KR20240059448A - IoT-based cell culture device and automatic culture control method using the same - Google Patents

Abstract

This technology is an IoT-based cell culture device that selectively forms a plurality of partition spaces by partitioning the space within a housing having one space, and can culture, count, and experiment with cells such as bacteria in each partition space. Regarding this, more specifically, it includes a main body part that has a space part into which equipment for cultivating bacteria can be moved and a control part that interoperates with the experimenter terminal as well as a management server based on IoT, and an upper part of the main body part. An insulating screen is installed so that it can be unwound and divides the space into at least two parts to prevent interference by temperature in the partition space between each other, and is installed on the upper part of the main body to be located in the space, and cultivates bacteria in the equipment. It includes operating means controlled by a control unit to perform at least one of counting cultured bacteria and performing an experiment on cultured bacteria, wherein the operating means is limited to an experimenter terminal authenticated by the management server, remotely or by the control unit at a set time. It is characterized in that it can be operated through .

Description

IoT-based cell culture device and automatic culture control method using the same {IoT-based cell culture device and automatic culture control method using the same}

This technology is an IoT-based cell culture device that selectively forms a plurality of partition spaces by partitioning the space within a housing having one space, and can culture, count, and experiment with cells such as bacteria in each partition space. It's about.

In general, cell culture, such as bacteria, is a very important technology in biological research, including molecular biology, and means culturing specific cells for purposes such as diagnosis or treatment of human diseases.

Cell culture is performed using a cell culture device, and a conventional cell culture device consists of equipment including a cover glass, a culture dish, and a cover.

The method of culturing and observing cells using a conventional cell culture device is as follows.

Cell culture is performed by putting cells treated with drugs or cells with recombinant specific genes and culture medium on a thin glass cover glass, placing them in a culture dish, covering them with a cover, and placing the covered culture dish into an incubator. Finish your preparations.

The incubator proliferates (cultures) cells by creating a specific environment suitable for the purpose of cell culture. Once cell proliferation is completed, the incubator first moves to the counting laboratory and counts the cells grown in the culture dish with the naked eye and a type of camera. This is confirmed with a colony counter, and once a certain number of counts is obtained, the suspected cells must be moved to the laboratory again for the purpose of another experiment, and all transfer processes must be carried out under conditions that do not affect the proliferated cells. do.

Meanwhile, in order to perform the above-mentioned culture process, counting process, and experiment process, the experimenter must not only open and close the culture dish one by one, but also must not affect the cells grown in the culture dish during the movement process, so it is important for the entire laboratory. There is the inconvenience of having to maintain a constant indoor temperature using sterile conditions and an air conditioning system at all times.

In addition, in order to obtain precise experimental results, the experimenter inevitably has to conduct the experiment at night. In this process, errors may occur in the experimental results due to the experimenter's fatigue, or the petri dish may be damaged due to careless handling. Alternatively, there is a problem that contamination may frequently occur due to frequent opening and closing of the cover.

Domestic registered patent No. 10-1808063 (announcement date: 2017.12.14.) Domestic registered patent No. 10-1798501 (announcement date: 2017.11.16.) Domestic registered patent No. 10-2441836 (announcement date: 2022.09.08.)

In order to solve the above-described conventional problems, the present invention satisfies a structure that can arbitrarily partition the space inside one incubator, and moves equipment such as a culture dish back and forth inside the partitioned space to carry out the process of culturing, counting, and experimentation. The purpose is to allow the process to proceed without any effect on cell deterioration or damage.

In addition, another purpose is to enable the experimenter to automatically proceed with the process of cultivating cells remotely or using set information based on the Internet of Things, thereby satisfying highly reliable experimental results regardless of fatigue.

