TW201439313A - A cell detecting device - Google Patents

Abstract

A cell detecting device used to capture images during cell culturing process comprising a cell culturing container and a detecting module is disclosed. The cell culturing container includes a medium storage groove, a culturing groove and a ventilation groove. The medium storage groove has an opening connecting with the external environment and an assembly portion surround to form the opening. The cell culturing container further includes a sealing element with an opening end which is used to connect with the assembly portion. Both the culturing groove and the ventilation groove connects with the medium storage groove and has an opening connecting with the external environment the detecting module includes an image capturing unit and a controlling unit. The image capturing unit faces the culturing groove of the cell culturing container and the controlling unit electrically connects to the image capturing unit.

Description

Cell detection device

The present invention relates to a cell detecting device, and more particularly to a cell detecting device which can simultaneously pick up a cell growth image while culturing cells.

Cell culture technology is the core technology of the biotechnology industry. Cell culture in vitro is advantageous for observing various changes in cells during culture. The operator can add different compounds to the cultured cells, and observe the growth of the cells by observing the growth of the cells (for example, promoting apoptosis of cancer cells or promoting cell proliferation), thereby screening for possible new drugs. Compounds for subsequent animal and human testing.

Referring to FIG. 1 , it is a perspective exploded view of a conventional culture dish 9 which comprises a culture tank 91 and a lid 92 which can accommodate cells and the cover The body 92 can block the culture tank 91 to prevent foreign pollutants from entering the culture tank 91 during cell culture, resulting in cell contamination. The operator can suspend the cells in a liquid medium to form a cell suspension, and place the cell suspension in the culture tank 91 so that the cells can self-adhere to the bottom surface of the culture tank 91, and the operator can It is known that the culture dish 9 is placed in an incubator set to a specific temperature and humidity, and the cells can take up the nutrients contained in the liquid medium for growth.

When the growth state of the cells is to be observed, the operator can take out the conventional culture dish 9 from the incubator, open the lid body 92, take out the cells accommodated in the culture tank 91, and fix the cells on the slide glass. And with a microscope, observe with the naked eye, or take fine with photographic equipment The cell image is used to know the growth state of the cell.

However, if the operator needs to monitor the state of the cells for a long time, and during the monitoring period, the growth images of the cells are taken at regular intervals for subsequent analysis, and the aforementioned steps are cumbersome for the operator. This causes inconvenience.

Furthermore, for some relatively fragile cells (for example, primary cells isolated from living tissue), since the temperature and humidity of the growing environment are sensitive, if the operator frequently removes the conventional culture dish 9 from the incubator, It may change the physiological state of the cells, resulting in unstable test results or cell death.

Accordingly, it is indeed necessary to provide a cell detecting device to solve the above problems.

The main object of the present invention is to provide a cell detecting device which can be placed in an incubator and which can extract the growth images of the cells at a specific time point to enhance the convenience of the test.

A further object of the present invention is to provide a cell detecting device capable of extracting cell growth images at the same time as cell culture to avoid frequent changes in the cell growth environment, resulting in changes in the physiological state of the cells, thereby improving the stability of the test.

In order to achieve the foregoing object, the technical means and the achievable effects of the present invention include: a cell detecting device comprising: a cell culture device, and a receiving tank, the receiving groove Having an opening and a joint, the opening is in communication with the outside, the joint surrounds the opening, the cell culture device has a closure member, the closure member has an open end, and the closure member is coupled to the joint portion by the open end The cell culture device further comprises a culture tank and a gas tank, wherein the culture tank and the gas tank respectively communicate with the accommodating tank, the culture tank has a slot communicating with the outside, the gas tank has a ventilation with the outside And a detecting component disposed on the bottom surface of the cell culture device, the detecting component has an image capturing component and a control component, the image capturing component is paired in the culture tank of the cell culture device, and the control component is electrically The image capture component is connected.

In the cell detecting device of the present invention, the image capturing device is a CMOS image sensor or a CCD image sensor.

In the cell detecting device of the present invention, the culture substrate is coated with a culture substrate on the bottom surface thereof, and the culture substrate is poly-lysine.

The cell detecting device of the present invention, wherein the cell detecting device further comprises a filter, the filter is located between the cell culture device and the detecting component, and the filter pair is located in the cell culture device. Culture tank.

