 of the flow path or the flow path plate 520; and at least one opening 521 for the side, located at the electrochemical test piece to be formed A position of at least one side of the 500 to form a notch recessed into the at least one side. In one embodiment, the circuit opening 523 for exposing a portion of the circuit layout 512 is further cut out on the entire substrate 528 for the flow path or on the flow path plate 520. The flow channel substrate 528 includes a first pass plate 520, a first adhesive layer 529, and a first release layer 527. When the flow channel plate 520 is to be attached to the electrode substrate 510, the first release layer 527 of the lowermost side can be torn, and the flow channel plate 520 can be attached to the electrode substrate 510 through the first adhesive layer 529. .

As shown in FIG. 4C, step S24: a second adhesive layer 539 is applied to the flow path substrate 528, wherein the second adhesive layer 539 is not applied to at least a portion of the side opening 521. In one embodiment, the second adhesive layer 539 is only partially applied to at least a portion of the one side opening 521 without filling the entire at least one side opening 521. In one embodiment, the second adhesive layer 539 is formed into a plurality of dot-shaped, square-shaped or irregular-shaped blocks, and is intermittently coated in the side opening 521. In one embodiment, the second adhesive layer 539 is not coated at all in the side opening 521 (as shown in the leftmost electrochemical test piece of FIG. 4C).

As shown in FIG. 4D, step S26: providing a substrate for a top plate, and the substrate for the top plate may be only a top plate 530, and the top plate 530 is adhered to the flow channel plate 520 through the second adhesive layer 539. The flow channel plate 520 is located between the electrode substrate 510 and the top plate 530, and the electrode substrate 510 and the flow channel The plate 520 and the top plate 530 collectively define the first pass 150 and collectively define at least one side groove 541 on the side of the electrochemical test strip 500. The position of the flow path 150 corresponds to the position of the flow path opening 522 of the flow path plate 520, and has an inlet 125 and an opening 135. The position of at least one side groove 541 corresponds to the position of the opening 521 of at least one side of the flow path plate 520.

In this embodiment, since at least one side groove 541 is formed on both sides of the electrochemical test piece 500, the top plate 530 is adhered to the flow path plate 520 through the second adhesive layer 539 in step S26. At the same time, the excess second adhesive layer 539 can be accommodated to avoid the formation of the electrochemical test piece 500, and residual glue remains on the side of the electrochemical test piece 500. When the electrochemical test piece 500 is used, the user does not have the electrochemical test piece 500 adhered to the hand, which causes inconvenience in use. In addition, since the side of the electrochemical test piece 500 of the present invention is formed with at least one side groove 541, the excess second adhesive layer 539 can be accommodated, and the second adhesive layer 539 is prevented from being protruded after being pressed. When the surface is uneven, or the paste is applied, the flow path substrate 528 is displaced.

In addition, in an embodiment, since the second adhesive layer 539 is partially applied to at least a portion of the one side opening 521, at least one of the side openings 521 is not uncoated with the second adhesive layer 539. In part, the excess second adhesive layer 539 can also be accommodated. Further, the portion coated with the second adhesive layer 539 can be appropriately filled in at least one of the side grooves 541 to avoid excessive voids, resulting in a feeling of discomfort on the touch. Further, it is also possible to increase the bonding force between the electrode substrate 510 and the top plate 530, and it is not easy to cause warpage at the side edges.

As shown in FIG. 4E, step S28: cutting the combination plate of the flow path plate 520, the electrode substrate 510, and the top plate 530 in step S26 to form an electrochemical test piece 500.

According to another embodiment of the present invention, a method for manufacturing an electrochemical test piece, as shown in FIG. 5, provides a first-class substrate 528a in step S22, wherein the flow path substrate 528a includes a first-class substrate. The track board 520, the first and second adhesive layers 529 and 539, and the first and second release layers 527 and 537. The first and second adhesive layers 529 and 539 are respectively located on both sides of the flow channel plate 520, and the first and second release layers 527 and 537 are respectively attached to the outer sides of the first and second adhesive layers 529 and 539. Moreover, the flow path substrate 528a defines at least a first-pass opening 522 for forming the flow path 150; and at least one side opening 521 at a position on at least one side of the electrochemical test piece 500a to be formed, A notch recessed into the at least one side is formed. When the flow channel plate 520 is to be attached to the electrode substrate 510, the first release layer 527 of the lowermost side can be torn, and the flow channel plate 520 can be attached to the electrode substrate 510 through the first adhesive layer 529. . And retaining the second release layer 537 on the uppermost side. As shown in FIG. 4D, in step S26, the uppermost release layer 527 of the flow channel substrate 528a is torn, and a top plate 530 is provided, and the top plate 530 is pasted to the flow through the second adhesive layer 539. On the land 520, the flow path plate 520 is placed between the electrode substrate 510 and the top plate 530. According to the embodiment, the second adhesive layer 539 is not disposed on the side of the electrochemical test piece 500a, so that the user does not touch the second adhesive layer 539, and the electrochemical test piece 500a does not adhere to the hand. Inconvenient use.