In order to solve the above-mentioned technical problems, the IoT-based microbial culture device according to the present invention has a space part 111 into which equipment (P/D) for cultivating bacteria can move, and has a management server ( 500), as well as a main body 100 equipped with a control unit 400 that interoperates with the experimenter terminal 600, and is removably installed on the upper part of the main body 100, so that the space 111 has at least two An insulation screen 200 is installed on the upper part of the main body 100 to be located in the space 111 and an insulation screen 200 is divided into more than one space to prevent interference by temperature in the partition space 113, and equipment (P/D) It includes operating means 300 controlled by the control unit 400 to perform at least one of cultivating bacteria, counting bacteria cultured on equipment (P/D), and performing an experiment on cultured bacteria, wherein the operation means The means 300 is characterized in that it allows only the experimenter terminal 600 authenticated by the management server 500 to operate through the control unit 400 remotely or at a set time.

In addition, the main body 100 is provided with a window 115 that can be seen from the outside, and the space 111 having an open and closeable door 117 is formed inside, and the operating means 300 is located on the inner upper part. This installed housing 110 and a conveyor 120 rotatably provided inside the housing 110 to move the equipment (P/D) in a reciprocating manner by rotation, are unwound on the upper part of the housing 110. It is preferable that the insulation screen 200 is formed with a through hole 119 through which the insulation screen 200 is inserted to partition the space 111.

In addition, the insulating screen 200 is installed on the upper surface of the housing 110 and has a case 210 with a screen motor 211 on one side and a through hole 119 according to the rotation direction of the screen motor 211. It includes a screen 220 that is unwound in the shape of a plate within the space 111 to form a plurality of partition spaces 113 or wound in a roll shape inside the case 210, wherein the screen ( 220) is made of an insulating material, and the screen 220 is wound to a predetermined height when the conveyor 120 is driven and is preferably controlled by the control unit 400 to prevent interference with the movement of equipment (P/D). do.

In addition, the insulation screen 200 is installed in a plurality of configurations adjacent to each other on the upper surface of the housing 110 so that at least three partition spaces 113 divided by the screen 220 can be provided, and 3 The compartment space 113 is preferably used as a culture space, counting space, and experiment space.

In addition, the operating means 300 installed in the housing 110 includes a culture operation unit 310 installed in the culture space partitioned by the insulation screen 200, and a culture operation unit 310 partitioned by the insulation screen 200. By the counting operation unit 320 and the insulation screen 200, which are installed in a counting space adjacent to the space and photograph bacteria cultured in equipment (P/D) and then transmit the data to the control unit 400 to obtain counting information. It includes at least one of an experimental operation unit 310' that is installed in a partitioned experimental space adjacent to the counting space and performs an experiment using bacteria identified as suspected colonies, wherein the housing 110 includes a culture space, a counting space, It is preferable to include a plurality of cameras 350 that independently photograph the experimental space and transmit the acquired photographic information to the control unit 400.

In addition, the culture operation unit 310 and the experiment operation unit 310' include a tray 311 installed to be able to be raised and lowered along a rail (R) installed on the inner wall of the housing 110, and the tray 311 is raised and lowered. It includes a lifting member 312 that provides the power to do so, and a moving member 330 installed inside the tray 311 to be able to move back and forth in the X-axis and Y-axis directions so as to be located on top of the equipment (P/D). It is desirable to do so.

In addition, the counting operation unit 320 is installed on the upper part of the housing 110, and is connected to an elevating cylinder 321 through which a rod whose length is adjustable is inserted into the counting space, and with the rod of the elevating cylinder 321, An elevating bracket 322 that goes up and down within the elevating bracket 322, a colony counter 324 provided at the lower part of the elevating bracket 322 and moving to the length of the elevating bracket 322 along a moving hole 323, and the colony counter 324 ) and the screw 326 are installed on the lifting bracket 322 so that they can engage with each other, and a screw motor ( 325), it is desirable to include it.

According to the present invention, differently from the conventional case, the space formed inside the housing is arbitrarily divided into a plurality of partition spaces by an insulating screen to have different culture spaces, counting spaces, and experiment spaces for cells, and at the same time, each space is separated through a conveyor. It is possible to move sequentially through the compartment space, so it is expected that it can be used without affecting the deterioration of cells or damage to equipment.

In addition, unlike in the past, based on IoT, the control unit provided in the culture device can be remotely or automatically controlled through the experimenter's terminal with approval from the management server, ensuring highly reliable experiment results regardless of the experimenter's fatigue. I am looking forward to the possible effects.

