TW201422810A - Modularized photosynthetic reactor system, algae culturing method using the same and clamp set - Google Patents

Abstract

The present disclosure relates to a modularized photosynthetic reactor system and an algae culturing method using the same, in which the modularized photosynthetic reactor system includes at least one reactor, an air pipe, an out-feed tube, an in-feed tube and an exhausting pipe. Moreover, each reactor is composed of a plurality of clamp sets and at least one membrane cartridge, and the algae culturing method comprises: a sterilizing procedure, a culturing procedure and a harvesting procedure. In the sterilizing procedure, water, air and a hypochlorite solution are fed into a reactor for sterilization and then a sodium thiosulfate solution is fed into the same reactor for chlorine neutralization. In the culturing procedure, a culture medium and an algae liquid are fed into the reactor for allowing algae to grow until a steady growth period is reached. In the harvesting procedure, the algae grown in the reactor is harvested after the steady growth period is ended.

Description

Modular photosynthetic bioreactor system, algae culture method and fixture set

The present disclosure relates to a modular photosynthetic bioreactor system and an algae culture method, and more particularly to a modular photosynthetic bioreactor system in which a reactor is formed by a clamp ring group and a film cylinder to make the reactor have a closed property. Algae farming methods.

Conventional microalgae culture systems are mostly cultivated in open culture ponds. However, in order to increase the unit yield of microalgae and avoid the invasion of exotic algae, the development of photosynthetic bioreactors has received increasing attention in recent years. Generally, the body of the photosynthetic bioreactor is made of a transparent material, such as glass or polymethyl methacrylate (acrylic), polyvinyl chloride, polycarbonate, and the like. Although the bioreactor can be produced by using the above materials to obtain a good microalgae cultivation effect, the production cost is difficult to meet the demand of biomass energy.

For example, a two-piece hot-pressed transparent plastic film is combined into a sheet type photosynthetic reactor. The width of the reactor is thin, and the culture liquid and the introduced gas are thoroughly mixed in the flow path of the reactor to achieve a high-density culture effect of the microalgae. However, the culture is small and not suitable for large-scale farming.

Another example is a flat bag reactor developed in a water environment. Although the bag reactor in the water body does not require a support frame and has high environmental stability, it must be limited by the pool equipment, and the reactor in the water body will also increase the light. Transparent barriers.

Another example is a bag-type reaction gas suspension design, which can eliminate the support of the wire mesh frame and facilitate assembly and operation, but install gas in the center of the photosynthetic reactor. Ascending aeration pipe, this device can only produce local disturbance in the reactor, can not be fully mixed, and there are also phenomena such as uneven aeration and flow retention.

In one embodiment, the present disclosure provides a modular photosynthetic bioreactor system comprising at least one reactor, an intake line, a discharge line, a feed line, and an exhaust line.

The at least one reactor comprises a plurality of clamp sets, at least one film cylinder and at least one aeration plate, each clamp set comprising an outer ring member, a first disk body, an elastic ring and a second disk body, first The disk body has a flange, the flange is disposed around the outer side wall of the first disk body, the elastic ring has an elastic deformation force, and the second disk body has a brick-shaped top edge. The film cylinder has opposite opening ends, and an accommodating space is formed between the two open ends. The film cylinder has a pair of inner surfaces and an outer surface, and each of the open ends is provided with a clamp group, the first tray The elastic ring and the second disc system are disposed in the film cylinder, the outer ring member is sleeved outside the film cylinder, the elastic ring sleeve is sleeved on the outside of the first disc body, and the second disc system is sleeved on the first disc body. Externally, the top edge of the masonry is embedded between the elastic ring and the outer side wall of the first disc body, and the elastic ring is pushed toward the inner surface of the film cylinder by the masonry top edge, and the film cylinder is pressed against the outer ring member and elastic Between the rings; an aeration plate is disposed on a surface of the first disk body of the reactor facing the accommodating space.

