TW201142028A

TW201142028A - Stable self-luminous vector dedicated to Gram-negative bacteria and manufacturing method thereof

Info

Publication number: TW201142028A
Application number: TW99116242A
Authority: TW
Inventors: Zheng-Xun Qiu; qi-liang Chen; yao-guang Huang
Original assignee: Zheng-Xun Qiu; qi-liang Chen; yao-guang Huang
Priority date: 2010-05-21
Filing date: 2010-05-21
Publication date: 2011-12-01
Also published as: TWI414599B

Abstract

A stable self-luminous vector dedicated to Gram-negative bacteria comprises luxABCDE self-luminous gene, a promoter for regulating the luxABCDE self-luminous gene, ColE1 replicon, a gene resistant to ampicillin and kanamycin, genes associated with stabilizing plasmid, and genes associated with conjugation, such that this invention can be easily introduced by conjugation into bacteria to express luminous gene proteins and exist in large amount in the bacteria to consistently illuminate with high performance without additional luminous substrate and screening antibiotics, thereby allowing convenient dynamic observation of bacteria in living animals. The present invention is prepared by the following steps: a. obtaining a plasmid capable of regulating the luminous gene; b. introducing ColE1 replicon and resistant gene plasmid into plasmid pSE34 by ligation; and c. using steps a and b to obtain the plasmid of this invention.

Description

201142028 六、發明說明：【發明所屬之技術領域】 « • 本發明係一種可用於標定細菌的發光質體及其製造方法，尤指技術上提供一種在細菌世代繁殖中可穩定且大量存在於細菌中的革蘭陰性細菌之自發發光載體及其製造方法。【先前技術】 • “ 般欲仵知細菌.在動物體中的分布和行為，大多是在實驗動物犧牲後，才能分析動物器官標本，而不能直接觀察。 ;疋遂有人研發利用發光基因在細菌中表現的方法，來觀察細菌在動物體中的分布和行為，而利用發光基因在細菌中表現的方法，一般來講有三種主要途徑：鲁1.在質體中直接表現發光基因，但受限於質體本身的不穩 J性’以致大部份的細菌容易遺失所植入的質體，無法穩定且大量的存在細菌中，而在細菌的繁殖中，質體難以穩定的存在於各個子細菌，往往只能維持少數幾代而已，因此整體細菌表現的強度很快即轉弱。若使用抗生素筛選，強迫細菌保有該質體，否則無法存活，但此法卻無法運用於活體動物中’因為如果給動物餵食抗生素，則由抗生素對測試動物所引起的變因將可能複雜化實驗結果。 2.將發光基因藉由轉位子（transpQSGn)逢機地插人細菌的 201142028 染色體中’但不能轉定被插入的DNA處是否為重要的基因所在，又因轉位發生機*座尤古 —,. 货生機羊不呵，更增填篩選上的困難度。況且此法只針對該種已轉位的菌#，若應用於其它種菌株則其轉位子之位置將會不同’如此將更增填變因，以致不能作多種菌株之觀察和比較。 3·以雙重組交換將發光基因插入細菌的染色體中，雖缺可以準確地插人特定基时，但其發生機率以，又因發光基因（μ歷如）本身很長（至少7kb)，更降低重組交換的機率，難以完成大分子歸在細菌中的選殖及傳遞。是以，針對上述習知結構所存在之問題點，如何開發 -種更具理想實用性之創新結構，實消費者所殷切企盼，亦係相關業者須努力研發突破之目標及方向。有鑑於此，發明人本於多车你宜多年從事相關產品之f造開發與设計經驗，針對上述之目栌，4 ^ ^ 標洋加設計與審慎評估後，終得一確具實用性之本發明。【發明内容】八欲解決之技術問題點：一般欲得知細菌在動物體中的 :佈和行為’大多需犧牲動物才能分析動物器官標本，而一般利用發光基因在細菌中表現的方法，冑以下幾點缺失轉1.若在質體中直接表現發光基因，《限於質體本身的不穩定性，大部份的細菌容易遺失所植入的質體，…定且大量的存在…；2.若發光基因 J子 201142028 (transp〇s〇n)逢機地插入細菌的染色體，不能確定被插入 DNA處是否為重要的基因所在，且轉位發生機率不高；另外也只受限於該轉位之測試菌株可被使用而e，卻不能廣泛地應用於其它多㈣株上m重組純將發光基因插入細菌的染色體中’其發生機率不冑，轉光基因本身很長不容易完成在細菌中的選殖及傳遞。201142028 VI. Description of the invention: [Technical field to which the invention pertains] « The present invention is an illuminating plastid which can be used for calibrating bacteria and a method for producing the same, and in particular to provide a technique which is stable in the generation of bacteria and is abundantly present in bacteria. A spontaneous luminescent carrier of Gram-negative bacteria and a method of producing the same. [Prior Art] • “The general desire to know bacteria. The distribution and behavior in animals. Most of the animals can be analyzed after the sacrifice of the experimental animals, and can not be directly observed. 疋遂 Some people develop luminescent genes in bacteria The method of performance, to observe the distribution and behavior of bacteria in the animal, and the use of luminescent gene expression in bacteria, generally there are three main ways: Lu 1. Direct expression of luminescent genes in the plastid, but subject to Limited to the unstable J nature of the plastid itself, most of the bacteria are prone to lose the implanted plastids, and cannot be stably and abundantly present in the bacteria. In the reproduction of bacteria, the plastids are difficult to exist stably in each Sub-bacteria can only be maintained for a few generations, so the intensity of the overall bacterial performance will soon weaken. If antibiotics are used to screen, the bacteria are forced to retain the plastid, otherwise they cannot survive, but this method cannot be applied to living animals. 'Because if the animal is fed antibiotics, the cause of the test animal caused by antibiotics may complicate the experimental results. By transposing the transposon (transpQSGn) into the bacteria on the 201142028 chromosome, it is not able to determine whether the inserted DNA is the important gene, and because of the translocation machine, the Ukrainian--. No, it will increase the difficulty of screening. Moreover, this method only targets the bacteria that have been translocated. If it is applied to other strains, the position of the transposon will be different. Therefore, it is not possible to observe and compare a variety of strains. 3. Double-group exchange of luminescent genes into the chromosome of bacteria, although the lack of can accurately insert a specific base, but its probability of occurrence, but also due to luminescent genes (μ calendar ) itself is very long (at least 7kb), which reduces the probability of recombination exchange, and it is difficult to complete the selection and transmission of macromolecules in bacteria. Therefore, in view of the problems of the above-mentioned conventional structures, how to develop - more The innovative structure of ideal practicability is eagerly awaited by consumers, and it is also the goal and direction of relevant industry players to work hard to develop breakthroughs. In view of this, the inventor should have been engaged in the manufacture of related products for many years. With the design experience, in view of the above-mentioned objectives, 4 ^ ^ standard Yangjia design and prudent evaluation, the invention has finally become practical. [Summary of the invention] Eight technical problems to be solved: generally want to know Bacteria in the animal body: cloth and behavior 'mostly need to sacrifice animals to analyze animal organ specimens, and generally use the method of luminescent gene expression in bacteria, 胄 the following points are missing. 1. If the luminescent gene is directly expressed in the plastid "Limited to the instability of the plastid itself, most of the bacteria are prone to lose the implanted plastid, ... and a large number of existence...; 2. If the luminescent gene J 201142028 (transp〇s〇n) Insertion of the chromosome of the bacteria, can not determine whether the gene inserted into the DNA is important, and the probability of translocation is not high; in addition, the test strain limited to the translocation can be used, but can not be widely applied. On other (four) strains, m recombinantly inserted the luminescent gene into the chromosome of the bacteria. The probability of occurrence was not good, and the transgenic gene itself was long and difficult to complete the selection and transmission in bacteria.

解决問題之技術特點：一種革蘭陰性細菌專用之穩定自發發光載體，其係包含厶⑽自發發光基因可啟動自發發光基因之啟動子、c〇1E1複製子、對氨比西林與卡那霉素之抗性基因、心及穩定質體之相關基因，以及至與^^ 、接合生殖作用相關的基因；本發明之質體製備步驟如下a.獲得一可啟動發光基因之質體； b_將含ColEl複製子及抗性基因之質體接入質體pSE34中 ;c.結合步驟a.與b.質體製得本發明之質體。對照先前技術之功效：本發明之革蘭陰性細菌專用之穩定自發發光載體有以下幾點功效： ’可直接使用此發光質體於細菌中，有利於觀察其在動物體内的分佈變化。因而無需犧牲動物，即可直接分析研究細菌在動物體内的分佈和行為。第二，本發明之發光質體可穩定存在於細菌中，並可大里複製質體，如此有助於細菌的發光強度，能持續而且 201142028 能高效地表現，因而能去ΒΒ哲跑^Technical characteristics of solving the problem: a stable spontaneous luminescent vector for Gram-negative bacteria, which comprises a promoter of 厶(10) spontaneous luminescent gene-initiating spontaneous luminescent gene, c〇1E1 replicon, and ampicillin and kanamycin a gene related to the resistance gene, the heart and the stable plastid, and a gene related to phlegm and zygosity; the plastid preparation step of the present invention is as follows: a plastid to obtain a luminescent gene; b_ The plastid containing the ColEl replicon and the resistance gene is inserted into the plastid pSE34; c. The plastid of the present invention is obtained by combining steps a. and b. In contrast to the efficacy of the prior art: the stable spontaneous luminescent vector for the gram-negative bacteria of the present invention has the following effects: 'The luminescent plastid can be directly used in bacteria to facilitate observation of its distribution in the animal. Therefore, the distribution and behavior of bacteria in animals can be directly analyzed without sacrificing animals. Secondly, the luminescent body of the present invention can be stably present in bacteria, and can reproduce the plastids in a large amount, so that the luminescence intensity of the bacteria can be sustained, and the 201142028 can be efficiently expressed, so that it can go to the ΒΒ 跑 Run ^

Sb克服質體在細菌的繁殖中，難以穩定且大量的存在細菌中的缺點。第三’本發明可利用質體之接人按0生殖作用，既可幫助選殖（c 1 oni ng)又可便於續晳駚六Λ社更於°亥質體在細菌與細菌之間傳'遞本發明之發光質體。如此即可冑IΜ ^ 4 π | j間早的選出所需要的高效能且持續發光的細菌，而可減少_吝Λ，s J汗多在選殖（cloning)時篩選工作的負擔，尤其本發明因含右碎炙舌乃口 3有迕夕重要功能之基因以致質體DMA過大，所以當在一般dna准祐r 1 .、田你奴⑽A選殖（cloning)的剪接上，成功機率相對上很低。因此，南丨田你人丄+ & u此利用接合生殖作用可降低選殖上的困難度。有關本發明所採用之技術、手段及其功效，茲舉一較佳實施例並配合圖式詳細說明於后，相信本發明上述之目的、構造及特徵，當可由之得一深入而具體的瞭解。【實施方式】請參閱第四圖，本發明之革蘭陰性細菌專用之穩定自發發光質體pSE-Luxl(SEQIDNOl)，含有至少一自發發光基因（SEQ ID NO 21)、至少一可啟動自發發光基因之啟動子、至少一可大量複製的C〇lEl複製子、至少一抗性基因、至少一基因（SEq IDN〇3)、至少一仰％基因（SEQ ID NO 4)、至少一仰斤基因（SEQ ID NO 5)、至少一以Μ基因（SEQ ID NO 6)與至少一基因（SEQ ID NO 7)，以及包含至少一基因（SEQ ID NO 8)、至少一基因 201142028 (SEQ IDN09)、至少一 W/iV 基因（SEQ IDNOIO)、至少一基因（SEQ ID NO 11)、至少一沒基因（SEQ ID NO 12)、至少Sb overcomes the shortcomings of bacteria in the reproduction of bacteria, which are difficult to stabilize and are abundant in bacteria. The third 'the invention can use the plastid to receive the 0 reproductive function, which can help the colonization (c 1 oni ng) and can be easily renewed. The 质 Λ 更更 ° ° ° ° ° ° ° ° ° 在在在'Handing the luminescent body of the invention. In this way, 胄IΜ ^ 4 π | j can be selected early to select the high-efficiency and continuous luminescent bacteria, and can reduce the burden of screening work during cloning, especially The invention is based on the gene containing the important function of the right-handed tongue and the mouth 3, so that the plastid DMA is too large, so when the general dna quasi-r 1 ., Tian Youru (10) A cloning splicing, the probability of success is relatively high. It is very low. Therefore, Nantiantian you 丄+ & u use zygosity to reduce the difficulty of colonization. The above-mentioned objects, structures and features of the present invention will be described in detail with reference to the preferred embodiments of the present invention. . [Embodiment] Please refer to the fourth figure, the stable spontaneous luminescent plastid pSE-Luxl (SEQ ID NOl) for Gram-negative bacteria of the present invention, comprising at least one spontaneous luminescent gene (SEQ ID NO 21), at least one primable spontaneous luminescence a promoter of the gene, at least one C〇lEl replicon which can be largely replicated, at least one resistance gene, at least one gene (SEq IDN〇3), at least one % gene (SEQ ID NO 4), at least one gene (SEQ ID NO 5), at least one gene (SEQ ID NO 6) and at least one gene (SEQ ID NO 7), and at least one gene (SEQ ID NO 8), at least one gene 201142028 (SEQ ID NO), At least one W/iV gene (SEQ ID NOIO), at least one gene (SEQ ID NO 11), at least one gene (SEQ ID NO 12), at least

一；?///7 基因（SEQ IDNO 13)、至少一；?///沒基因（SEQ IDNO 14) 、至少一 p"/夕基因（SEQ ID NO 15)、至少一基因（SEQa ??///7 gene (SEQ ID NO 13), at least one; ?/// no gene (SEQ ID NO 14), at least one p" / eve gene (SEQ ID NO 15), at least one gene (SEQ

ID NO 16)、至少一基因（SEQ ID NO 17)、至少一 taxA 基因（SEQ ID NO 18)、至少一基因（SEQ ID NO 19)與至少一 iaxC 基因（SEQ ID NO 20)。其中，該可啟動自發發光基因之啟動子為 PlacZ啟動子（SEQ ID NO 22)。其中，該抗性基因可為對氨比西林（amp i c i 11 i η，Amp) 之抗性基因或對卡那霉素（kanamycin，Kan)之抗性基因，或含有對氨比西林（amp i c i 11 i η，Amp)之抗性基因加上對卡那霉素（kanamycin，Kan)之抗性基因兩種抗性基因。矣中，該pjr、parG ' parF、stbD與stbE為穩定質後之相關基因；該 pi///、p///心 Ρ/ΛΤ5、、pi 1X8、pi 1X9、pi 1X10、pi 1X11、taxA、taxB 與 taxC 因為接合生殖相關的基因，有助於質體的接合生殖作用，幫助質體的選殖以及在細菌與其他菌種細菌間的傳遞（如將質體從沙門氏菌傳遞至大腸桿菌）。其中，/z/W万自發發光基因既含有發光酶万基因又有脂肪酸還原酶基因，即可合成脂肪醛，直接供發光酶於發光時所需的受質，因此無需額外受質（如脂肪 201142028 ^ 真加。本發明之質體pSE-Luxl可表現自發發光基因蛋白質，可非常穩定的存在細菌中持續發光，在細菌世代繁殖中可穩#旦++ 令 L疋且大量存在於細菌中，更有利於活體動物内直接觀察。本發明之革蘭陰性細菌專用之穩定自發發光載體制備步驟如下： (a) 、將缺乏啟動子的發光基因（接入一含啟動子的質體中，使發光基因（/ 及αβ)可利用啟動子來啟動轉錄。 (b) 將含至少一 ColEl複製子及至少一抗性基因之革蘭陰性細菌質體接入質體pSE34中。 (c) 、將步驟（a)所構築之質體接入步驟（b)所構築之質體中以製得該革蘭陰性細菌專用之穩定自發發光載體。〔實施例〕請參閱第一至第三B圖，本發明實際在製備革蘭陰性細菌專用之穩定自發發光載體的詳細製備步驟如下： (a)、構築質體p3ZLux4 ( 1 〇 ):將缺乏啟動子的發光基因從質體pXen-5中以限制酶切出，並接入含PucZ啟動子的質體PGEM3-Zf +令，使發光基因 (/仏可利用質體pGEM3-Zf+中的PUcZ啟動子來啟動轉錄。其中，質體pXen-5為Xenogen公司之商品化的質體 201142028 (Alameda，CA，USA)，有 18, 357 個鹼基對（bp)。含對卡那霉素（kanamycin，Kan)之抗性基因及/仏之自發發光基因。但缺啟動子來啟動，以致不能啟動發光基因。其中及67^既含有發光酶厶基因又含有脂肪酸還原酶基因，可將脂肪酸還原成脂肪醛，直接提供發光酶於發光時所需的受質，因此無需額外填加其它如脂肪醛類之受質。其中’質體pGEM3-Zf+為promega公司之商品化的質體 (Madison，WI，USA)，有 3, 199bp。含可大量複製的 C〇lEl 複製子 '多克隆位點及對氨比西林（amp i c i 11丨η，Amp)之抗性基因’但屬窄寄主範圍的C〇iEl型複製子，且為不穩定的質體’容易於數代繁磕後遺漏掉質體於細菌中。其中’步驟（a)所構築的質體p3ZLux4有11，766bp，為可大量複製的發光質體’但本身不穩定。 (b)、構築質體 pbS-SE34 ( 1 1 ):將質體 pBlueScript II ks (+/-)以/如丨限制酶切出並接入質體pSE34中而得之質體。其中質體 pB 1 ueScr i pt 11 KS (+ /-)為 Stratagene 公司之商品化的載體（La J〇1 la , CA，USA)。含可大量複製的C〇1E1複製子、多克隆位點及對氨比西林（ampici 11 in ，Amp)之抗性基因。其中’質體pSE34(SEQ ID NO 2)有32, 950kb，來自腸内 201142028 菌的一種穩定的原生型腸炎沙門氏菌 serotype Enteritidis)，其噬菌體分型為 PT34 。腸炎沙門氏菌是世界上（包括台灣）造成動.物及人的沙門氏菌相關病症的主要病源，其中噬菌體分型PT34是台灣第二普遍的腸炎沙門氏菌。此噬菌體分型PT34經本申請人研究團隊研究發現是來自最普遍的嗤菌體分型PT4之腸炎沙門氏菌，噬菌體分型PT4之腸炎沙門氏菌因為獲得質體 PSE34後改變而成為噬菌體分型ρΤ34。本申請人研究團隊也發現此質體擁有/?//·、仰/·^1、ρπ/1、以办厶、及$广从基因 ’有助於細胞增殖分裂時，質體可穩定地分配於子細菌中。认反仓 pilXl、pi1X2、piiX4、pilX5、piixe、pilX7、 pi 1X8 ' pi 1X9、pi 1X1〇、pi 1X11、taxA、taxB 反 taxc I 私可幫助質體在細菌與其他菌種細菌間以接合生殖作用 (con jugat ion)來散佈。ID NO 16), at least one gene (SEQ ID NO 17), at least one taxA gene (SEQ ID NO 18), at least one gene (SEQ ID NO 19) and at least one iaxC gene (SEQ ID NO 20). Wherein, the promoter capable of initiating the spontaneous luminescent gene is the PlacZ promoter (SEQ ID NO 22). Wherein, the resistance gene may be a resistance gene to ampicillin (amp ici 11 i η, Amp) or a resistance gene to kanamycin (Kan), or contain ampicillin (amp ici) The resistance gene of 11 i η, Amp) plus two resistance genes to the resistance gene of kanamycin (Kan). In the sputum, the pjr, parG 'parF, stbD and stbE are related genes after stabilization; the pi///, p///heart Ρ/ΛΤ5, pi 1X8, pi 1X9, pi 1X10, pi 1X11, taxA , taxB and taxC are related to the reproductive-related genes, which contribute to the zygosity of the plastids, help the colonization of the plastids and the transfer between bacteria and other bacteria (such as the transfer of plastids from Salmonella to E. coli). . Among them, the /z/W million spontaneous luminescent gene contains both the luminescent enzyme gene and the fatty acid reductase gene, which can synthesize fatty aldehyde and directly supply the susceptor to the substrate, so no additional substrate (such as fat) is needed. 201142028 ^ 真加. The plastid pSE-Luxl of the present invention can express spontaneous luminescent gene protein, can be stably luminescence in the presence of bacteria, and can be stabilized in the breeding of bacteria. 旦令令疋疋疋疋疋疋疋疋疋疋疋疋疋疋It is more convenient for direct observation in living animals. The preparation steps of the stable spontaneous luminescent vector for the Gram-negative bacteria of the present invention are as follows: (a) A luminescent gene lacking a promoter (accessed to a plastid containing a promoter, The luminescent gene (/ and αβ) can be used to initiate transcription using a promoter. (b) A Gram-negative bacterial plastid containing at least one ColEl replicon and at least one resistance gene is inserted into the plastid pSE34. The plastid constructed in the step (a) is introduced into the plastid constructed in the step (b) to obtain a stable spontaneous luminescent support for the gram-negative bacteria. [Examples] Please refer to the first to third B-pictures. ,this The detailed preparation steps for the preparation of stable spontaneous luminescent vectors for the preparation of Gram-negative bacteria are as follows: (a) Construction of the plastid p3ZLux4 (1 〇): luminescence gene lacking the promoter from the plastid pXen-5 as a restriction enzyme The plastid PGEM3-Zf+ containing the PucZ promoter was excised, and the luminescent gene (/仏 can be activated by the PUcZ promoter in the plastid pGEM3-Zf+. Among them, the plastid pXen-5 is Xenogen The company's commercial plastid 201142028 (Alameda, CA, USA) has 18, 357 base pairs (bp). It contains a resistance gene for kanamycin (Kan) and a spontaneous luminescent gene. However, the promoter is lacking to start, so that the luminescent gene cannot be activated. Among them, 67^ contains both the luminescent enzyme gene and the fatty acid reductase gene, which can reduce the fatty acid to fatty aldehyde and directly provide the luminescent enzyme required for luminescence. Quality, so there is no need to add other substances such as fatty aldehydes. The 'plasty pGEM3-Zf+ is a commercial plastid of promega (Madison, WI, USA) with 3, 199 bp. C〇lEl replicon 'multiple cloning site and The resistance gene of ampicillin (amp ici 11丨η, Amp) is a C〇iEl-type replicon of a narrow host range, and is an unstable plastid. It is easy to miss the plastid after several generations of turbidity. Among the bacteria, the plastid p3ZLux4 constructed in step (a) has 11,766 bp, which is a luminescent body that can be reproduced in a large amount, but is unstable itself. (b) Constructing a plastid pbS-SE34 (1 1 ): The plastid pBlueScript II ks (+/-) is obtained by restriction enzyme digestion and access to the plasmid pSE34. The plastid pB 1 ueScr i pt 11 KS (+ /-) is a commercial carrier of Stratagene (La J〇1 la , CA, USA). It contains a large number of replicable C〇1E1 replicons, multiple cloning sites and resistance genes to ampicillin (ampic 11 in , Amp). Among them, the plastid pSE34 (SEQ ID NO 2) has 32, 950 kb, a stable native S. serotype Enteritidis from the intestine 201142028, and its phage type is PT34. Salmonella enteritidis is the main cause of Salmonella-related disorders in the world, including Taiwan, where phage typing PT34 is the second most common Salmonella enteritidis in Taiwan. This phage typing PT34 was found by the research team of the Applicant to be the most common Salmonella enteritidis from PT4, and the phage typing PT4 was changed to phage typing ρΤ34 after obtaining plastid PSE34. The applicant's research team also found that the plastid possesses /?//·, YANG /·^1, ρπ/1, 厶厶, and $广从基因' to help cell proliferation and division, the plastid can be stably It is distributed in the sub-bacteria. Recognition of pilXl, pi1X2, piiX4, pilX5, piixe, pilX7, pi 1X8 ' pi 1X9, pi 1X1 〇, pi 1X11, taxA, taxB anti-taxc I can help the plastids to zygote between bacteria and other bacteria Conjugation is used to spread.

