TW201510502A - Pesticide detection apparatus - Google Patents

Abstract

A pesticide detection apparatus includes a LED light source, a cuvette, a light sensor and a microcontroller. The LED light source generates a light path with a given wavelength. The given wavelength corresponds to at least one pesticide. The cuvette is set on the light path and has a chamber to accommodate a solution containing the pesticide. The cuvette is normally transparent so the light on the light path can pass through. The light sensor is set on the light path to receive the light passing through the cuvette, and generates a sensed signal accordingly. The microcontroller is electrically coupled to the light sensor to receive the sensed signal so as to determine whether a pesticide residual can be found.

Description

Agricultural testing device

The present invention relates to an agricultural testing device; and more particularly to an optical pesticide detecting device utilizing a specific wavelength.

Pesticide residue testing is usually divided into chemical and biochemical methods. The inspection unit of the health unit generally uses the chemical method, which takes 3 to 5 days, which is not only time-consuming but also expensive and bulky. The biochemical method utilizes the color reaction of the protein enzyme, which is usually suitable for rapid detection in the field and production and sales. However, it is still necessary to determine by spectroscopic instruments, and it is only possible to measure whether the total amount of organophosphorus agents exceeds, and it is impossible to know which pesticides and their contents. The fruit and vegetable market is usually tested by biochemical methods, and the fruit and vegetables in the fuzzy range will still be auctioned out. After 3-5 days, the results of the chemical test are used to release the furnace. If it is not qualified, it can only be issued. In addition, in actual operation, the pesticide inspection of the health unit is often from one week to two months after sampling to the published results, and the problematic vegetables have already entered the consumer side from the market. Therefore, neither chemical nor biochemical methods can immediately and effectively confirm pesticide residues, making dangerous fruits and vegetables flood the market and endangering consumers' health.

In order to solve the problem that cannot be quickly detected, a pesticide detector using optical principles has been gradually developed. Typically, the illuminant of the optical pesticide detector uses a tungsten or mercury lamp with a wavelength in the range of about 350 to 700 nm. The conventional optical detector has full light The advantage of the spectral function is the detection of pesticides or other chemical components in a variety of different spectra. However, the price is high, the volume is large, the parts are easy to wear and the operation is complicated, so it has not been popularized.

Therefore, at present, there is a need for a low-cost, simple and practical pesticide testing device, which can be used by various fruit and vegetable distributors and even general consumers to detect residual pesticides in real time, and effectively control the health of themselves and other consumers.

The invention relates to a pesticide detecting device, which can effectively and quickly carry out pesticide interpretation by using a fixed wavelength LED as a light source, and is therefore quite suitable as a special instrument for pesticide detection.

According to an embodiment of the present invention, a pesticide detecting device includes an LED light source, a cuvette, a photo sensor, and a microcontroller. The LED light source is capable of producing a light path of a fixed wavelength, wherein the predetermined wavelength corresponds to at least one pesticide. The cuvette is located on the light path and has a container chamber for containing a solution containing the pesticide. The cuvette is generally transparent, allowing light to pass through the light path. The light sensor is located on the light path and receives light passing through the cuvette to generate a sensing signal. The microcontroller is electrically connected to the photo sensor, and receives the sensing signal and accordingly performs the interpretation of the pesticide residue.

In one embodiment, the microcontroller is electrically coupled to the LED light source to transmit a control signal to the LED light source. The pesticide detecting device may further comprise an analog-to-digital converter (ADC) connected between the microcontroller and the photo sensor, for converting the sensing signal into a digital signal for micro control The controller performs analysis and interpretation.

In one embodiment, a first focusing mirror may be included in the light path between the LED light source and the cuvette to focus light on the cuvette. A second focusing mirror may be included in the light path between the cuvette and the photo sensor to focus light onto the photo sensor.

In one embodiment, the pesticide detecting device may further comprise a data input device, a display screen, a data storage device, etc. to provide convenience in use.

In one embodiment, the LED light source is a constant wavelength light source having a wavelength of 350 to 450 nm.

10‧‧‧Pesticide testing device

11‧‧‧ display screen

12‧‧‧Data input device

13‧‧‧ keyboard

14‧‧‧Switch button

15‧‧‧Power supply hole

21‧‧‧LED light source

22‧‧‧ cuvette

23‧‧‧Light sensor

24‧‧‧Microcontroller

25‧‧‧ Analog Digital Converter

26‧‧‧Data storage device

27‧‧‧Battery

28‧‧‧clock generator

29‧‧‧ buzzer

30‧‧‧Power

31, 32‧‧ ‧ focusing mirror

Fig. 1 is a schematic view showing the appearance of a pesticide detecting device according to an embodiment of the present invention.

2 is a circuit block diagram of a pesticide detecting device according to an embodiment of the present invention.

Fig. 3 is a schematic view showing the light path of the pesticide detecting device according to an embodiment of the present invention.

The present invention is intended to be illustrative of the invention, and is not intended to The scope of the rights is subject to any restrictions.

