CN107496973B - Chitosan sponge pad band-aid capable of rapidly stopping bleeding and preparation method thereof - Google Patents
Chitosan sponge pad band-aid capable of rapidly stopping bleeding and preparation method thereof Download PDFInfo
- Publication number
- CN107496973B CN107496973B CN201710902981.2A CN201710902981A CN107496973B CN 107496973 B CN107496973 B CN 107496973B CN 201710902981 A CN201710902981 A CN 201710902981A CN 107496973 B CN107496973 B CN 107496973B
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- Prior art keywords
- chitosan
- sponge
- quick
- sponge cushion
- cushion layer
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 229920001661 Chitosan Polymers 0.000 title claims abstract description 137
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 230000000740 bleeding effect Effects 0.000 title abstract description 17
- 230000023597 hemostasis Effects 0.000 claims abstract description 72
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- 239000008103 glucose Substances 0.000 claims abstract description 30
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 claims abstract description 28
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- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 60
- 230000002439 hemostatic effect Effects 0.000 claims description 53
- 230000002195 synergetic effect Effects 0.000 claims description 36
- 238000001035 drying Methods 0.000 claims description 26
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- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 description 2
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- OCKGFTQIICXDQW-ZEQRLZLVSA-N 5-[(1r)-1-hydroxy-2-[4-[(2r)-2-hydroxy-2-(4-methyl-1-oxo-3h-2-benzofuran-5-yl)ethyl]piperazin-1-yl]ethyl]-4-methyl-3h-2-benzofuran-1-one Chemical compound C1=C2C(=O)OCC2=C(C)C([C@@H](O)CN2CCN(CC2)C[C@H](O)C2=CC=C3C(=O)OCC3=C2C)=C1 OCKGFTQIICXDQW-ZEQRLZLVSA-N 0.000 description 1
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- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
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- BQINXKOTJQCISL-GRCPKETISA-N keto-neuraminic acid Chemical group OC(=O)C(=O)C[C@H](O)[C@@H](N)[C@@H](O)[C@H](O)[C@H](O)CO BQINXKOTJQCISL-GRCPKETISA-N 0.000 description 1
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- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/00051—Accessories for dressings
- A61F13/00063—Accessories for dressings comprising medicaments or additives, e.g. odor control, PH control, debriding, antimicrobic
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/02—Adhesive bandages or dressings
- A61F13/0259—Adhesive bandages or dressings characterised by the release liner covering the skin adhering layer
- A61F13/0266—Adhesive bandages or dressings characterised by the release liner covering the skin adhering layer especially adapted for wound covering/occlusive dressings
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/02—Adhesive bandages or dressings
- A61F13/0276—Apparatus or processes for manufacturing adhesive dressings or bandages
- A61F13/0289—Apparatus or processes for manufacturing adhesive dressings or bandages manufacturing of adhesive dressings
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/20—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing organic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F2013/00089—Wound bandages
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/402—Anaestetics, analgesics, e.g. lidocaine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Materials Engineering (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Manufacturing & Machinery (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention belongs to the technical field of medical instruments, and particularly relates to a chitosan sponge pad band-aid capable of rapidly stopping bleeding and a preparation method thereof. The invention provides a quick hemostasis chitosan sponge cushion band-aid which comprises medical isolation paper, a sponge cushion layer and viscose cloth, wherein the sponge cushion layer is positioned in the middle section of the viscose cloth, the isolation paper is attached to the viscose cloth and the sponge cushion layer, the sponge cushion layer contains a quick hemostasis medicament, and the quick hemostasis medicament comprises the following components in parts by weight: 35-55 parts of chitosan mixture, 10-15 parts of glucose aqueous solution and 5-8 parts of mannose. The chitosan sponge cushion band-aid capable of rapidly stopping bleeding provided by the invention can rapidly and strongly stop bleeding, has the effects of resisting bacteria, relieving pain, promoting wound healing and the like, is non-toxic and non-irritant, has high use safety, and is suitable for medical rapid hemostasis, wound healing promotion and the like.
Description
Technical Field
The invention belongs to the technical field of medical instruments, and particularly relates to a chitosan sponge pad band-aid capable of rapidly stopping bleeding and a preparation method thereof.
