TW201333187A - Multi-functional photo-bioreactor system for microalgae and sampling method thereof - Google Patents

Abstract

A multi-functional photo-bioreactor system for microalgae and a sampling method thereof are provided. The multi-functional photo-bioreactor system includes a cultivation system, an air supplying system and an environment monitoring system. The cultivation system has at least one tank for cultivating at least one type of microalgal medium. The amount of the tanks can be changed according to actual needs, while growth conditions of each of the tanks, such as nutrient, carbon source and light shielding control, can be individually adjusted. Thus, various different growth conditions can be provided to cultivate the same type or different type of microalgae, so as to meet the needs of intermediate scale pretest requirements before the mass production of microalgae. Furthermore, when a user wants to sample microalgae in one of the tanks for measurement, air exhaustion pipe of the to-be-sampled tank and air valves of the remaining tanks can be closed, so that extra pressure can be provided to a sampling pipe of the to-be-sampled tank for triggering a siphon phenomenon to induce the liquid of medium flowing out of the tank, so as to rapidly sample the microalgae fluid from the desired tanks.

Description

Multifunctional microalgae photobioreactor and sampling method thereof

The invention relates to a multifunctional microalgae photobioreactor and a sampling method thereof, in particular to a multifunctional microalgae suitable for outdoor breeding microalgae, modular disassembly and assembly culture barrel, and convenient for collecting algae liquid samples. Photobioreactor and its sampling method.

Since micro-algae can fix carbon dioxide by photosynthetic cooperation and convert it into organic biomass, these biomass can be used in various technical fields and reprocessing, such as health. Food, food additives, biopharmaceuticals, green energy, aquaculture, wastewater treatment, etc. The advantage of using microalgae is that microalgae can continuously convert carbon dioxide through light energy to convert and store it into biomass, so it can meet the green environmental protection requirements of sustainable use. Taking microalgae production of oil and fat as an example, under the same culture area, the microalgae oil production capacity is dozens of times more than that of traditional oil plant crops. Furthermore, the growth of microalgae generally requires only carbon dioxide and solar energy from the air, and the growth is rapid, and the number of annual harvests is large. Therefore, the microalgae production mode has the advantages of renewability, cost reduction, and industrial pollution reduction.

In order to meet the demand for mass production of microalgae, relevant researchers have developed various photo-bioreactors for the production of various microalgae, which can be divided into indoor breeding systems and outdoor farming systems. The indoor breeding system usually uses an artificial light source, such as an incandescent lamp or a light source of a light-emitting diode (LED), while the outdoor farming system directly uses sunlight as a light source, and the breeding system can be roughly divided into a closed photobioreactor. (closed photobioreactor, PBR) and the open streamway pond.

Furthermore, the greenhouse effect caused by the increase in the concentration of carbon dioxide (CO ₂ ) in the air has become a major environmental problem. The CO ₂ emissions of Taiwan's petrochemical industry and power plants are high, and how to effectively reduce CO ₂ by fixing CO ₂ . The CO ₂ concentration is a key issue. In addition to physical adsorption, chemical absorption, and membrane separation processes, bio-fixation of CO ₂ is one of the viable options for reducing CO ₂ concentration. Microalgae can absorb a large amount of CO ₂ during the process of photosynthesis and its growth is fast, about ten times higher than the fixed CO ₂ efficiency of terrestrial plants, so it is considered to be a highly efficient bio-fixed CO ₂ species. The farmer used in the outdoor microalgae adsorption carbon benefit experiment is often referred to as an outdoor photobioreactor. In the cultivation process of microalgae, in addition to the monitoring of environmental conditions, the monitoring of algae water quality and algae growth indicators (such as optical density, OD) is the focus of observation on whether algae can be successfully cultured outdoors. Therefore, how to design appropriate equipment to provide a variety of different growth conditions to breed and experiment to find the most suitable growth conditions for microalgae, or to find the optimal growing microalgae species under the same growth conditions, and provide fast and convenient Sampling methods for efficient recovery of algae and extensive analysis are key technologies for future large-scale farming systems and industrial applications such as carbon sequestration.