1 is a system conceptual diagram of an IoT-based cell culture device according to the present invention.
Figure 2 is a perspective view showing an IoT-based cell culture device according to the present invention.
Figure 3 is an exploded view of Figure 2.
Figure 4 is an exploded view of the main body of Figure 2.
Figure 5 is an exploded view showing the culture operation unit or the experiment operation unit for Figure 2.
Figure 6 is a diagram illustrating the working relationship of the lifting member with respect to Figure 5.
Figure 7 is a diagram illustrating the operational relationship of the X-axis movement means in Figure 5.
Figure 8 is an operational relationship diagram of the Y-axis moving means in Figure 5.
Figure 9 is a perspective view showing the experimental operation unit for Figure 5.
Figure 10 is a perspective view showing the counting operation unit for Figure 2.
Figure 11 is an operational relationship diagram of the counting operation unit in Figure 10.
Figure 12 is an internal view of Figure 2.
Figure 13 is a flowchart showing an automatic culture control method using an IoT-based cell culture device according to the present invention.
Figure 14 is a conceptual diagram showing the connection relationship of the operating means by the mode selection unit with respect to Figure 13.
Figure 15 is a diagram showing an experimenter's terminal with the setting mode for Figure 13 displayed.

Hereinafter, various embodiments will be described in more detail with reference to the attached drawings. The embodiments described herein may be modified in various ways. Specific embodiments may be depicted in the drawings and described in detail in the detailed description. However, the specific embodiments disclosed in the attached drawings are only intended to facilitate understanding of the various embodiments. Therefore, the technical idea is not limited to the specific embodiments disclosed in the attached drawings, and should be understood to include all bacterial equivalents or substitutes included in the spirit and technical scope of the invention.

Terms containing ordinal numbers, such as first, second, etc., may be used to describe various components, but these components are not limited by the above-mentioned terms. The above-mentioned terms are used only for the purpose of distinguishing one component from another.

In this specification, terms such as “comprise” or “have” are intended to designate the presence of features, numbers, steps, operations, components, parts, or combinations thereof described in the specification, but are not intended to indicate the presence of one or more other features. It should be understood that this does not exclude in advance the possibility of the existence or addition of elements, numbers, steps, operations, components, parts, or combinations thereof. When a component is said to be "connected" or "connected" to another component, it is understood that it may be directly connected to or connected to the other component, but that other components may exist in between. It should be. On the other hand, when it is mentioned that a component is “directly connected” or “directly connected” to another component, it should be understood that there are no other components in between.

In addition, when describing the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the gist of the present invention, the detailed description thereof is abbreviated or omitted.

Hereinafter, the IoT-based cell culture device (hereinafter simply referred to as 'culture device') according to the present invention will be described in detail with reference to the attached drawings.

Prior to explanation, the cells described below will be explained using culturable food poisoning bacteria as an example.

First, as shown in FIGS. 2, 3, and 12, the culture device 1 according to the present invention largely includes a main body 100, an insulating screen 200, an operating means 300, and one or more of these components. It includes a control unit 400 that electrically controls, and the control unit 400 has the structure of a typical control panel equipped with a plurality of switches and displays, so detailed description will be omitted so as not to obscure the gist of the present invention. .

However, as will be explained later, the control unit 400 in the present invention is linked to the management server 500 and the experimenter terminal 600 using a wired and wireless communication network to remotely control the experimenter terminal 600 from a distance. Note that this is possible (see Figure 1).

In more detail, as shown in FIGS. 3 and 4, the main body 100 is used to culture, count, and experiment with food poisoning bacteria using accessible equipment (P/D) such as a petri dish. It is configured to provide a space for movement and includes a housing 110 and a conveyor 120.

For example, as shown, the housing 110 is molded in the shape of a hexahedron with a space 111 formed on the inside, and a window 115 that can be seen from the outside is installed on the outer surface, so that the housing 110 can be cultured through equipment (P/D). Allows the experimenter to visually check the cells during the counting and experiment process.