Background
The band-aid, also called as a hemostatic plaster, is a surgical medicine most commonly used in people's life, and is usually used for emergency treatment of small wounds, thereby playing the roles of temporary hemostasis and wound surface protection. The hemostatic materials currently used in the band-aid mainly include zeolite, collagen powder, potato starch, gelatin sponge, collagen sponge, oxidized regenerated cellulose, fibrin glue, and the like, which have certain disadvantages, such as: the tissue adhesion of gelatin and collagen is poor, and both of them depend on sufficient platelets and coagulation factors for their hemostatic function; oxidized regenerated cellulose and fibrin glue may cause infection; porous zeolites and potato starch give off a large amount of heat after absorbing water in the blood, possibly causing inflammation of the wound.
The chitosan is a natural high molecular compound derived from oceans and is a natural animal dietary fiber, animal experiments prove that the chitosan has no acute or long-term toxicity, the basic unit of the chitosan is glucosamine, and the glucosamine is a substance existing in a human body, so the chitosan has good affinity with human body cells, has no rejection reaction, has good biocompatibility, can be biodegraded and has the function of stopping bleeding. The hemostatic property of chitosan is that chitosan has a certain amount of charges, and its molecules can directly connect red blood cells on the surface of a wound together to promote blood coagulation, thereby achieving the hemostatic effect. In addition, the wound dressing made of chitosan also has the functions of absorbing water and permeating oxygen, so that wound tissues under the dressing can obtain enough oxygen partial pressure, epithelial cells can crawl from the periphery, and the growth of fibroblasts is inhibited.
Chinese patent application CN104324409A discloses a novel rapid hemostatic adhesive bandage, which comprises a hemostatic layer, a microporous polytetrafluoroethylene layer and an isolation layer, wherein the microporous polytetrafluoroethylene layer is located between the hemostatic layer and the isolation layer, the hemostatic layer, the microporous polytetrafluoroethylene layer and the isolation layer are bonded by a binder, and the hemostatic layer comprises the following raw materials in parts by weight: 15-35 parts of pullulan polysaccharide, 10-25 parts of chitosan quaternary ammonium salt, 15-30 parts of succinyl chitosan, 18-30 parts of glucan, 10-15 parts of sodium hyaluronate, 3-8 parts of indigo naturalis, 3-8 parts of antibacterial agent, 3-8 parts of anti-inflammatory agent, 5-15 parts of starch and 5-10 parts of plasticizer.
The Chinese patent application CN104307027A discloses a medical chitosan rapid hemostasis composite dressing, which comprises the following raw materials in parts by weight: 15-25 parts of chitosan, 10-20 parts of glucan, 15-25 parts of hydroxypropyl chitosan, 1-5 parts of antibacterial agent, 2-8 parts of tranquilizer and antiphlogistic and 2-10 parts of mannose.
Above-mentioned novel band-aid of stanching fast and medical chitosan compound dressing that stanchs fast all can play the effect of hemostasis, nevertheless have single scheduling problem of pharmacological action, and still need further improvement in the aspect of hemostatic effect, consequently, from giving full play to chitosan hemostatic performance in the band-aid and improving aspects such as hemostatic effect and consider, still lack a chitosan foam-rubber cushion band-aid of stanching fast among the prior art.
Disclosure of Invention
In order to solve the technical problems in the prior art, the invention aims to provide a chitosan sponge pad band-aid capable of rapidly stopping bleeding and a preparation method thereof. The chitosan sponge pad band-aid for rapid hemostasis provided by the invention optimizes the composition and proportion of a chitosan mixture, improves the preparation method of the chitosan sponge pad band-aid for rapid hemostasis, can rapidly and strongly stanch bleeding, has the effects of resisting bacteria, relieving pain, promoting wound healing and the like, is non-toxic and non-irritant, has high use safety, and is suitable for the aspects of rapid hemostasis for medical use, promotion of wound healing and the like.
The technical scheme of the invention is as follows:
the utility model provides a quick hemostasis chitosan foam-rubber cushion woundplast comprises medical barrier paper, sponge bed course, viscose cloth, and the sponge bed course is located the viscose cloth middle section, and the barrier paper adheres to on viscose cloth and sponge bed course, contain quick hemostasis medicament in the sponge bed course, quick hemostasis medicament includes following component and part by mass: 35-55 parts of chitosan mixture, 10-15 parts of glucose aqueous solution and 5-8 parts of mannose.
Preferably, the quick hemostatic medicament consists of the following components in parts by weight: 48 parts of chitosan mixture, 12 parts of glucose aqueous solution and 7 parts of mannose.
Preferably, the chitosan mixture consists of chitosan with deacetylation degree of 80-90%, chitosan quaternary ammonium salt and hydroxypropyl chitosan according to the mass ratio of 5-8: 2-3: 1.