However, the culture tanks or tubes of the current indoor culture systems and outdoor culture systems are mostly interconnected and can only be regulated into a single growth condition, and there is no modular design to provide multiple independent but simultaneously fast A culture tank or tube that adjusts the growth conditions. At the same time, most of the existing culture tanks or tubes are small indoor culture equipment or large outdoor culture equipment, lacking a medium-sized equipment to supply pre-test operations for microalgae production or intermediate-scale diversified mass production. operation.

Therefore, it is necessary to provide a multifunctional microalgae photobioreactor and its sampling method to solve the problems existing in the conventional technology.

The main object of the present invention is to provide a multifunctional microalgae photobioreactor and a sampling method thereof, which have a modular design and are suitable for outdoor breeding microalgae, and can quickly perform modular disassembly and culture barrel according to the needs of use. The growth conditions of each culture barrel, such as nutrition, carbon source and shading control, can be set independently. Therefore, a variety of different growth conditions can be provided to breed the same kind of microalgae (or a variety of different microalgae), so as to find out the microalgae. The optimal growth conditions (or which microalgae grows optimally under certain growth conditions) can indeed meet the demand for pre-test or multi-media production of microalgae. Obtain various monitoring data for future planning and design of large-scale (ton-class) aquaculture systems and industrial applications such as carbon sequestration; in addition, it can also be applied to medium-sized (hundreds of liters) small-scale microalgae independent production operations. Therefore, the functionality of the microalgae photobioreactor can be greatly increased and meet various application requirements.

A secondary object of the present invention is to provide a multifunctional microalgae photobioreactor and a sampling method thereof, wherein each culture tank is a modular unit, and if the algae liquid of a certain culture tank is to be tested, Closing the exhaust pipe of the culture drum and the air valve of other culture drums, and providing additional pressurized air to the sampling pipe in the sampling culture tank to generate siphon phenomenon, thereby being able to take turns to the outdoor experimental culture barrel The rapid sampling of the algae liquid provides a quick and easy sampling method for efficient recovery of algae or routine and frequent sampling of algae without the need for additional sampling-specific suction motor components. Therefore, it is indeed advantageous to improve the convenience of the sampling operation.

In order to achieve the above object, the present invention provides a multifunctional microalgae photobioreactor comprising: a culture system having at least one culture tank for accommodating and cultivating at least one algae liquid, and each of the culture barrels is provided with a a gas pipe, an exhaust pipe and a sampling pipe, wherein the bottom end of the gas supply pipe and the sampling pipe are immersed in the algae liquid; and a gas supply system having an air supply end, a carbon source supply end and at least one gas mixing tank, The air supply end supplies air to each of the gas mixing tanks via a first gas valve, and the carbon source supply end supplies carbon dioxide to each of the gas mixing tanks via a second gas valve, each of the gas mixing tanks and the air The carbon dioxide is uniformly mixed and supplied to the algae liquid in each of the culture tanks through the respective air supply pipes; and an environmental monitoring system having at least one water temperature sensor, a temperature sensor and a light sensor, the water The temperature sensors are respectively disposed in each of the culture barrels, and the temperature sensor and the illumination sensor are disposed outside the culture barrel.

In an embodiment of the present invention, a shading system is further disposed above the culture tub, the shading system has at least one shading net and at least one scrolling device for shielding part of the sunlight, the scrolling The device is configured to open or retract the shade net, thereby adjusting the sunshine intensity of the culture barrel to improve the control convenience of the sunshine intensity.

In an embodiment of the present invention, a sprinkler system is further disposed above the culture tub, the sprinkler system having at least one sprinkler head to sprinkle water to cool the culture tub.