The upper surface of the housing 110 has a through hole 119 for the screen 220 to enter and exit so that the insulating screen 200, which will be described later, can be expanded to have the shape of a plate inside the space 111 and rolled back to its original state. Perforations may be formed according to the number of the insulation screens 200.

Through this, the space 111 can be divided into a plurality of partition spaces 113 and divided into a culture space, a counting space, and an experiment space, and this will be explained in more detail in the description of the insulation screen 200. .

A window 115 is formed on the outer surface of the housing 110, as well as at least one door 117 on the outer surface where the window 115 is not installed to facilitate entry and exit of equipment (P/D) or maintenance of the conveyor 120. Make repairs possible.

In addition, the conveyor 120 refers to a plurality of rollers rotated by a motor and a typical conveyor 120 that is wound around the outer surface of the rollers and can move back and forth in the X-axis direction according to the rotation direction of the motor (see Figure 12). .

Through the movement of the conveyor 120, equipment (P/D) moves within the space 111 divided into a plurality of partition spaces 113, thereby enabling cell culture, counting, and experiments.

In addition, a plurality of the insulation screens 200 are installed adjacent to each other in the longitudinal direction on the upper surface of the housing 110 described above to divide the space 111 into at least two partition spaces 113, and the case 210 and screen 220 (see FIG. 12).

For example, the case 210 is equipped with a screen 220 wound in the form of a scroll inside so that it can be unwound to the outside, and on the side, the screen 220 is rotated and spread out to have the shape of a plate in the direction of the space 111. Or, it can be wound back to its original state.

For this purpose, the end of the screen 220 that is unwound to the outside of the case 210 is expanded into the space 111 through the through hole 119, and in the unfolded state, the space 111 is divided into a plurality of partition spaces ( 113).

At this time, the preferred number of partition spaces 113 in the present invention is a total of three, and for this purpose, two insulating screens 200 are preferably provided on the upper part of the housing 110.

In addition, the screen 220 may be made of an insulating material so that the environment, such as temperature, does not interfere with each other between the partition spaces 113, or a coating material (not shown) for insulation may be applied to the outer surface of the screen 220.

Through the insulating screen 200 in the present invention, the experimenter spreads one or more screens 220 to form a plurality of partition spaces 113, thereby forming a plurality of partition spaces 113, so that the integration and counting of the culture space and the counting space are performed according to the nature of the cells being cultured. It is possible to separate the space and the experiment space as well as the culture space, the counting space, and the experiment space. In order to clearly explain the gist of the present invention, a total of three sections are divided in the order of the culture space, counting space, and experiment space. This will be explained using the partition space 113 as an example.

In addition, as shown in FIGS. 5 to 11, the operating means 300 is installed on the upper part of the housing 110 so that it can be arranged in each of the three partition spaces 113 to enable cell culture, counting, and experiments. It is configured to be easily executed and includes a culture operation unit 310 installed in the culture space, a counting operation unit 320 installed in the counting space, and an experiment operation unit 310' installed in the experiment space, as shown. As shown, the culture operation unit 310 and the experiment operation unit 310' differ only in the location of the rail (R) installed on the housing 110, and have the same mechanical configuration and structure, so the culture operation unit ( The explanation of the experimental operation unit 310' will be replaced by the explanation of 310) (see FIG. 12).

However, while the culture operation unit 310 in the present invention is used for cultivating cells on equipment (P/D), the experiment operation unit 310' is a cultured cell sample, that is, a sample identified as a suspicious colony. Note that there is a difference in that it is used for inoculating other cells (or bacteria) for biochemical experiments (see Figure 9).

For example, as shown in FIGS. 5 to 8, the culture operation unit 310 is configured to culture samples on a plurality of equipment (P/D) by lifting and moving back and forth in the X-axis and Y-axis directions within the culture space. It includes a tray 311, a lifting member 312, and a moving member 330.

As shown, the tray 311 is molded into a square strip shape with a hollow portion on the inside, and has a structure on its outer surface that is raised and lowered by a lifting member 312 along a rail (R) formed on the inner wall of the housing 110. .