其中’步驟（b)所構築的質體pBS — SE34有34_ 6kb，具有質體PSE34的優點，含有穩定質體之相關基因和接合生殖作用相關的基因特性，以及具有質體pBlueScript π B 的優點，含可大量複製的ColE1複製子、多克隆位點及對氨比西林（ampici 11 in ’ Amp)之抗性基因。 (c)、構築質體pSE_Luxl (丄2 ):利用純限制酶及乃〇I限制酶分別切割第（a)步驟所構築之質體p3ZLux4 及第⑻步驟所構築之f體娜_聰，再將質體邮㈤及 201142028 質體PBS-SE34接合成一個質體PSE-Luxl，該質體pSE-Luxl 有46. 3kb ’為一革蘭陰性細菌專用之穩定自發發光載體。〔功能測試〕請參閱下表一’為本發明之質體pSE-Luxl穩定性測試 ’我們將質體p3Zlux4植入選殖株大腸桿菌（兄co//)中當作對照組’將質體pSE-Luxl植入沙門氏菌（51· Typh imurium LBNP4417)中當作實驗組，分別在不含抗生素篩選的 Luria-Bertani(LB)液體培養基中培養79代後，此菌液於不同稀釋倍數下分別塗盤於不含抗生素及含抗生素（如 ampici 11 in及kanamycin)之LB洋菜膠（含1.5%的洋菜膠）培養皿中生長’隔夜後計算總細菌數及含質體的細菌數 (colony formation unit，CFU)，可以清楚的發現本發明之質體pSE-Luxl可以在79代後，仍有75%(1550/2030)的細菌仍具有發光質體，細菌經四天（約7 9代）之繼代培養後，發現本發明之發光質體pSE-Lux 1存在細菌内的穩定效率優於質體p3ZLux4約十萬倍，在培養皿上觀察菌落發光情形，質體p3ZLux4所產生的發光效果約8小時，而質體pSE-Luxl 發光至少2天以上。此結果顯示本發明所構築的質體 pSE-Luxl既可表現自發發光基因蛋白質，又可非常穩定的存在細菌中持續發光。 201142028 表一、質體pSE-Luxl穩定性測試 CFU/ml 植入質體p3Zlux4的大腸桿菌植入質體pSE-Lux 1的沙門氏菌 LB培養基含Amp與Kan之LB 培養液 LB培養基含Amp與Kan之LB 培養基 102 菌數過多無法計算 43 菌數過多無法計算菌數過多無法計算 103 菌數過多無法計算 4 菌數過多無法計算菌數過多無法計算 104 菌細多無法計算 0 菌數過多無法·計算菌數過多無法計算 ΙΟ5 菌鑛多無法計算 0 菌數過多無法計算菌數過多無法計算 ΙΟ6 488 0 2,030 1,550 前文係針對本發明之較佳實施例為本發明之技術特徵進行具體之說明；惟，熟悉此項技術之人士當可在不脫離本發明之精神與原則下對本發明進行變更與修改，而該等變更與修改，皆應涵蓋於如下申請專利範圍所界定之範疇中〇【圖式簡單說明】第一圖：本發明其一實施例之步驟流程圖。第二A圖：本發明其一實施例之質體pXen-5架構圖。第二B圖：本發明其一實施例構築質體p3ZLux4示意圖。第三A圖：本發明其一實施例之質體pSE34架構圖。第三B圖：本發明其一實施例構築質體pSE-Luxl示意圖。第四圖：為本發明其一實施例之質體pSE-Luxl架構圖。【主要元件符號說明】 (1 0 )構築質體p3ZLux4 (1 1 )構築質體PBS-SE34 (1 2 )構築質體pSE-Luxl 12 201142028 序列表 <110>長庚大學 <120>革蘭陰性細菌專用之穩定自發發光載體及其製作方法 <160>22 <210>SEQ ID NO 1' <211>46,313 <212>DNA <213>人工序列 <220> <223>革蘭陰性細菌之穩定可自發發光質體 <400>1 tgtcactgat acatggcggt aaactatatc tgatgccctg gactgccatt attgacaggc agcagggcag caggccgaaa taagaaaatc taagggattt gaaaacaggg ctttttcagt cgttacaagc gcagaactgg cattaaacaa agccggacta cagttgctcg gaacgtgaag atcgccggag tcacgtaaaa acgaaatact gggcgttctc taatatggca atacatccta ccggtgtggc gtgaggtagt ctccccctgt gggcgaagag cggtggagct ttacaacgtc ccccctttcg ttgcgcagcc taaatttttg ataaatcaaa cactattaaa gcccactacg taaatcggaa tggcgagaaa cggtcacgct caggtggcac attcaaatat aaaggaagag ccaacgatta attggtacgc acgctggaaa ccaggtatag gaaaatggcg atgatttctg tttcaacgcc ggttatttct acccaggcac aaaaagaaaa aagctgggtt taagaaaatg tgcctttatc tgatgaataa gaggattaat tgatgaatat aaatgcagga cggctgaacg agggtttctc aaacgttacc ggtctggtcg tggaagattt aggatatagt caaacaaatt aggaaagtca tggtctggtg ccctggtgat acgctaaact ccaattcgcc gtgactggga ccagctggcg tgaatggcga ttaaatcagc agaatagacc gaacgtggac tgaaccatca ccctaaaggg ggaagggaag gcgcgtaacc ttttcgggga gtatccgctc tatgagtatt aaaaattact cagctacccg aagggaaact cggttaatcc acctgacggt atgttgacct tggactctcc gtaccggatg aggccgaaaa gtggtggaac ttccggcaag tacatcaaga agaatcagag actttatgat tatgagcgaa tcgttcattg aaagctcaac agcagagcgt accggaagaa aaaagcgcca tggacgtgcg tcgtatcaat tttatatcat atccctgcga acagtatacc acggttcgtg tatttgcata atattcatat ctatagtgag aaaccctggc taatagcgaa atggaaattg tcatttttta gagatagggt tccaacgtca ccctaatcaa agcccccgat aaagcgaaag accacacccg aatgtgcgcg atgagacaat caacatttcc gaaagataag tgcagccatt tattccgact tgatatgaca tgaacagttt gggcaagatg ctgcccttcc tgaatttaaa actggttaaa gtatgataca ggctaagaag gatactcttg gcattttatt aaggttcctt ctgactaaag cgtgtgtttg gttgttattg gaacggaaac ctgcttggtc cccaagtatc ccaaaaccga aagagtttga cattttgtta cagactggta aactggctgc atgataatgg aagaacaact aaagcctctg tcgtattacg gttacccaac gaggcccgca taagcgttaa accaataggc tgagtgttgt aagggcgaaa gttttttggg ttagagcttg gagcgggcgc ccgcgcttaa gaacccctat aaccctgata gtgtcgccct gataaagaca ctggaaacgc gataccggat gcattatggt attaatgagc aagattgaac tgtggtaaac atctggagtg tcagggaaaa gttcttgtcc tgaaaacaaa aaaaagttat ctgccgcaga ccggcgtatg aagatgaata cccgtgagat aagaacgtcg gtcaggaact tgtccgaaga aatttgaaga ttgatgaagc acggagtaac aggaagaaaa tttccgtcac atgggcgctt gcgtcctaaa caccgatgat ttctagagcg cgcgctcact ttaatcgcct ccgatcgccc tattttgtta cgaaatcggc tccagtttgg aaccgtctat gtcgaggtgc acggggaaag tagggcgctg tgcgccgcta ttgtttattt aatgcttcaa tattcccttt aaaaagatga tgaagaagag atgcgcttat ctgaaaagca tgtacggtga ccgctgcgcc atattgttat agttttctgg ccgatttgat ttgaggataa gcaggaatgg tgagattaac tcaccgccgt gaagtacgtc cggcattatc tgattttgag tgaagatgct gcttcagtta agcaccaaaa aaatggtgaa gttgaaagcc agatgagcag tccatgagta gataatgtct aaagaaaatg ctggttactc gaaaaagagt gccgccaccg ggccgtcgtt tgcagcacat ttcccaacag aaattcgcgt aaaatccctt aacaagagtc cagggcgatg cgtaaagcac ccggcgaacg gcaagtgtag cagggcgcgt ttctaaatac taatattgaa tttgcggcat oooooooooooooooooooooooooooooooooooo 628406284062840628406284062840628406 11233445667789900122334556678899011223445 -111222222222 1X 11 1x 11 11 ΙΑ -1 ΙΑ IX 11 IX 1± ΌΌ208040006020Among them, the plastid pBS-SE34 constructed in step (b) has 34-6 kb, which has the advantages of plastid PSE34, contains genes related to stable plastids and gene characteristics related to zygosity, and has the advantage of plastid pBlueScript π B . It contains a large number of copies of the ColE1 replicon, a multiple cloning site, and a resistance gene to ampicillin (ampici 11 in 'Amp). (c) Construction of the plastid pSE_Luxl (丄2): The plastid p3ZLux4 constructed in the (a) step and the f-body Na-Cong constructed in the (8) step are respectively cut by the pure restriction enzyme and the 〇I restriction enzyme, and then The plastid (5) and 201142028 plastid PBS-SE34 were ligated into a plastid PSE-Luxl, which has 46.3 kb' as a stable spontaneous luminescent vector for gram-negative bacteria. [Functional test] Please refer to the following table 1. 'The plastid pSE-Luxl stability test of the present invention'. We used the plastid p3Zlux4 implanted in the Escherichia coli (brother co//) as a control group. pSE-Luxl was implanted into Salmonella (51. Typh imurium LBNP4417) as an experimental group, and after being cultured for 79 generations in Luria-Bertani (LB) liquid medium without antibiotic screening, the bacterial solution was separately coated at different dilutions. The plate is grown in a Petri dish containing no antibiotics and antibiotics (such as ampici 11 in and kanamycin) in a Petri dish (containing 1.5% of acacia). Calculate the total number of bacteria and the number of bacteria containing plastids after overnight (colony Formation unit, CFU), it can be clearly found that the plastid pSE-Luxl of the present invention can still have 75% (1550/2030) of bacteria still having luminescent plastids after 79 generations, and the bacteria pass through four days (about 7 9 generations). After subculture, it was found that the luminescent plastid pSE-Lux 1 of the present invention has a stability efficiency in bacteria higher than that of the plastid p3ZLux4, and the luminescence of the plastid p3ZLux4 is observed on the culture dish. The effect is about 8 hours, while the plastid pSE-Luxl glows at least 2 More than a day. This result shows that the plastid pSE-Lux1 constructed by the present invention can express both spontaneous luminescent gene proteins and persistent luminescence in bacteria which are very stable. 201142028 Table I, plastid pSE-Luxl stability test CFU/ml E. coli implanted with plastid p3Zlux4 Implanted into plastid pSE-Lux 1 Salmonella LB medium containing Amp and Kan LB medium LB medium containing Amp and Kan LB medium 102 too many bacteria can not be counted 43 too many bacteria can not calculate the number of bacteria too much can not be calculated 103 too many bacteria can not be calculated 4 too many bacteria can not count too many bacteria can not be calculated 104 bacteria can not calculate more than 0 bacteria can not count · calculation bacteria Too many can not be calculated ΙΟ5 bacteria can not be counted 0 too many bacteria can not calculate too many bacteria can not be calculated ΙΟ 6 488 0 2,030 1,550 The foregoing is a specific description of the technical features of the present invention for the preferred embodiment of the present invention; The present invention may be modified and modified by those skilled in the art without departing from the spirit and scope of the invention, and such changes and modifications are to be included in the scope defined by the following claims. First Figure: Flow chart of the steps of an embodiment of the present invention. Figure 2A is a diagram of a plastid pXen-5 architecture of an embodiment of the present invention. Second B: A schematic diagram of the plastid p3ZLux4 is constructed in accordance with an embodiment of the present invention. Figure 3A is a diagram of a plastid pSE34 architecture of an embodiment of the present invention. Third B: A schematic diagram of the plastid pSE-Luxl is constructed in accordance with an embodiment of the present invention. Fourth: A plastid pSE-Luxl architecture diagram of an embodiment of the present invention. [Major component symbol description] (1 0) Construct plastid p3ZLux4 (1 1 ) Construct plastid PBS-SE34 (1 2 ) Construct plastid pSE-Luxl 12 201142028 Sequence table <110> Chang Gung University <120> Gram Stable spontaneous luminescent vector for negative bacteria and method for producing the same <160>22 <210> SEQ ID NO 1 ' <211>46,313 <212>DNA<213> artificial sequence<220><223> Gram stable negative bacteria of a spontaneously light emitting plasmid < 400 > 1 tgtcactgat acatggcggt aaactatatc tgatgccctg gactgccatt attgacaggc agcagggcag caggccgaaa taagaaaatc taagggattt gaaaacaggg ctttttcagt cgttacaagc gcagaactgg cattaaacaa agccggacta cagttgctcg gaacgtgaag atcgccggag tcacgtaaaa acgaaatact gggcgttctc taatatggca atacatccta ccggtgtggc gtgaggtagt ctccccctgt gggcgaagag cggtggagct ttacaacgtc ccccctttcg ttgcgcagcc taaatttttg ataaatcaaa Cactattaaa gcccactacg taaatcggaa tggcgagaaa cggtcacgct caggtggcac attcaaatat aaaggaagag ccaacgatta attggtacgc acgctggaaa ccaggtatag gaaaatggcg atgatttctg tttcaacgcc ggttatttct acccaggcac aaaaagaaaa aagctgggt t taagaaaatg tgcctttatc tgatgaataa gaggattaat tgatgaatat aaatgcagga cggctgaacg agggtttctc aaacgttacc ggtctggtcg tggaagattt aggatatagt caaacaaatt aggaaagtca tggtctggtg ccctggtgat acgctaaact ccaattcgcc gtgactggga ccagctggcg tgaatggcga ttaaatcagc agaatagacc gaacgtggac tgaaccatca tatgagtatt ccctaaaggg ggaagggaag gcgcgtaacc ttttcgggga gtatccgctc actttatgat tatgagcgaa tcgttcattg aaaaattact cagctacccg aagggaaact cggttaatcc acctgacggt atgttgacct tggactctcc gtaccggatg aggccgaaaa gtggtggaac ttccggcaag tacatcaaga agaatcagag aaagctcaac agcagagcgt accggaagaa aaaagcgcca tggacgtgcg tcgtatcaat tttatatcat atccctgcga acagtatacc acggttcgtg tatttgcata atattcatat ctatagtgag aaaccctggc taatagcgaa atggaaattg tcatttttta gagatagggt tccaacgtca ccctaatcaa agcccccgat aaagcgaaag accacacccg aatgtgcgcg atgagacaat caacatttcc gaaagataag tgcagccatt tattccgact tgatatgaca tgaacagttt gggcaagatg ctgcccttcc tgaatttaaa actggttaaa gtatgataca ggctaagaag gatactcttg gcattttatt aaggttcctt ctgactaaag cgtgtgtttg gttgttattg gaacggaa ac ctgcttggtc cccaagtatc ccaaaaccga aagagtttga cattttgtta cagactggta aactggctgc atgataatgg aagaacaact aaagcctctg tcgtattacg gttacccaac gaggcccgca taagcgttaa accaataggc tgagtgttgt aagggcgaaa gttttttggg ttagagcttg gagcgggcgc ccgcgcttaa gaacccctat aaccctgata gtgtcgccct gataaagaca ctggaaacgc gataccggat gcattatggt attaatgagc aagattgaac tgtggtaaac atctggagtg tcagggaaaa gttcttgtcc tgaaaacaaa aaaaagttat ctgccgcaga ccggcgtatg aagatgaata cccgtgagat aagaacgtcg gtcaggaact tgtccgaaga aatttgaaga ttgatgaagc acggagtaac aggaagaaaa tttccgtcac atgggcgctt gcgtcctaaa caccgatgat ttctagagcg cgcgctcact ttaatcgcct ccgatcgccc tattttgtta cgaaatcggc tccagtttgg aaccgtctat gtcgaggtgc acggggaaag tagggcgctg tgcgccgcta ttgtttattt aatgcttcaa tattcccttt aaaaagatga tgaagaagag atgcgcttat ctgaaaagca tgtacggtga ccgctgcgcc atattgttat agttttctgg ccgatttgat ttgaggataa gcaggaatgg tgagattaac tcaccgccgt gaagtacgtc cggcattatc tgattttgag tgaagatgct gcttcagtta agcaccaaaa aaatggtgaa gttgaaagcc agatgagcag tccatgagta gataatgtct aaagaaa atg ctggttactc gaaaaagagt gccgccaccg ggccgtcgtt tgcagcacat ttcccaacag aaattcgcgt aaaatccctt aacaagagtc cagggcgatg cgtaaagcac ccggcgaacg gcaagtgtag cagggcgcgt ttctaaatac taatattgaa tttgcggcat oooooooooooooooooooooooooooooooooooo 628406284062840628406284062840628406 11233445667789900122334556678899011223445 -111222222222 1X 11 1x 11 11 ΙΑ -1 ΙΑ IX 11 IX 1 ± ΌΌ208040006020

201142028 tttgccttcc agttgggtgc gttttcgccc cggtattatc agaatgactt taagagaatt tgacaacgat taactcgcct acaccacgat ttactctagc cacttctgcg agcgtgggtc tagttatcta agataggtgc tttagattga ataatctcat tagaaaagat aaacaaaaaa tttttccgaa agccgtagtt taatcctgtt caagacgata agcccagctt aaagcgccac gaacaggaga tcgggtttcg gcctatggaa ttgctcacat ttgagtgagc aggaagcgga aatgcagctg atgtgagtta tgttgtgtgg acgccaagcg tcgacaagct tctgtattct attacttgaa aactatatat ttaatctatt aaaaatttta atgcgcttgt gaagtcatta tcaacttaat taatcaaaga attgtaattt ggtattacag gagataaagt acaatgtttt tacactgaaa taaaaatatt tcaagcaaaa gcgcagcttt ccatttttca aatgttggaa actttgtggt tatggttaac cgatgtactc agcgattgga cgaaagcgaa tacggaactc tgtttttgct acgagtgggt cgaagaacgt ccgtattgac ggttgagtac atgcagtgct cggaggaccg tgatcgttgg gcctgtagca ttcccggcaa ctcggccctt tcgcggtatc cacgacgggg ctcactgatt tttaaaactt gaccaaaatc caaaggatct accaccgcta ggtaactggc aggccaccac accagtggct gttaccggat ggagcgaacg gcttcccgaa gcgcacgagg ccacctctga aaacgccagc gttctttcct tgataccgct agagcgccca gcacgacagg gctcactcat aattgtgagc cgcaattaac caagggattc cccaatgcta cagggtaaat agaataaaaa taacatctag tcagattcaa gacttattag gtagataatg catgctattt tgtgtatgta ttcttttaac ctttaattgg ccgtataatt taacgacaaa agcgaagaga ttaacaacgg gaatatgaac acgacacgtg cccacagtgt atgtatgtat aaatccgtct gagtggcaga tatataaaag ataaccaaag gagacctgga gttatttctg cacccagaaa tacatcgaac tttccaatga gccgggcaag tcaccagtca gccataacca aaggagctaa gaaccggagc atggcaacaa caattaatag ccggctggct attgcagcac agtcaggcaa aagcattggt catttttaat ccttaacgtg tcttgagatc ccagcggtgg ttcagcagag ttcaagaact gctgccagtg aaggcgcagc acctacaccg gggagaaagg gagcttccag cttgagcgtc aacgcggcct gcgttatccc cgccgcagcc atacgcaaac tttcccgact taggcacccc ggataacaat cctcactaaa aagtcgccaa aagacccaca ctataagtac atagtaaata gaggtttatt gtaggttaga aacactttca aattagttac tagatgatat aaaatgtgaa tttaaccttt agctgttgtt gtgtaaaagt aacataccca ttcaaagcat tatttaatca gaacagaaaa taggcacgct ttgaacgtta caggcgtttc ctaagtcctt atcgtttatt tacgagaaga atggcgaccg caacattttt atgcgcacaa cgctggtgaa tggatctcaa tgagcacttt agcaactcgg cagaaaagca tgagtgataa ccgctttttt tgaatgaagc cgttgcgcaa actggatgga ggtttattgc tggggccaga ctatggatga aactgtcaga ttaaaaggat agttttcgtt ctttttttct tttgtttgcc cgcagatacc ctgtagcacc gcgataagtc ggtcgggctg aactgagata cggacaggta ggggaaacgc gatttttgtg ttttacggtt ctgattctgt gaacgaccga cgcctctccc ggaaagcggg aggctttaca ttcacacagg gggaacaaaa agaaaaaggt aaagcgttta tatagctaaa aaataacata catgtcttat aatattagat attctcacaa aacacgaaaa tatccaaaac atcaggtgac gtgatttggc gctatcatta aaaaaggcca ggaggacttt tattgaatat actaatggaa ccgacaaagt agaattaaaa tcaacgaaac aactcgtaaa cgtttctagc atcagaaaaa aaatcgagta tgaaattatc tgaataccta aggattagtc agtaaaagat cagcggtaag taaagttctg tcgccgcata tcttacggat cactgcggcc gcacaacatg cataccaaac actattaact ggcggataaa tgataaatct tggtaagccc acgaaataga ccaagtttac ctaggtgaag ccactgagcg gcgcgtaatc ggatcaagag aaatactgtc gcctacatac gtgtcttacc aacggggggt cctacagcgt tccggtaagc ctggtatctt atgctcgtca cctggccttt ggataaccgt gcgcagcgag cgcgcgttgg cagtgagcgc ctttatgctt aaacagctat gctgggtacc atatataaag gtttattacc gaagttggta gacaataatc aaagaactat ttactttctt cctacactaa gacggcaata ttgaacatca tgttgatgac aaccaaaagg caggagtcgt tataacagtc tacatgaccc tctgtaaagg aatcaacgaa caacgaaatg gtacccagaa gaaaaagccc gtatcaaaaa ttaacagaac aattatcctt ctctcaaaaa ggcttcatga aaagaacgcg tctgccatta gctgaagatc atccttgaga ctatgtggcg cactattctc ggcatgacag aacttacttc ggggatcatg gacgagcgtg ggcgaactac gttgcaggac ggagccggtg tcccgtatcg cagatcgctg tcatatatac atcctttttg tcagaccccg tgctgcttgc ctaccaactc cttctagtgt ctcgctctgc gggttggact tcgtgcacac gagctatgag ggcagggtcg tatagtcctg ggggggcgga tgctggcctt attaccgcct tcagtgagcg ccgattcatt aacgcaatta ccggctcgta gaccatgatt gggccccccc ggagacctgt gagttgttga ttacacgtca tatatagatg caacaatatt gtggtgagct gtcatcatat aacattggta ttaatacatc tattttagca tttagatatt aagtctttct cttttacggt aagtacattt atgacgtttc cagaatatat gctattatga cacgtgatgg tcatggcttc ttgtggaaga agctagaacc acttaatgac gctgtcatat ttcaaagtgg gtttacaagg gaaaatcctt 2580 2640 2700 2760 2820 2880 2940 3000 3060 3120 3180 3240 3300 3360 3420 3480 3540 3600 3660 3720 3780 3840 3900 3960 4020 4080 4140 4200 4260 4320 4380 4440 4500 4560 4620 4680 4740 4800 4860 4920 4980 5040 5100 5160 5220 5280 5340 5400 5460 5520 5580 5640 5700 5760 5820 5880 5940 6000 6060 6120201142028 tttgccttcc agttgggtgc gttttcgccc cggtattatc agaatgactt taagagaatt tgacaacgat taactcgcct acaccacgat ttactctagc cacttctgcg agcgtgggtc tagttatcta agataggtgc tttagattga ataatctcat tagaaaagat aaacaaaaaa tttttccgaa agccgtagtt taatcctgtt caagacgata agcccagctt aaagcgccac gaacaggaga tcgggtttcg gcctatggaa ttgctcacat ttgagtgagc aggaagcgga aatgcagctg atgtgagtta tgttgtgtgg acgccaagcg tcgacaagct tctgtattct attacttgaa aactatatat ttaatctatt aaaaatttta atgcgcttgt gaagtcatta tcaacttaat taatcaaaga attgtaattt ggtattacag gagataaagt acaatgtttt tacactgaaa taaaaatatt tcaagcaaaa gcgcagcttt ccatttttca aatgttggaa actttgtggt tatggttaac cgatgtactc agcgattgga cgaaagcgaa tacggaactc tgtttttgct acgagtgggt cgaagaacgt ccgtattgac ggttgagtac atgcagtgct cggaggaccg tgatcgttgg gcctgtagca ttcccggcaa ctcggccctt tcgcggtatc cacgacgggg ctcactgatt tttaaaactt gaccaaaatc caaaggatct accaccgcta ggtaactggc aggccaccac accagtggct gttaccggat ggagcgaacg gcttcccgaa gcgcacgagg ccacctctga aaacgccagc gttctttcct tgataccgct agagcgccca gcacgacagg gctcactcat aattgtgagc cgcaattaac caagggattc cccaatgcta cagggtaaat agaataaaaa taacatctag tcagattcaa gacttattag gtagataatg catgctattt tgtgtatgta ttcttttaac ctttaattgg ccgtataatt taacgacaaa agcgaagaga ttaacaacgg gaatatgaac acgacacgtg cccacagtgt atgtatgtat aaatccgtct gagtggcaga tatataaaag ataaccaaag gagacctgga gttatttctg cacccagaaa tacatcgaac tttccaatga gccgggcaag tcaccagtca gccataacca aaggagctaa gaaccggagc atggcaacaa caattaatag ccggctggct attgcagcac agtcaggcaa aagcattggt catttttaat ccttaacgtg tcttgagatc ccagcggtgg ttcagcagag ttcaagaact gctgccagtg aaggcgcagc acctacaccg gggagaaagg gagcttccag cttgagcgtc aacgcggcct gcgttatccc cgccgcagcc atacgcaaac tttcccgact taggcacccc cctcactaaa ggataacaat tacgagaaga atggcgaccg caacattttt aagtcgccaa aagacccaca ctataagtac atagtaaata gaggtttatt gtaggttaga aacactttca aattagttac tagatgatat aaaatgtgaa tttaaccttt agctgttgtt gtgtaaaagt aacataccca ttcaaagcat tatttaatca gaacagaaaa taggcacgct ttgaacgtta caggcgtttc ctaagtcctt atcgtttatt atgcgcacaa cgctggtgaa tggatctcaa tgagcacttt agcaactcgg cagaaaagca tgagtgataa ccgctttttt tgaatgaagc cgttgcgcaa actggatgga ggtttattgc tggggccaga ctatggatga aactgtcaga ttaaaaggat agttttcgtt ctttttttct cgcagatacc ctgtagcacc gcgataagtc ggtcgggctg aactgagata cggacaggta ggggaaacgc gatttttgtg ttttacggtt ctgattctgt gaacgaccga cgcctctccc ggaaagcggg aggctttaca ttcacacagg gggaacaaaa agaaaaaggt aaagcgttta tatagctaaa aaataacata catgtcttat aatattagat attctcacaa aacacgaaaa tatccaaaac atcaggtgac gtgatttggc gctatcatta aaaaaggcca ggaggacttt tattgaatat actaatggaa ccgacaaagt tttgtttgcc agaattaaaa tcaacgaaac aactcgtaaa cgtttctagc atcagaaaaa aaatcgagta tgaaattatc tgaataccta aggattagtc agtaaaagat cagcggtaag taaagttctg tcgccgcata tcttacggat cactgcggcc gcacaacatg cataccaaac actattaact ggcggataaa tgataaatct tggtaagccc acgaaataga ccaagtttac ctaggtgaag ccactgagcg gcgcgtaatc ggatcaagag aaatactgtc gcctacatac gtgtcttacc aacggggggt cctacagcgt tccggtaagc ctggtatctt atgctcgtca cctggccttt ggataaccgt gcgcagcgag cgcgcgttgg cagtgagcgc ctttatgct t aaacagctat gctgggtacc atatataaag gtttattacc gaagttggta gacaataatc aaagaactat ttactttctt cctacactaa gacggcaata ttgaacatca tgttgatgac aaccaaaagg caggagtcgt tataacagtc tacatgaccc tctgtaaagg aatcaacgaa caacgaaatg gtacccagaa gaaaaagccc gtatcaaaaa ttaacagaac aattatcctt ctctcaaaaa ggcttcatga aaagaacgcg tctgccatta gctgaagatc atccttgaga ctatgtggcg cactattctc ggcatgacag aacttacttc ggggatcatg gacgagcgtg ggcgaactac gttgcaggac ggagccggtg tcccgtatcg cagatcgctg tcatatatac atcctttttg tcagaccccg tgctgcttgc ctaccaactc cttctagtgt ctcgctctgc gggttggact tcgtgcacac gagctatgag ggcagggtcg tatagtcctg ggggggcgga tgctggcctt attaccgcct tcagtgagcg ccgattcatt aacgcaatta ccggctcgta gaccatgatt gggccccccc ggagacctgt gagttgttga ttacacgtca tatatagatg caacaatatt gtggtgagct gtcatcatat aacattggta ttaatacatc tattttagca tttagatatt aagtctttct cttttacggt aagtacattt atgacgtttc cagaatatat gctattatga cacgtgatgg tcatggcttc ttgtggaaga agctagaacc acttaatgac gctgtcatat ttcaaagtgg gtttacaagg gaaaatcctt 2580 2640 2700 2760 2820 2880 2940 3000 3060 3120 3180 3240 3300 3360 3420 3480 3540 3600 3660 3720 3780 3840 3900 3960 4020 4080 4140 4200 4260 4320 4380 4440 4500 4560 4620 4680 4740 4800 4860 4920 4980 5040 5100 5160 5220 5280 5340 5400 5460 5520 5580 5640 5700 5760 5820 5880 5940 6000 6060 6120

