TW201400094A - Method for preparing artificial skin dressing with antibacterial and hemostatic functions - Google Patents

Abstract

The invention relates to a method for preparing an artificial skin dressing with antibacterial and hemostatic functions. Firstly, a first excipient, a nanosilver porous composite material and a first polysaccharide are mixed, heated and stirred until the first polysaccharide is completely dissolved, to form a substrate. Secondly, the substrate is pressed and coated on a soft plastic board by means of a filter screen as well as filtered out the moisture therein to eventually form a dry substrate film with a certain thickness. Continuously, a second excipient, a natural colloid and a second polysaccharide are mixed, heated and stirred until the second polysaccharide is completely dissolved, to form a shell material. Then the shell material is pressed and coated on the surface of the substrate film by means of a filter screen as well as filtered out the moisture therein. Accordingly, it can not only inhibit the growing and breeding of bacteria and microorganisms on the artificial skin dressing but also promote a blood coagulation reaction for acceleration of wound healing.

Description

Antibacterial hemostasis artificial skin dressing preparation method

The invention relates to a method for preparing an antibacterial hemostatic artificial skin dressing, in particular to a method capable of simultaneously inhibiting the growth and reproduction of bacteria and microorganisms on an artificial skin dressing and promoting blood coagulation reaction.

According to the skin, the skin is the largest tissue structure of the human body. It not only has the function of regulating body temperature and regulating the water balance in the body, but also the first line of defense against the invasion of external pathogens. When the skin is injured in a large area, it may cause imbalance of body function. Organs are seriously dysfunctional and even die; in general, skin wounds are still in contact with clothing or the outside world after being treated with drugs. If they are accidentally caught, they may be damaged by dust or bacteria in the air. In this case, in order to avoid exposure of the wound to the air, it is common to cover or wrap a treated artificial skin dressing on the treated wound for isolation.

At present, the treatment of external trauma of the human body, such as cuts, abrasions, surgical incisions, ulcers or burns, is usually required to cover or wrap the sterilized artificial skin dressings at the wounds to prevent external bacteria. Infection; however, the artificial skin dressings used in general medical institutions need to be changed frequently. Most of them are cotton pads made by sterilizing cotton between gauze or two layers of gauze. When used, they are covered or wrapped. On the wound, although it has the effect of absorbing the exudate of the wound for the shallow wound, it still has the exudation oozing to corrode the skin around the wound, thus causing infection, or the absorbed exudate is easy to volatilize and the gauze becomes hard. Loss of wounds and other defects, there is a need to find a solution.

In order to improve the problem that the dressing is easily infected by microorganisms or bacteria, inorganic silver series antibacterial agents (for example, silver ions) have been used as raw materials for antibacterial dressings to inhibit the growth and reproduction of bacteria and reduce wound infection. Opportunity; the principle is that silver ions can act on the cell wall of bacteria to make the cell wall unable to replicate, can also destroy the cellular respiratory chain, block the energy supply of cells, and even directly act on DNA or ribonucleic acid (RNA). Characteristics that make cells unable to multiply; however, silver-containing dressings are difficult to control the release of silver ions, and it is generally believed that silver ions can cause DNA to lose the ability to replicate proteins and also form bonds with certain functional groups on proteins. The knot, thus causing damage to the protein, so when the release amount of silver ions reaches a certain level, there will be doubts that cause harm to human health; in addition, if the wound cannot heal, it is also likely to cause bacterial or viral infection, which causes serious Consequences such as cellulitis or fatal sepsis; in order to accelerate the wound Together, promote blood coagulation reaction, to reduce wound infection, the efficacy of artificial skin in the dressing also need to be improved.

Nowadays, the inventor is still in the spirit of tirelessness in view of the above-mentioned existing artificial skin dressing related products, and is supported by its rich professional knowledge and years of practical experience. Improvements have been made, and the present invention has been developed based on this.

The main object of the present invention is to provide an antibacterial hemostatic artificial skin dressing preparation method which can simultaneously inhibit the growth and reproduction of bacteria and microorganisms on artificial skin dressings and promote the blood coagulation reaction.

In order to achieve the above-mentioned purposes, the inventors have developed the following implementation techniques, first mixing a first excipient, a nano-silver porous composite, and a first polysaccharide, heating and stirring until the first The saccharide is completely dissolved and becomes the substrate of the artificial skin dressing; then, the substrate is uniformly screen printed on the plastic soft board (for example, PE board) by the screen printing method, and the moisture is completely removed to form a a substrate film that is dried and has a specific thickness; then, a second excipient, a natural colloid, and a second polysaccharide are mixed, heated and continuously stirred until the second polysaccharide is completely dissolved, thereby becoming an artificial skin dressing Finally, the shell material is formed on the surface of the substrate film and heated to remove all moisture to form an antibacterial hemostatic artificial skin dressing having a two-layer film in which the substrate and the shell material are mixed.

