TW201328732A - Hydrophilic antimicrobial film - Google Patents

Abstract

The present invention provides a hydrophilic antimicrobial film obtained by blending a NSP-silver composite and a polymer material, wherein the NSP-silver composite is obtained by subjecting an organic dispersing agent, nano silicate plates (NSP) and a solution containing silver ions to a reduction reaction. The invention also provides a hydrophilic antimicrobial dressing material, comprising a hydrophilic antimicrobial film as described above and at least one releasing layer. The invention also provides a hydrophilic antimicrobial dressing material, comprising a hydrophilic antimicrobial film as described above and a waterproof backing layer, wherein the waterproof backing layer is to cover one surface of the hydrophilic antimicrobial film. The hydrophilic antimicrobial film of this invention possesses both the antibacterial property and water absorbability, and enables the nano silver particles to be effectively dispersed in the hydrophilic antimicrobial film.

Description

Hydrophilic antibacterial film

The invention relates to a hydrophilic antibacterial film, in particular to a hydrophilic antibacterial film obtained by kneading a nano-powder-silver composite with a polymer material and a hydrophilic antibacterial material prepared therefrom.

Generally, the application materials for wounds should have two main functions: one is antibacterial effect, that is, by blocking bacterial invasion and having bactericidal function to prevent wound infection; the other is water absorption performance, that is, by absorbing wound exudate and having Moisturizing function to improve wound healing and reduce pain during dressing change.

In recent years, silver-containing dressings with good antibacterial efficacy have become mainstream, such as ConvaTec's Aquacel-Ag and Smith & Nephew's Acticoat, among which dressings containing silver nanoparticles are most common. The antibacterial principle of silver is to quickly combine with the sulfhydryl groups in the bacteria after destroying the bacterial cell membrane, thereby producing an antibacterial effect. However, by adding silver outside, the silver is easily peeled off from the dressing, resulting in a decrease or loss of the antibacterial efficacy of the dressing. In addition, due to the strong van der Waals force between the nano-sized silver particles, it is easy to cause aggregation and is difficult to apply; generally, a dispersant is added during the preparation of the silver nanoparticles (for example, lemon). Trisodium citrate, chitosan, glucose or polyvinyl pyrrolidone, etc., thereby stably dispersing the silver nanoparticles. At present, commercially available silver-containing dressings generally have disadvantages such as poor water absorption and high cost. Therefore, there is still a demand for developing a hydrophilic antibacterial material having both antibacterial and water absorption properties in the industry.

Therefore, the first object of the present invention is to provide a hydrophilic antibacterial film which is obtained by kneading a nanosheet-silver composite with a polymer material, wherein the nanosheet-silver composite is composed of An organic dispersant, a nano-powder tablet and a solution containing silver ions are obtained by a reduction reaction.

A second object of the present invention is to provide a hydrophilic antimicrobial sheath comprising a hydrophilic antimicrobial film as described above and at least one release layer, wherein the release layer is detachably covering one surface of the hydrophilic antimicrobial film.

A third object of the present invention is to provide a hydrophilic antimicrobial sheath comprising a hydrophilic antimicrobial film as described above and a waterproof backing layer, wherein the waterproof backing layer covers one surface of the hydrophilic antimicrobial film.

The hydrophilic antibacterial film of the present invention has both antibacterial and water absorbing properties, and enables the silver nanoparticles to be effectively dispersed in the hydrophilic antibacterial film, and only a small amount of silver is used in the preparation process.

The hydrophilic antibacterial film of the present invention is obtained by kneading a nano-powder-silver composite with a polymer material, wherein the nano-powder-silver composite is composed of an organic dispersant, a nano-powder tablet and A solution containing silver ions is obtained by a reduction reaction.

Preferably, the polymer material is selected from the group consisting of sodium carboxymethyl cellulose, polystyrene thermoplastic elastomer, polyisobutylene, thermoplastic petroleum resin, calcium alginate. Or a combination of these.

Preferably, the total weight of the hydrophilic antibacterial film is 100 wt%, and the content of the nanosheet-silver composite is in the range of 1 to 5 wt%.

Preferably, the organic dispersant is selected from the group consisting of trisodium citrate, chitosan, polyvinylpyrrolidone or a combination thereof.

Preferably, the solution containing silver ions is selected from the group consisting of an aqueous solution of silver nitrate, an aqueous solution of silver chloride or an aqueous solution of silver bromide.

A specific example of the hydrophilic antibacterial dressing of the present invention comprises a hydrophilic antibacterial film as described above and at least one release layer, wherein the release layer is detachably covering one surface of the hydrophilic antibacterial film.