More preferably, the chitosan mixture consists of chitosan with deacetylation degree of 85%, chitosan quaternary ammonium salt and hydroxypropyl chitosan according to the mass ratio of 7:2.5: 1.
Preferably, the mass concentration of the glucose aqueous solution is 45-55%.
More preferably, the glucose aqueous solution has a mass concentration of 50%.
In addition, the invention also provides a preparation method of the chitosan sponge cushion adhesive bandage for rapid hemostasis, which comprises the following steps:
s1: taking a chitosan mixture, a glucose aqueous solution and mannose according to the specified mass part, putting the chitosan mixture, the glucose aqueous solution and the mannose into a reactor, adding an acetic acid aqueous solution with the volume concentration of 1.5-2.5%, and stirring until the chitosan mixture, the glucose aqueous solution and the mannose are completely dissolved to prepare a mixed solution;
s2: heating the mixed solution prepared in the step S1 at the constant temperature of 55-70 ℃ for 15-30min to obtain a quick hemostatic agent;
s3: dividing the quick hemostatic medicament prepared in the step S2 into a quick hemostatic medicament A and a quick hemostatic medicament B, wherein the volume ratio of the quick hemostatic medicament A to the quick hemostatic medicament B is 7-9: 2-4;
s4: soaking the sponge cushion layer into the quick hemostatic agent A prepared in the step S3, taking out after 10-25h, and drying for 2-4h at 60-70 ℃ by adopting a drying oven to obtain a synergistic sponge cushion layer A;
s5: taking out the synergistic sponge cushion layer A prepared in the step S4, uniformly spraying the quick hemostatic agent B prepared in the step S3 on the synergistic sponge cushion layer A, and drying for 0.5-2h at 60-70 ℃ by adopting a drying oven to obtain a synergistic sponge cushion layer B;
s6: and (5) sticking the synergistic sponge cushion layer B prepared in the step (S5) to the middle section of the adhesive tape, and covering the surfaces of the adhesive tape and the sponge cushion layer with isolation paper to obtain the synergistic sponge cushion layer B.
Preferably, the step S1 is to add 2 vol% acetic acid solution.
Preferably, the amount of the acetic acid aqueous solution added in step S1 is 6 to 20 times of the total mass of the chitosan mixture, the glucose aqueous solution and the mannose.
More preferably, the amount of the acetic acid aqueous solution added in step S1 is 14 times the total mass of the chitosan mixture, the glucose aqueous solution and the mannose.
Preferably, the step S2 is to heat the mixed solution prepared in the step S1 at a constant temperature of 60 ℃ for 20 min.
Preferably, the volume ratio of the rapid hemostatic agent A and the rapid hemostatic agent B in the step S3 is 8: 3.
Preferably, the sample is taken out after 16h in the step S4 and dried for 3h at 65 ℃ by using a drying oven.
Preferably, the step S5 is then dried for 1.5h at 65 ℃ using a drying oven.
The chitosan can interact with a receptor of neuraminic acid residues on the surface of cells, promote the aggregation of platelets, activate a blood coagulation system, and crosslink red blood cells to form blood clots, has an obvious film forming effect, and can accelerate wound healing and prevent massive bleeding. However, the single chitosan has poor water solubility, so that the preparation process of the single chitosan hemostatic material is complicated, and the application is greatly limited, the invention improves the chitosan hemostatic effect by introducing hydroxypropyl chitosan containing hydrophilic groups and chitosan quaternary ammonium salt to carry out physical and chemical modification on chitosan so as to increase the solubility of the chitosan, and in addition, the hydroxypropyl chitosan is non-toxic, tasteless and good in biocompatibility, has the performances of hemostasis, analgesia, bacteriostasis and the like, and can play the role of an analgesic under a certain dosage; the chitosan quaternary ammonium salt has good antibacterial property, film forming property, moisture absorption and retention property and other properties, the antibacterial property of the chitosan quaternary ammonium salt is superior to that of chitosan and other chitosan derivatives, and the chitosan quaternary ammonium salt can play a role of an antibacterial agent under a certain dosage. Therefore, the chitosan mixture is preferably prepared from chitosan, chitosan quaternary ammonium salt and hydroxypropyl chitosan according to the mass ratio of 5-8: 2-3: 1, and the chitosan mixture has strong quick hemostasis performance and excellent analgesic and antibacterial effects. Meanwhile, the invention adopts 45-55% glucose aqueous solution to accelerate wound healing.
The rapid hemostatic chitosan sponge cushion prepared by the invention has high content of the rapid hemostatic medicament in the sponge cushion layer and high surface content, so that the effect of the rapid hemostatic medicament in the sponge cushion layer is enhanced.
