TW201538173A - Combination, powder and manufacturing method of eels' collagen and ceramide ingredients in body type - Google Patents

Abstract

The present invention is related to powder, which contains collagen and ceramide ingredients in body type, cheaply and efficiently made from inedible parts (mainly, their heads) of eels. The present invention provides a manufacturing method of combination of eel's collagen and ceramide ingredients in body type. The method includes: (1) A step of using a jet of water to clean eels' heads; (2) A step of heating eels' heads in the water and smash them simultaneously; (3) A step of adding enzymes to proceed the enzyme reaction after the temperature cools down stated in Step 2 above; (4) A step of stopping the enzyme reaction through heating further the cooling temperature stated in Step 3 above; (5) A step of separating filtrate and solid particles through reactants obtained from Step 4 above filtered by screening forms; A step of pouring filtrate obtained from Step 5 above into a centrifuge, proceeding separation, and obtaining combination of collagen and ceramide ingredients.

Description

Composition containing powder derived from carp and human body neural steroid component, powder and preparation method thereof

The present invention relates to a collagen derived from salmon and a composition, a powder comprising the human neurosteroid derivative component, and a method for producing the same.

In recent years, among the edible organisms that are not suitable for eating and discarded, the useful compounds that have been explored among the supplements and the like, and attempts to be practical have been attracting attention.

Among them, the head of the squid is inedible as an inedible part and becomes a large amount of waste.

On the other hand, it is known that neuropterin is present in the stratum corneum of the human body and contains moderate moisture, and has a moisturizing function for maintaining skin softness, a function of retaining moisture in the body, and a function of preventing entry of foreign matter from the outside.

Collagen is known as a protein with high moisturizing effect. Residues other than glycine repeated every three residues of the collagen molecule are exposed on the surface of the molecule, and more water molecules can be kept around.

Ceramides have been prepared from cattle. In recent years, there have been problems with BSE (Bovine spongiform encephalopathy) and other studies. For example, a neuropterin or the like derived from a plant has been proposed.

In addition, a method of producing a sphingolipid from a soft part of a discarded pearl or the like at a high yield has been proposed (Patent Document 1).

[First technical literature] [Patent Literature]

Patent Document 1 Japanese Patent No. 3408958

However, the extraction and production of neural guanamine from plants takes time and cost.

In addition, the method for producing a sphingolipid described in Patent Document 1 is to extract one or more soft parts of a species of leeches belonging to any of the gastropods, axils, and cephalopods by an organic solvent, and The method of performing chromatographic analysis and distinguishing the sphingolipid portion by an organic solvent is extremely time consuming.

It is known that collagen has been derived from meat or fish in the past, and the molecular weight of collagen itself is large. Therefore, there has been a question about the effect of moisturizing such as when collagen is contained alone in a supplement or a cosmetic.

It is an object of the present invention to provide a composition or powder comprising collagen and a neuropterin component inexpensively and efficiently from the inedible portion of the squid, primarily the head.

The present invention has the following features.

[1] A method for producing a composition comprising collagen derived from salmon and a human neurosteroid derivative, comprising: (1) a step of washing a head of a squid by a jet of water; (2) heating the head of the squid in water, a step of simultaneously pulverizing; (3) a step of adding an enzyme to carry out an enzymatic reaction after cooling at the temperature of the step (2); (4) further heating from a cooling temperature of the step (3) to stop the enzyme reaction a step of (5) filtering the reactant obtained in the above (4) by a screening program to separate the filtrate from the solid matter; (6) injecting the filtrate obtained in the above (5) into a centrifugal separator and The step of separating and recovering the composition comprising the collagen and the neural guanamine component is carried out.

[2] The method for producing a composition comprising collagen derived from salmon and a human neurosteroid derivative according to [1], characterized in that the total amount of collagen or neuropterin contained is in molecular weight The substance having a molecular weight of 1000 or more in the distribution is 50% or more.

[3] The method for producing a composition comprising collagen derived from squid and a human-type neuropterin component according to [1] or [2], wherein a dextrin is added to the composition, and a spray is used. The mist drying device makes it a powder.

[4] A powder comprising collagen derived from squid and a human neural steroid component, which is produced by using any one of the methods [1] to [3] above.

According to the present invention, a composition and a powder containing a collagen and a neuropterin component can be provided inexpensively and efficiently from the head of the squid.