In an embodiment of the present invention, the culture barrel is a hollow light-transmissive barrel made of a light-transmitting material such as plastic or glass having a light transmittance of 80-99%. For example, the light-transmitting material is preferably acrylic. Or polycarbonate (PC), which provides about 85% light transmission.

In an embodiment of the invention, the culture tank is selected from a distilled water bucket of polycarbonate (PC) material.

In an embodiment of the present invention, the inner surface of the transparent barrel of the culture barrel is coated with an anion coating to prevent the microalgae or other microorganisms from adhering to the surface to form a biofilm to affect the light transmittance, thereby being advantageous. In order to maintain the light transmission of the culture barrel, and relatively reduce the need for maintenance and cleaning.

In an embodiment of the present invention, the air supply system further has an air filter, the air filter is located between the air supply end and the first air valve to filter out impurities contained in the air flowing through And unnecessary extra particles such as dust.

In an embodiment of the invention, the air supply system further has a total air valve and a total flow meter disposed between the air filter and the first air valve.

In an embodiment of the invention, the gas supply system further has a first flow meter disposed between the first gas valve and the gas mixing tank to monitor the air flow rate.

In an embodiment of the invention, the gas supply system further has a second flow meter disposed between the second gas valve and the gas mixing tank to monitor the carbon dioxide flow rate.

In an embodiment of the invention, the bottom end of the air supply pipe is further provided with a bubble stone.

In order to achieve the above object, the present invention provides another multifunctional microalgae photobioreactor comprising: a culture system having at least one culture tank for accommodating and cultivating at least one algae liquid, and each of the culture barrels is provided with a a gas supply pipe, an exhaust pipe and a sampling pipe, the bottom end of the gas supply pipe and the sampling pipe being immersed in the algae liquid; and a gas supply system having an air supply end, a carbon source supply end and at least one gas mixing tank The air supply end supplies air to each of the gas mixing tanks via a first gas valve, and the carbon source supply end supplies carbon dioxide to each of the gas mixing tanks via a second gas valve, each of the gas mixing tanks The air and carbon dioxide are uniformly mixed and supplied to the algae liquid in each of the culture tanks through the respective air supply pipes.

In order to achieve the above object, the present invention further provides a sampling method for a multifunctional microalgae photobioreactor, comprising the steps of: providing a multifunctional microalgae photobioreactor as described above, having a plurality of the culture barrels; Controlling that the first gas valve of one of the culture barrels to be sampled is in an open state and the exhaust pipe is in a closed state, and the first and second gas valves of the remaining culture drums are closed; and by maintaining the air supply The air supply to the end of the algae liquid in the culture tank to be sampled causes the siphon phenomenon to accumulate due to the air input, so that the algae liquid in the culture tank is automatically output to the output end of the algae liquid through the sampling tube. It's extremely easy to sample without opening the cap.

The above and other objects, features and advantages of the present invention will become more <RTIgt; Furthermore, the directional terms mentioned in the present invention, such as "upper", "lower", "before", "after", "left", "right", "inside", "outside" or "side", etc. Just refer to the direction of the additional schema. Therefore, the directional terminology used is for the purpose of illustration and understanding of the invention.

Referring to FIG. 1 , the multifunctional microalgae photobioreactor of the first embodiment of the present invention mainly comprises: a culture system 10, a gas supply system 20, an environmental monitoring system 30, a shading system 40, and a sprinkler system. Water system 50. The present invention will be described in detail below with reference to Figs. 1 to 4 in detail of the detailed construction, assembly relationship, and operation principle of the above-described respective elements of the first embodiment.