Compared with the prior art, the chitosan sponge cushion band-aid for rapid hemostasis provided by the invention has the following advantages:
(1) the chitosan sponge cushion band-aid capable of rapidly stopping bleeding provided by the invention can rapidly and strongly stop bleeding, is non-toxic and non-irritant, and has high use safety.
(2) The chitosan sponge cushion band-aid for rapid hemostasis provided by the invention has the effects of resisting bacteria, easing pain, relieving pain, promoting wound healing and the like.
(3) The chitosan sponge cushion band-aid for rapid hemostasis provided by the invention is simple and easy to prepare, low in preparation cost, and suitable for medical rapid hemostasis, promotion of wound healing and the like.
Detailed Description
The present invention will be further described below by way of specific embodiments, but the present invention is not limited to only the following examples.
Example 1A Chitosan sponge pad band-aid for rapid hemostasis
Quick hemostasis chitosan foam-rubber cushion woundplast comprises medical barrier paper, sponge bed course, viscose cloth, and the sponge bed course is located the viscose cloth middle section, and the barrier paper adheres to on viscose cloth and sponge bed course, contain quick hemostasis medicament in the sponge bed course, quick hemostasis medicament comprises following component and part by mass: 35 parts of chitosan mixture, 10 parts of glucose aqueous solution with the mass concentration of 45% and 5 parts of mannose.
The chitosan mixture consists of chitosan with deacetylation degree of 80%, chitosan quaternary ammonium salt and hydroxypropyl chitosan according to the mass ratio of 5: 3: 1.
The preparation method comprises the following steps:
s1: taking a chitosan mixture, a glucose aqueous solution and mannose according to the specified mass part, placing the mixture into a reactor, adding an acetic acid aqueous solution with the volume concentration of 1.5%, wherein the adding amount of the acetic acid aqueous solution is 6 times of the total mass of the chitosan mixture, the glucose aqueous solution and the mannose, and stirring until the mixture is completely dissolved to prepare a mixed solution;
s2: heating the mixed solution prepared in the step S1 at the constant temperature of 55 ℃ for 15min to obtain a quick hemostatic agent;
s3: dividing the quick hemostatic medicament prepared in the step S2 into a quick hemostatic medicament A and a quick hemostatic medicament B, wherein the volume ratio of the quick hemostatic medicament A to the quick hemostatic medicament B is 7: 4;
s4: soaking the sponge cushion layer into the quick hemostatic agent A prepared in the step S3, taking out after 10h, and drying for 2h at 60 ℃ by adopting a drying oven to obtain a synergistic sponge cushion layer A;
s5: taking out the synergistic sponge cushion layer A prepared in the step S4, uniformly spraying the quick hemostatic agent B prepared in the step S3 on the synergistic sponge cushion layer A, and drying for 0.5h at 60 ℃ by adopting a drying oven to obtain a synergistic sponge cushion layer B;
s6: and (5) sticking the synergistic sponge cushion layer B prepared in the step (S5) to the middle section of the adhesive tape, and covering the surfaces of the adhesive tape and the sponge cushion layer with isolation paper to obtain the synergistic sponge cushion layer B.
Example 2A Chitosan sponge pad band-aid for rapid hemostasis
Quick hemostasis chitosan foam-rubber cushion woundplast comprises medical barrier paper, sponge bed course, viscose cloth, and the sponge bed course is located the viscose cloth middle section, and the barrier paper adheres to on viscose cloth and sponge bed course, contain quick hemostasis medicament in the sponge bed course, quick hemostasis medicament comprises following component and part by mass: 55 parts of chitosan mixture, 15 parts of glucose aqueous solution with the mass concentration of 55% and 8 parts of mannose.
The chitosan mixture consists of chitosan with deacetylation degree of 90%, chitosan quaternary ammonium salt and hydroxypropyl chitosan according to the mass ratio of 8: 2: 1.