Fig. 1 is a flow chart showing a method for producing a powder comprising collagen and a ceramide component from salmon according to an embodiment of the present invention.

Figure 2 shows the chemical structure of the neuropterin (the arrow indicates the site of the break in the tandem quality analysis).

Fig. 3 is a graph showing the results of quality analysis of neural guanamine extracted from a sample.

Figure 4 is a graph showing the molecular species composition (content per gram of tissue) of neural guanamine.

Fig. 5 is a graph showing the mass spectrum of the S1 and S2 samples GalCer.

Fig. 6 is a graph showing the molecular species composition of HexCer contained in the S1 and S2 samples.

Fig. 7 is a graph showing the mass spectrum of sphingomyelin contained in the S1 and S2 samples.

Fig. 8 shows the results of sample analysis in the case of containing collagen.

Fig. 9 shows the result of sample analysis in the case where the molecular weight (e.g., 1000 or more) of the collagen and the neuropterin contained in the sample is 50% or more.

Figure 10 is a schematic illustration of the sample analysis method of Figures 9 and 11.

Fig. 11 shows the results of analysis of data samples in the case where the molecular weight (e.g., 1000 or more) of the collagen and the neuropterin contained in the sample is 50% or more.

Hereinafter, embodiments of the present invention will be described in detail.

The composition comprising the collagen and the neuropterin component from the squid having the steps of the present invention is produced according to the following procedure. (1) a step of washing the head of the squid by spraying water; (2) a step of heating the head of the squid in water while pulverizing; (3) after cooling from the temperature of the step (2), adding an enzyme to carry out the enzyme reaction a step of (4) further heating to stop the enzyme reaction from the cooling temperature of the step (3); (5) a step of filtering the reactant obtained in the above (4) by a screening program to separate the filtrate from the solid matter; (6) injecting the filtrate obtained in the above (5) into a centrifugal separator and separating it A step of recovering a composition comprising collagen and a neural guanamine component.

Alternatively, a dextrin may be added to the composition and it may be powdered by a spray drying device to produce a powder comprising collagen and a nerve oxime component.

In the present invention, the squid as a raw material is usually an edible squid, and a species belonging to the genus Anguilla of Anguillidae can be used.

For example, big-tailed squid, European squid, and American squid can be used.

Further, in addition to the above, it is also possible to use a species that does not belong to the above-described species but is generally called squid, such as sputum, sputum, scutellaria, scorpion, scorpion, and the like.

The present invention uses the head of the aforementioned squid.

The present invention efficiently produces a composition comprising a collagen and a neural guanamine component using a previously discarded head.

For example, when cooking a grilled fillet of salmon, the head as an inedible portion is usually discarded.

The aforementioned head is a portion that is removed from the edible body part during cooking, and may of course be a state in which the body is attached to the bone portion or the head. Further, the present invention is not limited thereto, and may include, for example, internal organs, scales, fins, skin, blood, and the like.

(pre-processing of the head)

Hereinafter, a method for producing a composition comprising collagen and a neural guanamine component of the present invention will be described with reference to Fig. 1 .

Further, in order to remove the odor component of the head of the squid, it is preferred to carry out immersion treatment in a salt water (for example, a concentration of about 1 to 10%) prepared in advance, and to dissolve ammonia or trimethylamine in the saline solution.

In the present embodiment, the water jet is sprayed on the head of the squid which has been immersed in the salt water to remove dirt or bacteria adhering to the outer surface of the head of the carp.

The jet water flow refers to a state of water flow that is increased by the pressure of the water flow, so that the water flow is violently sprayed to the head of the catfish.

Next, the washed head of the squid is put into water and a bucket to be heated. The amount of water to be added is preferably, for example, about 2 to 4 times the quality of the head of the fish to be fed.

For example, 800 kg of water is added to 200 kg of the head of the squid and put into a bucket to be heated and pulverized.

The purpose of the above heating and pulverization treatment is to soften the head of the squid to facilitate the enzyme reaction as the next step.

At the stage of the aforementioned introduction into the tub, the head of the squid may be in a fresh state or in a frozen state.

When the head of the squid is heated in water, it can be a usual heating means such as boiling as a heating means.

In the present embodiment, in the case of freezing the head of the squid, if steam heating is performed, it is preferable to suppress the deterioration of the head of the squid by rapid thawing.

When the head of the squid is in a frozen state, the heating temperature and the heating time in this stage are not particularly limited as long as the head of the squid can be thawed.

