TW201313204A - Dental implant with hydrophilic moisturized coating and method for manufacturing the same - Google Patents

Abstract

The present invention relates to a dental implant on which a hydrophilic water-retaining layer is coated, and a manufacturing method thereof. More specifically, the dental implant in which a hydrophilic water-retaining layer is coated on the surface thereof, according to the invention, improves the blood protein adhesion ability and hemocompatibility of a dental implant, thereby enabling effective osseointegration. Therefore, the invention can be used for the manufacture of an implant having improved osseointegration while imparting hydrophilic surface properties by preventing the hydrophobization of the surface of the implant.

Description

Dental implant coated with hydrophilic moisturizing film and manufacturing method thereof

The present invention relates to a dental implant having a surface coated with a hydrophilic moisturizing film and a method of producing the same.

Dental implants (hereinafter referred to as implants) are artificial teeth that can be used to permanently replace a defective tooth, and are required to be functionally capable of performing the function of the tooth. In addition, it is possible to manufacture a load that can be appropriately dispersed and applied to the teeth during chewing, and can be used for a long period of time, and it is also required to be finely made in appearance so as to have a form and a color which are not much different from the actual teeth.

The implant is transplanted into the living tissue in the oral cavity, that is, in the alveolar bone and fixed. After the implantation in the body, the metal of the metal implant is contacted and rubbed by the body fluid and the body fluid or the body tissue. The ions are dissolved, thus corroding the implant. In addition, metal ions eluted from metal implants damage macrophages in the body or invade into somatic cells, which cause inflammatory cells and giant cells. Therefore, the implant has excellent biocompatibility.

This implant material has been developed using a variety of metals and alloys, but titanium metal and its alloys which have high biocompatibility, high mechanical strength and bio-inertity to human body tissues are currently in development.

On the other hand, a method of increasing the surface roughness of the implant and increasing the variable area in contact with the living tissue is used, thereby making the implant stable in bone binding in the living body. A typical treatment method for increasing the surface area of the implant - Sandblasting with large grit and acid treatment (SA method) is to blast the alumina (Al 2 O 3 ) particles on the surface of the implant to produce pits ( The method of treatment with strong acid (H2SO4/HCl) after crater and micro-pit increases the surface area by more than 40% compared with the conventional resorbable blasting media (RBM), so that after particle spraying The implants produced by the acid treatment method reduced the average healing time after implantation from 12 weeks to 6-8 weeks.

However, the surface of the hydrophilic titanium surface-treated by the SA method has a drawback of being rapidly hydrophobized due to irreversible absorption of a carbon contamination source in the air. The hydrophobized surface prevents the infiltration of bone cells into the surface of the implant, and reducing the contact rate between the bone and the implant from the early stage of planting may be a potential reason for the failure of the initial implant surgery.

Therefore, countermeasures against the hydrophobicity of the titanium implant surface manufactured by the SA method in the atmosphere to maintain hydrophilicity are proposed, and a representative example is that the titanium implant is packaged in a container filled with water or an inert gas, thereby blocking the air. s contact.

The packaging method as described above is quite effective in maintaining the hydrophilicity of the surface of the titanium implant, but the recent development of the implant may be accompanied by limitations in use. That is, a major mainstream of recent implant development is to coat the surface of the implant with chemicals, peptides or proteins that promote osseointegration, thereby shortening the time during the implantation operation, when the implants coated with these substances are wrapped with water. What effect on the coating is not verified, and if it has a detrimental effect, other packaging media that can solve the problem are needed.

Therefore, it is necessary to develop a new type of plant that can prevent the hydrophobization of the surface of the implant, thereby maintaining the hydrophilic surface characteristics, improving the blood affinity, and improving the osseointegration performance at the initial stage of planting, from the concept that the packaging method of the inert environment is completely different. body.

An object of the present invention is to provide a dental implant capable of maintaining hydrophilicity imparted to the surface of a titanium implant by the SA method to form a hydrophilic moisturizing film, and a method for producing the same.

The present invention provides a dental implant having a rough surface comprising a titanium or titanium alloy material; and a dental implant in the dental implant that forms a hydrophilic moisturizing film that maintains the hydrophilicity of the surface and maintains a wet state at a normal temperature. body.

In addition, the present invention also provides a method of manufacturing a dental implant comprising the steps of: forming a rough surface on a dental implant surface of titanium or titanium alloy; removing organic substances present on the surface of the rough dental implant. a step of contaminating the source; and a step of applying the surface of the dental implant from which the organic contamination source is removed to a hydrophilic moisturizing film at a normal temperature and maintaining a wet state.

DETAILED DESCRIPTION OF THE INVENTION A dental implant according to a preferred embodiment of the present invention and a method of preparing the same are as follows.