The preparation method comprises the following steps:
s1: taking a chitosan mixture, a glucose aqueous solution and mannose according to the specified mass part, placing the mixture into a reactor, adding an acetic acid aqueous solution with the volume concentration of 2.5%, wherein the adding amount of the acetic acid aqueous solution is 20 times of the total mass of the chitosan mixture, the glucose aqueous solution and the mannose, and stirring until the mixture is completely dissolved to prepare a mixed solution;
s2: heating the mixed solution prepared in the step S1 at a constant temperature of 70 ℃ for 30min to obtain a quick hemostatic agent;
s3: dividing the quick hemostatic medicament prepared in the step S2 into a quick hemostatic medicament A and a quick hemostatic medicament B, wherein the volume ratio of the quick hemostatic medicament A to the quick hemostatic medicament B is 9: 2;
s4: soaking the sponge cushion layer into the quick hemostatic agent A prepared in the step S3, taking out after 25h, and drying for 4h at 70 ℃ by adopting a drying oven to obtain a synergistic sponge cushion layer A;
s5: taking out the synergistic sponge cushion layer A prepared in the step S4, uniformly spraying the quick hemostatic agent B prepared in the step S3 on the synergistic sponge cushion layer A, and drying for 2 hours at 70 ℃ by adopting a drying oven to obtain a synergistic sponge cushion layer B;
s6: and (5) sticking the synergistic sponge cushion layer B prepared in the step (S5) to the middle section of the adhesive tape, and covering the surfaces of the adhesive tape and the sponge cushion layer with isolation paper to obtain the synergistic sponge cushion layer B.
Example 3A Chitosan sponge pad band-aid for rapid hemostasis
Quick hemostasis chitosan foam-rubber cushion woundplast comprises medical barrier paper, sponge bed course, viscose cloth, and the sponge bed course is located the viscose cloth middle section, and barrier paper adheres to on viscose cloth and sponge bed course, contain quick hemostasis medicament in the sponge bed course, quick hemostasis medicament is by following component and part by mass: 48 parts of chitosan mixture, 12 parts of glucose aqueous solution with the mass concentration of 50% and 7 parts of mannose.
The chitosan mixture consists of chitosan with deacetylation degree of 85%, chitosan quaternary ammonium salt and hydroxypropyl chitosan according to the mass ratio of 7:2.5: 1.
The preparation method comprises the following steps:
s1: taking a chitosan mixture, a glucose aqueous solution and mannose according to the specified mass part, placing the mixture into a reactor, adding an acetic acid aqueous solution with the volume concentration of 2%, wherein the adding amount of the acetic acid aqueous solution is 14 times of the total mass of the chitosan mixture, the glucose aqueous solution and the mannose, and stirring until the acetic acid aqueous solution is completely dissolved to prepare a mixed solution;
s2: heating the mixed solution prepared in the step S1 at a constant temperature of 60 ℃ for 20min to obtain a quick hemostatic agent;
s3: dividing the quick hemostatic medicament prepared in the step S2 into a quick hemostatic medicament A and a quick hemostatic medicament B, wherein the volume ratio of the quick hemostatic medicament A to the quick hemostatic medicament B is 8: 3;
s4: soaking the sponge cushion layer into the quick hemostatic agent A prepared in the step S3, taking out after 16h, and drying for 3h at 65 ℃ by adopting a drying oven to obtain a synergistic sponge cushion layer A;
s5: taking out the synergistic sponge cushion layer A prepared in the step S4, uniformly spraying the quick hemostatic agent B prepared in the step S3 on the synergistic sponge cushion layer A, and drying for 1.5 hours at 65 ℃ by adopting a drying oven to obtain a synergistic sponge cushion layer B;
s6: and (5) sticking the synergistic sponge cushion layer B prepared in the step (S5) to the middle section of the adhesive tape, and covering the surfaces of the adhesive tape and the sponge cushion layer with isolation paper to obtain the synergistic sponge cushion layer B.
Comparative example 1 quick hemostasis chitosan sponge mat band-aid
Quick hemostasis chitosan foam-rubber cushion woundplast comprises medical barrier paper, sponge bed course, viscose cloth, and the sponge bed course is located the viscose cloth middle section, and the barrier paper adheres to on viscose cloth and sponge bed course, contain quick hemostasis medicament in the sponge bed course, quick hemostasis medicament comprises following component and part by mass: 48 parts of chitosan mixture, 12 parts of glucose aqueous solution with the mass concentration of 50% and 7 parts of mannose.
The chitosan mixture consists of chitosan with deacetylation degree of 85%, chitosan quaternary ammonium salt and hydroxypropyl chitosan according to the mass ratio of 1: 1.
The preparation method is similar to example 3.
The difference from example 3 is that the chitosan mixture consists of chitosan with deacetylation degree of 85%, chitosan quaternary ammonium salt and hydroxypropyl chitosan according to mass ratio of 1: 1.
