KR101808063B1 - Cell incubator having plate for cell incubation - Google Patents

Cell incubator having plate for cell incubation Download PDF

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Publication number
KR101808063B1
KR101808063B1 KR1020150176246A KR20150176246A KR101808063B1 KR 101808063 B1 KR101808063 B1 KR 101808063B1 KR 1020150176246 A KR1020150176246 A KR 1020150176246A KR 20150176246 A KR20150176246 A KR 20150176246A KR 101808063 B1 KR101808063 B1 KR 101808063B1
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South Korea
Prior art keywords
plate
unit
cell culture
temperature
sub
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KR1020150176246A
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Korean (ko)
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KR20170069040A (en
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전병희
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주식회사 싸이토젠
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Publication of KR20170069040A publication Critical patent/KR20170069040A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A cell culture apparatus equipped with a cell culture unit according to an embodiment of the present invention includes a plate including a sub plate on which a plurality of wells for culturing cells are formed, And a control unit for controlling the inside of the culture apparatus, wherein the plate has a partition wall between each of the sub plates. The present invention also provides a cell culture apparatus comprising the cell culture unit.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a cell culture apparatus having a cell culture plate,

The present invention relates to a cell culture incubator having a cell culture plate, and more particularly, to a cell culture incubator having a temperature sensor on a cell culture plate to detect a temperature change on a subplate provided in the plate, To a cell culture incubator having a cell culture plate for performing monitoring on a plate.

Cell culture is a very important technology in biological research including molecular biology, which means culturing specific cells for diagnosis or treatment of human diseases. In general, cell culture is performed using a cell culture apparatus, which comprises a cover glass, a culture dish, and a cover.

Methods for culturing and observing cells using a conventional cell culture apparatus are as follows. Cells treated with drug or specific recombinant cells on a cover glass with a thin glass and placed in a culture dish are placed in a culture dish and then covered with a lid. The culture dish covered with the lid is introduced into an incubator, I'm ready. When incubation is completed, the incubator takes out the cover glass with a tweezers, places it on a glass slide, and observes it through a microscope. However, such a conventional method has the disadvantage that it is inconvenient to separately prepare a cover glass, a culture dish, and a lid, and there is a problem that a cell is likely to be contaminated during preparation for cell culture.

In addition, since the conventional method of injecting only one kind of culture liquid into the cover glass, it is difficult to treat various kinds of drugs at the same time, and in the process of attaching the cover glass to the glass slide to observe the cultured cells There is a problem that the cover glass breaks easily.

A cell culture apparatus including a plurality of chambers for culturing cells by simultaneously treating various kinds of drugs has been developed. However, such conventional cell culturing apparatuses including a plurality of chambers have a disadvantage that a method for sealing each chamber, There is a problem that the method for fixing the slide and the method for separating the fixed chamber from the glass slide are inconvenient and the glass slide is easily broken during the process of separating the chamber from the glass slide. In addition, there is a problem that the temperature of the slide changes and problems occur in the cell culture, or a change is detected, so that it can not cope quickly.

The technical problem to be solved by the present invention is to solve the problem that it is difficult to cope with the temperature change occurring during cultivation although the temperature can be heated for the condition for culturing the cells.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not intended to limit the invention to the precise form disclosed. There will be.

According to an aspect of the present invention, there is provided a cell culture apparatus comprising a cell culture unit according to an embodiment of the present invention, the cell culture apparatus comprising: a plate including a plurality of sub- A temperature sensor mounted on the sub-plate for sensing the temperature of each of the sub-plates; an imaging device for imaging the cells located in the well below the plate and in the well; A stage for moving the imaging device in X and Y axes; a pedestal for supporting the plate and moving the plate in the Z axis within a limited movement; a lens barrel positioned on the upper side of the plate and fixed to one side of the imaging device, an illumination unit for irradiating light to the wells, a control unit for controlling the inside of the incubation apparatus, Wherein the plate has partition walls between each of the sub plates, and when the control unit senses a temperature change, the control unit controls the image capturing unit to photograph the sub plate having a temperature change Thereby providing a cell culture apparatus.

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In the cell culture apparatus having the cell culture unit of the present invention, the temperature sensor may be a thermocouple.

Further, in the cell culture apparatus provided with the cell culture unit of the present invention, the control unit may receive the temperature change data from the temperature sensor and adjust the temperature of the subplate.

In addition, in the cell culture apparatus provided with the cell culture unit of the present invention, the control unit may set or adjust the initial temperature of the plate in accordance with the incubation temperature of the cells.

Further, in the cell culture apparatus provided with the cell culture unit of the present invention, the sub-plate may be detachable.

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In the cell culture apparatus provided with the cell culture unit of the present invention, the control unit may be connected to an interface unit provided outside the housing to control the imaging device unit and the illumination unit.

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According to an embodiment of the present invention, a temperature sensor is provided to detect a temperature change of a plate and monitor and cope with the temperature change.

It should be understood that the effects of the present invention are not limited to the above effects and include all effects that can be deduced from the detailed description of the present invention or the configuration of the invention described in the claims.