First, the term "implant" as used in this specification refers to a substitute for human tissue that can recover loss. Dental implants usually refer to a fixture embedded in the alveolar bone after the natural tooth root is detached. In place of the tooth root of the lost tooth, the artificial tooth is fixed on the upper part of the tooth to restore the original function of the tooth.

In particular, the surface of the dental implant in this specification can be interpreted as the surface of the fixture associated with the alveolar bone. Specifically, the dental implant of the present invention may be made of titanium or contain aluminum, One or more of ruthenium, osmium, vanadium, zirconium, platinum, and magnesium are formed of a titanium alloy of titanium.

The nature of the implant surface is an important factor in the process of osseointegration of new bone tissue on the surface of the implant. The stability of the implant in the light tissue is decisively determined by the interaction with the surface of the implant and the surrounding tissue and the morphology of the implant. For example, the implant surface can be altered to improve the fixation of the implants of the light tissue and the mutual solubility of the implants, and strengthen the osseointegration of the implants to accelerate the healing process. The osseointegration of the implant can be improved by processing the surface of the implant, and a rough method can be used on the surface of the implant. The roughness can generally be formed in micrometer units. For example, the roughness of the micro unit is formed on the surface of the implant in the range of 0.1-50 μm or in the range of 1-30 μm.

Such micron unit roughness can be utilized, for example, selected from the group consisting of grit blasting, resorbable blasting media, acid etching, alkali etching, titanium plasma spraying ( Titanium plasma spray), sandblasting with large grit and acid treatment, two-stage oxidation (anodizing), and laser surface processing.

As described above, the surface of the implant having a micron unit roughness after roughening has an advantage of improving the osseointegration of the implant due to an increase in its surface area, but may have an increase in air due to a large surface area during storage until the implantation operation. Disadvantages of pollution from various sources of pollution. In particular, for example, a carbon contamination source existing in air such as carbon dioxide or organic carbon is adsorbed irreversibly on the surface of the implant, and the surface of the implant can be hydrophobic. When the surface of the implant is hydrophobized due to the contamination of the contaminated source, various proteins existing in the blood when transplanted into the living body cannot adhere to the surface of the implant to reduce the adaptability, thereby causing a problem of osseointegration, and may also induce a source of contamination. The inflammatory reaction caused.

Therefore, it is necessary to remove the organic pollution source on the surface of the implant, and the method for removing the organic pollution source is, for example, a method of irradiating ultraviolet rays to the surface of the implant having a micron unit roughness, a method using plasma, an etching method, etc., among which it is preferably used. A simple method of irradiating ultraviolet light.

According to an embodiment of the present invention, the surface of the dental implant forms a hydrophilic moisturizing film which maintains a wet state at normal temperature, thereby preserving hydrophilicity.

The surface of the implant after completely removing the pollution source by the ultraviolet irradiation method forms a hydrophilic moisturizing film, which can improve the affinity with blood after transplanting the implant, thereby improving various blood proteins (for example, inducing bone binding) provided by the blood. Affinity of protein, etc., ultimately leads to The result of osseointegration of high implants.

According to a specific embodiment, the hydrophilic humectant film is formed by using a solution containing a sugar alcohol, which may be selected from the group consisting of glycerin, sorbitol, xylitol, mannitol, maltitol, and lactitol. At least one, the hydrophilic moisturizing film can be formed by immersing the dental implant in a hydrophilic moisturizing film.

Here, when the total amount of the sugar alcohol contained in the solution is at least 15% by weight, the hydrophilic moisturizing film can be maintained for a long period of time.

In addition, the solution may further contain a functional biomaterial selected from the group consisting of oligochitosan, oligomeric hyaluronic acid, collagen, and gel, thereby further enhancing antibacterial function, bone formation, and Bone binding efficacy.

The dental implant formed with the hydrophilic moisturizing film according to the present invention and the method for producing the same improve the blood affinity of the implant, thereby making effective bone bonding possible, thereby improving the success rate of the implantation operation and shortening the operation period Effect.

The following is explained in detail by more than one specific embodiment. However, these examples are for illustrative purposes only, and the scope of the invention is not limited thereby.

Example 1: Coating of carbonaceous contamination source on the surface of the implant

The surface of the titanium component implant is usually manufactured by mechanical processing using an automatic rotary disk, in which case the surface average roughness is about 0.2 μm. The average roughness of the implant surface was adjusted to about 20 μm by acid treatment (SA method) after the implant surface particles were sprayed.

Thereafter, the rough-formed implants were irradiated with ultraviolet rays for 10 minutes to remove the carbon contamination source, the plants from which the carbon contamination source was removed were immersed in a 50% by weight glycerin solution, and glycerin was uniformly coated on the fixed portion of the implant.

At this time, the implant for the normal aging test and the accelerated aging test were maintained under the same solution temperature, ambient temperature, solution concentration, etc., to form a glycerin humectant film.