The antibacterial hemostatic artificial skin dressing preparation method as described above, wherein the first excipient and the second excipient are respectively selected from the group consisting of starch, mannitol, lactose, sorbitol and polyethylene glycol; preferably The first and second excipients are all selected from the group consisting of sorbitol.

The antibacterial hemostatic artificial skin dressing preparation method as described above, wherein the first polysaccharide system has a functional group of a carboxyl group; preferably, the first polysaccharide system is sodium alginate.

The antibacterial hemostatic artificial skin dressing preparation method as described above, wherein the second polysaccharide system has an amine group functional group; preferably, the second polysaccharide system is chitosan.

The antibacterial hemostatic artificial skin dressing preparation method as described above, wherein the natural colloid may be selected from the group consisting of algin, collagen, gelatin and pectin.

The antibacterial hemostatic artificial skin dressing preparation method as described above, wherein when preparing the artificial skin dressing substrate, a moisturizing agent may be further added, the moisturizing agent is selected from the group consisting of sorbitol, hyaluronic acid stock solution, vegetable glycerin, vitamin B5, and allantoin capsule. a group of elements, urea, and mixtures thereof; thereby maintaining wound surface moisture, thereby reducing wound pain and accelerating wound healing.

The antibacterial hemostatic artificial skin dressing preparation method as described above, wherein when the artificial skin dressing substrate is prepared, a thickener may be further added, and the thickener may be selected from the group consisting of alginic acid, polyglutamic acid, polyvinyl alcohol, A group of chitosan and a mixture thereof.

Thereby, the antibacterial substrate formed by mixing the sodium alginate and the nano silver porous composite material, and the hemostatic shell material formed by mixing the gelatin and the chitosan make the invention have the effects of antibacterial and hemostasis simultaneously. It can inhibit the growth and reproduction of bacteria and microorganisms on the dressing, reduce the chance of wound infection, and promote blood coagulation reaction to accelerate the healing of wounds, thus reducing the chance of wound infection.

In addition, the invention utilizes the screen printing method to apply the screen printing method, and uniformly prints the substrate of the artificial skin dressing on the plastic soft board to form a substrate film having an antibacterial repair function, so that the dressing is thin and soft. In the wound coverage, the nano-silver porous composite material is used to isolate the external bacteria, and the sodium alginate is used to promote the regeneration of wound new tissue, to achieve anti-infection and promote wound healing speed, thereby reducing the number of dressing changes.

The object of the present invention and its structural and functional advantages will be explained in conjunction with the specific embodiments according to the structure shown in the following drawings, so that the reviewing committee can have a more in-depth and specific understanding of the present invention.
First, please refer to the first figure, which is a flow chart of a preferred embodiment of a method for preparing an antibacterial hemostatic artificial skin dressing of the present invention, which comprises the following steps:

Step 1 (S1): mixing a first excipient, a nano-silver porous composite and a first polysaccharide, heating and stirring until the first polysaccharide is completely dissolved, and becomes the basis of the artificial skin dressing. Wherein the first polysaccharide system has a carboxyl group functional group, preferably, the first polysaccharide system is alginate, wherein the alginic acid is insoluble from brown seaweed Colloidal acid, a group of polymers containing different proportions of D-mannuronic acid and L-guluronic acid, such as α-1, 4 and β -1,4 bond into a linear polymer, molecular weight of about 240,000, sodium salt is soluble in water, calcium salt is insoluble in water; note that the preparation method of the above nano silver porous composite is suitable for the application The person applied for the invention patent of the Republic of China on April 16, 101 (application number) No. 101113509), the content of the "preparation method of porous antibacterial composite material with nano silver" is used as a reference material in the present case;

Step 2 (S2): uniformly coating the substrate on the plastic soft board by screen coating, and completely removing the water to form a dry and specific thickness substrate film; wherein, the screen version The pressure coating screen printing method is to apply the substrate to a special size filter screen, and then screen printing on a plastic soft board (for example, a PE board) under appropriate pressure;

Step 3 (S3): mixing a second excipient, a natural colloid, and a second polysaccharide, heating and stirring until the second polysaccharide is completely dissolved, and becomes a shell material of the artificial skin dressing; The second polysaccharide system has a functional group of an amine. Preferably, the second polysaccharide system is chitosan; further, the natural colloid may be selected from the group consisting of aligning and collagen ( a population consisting of collagen, gelatin, and pectin, preferably, the natural gum system is selected from the group consisting of gelatin;

Step 4 (S4): forming a shell material on the surface of the substrate film and heating to remove all moisture to form an antibacterial hemostatic artificial skin dressing having a mixed double layer film of the substrate and the shell material.