The cell detecting device of the present invention has a detecting component disposed on the bottom surface of the cell culture device, and the operator can obtain the growth image of the cell growth through the detecting component, thereby achieving the effect of improving the test convenience.

〔this invention〕

1‧‧‧cell culture

11‧‧‧ accommodating slots

111‧‧‧ openings

112‧‧‧Combination Department

12‧‧‧Closed

121‧‧‧Open end

122‧‧‧ accommodating space

13‧‧‧ culture tank

131‧‧‧ notch

14‧‧‧ gas trough

141‧‧ vents

2‧‧‧Detection components

21‧‧‧Image capture component

22‧‧‧Control elements

3‧‧‧Filter

C‧‧‧ cells

M‧‧‧Liquid medium

[study]

9‧‧‧ Petri dishes

91‧‧‧ incubator

92‧‧‧ cover

Figure 1 is a perspective exploded view of a conventional culture dish.

Fig. 2 is a perspective exploded view of the cell detecting device of the present invention.

Figure 3 is a top view of the cell detecting device of the present invention.

Fig. 4 is a cross-sectional view taken along line 4-4 of Fig. 3 of the cell detecting device of the present invention.

The above and other objects, features and advantages of the present invention will become more <RTIgt; "The surface of the cell detecting device that is relatively far from the ground when in use, that is, "above" in each drawing; the "bottom surface" as used in the present invention means the cell detecting device When using the state, the surface that is relatively far from the ground is the "lower" presented in each drawing.

The "stable environment" as used in the present invention refers to a constant temperature and constant humidity environment in which cells must be maintained during the culture process. It is understood by those skilled in the art that the temperature and humidity referred to by the stable environment vary depending on the cell type. It is also different.

Please refer to FIGS. 2 to 4, which are cell detecting devices according to a preferred embodiment of the present invention. The cell detecting device comprises a cell culture device 1 and a detecting component 2.

Preferably, the cell culture device 1 is selected to have a highly biocompatibility (PMMA) (Polymethy methacrylate) or glass as a substrate, and the cell culture device 1 is provided with a receiving tank 11 . The accommodating groove 11 has an opening 111 communicating with the outside. The accommodating groove 11 further has a joint portion 112. The joint portion 112 surrounds the opening 111. The cell culture device 1 has a closure member 12, and the closure member 12 has an open end 121, and an accommodating space 122 is formed in the sealing member 12, and the accommodating space 122 can be used for accommodating the liquid medium M required for cell culture, and the closing member 12 is coupled by the open end 121. The liquid medium M of the accommodating space 122 can flow into the accommodating groove 11 through the opening 111 of the accommodating groove 11 in the joint portion 112 of the accommodating groove 11 .

The cell culture device 1 is further provided with a culture tank 13 and a gas tank 14, and the culture tank 13 and the gas tank 14 are respectively connected to the accommodating tank 11, and the culture tank 13 and the gas tank 14 are preferably respectively located in the accommodating tank. 11 on the opposite side, but not limited to this. The culture tank 13 has a notch 13 communicating with the outside, and the culture tank 13 is used for culturing the cells C. Preferably, the bottom surface of the culture tank 13 can be coated with a culture medium so that the cells C can be attached to the cells. In the bottom surface of the culture tank 13, in the preferred embodiment, the medium is selected as poly-L-Lysine, but not limited thereto; the gas tank 14 has a connection with the outside. The vent 141, according to which the liquid medium M placed in the accommodating space 122 can flow to the accommodating tank 11, and then flows to the cultivating tank 13 and the gas tank 14 which are in communication with the accommodating tank 11, respectively. The culture tank 13 has the notch 131, and the air tank 14 has the vent 141, and the outside air system can simultaneously apply a pressure to the liquid medium M flowing into the culture tank 13 and the gas tank 14 to make the liquid medium M In the culture tank 13 and the gas tank 14 have a liquid level of equal height.