Referring to Figures 1 and 2, the culture system 10 of the first embodiment of the present invention is mainly used for cultivating various culture solutions of microalgae (abbreviated as algae liquid). The culture system 10 has at least one culture tank 11 for accommodating and cultivating at least one algae liquid, and each of the culture barrels 11 is provided with a stopper 12, an air supply tube 13, an exhaust pipe 14, and a sampling tube 15. The culture barrel 11 is a hollow light-transmissive barrel made of a light-transmitting material such as plastic or glass, and the light-transmitting material is preferably acrylic or polycarbonate (PC), which can provide a light transmittance of about 85%. . In an embodiment of the present invention, the culture tub 11 may be selected from a distilled water bucket of PC material, and the source may be a new finished product or a recycled old product. The capacity of the distilled water bucket is about 18 liters, and its capacity just meets the capacity required for the pre-test of the microalgae mass production. The air supply pipe 13, the exhaust pipe 14 and the sampling pipe 15 are all disposed on the cork 12, and may be selected from a glass tube, a plastic tube, a rubber tube or a combination thereof. The bottom end of the air supply pipe 13 and the bottom end of the sampling pipe 15 are immersed in the algae liquid, and the bottom end of the exhaust pipe 14 is located above the liquid surface of the algae liquid. The bottom end of the air supply pipe 13 is close to the inner bottom surface of the culture tank 11, and the bottom end of the air supply pipe 13 is further provided with a bubble stone 16 for pumping the algae liquid. The bottom end of the sampling tube 15 is located in the algae liquid of the culture tank 11.

If necessary, the inner surface of the transparent barrel of the culture barrel 11 may alternatively be coated with an anion coating (not shown) to prevent the microalgae or other microorganisms from adhering to the surface to form a biofilm to affect the light transmittance. Therefore, it is advantageous to maintain the light transmittance of the culture barrel and relatively reduce the need for maintenance and cleaning.

Referring again to Figures 1 and 2, the gas supply system 20 of the first embodiment of the present invention is mainly used to supply air and carbon dioxide. The gas supply system 20 mainly has an air supply end 21, a carbon source supply end 22, and a gas mixing tank 23. The air supply end 21 is, for example, an air pump that can suck in and pressurize the air of the external atmosphere. The air supply end 21 sequentially passes through an air filter 24, a total air valve 251, and a total flow meter. 252, a first air valve 261 and a first flow meter 262 supply pressurized air to the gas mixing tank 23, wherein the air filter 24 is located between the air supply end 21 and the total air valve 251, and has At least one filter screen filters the pressurized air passing through, and removes various organic and inorganic impurities and dust and other unnecessary particles in the pressurized air.

Moreover, the total air valve 251 and the first air valve 261 are, for example, a ball valve, and the total air valve 251 is located between the air filter 24 and the first air valve 261, and can be used for manual operation. Or, the total flow rate of the pressurized air of all the culture tanks 11 is automatically adjusted, and the first gas valve 261 is used for manually or automatically adjusting the flow rate of the respective pressurized air of each of the culture tanks 11. The total flow meter 252 is located between the total air valve 251 and the first air valve 261, and is used for monitoring and recording the total flow rate of the pressurized air of all the culture tanks 11, and the first flow meter 262 is located at the first gas. The valve 261 and the gas mixing tank 23 are respectively used for monitoring and recording the flow rate values of the respective pressurized air of each of the culture tanks 11. Basically, the present embodiment only adjusts and monitors the total pressurized air flowing to all the culture tanks 11 with a single group of total air valve 251 and total flow meter 252, and sets a set of first air valves for each of the culture barrels 11 respectively. 261 and the first flow meter 262 each adjust and monitor the individual pressurized air flowing to each of the culture drums 11.

In addition, the carbon source supply end 22 is, for example, at least one can of carbon dioxide gas cylinder or indirectly from the factory flue gas, which can supply pressurized carbon dioxide to the second gas valve 271 and a second flow meter 272 in sequence. The gas mixing tank 23, wherein the second gas valve 271 is, for example, a ball valve, is located between the carbon source supply end 22 and the second flow meter 272, and can be used to manually or automatically adjust the flow rate of the pressurized carbon dioxide. The second flow meter 272 is disposed between the second gas valve 271 and the gas mixing tank 23 and can be used to monitor and record the flow rate of carbon dioxide. In order to provide different growth conditions, the present embodiment provides a set of second gas valve 271 and second flow meter 272 for each culture tank 11 to provide different (or the same) carbon dioxide supply amount to each culture tank 11.