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201142028 caccaacgta agttggcaaa gatgccaagt tcacttccta agaaatatct ttaccaccat tcctaaaaaa aattcaaaat ctttcagaga agctgttaaa ggaattttta agttcacaga tattaactta gcgcgtgagg ctaaaaatcg attgattcat gattatatcg atcaaccaaa atattcaaaa gcttgcgcat cattggatga tggattcgaa gacgcctttc aatataccgt acaaggaaat tcccacaatc gactaaagag taccaatcta attgaacgac tgaatcaaga agtacgcaga agagaaaaga ttattcgcat cttccccaat caaacatcag ccaatcgctt aattggagcc gttcttatgg acctacatga tgaatggatt tattcttcaa gaaaatacat caattttgat aagtagaaat ggtaaaaaca ttggattcga cagttgcgga tgtacttcag aaaagattag atgtctaaaa agcttgtagt taaagctttt tagacatcta aatctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt atttccctcc tcgacttaac tatcaaacgc ttcggttaag cttaaagcac accctttctg cgtcctcgta ttgacgcgac gtaaaatttc aacgagcacg ccgggatact taccatattc tctgctaatt atcccgacat catcggtaac aataaatgct ggataactgg ttgctgacgc atccatataa ctcatcaacc ccggcgttcc atcaggtaca ggtttcaacg tttcaggatc aagcgctcgc gcatataccc acggcggaac atgtttacgc tgcatttcat cctcaaagaa acaagtgttg agttcaactt gattaaatat atctcggatc tgactaatat cactgagatt gaaagtatca aataaaagat gattgaaatc atcacgtttc agagattctt tttcgtaact tttccagccg cctccggtta tgatataaag gcttttatct ccagaaaatg agattttttt atctttcata taatggcaga gtaaataaat aaagtatggc gaaccaataa gacaaagatc tttcccttga ttttttattc gttcaagact attcaatgtt ttaacaaaat ctattcgttc ttctgttacg gtaaatgtcg taggatataa caattccacc aaactcataa catatttaaa ccaaatatta tgagcattaa atctatctgg tcccaaattg actaattcta tttgatgatc aaaccaacta ccaacatatt tcatgccata actcacagag cctaagagtc tctcaatact taatctgtca cgcgccacct gactttttaa accattcgtg ccgctactgg taaaccaact ttcaatctcg ttttcctgag aagttaataa gcgagtaaac ttaaaaaccg atgttgggaa tacaggtatg tcatcaattt ccgtaatatt gtcatctact ttgtgtgcct gacagtagtg acgatattct cgacaatgtt tataatgatt acgaaatgca tcaagcacaa gtttctttct gattttttcc tgctcgtcgt aagaccacac taatggatcg ctcgaaaaaa tcaaatcatc aatttctgag cttgctgtaa tttcttgttt atcaacatat gaagtcatac ctgttttcct cctcgactta agacagagaa attgcttgat tttcaatctc aattctcatt cggcgttcat tgactgtcgc aatagttaaa tgttcaaatg acggttcagt aatatcaaca tcaatatcca gatgatcatt atccatcgcg atagcggctt tcgtaaccga ttgataaaaa ttgcgcagga ccactaaatt ttcactcaag tcatgcgaac ttcctaacaa agaatatatc ttgcatcgat tactacgaat atttgataac aatgtgataa cttcatcttg cttgacccaa ttatcgttat ttgcagtaaa agcaataaac ggtatatcaa gatacatcat gttattaatt gtagaagcta aatcttccca accaaaatca agacaatctc tcgcaaagac ttcagcaccc aatttatggc cttcaaaatc tagattatcc ggcaattcat taatgggtag actgagataa tcaaacccta aagctctttc aagagaatat cttaagttaa caacaccgac tgcggtgatt aaaaacgaag cattgatttc agataggctt gcataagcta tccgcgcaga taagcttgaa gccaacatac cgaagttatt tatttttcgt gtagttaacc aatcaaccac tgctaacaag ctctgctttc ctatagacat tgtaaattca tcaattgtcc ctgaactcaa tccaacgtgg tgaagcgaat catagcggat cacatgaaat ccattccgcg ataaatattc cgccagacca gcaaaatgat ccatcctgcg ggcaaaacca gacgcaataa taatggcatt ctttctcttt gggctgtttt cttctggcag cgtttcccaa acatgaattt ttttatttcc ttcaacacaa ataacgtggt cgatggtttt atattttgat tcattttcca tacttttact cctcctcgac ttatgggaca aatacaagga acttatcttc ttccaggaat cgagtctgtt ctatttcaac cgcaacatcc ttagccgtat agttagatgg cctttcatga gaaatatatg tcactaatcg ttgcaacggt ctcattccgt catgagatcc accaactcga aatatgttat 6180 6240 6300 6360 6420 6480 6540 6600 6660 6720 6780 6840 6900 6960 7020 7080 7140 7200 7260 7320 7380 7440 7500 7560 7620 7680 7740 7800 7860 7920 7980 8040 8100 8160 8220 8280 8340 8400 8460 8520 8580 8640 8700 8760 8820 8880 8940 9000 9060 9120 9180 9240 9300 9360 9420 9480 9540 9600 9660 9720 3 201142028 tcattcctgc ttctacaatc ctttccgcac cttttaatgc taacgcatct cgatatttaa 9780 atgatgactc ccaaggaaaa atagatatgg tttgcgtctt atttttttga acataaggca 9840 atatttgctc aatattatcg acgtgatgaa ggtacacaca tctgccaagt ggttgattaa 9900 attccacacc tgcatttgac tcaataatca tccaacgttg atgaatatcc acctctactt 9960 ttaatccagc aaacaagctt tctttttgaa ctaaagaata ggccgccttt tcatcaaaat 10020 cttttttggc attcggtaat atatgcgcat atagattaag tttttctatc aacgctaact 10080 taaattcctc ataatgattt cccatgtaat atatgttttg ggcagaaaaa caagctcgct 10140 gatcgtaaaa acaaacatca tgagccgcac ctgtcgctgc ggacgtcaaa tcaacaggat 10200 tatcgataat gcaaagactc tttttagaac caaatttaat cacatcagca taagatggcg 10260 catgctctac cgcccaatta atcgcatctg gccctcccca agcgacaata acatccgcat 10320 gtcgcataat ttcttttgcg agtgatgtat caccttggtg gggccaatat ataacagata 10380 aagagcgcgt tatcggatga ttagggtcta catcaataaa acttaacgct aatgcattag 10440 cggtaaaagg atcggttgac gatgttttta taatacactg attcttagtt aaaattgcgc 10500 gtaatataga catgatccca gataatggaa cattacctgc caacagatgt acagatttac 10560 ctttcggaaa agcccgaaca taactttcat cctgaggtag ccattcatcc atgatatggc 10620 gagaaccaag ttcattttct acaacatcat aaaggccgcc tttagaacat aaaatcatag 10680 atatccaatt ggcctctagc ttagccattt cttctgaata tcccatatat ttttttaagt 10740 cacgaatgta tgtcctgcgt cttgagtatt cttcattttt ccatctttgc cctaccgtat 10800 agagaaaatt gacaatgtta tgcaaccgta attcgttatt tccattacaa tcaataatgt 10860 tttttacatg agagtcattc aatattggca ggtaaacact attatcacca aaattaatgg 10920 attgcactaa atcatcactt tcgggaaaga tttcaacctg gccgttaata atgaatgaaa 10980 tttttttagt catatttgcc atcctcctcg acttaggtat attccatgtg gtacttctta 11040 atattatcat caacaatatt gattacattt ttttggctca tcaaatcatt cattggttca 11100 aaggacagca atacactttt cgcaccacac ttttcaattg ccaacttagc cgcagttata 11160 cactccgtat aatttccgac agcgttttct gcaattattt cttcaagttt attttcgaaa 11220 ttttcattag ggtgcatttc aagaacataa tcactaataa atgcacgcgt ctcttgttta 11280 gctttattac tatcttcgtt atagttaact aatatcatta actgatggtc tatctctgat 11340 aggtcaacgt catatttatc cgcaacggct ttatatcttt cagcatattc atatctaaca 11400 tcattagaat catcccactt aaagatgaga ggaatacctt ttttggccgc ccactcaaca 11460 atatgatgac tggttgctgt tacatatttc cgaggtccgc ctggcgtata agcatgggga 11520 tttacagata ttttagggaa gctataaaaa tcgttatctg gattacaata gcctgttgtt 11580 aaagcatcgt taatgatttc ataacactct tcaaatagtt gctgttgata ttcaaccggg 11640 cgattaaaaa aatgcatttc atcttttttt tcgcaatcac taaaccctaa aataaatctc 11700 ccttcactta actgatccaa taagcaagct tcctccgcta tggcgacagg atgatgagtt 11760 gtaatgatgt gatttaatga accaatttta attttctctg ttaaaccgag cagaaaacca 11820 gaaacagtca gaggagcgcc gacaacacca ttatctgaaa aatgattttc atacactaaa 11880 atctgttcaa aattcaactt atcaacatac tccgttattt cctgcatgcg aactatactt 11940 tgttcttgaa cagttgttga attgatgaag ttaaggaaga acaatccaaa tttcatttct 12000 ttctcctcct cgacttaata taatagcgaa cgttgttttt ctttaagaaa tggcatgaca 12060 tcagactgga agagcttcat ggaagcaata atttcgtcta ctgttccatt agcttcaaat 12120 ccacaacaaa tatttgatat tcctgtagca tcaatgtctt tttgaattat gtcaatacat 12180 tcctgcggcg ttcccacggg attgatttcg taactgtaat caatacggcg attagtatct 12240 ttatgtcctt ttaatacaaa gtcacgccac tgccctttat tgaaatcata acctcttgtt 12300 tggtctgaat catcaaaaat agtcgtagca ttcacataag aatcatacca atgccccaga 12360 aatttccggc aaatctcttt cgctttaatt gagtcatgat ctacagatgt tatatatgat 12420 aagcaatggt cgatattatg aatatcgtgc ccatattctt gagccacttc attataaagc 12480 tcaagttgtg ctttcttttc gttagtattt ataatccaac ttaatatcat cggtaggcca 12540 aattgagcag cccactcagt cgtcgaagct gattcagcca ccacataaac cggtgcgcca 12600 cctctgctat acgccgcggg gtttactttt accttatgga acttgatatg ttcattatca 12660 gcttccatat atccctctgt catgccattc tttatcagcc cgtaccagca ttccgctaag 12720 gcgcgactgt tattcatatc tgtgccgaat acgcgaaagt ccttgttgta aagccctcgg 12780 caaataccaa accgaaatcg tccttttgac atttgatcca ataaattcac atcttcaagt 12840 tggcgtactg gatgggctgt gggaagaaca atagcggcag ttcctacatt caatttttta 12900 gtcgcgccaa gtaaatatgc agcagcgaca taagggttac caagcaaacc aaactccgtg 12960 aaatgatgct ccagtaacca tacggtatca aaaccacact cctcagagat gcgacctaat 13020 ttaaccaaac gtttcattac ctctgtttga gaaaattggg gaggttggta tgtaagcaaa 13080 aagtttccaa atttcataga gagtcctcct cttgcttcat ctgcaggcat gcaagcttga 13140 gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt 13200 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 13260 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 13320201142028 caccaacgta agttggcaaa gatgccaagt tcacttccta agaaatatct ttaccaccat tcctaaaaaa aattcaaaat ctttcagaga agctgttaaa ggaattttta agttcacaga tattaactta gcgcgtgagg ctaaaaatcg attgattcat gattatatcg atcaaccaaa atattcaaaa gcttgcgcat cattggatga tggattcgaa gacgcctttc aatataccgt acaaggaaat tcccacaatc gactaaagag taccaatcta attgaacgac tgaatcaaga agtacgcaga agagaaaaga ttattcgcat cttccccaat caaacatcag ccaatcgctt aattggagcc gttcttatgg acctacatga tgaatggatt tattcttcaa gaaaatacat caattttgat aagtagaaat ggtaaaaaca ttggattcga cagttgcgga tgtacttcag aaaagattag atgtctaaaa agcttgtagt taaagctttt tagacatcta aatctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt atttccctcc tcgacttaac tatcaaacgc ttcggttaag cttaaagcac accctttctg cgtcctcgta ttgacgcgac gtaaaatttc aacgagcacg ccgggatact taccatattc tctgctaatt atcccgacat catcggtaac aataaatgct ggataactgg ttgctgacgc atccatataa ctcatcaacc ccggcgttcc atcaggtaca ggtttcaacg tttcaggatc aagcgctcgc gcatataccc acggcggaac atgtttacgc tgcatttcat cctcaaagaa acaagtgttg agttcaactt gattaaatat atctcggatc tgactaatat cactgagatt gaaagtatca aataaaagat gattgaaatc atcacgtttc agagattctt tttcgtaact tttccagccg cctccggtta tgatataaag gcttttatct ccagaaaatg agattttttt atctttcata taatggcaga gtaaataaat aaagtatggc gaaccaataa gacaaagatc tttcccttga ttttttattc gttcaagact attcaatgtt ttaacaaaat ctattcgttc ttctgttacg gtaaatgtcg taggatataa caattccacc aaactcataa catatttaaa ccaaatatta tgagcattaa atctatctgg tcccaaattg actaattcta tttgatgatc aaaccaacta ccaacatatt tcatgccata actcacagag cctaagagtc tctcaatact taatctgtca cgcgccacct gactttttaa accattcgtg ccgctactgg taaaccaact ttcaatctcg ttttcctgag aagttaataa gcgagtaaac ttaaaaaccg atgttgggaa tacaggtatg tcatcaattt ccgtaatatt gtcatctact ttgtgtgcct gacagtagtg acgatattct cgacaatgtt tataatgatt acgaaatgca tcaagcacaa gtttctttct gattttttcc tgctcgtcgt aagaccacac taatggatcg ctcgaaaaaa tcaaatcatc aatttctgag cttgctgtaa tttcttgttt atcaacatat gaagtcatac ctgttttcct cctcgactta agacagagaa attgcttgat tttcaatctc aattctcatt cggcgttcat tgactgtcgc aatagttaaa tgttcaaatg acggttcagt aatatcaaca tcaatatcca gatgatcatt atccatcgcg atagcggctt tcgtaaccga ttgataaaaa ttgcgcagga ccactaaatt ttcactcaag tcatgcgaac ttcctaacaa agaatatatc ttgcatcga t tactacgaat atttgataac aatgtgataa cttcatcttg cttgacccaa ttatcgttat ttgcagtaaa agcaataaac ggtatatcaa gatacatcat gttattaatt gtagaagcta aatcttccca accaaaatca agacaatctc tcgcaaagac ttcagcaccc aatttatggc cttcaaaatc tagattatcc ggcaattcat taatgggtag actgagataa tcaaacccta aagctctttc aagagaatat cttaagttaa caacaccgac tgcggtgatt aaaaacgaag cattgatttc agataggctt gcataagcta tccgcgcaga taagcttgaa gccaacatac cgaagttatt tatttttcgt gtagttaacc aatcaaccac tgctaacaag ctctgctttc ctatagacat tgtaaattca tcaattgtcc ctgaactcaa tccaacgtgg tgaagcgaat catagcggat cacatgaaat ccattccgcg ataaatattc cgccagacca gcaaaatgat ccatcctgcg ggcaaaacca gacgcaataa taatggcatt ctttctcttt gggctgtttt cttctggcag cgtttcccaa acatgaattt ttttatttcc ttcaacacaa ataacgtggt cgatggtttt atattttgat tcattttcca tacttttact cctcctcgac ttatgggaca aatacaagga acttatcttc ttccaggaat cgagtctgtt ctatttcaac cgcaacatcc ttagccgtat agttagatgg cctttcatga gaaatatatg tcactaatcg ttgcaacggt ctcattccgt catgagatcc accaactcga aatatgttat 6180 6240 6300 6360 6420 6480 6540 6600 6660 6720 6780 6840 6900 6960 7020 7080 7140 7200 7260 7320 7380 7440 7500 7560 7620 7680 7740 7800 7860 7920 7980 8040 8100 8160 8220 8280 8340 8400 8460 8520 8580 8640 8700 8760 8820 8880 8940 9000 9060 9120 9180 9240 9300 9360 9420 9480 9540 9600 9660 9720 3 201142028 tcattcctgc ttctacaatc ctttccgcac cttttaatgc taacgcatct cgatatttaa 9780 atgatgactc ccaaggaaaa atagatatgg tttgcgtctt atttttttga acataaggca 9840 atatttgctc aatattatcg acgtgatgaa ggtacacaca tctgccaagt ggttgattaa 9900 attccacacc tgcatttgac tcaataatca tccaacgttg atgaatatcc acctctactt 9960 ttaatccagc aaacaagctt tctttttgaa ctaaagaata ggccgccttt tcatcaaaat 10020 cttttttggc attcggtaat atatgcgcat atagattaag tttttctatc aacgctaact 10080 taaattcctc ataatgattt Cccatgtaat atatgttttg ggcagaaaaa caagctcgct 10140 gatcgtaaaa acaaacatca tgagccgcac ctgtcgctgc ggacgtcaaa tcaacaggat 10200 tatcgataat gcaaagactc tttttagaac caaatttaat cacatcagca taagatggcg 10260 catgctctac cgcccaatta atcgcatctg gccctcccca agcgacaata acatccgcat 10320 gt cgcataat ttcttttgcg agtgatgtat caccttggtg gggccaatat ataacagata 10380 aagagcgcgt tatcggatga ttagggtcta catcaataaa acttaacgct aatgcattag 10440 cggtaaaagg atcggttgac gatgttttta taatacactg attcttagtt aaaattgcgc 10500 gtaatataga catgatccca gataatggaa cattacctgc caacagatgt acagatttac 10560 ctttcggaaa agcccgaaca taactttcat cctgaggtag ccattcatcc atgatatggc 10620 gagaaccaag ttcattttct acaacatcat aaaggccgcc tttagaacat aaaatcatag 10680 atatccaatt ggcctctagc ttagccattt cttctgaata tcccatatat ttttttaagt 10740 cacgaatgta tgtcctgcgt cttgagtatt cttcattttt ccatctttgc cctaccgtat 10800 agagaaaatt gacaatgtta tgcaaccgta attcgttatt tccattacaa tcaataatgt 10860 tttttacatg agagtcattc aatattggca ggtaaacact attatcacca aaattaatgg 10920 attgcactaa atcatcactt tcgggaaaga tttcaacctg gccgttaata atgaatgaaa 10980 tttttttagt catatttgcc atcctcctcg acttaggtat attccatgtg gtacttctta 11040 atattatcat caacaatatt gattacattt ttttggctca tcaaatcatt cattggttca 11100 aaggacagca atacactttt cgcaccacac ttttcaattg ccaacttagc cgcagttata 11160 cactccgtat aatttccgac agcgttttct gcaattattt cttcaagttt attttcgaaa 11220 ttttcattag ggtgcatttc aagaacataa tcactaataa atgcacgcgt ctcttgttta 11280 gctttattac tatcttcgtt atagttaact aatatcatta actgatggtc tatctctgat 11340 aggtcaacgt catatttatc cgcaacggct ttatatcttt cagcatattc atatctaaca 11400 tcattagaat catcccactt aaagatgaga ggaatacctt ttttggccgc ccactcaaca 11460 atatgatgac tggttgctgt tacatatttc cgaggtccgc ctggcgtata agcatgggga 11520 tttacagata ttttagggaa gctataaaaa tcgttatctg gattacaata gcctgttgtt 11580 aaagcatcgt taatgatttc ataacactct tcaaatagtt gctgttgata ttcaaccggg 11640 cgattaaaaa aatgcatttc atcttttttt tcgcaatcac taaaccctaa aataaatctc 11700 ccttcactta actgatccaa taagcaagct tcctccgcta tggcgacagg atgatgagtt 11760 gtaatgatgt gatttaatga accaatttta attttctctg ttaaaccgag cagaaaacca 11820 gaaacagtca gaggagcgcc gacaacacca ttatctgaaa aatgattttc atacactaaa 11880 atctgttcaa aattcaactt atcaacatac tccgttattt cctgcatgcg aactatactt 11940 tgttcttgaa cagttgttga attgatgaag ttaaggaaga acaatccaaa ttt catttct 12000 ttctcctcct cgacttaata taatagcgaa cgttgttttt ctttaagaaa tggcatgaca 12060 tcagactgga agagcttcat ggaagcaata atttcgtcta ctgttccatt agcttcaaat 12120 ccacaacaaa tatttgatat tcctgtagca tcaatgtctt tttgaattat gtcaatacat 12180 tcctgcggcg ttcccacggg attgatttcg taactgtaat caatacggcg attagtatct 12240 ttatgtcctt ttaatacaaa gtcacgccac tgccctttat tgaaatcata acctcttgtt 12300 tggtctgaat catcaaaaat agtcgtagca ttcacataag aatcatacca atgccccaga 12360 aatttccggc aaatctcttt cgctttaatt gagtcatgat ctacagatgt tatatatgat 12420 aagcaatggt cgatattatg aatatcgtgc ccatattctt gagccacttc attataaagc 12480 tcaagttgtg ctttcttttc gttagtattt ataatccaac ttaatatcat cggtaggcca 12540 aattgagcag cccactcagt cgtcgaagct gattcagcca ccacataaac cggtgcgcca 12600 cctctgctat acgccgcggg gtttactttt accttatgga acttgatatg ttcattatca 12660 gcttccatat atccctctgt catgccattc tttatcagcc cgtaccagca ttccgctaag 12720 gcgcgactgt tattcatatc tgtgccgaat acgcgaaagt ccttgttgta aagccctcgg 12780 caaataccaa accgaaatcg tccttttgac atttgatcca ataaat tcac atcttcaagt 12840 tggcgtactg gatgggctgt gggaagaaca atagcggcag ttcctacatt caatttttta 12900 gtcgcgccaa gtaaatatgc agcagcgaca taagggttac caagcaaacc aaactccgtg 12960 aaatgatgct ccagtaacca tacggtatca aaaccacact cctcagagat gcgacctaat 13020 ttaaccaaac gtttcattac ctctgtttga gaaaattggg gaggttggta tgtaagcaaa 13080 aagtttccaa atttcataga gagtcctcct cttgcttcat ctgcaggcat gcaagcttga 13140 gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt 13200 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata Aagtgtaaag 13260 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 13320

201142028 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttcgata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgg cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata ccgcgcccgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata gtgtatgaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tatttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttgcggtcg aggtgccgta aagctctaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggc gaattcgagc tcggtacccg gggatcctct agagtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg atccactagt tctagaatca gaccatggta cggtgaatat tcaattcgtg cagaaaatct tcctttgtta tcatcaagtt ggtgccaaaa ttcaccatga aaaaagtatt cttttgttag ttcgtatttt tttgttgcca tgtactgctc catttgtttc tgagaatcct tcagaatagt aaaaatacta ctcaaaactc gtcttcaaga tctctggcta tctgctcatt ttcatgatgc tcaacagcat cactgatcaa attcatcatg ttattaagaa actcctctac aactttaaac gcatcattga gcgtagctgt attgaatgta tgtatctttt catccgttgt tttcgttttc ccgttacgat gaactatgtc atgcctcagt ttcatcaatt cattgatatt tttgagtgga aaacgtggat attgcttagg ttgcaggact gctttatata tttcaactac cagttggatt ctatgataaa gaatatctga aaggtactcc tgtacatatt tgtttgcatt agactcttta tttatcaact cagacagaga 13380 13440 13500 13560 13620 13680 13740 13800 13860 13920 13980 14040 14100 14160 14220 14280 14340 14400 14460 14520 14580 14640 14700 14760 14820 14880 14940 15000 15060 15120 15180 15240 15300 15360 15420 15480 15540 15600 15660 15720 15780 15840 15900 15960 16020 16080 16140 16200 16260 16320 16380 16440 16500 16560 16620 16680 16740 16800 16860 16920 201142028 gatattcttt gctttcagtt catttatgtt tcttatggca ttttctacat atctgttatg 16980 tgacaataca acgcttttaa tcatttcgct aagacagttt tccattattg tcacaatgta 17040 tgcgattttc atttttatga aaacttcact tgtagattgt tttccctctt cttttatttg 17100 ttctaaaagg tttattgcta ttttataaat ttctgtatgt ggatgctgtt ttaaccattt 17160 ttcgttttca tattcttcca tagccatatc agaaagaaag tcctgatact cgtcatagtc 17220 cttttcaagt aactgccatt cttcagaatc ctcctcaaga tctgggctgg aaagccgttc 17280 tcgtatccat tcctcacggc gactttcctc gacttgcata acccaatctt ttgtagctcc 17340 cacaataccc tccttatgtt cattgctaaa tatcaatagt cactttatcg gagactacgt 17400 gaaaaatctt ctcctttccc cctgcttttt tatcagattt cacgcagcgt accgccgcta 17460 ctgtatccat caaaaaaact gatagctttt taatcatctt ttgtgctgca acttactgaa 17520 aaaaggatta atggaaacgc cgataacggc attgatctgt tggccaacgc gaccggcagc 17580 iittggactta acaaaaaaac tgaaagaaaa tcccccaaat gaagggaaaa aacggaagaa 17640 cagtcgaacg acaggcacaa aaaaacccga ctggtaatcg ggctttttcg tgttttccag 17700 tcctgagttg gtggctctga ctggaggaag atactctaca tccaacatcc acaatattat 17760 ggcactagac accagttgtc tagtttgact tcagattgtc tgtcaatggc catttcttta 17820 aatgaatttt atgcgtgcat ctgtataatc tttgtgttgg cgctgtacag tgtgcctccc 17880 ttgcttctaa cccctgcgct atctggggat gaacgaatta aagtcctggc tgaacaaaca 17940 ggtggtgctg tgttcagtgc tctggcaggg ggaaggtact ctacatccaa caatgtgaga 18000 gagccgttgt gacaggcgat gtatgagcaa gcaacggtga cttcctccag tatcaaccta 18060 gagaaacgga tgatgaatct gttaatcatc gttctcaggg ctgtgctcgc cattgcaaac 18120 gcgctgattg ccattcagaa actaatcgag tgattgatgg cttaactaag ggagagacgt 18180 ttaccgcttc ctgcccttta acaaaggagt gtttatgagt tttgaacaat tatttacact 18240 aacgacccaa cgtcgttggg tttgccgcag aaaatttatt catttaaaat agatctaaat 18300 gaagcgggaa actgcagacg taaaaaaacc gactggtgag gtcggctttt ttacagttcc 18360 ggtacgagct ggtaactcgc ccggaagaga aactctctac aactgacaac agtatgataa 18420 tccgtgaagc ctgattatgc aatatgacag gcggtgacta cgcgttaacg gtttgcttga 18480 aagttagata aacgtataat cagagggtta gtgctgtggt gagtctttcc ctgcattccg 18540 ctaaatgcgg catgaacagc ttcaaagtcc tgacaggtaa tacaggtggt gctggttatc 18600 tgtgctctgg taggagtgag actctctaca actgacaaga cgaagagaag ccgtggtgac 18660 aggcagcgta agagcaagcc acggtgtctt tactccattt atggagaaaa cggatgatta 18720 acctgttaat catcgttctg agggcggtag ctgcgcttgc aaacgcgctg attgcagttc 18780 tggaactgat ccgtcagttc atcgattgat gactgcggaa acgtaactaa gggcaggaag 18840 tgcaccgctt cctgcccttt aacaaaggag gtgttatgga ttttatgagc attttggggc 18900 gctgtttact tcttcctttc cctgttgcga tgtttgtcgg ttgcctgttt ccaggcatcg 18960 atgatcgttt tactggaatg ttcatgagtt ttattgtcgg ttgtctatcg tggtatatca 19020 ccaagccaaa accgcaaaag gctgaggtcg cgtaagcggc cttttatttt gatacgcatt 19080 agttacaaca ttcaatttgc tttttaaaaa tatccacttc agcacctccc tcataaataa 19140 cgtcaaccag tttattgagc tcaccctacc ctactcaccg gaggattgca gggatttacg 19200 ttttattgcg ctactgattg tgccgcggct gcaccctagt accttctgta cctgtgtcca 19260 ggagctgcca ctttctatca acctgataat tgcatcatgt ctggcttgat taggtttacg 19320 ccctttatat ttgccgtctt tccttgcttt ctctatcccc tgtttctggc gttcccgccg 19380 ttgttcatag tcccttctag caacagcggc cagcatatcc agcagcatat cgttaatggc 19440 tgcaaacaac cggctgtcaa actcactcat accagaatta atccaggtcg tcggcacatt 19500 cacggccata acccggatat ctttctgacg gatcattttc ttcagcgtat tccagtcttc 19560 ccctgacagt cgggaaagtc tgtccacatc ctcgataagc aaaatgtcat tttgctggca 19620 atctctcaga agacggaaga gttccgggcg gtcaagacgg gagccggatt cattctcaat 19680 ataatagctg caaatgatca ggcctctttc cctggcaaaa gcctcgatag tttccagggc 19740 acgcgaagca tcctgttctg ctgttgaagc tctcagataa gcgcggacaa agtttgttgg 19800 tgcatttgaa gtcatattac agaaccagtt cgcataaggt cacaccacta tacccaaaat 19860 gaaccagaaa gtggatcgac aggaaaggca cactctaaaa gaaccgaaaa aacaaataat 19920 gagtaaatgt gacatcgtca cccatactca taaactcaca tgctcatata cacaacaaac 19980 tcaaatgagt atgtgctctt taactcataa actcatatta acctttactc atgcaaagag 20040 tatggtaaac tcaaaaactc acaagagtaa ttgagtagat acacaattga gggtttgagt 20100 atgaaagtaa tctcatttct gaatccgaaa gggggttcag gtaaaacaac tgccgtaatc 20160 aacatagcca ctgcgttgag cagaagcgga tacaacattg ctgtggtaga tacagatcca 20220 caaatgagcc tgacgaactg gagcaaagcg ggcaaggcag catttgacgt atttacagct 20280 gcatctgaaa aagacgtcta tggaatccga aaagatctgg cggactatga ctttgctatt 20340 gtggacgggg caggttcgct ctcagtaatc acctccgcag ccgtcatggt aagcgatctg 20400 gtaattatcc ctgttacacc cagccccctg gatttctccg cagcaggaag cgtcgttact 20460 gttctggaag cacaggctta cagccgcaaa gttgaagccc gctttctgat cacccgtaag 20520201142028 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttcgata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgg cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata ccgcgcccgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata gtgtatgaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa ctgttgagat aactctcaag gatcttaccg cgtgcaccca actgatcttc agcatctttt ccagttcgat gtaacccact actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tatttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttgcggtcg aggtgccgta aagctctaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcgg t cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggc gaattcgagc tcggtacccg gggatcctct agagtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg atccactagt tctagaatca gaccatggta cggtgaatat tcaattcgtg cagaaaatct tcctttgtta tcatcaagtt ggtgccaaaa ttcaccatga aaaaagtatt cttttgttag ttcgtatttt tttgttgcca tgtactgctc catttgtttc tgagaatcct tcagaatagt aaaaatacta ctcaaaactc gtcttcaaga tctctggcta tctgctcatt ttcatgatgc tcaacagcat cactgatcaa attcatcatg ttattaagaa actcctctac aactttaaac gcatcattga gcgtagctgt attgaatgta tgtatctttt catccgttgt tttcgttttc ccgttacgat gaactatgtc atgcctcagt ttcatcaatt cattgatatt tttgagtgga aaacgtggat attgcttagg ttgcaggact gctttatata tttcaactac cagttggatt ctatgataaa gaatatctga aaggtactcc tgtacatatt tgtttgcatt agactcttta tttatcaact cagacagaga 13380 13440 13500 13560 13620 13680 13740 13800 13860 13920 13980 1440 14100 14160 14220 14280 14340 14400 14460 14520 14580 14640 14700 14760 14820 14880 14940 15000 15060 15120 15180 15240 15300 15360 15420 15480 15540 15600 15660 15720 15780 15840 15900 15960 16020 16080 16140 16200 16260 16320 16380 16440 16500 16560 16620 16680 16740 16800 16860 16920 201142028 gatattcttt gctttcagtt catttatgtt tcttatggca ttttctacat atctgttatg 16980 tgacaataca acgcttttaa tcatttcgct aagacagttt tccattattg tcacaatgta 17040 tgcgattttc atttttatga aaacttcact tgtagattgt tttccctctt cttttatttg 17100 ttctaaaagg tttattgcta ttttataaat ttctgtatgt ggatgctgtt ttaaccattt 17160 ttcgttttca tattcttcca tagccatatc agaaagaaag tcctgatact cgtcatagtc 17220 cttttcaagt aactgccatt cttcagaatc ctcctcaaga tctgggctgg aaagccgttc 17280 tcgtatccat tcctcacggc Gactttcctc gacttgcata acccaatctt ttgtagctcc 17340 cacaataccc tccttatgtt cattgctaaa tatcaatagt cactttatcg gagactacgt 17400 gaaaaatctt ctcctttccc cctgcttttt tatcagattt cacgcagcgt accgccgcta 17460 ctgtatccat c aaaaaaact gatagctttt taatcatctt ttgtgctgca acttactgaa 17520 aaaaggatta atggaaacgc cgataacggc attgatctgt tggccaacgc gaccggcagc 17580 iittggactta acaaaaaaac tgaaagaaaa tcccccaaat gaagggaaaa aacggaagaa 17640 cagtcgaacg acaggcacaa aaaaacccga ctggtaatcg ggctttttcg tgttttccag 17700 tcctgagttg gtggctctga ctggaggaag atactctaca tccaacatcc acaatattat 17760 ggcactagac accagttgtc tagtttgact tcagattgtc tgtcaatggc catttcttta 17820 aatgaatttt atgcgtgcat ctgtataatc tttgtgttgg cgctgtacag tgtgcctccc 17880 ttgcttctaa cccctgcgct atctggggat gaacgaatta aagtcctggc tgaacaaaca 17940 ggtggtgctg tgttcagtgc tctggcaggg ggaaggtact ctacatccaa caatgtgaga 18000 gagccgttgt gacaggcgat gtatgagcaa gcaacggtga cttcctccag tatcaaccta 18060 gagaaacgga tgatgaatct gttaatcatc gttctcaggg ctgtgctcgc cattgcaaac 18120 gcgctgattg ccattcagaa actaatcgag tgattgatgg cttaactaag ggagagacgt 18180 ttaccgcttc ctgcccttta acaaaggagt gtttatgagt tttgaacaat tatttacact 18240 aacgacccaa cgtcgttggg tttgccgcag aaaatttatt catttaaaat agatctaaat 18300 gaa gcgggaa actgcagacg taaaaaaacc gactggtgag gtcggctttt ttacagttcc 18360 ggtacgagct ggtaactcgc ccggaagaga aactctctac aactgacaac agtatgataa 18420 tccgtgaagc ctgattatgc aatatgacag gcggtgacta cgcgttaacg gtttgcttga 18480 aagttagata aacgtataat cagagggtta gtgctgtggt gagtctttcc ctgcattccg 18540 ctaaatgcgg catgaacagc ttcaaagtcc tgacaggtaa tacaggtggt gctggttatc 18600 tgtgctctgg taggagtgag actctctaca actgacaaga cgaagagaag ccgtggtgac 18660 aggcagcgta agagcaagcc acggtgtctt tactccattt atggagaaaa cggatgatta 18720 acctgttaat catcgttctg agggcggtag ctgcgcttgc aaacgcgctg attgcagttc 18780 tggaactgat ccgtcagttc atcgattgat gactgcggaa acgtaactaa gggcaggaag 18840 tgcaccgctt cctgcccttt aacaaaggag gtgttatgga ttttatgagc attttggggc 18900 gctgtttact tcttcctttc cctgttgcga tgtttgtcgg ttgcctgttt ccaggcatcg 18960 atgatcgttt tactggaatg ttcatgagtt ttattgtcgg ttgtctatcg tggtatatca 19020 ccaagccaaa accgcaaaag gctgaggtcg cgtaagcggc cttttatttt gatacgcatt 19080 agttacaaca ttcaatttgc tttttaaaaa tatccacttc agcacctccc tcataaataa 1 9140 cgtcaaccag tttattgagc tcaccctacc ctactcaccg gaggattgca gggatttacg 19200 ttttattgcg ctactgattg tgccgcggct gcaccctagt accttctgta cctgtgtcca 19260 ggagctgcca ctttctatca acctgataat tgcatcatgt ctggcttgat taggtttacg 19320 ccctttatat ttgccgtctt tccttgcttt ctctatcccc tgtttctggc gttcccgccg 19380 ttgttcatag tcccttctag caacagcggc cagcatatcc agcagcatat cgttaatggc 19440 tgcaaacaac cggctgtcaa actcactcat accagaatta atccaggtcg tcggcacatt 19500 cacggccata acccggatat ctttctgacg gatcattttc ttcagcgtat tccagtcttc 19560 ccctgacagt cgggaaagtc tgtccacatc ctcgataagc aaaatgtcat tttgctggca 19620 atctctcaga agacggaaga gttccgggcg gtcaagacgg gagccggatt cattctcaat 19680 ataatagctg caaatgatca ggcctctttc cctggcaaaa gcctcgatag tttccagggc 19740 acgcgaagca tcctgttctg ctgttgaagc tctcagataa gcgcggacaa agtttgttgg 19800 tgcatttgaa gtcatattac agaaccagtt cgcataaggt cacaccacta tacccaaaat 19860 gaaccagaaa gtggatcgac aggaaaggca cactctaaaa gaaccgaaaa aacaaataat 19920 gagtaaatgt gacatcgtca cccatactca taaactcaca tgctcatata caca acaaac 19980 tcaaatgagt atgtgctctt taactcataa actcatatta acctttactc atgcaaagag 20040 tatggtaaac tcaaaaactc acaagagtaa ttgagtagat acacaattga gggtttgagt 20100 atgaaagtaa tctcatttct gaatccgaaa gggggttcag gtaaaacaac tgccgtaatc 20160 aacatagcca ctgcgttgag cagaagcgga tacaacattg ctgtggtaga tacagatcca 20220 caaatgagcc tgacgaactg gagcaaagcg ggcaaggcag catttgacgt atttacagct 20280 gcatctgaaa aagacgtcta tggaatccga aaagatctgg cggactatga ctttgctatt 20340 gtggacgggg caggttcgct ctcagtaatc acctccgcag ccgtcatggt aagcgatctg 20400 gtaattatcc ctgttacacc cagccccctg gatttctccg cagcaggaag cgtcgttact 20460 gttctggaag cacaggctta cagccgcaaa gttgaagccc gctttctgat cacccgtaag 20520