For example, when it is heated at 80 to 100 ° C for 30 to 120 minutes, the whole is in a thawed state, which is preferable.

Next, the aforementioned head of the squid is pulverized in heated hot water.

As the pulverization means, a commonly used means can be used.

For example, a ball mill, a sand mill, a jet mill, or the like can be used.

By preliminarily pulverizing the raw material in this stage, the reaction time can be shortened in the enzyme reaction of the next step. The pulverization can also be carried out in two stages.

For example, as the first pulverization treatment, coarse pulverization (having a particle diameter of about 1 to 2 mm) is carried out using a general-purpose mixer, and then, as a second pulverization treatment, a dedicated pulverizer (for example, directional pulverizer Co., Ltd.) is used for further pulverization treatment. Fine fine pulverization (particle size of 200 μm or less).

Then, an enzyme is added to the head of the smashed squid, and the enzyme is reacted to dissolve.

The enzyme to be used for the dissolution is not particularly limited as long as it is an enzyme (peptide bond hydrolase) which is generally used for decomposing a protein, and examples thereof include an enzyme containing a protease.

In the present embodiment, the enzyme containing a protease is an enzyme that decomposes an animal protein, and is not particularly limited as long as it is an enzyme containing a protease. Examples of the protease include papain, pineapple enzyme, fig enzyme, endoprotease, and endopeptidase.

In the enzymatic reaction, the temperature of the pulverized head of the squid must be adjusted to a temperature suitable for the type of the enzyme to be used.

For example, at a temperature of 30 ° C to 65 ° C, the temperature is controlled at a temperature suitable for the enzyme to be used. In the state, the specific time (for example, 30 to 90 minutes) is maintained to carry out the dissolution reaction of the head of the carp.

In the enzyme reaction, it is preferred to adjust the pH to an optimum pH. In the present embodiment, for example, it is preferably adjusted to a pH of about 6.

Next, in order to stop the enzyme reaction, the dissolved matter in the tub is heated.

The heating temperature and the heating time can appropriately select the temperature and time at which the enzyme to be used is deactivated. For example, the enzyme is inactivated by maintaining at 90 ° C or higher for 20 to 40 minutes (90 ° C × 30 minutes in this embodiment), and the enzyme is stopped. reaction.

In addition, by holding it at 90 ° C or higher for 20 to 40 minutes, it is also possible to kill the Salmonella contained in the blood of the carp.

Then, the reactant obtained in the enzyme reaction (liquid which is dissolved to be in a viscous state) is filtered by a screening program, and the undissolved solid matter and the filtrate are separated by the aforementioned enzyme reaction. In this step, the separated solid matter is mainly the bone portion of the squid. The undissolved bone portion can also be dried and pulverized in a subsequent step as needed to prepare a calcium raw material.

Next, the lipid is separated from the filtrate to obtain an aqueous solution (composition).

As a method of separating the lipid, for example, a centrifugal separator can be used. Alternatively, the filtrate may be allowed to stand, and the lipid separated in the upper layer and the aqueous solution (composition) precipitated in the lower layer may be separated and recovered according to the specific gravity. The obtained aqueous solution (composition) can be directly stored, concentrated, and dried to prepare a powder or the like.

Next, the aqueous solution (composition) is used to explain a method for producing a powder containing collagen and a neuropterin component from salmon.

The dextrin is added in order to adjust the viscosity of the aqueous solution containing the obtained collagen and the neuropterin component from the squid and prepare the powder. The dextrin is usually used in an amount that is used when the liquid is added to the powder. For example, it is added so that the composition ratio: dextrin = 1:2~3 according to the quality ratio.

Then, by using a spray drying device or the like to make the composition into a powder, the powder containing the collagen and the neuropterin component from the squid of the present embodiment can be obtained.

Example

Next, an embodiment of the present invention will be described in detail using the drawings.

Fig. 1 is a flow chart showing a method for producing a powder containing collagen and a neuropterin component from salmon according to an example of the present invention.

As shown in Fig. 1, first, a total of 1000 kg of a squid head (200 kg) + water (800 kg) in a frozen state was placed in a bucket, and smashed by rotating blades in a hot water of a bucket at 90 ° C for 40 minutes. Fish head.

The pulverization was initially coarsely pulverized at a peripheral speed of 50 m/sec for 10 seconds, and then the pulverization was continued until it became about 200 mesh holes.