In addition, the gas mixing tank 23 is a hollow tubular body, and the top end thereof is connected to the second flow meter 272, the second gas valve 271 and the carbon source supply end 22, and the other through the two tubes. The tubular body is sequentially connected to the first flow meter 262, the first air valve 261, the total flow meter 252, the total air valve 251, the air filter 24, and the air supply end 21. The bottom end of the gas mixing tank 23 is connected to the culture tank 11 via the air supply pipe 13. The gas mixing tank 23 is for uniformly mixing air and carbon dioxide, and supplies the mixed gas to the algae liquid in the culture tank 11 for photosynthesis.

Referring to FIG. 1 and FIG. 2 again, the environmental monitoring system 30 of the first embodiment of the present invention is mainly used to monitor the growth condition parameters such as the ambient temperature and the sunshine intensity inside and outside the culture barrel 11. The environmental monitoring system 30 mainly has a water temperature sensor 31, an air temperature sensor 32 and an illumination sensor 33. The water temperature sensor 31 is disposed in the culture tank 11 and at least partially immersed in the algae liquid for monitoring and recording the water temperature change value of the algae liquid. The temperature sensor 32 and the illumination sensor 33 are disposed outside the culture barrel 11 for monitoring and recording the temperature change value of the surrounding environment and the change value of the sunshine intensity, and the change value of the sunshine intensity is the photosynthesis effective light ( Reference value of photosynthetic active radiation, PAR). The water temperature sensor 31 and the temperature sensor 32 are, for example, a composite type sensor of the type HOBO UA-002-64 with temperature and sunshine intensity, which can also assist in measuring the intensity of sunlight. The illumination sensor 33 is, for example, a light sensor product of the type Li-Cor LI-193SA.

Referring to FIGS. 1 and 2 again, the shading system 40 of the first embodiment of the present invention is mainly used to adjust the sunshine intensity of the culture tub 11 to improve the control convenience of the sunshine intensity. The shading system 40 is disposed substantially above the culture tub 11 and has at least one shading net 41 and at least one scrolling device 42. For example, two shading nets 41 and two scrolling devices 42 are provided, but the number can be practical. The demand is increased or decreased, so it is not limited to this. The shade net 41 is used to shield part of the sunlight, and the scrolling device 42 is used to open or retract the shade net 41. In the present embodiment, the shade net 41 can be selected from various semi-translucent web materials, such as a mesh material woven from black nylon rope, but is not limited thereto. When two sets of shade nets 42 are provided, they may have a light transmittance (or other different % transmittance) of 80% and 60%, respectively, for being opened separately or simultaneously to shield part of the sunlight and avoid supplying excessive light intensity. The algae liquid in the culture tank 11. Furthermore, the scrolling device 42 is used to open or retract the shade net 42. The greater the degree of shielding of the shade net 42 is, the more obvious the degree of absorption of the sunlight by the culture drum 11 is, so that the appropriate sunlight shielding rate can be provided to regulate the light intensity of the sunlight on the culture drum 11 (and Sun temperature). The shade net 42 can be shaded or not shaded simultaneously for all the culture tanks 11; or it can be designed such that each of the culture tanks 11 has a set of shade nets 42 so that each of the culture tanks 11 can independently adjust the light intensity.

Referring to Figures 1 and 2 again, the sprinkler system 50 of the first embodiment of the present invention is mainly used for watering and cooling the culture tub 11. The sprinkler system 50 is preferably disposed above the culture tub 11 but below the shade net 42. The sprinkler system 50 has at least one sprinkler head 51 for sprinkling the culture tub 11 to reduce the environment. Temperature and control water temperature fall within the set value. The sprinkler head 51 can simultaneously spray water to cool all the culture tanks 11; or it can be designed such that each of the culture tanks 11 has a sprinkler head 51 for sprinkling or not sprinkling water, respectively.