Comparative example 2, a chitosan sponge pad band-aid for rapid hemostasis
Quick hemostasis chitosan foam-rubber cushion woundplast comprises medical barrier paper, sponge bed course, viscose cloth, and the sponge bed course is located the viscose cloth middle section, and the barrier paper adheres to on viscose cloth and sponge bed course, contain quick hemostasis medicament in the sponge bed course, quick hemostasis medicament comprises following component and part by mass: 48 parts of chitosan mixture, 12 parts of glucose aqueous solution with the mass concentration of 50% and 7 parts of mannose.
The chitosan mixture consists of chitosan with deacetylation degree of 85%, chitosan quaternary ammonium salt and hydroxypropyl chitosan according to the mass ratio of 7:2.5: 1.
The preparation method comprises the following steps:
s1: taking a chitosan mixture, a glucose aqueous solution and mannose according to the specified mass part, placing the mixture into a reactor, adding an acetic acid aqueous solution with the volume concentration of 2%, wherein the adding amount of the acetic acid aqueous solution is 14 times of the total mass of the chitosan mixture, the glucose aqueous solution and the mannose, and stirring until the acetic acid aqueous solution is completely dissolved to prepare a mixed solution;
s2: heating the mixed solution prepared in the step S1 at a constant temperature of 60 ℃ for 20min to obtain a quick hemostatic agent;
s3: soaking the sponge cushion layer into the quick hemostatic prepared in the step S2, taking out after 16h, and drying for 3h at 65 ℃ by adopting a drying oven to obtain a synergistic sponge cushion layer;
s4: and (5) adhering the synergistic sponge cushion layer prepared in the step (S3) to the middle section of the adhesive tape, and covering the surfaces of the adhesive tape and the sponge cushion layer with isolation paper to obtain the synergistic sponge cushion.
Comparative example 3, a chitosan sponge pad band-aid for rapid hemostasis
Quick hemostasis chitosan foam-rubber cushion woundplast comprises medical barrier paper, sponge bed course, viscose cloth, and the sponge bed course is located the viscose cloth middle section, and the barrier paper adheres to on viscose cloth and sponge bed course, contain quick hemostasis medicament in the sponge bed course, quick hemostasis medicament comprises following component and part by mass: 48 parts of chitosan mixture, 12 parts of glucose aqueous solution with the mass concentration of 50% and 7 parts of mannose.
The chitosan mixture consists of chitosan with deacetylation degree of 85%, chitosan quaternary ammonium salt and hydroxypropyl chitosan according to the mass ratio of 7:2.5: 1.
The preparation method comprises the following steps:
s1: taking a chitosan mixture, a glucose aqueous solution and mannose according to the specified mass part, placing the mixture into a reactor, adding an acetic acid aqueous solution with the volume concentration of 2%, wherein the adding amount of the acetic acid aqueous solution is 14 times of the total mass of the chitosan mixture, the glucose aqueous solution and the mannose, and stirring until the acetic acid aqueous solution is completely dissolved to prepare a mixed solution;
s2: heating the mixed solution prepared in the step S1 at a constant temperature of 60 ℃ for 20min to obtain a quick hemostatic agent;
s3: uniformly spraying the quick hemostatic agent prepared in the step S2 on the sponge cushion layer, and drying for 1h at 65 ℃ by adopting a drying oven to obtain a synergistic sponge cushion layer;
s4: and (5) adhering the synergistic sponge cushion layer prepared in the step (S3) to the middle section of the adhesive tape, and covering the surfaces of the adhesive tape and the sponge cushion layer with isolation paper to obtain the synergistic sponge cushion.
Test example I, test of hemostatic effect of chitosan sponge pad band-aid for quick hemostasis
1. Test materials: the chitosan sponge pad adhesive bandage for quick hemostasis prepared in examples 1-3 and comparative examples 1-3.
2. Test subjects: 60 New Zealand white rabbits weigh 2-2.5 kg, and are half female and half male.
3. The test method comprises the following steps:
new Zealand white rabbits were randomly divided into 6 groups, i.e., example 1 group, example 2 group, example 3 group, comparative example 1 group, comparative example 2 group, and comparative example 3 group, each of which was 10, each of which was half female and male, and were hemostatic using the chitosan sponge pads for rapid hemostasis prepared in examples 1, example 2, example 3, comparative example 1, comparative example 2, and comparative example 3, respectively.