Further, a normal aging test and an accelerated aging test implant using a glycerin solution further containing 5% by weight of oligochitosan to form a moisturizing film were prepared.

The glycerin-coated implants manufactured as above were used in the following Examples 2 and 4.

Example 2: Blood protein adhesion of normally aged glycerin moisturizing film-coated implants and Blood affinity test

The implants coated with the glycerin solution and the glycerin solution containing the oligochitosan solution prepared in the above-mentioned Example 1 for normal aging experiments were respectively placed at room temperature (about 25 ° C) for 2 weeks. After 4 weeks, it was immersed in a solution containing blood proteins (albumin, globulin, serum), and then the extent of blood proteins attached to the surface of the implant was observed.

At this time, the control group used an implant that did not coat the glycerin solution but cleared the carbon contamination source. In the comparative experimental group, the implanted distilled water and the carbon contamination source were removed and the surface of the implant from which the carbon contamination source was removed was uniformly coated with the HEPES buffer solution. body.

As shown in Fig. 1, the implant coated with the glycerin solution and the implant coated with the glycerin solution containing oligochitosan maintained the adhesion energy of blood proteins even after aging for 4 weeks.

In contrast, coating of HEPES known to have excellent moisture retention ability reduces the adhesion of blood proteins over time.

Here, in the graph of Fig. 1, CC denotes an implant which removes a carbon contamination source, DIW denotes distilled water, GC denotes glycerin, and OCS denotes oligochitosan.

Further, the implants placed at normal temperature for 4 weeks were each immersed in blood, and blood affinity was observed.

As shown in Fig. 2, in the implant coated with the glycerin solution and the implant coated with the oligochitosan-containing glycerin solution, the blood penetrates into the upper portion of the fixed portion of the implant, so that blood affinity can be confirmed with the naked eye. The sex is high, while the HEPES-coated implants have a relatively low blood affinity observed by the naked eye.

Example 3: Blood protein adhesion energy and blood affinity test of an implant coated with an accelerated aged glycerin moisturizing film.

The glycerin-coated implants prepared in Example 1 and the glycerin solution containing the oligochitosan solution were each placed under high temperature drying conditions (55 ° C) for 3 weeks. This is equivalent to an aging time of about 6 months when converted to normal aging time at normal temperature. After the sputum, the implants were each immersed in a solution containing the same blood protein as in Example 2, and the degree of blood protein attached to the surface of the implant was observed.

At this time, the control group used an implant that did not have a carbon contamination source, and the control group used an implant that did not coat the glycerin solution to remove the carbon contamination source.

As shown in Fig. 3a and Fig. 3b, even if the surface is aged for 3 weeks under high temperature drying accelerated aging conditions, the surface color of the dark gray can be maintained due to the wet state, and it is confirmed that the coating is sweet. The implant of the oil solution and the implant coated with the glycerol solution containing the oligochitosan can maintain the adhesion energy of the blood protein.

On the contrary, after the implants which were not coated with the glycerin solution and the carbon contamination source were removed under accelerated aging conditions, it was confirmed that the adhesion energy of the protein almost disappeared.

In addition, blood affinity was observed by immersing the implants placed under high temperature for 3 weeks in the blood.

As shown in Fig. 4, the implant coated with the glycerin solution and the implant coated with the glycerol solution containing the oligochitosan were able to confirm the same high blood affinity as in Example 2, and the glycerin solution was not observed by the naked eye. The blood affinity of the implants that remove carbon sources is clearly not very high.

Example 4: Comparative experiment of releasing torgue of an implant that maintains or removes a moisturizing membrane

According to Example 2 and Example 3, in the normal aging test and the accelerated aging test mode, it was confirmed that the implant coated with the glycerin solution and the implant coated with the oligochitosan-containing glycerin solution can well maintain the blood protein. Adhesion energy and blood affinity.

However, since Example 2 and Example 3 are in-vitro experiments, in order to verify that the implant actually coated with the glycerin solution and the implant coated with the oligochitosan-containing glycerin solution are implanted into the human body, Whether to play the same effect, you need to do an in-vivo experiment. That is, it is necessary to verify whether or not the moisturizing film on the surface of the implant functions as a hindrance factor for bone bonding.

In Example 4, the implant coated with a solution containing 10% by weight, 25% by weight, and 50% by weight of glycerin was allowed to stand under high temperature conditions (about 55 ° C) for 80 days and 120 days. This is equivalent to an aging time of about 2 years and 3 years when converted to normal aging time at normal temperature.

The implant undergoing this accelerated aging process is transplanted to the alveolar bone of the miniature pig. At this time, the control group used the implant (SA) which did not remove the carbon pollution source, and the comparative experiment group transplanted into the alveolar bone of the miniature pig using the SA implant (CC) which was not coated with the glycerin solution and cleared the carbon contamination source.