An intake line, a discharge line, a feed line and an exhaust line are connected to the reactor, and the intake line is used to feed the mixed gas into the reactor, and the discharge line is used to provide a reaction. The reaction product in the reactor is discharged from the reactor, and the feed pipeline is used to input the reaction object into the reactor, and the exhaust pipeline is used to provide The reaction gas in the reactor is discharged from the reactor.

In one embodiment, the present disclosure further provides an algae cultivation method comprising three main procedures of performing a sterilization procedure, a breeding procedure, and a harvesting procedure. When the disinfection process is performed, the water, air and hypochlorous acid solution are injected into at least one reactor of a modular photosynthetic bioreactor system for sterilization, and then the sodium thiosulfate solution is injected. In the reactor, the residual chlorine in the reactor is neutralized; when the culture process is carried out, the medium and the algae solution are injected into the reactor for completing the disinfection process, and the microalgae is grown to a stable state. In order to promote the growth of the microalgae; when the harvesting process is carried out, the microalgae in the reactor is harvested after the period of growth of the microalgae is completed.

In one embodiment, the present disclosure further provides a clamp set including an outer ring member, a first disk body, an elastic ring, and a second disk body. The first disk body has a flange, and the flange is surrounded. The outer ring is disposed on the outer side wall of the first disk body, the elastic ring has an elastic deformation force, and the second disk body has a brick-shaped top edge.

In order to enable your review committee to have a better understanding and recognition of the structural purpose and efficacy of this disclosure, please refer to the detailed description of the illustration as follows.

The technical means and efficacy of the present disclosure for achieving the purpose will be described below with reference to the accompanying drawings, and the embodiments listed in the following drawings are only for the purpose of explanation, and are to be understood by the reviewing committee, but the technical means of the present invention are not Limited to the listed figures.

Referring to the embodiment shown in the first figure, the modular photosynthetic bioreactor system 100 comprises a multi-array reactor 10, an intake line 20, and a discharge tube. Road 30, a feed line 40, and an exhaust line 50.

In the present embodiment, five sets of reactors 10 are provided, but the number thereof is designed to be increased or decreased according to actual needs, and is not limited to the five groups shown in the figure. Each set of reactors 10 is comprised of a film cylinder 11 and two sets of clamps 12A, 12B. The film cylinder 11 is made of a transparent soft plastic material, and the thickness of the film cylinder 11 is set according to actual needs. The film cylinder 11 has an elongated cylindrical shape and has opposite opening ends 111 and 112, and an accommodation space 113 is formed between the two open ends 111 and 112. A clamp group 12A, 12B is respectively disposed at each of the open ends 111, 112. The film cylinder 11 is vertically disposed in the longitudinal direction thereof. Since the film cylinder 11 is made of a soft material, it must be supported by a rigid structure, as shown in the figure, at one of the open ends 112 of the film cylinder 11 (shown in the first figure). The clamp group 12B of the top end of the film cylinder 11 is suspended from a bracket 61 by a spreader 60, and is disposed at the other open end 111 of the film cylinder member 11 (the bottom end of the film cylinder member 11 shown in the first figure). The clamp set 12A can be supported by a platform 62 so that the reactor 10 can be stably placed between the bracket 61 and the platform 62.