Referring to FIGS. 1 and 2, when the multifunctional microalgae photobioreactor of the first embodiment of the present invention is installed and used in an outdoor environment, first, the same (or different) type is injected into each of the culture tanks 11. The culture solution of the microalgae is then covered with the stopper 12, wherein the air supply end 21 and the carbon source supply end 22 respectively supply pressurized air and carbon dioxide to the gas mixing tank 23, and then through the air supply tube 13 and the bubble stone 16 pump up to the algae solution to facilitate photosynthesis of the algae solution. At this time, according to the experimental or product requirements, the total air valve 251 and the first air valve 261 can be used to manually or automatically adjust the flow rate of the pressurized air of all or each of the culture tanks 11 and utilize the second air valve 271. The flow rate of carbon dioxide pressurized in each of the culture tanks 11 is manually or automatically adjusted to provide different gas mixing ratios, and different (or the same) carbon sources are provided for the same (or different) microalgae in each of the culture tanks 11. condition. Furthermore, if each of the culture tanks 11 has a set of shade nets 42, each of the culture tanks 11 can independently adjust the light intensity and the sun temperature; if each of the culture tanks 11 has a set of sprinkler heads 51, each of the culture tanks 11 can Independently sprinkle water to cool down. In this way, each culture tank 11 can be set to have a plurality of different growth condition parameters.

During the photosynthesis of the algae liquid, the water temperature sensor 31 is used for monitoring and recording the water temperature change value of the algae liquid, and the temperature sensor 32 is used for monitoring and recording the temperature change value of the surrounding environment, and the sense of illumination. The detector 33 is used to monitor and record the variation of the sunshine intensity (ie, the photosynthesis effective light reference value) of the surrounding environment.

In addition, as shown in Fig. 3, in order to further obtain data such as the optical density (OD) and the total amount of organic biomass (biomass) of the algae liquid, it is necessary to first sample the algae liquid for each of the culture tanks 11. A first embodiment of the present invention provides a sampling method for a multifunctional microalgae photobioreactor, comprising the following steps: Step (S01), providing a multifunctional microalgae photobioreactor as described above, having a plurality of The culture tank 11 is, for example, four; in step (S02), the first gas valve 261 of the culture drum 11 to be sampled is controlled to be in an open state and the exhaust pipe 14 is in a closed state, and the remaining culture drums 11 are controlled. The first and second gas valves 261, 271 are in a closed state, wherein the culture barrel 11 to be sampled, for example, refers to the third culture barrel 11 sorted from left to right in Fig. 3, while the exhaust pipe 14 is blocked by a plug 28. a state of being closed; and step (S03), by maintaining the air supply end 21 to continue to supply pressurized air, causing a siphon phenomenon due to accumulation of air pressure due to air input in the algae liquid in the culture drum 11 to be sampled The algae liquid in the culture tank 11 The output of the sampling tube 15 through the output terminal 17 a liquid alginate, wherein the alginate solution may refer to an output terminal 17 via a hose into a collection container output.

By the above sampling method, the present invention can quickly collect the algae liquid in the culture barrel 11 and thereby rapidly sample the algae liquid in the other culture barrels 11 of the outdoor experiment, thereby providing a quick and convenient sampling method, so that Efficient recovery of algae fluid or routine analysis of a large number of algae fluids without the need for additional sampling-specific suction motor components, thus facilitating the convenience of sampling operations.

Referring to FIG. 4, the multifunctional microalgae photobioreactor according to the second embodiment of the present invention is similar to the first embodiment of the present invention, and generally uses the same component name and figure number, but the difference between the two is characterized. Therefore, the multi-functional microalgae photobioreactor of the second embodiment can increase or decrease the number of the culture drums 11 according to the experiment or product demand, for example, the number of the culture barrels 11 is increased to six, which can be used separately. Different types of microalgae are cultured under the same growth conditions to culture the same microalgae under different growth conditions, or to culture the same kind of microalgae under the same growth conditions for batch experiment or medium-scale mass production. . In other embodiments, the number of the culture drums 11 may also be one, two or other numbers.