1 is a schematic diagram of a cell culture apparatus according to an embodiment of the present invention.
2 is a schematic view of a plate according to an embodiment of the present invention.
Fig. 3 is a schematic view showing a sub-plate attached to the plate of Fig.
4 is a schematic view of a sub-plate according to an embodiment of the present invention.
5 is a cross-sectional view of a plate according to an embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described with reference to the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and similar parts are denoted by like reference characters throughout the specification.

Throughout the specification, when a part is referred to as being "connected" (connected, connected, coupled) with another part, it is not only the case where it is "directly connected" "Is included. Also, when an element is referred to as "comprising ", it means that it can include other elements, not excluding other elements unless specifically stated otherwise.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, the terms "comprises" or "having" and the like refer to the presence of stated features, integers, steps, operations, elements, components, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

A cell culture apparatus 1 having a cell culture unit according to an embodiment of the present invention includes a plate including a sub plate 11 on which a plurality of wells 111 for culturing cells are formed And a temperature sensor 20 mounted on the plate 10 and sensing the temperature of each of the sub plates 11. The plate 10 has a partition wall between each of the sub plates 11, A cell culture apparatus (a cell culture apparatus) equipped with a cell culture unit for easily controlling the temperature of the sub-plate 11 by dropping each sub-plate 11 when a plurality of sub-plates 11 are mounted on the plate 10 1).

A cell culture apparatus 1 having a cell culture unit according to an embodiment of the present invention includes a plate 10 having a plurality of wells 111 for culturing cells, A plurality of temperature sensors 20 mounted at predetermined positions of the plate 10 to sense the temperature of the plate 10 and a plurality of temperature sensors 20 positioned below the plate 10 to photograph cells contained in the wells 111 And an illumination unit 50 disposed on the upper side of the plate 10 and fixed to one side of the imaging device unit 40 and for irradiating light to the well 111 , And the cell 10 is provided with a cell culture unit 1 in which the plate 10, the illumination unit 50 and the imaging device unit 40 are housed inside the housing 60 isolated from the outside .

The plate 10 may be manufactured by changing the size or shape according to the required level of the cell culture apparatus 1 and may be mounted with a single or a plurality of sub plates 11 in accordance with the number of the sub plates 11 to be mounted Or the like. Can be confirmed through the embodiment shown in FIG. 2 to FIG.

Since the sub-plate 11 is mounted on the plate 10, the shape or number of the cell or cell culture device to be cultured can be determined. The wells 111 formed in the sub-plate 11 may also be formed in the sub-plate 11 in a predetermined number or shape to a level required for cell culture. The imaging device unit 40 is disposed below the subplate 11. The imaging device unit 40 is disposed below the subplate 11 to observe or photograph the state of cell culture, (11) may be made of a transparent material such as plastic or glass.

According to the embodiment shown in FIGS. 3 and 4, the well 111 is formed in the sub-plate 11 in the form of a circle in the form of a circle, It is not excluded.

The temperature sensor 20 may be mounted at an edge or a vertex of each of the partitions of the plate 10 on which the sub-plate 11 is mounted. And may be connected to the control unit or the interface unit 61 of the cell culture apparatus 1 by a predetermined means.

The temperature sensor 20 may be a thermocouple, but may be a thermocouple type temperature sensor, a semiconductor resistance temperature sensor, a PTC thermistor, an NTC thermistor, a metal resistance type temperature sensor, a resistance temperature detector (RTD) It is not excluded that the device capable of measuring the temperature of the fluid.

The temperature sensor 20 may be located near the sub-plate 11 or the plate 10 as well as the bulkhead of the plate 10. [

The cell culture apparatus 1 further includes a control unit (not shown) and the control unit is connected to the imaging unit 40, the illumination unit 50, the interface unit 61, and the temperature sensor 20 by predetermined means So that it is possible to control the imaging device unit 40, the illumination unit 50, the interface unit 61, and the temperature sensor 20.

The control unit can adjust the temperature of the plate sensed by the temperature sensor 20 to an initial temperature required for culturing the cells before culturing the cells. When the cells start to be cultured, the temperature sensor 20 can measure the temperature change of the plate 10 or the sub-plate 11 and transmit the temperature change data to the control unit. The controller may determine the temperature change data received from the temperature sensor 20 and adjust the temperature inside the cell culture apparatus to adjust the temperature of the plate 10 or the subplate 11 to a level required for cell culture. The cell culture apparatus 1 may further include a temperature control unit for controlling the temperature of the control unit (not shown). The temperature regulating part may be composed of a Peltier element, but is not limited thereto.

When the temperature sensor 20 senses the temperature change of the sub-plate 11, the control unit captures the sub-plate 11 having a temperature change using the imaging device unit 40 of the cell culture apparatus 1 Or by observing the cells that are being cultured in the sub-plate 11.

1, the cell culture apparatus 1 includes a pedestal (not shown) capable of fixing the plate 10, and a fixing device (not shown) for fixing the imaging device unit 40 The stage 70 may move the imaging device unit 40 in the x and y axes to monitor the plate 10 being fixed.