After the white rabbits are anesthetized, the ears are unhaired, a wound surface with the size of 1cm multiplied by 1mm is made in the center of the outer side of the ears by a scalpel, wherein the artery and the vein are transversely cut, but the ears are not cut through, so that blood is prevented from flowing out of the ears to influence the observation index. Immediately applying hemostasis measures according to groups after the wound surface is filled with blood, recording hemostasis time and blood loss, and calculating average hemostasis time and average blood loss of 10 rabbits in each group. The hemostasis time recording method comprises the following steps: and (5) sticking the adhesive bandage on the bleeding wound surface, slightly opening the adhesive bandage from one side every 4s to observe the hemostasis condition until bleeding stops, and recording the hemostasis time. The bleeding amount calculation method comprises the following steps: the chitosan sponge pad adhesive bandage for quick hemostasis before use is weighed by an analytical balance, placed in a weighing bottle after hemostasis and weighed in a sealing way, and the amount of bleeding is calculated, wherein the amount of bleeding (g) is the weight after hemostasis (g) -the weight before hemostasis (g).
4. And (3) test results:
the results of the tests are shown in table 1:
TABLE 1 hemostatic effect test results of the chitosan sponge pad band-aid for rapid hemostasis in examples and comparative examples
As can be seen from Table 1, the test results of the average hemostasis time and the average blood loss of the quick hemostasis chitosan sponge pad band-aid of the invention are obviously superior to those of the quick hemostasis chitosan sponge pad band-aid prepared by a comparative example, especially the quick hemostasis chitosan sponge pad band-aid of the embodiment 3 has the best performance, the average hemostasis time reaches 8s, and the average blood loss is as low as 0.0054g, so that the quick hemostasis chitosan sponge pad band-aid of the invention can quickly and strongly stanch, is non-toxic and non-irritant, has high use safety, is simple and convenient to implement in preparation method and low in preparation cost, and can be widely applied to treatment of small wounds.
Test example two, anti-inflammatory test of chitosan sponge pad adhesive bandage for rapid hemostasis
1. Test materials: the chitosan sponge pad adhesive bandage for quick hemostasis prepared in example 1, example 2, example 3 and comparative example 1.
2. Test subjects: 50 Kunming mice are selected, half of the mice are male and female, and the weight of the mice is 30 g.
3. The test method comprises the following steps:
dividing 50 mice into 5 groups at random, wherein the groups are respectively a control group, an example 1 group, an example 2 group, an example 3 group and a comparative example 1 group, each group comprises 10 mice, the right ear of each group of mice is uniformly coated with 50 mu L of dimethylbenzene, the left ear is used as a control, and the right ear of the control group of mice is used with physiological saline 2 hours after inflammation; the quick hemostatic chitosan sponge pad adhesive bandages prepared in example 1, example 2, example 3 and comparative example 1 were applied to the right ears of the mice in the example 1 group, the example 2 group, the example 3 group and the comparative example 1 group.
And (3) taking off the vertebra after the medicine is applied for 12h, killing the patient, cutting two ears along the auricle, punching round ear pieces on the same parts of the left ear and the right ear by using an 8mm puncher, weighing, and calculating the ear weight difference, wherein the ear weight difference is equal to the weight of the round ear piece of the right ear and the weight of the round ear piece of the left ear.
4. And (3) test results:
the test results are shown in table 2.
TABLE 2 influence of chitosan sponge pad adhesive bandage for rapid hemostasis on mouse ear shell xylene-induced swelling
| Group of | Ear weight difference (mg) |
| Control group | 9.25 |
| EXAMPLE 1 group | 4.56 |
| EXAMPLE 2 group | 4.72 |
| EXAMPLE 3 group | 4.38 |
| Comparative example 1 group | 6.43 |
As can be seen from table 2, the groups of example 1, example 2 and example 3 can significantly reduce the difference in ear weight of the mice and have better anti-inflammatory effect compared with the control group, which indicates that the quick hemostatic chitosan sponge pad adhesive bandage prepared by the invention has significant anti-inflammatory effect, wherein the effect of example 3 is the best example of the invention; the effect of example 3 is superior to that of comparative example 1.
Test example III analgesic test of chitosan sponge pad bandage for quick hemostasis
1. Test materials: the chitosan sponge pad adhesive bandage for quick hemostasis prepared in example 1, example 2, example 3 and comparative example 1.
2. Test subjects: 60 Kunming mice are selected, half of the mice are female and half of the mice, and the weight of the mice is 30 g.
3. Establishing a mouse hot plate pain model:
60 mice are placed on a hot plate pain measuring instrument, the time of licking the feet is less than 5s or more than 30s, the cutoff time is set to be 60s for preventing the feet from being scalded, the latency period of the reaction of licking the feet by the mice is used as the pain threshold index, and the mice with the time of licking the feet being more than 5s and less than 30s are screened.