Next, the entire pulverized liquid was cooled to 55 ° C, and a protease as a decomposing enzyme was added, and the enzyme reaction was carried out while stirring for 20 minutes.

Then, the lysate was again heated to 90 ° C and maintained for 30 minutes to stop the enzyme reaction.

At this stage, the head of the squid is substantially dissolved and becomes viscous.

Next, the lysate in the viscous state is filtered by a screening program to remove solids such as bones which are not dissolved.

The obtained filtrate was poured into a centrifugal separator, and the lipid was separated to obtain an aqueous solution (composition).

20 kg of dextrin was added to the above aqueous solution (composition) and mixed. Then, it was made into a powder (SD powder) by a spray drying apparatus, and the powder containing the collagen from the head of a squid, and a neuroxamide component was obtained. The amount of powder obtained was about 28 kg.

Next, the presence of the collagen and the neuropterin component derived from the squid obtained in the present invention was verified in the following manner.

The obtained neural crest component (neuroguanamine, monohexosyl-based neuropterin) contained in the obtained sample (powder powdered by a spray drying device) was investigated by the following manner.

Figure 2 is the chemical structure of neural guanamine (arrow indicates the site of the break in tandem quality analysis).

As shown in Fig. 2, the neural guanamine has a structure in which sphingosine (S), a fatty acid (N) or an α-hydroxy fatty acid (A) is condensed.

Among the tissues of animals including human bodies, the former (sphingosine (S)) is the most abundant, and is a neuron (Cer) present in any organ, abbreviated as Cer (NS).

The latter (fatty acid (N) or α-hydroxy fatty acid (A) is also abundantly present in the brain, skin, kidney, etc., abbreviated as Cer (AS).

<Sphingolipid extraction treatment (pretreatment) from sample>

The sample was divided into two portions, and each of 207 mg was extracted and placed in a glass test tube to be S1 and S2.

First, in order to eliminate the unevenness of extraction efficiency for more accurate quantification, internal standard lipids (0.24 nmol of neuropterin Cer (NS) 18:1, 0.12 nmol of glucose-based neuropterin GlcCer (NS) were added to samples S1 and S2. 12:0), the sample was subjected to lipid extraction treatment.

For analysis of sphingolipids, phospholipids were removed by a weak base hydrolysis treatment.

Fig. 3 is a graph showing the results of quality analysis of neural guanamine extracted from a sample.

* Marked peaks indicate unidentified admixtures or substances from the environment, and IS indicates internal standard materials.

Figure 4 is a graph showing the molecular species composition (content per gram of tissue) of neural guanamine.

The horizontal axis represents the sum of the carbon numbers of the sphingosine and the fatty acid constituting the neural guanamine, and the vertical axis represents the average value of the measurements of S1 and S2.

As shown in Fig. 3, if the structure is investigated by mass spectrometry of the neuropterin in S1 and S2, there is a Cer(NS) type mainly composed of human sphingosine, and in terms of quantity, length (carbon number) There are more than 16 and the most length (carbon number) 18 in the human body is also quite large.

As shown in Fig. 2, the neuropterin has a structure in which sphingosine and a fatty acid are condensed. According to the results of MS/MS analysis, The peaks of C32 and C34 and the fatty acid have a chain length of 16 and the sphingosine has a chain length of 16 and 18, respectively. The peaks of C40 and C42 have a chain length of 24 and have a double bond. The chain length of sphingosine is 16 and 18, respectively.

0.24 nmol of Cer(NS) 18:1 which is hardly contained in salmon saponin was added to the sample (IS of Fig. 3), and the composition and content of the neuropterin were calculated (Fig. 4).

From these results, if the trace components of the isomers of the respective molecular species having the same total carbon number but different chain lengths of the sphingosine skeleton are ignored, the approximate ratio of the neurosteroids having the sphingosine skeletons of the chain lengths 16 and 18 It becomes about 3:2.

<Comparison of hexose-based neuropterin of samples S1 and S2>

Next, the composition and content of hexose-based neuropterin (HexCer) were investigated.

The monosaccharide bonded to HexCer is usually glucose or galactose, and the monosaccharide contained can be estimated from the dissolution position of the phase chromatographic analysis in order. In glucosyl-ceramide (GlcCer), GlcCer (NS) is eluted earlier than the added internal standard GlcCer(NS) 12:0, and the galactosyl-ceramide GalCer is identical to the internal standard GlcCer (NS) 12:0 or Dissolve later.