An intake line 20, a discharge line 30, a feed line 40, and an exhaust line 50 are connected to each reactor 10. The intake line 20 is for introducing a mixed gas into the reactor 10, and a valve 21 is provided in the intake line 20, and by controlling the valve 21, whether or not the mixed gas flows into each of the reactors 10 can be controlled. The discharge line 30 is for providing the reaction product in the reactor 10 to discharge the reactor 10. The discharge line 30 is provided with a total valve 31 and a plurality of liquid level control valves 32A to 32D, which are connected to each reactor 10. A valve 33-37 is respectively disposed on the path of the discharge line 30. By controlling the valves 33-37, whether the reaction product in the reactor 10 flows out of the reactor 10 can be controlled, and the valves 33-37 can be designed as a linked valve mode. For example, the link 38 is turned on or off synchronously to Pneumatic, electric, mechanical operation. The feed line 40 is used to input the reaction object into the reactor 10, and a total valve 41 is disposed in the feed line 40, and a valve 42 is respectively disposed on the path of each of the reactors 10 connected to the feed line 40. ~46, by controlling the valves 42-46, it is possible to control whether or not the reaction object is input into the reactor 10. The exhaust line 50 is for providing the reaction gas discharge reactor 10 in the reactor 10, and a total valve 51 is disposed in the exhaust line 50, and the path of each of the reactors 10 connected to the exhaust line 50 is respectively A valve 52-56 is provided. By controlling the valves 52-56, it is possible to control whether the reactor body in the reactor 10 flows out of the reactor 10, and the exhaust line 50 is connected to the intake line 20 in the exhaust line. A valve 22 is disposed between the 50 and the intake line 20, and by controlling the valve 22, whether the mixed gas can be input into the reactor 10 via the exhaust line 50, that is, the exhaust line 50 and the intake pipe are controlled. The road 20 system can block the ground connection. The level of the clamp group 12A provided with the intake line 20 and the discharge line 30 is lower than the level of the clamp group 12B provided with the exhaust line 50 and the feed line 40.

Referring to the second to fourth figures, the structure of the clamp organization embodiment of the present disclosure will be described. The clamp unit 12 is mainly composed of an outer ring member 121, a first disk body 122, an elastic ring 123 and a second disk body 124. In this embodiment, the outer ring member 121 and the first disk body 122 are formed. The elastic ring 123 and the second disk body 124 are all circular. The outer ring member 121 has an inner ring wall 1211, and the inner ring wall 1211 is provided with an annular groove 1212. The first disk body 122 has a flange 1221. The flange 1221 is disposed around the outer wall 1222 of the first disk body 122. The first disk body 122 is provided with a plurality of penetrating portions, and the plurality of penetrating portions include a first penetrating portion. 1223, a second through portion 1224 and a third through portion 1225. Elastic ring 123 is made of elastic rubber material The quality of the elastic ring 123 has the ability to be deformed by force. The second disk body 124 has a masonry top edge 1241. The masonry top edge 1241 is a ring-shaped annular structure having a tapered shape. The second disk body 124 has a bottom edge 1242. The bottom edge 1242 surrounds the second body 124. The vacant portion 1243.

Referring to the fifth and sixth figures, the film cylinder 11 has a relatively inner surface 114 and an outer surface 115. The first disk 122, the elastic ring 123 and the second disk 124 are disposed on the film cylinder. 11 , the outer ring member 121 is sleeved on the outer side of the film cylinder 11 , the elastic ring 123 is sleeved on the outer side wall 1222 of the first disk body 122 and abuts against the bottom of the flange 1221 , and then the second disk body 124 is sleeved. The outer edge of the first disk body 122 is disposed at one end of the tapered shape and is embedded between the elastic ring 123 and the outer side wall 1222 of the first disk body 122. The position of the elastic ring 123 is connected to the outer ring member. The position of the annular groove 1212 of 121 corresponds to a position in which the masonry top edge 1241 has a pointed shape, and the one end of the masonry top edge 1241 can push the elastic ring 123 into the film cylinder 11 with respect to one end of the tapered end. The surface 114, and the elastic ring 123 is embedded in the annular groove 1212, so that the film cylinder 11 can be forced between the outer ring member 121 and the elastic ring 123.

Referring to the second and sixth figures, a gap d1 is formed between the first disk 122 and the second disk 124 opposite to the accommodating space 113 facing the film cylinder 11 to the second disk. A plurality of adjusting bolts 1244 are disposed on a surface of the first disk body 122. One end of each of the adjusting bolts 1244 extends through the screw hole 1245 of the bottom edge 1242 of the second disk body 124 and abuts against the first disk body. 122 faces one side of the second disk body 124. The spacing d1 between the first disk body 122 and the second disk body 124 can be adjusted by rotating the adjusting bolt 1244 to accommodate the film cylinders 11 of different thicknesses.