Here, the elevating member 312 is located in the hollow portion of the tray 311, and the tray 311 itself is elevated along the rail R so that the culture body 340 through which the moving member 330 is inserted can be elevated. The configuration for providing power for this includes a lifting motor 313 and a lifting screw 316.

The lifting motor 313 is rotatably installed in the housing 110 to be located on the upper part of the tray 311, and a driving pulley 314 is coupled to the driving shaft of the lifting motor 313 to provide a rotating force to the driven pulley 315. Deliver.

For this purpose, the driving pulley 314 and the driven pulley 315 are installed to be surrounded by a belt (B) with gears formed on the inner surface so as to be tooth-coupled with each other, and a lifting screw ( 316) is installed to extend downward so that the rotational force of the lifting motor 313 is transmitted through the driving pulley 314, the belt B, and the driven pulley 315.

At this time, the thread formed on the outer surface of the lifting screw 316 is tooth-coupled with the outer surface of the tray 311 to provide power to elevate the tray 311 according to the rotation direction of the lifting screw 316. It is desirable.

The moving member 330 is configured to reciprocate the culture body 340 located in the hollow portion of the tray 311 in the X-axis and Y-axis directions. For this purpose, the moving member 330 includes and Y-axis moving means 332.

Here, the X-axis moving means 331 is configured to reciprocate the culture body 340 in the It includes a pair of X-axis rods (333b) rotatably coupled to the shaft motor (333a).

For this purpose, as shown, the They are each rotatably installed on surfaces facing each other, and are connected to each other by a plurality of belts (B), satisfying a structure in which a pair rotates by the rotation of the X-axis drive motor (333a).

At this time, on the outer surface of the pair of X-axis rods 333b, a pair of It is desirable to satisfy the structure and reciprocate in the +X axis or -X axis direction depending on the rotation direction of the X axis driving motor 333a.

The Y-axis moving means 332 has the same configuration as the X-axis moving means 331 described above. In the case of the Y-axis moving means 332, as shown, a pair of Y-axis rods 334b are connected to the They are connected to each other by a belt (B) so that they face each other on a different side from the rod (333b), so that the culture body (340) moves in the +Y-axis or -Y-axis direction depending on the rotation direction of the Y-axis motor (333a). It is installed on the tray 311 so that it can move back and forth.

In addition, a pair of Y-axis connectors 338 facing each other are installed on the pair of Y-axis rods 334b so that they can be disposed on both sides of the Y-axis connection bar 337, similar to the X-axis moving means 331 described above. In addition, the Y-axis connection bar 337 is also installed to penetrate the culture body 340.

At this time, the culture medium 340 is preferably connected through a pipe (not shown) whose length can be adjusted and bent so that the culture medium or test liquid (not shown) can be supplied from the outside and inoculated into the equipment (P/D).

Furthermore, the penetrates so that

In addition, as shown in FIGS. 10 and 11, the counting operation unit 320 is configured to photograph the equipment (P/D) transported to the counting space and count the samples cultured in the equipment (P/D). It includes a lifting cylinder 321, a lifting bracket 322, a colony counter 324, and a screw motor 325.

As shown, the lifting cylinder 321 is fixedly installed on the upper surface of the housing 110, and a rod of variable length penetrates the housing 110 and is fastened to the lifting bracket 322, through which the lifting bracket 322 ) satisfies the structure that can be lifted up and down.

On the outer surface of the lifting bracket 322 fastened to the lifting cylinder 321, a colony counter 324 is inserted through a moving hole 323 so that it can move along the longitudinal direction of the lifting bracket 322, and the colony counter inserted through (324) The upper part is inserted through the screws 326 so that they can engage with each other.

Here, one side of the screw 326 that engages the colony counter 324 is coupled so that it can rotate in both directions by the screw motor 325, and moves to the moving hole 323 according to the rotation direction of the screw motor 325. It satisfies the structure in which the colony counter 324 is guided to move to Oabok.

The colony counter refers to a camera that moves and elevates through the above-described counting operation unit 320 to zoom in and take pictures of cells cultured in equipment (P/D) down to the micro unit. Counting is possible, and for this purpose, the imaging information of the colony counter 324 is transmitted to the control unit 400 and the number of cells is counted through a predetermined algorithm.