In summary, in an embodiment, since at least one side groove 541 is formed on both sides of the electrochemical test piece 500, the excess second adhesive layer 539 can be accommodated to avoid the electrochemical test piece. There are residual glue left on the side of 500. When the user uses the electrochemical test piece 500, the side of the electrochemical test piece 500 does not adhere to the hand, which causes inconvenience in use. Further, it is also possible to avoid the occurrence of surface unevenness due to the partial protrusion of the second adhesive layer 539, or the displacement of the substrate 528 for the flow path due to the pressing after the adhesion.

Having described the invention in detail, it will be apparent that the same method can be varied in many ways. Such variations are not to be regarded as a departure from the spirit and scope of the invention. All those obvious modifications to those of ordinary skill in the art are included in the application for this specification. In the range of interest.

520‧‧‧flow channel board

521‧‧‧ Side opening

522‧‧‧ openings for runners

523‧‧‧Circuit opening

527‧‧‧First release layer

528‧‧‧Flower substrate

529‧‧‧First adhesive layer

Claims (9)

An electrochemical test piece manufacturing method for manufacturing an electrochemical test piece, comprising: providing an electrode substrate, the electrode substrate comprising a circuit layout; and bonding a first-class plate of a first-class substrate to the electrode substrate Wherein the flow channel plate defines at least a preferred channel opening for forming at least a first pass; and at least one side opening is located at at least one side of the electrochemical test piece to be formed to form at least At least one side groove recessed in one side; a second adhesive layer is coated on the substrate for the flow channel, wherein the second adhesive layer is not coated on at least a portion of the opening And attaching a top plate to the flow channel plate such that the flow channel plate is located between the electrode substrate and the top plate, and the electrode substrate, the flow channel plate and the top plate collectively define the at least first channel and the At least one side groove, and the portion of the at least one side groove not coated with the second adhesive layer accommodates the second adhesive layer overflowed by pressing. The method for manufacturing an electrochemical test strip according to claim 1, wherein the substrate for the flow channel comprises the flow channel plate, a first adhesive layer and a first release layer, wherein the first adhesive layer Located on the upper side of the flow channel, and the first release layer is located on the outer side of the first adhesive layer, and the step of attaching the top plate to the flow path plate further comprises tearing the first release After the layer, the top plate is attached to the flow channel plate through the first adhesive layer. The method for producing an electrochemical test piece according to claim 1, wherein in the step of applying a second adhesive layer to the substrate for the flow path, the at least one side is not coated at all in the opening. First Two layers of adhesive. The method of fabricating an electrochemical test strip of claim 1, wherein the flow channel plate further defines at least one circuit opening for exposing a portion of the circuit layout for exposing a portion of the circuit layout. An electrochemical test piece manufacturing method for manufacturing an electrochemical test piece, comprising: providing an electrode substrate, the electrode substrate comprising a circuit layout; and bonding a first-class plate of a first-class substrate to the electrode substrate Wherein the flow channel plate defines at least a preferred channel opening for forming at least a first pass; and at least one side opening is located at at least one side of the electrochemical test piece to be formed to form at least At least one side groove recessed in one side; and a top plate is attached to the flow path plate such that the flow path plate is located between the electrode substrate and the top plate, and the electrode substrate and the flow path plate And the top plate collectively defines the at least first pass and the at least one side groove. The method for manufacturing an electrochemical test piece according to claim 5, wherein the substrate for the flow channel comprises the flow channel plate, a first adhesive layer, a second adhesive layer, a first release layer, and a second release layer, wherein the first adhesive layer is located on a first side of the flow channel plate, and the first release layer is located on an outer side of the first adhesive layer, and the second adhesive layer is located The second side of the flow channel plate, and the second release layer is located on the outer side surface of the second adhesive layer, and the step of attaching the first-class road plate of the first-class substrate to the electrode substrate further includes After the first release layer is torn off, the flow channel plate is adhered to the electrode substrate through the first adhesive layer. The step of attaching the top plate to the flow channel plate further comprises: tearing the second release layer, and then bonding the top plate to the flow channel plate through the second adhesive layer. The method of fabricating an electrochemical test strip of claim 6, wherein the flow channel plate further defines at least one circuit opening for exposing a portion of the circuit layout. An electrochemical test piece comprising: an electrode substrate comprising a circuit layout; a first-class track plate adhered to the electrode substrate through a first adhesive layer, and defining a first-class channel opening for forming a first-class track, And at least one side of the opening, wherein the at least one side is located at least one side of the electrochemical test piece for forming at least one side groove recessed into the at least one side; a top plate, wherein the top plate is attached to the flow channel plate through a second adhesive layer, the flow channel plate is located between the electrode substrate and the top plate, and the electrode substrate, the flow channel plate and the top plate are common The at least one of the first lanes and the at least one side groove are defined. The electrochemical test piece of claim 8, wherein the at least one side groove receives a portion of the second adhesive layer.