Referring to the first and second figures, the main structure of the clamp set 12A, 12B shown in the first figure is the same as that of the clamp set 12 shown in the second figure, and is clamped to the film by the clamp sets 12A, 12B. The two open ends 111 and 112 of the tubular member 11 can close the two open ends 111 and 112. The receiving space 113 of the film cylinder 11 can only pass through the first through portion 1223 and the second through portion of the first disk 122. The 1224 and third penetrations 1225 are in communication with the external environment. The first through portion 1223 and the second through portion 1224 are respectively connected to the intake line 20, the discharge line 30, the exhaust line 50, and the feed line 40, and the third through portion 1225 is Various detection probes are connected, and the type of the detection probe may include, for example, a probe for detecting pH, pH, dissolved oxygen (DO), temperature, and the like. Wherein, the clamp group 12A disposed at the bottom of the film cylinder 11 is connected to the intake pipe 20 and provides an aeration effect, so an aeration plate is disposed in the clamp group 12A.

Referring to the first, seventh and eighth figures, the first disk body 122A of the clamp group 12A is provided with the aeration facing the accommodating space 113 of the film cylinder 11 (shown in the first figure). The plate 125 and the aeration plate 125 are made of a rubber material, and a plurality of air holes having a hole diameter of less than 1 mm are distributed. The aeration plate 125 is fixed to the first disk body 122A by a fixing ring 126, a fixing screw 127 and the like. The road 20 is connected to the first penetration portion 1223A, and the discharge line 30 is connected to the second penetration portion 1224A. The third penetration portion 1225A does not function with respect to the clamp group 12A, and the third penetration portion 1225A can be closed. When the mixed gas enters the second disk body 124A from the intake pipe 20 and then enters the first disk body 122A and the aeration plate 125 upward, microbubbles can be generated, and when the air bubbles rise, the surrounding algae liquid can be moved up and down and left and right, and By a large area of aeration, increasing the contact between gas and liquid, the algae liquid has a good mixing effect and improves the flow retention. The algae effectively flow and circulate, fully absorb outdoor sunlight or artificial light source, and increase the growth rate of microalgae.

Referring to the first and ninth diagrams, since the clamp group 12B does not provide an aeration effect, it is not necessary to provide an aeration plate, and the first penetration portion 1223B provided in the first disk body 122B can be closed. The second penetration portion 1224B and the third penetration portion 1225B are connected to the feed line 40 and the exhaust line 50, respectively.

Please refer to the first and the tenth drawings, and illustrate a process 200 for applying the algae culture method of the modular photosynthetic bioreactor system of the present disclosure, which mainly comprises: step 202: performing a disinfection process; and step 204: performing one Breeding procedure; Step 206: Perform a harvesting procedure.

When the sterilization procedure (step 202) is performed, water, air, and hypochlorous acid solution are injected into the reactor 10 of the modular photosynthetic bioreactor system 100 via a feed line 40 for sterilization. For example, 95% of the seawater of the accommodating space 113 is injected from the feed line 40, air is introduced into the reactor 10 from the intake line 20, and a hypochlorous acid solution is introduced into the reactor 10 from the feed line 40. In the middle, disinfection is carried out for 24 hours. The sodium thiosulfate solution is then injected into the reactor 10 via a feed line 40 to neutralize the residual chlorine in the reactor 10. After the process of neutralizing the residual chlorine is completed, 10-20% of the seawater of the accommodating space 113 is excluded. During the sterilization process, the total valve 41 of the feed line 40, the valves 42-46, and the valve 21 of the intake line 20 are all open, and the other valves are closed.

The culture program (step 204) is to feed the medium through the feed line 40 and The algae solution is injected into the reactor 10 where the sterilization process has been completed, and waits for the microalgae to grow to a stable period to promote the growth of the microalgae. The medium and the algae are made up of water, salt, organic matter, nitrogen, phosphorus and algae. When the feed reaches approximately 95% of the accommodating space 113, the feed is stopped to perform the culture process and provide light and carbon dioxide to promote the growth of the microalgae. During the breeding period, the growth curve and the growth environment parameters were sampled from the photosynthetic reactor. After several days of cultivation, the period of growth of the microalgae was finished (that is, the microalgae stopped growing), and then harvested in vivo (step 206). During the process of injecting the culture medium and the algae liquid, the total valve 41 and the valves 42 to 46 of the feed line 40 are open, and the other valves are closed. During the process of the aquaculture procedure, except for the valve 21 of the intake line 20 and the total valve 51 of the exhaust line 50, valves 52-56, the other valves are closed.