201142028 atagaaatgg caaccatgct caatgtgctg aaagaaagta tcaaagacac tggtgttaaa tctttccgta cggccattac acaacgtcag gtttacgtga aatcaattct ggatggtgac agcgtgtttg aatccagtga tggcgcagca aaaggtgaaa tagaaatcct tacaaaagag atagttagca catttgagta attactcatt cactcatata atcaaataag tataacaacc ggagtaacct taatgtcact tgaaaaagcg catacggcag taaaaaaaat gacctttggt gaaaacagag atctggaacg agtagtaaca gcaccagtat catctggaaa aatcaaacgt gttaacgtca attttgacga agaaaaacac acccggttta aggctgcatg tgccaaaaaa ggtacatcga tcacagatgt ggtgaaccag cttgtagata aatggctcaa agagaacgaa taatatctga ggatatatca tggataaaaa cgtcgttgat gcgctgaaaa cgctattaga agcgttaccg gaagaggtgg taacagaagt cacatcaaaa ctaaatcctt cggcaagcca tattcctgaa gaaaacagta agcaattgac agcaaaagca agactactga atttccggct aaccgaagcc tatgaagaaa tcctggaagt cgaagctatc agaacaggcc agagcaaaac taccgttcta aaggcagcac tggcgatgta caacagccag gatgaaaaca taaagaatca ctggctactt gagtctgcaa aaattaacta attcaggjata tagattatta gtaggagttc gggcaataat ggatgataac tctacgtcag taaccagcaa agaagatgtg caggatattt ctttaaaaaa ctggctaaag aataaaaaaa ccattgtatt acttatattt actgcattaa caaatgttgg caatgtcctt tcgacgattg atattatcac agataaaacc agttcttttt acacatggct tggggaatcg aaaaaattcg aaggccattg gaccaacaat acagaaggat tcatagacgg cactcctgat tttttactca agaatgcagg cgatgtcctc ataaaatttg acctgaacat caaaggtggc gaagtgaggg gagagcttca caccgacgca cgactaaaaa tgtgtgaaac tatagaagaa aagttaaaaa ctttgtgtac attaatagcc tcacatccac taatgattga gggggaaaaa tcaccatttt ccaatgaatt cgatgcctat attactgagt atagaaatgg agataaaaaa atagtcgcaa tattaaatat gaaaattaca gacgatgaca aaatgacaat aacaaataca atgagaacac cagaatcagc attattttca actaaaatat atgctataaa aataataacc gaagaataac ttaataacaa aaaaggtatt aaagtgaata ctggaataac aattgatttg accaaccttt cagaagatga attgcttgat ttatattcaa tgtataaaag tgcaaacata gcacatcaac tatggtgcag acgccatgaa aatataccag agcatttttc gataatattc gtgacgcttt tagaacgaat aaaaagggtt acagaaaaga actcagaagg ggtaaaaaca ccagatgtag acctggatgc attaattgac accatttata ttggttgtcg ttcaatgttc tgtgaaaacc ctggcttaaa aaataactac actctgcaaa actgcctgag gaaagccaat tatcacaacg aagctagagt gatagataat attttacagg aaaaaaaatt cacagattcc atcatgaaag atgagtcatt ttttagtctg gtgaaattag tttccaataa gtccattgca caccaggaga gcctttcagg aaaaaaacgg gaaaagatag actatcgata taaattctta aatgacaatt caaatatctg tgagtttcag tattacattt ttagatgtca ccgtatttat gagaatattg tgaaagaata tggggataca ttactgaatg agcttaaaat aaaaaacaat gatatataaa gaatgccccc atatgaatag aaggaagaat catactaata aattttccag cttatcgctg gtaagcaatt ctgccagcga atcacatttc ataaaatcaa tatccatttc gtccagaaca tcacagtagc tgacatcatc aagactaatg acaatcacag gaacaccctc tttgagatca aacactaaga caggtaatga aatggagttt atcttttcaa aatcactaaa aaactgttca gttccatatt cactggcagg aaaaagaatc tcacttaatc tcacacaata aaaaacatga tagtgttctt taaggttatc ctttaaaata ttaacagcct tagtatattt ttcgttaata aactctttga ccaaatattt cataaaactc tccaacaaga accgactgta ggtcatcggg caaacgttgc ggaatggcgt cagagacgtc attttgcggc gtttgcccta tcctgcatcg cagtggcatc atttctggct tatctcgcta ctgttctcgg tgctttcgtc cgcaggttcg ggaacaagta acttgtgcca gggcatagtt ttgttgagca gctccatcag aggctcagcc agcagtccca aaatccaggt tgccgtaatc accggtatag tgagattttg tggcgtccat aactggtgat cgagatgata ccaggtgatg acacacaaaa taccacccag ccagaacaca gcccatttaa acgcaccaag aatggctaac acaaaaacaa agataaaatg taaaacactc gcggcaagaa gacgaagata aaaccatccc cagtcaagcc acttaacaaa agtgctccct gttttcactg caaaacgttt cttacgctcc ctggcgattt ctttcctgaa tttagcacgt tcttccccct gagggaattg atagatttta gccatgagtt acctcatttc gatctcaaaa cgaagtttat gcaattatcc tctgaaaccg tatggtattc tgatatctag gccttcactt tctttatcta ttttataaga aaaccttagc tttgatgctc ttcttgcgac tctctcaacg attttaactt cgagatgatt aatctctgta tattgattta ttttttcaat gtttttttgc agaaaaactt tattaaagtc cttaaaattc tgataaaggt aaactttttc gccattatct attctgaata attcaagttc atcctttaat acatctactt caatgtcaaa aaatgtcttt ttaccactgc tgtattgttt cctgataaac tgataaagca tatttgaatt ttgatcagca agtcttacag acgatatcaa aacctgagta gtgtaagaat cctttaacat cgtgatatat ggctcaatga catgtgaaaa agcaatatca atatatcctt cactttcaac ataatcacaa aacgcagtca ggttcataag cctgactgca 20580 20640 20700 20760 20820 20880 20940 21000 21060 21120 21180 21240 21300 21360 21420 21480 21540 21600 21660 21720 21780 21840 21900 21960 22020 22080 22140 22200 22260 22320 22380 22440 22500 22560 22620 22680 22740 22800 22860 22920 22980 23040 23100 23160 23220 23280 23340 23400 23460 23520 23580 23640 23700 23760 23820 23880 23940 24000 24060 24120 201142028 tcagaaggta atttacctct cttcttcgta tgtccagaga tttgattgcc ctgagctgtg 24180 agaagttgat ttcgaggaat gcgaataaca ctggcctgaa gttcttctgc cccctctttc 24240 aactgccggt aagcacctgt tacatcaata tcagcgataa atgcatattc ccgagccgta 24300 atacgaaagg ttgccccctc agataactct tcacgtgaat caatctgcgc catagccata 24360 aacaaaattc ttctggcaga caaaggcaac gatgagaatg tgctgttaat ttcatttctg 24420 tggcgaattt ttgttttctt tgtaacgctc agtaagttca tacattacct gtggttttta 24480 agttctaatg gtgaaatcat aaccacaaag gcggaaatat gtaaagtggt ttatccacct 24540 tatgtatttc caccaaaaca cggatcacat gaaagaattt ttatcattaa tattcaatat 24600 gatagaaatg cgcaggtatc caccttttaa cgtcatacgt tagattagag cactataaag 24660 aactaaagaa atgaagatac aaagatacga atatctgaat aaaaacagga aaatgcgtac 24720 ccatccacct ttcagtgcgt acccatccac ctttcagtgc gcagccatcc acctttcatg 24780 aaaattacag tgcgtaccca tccacctttc agtgcgtacc catccacctt tcattttgag 24840 gcgacaaaaa acaacaccta tcttaatgaa attattgata aaaaaactgg cacaatgttt 24900 gcagaataag ttaaagtaag ttaaagatct taaagtagat ctaccgatct acatttaaga 24960 tcttaaaaac aagaagttta ataagcaaaa aaacacaaaa aatctgtgga taactttgct 25020 caaaacaaaa aattcacact acctgtggat aactttgcgt aaacccggag gacagatcac 25080 tctgaaccaa gacaaccaca cataaaaaca atatgctcac tttttaacca ctgcgcgtaa 25140 ttgcctgatg ttatccttgt gctgttccgc ttcctcgctc actaactcgc tacgctcggt 25200 cgttcggctg cggcgagcgg tgtctccttt ctcaaaacca gaagagttca gatcatacga 25260 tccgttgatt gatccttttt ggatctttca ctgagaagcc ctgaatcgct ttctgtcgct 25320 tcgttcgata caaccatggt gaaaccatgc gaaatcgaaa aaatcgctca gaatgcgata 25380 cagcgcgttt tagagggtat ttacaaaaca gtcgttgcct tgaggaaatt ttgatcaaga 25440 ttgaactcct atccggatca tggcaacaga tcacagcaac aaggccgcta aaaagcgttg 25500 agcggcgtta aacaccgtca aaccgtagcc gactacaacg aaacgggaaa aacgctcaga 25560 acgcgttaca gagcgtttta aggcgatgtc ggaaagagcc atatctcacc tgttctgatg 25620 tttttctcaa catgagcact tttaagagtg cttttaaaga tgctattatg ccaatgagac 25680 aagcggaggg cttatgagct atcaaattct gacaaccaca gcggccagta ttactgacct 25740 gaaaaaaaat cctatgggaa ccgtagctga aggtgaaggg gacgctgttg cgatcctgaa 25800 ccgaaacgaa ccggcgttct attgcgttcc accaaaactt tacgcctact atcgggaact 25860 cgctgaagat gctgagttaa acgctgttgc tgatgagcgc atgaaaaacc cggaaattgt 25920 gaaggttaac ctggatgacc tatgaactgg cttttgaccg tagagcactg aaggaatggc 25980 agaaactcgg ccacaccatc cgtgaacaat tcaaaaagaa actggcagaa cggctggaaa 26040 atccacgcgt acccgcagcc cggttacatg gtcatgctga tcgctataaa atcaaacttc 26100 gtgcatctgg ctacagactt gtatatcaag tcattgatga gaaagtcgtt ttacttgtta 26160 tttccgttgg aagaagggaa agcagcgaag tctatcagat cgcagatttg cgctaagatt 26220 aatcatcaaa ggattaactg tttactgtca gaacaagagc attaaaaatt tgccacacct 26280 gcaaatgcag gtataataaa acacaagaaa gggaaacagg ttctctttca tccagagccc 26340 aaagtgggcg ggaggttaaa aatgcaaaac tacgctaagt ctgtagccac agagatttta 26400 cgtcaacttg gtggtaatcg ttttattgtt atgactggtg ctaaaagttt ttcttacttt 26460 gatgaaaacg gtgagtgcgg gttaactttc cgtttgccgt ccaattttgc aatgaaaggc 26520 atcaacttag taaaaattaa actggatttt actgatacgt accaggtgaa attttctcgt 26580 gtacggggtg atgaagttaa agatatttca agattcgata atatctattg tgatcagtta 26640 gcgtgtttat ttacacaaga aacagggtta cataccgtgt tatagataaa aaggggcatt 26700 aagccccttt tttagttatt atgaggaata atatggataa agataagata attaagaaaa 26760 acagaggtaa ttactcatat gtaatcagaa cgatggatga agatggggat gcggtttttc 26820 acgtcttaaa atatgttaag acgattgata aaactaaaag caggaaaaca gtaagaaaat 26880 tgataatgga cgaaaaactc aacctggcat cattgatgct tctggataat gggattttgt 26940 gtgattgtct gacaaaaggg gatgaaaatg cagaataaac ctacacctga agaagtaaag 27000 aatgcacggg ttgcggcagg tcttactctt aaagaagctg ctgatatttt tggttatcaa 27060 ctgaattcct ggcagatgaa agaaagtgca ggtaaggcca gtcgttcttt atctgttggt 27120 gaatatcagt atttattgct attagcaaat atgcatccgt cttacaggct ggtaaaaaaa 27180 taacttgcta tacctgcaaa tgcaggtata ataaaacaca agaaagggaa acaggttctc 27240 tttcatccag agcccaagct gggcgggagc taaaaatgaa agtattaact tttaaaaatg 27300 atactgtctc tgttggtgat gtctttgtat catcctgggg ctatgagcaa accaatgtaa 27360 ccttctacca ggttctttct gttcacggta aaaaaaccgt caccgttcgc gagattcgcg 27420 ctaattcaga atataccgat tcaatggtcg gctttaaaac tcccgtttta aatgatttta 27480 ctggtgaatg ttttaagcgc cagataaaag attttggtga tgagctggca atcaaaatcg 27540 aagattttga aactgcgtat aaaactctac cggaagaaaa acatcgattt tcttcttact 27600 actgattaat cagggggata ttcccccttt tagtaaaggt ctgaaaatgg aaagagagtt 27660 tagcgcaaaa gcatcattaa accgaaatat aaaattttgg tttgagcaat gtgggttatc 27720201142028 atagaaatgg caaccatgct caatgtgctg aaagaaagta tcaaagacac tggtgttaaa tctttccgta acaacgtcag cggccattac ggtgaaccag cttgtagata aatggctcaa gtttacgtga aatcaattct ggatggtgac agcgtgtttg aatccagtga tggcgcagca aaaggtgaaa tagaaatcct tacaaaagag atagttagca catttgagta attactcatt cactcatata atcaaataag tataacaacc ggagtaacct taatgtcact tgaaaaagcg catacggcag taaaaaaaat gacctttggt gaaaacagag atctggaacg agtagtaaca gcaccagtat catctggaaa aatcaaacgt gttaacgtca attttgacga agaaaaacac acccggttta aggctgcatg tgccaaaaaa ggtacatcga tcacagatgt agagaacgaa taatatctga ggatatatca tggataaaaa cgtcgttgat gcgctgaaaa cgctattaga agcgttaccg gaagaggtgg taacagaagt cacatcaaaa ctaaatcctt cggcaagcca tattcctgaa gaaaacagta agcaattgac agcaaaagca agactactga atttccggct aaccgaagcc tatgaagaaa tcctggaagt cgaagctatc agaacaggcc agagcaaaac taccgttcta aaggcagcac tggcgatgta caacagccag gatgaaaaca taaagaatca ctggctactt gagtctgcaa aaattaacta attcaggjata tagattatta gtaggagttc gggcaataat ggatgataac tctacgtcag taaccagcaa agaagatgtg caggatattt ctttaaaaaa ctggctaaag aataaaaaaa ccattgtatt acttatattt actgcattaa caaatgttgg caatgtcctt tcgacgattg atattatcac agataaaacc agttcttttt acacatggct tggggaatcg aaaaaattcg aaggccattg gaccaacaat acagaaggat tcatagacgg cactcctgat tttttactca agaatgcagg cgatgtcctc ataaaatttg acctgaacat caaaggtggc gaagtgaggg gagagcttca caccgacgca cgactaaaaa tgtgtgaaac tatagaagaa aagttaaaaa ctttgtgtac attaatagcc tcacatccac taatgattga gggggaaaaa tcaccatttt ccaatgaatt cgatgcctat attactgagt atagaaatgg agataaaaaa atagtcgcaa tattaaatat gaaaattaca gacgatgaca aaatgacaat aacaaataca atgagaacac cagaatcagc attattttca actaaaatat atgctataaa aataataacc gaagaataac ttaataacaa aaaaggtatt aaagtgaata ctggaataac aattgatttg accaaccttt cagaagatga attgcttgat ttatattcaa tgtataaaag tgcaaacata gcacatcaac tatggtgcag acgccatgaa aatataccag agcatttttc gataatattc gtgacgcttt tagaacgaat aaaaagggtt acagaaaaga actcagaagg ggtaaaaaca ccagatgtag acctggatgc attaattgac accatttata ttggttgtcg ttcaatgttc tgtgaaaacc ctggcttaaa aaataactac actctgcaaa actgcctga g gaaagccaat tatcacaacg aagctagagt gatagataat attttacagg aaaaaaaatt cacagattcc atcatgaaag atgagtcatt ttttagtctg gtgaaattag tttccaataa gtccattgca caccaggaga gcctttcagg aaaaaaacgg gaaaagatag actatcgata taaattctta aatgacaatt caaatatctg tgagtttcag tattacattt ttagatgtca ccgtatttat gagaatattg tgaaagaata tggggataca ttactgaatg agcttaaaat aaaaaacaat gatatataaa gaatgccccc atatgaatag aaggaagaat catactaata aattttccag cttatcgctg gtaagcaatt ctgccagcga atcacatttc ataaaatcaa tatccatttc gtccagaaca tcacagtagc tgacatcatc aagactaatg acaatcacag gaacaccctc tttgagatca aacactaaga caggtaatga aatggagttt atcttttcaa aatcactaaa aaactgttca gttccatatt cactggcagg aaaaagaatc tcacttaatc tcacacaata aaaaacatga tagtgttctt taaggttatc ctttaaaata ttaacagcct tagtatattt ttcgttaata aactctttga ccaaatattt cataaaactc tccaacaaga accgactgta ggtcatcggg caaacgttgc ggaatggcgt cagagacgtc attttgcggc gtttgcccta tcctgcatcg cagtggcatc atttctggct tatctcgcta ctgttctcgg tgctttcgtc cgcaggttcg ggaacaagta acttgtgcca gggcatagtt ttgttgagca gctccatc ag aggctcagcc agcagtccca aaatccaggt tgccgtaatc accggtatag tgagattttg tggcgtccat aactggtgat cgagatgata ccaggtgatg acacacaaaa taccacccag ccagaacaca gcccatttaa acgcaccaag aatggctaac acaaaaacaa agataaaatg taaaacactc gcggcaagaa gacgaagata aaaccatccc cagtcaagcc acttaacaaa agtgctccct gttttcactg caaaacgttt cttacgctcc ctggcgattt ctttcctgaa tttagcacgt tcttccccct gagggaattg atagatttta gccatgagtt acctcatttc gatctcaaaa cgaagtttat gcaattatcc tctgaaaccg tatggtattc tgatatctag gccttcactt tctttatcta ttttataaga aaaccttagc tttgatgctc ttcttgcgac tctctcaacg attttaactt cgagatgatt aatctctgta tattgattta ttttttcaat gtttttttgc agaaaaactt tattaaagtc cttaaaattc tgataaaggt aaactttttc gccattatct attctgaata attcaagttc atcctttaat acatctactt caatgtcaaa aaatgtcttt ttaccactgc tgtattgttt cctgataaac tgataaagca tatttgaatt ttgatcagca agtcttacag acgatatcaa aacctgagta gtgtaagaat cctttaacat cgtgatatat ggctcaatga catgtgaaaa agcaatatca atatatcctt cactttcaac ataatcacaa aacgcagtca ggttcataag cctgactgca 20580 20640 20700 20760 20820 20880 20940 21000 21060 21120 21180 21240 21300 21360 21420 21480 21540 21600 21660 21720 21780 21840 21900 21960 22020 22080 22140 22200 22260 22320 22380 22440 22500 22560 22620 22680 22740 22800 22860 22920 22980 23040 23100 23160 23220 23280 23340 23400 23460 23520 23580 23640 23700 23760 23820 23880 23940 24000 24060 24120 201142028 tcagaaggta atttacctct cttcttcgta tgtccagaga tttgattgcc ctgagctgtg 24180 agaagttgat ttcgaggaat gcgaataaca ctggcctgaa gttcttctgc cccctctttc 24240 aactgccggt aagcacctgt tacatcaata tcagcgataa atgcatattc ccgagccgta 24300 atacgaaagg ttgccccctc agataactct tcacgtgaat caatctgcgc catagccata 24360 aacaaaattc ttctggcaga caaaggcaac gatgagaatg tgctgttaat ttcatttctg 24420 tggcgaattt ttgttttctt tgtaacgctc agtaagttca tacattacct gtggttttta 24480 agttctaatg gtgaaatcat Aaccacaaag gcggaaatat gtaaagtggt ttatccacct 24540 tatgtatttc caccaaaaca cggatcacat gaaagaattt ttatcattaa tattcaatat 24600 gatagaaatg cgcaggtatc caccttttaa cgtcatacgt tagattagag cactataaag 24660 aactaaagaa atgaagatac aaagatacga atatctgaat aaaaacagga aaatgcgtac 24720 ccatccacct ttcagtgcgt acccatccac ctttcagtgc gcagccatcc acctttcatg 24780 aaaattacag tgcgtaccca tccacctttc agtgcgtacc catccacctt tcattttgag 24840 gcgacaaaaa acaacaccta tcttaatgaa attattgata aaaaaactgg cacaatgttt 24900 gcagaataag ttaaagtaag ttaaagatct taaagtagat ctaccgatct acatttaaga 24960 tcttaaaaac aagaagttta ataagcaaaa aaacacaaaa aatctgtgga taactttgct 25020 caaaacaaaa aattcacact acctgtggat aactttgcgt aaacccggag gacagatcac 25080 tctgaaccaa gacaaccaca cataaaaaca atatgctcac tttttaacca ctgcgcgtaa 25140 ttgcctgatg ttatccttgt gctgttccgc ttcctcgctc actaactcgc tacgctcggt 25200 cgttcggctg cggcgagcgg tgtctccttt ctcaaaacca gaagagttca gatcatacga 25260 tccgttgatt gatccttttt ggatctttca ctgagaagcc ctgaatcgct ttctgtcgct 25320 tcgttcgata caaccatggt gaaaccatgc gaaatcgaaa aaatcgctca gaatgcgata 25380 cagcgcgttt tagagggtat ttacaaaaca gtcgttgcct tgaggaaatt ttgatcaaga 25440 ttgaactcct atccggatca tggcaacaga tcacagcaac aaggccgcta aaaagcgttg 25500 agc ggcgtta aacaccgtca aaccgtagcc gactacaacg aaacgggaaa aacgctcaga 25560 acgcgttaca gagcgtttta aggcgatgtc ggaaagagcc atatctcacc tgttctgatg 25620 tttttctcaa catgagcact tttaagagtg cttttaaaga tgctattatg ccaatgagac 25680 aagcggaggg cttatgagct atcaaattct gacaaccaca gcggccagta ttactgacct 25740 gaaaaaaaat cctatgggaa ccgtagctga aggtgaaggg gacgctgttg cgatcctgaa 25800 ccgaaacgaa ccggcgttct attgcgttcc accaaaactt tacgcctact atcgggaact 25860 cgctgaagat gctgagttaa acgctgttgc tgatgagcgc atgaaaaacc cggaaattgt 25920 gaaggttaac ctggatgacc tatgaactgg cttttgaccg tagagcactg aaggaatggc 25980 agaaactcgg ccacaccatc cgtgaacaat tcaaaaagaa actggcagaa cggctggaaa 26040 atccacgcgt acccgcagcc cggttacatg gtcatgctga tcgctataaa atcaaacttc 26100 gtgcatctgg ctacagactt gtatatcaag tcattgatga gaaagtcgtt ttacttgtta 26160 tttccgttgg aagaagggaa agcagcgaag tctatcagat cgcagatttg cgctaagatt 26220 aatcatcaaa ggattaactg tttactgtca gaacaagagc attaaaaatt tgccacacct 26280 gcaaatgcag gtataataaa acacaagaaa gggaaacagg ttctctttca tccagagccc 2 6340 aaagtgggcg ggaggttaaa aatgcaaaac tacgctaagt ctgtagccac agagatttta 26400 cgtcaacttg gtggtaatcg ttttattgtt atgactggtg ctaaaagttt ttcttacttt 26460 gatgaaaacg gtgagtgcgg gttaactttc cgtttgccgt ccaattttgc aatgaaaggc 26520 atcaacttag taaaaattaa actggatttt actgatacgt accaggtgaa attttctcgt 26580 gtacggggtg atgaagttaa agatatttca agattcgata atatctattg tgatcagtta 26640 gcgtgtttat ttacacaaga aacagggtta cataccgtgt tatagataaa aaggggcatt 26700 aagccccttt tttagttatt atgaggaata atatggataa agataagata attaagaaaa 26760 acagaggtaa ttactcatat gtaatcagaa cgatggatga agatggggat gcggtttttc 26820 acgtcttaaa atatgttaag acgattgata aaactaaaag caggaaaaca gtaagaaaat 26880 tgataatgga cgaaaaactc aacctggcat cattgatgct tctggataat gggattttgt 26940 gtgattgtct gacaaaaggg gatgaaaatg cagaataaac ctacacctga agaagtaaag 27000 aatgcacggg ttgcggcagg tcttactctt aaagaagctg ctgatatttt tggttatcaa 27060 ctgaattcct ggcagatgaa agaaagtgca ggtaaggcca gtcgttcttt atctgttggt 27120 gaatatcagt atttattgct attagcaaat atgcatccgt cttacaggct ggta aaaaaa 27180 taacttgcta tacctgcaaa tgcaggtata ataaaacaca agaaagggaa acaggttctc 27240 tttcatccag agcccaagct gggcgggagc taaaaatgaa agtattaact tttaaaaatg 27300 atactgtctc tgttggtgat gtctttgtat catcctgggg ctatgagcaa accaatgtaa 27360 ccttctacca ggttctttct gttcacggta aaaaaaccgt caccgttcgc gagattcgcg 27420 ctaattcaga atataccgat tcaatggtcg gctttaaaac tcccgtttta aatgatttta 27480 ctggtgaatg ttttaagcgc cagataaaag attttggtga tgagctggca atcaaaatcg 27540 aagattttga aactgcgtat aaaactctac cggaagaaaa acatcgattt tcttcttact 27600 actgattaat cagggggata ttcccccttt tagtaaaggt ctgaaaatgg aaagagagtt 27660 tagcgcaaaa gcatcattaa accgaaatat aaaattttgg tttgagcaat gtgggttatc 27720