Wherein, the first excipient and the second excipient are respectively selected from the group consisting of starch, mannitol, lactose, sorbitol, and polyethylene glycol (PEG). Preferably, the first and second excipients are all selected from the group consisting of sorbitol.

In addition, in the above step (S1), a moisturizing agent may be further added, and the moisturizing agent is selected from the group consisting of sorbitol, hyaluronic acid stock solution, vegetable glycerin, vitamin B5, allantoin, urea ( Urea) and the group of its mixture.

Further, when the first step (S1) is performed, a thickener may be further added, and the thickener may be selected from the group consisting of alginate, poly(gamma-glutamic acid), A group consisting of polyvinyl alcohol, chitosan, and mixtures thereof.

In addition, the scope of the invention may be further demonstrated by the following practical embodiments, but is not intended to limit the scope of the invention in any way:

First, 20 g of silver nitrate crystals were dissolved in 80 g of deionized water and placed in a 20% by weight silver nitrate solution; then 37.8 mL of silver nitrate solution was dissolved in 500 mL of deionized water to prepare a solution A; then, 10 g was obtained. The gelatin is dissolved in 300 mL of deionized water, and the temperature is maintained at 40 ° C. Stirring is continued to dissolve the gelatin uniformly; then, the solution A is poured and continuously stirred to prepare a solution B; 0.2 g of sodium borohydride is dissolved. In 100 mL of deionized water, and adding Solution B, the silver ions in Solution B can be reduced to silver particles to prepare Solution C, and the chemical reaction formula of the silver ions which can be reduced to silver particles is as follows:

Next, the alkali made of sodium hydroxide is dropped into the solution C, the pH of the solution C is adjusted to 6, and the temperature is maintained at 40 degrees Celsius to prepare the solution D; then, 40 g of sodium citrate is dissolved. 500mL deionized water, stirred to dissolve evenly, to obtain an alkaline solution E; 10mL of hydrochloric acid was added to 300ml of deionized water to prepare an acid solution to prepare a solution F; the solution F was quickly poured into the solution E, and rapidly stirred The pH of the solution is rapidly lowered to about 1.5 to prepare a strongly acidic solution G. Then, the solution G is slowly dropped into the alkali solution prepared by using sodium hydroxide to adjust the pH to 6 to obtain a weak acidity. Solution H; finally, the solution D is quickly poured into the solution H, and mixed to obtain a nano-silver porous composite of silver-yttria complex, that is, the silver particles can be coated on the porous cerium oxide rubber. In the structure.

Next, 8 g of deionized water and 2 g of sorbitol solution were uniformly mixed, and 0.01 g of the nano silver porous composite material was added thereto, and uniformly dispersed by ultrasonic vibration, and then 1 g of sodium alginate was added thereto, and heated to 100 ° C and continuous stirring until completely dissolved, configure a substrate containing 3000 ppm of artificial skin dressing; followed by a special size and thickness of the filter substrate to the appropriate substrate (for example, 1.5 atm) The screen is printed on a plastic soft board and heated to 80 ° C for 1 hour to completely remove the moisture to form a substrate film with antibacterial repair function; it is worth noting that the size of the mesh grid determines the thickness of the substrate film. Generally, the porosity of the mesh may be between 100 μm and 500 μm.

Then, another 2 g of deionized water and 1 g of sorbitol solution are uniformly mixed, and then 1 g of gelatin and 0.1 g of chitosan are added, and the mixture is heated to 60 ° C and continuously stirred until the chitosan is completely dissolved. As a shell material for artificial skin dressings; finally, the shell material is thermostated at 60 ° C, the shell material is coated with a special size and thickness of the screen, and screen printed under appropriate pressure (for example, 1.5 atm) The surface of the substrate film; thereby, the substrate film also has a hemostatic effect, and is heated to 80 ° C for 1 hour to remove all moisture, thereby preparing an artificial skin dressing having a hemostatic antibacterial repair function, so that the artificial skin dressing of the present invention The invention has a double-layer film mixed with a substrate and a shell material.