In addition, a pedestal (not shown) is connected to the first body frame 80 inside the cell culture apparatus 1, and the imaging device unit 40 is disposed in the z- It allows movement, but it can limit movement in the z-axis, which can cause changes in the cultured cells. The stage 70 may be fixed to the second body frame 90 and the imaging device 40 may be fixed and installed on the upper surface of the stage 70.

The illumination unit 50 can be fixed to the stage 70 or the imaging device unit 40 by irradiating light to the well 111 in which the cells are cultured. The above-described components are isolated from the outside by the housing 60 and the housing 60 is provided with a control unit (not shown) or an interface unit 61 to check and control the change in the internal temperature or humidity .

The housing 60 further includes a cell culture apparatus 1 which can further inspect and inspect the inside of the housing 60 and can confirm the state of the inside of the housing 60 from the outside by using a front surface as a transparent material.

The controller 60 may further include a controller (not shown) to control the temperature and humidity of the interior of the housing 60. To this end, the controller 60 may further include a temperature controller and a humidity controller. The control unit may be connected to the temperature sensor 20 to measure the temperature of the plate 10 or the subplate 11. The control unit may measure the temperature of the plate 10 or the subplate 11 before the cells are cultured, ) Can be set or adjusted. In addition, the control unit is connected to the interface unit 61 by a predetermined means, and can display the culturing state of the cells through the interface unit 61 and the like.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

1: Cell culture device
10: Plate
11: Subplate
111: Well
20: Temperature sensor
40:
50:
60: Housing
61:
70: stage
80: first body frame
90: second body frame

Claims (10)

1. A cell culture apparatus comprising a cell culture unit,
A plate including a plurality of sub-plates in which a plurality of wells for culturing the cells are formed,
A temperature sensor mounted on the subplate and sensing the temperature of each of the subplates;
An imaging device unit positioned below the plate for capturing the cells contained in the well,
A stage unit coupled to the imaging device unit and configured to move the imaging device unit in X and Y axes,
A pedestal for supporting the plate and moving the plate in the Z-axis within a limited motion,
An illumination unit positioned above the plate and fixed to one side of the imaging device unit for irradiating light to the well,
And a control unit for controlling the inside of the culture apparatus and for controlling the imaging apparatus unit by judging temperature change data,
Wherein the plate has partition walls between each of the sub plates,
Wherein the control unit controls the imaging device unit to photograph a sub-plate having a temperature change when the temperature change is detected.
The method according to claim 1,
Wherein the temperature sensor is a thermocouple.
The method according to claim 1,
Wherein the control unit receives temperature change data from the temperature sensor and adjusts the temperature of the sub-plate.
The method according to claim 1,
Wherein the control unit sets or adjusts an initial temperature of the plate in accordance with the incubation temperature of the cell.
The method according to claim 1,
Wherein the sub-plate is detachable and attachable to the sub-plate.
delete delete delete The method according to claim 1,
Wherein the control unit is connected to an interface unit provided outside the housing to control the imaging device unit and the illumination unit.
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KR1020150176246A 2015-12-10 2015-12-10 Cell incubator having plate for cell incubation KR101808063B1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20240059448A (en) 2022-10-27 2024-05-07 (주)식중독실험기자재개발연구소 IoT-based cell culture device and automatic culture control method using the same

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102244188B1 (en) * 2017-12-28 2021-04-26 주식회사 싸이토젠 Time-lapse cell imaging apparatus
CN109182126A (en) * 2018-11-07 2019-01-11 中国医科大学附属盛京医院 A kind of cell Heating Experiment cell culture apparatus
KR102058826B1 (en) 2019-08-26 2019-12-23 한국광기술원 Cell culture and system and method for monitoring cell culture using the same
WO2023128714A1 (en) * 2021-12-30 2023-07-06 주식회사 매트릭셀바이오 Bioreactor and associated device

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009525756A (en) 2006-02-07 2009-07-16 ウェファージェン， インコーポレイテッド Temperature controlled culture plate
KR101377694B1 (en) 2013-05-06 2014-03-27 (주)실리콘화일 Device for analyzing cell and monitoring cell culture and method of analyzing cell and monitoring cell culture using the same
JP2014064507A (en) * 2012-09-25 2014-04-17 Sony Corp Culture container, culture observation apparatus, and culture observation method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009525756A (en) 2006-02-07 2009-07-16 ウェファージェン， インコーポレイテッド Temperature controlled culture plate
JP2014064507A (en) * 2012-09-25 2014-04-17 Sony Corp Culture container, culture observation apparatus, and culture observation method
KR101377694B1 (en) 2013-05-06 2014-03-27 (주)실리콘화일 Device for analyzing cell and monitoring cell culture and method of analyzing cell and monitoring cell culture using the same

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20240059448A (en) 2022-10-27 2024-05-07 (주)식중독실험기자재개발연구소 IoT-based cell culture device and automatic culture control method using the same

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