201142028 taaagaaaga gttattcgtt gtattgataa ctggtatgac cttgcatacc caccatcaga acaggagaaa gcaaaaaaag aagcaattga gaagttaata aagtaaagat aaagatggta tcaggttgtg gggcaacctg ataccgtgac acctgattct attgcgagga ataaaatgtc ggaacgcata ttatcagcaa taaatgacgt tgaaaagggt gggcgtccgg ttttcccttt gatgccattc catgtctttc ctgagtatat ggcattactc agaaaagcac tggaaaaaaa gacacaaaag agaacagata aataaaccgg aggctttatg acaaaacatg attttgtgtc ctttgtcagt ggagaattgc ggcaaggcgc tgtacgcttt tctcttgctt ttaacagcaa aggagaaatt gttctgcact ggactaataa agcaggaata cgagtatggc ggatattaag tggtaatcgt ggcaaaaaac caagcaaagc taaccttgaa agaatgagta acttccggcg ctggcttttt gatgcccgtc agggcatgga aggctacact cagcaacctg aacagagcaa ccttagctga atagccgaag ccctcccgta tttcgggagg tgctttctga tatattgcgc gggactgcgc ttgcgcttcc tgccagaccg ttcgcggagc gaacctgacg gcactgtcct taccgcattg atcgggtttc cggtcgcaga cgtacgtatg ttcagccatg acaacatcag cctttttgca tttttgttga tgctcaagca caatttatga gcggtgccag attcactgcg atgcaggata gggcaaacgc cgcaaaatga cgtctctgac gccattccgc aacgtttgcc cggtgaccta cagtcggtta ttgtcggaga attttatgaa aaaagttcaa ttcagaattg atgaaaatca gcataatgat ttgctggatt gtcttaaaac tctttatcca gatgaaccag ctttaacagt agctaaaggc atgaaacttt tagcaaatgc tttattaaaa agtaaagctg gcagtaagga cataaatacg ttttttgata ataatgattt tatcaaaaca acgatgtact taacaggtaa acaaagggct gatattgaaa gagctgctaa tcgtcacgga tggacgttat cacgagaatg tcgttaccgc atacagacga cacttgaaaa tgaactggat ttctttgacc aggaactgct gatgatgaat cgttgccgta attcaattga taagatcggt cgtaatttcc attatatcat tgttaatgat cagaccaggg ttcttgataa agatggtttc tatcaggatg cggagcgtct tacaacagaa atttttaatc ttaagaatca gtttgagaat tacattatgt tatgtaaagg gagaactgtt tcaaataaag tggagatgta attatgggcg tttacgttga taaagaatat cgtgttaaac gaaagtcatc agaaaatggt cgtaagtcag ctttcgctca caaagtcaaa aatggtggaa agaactatag ccgcaatgtt caggaacgta tcaaccgcaa gggtgccagt aaggaggttg ttgtcaaaat atctggaggt gctgttactc gtcaggggat tcggaacagt attgattata tgagccgtga gtcagagcta ccagtgatga gtgaaagcgg tcgggtatgg atgggtgccg aaattctgga ggctaaagag cacatgatag atcgtgctaa tgatcctcag catgtgatga atgataaagg taaagaaaat aaaaaaatca cacagaatat tgtcttctcg cctccagttt tagcgaaagt aaagcctgaa gatttgttgg agtctgtcag gaaaacgatg cagaaaaaat atcctaatca ccgttttgtt cttggatacc actgtgacaa gaaagaacat cctcacgttc atgttgtttt tcgtatccga gataatgacg gtaaacgcgc tgatatcagg aaaaaagatt tacgggaaat tcgtacaggt ttttgtgaag agttgaagtt aaaaggttat gacgttaaag cgacccataa gcaacagcat ggacttaatc agtctgttaa agatgcacat aatacagcac caaaaagaca gaaaggtgtt tatgaggttg ttgatattgg ctatgaccat tatcagaacg ataaaacaaa gtctaagcaa cattttataa agctaaagac tcttaacaag ggggttgaga aaacatactg gggggctgat tttggggact tatgttcgcg ggaaagtgtt aaagcaggtg atcttgtcag gctgaagaaa cttggtcaga aagaagtaaa aatcccggcg ctcgataaaa acggtgttca gcatggctgg aaaacggttc acagaaatga gtggcagtta gaaaatctgg gggttaaggg cgtagacaga acaccttcag ccagcaaaga gctggtactt aacagccctg atatgctgct gaagcaacaa cagcgaatgg cgcagtttac gcagcagaaa gcatccacgt tacagtcaga acagaagctg aaaacaggga ttaagttttt gggcttataa gatttaatgt attgattatg cgagaaaaat atttatttta gttcgcttgc tcatcttttc accttaaaaa cagatcgaaa ttctgcgttt tagctatcat tcgatctatc gatctaaaaa acagatctgc aaaagatgaa tattctgtgt ttcattgcat acatggaata aatgaaagta aaattgattt atcaagatcg aaagatctgt tttatgtgtt gaacttagca taagccactt gtagtataaa cttcgcgcaa tagtaaaaag tctctcttca taggggcgtg atttctcaca agcaaccacg gttggcttgt gttttgtcac atctgtatat cggggattga gtatgaaagg acagaaacag ttttacctgc ttcagtgttc tccgcatttt catcagtcgg ttctgttgca gttatcccgg agcggtatcg cttactacaa tccagttata cgcactttct ataagaggcg tgactgtagt gcatacaggg gacaaatcat gccaatgttt cccggttatg tattcgttct tctggatttt gaggttgtac atccatcctg ctttacccgg atgaaaaatg tttatggact ggtcagtttt ggtgaatatc ctgctgaagt tcctctgagt gtcataaatg aagtaaaaga gcaggagaag attttttcga tgaatttaag cctgatgaat aactcctgtc tggcaaaaat acttctcctt gcagacgctt caaagcgtgg acgagtcttt gccgactatg tgtgtaacag aaaatacgac aggttaaaaa atgacagcta taaaaggaaa gcggaaaccg caacgcaatg tactctacct tcctacagaa gttcgtgttg aagttgaaaa aattgcaatt gaaataagct tcaagagggg gcgacgtatc tctgattctg gttttgttca gtatcttatt 27780 27840 27900 27960 28020 28080 28140 28200 28260 28320 28380 28440 28500 28560 28620 28680 28740 28800 28860 28920 28980 29040 29100 29160 29220 29280 29340 29400 29460 29520 29580 29640 29700 29760 29820 29880 29940 30000 30060 30120 30180 30240 30300 30360 30420 30480 30540 30600 30660 30720 30780 30840 30900 30960 31020 31080 31140 31200 31260 31320 201142028201142028 taaagaaaga gttattcgtt gtattgataa ctggtatgac cttgcatacc caccatcaga acaggagaaa gcaaaaaaag aagcaattga gaagttaata aagtaaagat aaagatggta tcaggttgtg gggcaacctg ataccgtgac acctgattct attgcgagga ataaaatgtc ggaacgcata ttatcagcaa taaatgacgt tgaaaagggt gggcgtccgg ttttcccttt gatgccattc catgtctttc ctgagtatat ggcattactc agaaaagcac tggaaaaaaa gacacaaaag agaacagata aataaaccgg aggctttatg acaaaacatg attttgtgtc ctttgtcagt ggagaattgc ggcaaggcgc tgtacgcttt tctcttgctt ttaacagcaa aggagaaatt gttctgcact ggactaataa agcaggaata cgagtatggc ggatattaag tggtaatcgt ggcaaaaaac caagcaaagc taaccttgaa agaatgagta acttccggcg ctggcttttt gatgcccgtc agggcatgga aggctacact cagcaacctg aacagagcaa ccttagctga atagccgaag ccctcccgta tttcgggagg tgctttctga tatattgcgc gggactgcgc ttgcgcttcc tgccagaccg ttcgcggagc gaacctgacg gcactgtcct taccgcattg atcgggtttc cggtcgcaga cgtacgtatg ttcagccatg acaacatcag cctttttgca tttttgttga tgctcaagca caatttatga gcggtgccag attcactgcg atgcaggata gggcaaacgc cgcaaaatga cgtctctgac gccattccgc aacgtttgcc cggtgaccta cagtcggtta ttgtcggaga attttatgaa aaaagttcaa ttcagaattg atgaaaatca gcataatgat ttgctggatt gtcttaaaac tctttatcca gatgaaccag ctttaacagt agctaaaggc atgaaacttt tagcaaatgc tttattaaaa agtaaagctg gcagtaagga cataaatacg ttttttgata ataatgattt tatcaaaaca acgatgtact taacaggtaa acaaagggct gatattgaaa gagctgctaa tcgtcacgga tggacgttat cacgagaatg tcgttaccgc atacagacga cacttgaaaa tgaactggat ttctttgacc aggaactgct gatgatgaat cgttgccgta attcaattga taagatcggt cgtaatttcc attatatcat tgttaatgat cagaccaggg ttcttgataa agatggtttc tatcaggatg cggagcgtct tacaacagaa atttttaatc ttaagaatca gtttgagaat tacattatgt tatgtaaagg gagaactgtt tcaaataaag tggagatgta attatgggcg tttacgttga taaagaatat agaaaatggt cgtgttaaac gaaagtcatc aaattctgga ggctaaagag cacatgatag cgtaagtcag ctttcgctca caaagtcaaa aatggtggaa agaactatag ccgcaatgtt caggaacgta tcaaccgcaa gggtgccagt aaggaggttg ttgtcaaaat atctggaggt gctgttactc gtcaggggat tcggaacagt attgattata tgagccgtga gtcagagcta ccagtgatga gtgaaagcgg tcgggtatgg atgggtgccg atcgtgctaa tgatcctcag catgtgatga atgataaagg taaagaaaat aaaaaaatca cacagaatat tgtcttctcg cctccagttt tagcgaaagt agtctgtcag aaagcctgaa gatttgttgg cattttataa agctaaagac tcttaacaag gaaaacgatg cagaaaaaat atcctaatca ccgttttgtt cttggatacc actgtgacaa gaaagaacat cctcacgttc atgttgtttt tcgtatccga gataatgacg gtaaacgcgc tgatatcagg aaaaaagatt tacgggaaat tcgtacaggt ttttgtgaag agttgaagtt aaaaggttat gacgttaaag cgacccataa gcaacagcat ggacttaatc agtctgttaa agatgcacat aatacagcac caaaaagaca gaaaggtgtt tatgaggttg ttgatattgg ctatgaccat tatcagaacg ataaaacaaa gtctaagcaa ggggttgaga aaacatactg gggggctgat tttggggact tatgttcgcg ggaaagtgtt aaagcaggtg atcttgtcag gctgaagaaa cttggtcaga aagaagtaaa aatcccggcg ctcgataaaa acggtgttca gcatggctgg aaaacggttc acagaaatga gtggcagtta gaaaatctgg gggttaaggg cgtagacaga acaccttcag ccagcaaaga gctggtactt aacagccctg atatgctgct gaagcaacaa cagcgaatgg cgcagtttac gcagcagaaa gcatccacgt tacagtcaga acagaagctg aaaacaggga ttaagttttt gggcttataa gatttaatgt attgattatg cgagaaaaat atttatttta gttcgcttgc tcatcttttc accttaaaa a cagatcgaaa ttctgcgttt tagctatcat tcgatctatc gatctaaaaa acagatctgc aaaagatgaa tattctgtgt ttcattgcat acatggaata aatgaaagta aaattgattt atcaagatcg aaagatctgt tttatgtgtt gaacttagca taagccactt gtagtataaa cttcgcgcaa tagtaaaaag tctctcttca taggggcgtg atttctcaca agcaaccacg gttggcttgt gttttgtcac atctgtatat cggggattga gtatgaaagg acagaaacag ttttacctgc ttcagtgttc tccgcatttt catcagtcgg ttctgttgca gttatcccgg agcggtatcg cttactacaa tccagttata cgcactttct ataagaggcg tgactgtagt gcatacaggg gacaaatcat gccaatgttt cccggttatg tattcgttct tctggatttt gaggttgtac atccatcctg ctttacccgg atgaaaaatg tttatggact ggtcagtttt ggtgaatatc ctgctgaagt tcctctgagt gtcataaatg aagtaaaaga gcaggagaag attttttcga tgaatttaag cctgatgaat aactcctgtc tggcaaaaat acttctcctt gcagacgctt caaagcgtgg acgagtcttt gccgactatg tgtgtaacag aaaatacgac aggttaaaaa atgacagcta taaaaggaaa gcggaaaccg caacgcaatg tactctacct tcctacagaa gttcgtgttg aagttgaaaa aattgcaatt gaaataagct tcaagagggg gcgacgtatc tctgattctg gttttgttca gtatcttatt 27780 27840 27900 27960 28020 28080 28140 28200 28260 28320 28380 28440 28500 28560 28620 28680 28740 28800 28860 28920 28980 29040 29100 29160 29220 29280 29340 29400 29460 29520 29580 29640 29700 29760 29820 29880 29940 30000 30060 30120 30180 30240 30300 30360 30420 30480 30540 30600 30660 30720 30780 30840 30900 30960 31020 31080 31140 31200 31260 31320 201142028

aaaaaataca aatctcaggc gatgaaagaa cttattcatg gggctgatat tcctgacgag 31380 taatattgtc ccaatgttct gaatgtggaa tatttatgtt atctacttct acttttcttg 31440 cgcttgccat gcaatgcgct gccagcgttc atcccgacac aacgcacgaa gtcgccaggg 31500 ttgaatcagg ttttaaccca tatgcgattg cggaaataat accaaaggtt aaacgtaaaa 31560 ctggtgataa aggcgtagta tcttactttc ctgaatcaaa ggaggcagca cttaagatcg 31620 ttaaaaacat tgaattacgg aatcatcgtt actctgtagg acttatgcaa ataacgagta 31680 ccaattttgc aaagttcggt acaacagcag agaaaatgtt tgatccatgc gaaaatctta 31740 aggtatcaga aaaaatactg gttgactgtt ataaacgagg tggcgactta gtgcgtgggc 31800 tgagttgcta ttattctggc aatcaagaaa caggagtaaa gccagaacct gaatttaata 31860 atacaagtta tgtacaacgt ataggattta gccctcctga taataaaaaa agttttattg 31920 ttccctctgt aaaggaaatg attaaaaagg agaataagac gactatcaca cctgaagaaa 31980 tcattatata tcctcaatac gccatgcgtg gcactgtatc aaatgaaaag gaaacaaaag 32040 atgttgaaat taaatctgaa taaacgttat ttaacgcttt ctctatttat ggctgcgttg 32100 atgctttgtg ttgcagaacc tgcttttgcg gatgatgtgt ctacgaagac aactggtttt 32160 ttacagaaaa taattgattt tttaacggat attcgtaaac ctgcaattac aattattgct 32220 cttgttattg ggtatattgc gatattttct cgtcaacata cctcatggat agtcccactt 32280 gttatcggaa ttatcatctt tattgttgca ccatatattc ctgactggct tgcgtaatta 32340 aaggatgggc gcactatgag tactcttttt aaaggtctta cgcgccctgc tttaataagg 32400 gggctgggcg ttccgctcta cccctttctt ggaatgtgca ttatttgtgt tctgcttggt 32460 gtctggattc atgaggctat gtatgccctt attcttcctg gctggtatgc catcaggcgt 32520 gtaacacagt ttgatgaacg cttttttgac cttctgtatc tgagaactct tgtcaaaggg 32580 catcctttat caaacaagcg attcagcgca gtccattatg cggggagcca gtacaatgaa 32640 gttgatattt caaaagtgga taactttatg aagctgaaag accagtcttc tgttgaagag 32700 ttaattccgt actcttcaca tatcactgat aatattatcg ttacaaaaaa ccgggatttg 32760 ctggcaacct ggcagataga cggtgcttac tttgagtgtg ttgattctga agatttgtca 32820 attctgacag atcagcttaa tacgcttata cgtagctttg aagggaaatt tgttacgctt 32880 tatcctcatc gtatcaggtg taaaaagggc gtcagaccag tatttaacag taaaattcct 32940 tttgtgaaca gagtaatgaa tgattattac gagtcattcc ctcagtctga atttttcgag 33000 aataaattat ttctgacgat ttgttttaaa ccttttacta cggaagataa agtaacacat 33060 ttcttttcac gcagtaaaaa acaaaaagat atctttaaag agcctgttaa tgaaatgaat 33120 gaaatttgcg acaggttgaa tacctatctg tcccgttttc attcccgacg tcttgggctt 33180 tatgaagatc atggggttgt ttattcagat cagttatctc tgttccagta tctgctttct 33240 ggtcgatggc aaaaggtcag ggttagcagt agtccgtttt atacatatct gggaggaaaa 33300 gacctgttct ttggtaatga tgccggacaa attaccgcgt cagaccatgc ccggtatttt 33360 cgttgcatag agattaagga ttattttcag gagacggatg ccggtattct ggatgctctg 33420 atgtatctcc ccgttgagta tgtcgtgaca tcgtccttta ctgcgatgga taagcagtca 33480 gcgattaagg cgctggatga tcagatcgat aagctggaaa tgacagatga tgctgccaaa 33540 tctttgctgg cagatctgaa agtcggactg gatatggttt ccagtggata tatttctttc 33600 ggaaaatcgc atcagaccct ggttgtcttt gcggattcac cggagcggct ggtgaaagac 33660 accaatatcg tgacttccac tctggaagat ttggggctga ttgtcactta ttcaacactg 33720 agtcttggcg cagcttattt tgctcagcta ccaggaaatt atacgcttcg ccctcgtctg 33780 agtaccctca gtagtcttaa ttttgccgaa atggaaagtt ttcataattt cttttcagga 33840 aaagaaaaag gaaatacctg gggggaaaaa ctgattactc ttcgggggtc aggtaatgat 33900 atctaccatc tgaattacca tatgacgact gaacatcaga atttcttcgg taagaacccg 33960 acgctggggc ataccgaaat tctcggtacg tctaacgtgg gtaaaaccgt attactgatg 34020 acaaaagcat ttgccgccca gcagttcggt acgccggaat cattccctgc aaacagaaaa 34080 ctgaaaaaac tgaccacggt tttttttgat aaagaccggg caggtgaagt cggtatacgg 34140 gcaatggggg gatcttatta ccgggtgaag gagggagagc cgacaggctg gaatcccgcc 34200 gcactgccgc caacaaagcg taatatcgct tttatgaagg acctggtgag gctgctttgt 34260 actctcaaca gtgagccgct cgatgattac cagaacagcc tgatttcaga tgcggttgaa 34320 cgtcttatgc aacggtcaga tcgctcttat cctgtcagta aactacggcc tcttatccag 34380 gagccggatg atactgaaac caaacgtcat ggacttaaag cccgtcttaa gccgtggacg 34440 cagggggaag agtttggctg ggtgttcgac aatcgggaag acacgtttga tgtcgataac 34500 ctggatgttt tcggtattga tggaacggag ttcctggata ataaggtgct ggccagtgct 34560 gcttcattct atctcatcta tcgggtcacc atgctggccg atggtcgcag gcttcttatc 34620 tacatggatg agttctggca atggatcaat aacgaagcgt tcagggactt tgtttacaac 34680 aagctgaaaa ccggacgtaa actcgatatg gtgcttgtcg tagccacaca gtcaccggat 34740 gaactgatta aatcacccat tgcggcagcg gttcgtgagc aatgcgccac tcatatctat 34800 ctggcaaacc cgaaagccaa acgtagtgaa tatgttgatg gtttgcaggt cagggagctt 34860 tattttgaca aaattaaagc tatcgatccg ctgtcccgcc agttccttgt tgttaagaac 34920 10aaaaaataca aatctcaggc gatgaaagaa cttattcatg gggctgatat tcctgacgag 31380 taatattgtc ccaatgttct gaatgtggaa tatttatgtt atctacttct acttttcttg 31440 cgcttgccat gcaatgcgct gccagcgttc atcccgacac aacgcacgaa gtcgccaggg 31500 ttgaatcagg ttttaaccca tatgcgattg cggaaataat accaaaggtt aaacgtaaaa 31560 ctggtgataa aggcgtagta tcttactttc ctgaatcaaa ggaggcagca cttaagatcg 31620 ttaaaaacat tgaattacgg aatcatcgtt actctgtagg acttatgcaa ataacgagta 31680 ccaattttgc aaagttcggt acaacagcag agaaaatgtt tgatccatgc gaaaatctta 31740 aggtatcaga aaaaatactg gttgactgtt ataaacgagg tggcgactta gtgcgtgggc 31800 tgagttgcta ttattctggc aatcaagaaa caggagtaaa gccagaacct gaatttaata 31860 atacaagtta tgtacaacgt ataggattta gccctcctga taataaaaaa agttttattg 31920 ttccctctgt aaaggaaatg attaaaaagg agaataagac gactatcaca cctgaagaaa 31980 tcattatata tcctcaatac gccatgcgtg gcactgtatc aaatgaaaag gaaacaaaag 32040 atgttgaaat taaatctgaa taaacgttat ttaacgcttt ctctatttat ggctgcgttg 32100 atgctttgtg ttgcagaacc tgcttttgcg gatgatgtgt ctacgaagac aactggttt t 32160 ttacagaaaa taattgattt tttaacggat attcgtaaac ctgcaattac aattattgct 32220 cttgttattg ggtatattgc gatattttct cgtcaacata cctcatggat agtcccactt 32280 gttatcggaa ttatcatctt tattgttgca ccatatattc ctgactggct tgcgtaatta 32340 aaggatgggc gcactatgag tactcttttt aaaggtctta cgcgccctgc tttaataagg 32400 gggctgggcg ttccgctcta cccctttctt ggaatgtgca ttatttgtgt tctgcttggt 32460 gtctggattc atgaggctat gtatgccctt attcttcctg gctggtatgc catcaggcgt 32520 gtaacacagt ttgatgaacg cttttttgac cttctgtatc tgagaactct tgtcaaaggg 32580 catcctttat caaacaagcg attcagcgca gtccattatg cggggagcca gtacaatgaa 32640 gttgatattt caaaagtgga taactttatg aagctgaaag accagtcttc tgttgaagag 32700 ttaattccgt actcttcaca tatcactgat aatattatcg ttacaaaaaa ccgggatttg 32760 ctggcaacct ggcagataga cggtgcttac tttgagtgtg ttgattctga agatttgtca 32820 attctgacag atcagcttaa tacgcttata cgtagctttg aagggaaatt tgttacgctt 32880 tatcctcatc gtatcaggtg taaaaagggc gtcagaccag tatttaacag taaaattcct 32940 tttgtgaaca gagtaatgaa tgattattac gagtcattcc ctcagtctga a tttttcgag 33000 aataaattat ttctgacgat ccttttacta cggaagataa agtaacacat 33060 ttcttttcac gcagtaaaaa acaaaaagat atctttaaag agcctgttaa tgaaatgaat 33120 gaaatttgcg acaggttgaa tacctatctg tcccgttttc attcccgacg tcttgggctt 33180 tatgaagatc atggggttgt ttattcagat cagttatctc tgttccagta tctgctttct 33240 ggtcgatggc aaaaggtcag ggttagcagt agtccgtttt atacatatct gggaggaaaa 33300 gacctgttct ttggtaatga tgccggacaa attaccgcgt cagaccatgc ccggtatttt 33360 cgttgcatag agattaagga ttattttcag gagacggatg ccggtattct ggatgctctg ttgttttaaa 33420 atgtatctcc ccgttgagta tgtcgtgaca tcgtccttta ctgcgatgga taagcagtca 33480 gcgattaagg cgctggatga tcagatcgat aagctggaaa tgacagatga tgctgccaaa 33540 tctttgctgg cagatctgaa agtcggactg gatatggttt ccagtggata tatttctttc 33600 ggaaaatcgc atcagaccct ggttgtcttt gcggattcac cggagcggct ggtgaaagac 33660 accaatatcg tgacttccac tctggaagat ttggggctga ttgtcactta ttcaacactg 33720 agtcttggcg cagcttattt tgctcagcta ccaggaaatt atacgcttcg ccctcgtctg 33780 agtaccctca gtagtcttaa ttttgccgaa atggaaagtt ttca taattt cttttcagga 33840 aaagaaaaag gaaatacctg gggggaaaaa ctgattactc ttcgggggtc aggtaatgat 33900 atctaccatc tgaattacca tatgacgact gaacatcaga atttcttcgg taagaacccg 33960 acgctggggc ataccgaaat tctcggtacg tctaacgtgg gtaaaaccgt attactgatg 34020 acaaaagcat ttgccgccca gcagttcggt acgccggaat cattccctgc aaacagaaaa 34080 ctgaaaaaac tgaccacggt tttttttgat aaagaccggg caggtgaagt cggtatacgg 34140 gcaatggggg gatcttatta ccgggtgaag gagggagagc cgacaggctg gaatcccgcc 34200 gcactgccgc caacaaagcg taatatcgct tttatgaagg acctggtgag gctgctttgt 34260 actctcaaca gtgagccgct cgatgattac cagaacagcc tgatttcaga tgcggttgaa 34320 cgtcttatgc aacggtcaga tcgctcttat cctgtcagta aactacggcc tcttatccag 34380 gagccggatg atactgaaac caaacgtcat ggacttaaag cccgtcttaa gccgtggacg 34440 cagggggaag agtttggctg ggtgttcgac aatcgggaag acacgtttga tgtcgataac 34500 ctggatgttt tcggtattga tggaacggag ttcctggata ataaggtgct ggccagtgct 34560 gcttcattct atctcatcta tcgggtcacc atgctggccg atggtcgcag gcttcttatc 34620 tacatggatg agttctggca atggatcaat aacgaag cgt tcagggactt tgtttacaac 34680 aagctgaaaa ccggacgtaa actcgatatg gtgcttgtcg tagccacaca gtcaccggat 34740 gaactgatta aatcacccat tgcggcagcg gttcgtgagc aatgcgccac tcatatctat 34800 ctggcaaacc cgaaagccaa acgtagtgaa tatgttgatg gtttgcaggt cagggagctt 34860 tattttgaca aaattaaagc tatcgatccg ctgtcccgcc agttccttgt tgttaagaac 34920 10