Another specific example of the hydrophilic antibacterial dressing of the present invention comprises a hydrophilic antibacterial film as described above and a waterproof backing layer, wherein the waterproof backing layer covers one surface of the hydrophilic antibacterial film. Preferably, the hydrophilic antimicrobial coating further comprises at least one release layer disposed opposite the waterproof backing layer and covering the surface of the hydrophilic antimicrobial film.

Preferably, the waterproof backing layer is a polyurethane film.

Preferably, the hydrophilic antimicrobial coating further comprises a water absorbing layer between the hydrophilic antimicrobial film and the waterproof backing layer. More preferably, the water absorbing layer is selected from the group consisting of a hydrocolloid, a hydrophilic polyurethane foam, or a combination thereof. Wherein, the hydrophilic colloid is a material which is known to absorb hydrophilic liquids (such as water or wound exudate) [eg, pectin, xanthan gum, carrageenan, carboxymethyl) Made of cellulose and its salts]. In a specific embodiment of the present invention, the hydrophilic colloid is obtained by kneading a polystyrene thermoplastic elastomer, a thermoplastic petroleum resin and sodium carboxymethylcellulose.

The invention is further described in the following examples, but it should be understood that these examples are for illustrative purposes only and are not to be construed as limiting.

Example

The following examples were prepared using the following chemicals and equipment:

[Chemical Sources and Equipment]

1. Nano-slice-silver composite: uniformly mix 38.8 mM aqueous solution of trisodium citrate (organic dispersant), 1.0 wt% aqueous solution of nano-barium and 1 mM aqueous solution of silver nitrate at a weight ratio of 1:1:1.1. And the reduction reaction is carried out for 2 to 3 hours according to the method disclosed in TW Patent Publication No. 200924878.

2. Polymer materials: sodium carboxymethyl cellulose (purchased from Ashland, model Aquasorb A-500), polystyrene thermoplastic elastomer (purchased from Kraton, model G-1657), Polyisobutylene (available from BASF, model Oppanol), thermoplastic petroleum resin (available from Eastman Chemical Company, model Eastotac H-100W) and calcium alginate (purchased from KIMICA, model ALGIN CA) .

3. Mineral oil: used as a plasticizer from Ergon, the model is Hyprene P100N.

4. Banbury mixer: purchased from ZhangJiaGang JiuYang Machinery, model X-120L.

5. Waterproof backing layer: polyurethane film (purchased from Smith & Nephew, model EU-31).

6. Release layer: purchased from 3M company, model Scotchpak 1022.

[Preparation of hydrophilic antibacterial film] <Example 1>

20 kg of polystyrene thermoplastic elastomer, 38.5 kg of thermoplastic petroleum resin and 10 kg of mineral oil were placed in a closed mixer and uniformly mixed at 130 ° C for 40 minutes at 40 rpm, at the same temperature and 1.5 kg of nano-barium-silver composite was added and mixed at a speed for 20 minutes, and then 30 kg of sodium carboxymethylcellulose was added and uniformly mixed for 20 minutes to obtain a kneaded product. The kneaded product was applied to form a hydrophilic antibacterial film having a thickness of 500 μm.

<Example 2>

The manufacturing procedure of Example 2 was the same as in Example 1 except that 30 kg of sodium carboxymethylcellulose was changed to 20 kg of sodium carboxymethylcellulose and 10 kg of calcium alginate.

<Example 3>

The manufacturing procedure of Example 3 was the same as that of Example 1 except that 20 kg of the polystyrene thermoplastic elastomer and 38.5 kg of the thermoplastic petroleum resin were changed to 40 kg of polyisobutylene and 18.5 kg of thermoplastic petroleum resin.

<Example 4>

The manufacturing procedure of Example 4 was the same as in Example 3 except that 30 kg of sodium carboxymethylcellulose was changed to 20 kg of sodium carboxymethylcellulose and 10 kg of calcium alginate.

<Example 5>

The manufacturing process of Example 5 was changed except that 38.5 kg of thermoplastic petroleum resin and 1.5 kg of nano-silver-silver composite were changed to 35 kg of thermoplastic petroleum resin and 5 kg of nano-silver-silver composite, respectively. Example 1 is the same.

[Preparation of hydrophilic antibacterial material] <Application Example 1>

The kneaded product of Example 1 was applied to a release layer to form a hydrophilic antibacterial film (silver content of 5.0 mg/100 cm ² ) having a thickness of 500 μm to prepare a hydrophilic antibacterial material of Application Example 1 ( A hydrophilic antibacterial film and a release layer detachably covering one surface of the hydrophilic antibacterial film are included.