Based on these, both were detected in the S1 and S2 samples HexCer, but GalCer was an absolute majority.

As shown in Figure 2, there is no hydroxyl group present in the fatty acids on the neural amine. (N) of (-OH), and (A) which is bonded to the 2 (α) position.

Fig. 5 is a graph showing the mass spectrum of the S1 and S2 samples GalCer.

The left side of Figure 5 is the mass spectrum of GalCer (NS), and the right side is the mass spectrum of GalCer (AS), which represents the data of the lipids extracted from the samples S1, S2, respectively.

Further, the upper mark on the upper portion of the peak indicates the total carbon number contained in GalCer: the number of double bonds contained in the fatty acid when sphingosine is used as the sphingoid base, and the lower part of the mark indicates the m/z value of the peak.

The * mark indicates the elution position of GalCer (AS), and the peak of GalCer (NS) present is mixed for peak tailing.

As shown in Fig. 5, it was found that HexCer (NS) and HexCer (AS) were simultaneously present in the S1 and S2 samples.

Among the product ion spectra obtained by tandem quality analysis, HexCer is characterized by long-chain side such as C48, C50, and C52 (chain lengths of fatty acids of 24, 26, and 28) of human sphingosine having a chain length of 18 The molecular species composition ratio is higher than that of neural amine.

Fig. 6 is a graph showing the molecular species composition of HexCer contained in the samples S1 and S2.

The horizontal axis represents the total carbon number: the number of double bonds contained in the fatty acid when sphingosine is used as the sphingoid base.

The vertical axis indicates the quality (μg) of the neuropterin moiety contained in the HexCer molecular species per 1 g of the sample (S1, S2).

In Fig. 6, the results of studying the HexCer molecular species composition were carried out by using GlcCer(NS) 12:0 (m/z = 644) which is completely absent before the hydrolysis as an internal standard.

GalCer (NS) is characterized by C46 (the sugar number of the sugar is only 6) and the highest content of C40:1 (mainly comprising sphingosine with a chain length of 16 and fatty acid C24:1). C48:1 (mainly including sphingosine having a chain length of 18 and fatty acid C24:1) having a chain length of only 2 is more (Fig. 7 (a) and Fig. 5 described later).

Further, it is known that the structure of such a GalCer (NS) C48 is the same as that of the usual main component of the human body's GalCer (NS).

It is known that GalCer (AS) is mainly composed of a long-chain molecular species of C44 to C50, and the C46 molecular species having the highest content of C40 as a skeleton in the neuropterin molecular species is the highest.

From the above results, it is known that GalCer has a large number of long-chain molecular species, and the content of the neurosteroids in the human-type back chain is longer than that of the neuropterin itself.

Compared with GalCer, GlcCer is a trace component and has a similar tendency to molecular species distribution.

<Contents of neuropterin and HexCer contained in samples (S1, S2)

Table 1 shows the results of calculating the quality (μg) of the neuropterin contained per 1 g of the sample from the data of Figs. 4 and 6 . It can be seen from Table 1 that it contains about 4 times more HexCer than neuropterin.

<Investigation>

According to the above results, it was found that the powder produced from the head of the squid contained about 6 μg of the neuropterin component per 1 g of the sample.

Fig. 7 is a graph showing the mass spectrum of sphingomyelin contained in a sample, the mark indicating the total carbon number (only 5 larger than the corresponding neuropterin): the double contained in the fatty acid when sphingosine is used as the sphingoid base The number of keys.

The sample also contains more sphingomyelin components than neuropterin and HexCer. Sphingomyelin is difficult to break in the tandem mass spectrometry method used this time. Now, the structural information of the ceramide phospholipid skeleton can not be fully obtained, as shown in the figure. As shown in Fig. 7, it indicates a peak intensity distribution very similar to that of neuropterin, and the peak labeled by C45:1 corresponds to the C40 of neuropterin. If neurochelamide is derived from sphingomyelin, more neuropterin is present in the sample.

The exact ratio of the total sphingosine content of the chain lengths 18 and 16 can be obtained by quantifying the sphingosine obtained by decomposing the sphingolipid.

According to this investigation, it was found that the sphingosine skeleton of the neuropterin in the sample contained about 40% of the human body type having a chain length of 18.

Sphingolipids such as neuropterin have received attention as supplements, and raw materials containing human neurosteroids are lacking.