201142028 ccacagagga aaggtgaaag tgatgatttt gctgcttttg ccagactgga gctgggaaaa gcagcgtatt acttaccggt tctcagtgca tcaaaacccc agttagaact gttcgatgaa atctggaaag aaggaatgaa gccggaagag tggcttgata cctatctgga acaggcgaac ctgatttgag gaatcaccaa tgaaaaagca aattatggcg gcattcgtcg cttcactgat tgttatttcc ggcgctcagg cagggatacc tgttgccatc gacgccaacc ctgaatgggc gattgaagcc ggacgatgga cagaacgcct taagcaatgg gcggaaacgg taaaacatta cgaaaatcag ataaatgcgt acaaacagga gctgctgtca aaaacgggta tccgtgatgt gcagggactg gtgcagtccg cacagtcagt gagtcaggaa ctgatgcaga tttatgatca ggggaatgct tttattgacg attacattaa aaatcctgaa ggggcgttat cggaacaggc caaatcgtta ttgtcagatt acaaagtaac ggatacctgc cagaacctgg gatattccgg cgacctggta cggggatgtg aagcgacgtt cctttctcaa ctggcaagcg tggaatacgg taacaagctg gagagcaagc ttcgtcagga caaccagacg atgaaagacc ttattgatca agtcaaaaat gcgaaggata cgaaggccac gcaggatgca acaaacgctg ttgcacttga acaactgaag ttcgagaagc tcaaatttca gtatcaaatg tatcgcgata agcagcgaga tcttgcagaa tataaagaga agatggctca ggcagctttc agaaaacagc aacgtgaagc cgtgccacct tcttacagaa aagcttatat ggcaatgaaa tcatatgagg atgattaaat gaaaagtatc gctactgctg gccttgtcat tatatctgtt tttagtcttt ctggatgttt tgaagaaaca aaatcggttg attggtggct tgcacatccg aaggagacat acaaaaaatt tgaagagtgt cagaagtctg gtagcgattc tgataattgt aagaacgtta agcgagcaca tttgtctttt gaacggagaa aggcggttgg tttaccaata aattgagggt gatgtatggg gattgtcact ggtataccgg atggtactga tttatcaggt aatgtaacct ctcccgcaaa tgcgggaggt gtttctggat ttgattctga tttttttcag acaactcatg aagttatatt taatattctc aacaagagta tatctggaaa attaagtgaa tattcagatg tggcttatac tcttggtaaa tatggagttt ctctgtatgt tttatggtat gcttttactg tattagcaag gaaacaacag acacctgtac ctgattttat ctggaatatc tgtaggtttt acataatatt gctttttgtt aagaatacag ggggaatact tacatcagca acagatgcta ttgatggatt gaaaaataca ttggcagggg gagatccgtg ggtatggatg gatcagttat gggtgaaggt tatacaagtt gcaactctta tttttgataa agatacatct actgtgcctg ttgccggtgg gattggtgct ttattaactt atgtcggtgg tgttttggca ttattgcttt gttctatagt atttgcatct gctgaattaa cattactatt actttctgtc actgcgccaa tatttatcat gtgtctgatg tttggtttac ttcggcaaat gtttaatagc tggctacagc ttaattttag ctcgttactg gtttttctat ttgcagcatt agcactaaga gctgggacat ggcaattaaa catggcatta agtacgtcta ttgctacagc atcagaaaac aatcttcttc aaacgggagt aacttcatta gctgctggca ttttcatggc ctggattatc tggcaggcga aaagttatgc ttcacagatt gcaggtgtgg gtgttgaagg tgccatgcag ggcgcagccg ctatggggat tggtgctggc gttttcggtg catcccgtat ggcgcgtggc gcacttggta tgggcagaaa tgccggtatt ggtgcatgga aaggtctgcg tcgtcaggaa gacgggtttg gtcagtctcc gggaataacg ggtaagaccg ctaacctggc cggacagggc gttaatattg gtgccaaaaa gcttcgtcag gcagctattg agagagcaaa gaaaatgtat ggtgggtaat ttaattatga gcgcctttta atgttctttt cataattgtc attgtcgggc ctggcgcgct ctattatctt ttctgttcac actgagtctt ctgtaaatat taatctgtta tacaggtgtt catattatga aactacttat tgtggctttc gtgacgcttt gtctcgctgg ttgtcaggcg tcacataaac taccgcccgt ttccgggaaa agcgaacctg ttaattctgc tgaggtaatg caaaatggaa tttaaacttc ccggatttaa aaataaaaaa gacgttactg actcatcagt ttcatttgaa gaaaaaaaca ttgcactaca ggagagaatg aatcgtattt ataaattcgg tggtatcgga ggcatgttaa ttggtgggct gtctttactt gcattaaatg cagcattacc actgaaaaca acagttgttg atgcctacct tatcgataag gttacaggtg tggctgaacg tctgacttct gttaaaaaag aaaatctttc tgaaaacgaa gccattgccc gatattttat cacccagtat ataaaacatc gtgaaggtta taattttttc agtctccagc atgattatga ttatgtaatg gcttacagcg cggagaatgt cgcggcagat tataacgcat tatttaacag tgaacaggca ccaaaacttg tttataacaa agcagaaaaa acggcaatgg ttcaggataa tccatctgtc ataatttcac cttcgtcacg ggcagatgat aaagatatcg gtgcgtatat tcgttttcgt ctgaccatca gggatgttgc taccggacaa acccgccagg agttctggaa tgttcgcctg acttatcgta tcgaaccgca ggttgaaatg gtgtcagggg aacgtaataa caatcctctt aaattcgttg taacaagcta cgttcgcgat aaagaagcca gaggttaata atatgaaaat gaataaagga gcgttaatta tggcgcttct gatggcggcg cacgtctgtc atgcagctgt tcttccttca ggcagtcgct ttgacccacg caatcagata gtcagttata accccaataa taccaccata attaacagtg ccgttggata caccaccaca ctggtatttg atgaagatga aacagttatc agtgccagaa ctggttttcc gcagggatgg gcggttaata aagaagataa cctggtatac ctggaagttc gtcctgttaa acagactgtt cagaaaaata atatggatga 34980 35040 35100 35160 35220 35280 35340 35400 35460 35520 35580 35640 35700 35760 35820 35880 35940 36000 36060 36120 36180 36240 36300 36360 36420 36480 36540 36600 36660 36720 36780 36840 36900 36960 37020 37080 37140 37200 37260 37320 37380 37440 37500 37560 37620 37680 37740 37800 37860 37920 37980 38040 38100 38160 38220 38280 38340 38400 38460 38520 11 201142028201142028 ccacagagga aaggtgaaag tgatgatttt gctgcttttg ccagactgga gctgggaaaa gcagcgtatt acttaccggt tctcagtgca tcaaaacccc agttagaact gttcgatgaa atctggaaag aaggaatgaa gccggaagag tggcttgata cctatctgga acaggcgaac ctgatttgag gaatcaccaa tgaaaaagca aattatggcg gcattcgtcg cttcactgat tgttatttcc ggcgctcagg cagggatacc tgttgccatc gacgccaacc ctgaatgggc gattgaagcc ggacgatgga cagaacgcct taagcaatgg gcggaaacgg taaaacatta cgaaaatcag ataaatgcgt acaaacagga gctgctgtca aaaacgggta tccgtgatgt gcagggactg gtgcagtccg cacagtcagt gagtcaggaa ctgatgcaga tttatgatca ggggaatgct tttattgacg attacattaa aaatcctgaa ggggcgttat cggaacaggc caaatcgtta ttgtcagatt acaaagtaac ggatacctgc cagaacctgg gatattccgg cgacctggta cggggatgtg aagcgacgtt cctttctcaa ctggcaagcg tggaatacgg taacaagctg gagagcaagc ttcgtcagga caaccagacg atgaaagacc ttattgatca agtcaaaaat gcgaaggata cgaaggccac gcaggatgca acaaacgctg ttgcacttga acaactgaag ttcgagaagc tcaaatttca gtatcaaatg tatcgcgata agcagcgaga tcttgcagaa tataaagaga agatggctca ggcagctttc agaaaacagc aacgtgaagc cgtgccacct tcttacagaa aagcttatat ggcaatgaaa tcatatgagg atgattaaat gaaaagtatc gctactgctg gccttgtcat tatatctgtt tttagtcttt ctggatgttt tgaagaaaca aaatcggttg attggtggct tgcacatccg aaggagacat acaaaaaatt tgaagagtgt cagaagtctg gtagcgattc tgataattgt aagaacgtta agcgagcaca tttgtctttt gaacggagaa aggcggttgg tttaccaata aattgagggt gatgtatggg gattgtcact ggtataccgg atggtactga tttatcaggt aatgtaacct ctcccgcaaa tgcgggaggt gtttctggat ttgattctga tttttttcag acaactcatg aagttatatt taatattctc aacaagagta tatctggaaa attaagtgaa tattcagatg tggcttatac tcttggtaaa tatggagttt ctctgtatgt tttatggtat gcttttactg tattagcaag gaaacaacag acacctgtac ctgattttat ctggaatatc tgtaggtttt acataatatt gctttttgtt aagaatacag ggggaatact tacatcagca acagatgcta ttgatggatt gaaaaataca ttggcagggg gagatccgtg ggtatggatg gatcagttat gggtgaaggt tatacaagtt gcaactctta tttttgataa agatacatct actgtgcctg ttgccggtgg gattggtgct ttattaactt atgtcggtgg tgttttggca ttattgcttt gttctatagt atttgcatct gctgaattaa cattactatt actttctgtc actgcgccaa tatttatcat gtgtctgatg tttggtttac ttcggcaaat gtttaatagc tggctacagc ttaattttag ctcgttactg gtttttctat ttgcagcatt agcactaaga gctgggacat ggcaattaaa catggcatta agtacgtcta ttgctacagc atcagaaaac aatcttcttc aaacgggagt aacttcatta gctgctggca ttttcatggc ctggattatc tggcaggcga aaagttatgc ttcacagatt gcaggtgtgg gtgttgaagg tgccatgcag ggcgcagccg ctatggggat tggtgctggc gttttcggtg catcccgtat ggcgcgtggc gcacttggta tgggcagaaa tgccggtatt ggtgcatgga aaggtctgcg tcgtcaggaa gacgggtttg gtcagtctcc gggaataacg ggtaagaccg ctaacctggc cggacagggc gttaatattg gtgccaaaaa gcttcgtcag gcagctattg agagagcaaa gaaaatgtat ggtgggtaat ttaattatga gcgcctttta atgttctttt cataattgtc attgtcgggc ctggcgcgct ctattatctt ttctgttcac actgagtctt ctgtaaatat taatctgtta tacaggtgtt catattatga aactacttat tgtggctttc gtgacgcttt gtctcgctgg ttgtcaggcg tcacataaac taccgcccgt ttccgggaaa agcgaacctg ttaattctgc tgaggtaatg caaaatggaa tttaaacttc ccggatttaa aaataaaaaa gacgttactg actcatcagt ttcatttgaa gaaaaaaaca ttgcactaca ggagagaatg aatcgtattt ataaattcgg tggtatcgga ggcatgttaa ttggtgggc t gtctttactt gcattaaatg cagcattacc actgaaaaca acagttgttg atgcctacct tatcgataag gttacaggtg tggctgaacg tctgacttct gttaaaaaag aaaatctttc tgaaaacgaa gccattgccc gatattttat cacccagtat ataaaacatc gtgaaggtta taattttttc agtctccagc atgattatga ttatgtaatg gcttacagcg cggagaatgt cgcggcagat tataacgcat tatttaacag tgaacaggca ccaaaacttg tttataacaa agcagaaaaa acggcaatgg ttcaggataa tccatctgtc ataatttcac cttcgtcacg ggcagatgat aaagatatcg gtgcgtatat tcgttttcgt ctgaccatca gggatgttgc taccggacaa acccgccagg agttctggaa tgttcgcctg acttatcgta tcgaaccgca ggttgaaatg gtgtcagggg aacgtaataa caatcctctt aaattcgttg taacaagcta cgttcgcgat aaagaagcca gaggttaata atatgaaaat gaataaagga gcgttaatta tggcgcttct gatggcggcg cacgtctgtc atgcagctgt tcttccttca ggcagtcgct ttgacccacg caatcagata gtcagttata accccaataa taccaccata attaacagtg ccgttggata caccaccaca ctggtatttg atgaagatga aacagttatc agtgccagaa ctggttttcc gcagggatgg gcggttaata aagaagataa cctggtatac ctggaagttc gtcctgttaa acagactgtt cagaaaaata atatggatga 34980 35040 35100 35160 35220 35280 35340 35400 35460 35520 35580 35640 35700 35760 35820 35880 35940 36000 36060 36120 36180 36240 36300 36360 36420 36480 36540 36600 36660 36720 36780 36840 36900 36960 37020 37080 37140 37200 37260 37320 37380 37440 37500 37560 37620 37680 37740 37800 37860 37920 37980 38040 38100 38160 38220 38280 38340 38400 38460 38520 11 201142028

atiacggtaat acctcttctg aatccgtcag tgttgctctt gacccggaaa atgagcttga 38580 acgctggcga acgaatttgt ttgttcgcac cacgaagcgt aattacagca tggagctgaa 38640 cgcccggacg ttccggcagc cggagaaaat tgcgtttgtg gtgaattacc agtatccgca 38700 ggaacgccgg aaggaacagg ccgaaattga gaagaaacgc acagaggctc ttgccagacg 38760 ccaggaggag caggcaatca accgttccct ggaaaatgcg aaatcgcccc gtaactggca 38820 gtactggaag cgggttgctg aaggcagcca ggatatcagc cctgattatg catatgacga 38880 tggccgttat acctggttcg gcttcagtcc gttaaagaaa attcccagcg tctttgtgat 38940 gaacggtatg caggagactc ttaccaatcc tgtgattaaa cagagcggga gttttacggc 39000 tgttggcgta ccggttgata agcgttttgt tttacgtctt ggtgagcagg tggtggggat 39060 tgagaaccag ggcttcggaa aagtacgttt accagccgga gatacggtat ccccggatgt 39120 taagaaagag gtgatccagt gactgaacag gaaaataaaa tcccgactgc aaccgaaatt 39180 gaacagcagc tacgggaacg cagacagaaa gaactggaac aggccgggaa gactccggaa 39240 gaagagcctg gcaagccagc attgcagctt ggtattgaaa aacttaaaaa gtcacgtaaa 39300 gggatgatta tcctcgtcgt gggttttctt ctgcttgctg ccggtgtttc tgtttattat 39360 atcccgtcca ttatccgttc tgtgtcgtca ggggatgaga aacccgcaag tcagccgctt 39420 gcaaccggaa cggctaaacg tcagaccgga ctgagcgaag atatcgatcc ttttaatacc 39480 gcacagaaaa aaacagagaa accagaggaa gaaaaagtca tttcttctga aaagactgaa 39540 ccgccggaaa ataaacagca gagcttcagc cgtgcacttg acgtttctct tgatggcagc 39600 cagacaggaa acagcagcag ttcagcggga acgtcagttt cacatactgc ggccagtgag 39660 ccagaaagcg ataaaaagga tgaagcaaaa gcaaccgcac agactacaga atctgcgcca 39720 ctggcgaaaa taacgaaact tccatatgac ccaaatttgt ttatcccgga agggacatca 39780 attccctgtt cactggacag gcgttttgtt tctgacctgg cggggaaact ggaatgtacg 39840 gtcaacagcg atatatacag cgccagcggt aatgtaaaac ttatcgaaag aggaaccgcc 39900 gcaaaactga tgtataaggc cgggtcttta aatcatggac aagggcgtgt gtttgttatg 39960 gcttacaagc tacgtacccg cagtaagcct tttattgata ttcccctggt tgactcacag 40020 gcggctggcg cgttaggcga agccggtgct tctgggtgga ttgacactca tttcagtgaa 40080 cgttttcttg gtgcaatgat ggtcgggatg ataccggatt taagtcaggc cgccagtggt 40140 attgcacaga acaacaggga cagccagacc gactatacgg caaacagtcg ccaggctttt 40200 gctgatatag cacgcgaagc attttctaat agtgtgaata ttccgccaac gctttataaa 40260 aatcagggcg aaattattac tctgattgtc ggtcaggatc tggatttttc aggcatttat 40320 aaactgaaaa tgaaaggggg ttaatgtgaa taacgaaaac agacatctga tttatgatgt 40380 ggtcaacgat tatttttatc actggctgaa tgagattgag ggtgtcacgg aaattgctgt 40440 taaccgacca ggagaaatat ttataaaggt caggggaaag tggcaatggt atgaacaaaa 40500 gatgagttac agtgattgtc tttcttttgc atccacactg gccgattttc atgacggcgg 40560 ttctgtgact cctgaatatc ccctgcgctc tgccacgctt ccgggtggag aacgtgttca 40620 ggttgtgatc ccaccggcaa ctgaaaaaga cactgtttct ataacaatcc gtaagccgtc 40680 aggtattttt atcagtcatg acaaatttat aaaacaggga ttttattcac gcgtcagtgg 40740 tttaagtggt gactcggtta tggaagataa tatttctgct ttaatcactt ccggatattt 40800 tgatcgggtt atacctgaat cactgcgtca ggggaaaacg atagttttct gtggagggac 40860 gggttcaggt aaaactacct ttgcaaatgc ctgtctggaa tatataccgc atcatctgcg 40920 gtgtatttct attgaagata ctgatgaggc aaaattcaga ttccataaaa accatgtaaa 40980 actttactat ccggcagagg gtgagagtaa ggttattacc tcagcgagtc ttctgcgttc 41040 ctgttttcgt atgaatccgg acaggattct gatgacagaa atcagggggg ctgaggcatg 41100 ggattttctg aaagcatcga gttcaggcca tgcaggaaac attaccaccg ttcacgaaag 41160 tagtcctgaa tatgctgtgc ttgggattgt tcagcgatgt tatatgaatc ctgaatgtca 41220 gaatctacca ttcaatgtca ttttaagacg tgtactgagt aatattgata ttatcatgag 41280 tattaaatac cttgatgatg aagattttcg tttcgcttcc ggtatttatt acaaacaact 41340 tcattttgat gactatttca gaaaactgaa ggagtgatta tgtctttaaa actccccgat 41400 aaaggccagt gggtttttat cggtctggtt atgtgtctcg tgacatatta tactggttct 41460 gttgctgttt acttcctgaa cggaaaaacg ccgctttata tatggaaaaa ttttgattcc 41520 atgctcctgt ggcgaataat aacagagagt aatatacgga cagatatcag gttaaccgct 41580 atcccctctc ttttatcagg tatggtttcg tctctcatcg tgcctgtttt tattatctgg 41640 caactgaata aaacggctgt tgctctttat ggtgacgcga agtttgccag tgataatgat 41700 ttaaggaaat cgaaacttct gaaatgggag aaagaaaacg atactgatat tctcgtcgga 41760 gcatataaag gtaaatacct gtggtatacc gcaccagatt ttgtatcact tggcgcagga 41820 acccgcgcag gtaaaggtgc cgccattggt atccGtaatc ttctggtcag aaaacactct 41880 ctgattgcgt tagatccaaa acaggaattg tggaaaatca ccagtaaggt gcgtgaaata 41940 ctgctgggta ataaagttta tctgctcgac cctttcaaca gtaaaacaca ccagtttaat 42000 ccccttttct atattgattt aaaagcggag agtggggcta aggatctgct taaactgatt 42060 gaaattctgt ttccgtctta tggcatgaca ggggcagaag cgcactttaa taatcttgcg 42120 12atiacggtaat acctcttctg aatccgtcag tgttgctctt gacccggaaa atgagcttga 38580 acgctggcga acgaatttgt ttgttcgcac cacgaagcgt aattacagca tggagctgaa 38640 cgcccggacg ttccggcagc cggagaaaat tgcgtttgtg gtgaattacc agtatccgca 38700 ggaacgccgg aaggaacagg ccgaaattga gaagaaacgc acagaggctc ttgccagacg 38760 ccaggaggag caggcaatca accgttccct ggaaaatgcg aaatcgcccc gtaactggca 38820 gtactggaag cgggttgctg aaggcagcca ggatatcagc cctgattatg catatgacga 38880 tggccgttat acctggttcg gcttcagtcc gttaaagaaa attcccagcg tctttgtgat 38940 gaacggtatg caggagactc ttaccaatcc tgtgattaaa cagagcggga gttttacggc 39000 tgttggcgta ccggttgata agcgttttgt tttacgtctt ggtgagcagg tggtggggat 39060 tgagaaccag ggcttcggaa aagtacgttt accagccgga gatacggtat ccccggatgt 39120 taagaaagag gtgatccagt gactgaacag gaaaataaaa tcccgactgc aaccgaaatt 39180 gaacagcagc tacgggaacg cagacagaaa gaactggaac aggccgggaa gactccggaa 39240 gaagagcctg gcaagccagc attgcagctt ggtattgaaa aacttaaaaa gtcacgtaaa 39300 gggatgatta tcctcgtcgt gggttttctt ctgcttgctg ccggtgtttc tgtttatt at 39360 atcccgtcca ttatccgttc tgtgtcgtca ggggatgaga aacccgcaag tcagccgctt 39420 gcaaccggaa cggctaaacg tcagaccgga ctgagcgaag atatcgatcc ttttaatacc 39480 gcacagaaaa aaacagagaa accagaggaa gaaaaagtca tttcttctga aaagactgaa 39540 ccgccggaaa ataaacagca gagcttcagc cgtgcacttg acgtttctct tgatggcagc 39600 cagacaggaa acagcagcag ttcagcggga acgtcagttt cacatactgc ggccagtgag 39660 ccagaaagcg ataaaaagga tgaagcaaaa gcaaccgcac agactacaga atctgcgcca 39720 ctggcgaaaa taacgaaact tccatatgac ccaaatttgt ttatcccgga agggacatca 39780 attccctgtt cactggacag gcgttttgtt tctgacctgg cggggaaact ggaatgtacg 39840 gtcaacagcg atatatacag cgccagcggt aatgtaaaac ttatcgaaag aggaaccgcc 39900 gcaaaactga tgtataaggc cgggtcttta aatcatggac aagggcgtgt gtttgttatg 39960 gcttacaagc tacgtacccg cagtaagcct tttattgata ttcccctggt tgactcacag 40020 gcggctggcg cgttaggcga agccggtgct tctgggtgga ttgacactca tttcagtgaa 40080 cgttttcttg gtgcaatgat ggtcgggatg ataccggatt taagtcaggc cgccagtggt 40140 attgcacaga acaacaggga cagccagacc gactatacgg caaacagtcg ccaggctttt 40200 gctgatatag cacgcgaagc attttctaat agtgtgaata ttccgccaac gctttataaa 40260 aatcagggcg aaattattac tctgattgtc ggtcaggatc tggatttttc aggcatttat 40320 aaactgaaaa tgaaaggggg ttaatgtgaa taacgaaaac agacatctga tttatgatgt 40380 ggtcaacgat tatttttatc actggctgaa tgagattgag ggtgtcacgg aaattgctgt 40440 taaccgacca ggagaaatat ttataaaggt caggggaaag tggcaatggt atgaacaaaa 40500 gatgagttac agtgattgtc tttcttttgc atccacactg gccgattttc atgacggcgg 40560 ttctgtgact cctgaatatc ccctgcgctc tgccacgctt ccgggtggag aacgtgttca 40620 ggttgtgatc ccaccggcaa ctgaaaaaga cactgtttct ataacaatcc gtaagccgtc 40680 aggtattttt atcagtcatg acaaatttat aaaacaggga ttttattcac gcgtcagtgg 40740 tttaagtggt gactcggtta tggaagataa tatttctgct ttaatcactt ccggatattt 40800 tgatcgggtt atacctgaat cactgcgtca ggggaaaacg atagttttct gtggagggac 40860 gggttcaggt aaaactacct ttgcaaatgc ctgtctggaa tatataccgc atcatctgcg 40920 gtgtatttct attgaagata ctgatgaggc aaaattcaga ttccataaaa accatgtaaa 40980 actttactat ccggcagagg gtgagagtaa ggttattacc tca gcgagtc ttctgcgttc 41040 ctgttttcgt atgaatccgg acaggattct gatgacagaa atcagggggg ctgaggcatg 41100 ggattttctg aaagcatcga gttcaggcca tgcaggaaac attaccaccg ttcacgaaag 41160 tagtcctgaa tatgctgtgc ttgggattgt tcagcgatgt tatatgaatc ctgaatgtca 41220 gaatctacca ttcaatgtca ttttaagacg tgtactgagt aatattgata ttatcatgag 41280 tattaaatac cttgatgatg aagattttcg tttcgcttcc ggtatttatt acaaacaact 41340 tcattttgat gactatttca gaaaactgaa ggagtgatta tgtctttaaa actccccgat 41400 aaaggccagt gggtttttat cggtctggtt atgtgtctcg tgacatatta tactggttct 41460 gttgctgttt acttcctgaa cggaaaaacg ccgctttata tatggaaaaa ttttgattcc 41520 atgctcctgt ggcgaataat aacagagagt aatatacgga cagatatcag gttaaccgct 41580 atcccctctc ttttatcagg tatggtttcg tctctcatcg tgcctgtttt tattatctgg 41640 caactgaata aaacggctgt tgctctttat ggtgacgcga agtttgccag tgataatgat 41700 ttaaggaaat cgaaacttct gaaatgggag aaagaaaacg atactgatat tctcgtcgga 41760 gcatataaag gtaaatacct gtggtatacc gcaccagatt ttgtatcact tggcgcagga 41820 acccgcgcag gtaaaggtgc cgccattggt atccGt aatc ttctggtcag aaaacactct 41880 ctgattgcgt tagatccaaa acaggaattg tggaaaatca ccagtaaggt gcgtgaaata 41940 ctgctgggta ataaagttta tctgctcgac cctttcaaca gtaaaacaca ccagtttaat 42000 ccccttttct atattgattt aaaagcggag agtggggcta aggatctgct taaactgatt 42060 gaaattctgt ttccgtctta tggcatgaca ggggcagaag cgcactttaa taatcttgcg 42120 12