At this time, the control unit 400 stores the photographed information captured through the colony counter 324 and transmits the photographed information when requested by the experimenter terminal 600 so that the experimenter can check the counting information from the outside. It is also possible to control the counting operation unit 320 itself in real time to obtain additional status information of cells cultured in the equipment (P/D).

Meanwhile, the operating means 300 in the present invention may further include a camera 350 for photographing the entire interior of the partition space 113, and the photographing information photographed through the camera 350 is transmitted to the control unit 400. It can be stored and managed in the database of the control unit 400 or management server 500 so that it can be transmitted to and confirmed through the experimenter terminal 600.

Hereinafter, the automatic culture control method (hereinafter simply referred to as the 'control method') using an IoT-based cell culture device according to the present invention will be described in detail with reference to the attached drawings.

Prior to explanation, in order to clearly understand the gist of the present invention, the control method (2) described below is not a conventional method in which the experimenter manually operates the control unit 400, but is performed using an experimenter terminal (600) owned by the experimenter. ), and to this end, the experimenter terminal 600 controls the control unit 400 using an application provided to the management server 500. This will be explained using examples.

First, as shown in FIGS. 13 to 15, in order to remotely control the culture device 1 according to the present invention by the experimenter terminal 600, 1) the culture device 1 is controlled through the experimenter terminal 600. Step (S10) of receiving authentication information for remotely operating the control unit 400 from the management server 500, 2) cultivation mode (M1-1) and counting mode (M1-2) through the experimenter terminal 600 , selecting one of the experimental modes (M1-3) (S20), and 3) receiving and checking real-time shooting information and current information for the selected mode from the control unit 400 to the experimenter terminal 600. Includes (S30).

To explain in more detail, for step 1), the experimenter logs in using the application installed on the experimenter terminal 600 and receives authentication information to control the desired culture device 1 from the management server 500.

Authentication information provided by the management server 500 can use the serial number or unique number assigned to each culture device 1, and for this purpose, the experimenter can use multiple culture devices 1 displayed on the experimenter terminal 600. When one of the options is selected, authentication information for the selected culture device 1 is requested from the management server 500, and the experimenter inputs the authentication information he or she knows to control the control unit 400 of the desired culture device 1. You will be given permission to access (see Figure 1).

When permission is granted through step 1) described above, a mode selection unit including culture mode (M1-1), counting mode (M1-2), and experiment mode (M1-3) is displayed on the experimenter terminal 600, The experimenter executes step 2), selecting one of the displayed modes.

Here, the culture mode (M1-1) refers to a series of operations that can operate the culture operation unit 310 to perform inoculation for cell culture using equipment (P/D) in the culture space, The counting mode (M1-2) allows the colony counter 324 to count the number of enlarged cell colonies being cultured on equipment (P/D) transported by the conveyor 120 from the culture space to the counting space. It refers to a series of operations that can operate the counting operation unit 320, and the experimental mode (M1-3) is limited to cells identified as suspicious colonies among cells transported by the conveyor 120 from the counting space to the experimental space. It refers to a series of operations that can manipulate the experimental operation unit 310' to perform a biochemical experiment (see FIG. 12).

Afterwards, when step 2), in which one mode is selected by the experimenter and the work is done remotely, is completed in step 3)), the control unit 400 transmits the current information indicating the result to the experimenter terminal 600. Allow the experimenter to check.

Here, the current information may include bacterial culture information cultivated in the culture space, bacterial counting information counted by the colony counter 324 in the counting space, and bacterial test information on bacteria identified as suspicious colonies in the experimental space.

In addition, in step 3), apart from the current information, it is possible to additionally provide real-time shooting information of one or more of the culture space, counting space, and experiment space photographed by the camera 350.

Meanwhile, the control method (2) in the present invention may further include step 4) (S40) after step 3), and step 4) is performed through the experimenter terminal 600 according to the results of the confirmed current information. This refers to the step of selecting a setting mode to change the setting value of the control unit 400.