The harvesting program (step 206) recovers the microalgae in the reactor 10 after the period of growth of the microalgae. First, the coagulant is injected into the reactor 10 via the feed line 40, and the microalgae grown by the algae are concentrated and precipitated in the reactor 10, and then the agglomerated concentrated microalgae are collected by the discharge line 30. . The total valve 31 and the valves 33-37 of the discharge line 30 are opened, and the liquid level control valves 32A to 32D are selectively opened according to the recovery amount, and at the same time, the total valve 51 of the exhaust line 50 is closed, and the intake pipe is closed. The valve 22 of the road 20 is opened, and gas can be introduced into the reactor 10 from the top end of the reactor 10 by the intake line 20 to increase the gas phase pressure in the reactor 10 to accelerate the discharge of the microalgae in each reactor 10. 10, and discharged from the discharge line 30, and the microalgae is selected to be collected by the liquid level control valves 32A to 32D, and the supernatant liquid of the reactor 10 is removed. For semi-continuous breeding, after the stable growth period, select the liquid level control valve level control valve 32A~32D to achieve stable recovery. Liquid level. When the step of collecting the agglomerated concentrated microalgae is performed, it is simultaneously observed whether the water level in the reactor 10 drops to a preset water level, and when the water level in the reactor 10 drops to the preset water level, the intake valve is closed. 22, the total valve 51 is opened to stop the introduction of gas into the reactor 10, while the discharge line 30 is closed to stop collecting the agglomerated concentrated microalgae, and then the fresh medium is introduced into the reactor 10 through the feed line 40, The next round of farming can be carried out.

The present invention provides a low-cost, modular, and easy-to-operate microalgae photosynthetic reactor by clamping the clamps at both ends of the film cylinder made of plastic film, and simultaneously matching the intake pipe and the discharge pipe. The connection between the feed line and the exhaust line can be systematically operated during feeding, inoculation, harvesting and cleaning after the end of the culture. By using the modular photosynthetic bioreactor system of the present disclosure to culture algae, batch and semi-continuous farming strategies can be adopted, and in semi-continuous farming, coagulation and harvesting methods can be combined to reduce the harvesting volume and long-term. to cultivate.

However, the above description is only for the embodiments of the present disclosure, and the scope of the disclosure is not limited thereto. That is to say, the average changes and modifications made by the applicants in accordance with the scope of the application for patents should still fall within the scope of the disclosure of this patent. I would like to ask your review committee to give a clear understanding and pray for the best.