Figure 5 is a graph showing the release torque measured after removal of the implant for 16 days of osseointegration time after implantation. The higher the release torque, the better the bone binding of the implant.

As shown in Fig. 5, the coating of the moisturizing film is dried in an amount of 10% by weight in the implant (CC, aging for 2 years) and accelerated aging for 2 years, except for the removal of the carbon source, after the accelerated aging process. In addition to the glycerol implant (CC+GC10, aged for 2 years), the implants coated with the humectant film containing 25% by weight or more of glycerin have the same level as the implant (CC) from which the carbon contamination source is removed. Loosen the torque.

Based on the above experimental results, the system analyzes the release torque between the implants containing 10% by weight, 25% by weight, and 50% by weight of glycerin, and it is possible to obtain a solution containing at least 15% by weight or more of glycerin. Coating the implant ensures an effective hydrophilic humectant film performance.

Therefore, it was confirmed that the moisturizing film formed by coating the glycerin solution can maintain the blood protein adhesion energy and blood affinity for the implant for a long period of time, and does not function as a barrier element of osseointegration.

Fig. 1 is a result of evaluation of blood protein adhesion energy of a moisturizing film-coated implant of a specific example at normal temperature (where CC: carbon removal, DIW: deionized water, HEPES: organic chemical buffer) , GC: glycerol, OCS: oligochitosan, GC/OCS: glycerol/oligomeric chitosan); Fig. 2 is the blood of a humectant-coated implant in a specific embodiment which is normally aged at normal temperature. Evaluation results of affinity; Figures 3a and 3b are evaluation results of blood protein adhesion energy of a moisturizing film-coated implant according to a specific example of accelerated aging at 55 ° C; Figure 4 is at 55 ° C Accelerated aging - evaluation of blood affinity of a humectant-coated implant of a specific embodiment; and Figure 5 is accelerated aging at 55 ° C. A specific embodiment of a humectant-coated implant is planted to a micro Animal test results of the release torque after the osseointegration of the pigs (GC10: 10% by weight glycerol, GC25: 25% by weight glycerol, GC50: 50% by weight glycerol).

Claims (11)

A dental implant coated with a hydrophilic moisturizing film, comprising: a dental implant having a rough surface of titanium or titanium alloy; and forming and preserving hydrophilicity of the surface on the surface of the dental implant, A hydrophilic moisturizing film that maintains a wet state at normal temperature. A dental implant coated with a hydrophilic moisturizing film according to claim 1, wherein the hydrophilic moisturizing film is formed of a solution containing a sugar alcohol. A dental implant coated with a hydrophilic moisturizing film according to claim 2, wherein the sugar alcohol is selected from the group consisting of glycerin, sorbitol, xylitol, mannitol, maltitol and lactitol At least one of the groups. A dental implant coated with a hydrophilic moisturizing film according to claim 3, wherein the total amount of the sugar alcohol contained in the solution is at least 15% by weight. A dental implant coated with a hydrophilic moisturizing film according to claim 2, wherein the solution further comprises a component selected from the group consisting of oligomeric chitosan, oligomeric hyaluronic acid, collagen and gel. The substance in the group. A method for producing a dental implant coated with a hydrophilic moisturizing film, the method comprising the steps of: forming a rough surface on a dental implant surface of titanium or titanium alloy; removing the dental implant forming the roughness a step of presenting an organic contamination source on the surface of the body; and a step of applying the surface of the dental implant from which the organic contamination source is removed to a hydrophilic humectant film maintained at a normal temperature. A method for producing a dental implant coated with a hydrophilic moisturizing film according to claim 6, wherein the hydrophilic moisturizing film is formed by dipping the dental implant into a solution containing a sugar alcohol. . Preparation of a dental implant coated with a hydrophilic moisturizing film according to claim 7 of the patent application scope The method, wherein the sugar alcohol is at least one selected from the group consisting of glycerin, sorbitol, xylitol, mannitol, maltitol and lactitol. A method for producing a dental implant coated with a hydrophilic moisturizing film according to claim 8, wherein the total amount of the sugar alcohol contained in the solution is at least 15% by weight. A method for producing a dental implant coated with a hydrophilic moisturizing film according to claim 7, wherein the solution further comprises an oligochitosan, an oligomeric hyaluronic acid, a collagen and a condensed solution. The substance in the group consisting of glue. A method for producing a dental implant coated with a hydrophilic moisturizing film according to claim 6, wherein the roughening of the surface of the dental implant can utilize a particle blasting method or an absorbent jet medium. Resorbable blasting media, acid etching, alkali etching, titanium plasma spray, sandblasting with large grit and acid treatment, two-stage Formed by anodizing or laser surface processing.