Here, the setting mode is a temperature control mode (M2-1) that controls different temperatures for each compartment 113 and an operation time mode (M2-1) that controls the operation time of the operating means 300 at different times for each compartment. 2) may be included.

The temperature control mode (M2-1) is operated according to the space 111 or each compartment space 113 through an air conditioning device (not shown) in communication with the culture device 1 of the present invention based on a temperature sensor not shown. Different temperatures can be set.

The operation time mode (M2-2) is a culture time setting that sets the operation time of the culture operation unit 310, a counting time setting that sets the operation time of the counting operation unit 320, and an operation time of the experiment operation unit 310'. Includes experiment time settings to set.

That is, step 4) in the present invention is a process in which the experimenter individually controls the culture operation unit 310, the counting operation unit 320, and the experiment operation unit 310' remotely through the control unit 400, as described. The control unit 400 learns about the process and stores it as data, and when a repetitive task is performed, it is automatically executed through the operation time mode (M2-2) rather than remotely by the experimenter. It becomes possible.

Furthermore, the results of tasks automatically executed through step 4) are transmitted to the experimenter terminal 600 in the form of data and can be confirmed in real time.

As described above, the culture device 1 according to the present invention is different from the conventional one by arbitrarily dividing the space 111 formed inside the housing 110 into a plurality of compartment spaces 113 by means of an insulating screen 200. It is divided into different culture spaces, counting spaces, and experiment spaces for cells, and at the same time, sequential movement to each compartment space (113) is possible through the conveyor (120), preventing deterioration of cells or damage to equipment (P/D). It is expected that the effect can be used without any influence on the user.

In addition, differently from the prior art, the control unit 400 provided in the culture device 1 is controlled remotely or automatically through the experimenter terminal 600 based on IoT (Internet of Things) with approval from the management server 500. Therefore, the effect of satisfying highly reliable experimental results is expected regardless of the experimenter's fatigue.

As described above, the present invention has been described with specific details such as specific components and limited embodiments and drawings, but this is only provided to facilitate a more general understanding of the present invention, and the present invention is not limited to the above embodiments. , those skilled in the art can make various modifications and variations from this description.

Therefore, the spirit of the present invention should not be limited to the described embodiments, and the scope of the patent claims described below as well as all things that are equivalent or have equivalent modifications to the scope of this patent claim are said to fall within the scope of the spirit of the present invention. will be.

1: IoT-based cell culture device according to the present invention
2: Automatic culture control method using IoT-based cell culture device according to the present invention
100: main body
110: Housing 120: Conveyor
200: Insulating screen
210: case 220: screen
300: Operating means
310: Culture operation unit 310': Experiment operation unit
320: counting operation unit 330: moving member
340: Culture 350: Camera
400: Control unit
500: Management server
600: Experimenter terminal

Claims (10)