It can be seen from the above preparation method and implementation description of the antibacterial hemostatic artificial skin dressing that the present invention has the following advantages:

1. The antibacterial substrate formed by mixing sodium alginate and nano silver porous composite material, and the hemostatic shell material formed by mixing gelatin and chitosan make the invention have antibacterial and hemostasis effects simultaneously. It can inhibit the growth and reproduction of bacteria and microorganisms on the dressing, reduce the chance of wound infection, and promote blood coagulation reaction to accelerate the healing of wounds, thus reducing the chance of wound infection.
2. The invention utilizes the method of screen coating by pressure screen printing to uniformly form the substrate of the artificial skin dressing on the plastic soft board to form a substrate film with antibacterial repair function, so that the dressing is thin and soft and suitable for wound covering. Then, the nano-silver porous composite material is used to isolate the external bacteria, and the sodium alginate is used to promote the regeneration of the wound new tissue, to achieve the anti-infection and promote the wound healing speed, thereby reducing the number of dressing changes, and the screen plate is pressurized. The method of coating the screen printing is different from the conventional dipping method, the spraying method or the plate scraping method, etc., so that the present invention can accurately control the thickness of the film.
3. The preparation method of the antibacterial hemostasis artificial skin dressing of the invention can maintain the wound surface moisture by the addition of the moisturizing agent, thereby reducing the wound pain and accelerating the healing of the wound surface.

In summary, the preparation method of the antibacterial hemostasis artificial skin dressing of the present invention can achieve the intended use effect by the above disclosed embodiments, and the present invention has not been disclosed before the application, and has completely complied with the patent law. Regulations and requirements.爰Issuing an application for a patent for invention in accordance with the law, and asking for a review, and granting a patent, is truly sensible.

The illustrations and descriptions of the present invention are merely preferred embodiments of the present invention, and are not intended to limit the scope of the present invention; those skilled in the art, which are characterized by the scope of the present invention, Equivalent variations or modifications are considered to be within the scope of the design of the invention.

(S1). . . step one

(S2). . . Step two

(S3). . . Step three

(S4). . . Step four

First Figure: Flow chart of the preparation method of the present invention

(S1). . . step one

(S2). . . Step two

(S3). . . Step three

(S4). . . Step four

Claims (10)

The preparation method of the antibacterial hemostasis artificial skin dressing comprises the following steps:
Step 1: mixing a first excipient, a nano-silver porous composite and a first polysaccharide, heating and stirring until the first polysaccharide is completely dissolved, and becomes a substrate of the artificial skin dressing;
Step 2: uniformly coating the substrate on the plastic soft board by screen coating, and completely removing the water to form a dry and specific thickness substrate film;
Step 3: mixing a second excipient, a natural colloid, and a second polysaccharide, heating and stirring until the second polysaccharide is completely dissolved to become a shell material of the artificial skin dressing; and step 4: The shell material is uniformly screen printed on the surface of the base film by screen coating, and heated to remove all moisture to form an antibacterial hemostatic artificial skin dressing. The method for preparing an antibacterial hemostatic artificial skin dressing according to claim 1, wherein the first excipient and the second excipient are respectively selected from the group consisting of starch, mannitol, lactose, and sorbus. A group of sorbitol and polyethylene glycol (PEG). The method for preparing an antibacterial hemostatic artificial skin dressing according to claim 2, wherein the first excipient and the second excipient are both selected from sorbitol. The method for preparing an antibacterial hemostatic artificial skin dressing according to claim 1, wherein the first polysaccharide system has a carboxyl group functional group. The method for preparing an antibacterial hemostatic artificial skin dressing according to claim 4, wherein the first polysaccharide system is alginate. The method for preparing an antibacterial hemostatic artificial skin dressing according to claim 1, wherein the second polysaccharide system has an amine functional group. The method for preparing an antibacterial hemostatic artificial skin dressing according to claim 6, wherein the second polysaccharide system is chitosan. The method for preparing an antibacterial hemostatic artificial skin dressing according to claim 1, wherein the natural gum system is selected from the group consisting of align, collagen, gelatin, and pectin. Ethnic group. The method for preparing an antibacterial hemostatic artificial skin dressing according to the first aspect of the invention, wherein the moisturizing agent is further selected from the group consisting of sorbitol, hyaluronic acid stock solution, vegetable glycerin, A group of vitamin B5, allantoin, urea, and mixtures thereof. The method for preparing an antibacterial hemostatic artificial skin dressing according to claim 1, wherein in the step 1, a thickener is further added, which is selected from the group consisting of alginic acid and polyglutamic acid ( A group consisting of poly(γ-glutamic acid), polyvinyl alcohol, chitosan, and the like.