1 is a schematic view of a pesticide detecting device according to an embodiment of the present invention. The appearance of a pesticide detecting device 10 is generally a hand-held electronic device, which is small in size and convenient to carry and use. The surface of the pesticide detecting device 10 is provided with a display screen 11 and a data input device. 12. The display screen 11 can display information such as instrument operation, setting, detection process, test result, data transmission, etc., for the user to read and use. The data input device 12 may include, for example, a keyboard 13 and a switch button 14. The keyboard 13 can input relevant data and data, and the switch key 14 can switch between various modes or other settings. In one embodiment, the side of the pesticide detecting device 10 is provided with a power supply hole 15 which can be connected to the commercial power by a power line to provide the power required by the pesticide detecting device 10. 1 is only an appearance view of the pesticide detecting device 10 of the present invention, and other similar appearances or configurations are also covered by the present invention as long as the technical contents of the present invention are also used.

2 is a circuit block diagram of a pesticide detecting device according to an embodiment of the present invention. The pesticide detecting device 10 includes a display screen 11, a data input device 12, an LED light source 21, a cuvette 22, a photo sensor 23, a microcontroller 24, an analog-to-digital converter 25, a data storage device 26, a battery 27, and a clock generation device. The device 28 and the buzzer 29. The display screen 11 can be a liquid crystal display (LCD), and is electrically connected to the microcontroller 24 for receiving control signals transmitted by the microcontroller 24 and brightness of the LCD screen. Both the LED light source 21 and the photo sensor 23 are electrically connected to the microcontroller 24. The microcontroller 24 transmits a control signal to the LED light source 21. In one embodiment, the LED light source 21 uses a constant wavelength light source having a wavelength of 350 to 450 nm, for example, a fixed wavelength light source of 412 nm. In one embodiment, the photo sensor 23 can be a photodiode that converts the received optical signal into an analog signal such as a voltage or current. An analog-to-digital converter 25 is provided between the photo sensor 23 and the microcontroller 24 for converting the analog signal generated by the photo sensor 23 into a digital signal that the microcontroller 24 can interpret and calculate. The microcontroller 24 has a corresponding spectrum of various pesticides, and the received digital signal can be compared with the spectrum to determine whether there is pesticide residue. Interpretation. In one embodiment, the data input device 12 is coupled to the microcontroller 24 for inputting relevant data and performing instrument settings. The pesticide detecting device 10 can rely on two power supply modes such as the battery 27 or the connection to the commercial power supply 30. Therefore, the microcontroller 24 is connected to the battery 27 or connected to the commercial power supply 30 through the power supply hole 15 of FIG. In fact, it can also be designed to select a separate power source. The data storage device 26 is coupled to the microcontroller 24, which may be provided with a memory providing data storage function. The clock generator 28 provides a time record for detecting the current time to provide immediate and credible test data. The buzzer 30 is coupled to the microcontroller 24 to sound an attention sound at the appropriate time. For example, when the microcontroller 24 receives the signal from the photo sensor 23 to complete the detection, an audible sound may be emitted to alert the user; or an audible alarm is issued to the user when a pesticide residue is detected. In addition, the pesticide detecting device 10 of the present invention may be provided with a USB connector or a wireless module (not shown), or may additionally provide a data printing function, or further use a communication chip to transmit data to the cloud for data processing and calculation. .

Fig. 3 is a schematic view showing the optical path of the pesticide detecting device 10 of the present invention. The LED light source 21 emits LED light to focus the light onto the cuvette 22 via the focusing mirror 31. The cuvette 22 is generally a transparent or translucent design to allow light to pass through. The cuvette 22 has a container chamber that holds a solution containing the pesticide. After the light passes through the cuvette 22, the other focusing mirror 32 can be used to focus the light on the photo sensor 23 to improve the accuracy of the sensing signal. The light path of the pesticide detecting device of the present invention is on the same axis.

Hereinafter, the detection principle of the present invention will be described by taking a pesticide containing organic phosphorus as an example. Organophosphorus is currently the most widely used pesticide in the world. It is not only used for crop pest control, but also for environmental use, especially for the prevention of dengue vector mosquitoes. Health. Organophosphorus pesticides are easily decomposed in the environment, but have strong neurotoxicity. If they are accidentally sprayed, they may cause poisoning. The basic principle of the pesticide identification of the present invention is to form a component of a cuvette solution using an enzyme protein, and a pesticide-free one is used as a control group (normal agent). Using the reaction of the proteinase in the test solution and the organic phosphorus in the pesticide, when the light passes through the cuvette containing the sample solution (testing agent), the light passing rate is calculated by using the color change, for example, the original light is 100%, if 20% is absorbed by the solution in the cuvette, which means that 80% pass. A normal pesticide without pesticide should have 100% light passing through it. In short, the sensing signal is the result of the comparison of the pesticide-free proteinase solution or the pesticide-containing proteinase solution, thereby judging whether there is a pesticide residue.