201142028 ggtcaatact ggacaggact ggctaagttg cttcatttct ttattaacta tgagccgtcc tggcttaatg agttcgggct taaacccgtt ttctcaatcg gttctgtcgt cgacttgtac agcaatattg accgggaact gatactcagt aagcgggaag aactggaggg aacaaacggg cttgatgaaa acgcgttgta tcatttgcgc gatgccctga ccaaaatcag ggaatatcac gaaacggaag atgaacagcg ttcaagcatt gatggttctt tccgtaagaa aatgagcctg ttttatctcc caaccgttcg taaatgtact gatggtaatg atttcgatct ccgtcagttg cgacgggaag atatcactgt ttatgtcggt gttaatgcgg aagatatatc actggcttac gattttctga acctgttttt caacttcgtt gttgaagtga cattgcgtga aaatcctgat tttgatccca ccctgaaaca tgactgcctg atgtttcttg atgagttccc ttcgattggt tatatgccaa ttattaaaaa gggatcaggg tatattgcag gttttaaact taaactgctg acaatttatc agaatatcag tcagctaaat gaaatctatg gtattgaggg agccaaaacg ctgatgagtg ctcatccctg ccgtattatc tatgctgtca gcgaagagga tgatgccgcg aagatatcag aaaaacttgg gtatattacc actacatcaa agagcacaag caagaaccgg ggacgatcaa cttcacaggg cgaatcagaa agtgaagccc gaagagcact ggtgcttcca caggaactgg gaacgctgga ctttaaagaa gagtttatca tcctgaaggg ggagaaccct gttaaagcag aaaaggcact ttattacctt gatccgtatt ttatggacag gttaatgaag gtcagtccta aactggcatc attgacgatg aaactaaata agacgaaaaa aatatttggt gtgaaagggc ttaaatatcc gtcaaaagaa aaaatgctct ccgtaggaga gctggagtct gaggttttgc tatgaaaaaa atacttatga tcagtattct tgtcctcaca gcctgttcct ctccacctga accgccacag gttgaatggg aaaaaaggcc tgaagttatg aatacacaaa taatgaactg gaaaccaaca tccggtgtta ttaaatcaaa taatataaat tcctcatgga gtaaggtgtt gcctgatttt aaaccagaaa accatcttta cgatgattct gttttttatg ccgttgccca ttctgaaaaa atagttgtaa ggacatcttc ttttgatagt tactggtcag cgaaagactg gctaagaaaa aatggtgcaa caggtgttat tgaatatcag ccactaaaaa gatggttgaa taatgactat gttgaaattt atctgtcaag aataaatatt cagaggttac tatgaaaagg atatgcaaag gtctaatcgt aatttttact gtgtcctgtt ttatagcgcc aacgtatgcc gctgatccct gtaaatccgt tttttgtctt tatggtaaag ctgtcggtag tagtggaggg agtgagtgca gcagtgcaga aaaggatttt tttaaaaatg ttgaaaagaa aaaggggaaa atccgctggg gtaaaacatt tgatctccgt aagaattttt taaatcagtg ttcaacggca gacccggctg caatctcact gatcatgagt aagtttggtc gcgtcagagg ttgattaaaa cctgataccc gcgccaacgg gtatcagggg actgcaagta tcacataccg cgccaacgaa atgtgatgat tagttacaat cactgaggtt agaaatgaaa gtaaccaaat ctattatatc tattttattg tgcttatctg ctggtagtgc ataagtggat gtaataaaaa atgctcgtac ctttttgaaa ggaacccttg atctgagcgt gcaggaagca gatgaaaggg aagaacttta taagaagaac ggggcgcaac ctgactattt aagttacctg gaggattaac acatgcaata tgcattattt gatgggatgg aacgaaagtt tttgctggat gctcttgaat ttggtgttct gaaggactgg aaagaaaatc cggtaaaaga acttcctgat attgatgaat ctgttcaccc cttccatgtc tgttatggtg gatatttatt aaaccctgat gtttcagatt tagatattag cagaaaaata aaagaccaga caggattctg gctggcagct attgatgata cccgtatgga ttgtcattca atagcttatt atgatattca caccctccct ttaatttcgt gtggtcatca gaagatagtt ccttttgcag cgttaataaa agctgatgaa tgcatcattt caaaaattgc ttcgtattct ggttttgccg taacagcctt tttgagaatt aaagaccaga atatcgcaac gaatatactt aaccgtgagg gaatttttgc ctttaatggc tgtgagcaca gattcagaca acctgtaagt gaagataact ggcaacaggc agtgtcagaa gaacgcgcta tccgttgtgc caaaagatta attcaatgta aaggataaca aaatgagact ttttatcgct gaaaaacccg cagtagcaaa tgatattgtt aaggcacttg gtggcaattt tacccgccat gatggctggt ttgaaagtga taacgccatt gtgactaact gttttggtca tattatcgaa tcacaaccgc cggaaaacta taatcctgaa tacaaagcct ggaaggttga aacgcttcct ttacgtcttt atcccgtgaa gtatcagcct gttgaaagtg cagcaaaaca ggttaaaacg attctcgaac ttatcagacg tggagacgtg actgaaattg ttcacgctgg cgatcctgat gatgagggac agctacttgt tgatgaagtc ctggaatatg caggaaacac aaaacccgta aagcgcgttc tgataaacga caacacgctt ccggcagtga aaaaggcact ggcaaatctt aaagataatc gtgatttcaa agggctttac cttaaggcgc tggcgcgttc agttgccgat gccgtctatg gattctccat gacgcgtgct tacaccattc ctgcaaaagc cagaggatat cagggcgttc tgtctgtcgg gcgcgtccag acacccgttc ttggcctgat tgtgaatcgt acccgtgcta accagaacca taaatccagt ttttactaca ccatgaccgg agtttttcag cgtggtgctg atgttctcag ggcgaactgg aaaccaggtg aatttgctcc gctgaccgac cgtaaattac ttgataaggc gtgggcagac ggaacggcag catcccttgc aggaaaaccg gctacagttg aagcagcagc aactgatgat aaaaaaacgg ctgcgccgtt gccgtttaac ctggtcagac tccagcaata catgaacaag aagtttaaaa tgacggcaca aaaaacgctg 42180 42240 42300 42360 42420 42480 42540 42600 42660 42720 42780 42840 42900 42960 43020 43080 43140 43200 43260 43320 43380 43440 43500 43560 43620 43680 43740 43800 43860 43920 43980 44040 44100 44160 44220 44280 44340 44400 44460 44520 44580 44640 44700 44760 44820 44880 44940 45000 45060 45120 45180 45240 45300 45360 45420 45480 45540 45600 45660 45720 13 201142028 gatattacgc aacaactacg tgaaaaatat aaagcaatta cttataaccg ctcagattgc 45780 tcatatcttt ctgatgaaca attcagtgaa gcgccgcagg ttatcgatgc cctgaaatca 45840 gtctttcctc agtcgctgga tattgattct tcacgtaaaa gcaaagcgtt taacagtgca 45900 aaggtgactg cgcatactgc gataatcccg acctccagtg tgcctgatgt taacgcactc 45960 agcaccgacg agcgcaatgt ttacctggcg atcgcacaac actatcttgt tcagttcatg 46020 cctgaaaaag cataccagga agtatcggtt gccattcagt gtggtgatga gtcgttctat 46080 gcccgtgcca gaaaaacaac tgacagcgga tttgaggcgt ttcttggcgc ggaaatcaca 46140 gacgaaggtg aatcagaaga taatgatgat tccgcttttg aactgctctg taaaattcgc 46200 acaggagaaa cactgacgac aaaagaagtt gttgttaatg agaagaaaac aacaccgctg 46260 ccgttattca ccgaagcctc cttgcttgct gcgcttgttc gtgtcgcgga ttt 46313 <210> SEQ ID NO 2 <211>32,950 <212>DNA <213>腸炎沙門氏菌 <400>2201142028 ggtcaatact ggacaggact ggctaagttg cttcatttct ttattaacta tgagccgtcc tggcttaatg agttcgggct taaacccgtt ttctcaatcg gttctgtcgt cgacttgtac agcaatattg accgggaact gatactcagt aagcgggaag aactggaggg aacaaacggg cttgatgaaa acgcgttgta tcatttgcgc gatgccctga ccaaaatcag ggaatatcac gaaacggaag atgaacagcg ttcaagcatt gatggttctt tccgtaagaa aatgagcctg ttttatctcc caaccgttcg taaatgtact gatggtaatg atttcgatct ccgtcagttg cgacgggaag atatcactgt ttatgtcggt gttaatgcgg aagatatatc actggcttac gattttctga acctgttttt caacttcgtt gttgaagtga cattgcgtga aaatcctgat tttgatccca ccctgaaaca tgactgcctg atgtttcttg atgagttccc ttcgattggt tatatgccaa ttattaaaaa gggatcaggg tatattgcag gttttaaact taaactgctg acaatttatc agaatatcag tcagctaaat gaaatctatg gtattgaggg agccaaaacg ctgatgagtg ctcatccctg ccgtattatc tatgctgtca gcgaagagga tgatgccgcg aagatatcag aaaaacttgg gtatattacc actacatcaa agagcacaag caagaaccgg ggacgatcaa cttcacaggg cgaatcagaa agtgaagccc gaagagcact ggtgcttcca caggaactgg gaacgctgga ctttaaagaa gagtttatca tcctgaaggg ggagaaccct gttaaagcag aaaaggcact ttattacctt gatccgtatt ttatggacag gttaatgaag gtcagtccta aactggcatc attgacgatg aaactaaata agacgaaaaa aatatttggt gtgaaagggc ttaaatatcc gtcaaaagaa aaaatgctct ccgtaggaga gctggagtct gaggttttgc tatgaaaaaa atacttatga tcagtattct tgtcctcaca gcctgttcct ctccacctga accgccacag gttgaatggg aaaaaaggcc tgaagttatg aatacacaaa taatgaactg gaaaccaaca tccggtgtta ttaaatcaaa taatataaat tcctcatgga gtaaggtgtt gcctgatttt aaaccagaaa accatcttta cgatgattct gttttttatg ccgttgccca ttctgaaaaa atagttgtaa ggacatcttc ttttgatagt tactggtcag cgaaagactg gctaagaaaa aatggtgcaa caggtgttat tgaatatcag ccactaaaaa gatggttgaa taatgactat gttgaaattt atctgtcaag aataaatatt cagaggttac tatgaaaagg atatgcaaag gtctaatcgt aatttttact gtgtcctgtt ttatagcgcc aacgtatgcc gctgatccct gtaaatccgt tttttgtctt tatggtaaag ctgtcggtag tagtggaggg agtgagtgca gcagtgcaga aaaggatttt tttaaaaatg ttgaaaagaa aaaggggaaa atccgctggg gtaaaacatt tgatctccgt aagaattttt taaatcagtg ttcaacggca gacccggctg caatctcact gatcatgagt aagtttggtc gcgtcagagg ttgattaaaa cctgataccc gcgccaacgg gtatcagggg actgcaagta tcacataccg cgccaacgaa atgtgatgat tagttacaat cactgaggtt agaaatgaaa gtaaccaaat ctattatatc tattttattg tgcttatctg ctggtagtgc ataagtggat gtaataaaaa atgctcgtac ctttttgaaa ggaacccttg atctgagcgt gcaggaagca gatgaaaggg aagaacttta taagaagaac ggggcgcaac ctgactattt aagttacctg gaggattaac acatgcaata tgcattattt gatgggatgg aacgaaagtt tttgctggat gctcttgaat ttggtgttct gaaggactgg aaagaaaatc cggtaaaaga acttcctgat attgatgaat ctgttcaccc cttccatgtc tgttatggtg gatatttatt aaaccctgat gtttcagatt tagatattag cagaaaaata aaagaccaga caggattctg gctggcagct attgatgata cccgtatgga ttgtcattca atagcttatt atgatattca caccctccct ttaatttcgt gtggtcatca gaagatagtt ccttttgcag cgttaataaa agctgatgaa tgcatcattt caaaaattgc ttcgtattct ggttttgccg taacagcctt tttgagaatt aaagaccaga atatcgcaac gaatatactt aaccgtgagg gaatttttgc ctttaatggc tgtgagcaca gattcagaca acctgtaagt gaagataact ggcaacaggc agtgtcagaa gaacgcgcta tccgttgtgc caaaagatta attcaatgta aaggataaca aaatgagact ttttatcgct gaaaaacccg cagtagcaa a tgatattgtt aaggcacttg gtggcaattt tacccgccat gatggctggt ttgaaagtga taacgccatt gtgactaact gttttggtca tattatcgaa tcacaaccgc cggaaaacta taatcctgaa tacaaagcct ggaaggttga aacgcttcct ttacgtcttt atcccgtgaa gtatcagcct gttgaaagtg cagcaaaaca ggttaaaacg attctcgaac ttatcagacg tggagacgtg actgaaattg ttcacgctgg cgatcctgat gatgagggac agctacttgt tgatgaagtc ctggaatatg caggaaacac aaaacccgta aagcgcgttc tgataaacga caacacgctt ccggcagtga aaaaggcact ggcaaatctt aaagataatc gtgatttcaa agggctttac cttaaggcgc tggcgcgttc agttgccgat gccgtctatg gattctccat gacgcgtgct tacaccattc ctgcaaaagc cagaggatat cagggcgttc tgtctgtcgg gcgcgtccag acacccgttc ttggcctgat tgtgaatcgt acccgtgcta accagaacca taaatccagt ttttactaca ccatgaccgg agtttttcag cgtggtgctg atgttctcag ggcgaactgg aaaccaggtg aatttgctcc gctgaccgac cgtaaattac ttgataaggc gtgggcagac ggaacggcag catcccttgc aggaaaaccg gctacagttg aagcagcagc aactgatgat aaaaaaacgg ctgcgccgtt gccgtttaac ctggtcagac tccagcaata catgaacaag aagtttaaaa tgacggcaca aaaaacgctg 42180 42240 42300 42360 42420 42480 42540 42600 42660 42720 42780 42840 42900 42960 43020 43080 43140 43200 43260 43320 43380 43440 43500 43560 43620 43680 43740 43800 43860 43920 43980 44040 44100 44160 44220 44280 44340 44400 44460 44520 44580 44640 44700 44760 44820 44880 44940 45000 45060 45120 45180 45240 45300 45360 45420 45480 45540 45600 45660 45720 13 201142028 gatattacgc aacaactacg tgaaaaatat aaagcaatta cttataaccg ctcagattgc 45780 tcatatcttt ctgatgaaca attcagtgaa gcgccgcagg ttatcgatgc cctgaaatca 45840 gtctttcctc agtcgctgga tattgattct tcacgtaaaa gcaaagcgtt taacagtgca 45900 aaggtgactg cgcatactgc gataatcccg acctccagtg tgcctgatgt taacgcactc 45960 agcaccgacg agcgcaatgt ttacctggcg atcgcacaac actatcttgt tcagttcatg 46020 cctgaaaaag cataccagga agtatcggtt gccattcagt gtggtgatga gtcgttctat 46080 gcccgtgcca Gaaaaacaac tgacagcgga tttgaggcgt ttcttggcgc ggaaatcaca 46140 gacgaaggtg aatcagaaga taatgatgat tccgcttttg aactgctctg taaaattcgc 46200 acaggagaaa cactgacgac aaaagaagtt gttgttaatg agaagaaaac aacaccgctg 46260 ccgttattc a ccgaagcctc cttgcttgct gcgcttgttc gtgtcgcgga ttt 46313 <210> SEQ ID NO 2 <211>32,950 <212>DNA<213> S. Enteritidis <400>

o o o 6 2 8 1± IX oooooooooooooooooooooooooooooooooooo 406284062840628406284062840628406284 233445667789900122334556678899011223 11111111111111111222222 aaccaagaca accacacata aaaacaatat gctcactttt taaccactgc gcgtaattgc ctgatgttat ccttgtgctg ttccgcttcc tcgctcacta actcgctacg ctcggtcgtt cggctgcggc gagcggtgtc tcctttctca aaaccagaag agttcagatc atacgatccg ttgattgatc ctttttggat ctttcactga gaagccctga atcgctttct gtcgcttcgt tcgatacaac catggtgaaa ccatgcgaaa tcgaaaaaat cgctcagaat gcgatacagc gcgttttaga gggtatttac aaaacagtcg ttgccttgag gaaattttga tcaagattga actcctatcc ggatcatggc aacagatcac agcaacaagg ccgctaaaaa gcgttgagcg gcgttaaaca ccgtcaaacc gtagccgact acaacgaaac gggaaaaacg ctcagaacgc gttacagagc gttttaaggc gatgtcggaa agagccatat ctcacctgtt ctgatgtttt tctcaacatg agcactttta agagtgcttt taaagatgct attatgccaa tgagacaagc ggagggctta tgagctatca aattctgaca accacagcgg ccagtattac tgacctgaaa aaaaatccta tgggaaccgt agctgaaggt gaaggggacg ctgttgcgat cctgaaccga aacgaaccgg cgttctattg cgttccacca aaactttacg cctactatcg ggaactcgct gaagatgctg agttaaacgc tgttgctgat gagcgcatga aaaacccgga aattgtgaag gttaacctgg atgacctatg aactggcttt tgaccgtaga gcactgaagg aatggcagaa actcggccac accatccgtg aacaattcaa aaagaaactg gcagaacggc tggaaaatcc acgcgtaccc gcagcccggt tacatggtca tgctgatcgc tataaaatca aacttcgtgc atctggctac agacttgtat atcaagtcat tgatgagaaa gtcgttttac ttgttatttc cgttggaaga agggaaagca gcgaagtcta tcagatcgca gatttgcgct aageittaatc atcaaaggat taactgttta ctgtcagaac aagagcatta aaaatttgcc acacctgcaa atgcaggtat aataaaacac aagaaaggga aacaggttct ctttcatcca gagcccaaag tgggcgggag gttaaaaatg caaaactacg ctaagtctgt agccacagag attttacgtc aacttggtgg taatcgtttt attgttatga ctggtgctaa aagtttttct tactttgatg aaaacggtga gtgcgggtta actttccgtt tgccgtccaa ttttgcaatg aaaggcatca acttagtaaa aattaaactg gattttactg atacgtacca ggtgaaattt tctcgtgtac ggggtgatga agttaaagat atttcaagat tcgataatat ctattgtgat cagttagcgt gtttatttac acaagaaaca gggttacata ccgtgttata gataaaaagg ggcattaagc ccctttttta gttattatga ggaataatat ggataaagat aagataatta agaaaaacag aggtaattac tcatatgtaa tcagaacgat ggatgaagat ggggatgcgg tttttcacgt cttaaaatat gttaagacga ttgataaaac taaaagcagg aaaacagtaa gaaaattgat aatggacgaa aaactcaacc tggcatcatt gatgcttctg gataatggga ttttgtgtga ttgtctgaca aaaggggatg aaaatgcaga ataaacctac acctgaagaa gtaaagaatg cacgggttgc ggcaggtctt actcttaaag aagctgctga tatttttggt tatcaactga attcctggca gatgaaagaa agtgcaggta aggccagtcg ttctttatct gttggtgaat atcagtattt attgctatta gcaaatatgc atccgtctta caggctggta aaaaaataac ttgctatacc tgcaaatgca ggtataataa aacacaagaa agggaaacag gttctctttc atccagagcc caagctgggc gggagctaaa aatgaaagta ttaactttta aaaatgatac tgtctctgtt ggtgatgtct ttgtatcatc ctggggctat gagcaaacca atgtaacctt ctaccaggtt ctttctgttc acggtaaaaa aaccgtcacc gttcgcgaga ttcgcgctaa 14 201142028ooo 6 2 8 1 ± IX oooooooooooooooooooooooooooooooooooo 406284062840628406284062840628406284 233445667789900122334556678899011223 11111111111111111222222 aaccaagaca accacacata aaaacaatat gctcactttt taaccactgc gcgtaattgc ctgatgttat ccttgtgctg ttccgcttcc tcgctcacta actcgctacg ctcggtcgtt cggctgcggc gagcggtgtc tcctttctca aaaccagaag agttcagatc atacgatccg ttgattgatc ctttttggat ctttcactga gaagccctga atcgctttct gtcgcttcgt tcgatacaac catggtgaaa ccatgcgaaa tcgaaaaaat cgctcagaat gcgatacagc gcgttttaga gggtatttac aaaacagtcg ttgccttgag gaaattttga tcaagattga actcctatcc ggatcatggc aacagatcac agcaacaagg ccgctaaaaa gcgttgagcg gcgttaaaca ccgtcaaacc gtagccgact acaacgaaac gggaaaaacg ctcagaacgc gttacagagc gttttaaggc gatgtcggaa agagccatat ctcacctgtt ctgatgtttt tctcaacatg agcactttta agagtgcttt taaagatgct attatgccaa tgagacaagc ggagggctta tgagctatca aattctgaca accacagcgg ccagtattac tgacctgaaa aaaaatccta tgggaaccgt agctgaaggt gaaggggacg ctgttgcgat cctgaaccga aacgaaccgg cgttctattg cgttccacca aaactttacg cctactatcg ggaactcgct gaagatgctg agttaaacgc tgttgctgat gagcgcatga aaaacccgga aattgtgaag gttaacctgg atgacctatg aactggcttt tgaccgtaga gcactgaagg aatggcagaa actcggccac accatccgtg aacaattcaa aaagaaactg gcagaacggc tggaaaatcc acgcgtaccc gcagcccggt tacatggtca tgctgatcgc tataaaatca aacttcgtgc atctggctac agacttgtat atcaagtcat tgatgagaaa gtcgttttac ttgttatttc cgttggaaga agggaaagca gcgaagtcta tcagatcgca gatttgcgct aageittaatc atcaaaggat taactgttta ctgtcagaac aagagcatta aaaatttgcc acacctgcaa atgcaggtat aataaaacac aagaaaggga aacaggttct ctttcatcca gagcccaaag tgggcgggag gttaaaaatg caaaactacg ctaagtctgt agccacagag attttacgtc aacttggtgg taatcgtttt attgttatga ctggtgctaa aagtttttct tactttgatg aaaacggtga gtgcgggtta actttccgtt tgccgtccaa ttttgcaatg aaaggcatca acttagtaaa aattaaactg gattttactg atacgtacca ggtgaaattt tctcgtgtac ggggtgatga agttaaagat atttcaagat tcgataatat ctattgtgat cagttagcgt gtttatttac acaagaaaca gggttacata ccgtgttata gataaaaagg ggcattaagc ccctttttta gttattatga ggaataatat ggataaagat aagataatta agaaaaac ag aggtaattac tcatatgtaa tttttcacgt tcagaacgat ggatgaagat ggggatgcgg ggtataataa aacacaagaa agggaaacag cttaaaatat gttaagacga ttgataaaac taaaagcagg aaaacagtaa gaaaattgat aatggacgaa aaactcaacc tggcatcatt gatgcttctg gataatggga ttttgtgtga ttgtctgaca aaaggggatg aaaatgcaga ataaacctac acctgaagaa gtaaagaatg cacgggttgc ggcaggtctt actcttaaag aagctgctga tatttttggt tatcaactga attcctggca gatgaaagaa agtgcaggta aggccagtcg ttctttatct gttggtgaat atcagtattt attgctatta gcaaatatgc atccgtctta caggctggta aaaaaataac ttgctatacc tgcaaatgca gttctctttc atccagagcc Caagctgggc gggagctaaa aatgaaagta ttaactttta aaaatgatac tgtctctgtt ggtgatgtct ttgtatcatc ctggggctat gagcaaacca atgtaacctt ctaccaggtt ctttctgttc acggtaaaaa aaccgtcacc gttcgcgaga ttcgcgctaa 14 201142028