100‧‧‧Modular Photosynthetic Bioreactor System

10‧‧‧Reactor

11‧‧‧film cylinder

111, 112‧‧‧ open end

113‧‧‧ accommodating space

114‧‧‧ inner surface

115‧‧‧ outer surface

12, 12A, 12B‧‧ ‧ fixture group

121‧‧‧Outer ring

1211‧‧‧ inside ring wall

1212‧‧‧ annular groove

122, 122A, 122B‧‧‧ first body

1221‧‧‧Flange

1222‧‧‧Outer side wall

1223, 1223A, 1223B‧‧‧ first penetration

1224, 1224A, 1224B‧‧‧ second penetration

1225, 1225A, 1225B‧‧‧ third penetration

123‧‧‧Flexible ring

124‧‧‧Second disk

1241‧‧‧Mask top edge

1242‧‧‧ bottom edge

1243‧‧‧The Department

1244‧‧‧Adjustment bolt

1245‧‧‧ screw hole

125‧‧‧Aeration board

126‧‧‧Fixed ring

127‧‧‧ fixing screws

20‧‧‧Intake line

21‧‧‧ Valves for the intake line

22‧‧‧Valve between exhaust line and intake line

30‧‧‧Drainage line

31‧‧‧Main valve of the discharge line

32A~32D‧‧‧ Liquid level control valve

33~37‧‧‧Drainage valve

38‧‧‧ Connecting rod

40‧‧‧feed line

41‧‧‧Main valve of the feed line

42~46‧‧‧ Valves for the feed line

50‧‧‧Exhaust line

51‧‧‧Main valve of the exhaust line

52~56‧‧‧ Valves for exhaust lines

60‧‧‧ Spreader

61‧‧‧ bracket

62‧‧‧ platform

200‧‧‧ Process

202~206‧‧‧Steps

D1‧‧‧ spacing

The first figure is a schematic diagram of an embodiment of a modular photosynthetic bioreactor system.

The second figure is a schematic structural view of an embodiment of the present invention.

The third figure is the outer ring member, the first disc body, and the elastic ring of the second embodiment. And a schematic diagram of the cross-sectional decomposition structure of the second disk body.

The fourth figure is an enlarged schematic view of the structure of the A portion of the third figure.

The fifth figure is a schematic cross-sectional structural diagram of the outer ring member, the first disk body, the elastic ring and the second disk body in the second embodiment.

The sixth figure is an enlarged schematic view of the structure of the B part of the fifth figure.

The seventh figure is a schematic diagram of the exploded structure of the fixture set combined with the aeration plate.

The eighth figure is a schematic diagram of the combined structure of the seventh embodiment.

The ninth drawing is a schematic view showing the three-dimensional structure of the jig set at the top of the reactor.

The tenth figure is a flow chart showing the steps of an embodiment of an algae culture method.

100‧‧‧Modular Photosynthetic Bioreactor System

10‧‧‧Reactor

11‧‧‧film cylinder

111, 112‧‧‧ open end

113‧‧‧ accommodating space

12A, 12B‧‧ ‧ fixture group

20‧‧‧Intake line

21‧‧‧ Valves for the intake line

22‧‧‧Valve between exhaust line and intake line

30‧‧‧Drainage line

31‧‧‧Main valve of the discharge line

32A~32D‧‧‧ Liquid level control valve

33~37‧‧‧Drainage valve

38‧‧‧ Connecting rod

40‧‧‧feed line

41‧‧‧Main valve of the feed line

42~46‧‧‧ Valves for the feed line

50‧‧‧Exhaust line

51‧‧‧Main valve of the exhaust line

52~56‧‧‧ Valves for exhaust lines

60‧‧‧ Spreader

61‧‧‧ bracket

62‧‧‧ platform

Claims (12)