As described above, the inventions of Figures 1 to 4 have a modular design and are suitable for outdoor breeding, compared to the shortcomings of existing indoor farming systems and outdoor farming systems, such as lack of modular design and inability to provide diverse growth conditions. Microalgae, and can be quickly assembled and disassembled according to the needs of use. The growth conditions of nutrition, carbon source and shading control of each culture tank 11 can be independently set, so that various growth conditions can be provided to breed the same species. Microalgae (or a variety of different microalgae), in order to find out the optimal growth conditions of the microalgae (or which microalgae grows optimally under certain growth conditions), so it can meet the prenatal selection of microalgae Weak pre-test or multi-media production needs, and facilitate the acquisition of various monitoring data for future planning and design of large-scale (ton-class) aquaculture systems and industrial applications such as carbon sequestration; It can be applied to medium-sized (hundreds of liters) small-scale microalgae independent production operations, so it can greatly increase the functionality of microalgae photobioreactors and meet the needs of various applications.

Furthermore, each of the culture drums 11 of the present invention is a modular unit. If the algae liquid of a certain culture tank 11 is to be detected, the exhaust pipe 14 and other culture tanks 11 of the culture tank 11 can be closed. The first and second gas valves 261, 271, while providing additional pressurized air to the sampling tube 17 in the sample culture tank 11 to generate a siphon phenomenon, can be rotated in this way to the culture barrel 11 of the outdoor experiment. Rapid sampling of algae fluids provides fast and easy sampling for efficient recovery of algae or routine and large-scale sampling of algae without the need for additional sampling-specific suction motor components. It really helps to improve the convenience of sampling operations.

The present invention has been disclosed in its preferred embodiments, and is not intended to limit the invention, and the present invention may be modified and modified without departing from the spirit and scope of the invention. The scope of protection is subject to the definition of the scope of the patent application.

10. . . Training system

11. . . Culture barrel

12. . . Cork

13. . . Airway

14. . . exhaust pipe

15. . . Sampling tube

16. . . Bubble stone

17. . . Algae output

20. . . Gas supply system

twenty one. . . Air supply

twenty two. . . Carbon source

twenty three. . . Gas mixing tank

twenty four. . . Air filter

251. . . Total air valve

252. . . Total flow meter

261. . . First air valve

262. . . First flow meter

271. . . Second valve

272. . . Second flow meter

28. . . embolism

30. . . Environmental monitoring system

31. . . Water temperature sensor

32. . . Temperature sensor

33. . . Light sensor

40. . . Shading system

41. . . Shading net

42. . . Scrolling device

50. . . Sprinkler system

51. . . Sprinkler head

Fig. 1 is a schematic view showing the multifunctional microalgae photobioreactor of the first embodiment of the present invention.

Fig. 2 is a schematic view showing the use of the multifunctional microalgae photobioreactor of the first embodiment of the present invention.

Fig. 3 is a schematic view showing the use of the multifunctional microalgae photobioreactor of the first embodiment of the present invention when sampling.

Fig. 4 is a schematic view showing the multifunctional microalgae photobioreactor of the second embodiment of the present invention.