<Application Example 2>

The kneaded product of Example 1 was applied to a waterproof backing layer to form a hydrophilic antibacterial film having a thickness of 500 μm to prepare a hydrophilic antibacterial material of Application Example 2 (containing a hydrophilic antibacterial film and covering the hydrophilic antibacterial film). a waterproof backing layer on one of the surfaces of the film).

<Application Example 3>

A hydrophilic backing layer of the application example 1 was applied to the opposite side of the release layer to prepare a hydrophilic antibacterial material of Application Example 3 (containing a hydrophilic antibacterial film and covering the hydrophilic antibacterial film). a waterproof backing layer on one surface and a release layer covering the other surface of the hydrophilic antimicrobial film).

<Application Example 4>

20 kg of polystyrene thermoplastic elastomer, 40 kg of thermoplastic petroleum resin and 10 kg of mineral oil were placed in a closed mixer, uniformly mixed at 130 ° C, 40 rpm for 45 minutes, and then added 30 kg The sodium carboxymethylcellulose was uniformly mixed for 20 minutes to obtain a kneaded product. The kneaded product was coated on the hydrophilic antibacterial film of Example 1 to form a hydrophilic colloid having a thickness of 100 to 500 μm.

A waterproof backing layer is applied on the hydrophilic colloid (opposite side opposite to the hydrophilic antibacterial film) to obtain a hydrophilic antibacterial material of Application Example 4 (containing a hydrophilic antibacterial film and covering a surface of the hydrophilic antibacterial film) a waterproof backing layer, a release layer covering the other surface of the hydrophilic antimicrobial film, and a hydrophilic colloidal water absorbing layer between the hydrophilic antimicrobial film and the waterproof backing layer).

<Application Example 5>

The manufacturing procedure of Application Example 5 was the same as that of Application Example 4 except that the hydrophilic colloid was changed to a hydrophilic polyurethane foam (available from Bayer Corporation, model Baymedix FR) (containing a hydrophilic antibacterial film, a waterproof backing layer covering one surface of the hydrophilic antibacterial film, a release layer covering the other surface of the hydrophilic antibacterial film, and a hydrophilic polyurethane between the hydrophilic antibacterial film and the waterproof backing layer Foam water absorption layer).

[antibacterial test]

The Staphylococcus aureus BCRC 10781 used in the following test is a Biosource Collection and Research Center purchased from the Food Industry Research and Development Institute (FIRDI). , BCRC) (300 Food Road, Hsinchu City, Taiwan). Pseudomonas aeruginosa is provided by the Prospective Chemical Materials and Nano Polymer Laboratory of National Taiwan University.

First, S. aureus BCRC 10781 and Pseudomonas aeruginosa were inoculated separately into Bacto nutrient broth (Difco, Cat. No. 234000) and cultured at 37 ° C for 16 to 18 hours. Then, 100 μL of the bacterial solution was taken and added to a fresh Bacto nutrient broth medium for culture for 3 hours, and then the formed bacterial culture was diluted with Bacto nutrient broth medium to a ratio of 1×10 ^{6 .} Concentration of cfu/mL. Thereafter, 100 μL of each of the diluted bacterial solutions of each strain was uniformly applied to a nutrient agar broth (15 g agar/1 L Bacto nutrient broth), and then separately A small piece of the hydrophilic antibacterial film of Example 1 was placed on the nutrient agar broth medium, Aquacel-Ag (purchased from ConvaTec, silver content 8.3 mg/100 cm ² as a positive control group), and hydrophilic water not containing silver. The colloid (produced according to the hydrocolloid procedure of Application Example 4 as a negative control group) was cultured for 24 hours, and then it was observed whether or not a zone of inhibition was produced. The results are shown in Fig. 1.

As shown in Fig. 1, compared with the non-silver-containing hydrophilic colloid, the hydrophilic antibacterial film of Example 1 and the surrounding area of Aquacel-Ag all showed obvious inhibition zones. In addition, the hydrophilic antibacterial film of Example 1, Aquacel-Ag, and the nutrient agar broth medium under the non-silver hydrophilic colloid were further taken up using a sterile cotton swab and coated on a fresh nutrient agar meat. Bacterial culture was carried out on the soup medium for 24 hours to observe whether or not there was bacterial growth. As a result of bacterial culture, it was found that when the hydrophilic antibacterial film of Example 1 and the nutrient agar broth medium under Aquacel-Ag were used for bacterial culture, no bacterial growth was observed. The situation of bacterial growth (data not shown). The above experimental results show that the hydrophilic antibacterial film according to the present invention has a remarkable antibacterial effect.