In recent studies, it has also been reported that non-human neuropterin is digested and absorbed by the intestinal epithelium, but the absorbed non-human sphingoid base is excreted to the luminal side by a specific carrier containing human body neurosteroids. The development of raw materials is more important.

The meaning of the powder of the present invention comprising the neuropterin component from salmon is extremely high in industrial applicability.

<Analysis of sample (powder powdered by spray drying device)>

In addition, the analysis results of the powder powdered by the spray drying device obtained by an external mechanism are shown below.

Figure 8 is the result of inspection by the corpse of the 浜 药剂 Pharmacist.

Based on this test checklist, the results of containing 1960 g of hydroxyproline in a 100 g sample were reported.

Hydroxyproline is the main component of collagen and is responsible for the stability of collagen as well as proline.

Collagen has a helical structure in which a polypeptide having a molecular weight of about 100,000 and a molecular weight of about 100,000 is aggregated into a chain of three chains, which forms a fibrous or film-like structure.

Now, in addition to the difficulty in quantifying a specific protein, the structure of collagen differs depending on the origin, etc., and therefore collagen itself cannot be measured.

On the other hand, the type and number of amino acids constituting collagen are extremely characteristic, and one of its characteristics includes hydroxyproline or hydroxyisoline which is not contained in the 20 essential amino acids constituting a normal protein. Amino acid.

These amino acids are specific amino acids contained only in collagen and only proteins similar to it, in particular hydroxyproline accounts for about 10% of all amino acids in collagen. Therefore, hydroxyproline can be considered as a standard for the amount of collagen.

In the present application, as a result of analysis of the aforementioned sample, the result of containing hydroxyproline was achieved, and therefore, the presence of collagen was confirmed.

In addition, FIG. 9 to FIG. 11 are inspection results of the Japanese Food Analysis Center of the general corporation for the same sample.

Based on this analytical test transcript, it is reported that the samples are differentiated within the molecular weight range to determine each Results from the molecular weight distribution (Figure 9).

That is, as shown in Fig. 10, six predetermined standard solutions were added to the analysis sample, and the dissolution time was measured by high performance liquid chromatography (Fig. 11). From the results, it was found that the sample had a large molecular weight (for example, 1,000 or more) of 57% and a small molecular weight (for example, less than 1,000) of 43%.

The result of FIG. 8 whose molecular weight is large is estimated to be collagen, and the molecular weight is small from the results of FIGS. 3 to 7 and is estimated to be neuropterin.

Therefore, it is known that the powder derived from the carp of the present invention contains collagen and human body type ceramide.

In the powder derived from carp of the present invention, both the same collagen derived from squid and human body ceramide can be used in a supplement or a cosmetic. In the case of using the powder derived from squid of the present invention, collagen and human body type ceramide can be simultaneously taken, which is preferable.

[Industrial availability]

The treatment of the head and bone of the carp is also an important means of waste disposal, and the present invention is also an important technique for effectively utilizing unused resources. The present invention also provides a method for producing a powder containing collagen and a neuropterin component inexpensively and efficiently, and a method for effectively using a powder containing the obtained collagen and a neuropterin component, and the industrial applicability is high. .

Claims (4)

A method for producing a composition comprising collagen derived from squid and a human body ceramide component, comprising: (1) a step of washing a head of a squid by spraying a water stream; (2) heating the head of the squid in water while pulverizing Step; (3) a step of adding an enzyme to carry out an enzymatic reaction after cooling at the temperature of the step (2); (4) a step of stopping the enzymatic reaction by further heating from the cooling temperature of the step (3); a step of filtering the reactant obtained in the above (4) by a screening program to separate the filtrate from the solid matter; (6) injecting the filtrate obtained in the above (5) into a centrifugal separator, separating and recovering A step comprising a composition of collagen and a neural guanamine component. A method for producing a composition comprising a collagen derived from salmon and a human neurosteroid derivative according to the first aspect of the invention, characterized in that the total amount of collagen or neuropterin contained is in molecular weight. The substance having a molecular weight of 1000 or more in the distribution is 50% or more. A method for producing a composition comprising a collagen derived from salmon and a human neurosteroid derivative according to claim 1 or 2, wherein a dextrin is added to the composition, and a spray drying device is used. Make it a powder. A powder comprising a collagen derived from salmon and a human body-type neuropterin component, which is produced by using the production method according to any one of claims 1 to 3.