10. . . Training system

11. . . Culture barrel

12. . . Cork

13. . . Airway

14. . . exhaust pipe

15. . . Sampling tube

16. . . Bubble stone

17. . . Algae output

20. . . Gas supply system

twenty one. . . Air supply

twenty two. . . Carbon source

twenty three. . . Gas mixing tank

twenty four. . . Air filter

251. . . Total air valve

252. . . Total flow meter

261. . . First air valve

262. . . First flow meter

271. . . Second valve

272. . . Second flow meter

28. . . embolism

30. . . Environmental monitoring system

31. . . Water temperature sensor

32. . . Temperature sensor

33. . . Light sensor

40. . . Shading system

41. . . Shading net

42. . . Scrolling device

50. . . Sprinkler system

51. . . Sprinkler head

Claims (10)

A multifunctional microalgae photobioreactor comprising: a culture system having at least one culture tank for accommodating and cultivating at least one algae liquid, and each of the culture barrels is provided with a gas supply pipe, an exhaust pipe and a sampling a bottom end of the gas supply pipe and the sampling pipe is immersed in the algae liquid; a gas supply system having an air supply end, a carbon source supply end and at least one gas mixing tank, the air supply end respectively passing through a first gas The valve supplies air to each of the gas mixing tanks, and the carbon source supply end supplies carbon dioxide to each of the gas mixing tanks via a second gas valve, and each of the gas mixing tanks uniformly mixes the air and the carbon dioxide, respectively The air pipe is supplied to the algae liquid in each of the culture tanks; and an environmental monitoring system has at least one water temperature sensor, a temperature sensor and a light sensor, and the water temperature sensors are respectively disposed at the respective The culture barrel and the temperature sensor and the illumination sensor are disposed outside the culture barrel. The multifunctional microalgae photobioreactor according to claim 1, further comprising a shading system disposed above the culture tub, the shading system having at least one shading net and at least one scrolling device, The sunshade net is used to shield part of the sunlight, and the scrolling device is used to open or retract the sunshade net. The multi-functional microalgae photobioreactor according to claim 1, further comprising a sprinkler system disposed above the culture tub, the sprinkler system having at least one sprinkler head to the culture tub Sprinkle water to cool down. The multifunctional microalgae photobioreactor according to claim 1, wherein the culture barrel is a hollow light-transmissive barrel made of a light-transmitting material having a light transmittance of 80-99%. The multifunctional microalgae photobioreactor according to claim 4, wherein the light transmissive material is acrylic or polycarbonate. The multifunctional microalgae photobioreactor of claim 4, wherein the culture barrel is selected from the group consisting of distilled water buckets. The multifunctional microalgae photobioreactor according to claim 4, wherein the inner surface of the transparent barrel of the culture barrel is coated with an anion coating. The multifunctional microalgae photobioreactor according to claim 1, wherein the gas supply system further has a first flow meter and a second flow meter, wherein the first flow meter is disposed on the first gas valve And a gas mixing tank to monitor the air flow; and the second flow meter is disposed between the second gas valve and the gas mixing tank to monitor the carbon dioxide flow. A multifunctional microalgae photobioreactor comprising: a culture system having at least one culture tank for accommodating and cultivating at least one algae liquid, and each of the culture barrels is provided with a gas supply pipe, an exhaust pipe and a sampling a bottom end of the gas supply pipe and the sampling pipe is immersed in the algae liquid; and a gas supply system having an air supply end, a carbon source supply end and at least one gas mixing tank, the air supply end respectively passing through a first a gas valve supplies air to each of the gas mixing tanks, and the carbon source supply end supplies carbon dioxide to each of the gas mixing tanks via a second gas valve, wherein the gas mixing tank uniformly mixes the air and the carbon dioxide, respectively The air tube is supplied to the algae liquid in each of the culture tubs. A sampling method for a multifunctional microalgae photobioreactor, comprising the steps of: providing a multi-functional microalgae photobioreactor according to claim 1 or 9 which has a plurality of the culture barrels; The first and second gas valves of the culture barrel to be sampled are in an open state and the exhaust pipe is closed, and the first and second gas valves of the remaining culture drums are closed; and by maintaining the air The supply end continues to supply air, so that the air pressure in the culture tank in the culture tank to be sampled is caused by the air pressure to generate a siphon phenomenon, and the algae liquid in the culture tank is automatically output to the output of the algae liquid through the sampling tube. .