Further, the solution to be tested usually contains an enzyme protein, a related auxiliary agent (for example, a coloring agent, a receptor, a buffer, etc.) and a test substance (such as various pesticides). Because the enzyme protein is relatively unstable, this test first replaces the pesticide to be tested with alcohol as a control group, and its measured value is chromaticity, which can be used as an indicator of transmittance. The results are shown in Table 1. The control group of Table 1 shows that with the increase of time, the color difference of the interval time (10 seconds apart) remains stable, that is, the chromaticity increases into equal steps, which shows that the instrument to be tested has considerable stability and can be tested.

Table 2 shows that the first sample used a part of the pesticide containing an aminoformate or an organophosphorus, and the pesticide residue was judged by the AChE colorimetric method (Acetylcholinesterase, AChE). The slope of the measured value represents the activity of the enzyme. The greater the slope, the stronger the enzyme activity. Therefore, the difference between the slope of the sample and the control group can be calculated to determine the inhibition rate of the enzyme inhibition. Inhibition rate = [(slope of the control measurement value - slope of the sample group measurement value) / slope of the control measurement value] × 100%. When the detected AChE inhibition rate is less than the preset threshold (for example, 40%), it is judged that the pesticide is within the qualified range, and the instrument can be illuminated by a green light or a blue light to indicate that the test passes. If the AChE inhibition rate is higher than the critical value, the pesticide will exceed the standard and the instrument will light red to warn. The inhibition rate of the first sample shown in Table 2 was about 78.8%, which was significantly higher than the critical value set by 40% in the present example, so that pesticide residues were discriminated.

Table 3 is a second sample example in which the inhibition rate of the sample was detected to be about 80%, and the pesticide residue was also judged.

The pesticide detecting device of the invention is matched with the specific wavelength corresponding to the pesticide, and the determination of whether or not the pesticide residue is effective can be effectively performed by using a simple constant-wavelength LED light source, which is not only high in accuracy, small in size, convenient to carry, and inexpensive. Further, the pesticide detecting device of the present invention has a low component loss rate, and can be simply set as required.

The above is only the detailed description and drawings of the specific embodiments of the present invention, but the features of the present invention are not limited thereto, and all the scope of the present invention should be based on the following patent application, which is in accordance with the present invention. The spirit of the patent application and its similar variations are intended to be included in the scope of the present invention. Any change or modification that can be easily conceived in the field of the present invention can be covered in the following cases. Patent scope.

10‧‧‧Pesticide testing device

11‧‧‧ display screen

12‧‧‧Data input device

21‧‧‧LED light source

22‧‧‧ cuvette

23‧‧‧Light sensor

24‧‧‧Microcontroller

25‧‧‧ Analog Digital Converter

26‧‧‧Data storage device

27‧‧‧Battery

28‧‧‧clock generator

29‧‧‧ buzzer

30‧‧‧Power

Claims (13)

A pesticide detecting device comprising: an LED light source capable of generating a light path of a constant wavelength, wherein the predetermined wavelength corresponds to at least one pesticide; the cuvette is located on the light path, and has a container chamber for containing the pesticide a solution that allows light to pass through the light path; a light sensor positioned on the light path to receive light passing through the cuvette to generate a sensed signal; and a microcontroller electrically coupled to the light sensor to accept The sensing signal is based on pesticide residue interpretation. The pesticide detecting device according to claim 1, wherein the microcontroller electrically connects the LED light source to transmit a control signal to the LED light source. The pesticide detecting device according to claim 1, further comprising an analog digital converter electrically connected to the microcontroller and the photo sensor for converting the sensing signal into a digital signal for the microcontroller Analyze. The pesticide detecting device according to claim 1, wherein the light path between the LED light source and the cuvette includes a first focusing mirror to focus light on the cuvette. The pesticide detecting device according to claim 4, wherein the light path between the cuvette and the photo sensor includes a second focusing mirror to focus light on the photo sensor. The pesticide detecting device according to the first aspect of the patent application, further comprising a data input device electrically connected to the microcontroller. The pesticide detecting device according to claim 1 of the patent application, further comprising a display screen electrically connected to the microcontroller. The pesticide detecting device according to claim 1 of the patent application, further comprising a data storage device electrically connected to the microcontroller. The pesticide detecting device according to the first aspect of the patent application, further comprising a battery electrically connected to the microcontroller. The pesticide detecting device according to claim 1, wherein the LED light source is a constant wavelength light source having a wavelength of 350 to 450 nm. According to the pesticide detecting device of claim 1, the pesticide residue is interpreted as a result of comparison between a control group of a pesticide-free proteinase solution and a sample group of a protease-containing proteinase solution. According to the pesticide detecting device of claim 11, wherein the pesticide residue interpretation comprises using the acetylcholinesterase coloring method. According to the pesticide testing device of claim 11, wherein the pesticide residue interpretation is based on whether the inhibition rate of the sample group exceeds a critical value, wherein the inhibition rate = [(the slope of the control measurement value - the slope of the sample group measurement value) ) / slope of the measured value of the control group] × 100%.