[Water absorption test]

The hydrophilic antibacterial film of Example 1 and three commercially available silver-containing dressings (Duoderm of ConvaTec, Comfeel of Coloplast Co., Ltd. and Tegasorb of 3M Company) were respectively cut into samples of 2 cm × 2 cm size and weighed (W ₀ ).

8.29 g of sodium chloride and 0.29 g of calcium chloride were uniformly dissolved in 1 L of water to obtain a saline solution, and an appropriate amount of the saline solution was placed in a petri dish. The above-mentioned four kinds of cut samples were immersed in the petri dish, and taken out after immersion for 2, 4, 6, and 28.5 hours, respectively, and weighed separately (W _t ), and the absorption rate was calculated according to the following formula. The absorption rates of the four samples were plotted against the immersion time, and the results are shown in Fig. 2.

As shown in FIG. 2, the hydrophilic antibacterial film of Example 1 has a significantly higher absorption rate at each time point than the other three commercially available silver-containing dressings, and can reach an absorption rate higher than 300% after 6 hours of immersion, showing The water absorption (including water absorption and water absorption rate) of the hydrophilic antibacterial film of the present invention is significantly superior to those of the commercially available dressings.

In addition, the hydrophilic antibacterial dressing of the present invention has no obvious cytotoxicity, has high biocompatibility, and has a low silver content (about 5.0 mg/100 cm ² ), which can reduce the manufacturing cost.

In summary, the hydrophilic antibacterial film of the present invention is more antibacterial and absorbent than the non-silver-containing hydrophilic colloid and the commercially available silver-containing dressing, so that the wound is in a good healing environment and the nano-silver particles can be effectively dispersed. In the hydrophilic antibacterial film, it has high biocompatibility and low manufacturing cost.

The above is only the preferred embodiment and the specific examples of the present invention, and the scope of the invention is not limited thereto, that is, the simple equivalent change according to the scope of the invention and the description of the invention. And modifications are still within the scope of the invention patent.

Figure 1 is a photograph showing the antibacterial effect of the hydrophilic antibacterial film, Aquacel-Ag and a non-silver-containing hydrophilic colloid of the present invention on Staphylococcus aureus and Pseudomonas aeruginosa;

Figure 2 is a graph of absorbance versus penetration time illustrating the absorption of the hydrophilic antimicrobial film of the present invention with a commercially available silver-containing dressing for penetration time.

Claims (10)

A hydrophilic antibacterial film obtained by kneading a nano-powder-silver composite with a polymer material, wherein the nano-powder-silver composite is composed of an organic dispersant, a nano-powder tablet and a A solution containing silver ions is obtained by a reduction reaction. The hydrophilic antibacterial film according to claim 1, wherein the polymer material is selected from the group consisting of sodium carboxymethyl cellulose, polystyrene thermoplastic elastomer, polyisobutylene, and thermoplastic petroleum resin. Calcium alginate or a combination of these. The hydrophilic antibacterial film according to claim 1, wherein the hydrophilic antibacterial film has a total weight of 100 wt%, and the nanoquinone-silver composite has a content ranging from 1 to 5 wt%. The hydrophilic antibacterial film according to claim 1, wherein the organic dispersing agent is selected from the group consisting of trisodium citrate, chitosan, polyvinylpyrrolidone or a combination thereof. The hydrophilic antibacterial film according to claim 1, wherein the silver ion-containing solution is selected from the group consisting of an aqueous solution of silver nitrate, an aqueous solution of silver chloride or an aqueous solution of silver bromide. A hydrophilic antibacterial material comprising a hydrophilic antibacterial film according to any one of claims 1 to 5, and at least one release layer, wherein the release layer is detachably covering the hydrophilic antibacterial film. a surface. A hydrophilic antibacterial material comprising a hydrophilic antibacterial film according to any one of claims 1 to 5, and a waterproof backing layer, wherein the waterproof backing layer covers a surface of the hydrophilic antibacterial film. The hydrophilic antibacterial dressing according to claim 7, wherein the waterproof backing layer is a polyurethane film. The hydrophilic antibacterial dressing according to claim 7, wherein the hydrophilic antibacterial dressing further comprises a water absorbing layer between the hydrophilic antibacterial film and the waterproof backing layer. The hydrophilic antibacterial dressing according to claim 9, wherein the water absorbing layer is selected from the group consisting of a hydrophilic colloid, a hydrophilic polyurethane foam, or a combination thereof.