It has a space part 111 where equipment (P/D) for cultivating bacteria can be moved, and a control unit 400 that is interconnected with the experimenter terminal 600 as well as the management server 500 based on IoT. Provided main body 100;
An insulating screen 200 that is removably installed on the upper part of the main body 100 and divides the space 111 into at least two parts to prevent interference by temperature in the partition spaces 113 between each other. and
It is installed on the upper part of the main body 100 so as to be located in the space 111, and at least one of the following: cultivating bacteria on the equipment (P/D), counting the bacteria cultured on the equipment (P/D), and testing the cultured bacteria. Includes an operating means 300 controlled by the control unit 400 to execute any one operation,
The operating means 300 is an IoT-based cell culture device characterized in that it can be operated through the control unit 400 remotely or at a set time only by the experimenter terminal 600 authenticated by the management server 500.
According to paragraph 1,
The main body 100 is
A housing 110 having a window 115 that can be seen from the outside and a space 111 having an openable door 117 formed therein, and the operating means 300 installed on the inner upper portion; and
A conveyor 120 is rotatably provided inside the housing 110 and moves the equipment (P/D) in a reciprocating manner by rotation,
An IoT-based cell culture device, characterized in that a through hole 119 is formed in the upper part of the housing 110 through which the unrolled insulating screen 200 is inserted to partition the space 111.
According to paragraph 2,
The insulation screen 200 is
A case 210 installed on the upper surface of the housing 110 and having a screen motor 211 on one side; and
Depending on the rotation direction of the screen motor 211, it is released into the shape of a plate within the space 111 through the through hole 119 to form a plurality of partition spaces 113, or a roll ( Including a screen 220 wound in the form of a roll,
The screen 220 is made of an insulating material, and the screen 220 is wound to a predetermined height when the conveyor 120 is driven and is controlled by the control unit 400 to prevent interference with the movement of the equipment (P/D). An IoT-based cell culture device characterized by
According to paragraph 3,
The insulation screen 200 is
A plurality of partition spaces 113 divided by the screen 220 are installed adjacent to each other on the upper surface of the housing 110 in a plurality of configurations so that at least three partition spaces 113 are provided, and the three partition spaces 113 are culture spaces. , IoT-based cell culture device characterized by being used as a counting space and experiment space.
According to paragraph 2,
The operating means 300 installed in the housing 110 is
A culture operation unit 310 installed in the culture space partitioned by the insulation screen 200;
A counting operation unit ( 320); and
An experiment operation unit 310' that is partitioned by the insulation screen 200 and is installed in an experiment space adjacent to the counting space to perform an experiment using bacteria identified as suspicious colonies; Include at least one of the following,
The housing 110 is an IoT-based cell culture device characterized in that it includes a plurality of cameras 350 that independently photograph the culture space, counting space, and experiment space and transmit the acquired photographic information to the control unit 400. .
According to clause 5,
The culture operation unit 310 and the experiment operation unit 310' are
A tray 311 installed to be able to be lifted up and down along a rail (R) installed on the inner wall of the housing 110;
an elevating member 312 that provides power to elevate the tray 311; and
An IoT-based cell culture device comprising a moving member 330 installed inside the tray 311 to be movable in the X- and Y-axis directions so as to be located on top of the equipment (P/D). .
According to clause 5,
The counting operation unit 320 is
An elevating cylinder 321 installed on the upper part of the housing 110 and into which a rod whose length is adjustable is inserted into the counting space.
A lifting bracket 322 that is engaged with the rod of the lifting cylinder 321 and moves up and down within the counting space;
A colony counter 324 is provided at the lower part of the lifting bracket 322 and moves along the moving hole 323 to the length of the lifting bracket 322; and
The colony counter 324 and the screw 326 are installed on the lifting bracket 322 so that they can engage with each other, and the power for moving the colony counter 324 is provided by rotating the screw 326. An IoT-based cell culture device comprising a screw motor 325.
1) Receiving authentication information for remotely operating the control unit 400 of the culture device 1 through the experimenter terminal 600 from the management server 500;
2) Executing the work remotely by selecting any one of the culture mode (M1-1), counting mode (M1-2), and experiment mode (M1-3) through the experimenter terminal 600; and
3) receiving and confirming the current information about the selected mode from the control unit 400 to the experimenter terminal 600;
The current information in step 3) is at least one of the following: bacterial culture information cultivated in the culture space, bacterial count information counted by the colony counter 324 in the counting space, and bacterial test information on bacteria identified as suspicious colonies in the experimental space. An automatic culture control method using an IoT-based cell culture device, characterized in that:
According to clause 8,
After step 3), proceed to step 4),
It includes: selecting a setting mode through the experimenter terminal 600 according to the results of the current information,
The setting mode is a temperature control mode (M2-1) that controls different temperatures for each compartment 113 and an operation time mode (M2-2) that controls the operation time of the operating means 300 at different times for each compartment. An automatic culture control method using an IoT-based cell culture device, characterized in that at least one of the following is further included.
According to clause 9,
The operation time mode (M2-2) is
Culture time setting to set the operation time of the culture operation unit 310;
Counting time setting to set the operation time of the counting operation unit 320; and
An automatic culture control method using an IoT-based cell culture device, comprising: experiment time setting for setting the operation time of the experiment operation unit 310'.

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