4. The test method comprises the following steps:
50 qualified mice are selected and randomly divided into 5 groups, and each group comprises 10 mice, namely a model group, an example 1 group, an example 2 group, an example 3 group and a comparative example 1 group. Saline was administered to the right ear of control mice; the quick hemostatic chitosan sponge pad adhesive bandages prepared in example 1, example 2, example 3 and comparative example 1 were applied to the right ears of the mice in the example 1 group, the example 2 group, the example 3 group and the comparative example 1 group.
And measuring the time of the foot licking reaction at 60min and 120min after the last drug.
5. And (3) test results:
the test results are shown in table 3.
TABLE 3 mouse licking latency time Table
| Group of | Licking time(s) after 60min | Licking time(s) after 120min |
| Model set | 19.8 | 22.9 |
| EXAMPLE 1 group | 33.4 | 35.6 |
| EXAMPLE 2 group | 31.6 | 33.2 |
| EXAMPLE 3 group | 35.7 | 37.8 |
| Comparative example 1 group | 23.8 | 25.9 |
As can be seen from table 3, compared with the model group, the rapid hemostatic chitosan sponge pad band-aid prepared according to the present invention can significantly prolong the mouse licking time, and the analgesic effect is better than that of the rapid hemostatic chitosan sponge pad band-aid prepared according to comparative example 1.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.
Claims (1)
1. The utility model provides a chitosan foam-rubber cushion woundplast fast stanchs, comprises medical barrier paper, sponge bed course, viscose cloth, and the sponge bed course is located the viscose cloth middle section, and the barrier paper adheres to on viscose cloth and sponge bed course, a serial communication port, contain quick hemostasis medicament in the sponge bed course, quick hemostasis medicament comprises following component and part by mass: 48 parts of chitosan mixture, 12 parts of glucose aqueous solution with the mass concentration of 50% and 7 parts of mannose;
the chitosan mixture consists of chitosan with deacetylation degree of 85%, chitosan quaternary ammonium salt and hydroxypropyl chitosan according to the mass ratio of 7:2.5: 1;
the preparation method of the chitosan sponge cushion band-aid for rapid hemostasis comprises the following steps:
s1: taking a chitosan mixture, a glucose aqueous solution and mannose according to the specified mass part, placing the mixture into a reactor, adding an acetic acid aqueous solution with the volume concentration of 2%, wherein the adding amount of the acetic acid aqueous solution is 14 times of the total mass of the chitosan mixture, the glucose aqueous solution and the mannose, and stirring until the acetic acid aqueous solution is completely dissolved to prepare a mixed solution;
s2: heating the mixed solution prepared in the step S1 at a constant temperature of 60 ℃ for 20min to obtain a quick hemostatic agent;
s3: dividing the quick hemostatic medicament prepared in the step S2 into a quick hemostatic medicament A and a quick hemostatic medicament B, wherein the volume ratio of the quick hemostatic medicament A to the quick hemostatic medicament B is 8: 3;
s4: soaking the sponge cushion layer into the quick hemostatic agent A prepared in the step S3, taking out after 16h, and drying for 3h at 65 ℃ by adopting a drying oven to obtain a synergistic sponge cushion layer A;
s5: taking out the synergistic sponge cushion layer A prepared in the step S4, uniformly spraying the quick hemostatic agent B prepared in the step S3 on the synergistic sponge cushion layer A, and drying for 1.5 hours at 65 ℃ by adopting a drying oven to obtain a synergistic sponge cushion layer B;
s6: and (5) sticking the synergistic sponge cushion layer B prepared in the step (S5) to the middle section of the adhesive tape, and covering the surfaces of the adhesive tape and the sponge cushion layer with isolation paper to obtain the synergistic sponge cushion layer B.
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| CN109125790A (en) * | 2018-08-25 | 2019-01-04 | 山东康力医疗器械科技有限公司 | A kind of styptic sponge structure made of chitosan |
| CN109289078B (en) * | 2018-11-02 | 2020-06-19 | 广东药科大学 | Long-acting antibacterial band-aid |
| CN114452081A (en) * | 2022-02-22 | 2022-05-10 | 河南省洛阳正骨医院（河南省骨科医院） | Rapid hemostasis application |
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| CN104324409A (en) * | 2014-09-26 | 2015-02-04 | 张明 | Novel quickly bleed-stooping band-aid |
| CN104307027A (en) * | 2014-09-28 | 2015-01-28 | 张亚峰 | A medical chitosan composite dressing capable of rapidly arresting bleeding |
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