A modular photosynthetic bioreactor system comprising: at least one reactor comprising: a plurality of clamp sets, each of the clamp sets comprising: an outer ring member; a first disk body having a flange, the a flange is disposed around the outer side wall of the first disc body; an elastic ring having an elastic deformation force; a second disc body having a masonry top edge; at least one film cylinder member having two opposite sides An open end, an accommodating space is formed between the two open ends, the film cylinder has a pair of inner surfaces and an outer surface, and each of the open ends is provided with the clamp set, the first disc body, the first disc body The elastic ring and the second disk system are disposed in the film cylinder, the outer ring is sleeved outside the film cylinder, the elastic ring is sleeved on the outside of the first disk, and the second disk system is sleeved Provided on the outside of the first disc body, the masonry top edge is embedded between the elastic ring and the outer side wall of the first disc body, and the elastic top ring is pushed toward the film cylinder by the masonry top edge An inner surface that urges the film cylinder between the outer ring member and the elastic ring; and at least An aeration plate, wherein the first disk body is disposed on a surface of the at least one reactor facing the accommodating space; the air inlet pipe is connected to the reactor, and the air inlet pipe is connected to the reactor Used to feed a mixed gas into the reactor; a discharge line is connected to the reactor, and the discharge line is used to provide a reaction product in the reactor to discharge the reactor; a feed line connected to the reactor, the intake line is for inputting a reaction object into the reactor; and an exhaust line is connected to the reactor, the exhaust line is connected A reaction gas for providing the reactor is discharged from the reactor. The modular photosynthetic bioreactor system of claim 1, wherein the first disk body is provided with a plurality of penetrating portions, the plurality of penetrating portions including a first penetrating portion, a second penetrating portion, and a a third penetration portion, the intake line is connected to the first through portion of one of the set of the reactor, and the discharge line is connected to the set of the clamp set provided with the intake line a second penetration portion, the feed line is connected to the third through portion of the reactor relative to another set of the clamp connected to the intake line, the exhaust line is connected to the feed tube The second through portion of the clamp group of the road, the exhaust line is connected to the intake line in a blocking manner, so that the intake line can input the mixed gas into the reaction through the exhaust line Inside the device. The modular photosynthetic bioreactor system of claim 1, wherein the outer ring member has an inner annular wall, and the inner annular wall is provided with an annular groove, and the annular groove is positioned The position of the elastic ring corresponds, and the elastic ring is embedded in the annular groove. The modular photosynthetic bioreactor system of claim 1, wherein the first disk has a spacing from the surface facing the receiving space and the second disk. The second disk body is provided with a plurality of adjusting bolts on a surface of the first disk body, and one end of each of the adjusting bolts penetrates the second disk body and abuts against the first disk body toward the second disk body On one side, the first disk body can be adjusted by rotating the adjusting bolt The spacing between the second disks. The modular photosynthetic bioreactor system of claim 1, wherein the intake line, the exhaust line, the discharge line, and the feed line are provided with at least one control valve And for controlling the opening and closing of the intake line, the exhaust line, the discharge line, and the feed line. The modular photosynthetic bioreactor system of claim 1, wherein the level of the clamp set provided with the intake line and the discharge line is lower than the arrangement of the row The level of the gas line and the set of clamps of the feed line. An algae culture method comprising: performing a disinfection process for injecting water, air and hypochlorous acid solution into at least one reactor of a modular photosynthetic bioreactor system for sterilization and sterilization; a sodium thiosulfate solution is injected into the reactor to neutralize the residual chlorine in the reactor; a culture process is carried out by injecting the culture medium and the algae solution into the reactor for completing the sterilization process, and waiting for the microalgae Growing to a stable period to promote microalgae growth; and performing a harvesting process for harvesting the microalgae in the reactor after the period of growth of the microalgae. The method for breeding algae according to claim 7, wherein the breeding program injects water, salt, organic matter, nitrogen, phosphorus and algae into the reactor, and provides light and carbon dioxide to promote the algae species. The microalgae that it has grow. The algae culture method according to claim 7, wherein the harvesting process is to inject a coagulant into the reactor to grow the algae species. The microalgae are concentrated and precipitated in the reactor, and the reactor is connected to a discharge line through which the agglomerated concentrated microalgae are collected. The algae culture method according to claim 9, wherein the at least one reactor is connected to an exhaust line, and one end of the reactor connected to the discharge line is at a first height, and the reactor is connected. One end of the exhaust line is located at a second height, the second height is higher than the first height, and the step of collecting the agglomerated concentrated algae is to input gas into the reactor from the exhaust line In order to increase the gas phase pressure in the reactor to accelerate the agglomerated concentrated microalgae to discharge the reactor. The method for breeding algae according to claim 10, wherein when the step of collecting the agglomerated concentrated microalgae is performed, it is simultaneously observed whether the water level in the reactor drops to a predetermined water level in the reactor. When the water level drops to the preset water level, the exhaust line is closed to stop the gas from being input into the reactor, and the discharge line is closed to stop collecting the agglomerated concentrated microalgae, and then through a feed. The tubing enters the fresh medium into the reactor and proceeds to the next round of farming. A clamp set comprising: an outer ring member; a first disk body having a flange circumferentially disposed on an outer side wall of the first disk body; an elastic ring having an elastic deformation force; A second disk body having a masonry top edge.