The detecting component 2 is disposed on the bottom surface of the cell culture device 1. The detecting component 2 has an image capturing component 21 and a control component 22, and the image capturing component 21 is aligned with the culture tank 13 of the cell culture device 1. The image capturing component 21 can capture a growth image of the cell C attached to the bottom surface of the culture tank 13. The image capturing component 21 can be selected as a CMOS (complementary metal-oxide semiconductor) image. Sensor or CCD (charge-coupled device) image sensor, the above CMOS image sensor and CCD image sensor have high image quality, high pixel, small size, power saving and low The control component 22 is electrically connected to the image capturing component 21; the operator can control the image capturing component 21 through the control component 22 to make the image capturing component 21 at a specific time. The image of the growth of the cell C located in the culture tank 13 is taken, and the image captured by the image capturing component 21 can be transmitted to an electronic device (not shown), and the user can immediately observe the cell C by the electronic device. Growth shadow For example, or subsequent analysis of the test results by the electronic device.

Preferably, the cell detecting device of the preferred embodiment further comprises a filter 3, which is located between the cell culture device 1 and the detecting component 2, preferably in the culture tank 13. The bottom surface, the filter 3 can be used to filter unnecessary light (for example, the red filter can filter out the shorter wavelength light, only the longer wavelength red light can pass), so that the image capturing component 21 can be used. An image of the light having a specific wavelength of the culture tank 13 is taken; preferably, the filter 3 is selected as a thin film filter.

Referring to FIG. 4, when the cell detecting device of the preferred embodiment is used, the operator can attach the cell C to the bottom surface of the culture tank 13, and then fill the accommodating space 122 of the sealing member 12 with the liquid medium. M, the sealing member 12 is coupled to the joint portion 112 of the accommodating groove 11 by the open end 122, so that the liquid medium M can flow from the accommodating space 122 into the accommodating groove 11, and the liquid medium M can flow into the accommodating groove 11 respectively. The tank 13 and the gas tank 14 are supplied to supply the fine The C is required to grow a nutrient source, and the operator can place the cell detection device in an incubator so that the cell C can grow in a stable environment.

During the cell culture process, the control signal stored in the control unit 22 is capable of manipulating the image capturing component 21 located in the culture tank 13, so that the image capturing component 21 can immediately capture the growth image of the cell C at each specific time point. According to this, the operator does not need to take out the cell detecting device of the preferred embodiment from the incubator, and the growth image of the cell C can be obtained.

In summary, the cell detecting device of the present invention has a detecting component disposed on the bottom surface of the cell culture device, and the operator can obtain the growth image of the cell growth through the detecting component, thereby achieving the effect of improving the test convenience.

Furthermore, since the cell detecting device of the present invention can capture the growth image of the cell through the detecting component, the cell growth environment can be avoided during the test, and the physiological state of the cell can be changed, thereby improving the stability of the test. efficacy.

While the invention has been described in connection with the preferred embodiments described above, it is not intended to limit the scope of the invention. The technical scope of the invention is protected, and therefore the scope of the invention is defined by the scope of the appended claims.

1‧‧‧cell culture

11‧‧‧ accommodating slots

111‧‧‧ openings

112‧‧‧Combination Department

12‧‧‧Closed

121‧‧‧Open end

122‧‧‧ accommodating space

13‧‧‧ culture tank

131‧‧‧ notch

14‧‧‧ gas trough

141‧‧ vents

2‧‧‧Detection components

21‧‧‧Image capture component

22‧‧‧Control elements

3‧‧‧Filter

Claims (7)

A cell detecting device comprises: a cell culture device, and a receiving groove, the receiving groove has an opening and a joint, the opening is in communication with the outside, the joint surrounds the opening, and the cell culture device has a a closure member having an open end, the closure member is coupled to the joint portion by the open end, the cell culture device is further provided with a culture tank and a gas tank, and the culture tank and the gas tank respectively communicate with the receiving tank The culture tank has a slot communicating with the outside, the air tank has a vent port communicating with the outside; and a detecting component is disposed on the bottom surface of the cell culture device, the detecting component has an image capturing component and a a control element, the image capturing element is paired with the culture tank of the cell culture device, and the control element is electrically connected to the image capturing element. The cell detecting device according to claim 1, wherein the image capturing device is a CMOS image sensor. The cell detecting device according to claim 1, wherein the image capturing device is a CCD image sensor. The cell detecting device according to the first aspect of the invention, wherein the bottom surface of the culture tank is coated with a culture substrate. The cell detecting device according to claim 4, wherein the culture medium is poly-lysine. The cell detecting device according to claim 1, wherein the cell detecting device further comprises a filter, the filter being located between the cell culture device and the detecting component. The cell detecting device according to claim 6, wherein the filter is in a culture tank located in the cell culture device.