ttcagaatat accgattcaa tggtcggctt taaaactccc gttttaaatg attttactgg tgaatgtttt aagcgccaga taaaagattt tggtgatgag ctggcaatca aaatcgaaga ttttgaaact gcgtataaaa ctctaccgga agaaaaacat cgattttctt cttactactg attaatcagg gggatattcc cccttttagt aaaggtctga aaatggaaag agagtttagc gcaaaagcat cattaaaccg aaatataaaa ttttggtttg agcaatgtgg gttatctaaa gaaagagtta ttcgttgtat tgataactgg tatgaccttg catacccacc atcagaacag gagaaagcaa aaaaagaagc aattgagaag ttaataaagt aaagataaag atggtatcag gttgtggggc aacctgatac cgtgacacct gattctattg cgaggaataa aatgtcggaa cgcatattat cagcaataaa tgacgttgaa aagggtgggc gtccggtttt ccctttgatg ccattccatg tctttcctga gtatatggca ttactcagaa aagcactgga aaaaaagaca caaaagagaa cagataaata aaccggaggc tttatgacaa aacatgattt tgtgtccttt gtcagtggag aattgcggca aggcgctgta cgcttttctc ttgcttttaa cagcaaagga gaaattgttc tgcactggac taataaagca ggaatacgag tatggcggat attaagtggt aatcgtggca aaaaaccaag caaagctaac cttgaaagaa tgagtaactt ccggcgctgg ctttttgatg cccgtcaggg catggaaggc tacactcagc aacctgaaca gagcaacctt agctgaatag ccgaagccct cccgtatttc gggaggtgct ttctgatata ttgcgcggga ctgcgcttgc gcttcctgcc agaccgttcg cggagcgaac ctgacggcac tgtccttacc gcattgatcg ggtttccggt cgcagacgta cgtatgttca gccatgacaa catcagcctt tttgcatttt tgttgatgct caagcacaat ttatgagcgg, tgccagattc actgcgatgc aggatagggc aaacgccgca aaatgacgtc tctgacgcca ttccgcaacg tttgcccggt gacctacagt cggttattgt cggagaattt tatgaaaaaa gttcaattca gaattgatga aaatcagcat aatgatttgc tggattgtct taaaactctt tatccagatg aaccagcttt aacagtagct aaaggcatga aacttttagc aaatgcttta ttaaaaagta aagctggcag taaggacata aatacgtttt ttgataataa tgattttatc aaaacaacga tgtacttaac aggtaaacaa agggctgata ttgaaagagc tgctaatcgt cacggatgga cgttatcacg agaatgtcgt taccgcatac agacgacact tgaaaatgaa ctggatttct ttgaccagga actgctgatg atgaatcgtt gccgtaattc aattgataag atcggtcgta atttccatta tatcattgtt aatgatcaga ccagggttct tgataaagat ggtttctatc aggatgcgga gcgtcttaca acagaaattt ttaatcttaa gaatcagttt gagaattaca ttatgttatg taaagggaga actgtttcaa ataaagtgga gatgtaatta tgggcgttta cgttgataaa gaatatcgtg ttaaacgaaa gtcatcagaa aatggtcgta agtcagcttt cgctcacaaa gtcaaaaatg gtggaaagaa ctatagccgc aatgttcagg aacgtatcaa ccgcaagggt gccagtaagg aggttgttgt caaaatatct ggaggtgctg ttactcgtca ggggattcgg aacagtattg attatatgag ccgtgagtca gagctaccag tgatgagtga aagcggtcgg gtatggatgg gtgccgaaat tctggaggct aaagagcaca tgatagatcg tgctaatgat cctcagcatg tgatgaatga taaaggtaaa gaaaataaaa aaatcacaca gaatattgtc ttctcgcctc cagttttagc gaaagtaaag cctgaagatt tgttggagtc tgtcaggaaa acgatgcaga aaaaatatcc taatcaccgt tttgttcttg gataccactg tgacaagaaa gaacatcctc acgttcatgt tgtttttcgt atccgagata atgacggtaa acgcgctgat atcaggaaaa aagatttacg ggaaattcgt acaggttttt gtgaagagtt gaagttaaaa ggttatgacg ttaaagcgac ccataagcaa cagcatggac ttaatcagtc tgttaaagat gcacataata cagcaccaaa aagacagaaa ggtgtttatg aggttgttga tattggctat gaccattatc agaacgataa aacaaagtct aagcaacatt ttataaagct aaagactctt aacaaggggg ttgagaaaac atactggggg gctgattttg gggacttatg ttcgcgggaa agtgttaaag caggtgatct tgtcaggctg aagaaacttg gtcagaaaga agtaaaaatc ccggcgctcg ataaaaacgg tgttcagcat ggctggaaaa cggttcacag aaatgagtgg cagttagaaa atctgggggt taagggcgta gacagaacac cttcagccag caaagagctg gtacttaaca gccctgatat gctgctgaag caacaacagc gaatggcgca gtttacgcag cagaaagcat ccacgttaca gtcagaacag aagctgaaaa cagggattaa gtttttgggc ttataagatt taatgtattg attatgcgag aaaaatattt attttagttc gcttgctcat cttttcacct taaaaacaga tcgaaattct gcgttttagc tatcattcga tctatcgatc taaaaaacag atctgcaaaa gatgaatatt ctgtgtttca ttgcatacat ggaataaatg aaagtaaaat tgatttatca agatcgaaag atctgtttta tgtgttgaac ttagcataag ccacttgtag tataaacttc gcgcaatagt aaaaagtctc tcttcatagg ggcgtgattt ctcacaagca accacggttg gcttgtgttt tgtcacatct gtatatcggg gattgagtat gaaaggacag aaacagtttt acctgcttca gtgttctccg cattttcatc agtcggttct gttgcagtta tcccggagcg gtatcgctta ctacaatcca gttatacgca ctttctataa gaggcgtgac tgtagtgcat acaggggaca aatcatgcca atgtttcccg gttatgtatt cgttcttctg gattttgagg ttgtacatcc atcctgcttt acccggatga aaaatgttta tggactggtc agttttggtg aatatcctgc tgaagttcct ctgagtgtca taaatgaagt 15 201142028 aaaagagcag gagaagattt tttcgatgaa tttaagcctg atgaataact cctgtctggc aaaaatactt ctccttgcag acgcttcaaa gcgtggacga gtctttgccg actatgtgtg taacagaaaa tacgacaggt taaaaaatga cagctataaa aggaaagcgg aaaccgcaac gcaatgtact ctaccttcct acagaagttc gtgttgaagt tgaaaaaatt gcaattgaaa taagcttcaa gagggggcga cgtatctctg attetggttt tgttcagtat cttattaaaa aatacaaatc tcaggcgatg aaagaactta ttcatggggc tgatattcct gacgagtaat attgtcccaa tgttctgaat gtggaatatt tatgttatct acttctactt ttcttgcgct tgccatgcaa tgcgctgcca gcgttcatcc cgacacaacg cacgaagtcg ccagggttga atcaggtttt aacccatatg cgattgcgga aataatacca aaggttaaac gtaaaactgg tgataaaggc gtagtatctt actttcctga atcaaaggag gcagcactta agatcgttaa aaacattgaa ttacggaatc atcgttactc tgtaggactt atgcaaataa cgagtaccaa ttttgcaaag ttcggtacaa cagcagagaa aatgtttgat ccatgcgaaa atcttaaggt atcagaaaaa atactggttg actgttataa acgaggtggc gacttagtgc gtgggctgag ttgctattat tctggcaatc aagaaacagg agtaaagcca gaacctgaat ttaataatac aagttatgta caacgtatag gatttagccc tcctgataat aaaaaaagtt ttattgttcc ctctgtaaag gaaatgatta aaaaggagaa taagacgact atcacacctg aagaaatcat tatatatcct caatacgcca tgcgtggcac tgtatcaaat gaaaaggaaa caaaagatgt tgaaattaaa tctgaataaa cgttatttaa cgctttctct atttatggct gcgttgatgc tttgtgttgc agaacctgct tttgcggatg atgtgtctac gaagacaact ggttttttac agaaaataat tgatttttta acggatattc gtaaacctgc aattacaatt attgctcttg ttattgggta tattgcgata ttttctcgtc aacatacctc atggatagtc ccacttgtta tcggaattat catctttatt gttgcaccat atattcctga ctggcttgcg taattaaagg atgggcgcac tatgagtact ctttttaaag gtcttacgcg ccctgcttta ataagggggc tgggcgttcc gctctacccc tttcttggaa tgtgcattat ttgtgttctg cttggtgtct ggattcatga ggctatgtat gcccttattc ttcctggctg gtatgccatc aggcgtgtaa cacagtttga tgaacgcttt tttgaccttc tgtatctgag aactcttgtc aaagggcatc ctttatcaaa caagcgattc agcgcagtcc attatgcggg gagccagtac aatgaagttg atatttcaaa agtggataac tttatgaagc tgaaagacca gtcttctgtt gaagagttaa ttccgtactc ttcacatatc actgataata ttatcgttac aaaaaaccgg gatttgctgg caacctggca gatagacggt gcttactttg agtgtgttga ttctgaagat ttgtcaattc tgacagatca gcttaatacg cttatacgta gctttgaagg gaaatttgtt acgctttatc ctcatcgtat caggtgtaaa aagggcgtca gaccagtatt taacagtaaa attccttttg tgaacagagt aatgaatgat tattacgagt cattccctca gtctgaattt ttcgagaata aattatttct gacgatttgt tttaaacctt ttactacgga agataaagta acacatttct tttcacgcag taaaaaacaa aaagatatct ttaaagagcc tgttaatgaa atgaatgaaa tttgcgacag gttgaatacc tatctgtccc gttttcattc ccgacgtctt gggctttatg aagatcatgg ggttgtttat tcagatcagt tatctctgtt ccagtatctg ctttctggtc gatggcaaaa ggtcagggtt agcagtagtc cgttttatac atatctggga ggaaaagacc tgttctttgg taatgatgcc ggacaaatta ccgcgtcaga ccatgcccgg tattttcgtt gcatagagat taaggattat tttcaggaga cggatgccgg tattctggat gctctgatgt atctccccgt tgagtatgtc gtgacatcgt cctttactgc gatggataag cagtcagcga ttaaggcgct ggatgatcag atcgataagc tggaaatgac agatgatgct gccaaatctt tgctggcaga tctgaaagtc ggactggata tggtttccag tggatatatt tctttcggaa aatcgcatca gaccctggtt gtctttgcgg attcaccgga gcggctggtg aaagacacca atatcgtgac ttccactctg gaagatttgg ggctgattgt cacttattca acactgagtc ttggcgcagc ttattttgct cagctaccag gaaattatac gcttcgccct cgtctgagta ccctcagtag tcttaatttt gccgaaatgg aaagttttca taatttcttt tcaggaaaag aaaaaggaaa tacctggggg gaaaaactga ttactcttcg ggggtcaggt aatgatatct accatctgaa ttaccatatg acgactgaac atcagaattt cttcggtaag aacccgacgc tggggcatac cgaaattctc ggtacgtcta acgtgggtaa aaccgtatta ctgatgacaa aagcatttgc cgcccagcag ttcggtacgc cggaatcatt ccctgcaaac agaaaactga aaaaactgac cacggttttt tttgataaag accgggcagg tgaagtcggt atacgggcaa tggggggatc ttattaccgg gtgaaggagg gagagccgac aggctggaat cccgccgcac tgccgccaac aaagcgtaat atcgctttta tgaaggacct ggtgaggctg ctttgtactc tcaacagtga gccgctcgat gattaccaga acagcctgat ttcagatgcg gttgaacgtc ttatgcaacg gtcagatcgc tcttatcctg tcagtaaact acggcctctt atccaggagc cggatgatac tgaaaccaaa cgtcatggac ttaaagcccg tcttaagccg tggacgcagg gggaagagtt tggctgggtg ttcgacaatc gggaagacac gtttgatgtc gataacctgg atgttttcgg tattgatgga acggagttcc tggataataa ggtgctggcc agtgctgctt cattctatct catctatcgg gtcaccatgc tggccgatgg tcgcaggctt cttatctaca oooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo 062840628406284062840628406284062840628406284062840628406284 001123344566778990012233455667889901122344556778890011233445 666666666666666667777777777777777788888888888888889999999999 16ttcagaatat accgattcaa tggtcggctt taaaactccc gttttaaatg attttactgg tgaatgtttt aagcgccaga taaaagattt tggtgatgag ctggcaatca aaatcgaaga ttttgaaact gcgtataaaa ctctaccgga agaaaaacat cgattttctt cttactactg attaatcagg gggatattcc cccttttagt aaaggtctga aaatggaaag agagtttagc gcaaaagcat cattaaaccg aaatataaaa ttttggtttg agcaatgtgg gttatctaaa gaaagagtta ttcgttgtat tgataactgg tatgaccttg catacccacc atcagaacag gagaaagcaa aaaaagaagc aattgagaag ttaataaagt aaagataaag atggtatcag gttgtggggc aacctgatac cgtgacacct gattctattg cgaggaataa aatgtcggaa cgcatattat cagcaataaa tgacgttgaa aagggtgggc gtccggtttt ccctttgatg ccattccatg tctttcctga gtatatggca ttactcagaa aagcactgga aaaaaagaca caaaagagaa cagataaata aaccggaggc tttatgacaa aacatgattt tgtgtccttt gtcagtggag aattgcggca aggcgctgta cgcttttctc ttgcttttaa cagcaaagga gaaattgttc tgcactggac taataaagca ggaatacgag tatggcggat attaagtggt aatcgtggca aaaaaccaag caaagctaac cttgaaagaa tgagtaactt ccggcgctgg ctttttgatg cccgtcaggg catggaaggc tacactcagc aacctgaaca gagcaacctt agctgaatag ccgaagccct cccgtatttc gggaggtgct ttctgatata ttgcgcggga ctgcgcttgc gcttcctgcc agaccgttcg cggagcgaac ctgacggcac tgtccttacc gcattgatcg ggtttccggt cgcagacgta cgtatgttca gccatgacaa catcagcctt tttgcatttt tgttgatgct caagcacaat ttatgagcgg, tgccagattc actgcgatgc aggatagggc aaacgccgca aaatgacgtc tctgacgcca ttccgcaacg tttgcccggt gacctacagt cggttattgt cggagaattt tatgaaaaaa gttcaattca gaattgatga aaatcagcat aatgatttgc tggattgtct taaaactctt tatccagatg aaccagcttt aacagtagct aaaggcatga aacttttagc aaatgcttta ttaaaaagta aagctggcag taaggacata aatacgtttt ttgataataa tgattttatc aaaacaacga tgtacttaac aggtaaacaa agggctgata ttgaaagagc tgctaatcgt cacggatgga cgttatcacg agaatgtcgt taccgcatac agacgacact tgaaaatgaa ctggatttct ttgaccagga actgctgatg atgaatcgtt gccgtaattc aattgataag atcggtcgta atttccatta tatcattgtt aatgatcaga ccagggttct tgataaagat ggtttctatc aggatgcgga gcgtcttaca acagaaattt ttaatcttaa gaatcagttt gagaattaca ttatgttatg taaagggaga actgtttcaa ataaagtgga gatgtaatta tgggcgttta cgttgataaa gaatatcgtg ttaaacga aa gtcatcagaa aatggtcgta agtcagcttt cgctcacaaa gtcaaaaatg gtggaaagaa ctatagccgc aatgttcagg aacgtatcaa ccgcaagggt gccagtaagg aggttgttgt caaaatatct ggaggtgctg ttactcgtca ggggattcgg aacagtattg attatatgag ccgtgagtca gagctaccag tgatgagtga aagcggtcgg gtatggatgg gtgccgaaat tctggaggct aaagagcaca tgatagatcg tgctaatgat cctcagcatg tgatgaatga taaaggtaaa gaaaataaaa aaatcacaca gaatattgtc ttctcgcctc cagttttagc gaaagtaaag cctgaagatt tgttggagtc tgtcaggaaa acgatgcaga aaaaatatcc taatcaccgt tttgttcttg gataccactg tgacaagaaa gaacatcctc acgttcatgt tgtttttcgt atccgagata atgacggtaa acgcgctgat atcaggaaaa aagatttacg ggaaattcgt acaggttttt gtgaagagtt gaagttaaaa ggttatgacg ttaaagcgac ccataagcaa cagcatggac ttaatcagtc tgttaaagat gcacataata cagcaccaaa aagacagaaa ggtgtttatg aggttgttga tattggctat gaccattatc agaacgataa aacaaagtct aagcaacatt ttataaagct aaagactctt aacaaggggg ttgagaaaac atactggggg gctgattttg gggacttatg ttcgcgggaa agtgttaaag caggtgatct tgtcaggctg aagaaacttg gtcagaaaga agtaaaaatc ccggcgctcg ataaaaacgg tgttcag cat ggctggaaaa cggttcacag aaatgagtgg cagttagaaa atctgggggt taagggcgta gacagaacac cttcagccag caaagagctg gtacttaaca gccctgatat gctgctgaag caacaacagc gaatggcgca gtttacgcag cagaaagcat ccacgttaca gtcagaacag aagctgaaaa cagggattaa gtttttgggc ttataagatt taatgtattg attatgcgag aaaaatattt attttagttc gcttgctcat cttttcacct taaaaacaga tcgaaattct gcgttttagc tatcattcga tctatcgatc taaaaaacag atctgcaaaa gatgaatatt ctgtgtttca ttgcatacat ggaataaatg aaagtaaaat tgatttatca agatcgaaag atctgtttta tgtgttgaac ttagcataag ccacttgtag tataaacttc gcgcaatagt aaaaagtctc tcttcatagg ggcgtgattt ctcacaagca accacggttg gcttgtgttt tgtcacatct gtatatcggg gattgagtat gaaaggacag aaacagtttt acctgcttca gtgttctccg cattttcatc agtcggttct gttgcagtta tcccggagcg gtatcgctta ctacaatcca gttatacgca ctttctataa gaggcgtgac tgtagtgcat acaggggaca aatcatgcca atgtttcccg gttatgtatt cgttcttctg gattttgagg ttgtacatcc atcctgcttt acccggatga aaaatgttta tggactggtc agttttggtg aatatcctgc tgaagttcct ctgagtgtca taaatgaagt 15 201142028 aaaagagcag gagaagattt tttc gatgaa tttaagcctg atgaataact cctgtctggc aaaaatactt ctccttgcag acgcttcaaa gcgtggacga gtctttgccg actatgtgtg taacagaaaa tacgacaggt taaaaaatga cagctataaa aggaaagcgg aaaccgcaac gcaatgtact ctaccttcct acagaagttc gtgttgaagt tgaaaaaatt gcaattgaaa taagcttcaa gagggggcga cgtatctctg attetggttt tgttcagtat cttattaaaa aatacaaatc tcaggcgatg aaagaactta ttcatggggc tgatattcct gacgagtaat attgtcccaa tgttctgaat gtggaatatt tatgttatct acttctactt ttcttgcgct tgccatgcaa tgcgctgcca gcgttcatcc cgacacaacg cacgaagtcg ccagggttga atcaggtttt aacccatatg cgattgcgga aataatacca aaggttaaac gtaaaactgg tgataaaggc gtagtatctt actttcctga atcaaaggag gcagcactta agatcgttaa aaacattgaa ttacggaatc atcgttactc tgtaggactt atgcaaataa cgagtaccaa ttttgcaaag ttcggtacaa cagcagagaa aatgtttgat ccatgcgaaa atcttaaggt atcagaaaaa atactggttg actgttataa acgaggtggc gacttagtgc gtgggctgag ttgctattat tctggcaatc aagaaacagg agtaaagcca gaacctgaat ttaataatac aagttatgta caacgtatag gatttagccc tcctgataat aaaaaaagtt ttattgttcc ctctgtaaag gaaatgatta aaaaggagaa taa gacgact atcacacctg aagaaatcat tatatatcct caatacgcca tgcgtggcac tgtatcaaat gaaaaggaaa caaaagatgt tgaaattaaa tctgaataaa cgttatttaa cgctttctct atttatggct gcgttgatgc tttgtgttgc agaacctgct tttgcggatg atgtgtctac gaagacaact ggttttttac agaaaataat tgatttttta acggatattc gtaaacctgc aattacaatt attgctcttg ttattgggta tattgcgata ttttctcgtc aacatacctc atggatagtc ccacttgtta tcggaattat catctttatt gttgcaccat atattcctga ctggcttgcg taattaaagg atgggcgcac tatgagtact ctttttaaag gtcttacgcg ccctgcttta ataagggggc tgggcgttcc gctctacccc tttcttggaa tgtgcattat ttgtgttctg cttggtgtct ggattcatga ggctatgtat gcccttattc ttcctggctg gtatgccatc aggcgtgtaa cacagtttga tgaacgcttt tttgaccttc tgtatctgag aactcttgtc aaagggcatc ctttatcaaa caagcgattc agcgcagtcc attatgcggg gagccagtac aatgaagttg atatttcaaa agtggataac tttatgaagc tgaaagacca gtcttctgtt gaagagttaa ttccgtactc ttcacatatc actgataata ttatcgttac aaaaaaccgg gatttgctgg caacctggca gatagacggt gcttactttg agtgtgttga ttctgaagat ttgtcaattc tgacagatca gcttaatacg cttatacgta gctttgaagg ga aatttgtt acgctttatc ctcatcgtat caggtgtaaa aagggcgtca gaccagtatt taacagtaaa attccttttg tgaacagagt aatgaatgat tattacgagt cattccctca gtctgaattt ttcgagaata aattatttct gacgatttgt tttaaacctt ttactacgga agataaagta acacatttct tttcacgcag taaaaaacaa aaagatatct ttaaagagcc tgttaatgaa atgaatgaaa tttgcgacag gttgaatacc tatctgtccc gttttcattc ccgacgtctt gggctttatg aagatcatgg ggttgtttat tcagatcagt tatctctgtt ccagtatctg ctttctggtc gatggcaaaa ggtcagggtt agcagtagtc cgttttatac atatctggga ggaaaagacc tgttctttgg taatgatgcc ggacaaatta ccgcgtcaga ccatgcccgg tattttcgtt gcatagagat taaggattat tttcaggaga cggatgccgg tattctggat gctctgatgt atctccccgt tgagtatgtc gtgacatcgt cctttactgc gatggataag cagtcagcga ttaaggcgct ggatgatcag atcgataagc tggaaatgac agatgatgct gccaaatctt tgctggcaga tctgaaagtc ggactggata tggtttccag tggatatatt tctttcggaa aatcgcatca gaccctggtt gtctttgcgg attcaccgga gcggctggtg aaagacacca atatcgtgac ttccactctg gaagatttgg ggctgattgt cacttattca acactgagtc ttggcgcagc ttattttgct cagctaccag gaaattatac gcttcgccct c gtctgagta ccctcagtag tcttaatttt gccgaaatgg aaagttttca taatttcttt tcaggaaaag aaaaaggaaa tacctggggg gaaaaactga ttactcttcg ggggtcaggt aatgatatct accatctgaa ttaccatatg acgactgaac atcagaattt cttcggtaag aacccgacgc tggggcatac cgaaattctc ggtacgtcta acgtgggtaa aaccgtatta ctgatgacaa aagcatttgc cgcccagcag ttcggtacgc cggaatcatt ccctgcaaac agaaaactga aaaaactgac cacggttttt tttgataaag accgggcagg tgaagtcggt atacgggcaa tggggggatc ttattaccgg gtgaaggagg gagagccgac aggctggaat cccgccgcac tgccgccaac aaagcgtaat atcgctttta tgaaggacct ggtgaggctg ctttgtactc tcaacagtga gccgctcgat gattaccaga acagcctgat ttcagatgcg gttgaacgtc ttatgcaacg gtcagatcgc tcttatcctg tcagtaaact acggcctctt atccaggagc cggatgatac tgaaaccaaa cgtcatggac ttaaagcccg tcttaagccg tggacgcagg gggaagagtt tggctgggtg ttcgacaatc gggaagacac gtttgatgtc gataacctgg atgttttcgg tattgatgga acggagttcc tggataataa ggtgctggcc agtgctgctt cattctatct catctatcgg gtcaccatgc tggccgatgg tcgcaggctt cttatctaca oooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo 06284 0628406284062840628406284062840628406284062840628406284 001123344566778990012233455667889901122344556778890011233445 666666666666666667777777777777777777788888888888888888999999999999 16

201142028 tggatgagtt ctggcaatgg atcaataacg aagcgttcag ggactttgtt tacaacaagc tgaaaaccgg acgtaaactc gatatggtgc ttgtcgtagc cacacagtca ccggatgaac tgattaaatc acccattgcg gcagcggttc gtgagcaatg cgccactcat atctatctgg caaacccgaa agccaaacgt agtgaatatg ttgatggttt gcaggtcagg gagctttatt ttgacaaaat taaagctatc gatccgctgt cccgccagtt ccttgttgtt aagaacccac agaggaaagg tgaaagtgat gattttgctg cttttgccag actggagctg ggaaaagcag cgtattactt accggttctc agtgcatcaa aaccccagtt agaactgttc gatgaaatct ggaaagaagg aatgaagccg gaagagtggc ttgataccta tctggaacag gcgaacctga tttgaggaat caccaatgaa aaagcaaatt atggcggcat tcgtcgcttc actgattgtt atttccggcg ctcaggcagg gatacctgtt gccatcgacg ccaaccctga atgggcgatt gaagccggac gatggacaga acgccttaag caatgggcgg aaacggtaaa acattacgaa aatcagataa atgcgtacaa acaggagctg ctgtcaaaaa cgggtatccg tgatgtgcag ggactggtgc agtccgcaca gtcagtgagt caggaactga tgcagattta tgatcagggg aatgctttta ttgacgatta cattaaaaat cctgaagggg cgttatcgga acaggccaaa tcgttattgt cagattacaa agtaacggat acctgccaga acctgggata ttccggcgac ctggtacggg gatgtgaagc gacgttcctt tctcaactgg caagcgtgga atacggtaac aagctggaga gcaagcttcg tcaggacaac cagacgatga aagaccttat tgatcaagtc aaaaatgcga aggatacgaa ggccacgcag gatgcaacaa acgctgttgc acttgaacaa ctgaagttcg agaagctcaa atttcagtat caaatgtatc gcgataagca gcgagatctt gcagaatata aagagaagat ggctcaggca gctttcagaa aacagcaacg tgaagccgtg ccaccttctt acagaaaagc ttatatggca atgaaatcat atgaggatga ttaaatgaaa agtatcgcta ctgctggcct tgtcattata tctgttttta gtctttctgg atgttttgaa gaaacaaaat cggttgattg gtggcttgca catccgaagg agacatacaa aaaatttgaa gagtgtcaga agtctggtag cgattctgat aattgtaaga acgttaagcg agcacatttg tcttttgaac ggagaaaggc ggttggttta ccaataaatt gagggtgatg tatggggatt gtcactggta taccggatgg tactgattta tcaggtaatg taacctctcc cgcaaatgcg ggaggtgttt ctggatttga ttctgatttt tttcagacaa ctcatgaagt tatatttaat attctcaaca agagtatatc tggaaaatta agtgaatatt cagatgtggc ttatactctt ggtaaatatg gagtttctct gtatgtttta tggtatgctt ttactgtatt agcaaggaaa caacagacac ctgtacctga ttttatctgg aatatctgta ggttttacat aatattgctt tttgttaaga atacaggggg aatacttaca tcagcaacag atgctattga tggattgaaa aatacattgg cagggggaga tccgtgggta tggatggatc agttatgggt gaaggttata caagttgcaa ctcttatttt tgataaagat acatctactg tgcctgttgc cggtgggatt ggtgctttat taacttatgt cggtggtgtt ttggcattat tgctttgttc tatagtattt gcatctgctg aattaacatt actattactt tctgtcactg cgccaatatt tatcatgtgt ctgatgtttg gtttacttcg gcaaatgttt aatagctggc tacagcttaa ttttagctcg ttactggttt ttctatttgc agcattagca ctaagagctg ggacatggca attaaacatg gcattaagta cgtctattgc tacagcatca gaaaacaatc ttcttcaaac gggagtaact tcattagctg ctggcatttt catggcctgg attatctggc aggcgaaaag ttatgcttca cagattgcag gtgtgggtgt tgaaggtgcc atgcagggcg cagccgctat ggggattggt gctggcgttt tcggtgcatc ccgtatggcg cgtggcgcac ttggtatggg cagaaatgcc ggtattggtg catggaaagg tctgcgtcgt caggaagacg ggtttggtca gtctccggga ataacgggta agaccgctaa cctggccgga cagggcgtta atattggtgc caaaaagctt cgtcaggcag ctattgagag agcaaagaaa atgtatggtg ggtaatttaa ttatgagcgc cttttaatgt tcttttcata attgtcattg tcgggcctgg cgcgctctat tatcttttct gttcacactg agtcttctgt aaatattaat ctgttataca ggtgttcata ttatgaaact acttattgtg gctttcgtga cgctttgtct cgctggttgt caggcgtcac ataaactacc gcccgtttcc gggaaaagcg aacctgttaa ttctgctgag gtaatgcaaa atggaattta aacttcccgg atttaaaaat aaaaaagacg ttactgactc atcagtttca tttgaagaaa aaaacattgc actacaggag agaatgaatc gtatttataa attcggtggt atcggaggca tgttaattgg tgggctgtct ttacttgcat taaatgcagc attaccactg aaaacaacag ttgttgatgc ctaccttatc gataaggtta caggtgtggc tgaacgtctg acttctgtta aaaaagaaaa tctttctgaa aacgaagcca ttgcccgata ttttatcacc cagtatataa aacatcgtga aggttataat tttttcagtc tccagcatga ttatgattat gtaatggctt acagcgcgga gaatgtcgcg gcagattata acgcattatt taacagtgaa caggcaccaa aacttgttta taacaaagca gaaaaaacgg caatggttca ggataatcca tctgtcataa tttcaccttc gtcacgggca gatgataaag atatcggtgc gtatattcgt tttcgtctga ccatcaggga tgttgctacc ggacaaaccc gccaggagtt ctggaatgtt cgcctgactt atcgtatcga accgcaggtt gaaatggtgt caggggaacg taataacaat cctcttaaat tcgttgtaac aagctacgtt cgcgataaag aagccagagg ttaataatat gaaaatgaat 9600 9660 9720 9780 9840 9900 9960 10020 10080 10140 10200 10260 10320 10380 10440 10500 10560 10620 10680 10740 10800 10860 10920 10980 11040 11100 11160 11220 11280 11340 11400 11460 11520 11580 11640 11700 11760 11820 11880 11940 12000 12060 12120 12180 12240 12300 12360 12420 12480 12540 12600 12660 12720 12780 12840 12900 12960 13020 13080 13140 17 201142028 aaaggagcgt taattatggc gcttctgatg gcggcgcacg tctgtcatgc agctgttctt ccttcaggca gtcgctttga cccacgcaat cagatagtca gttataaccc caataatacc accataatta acagtgccgt tggatacacc accacactgg tatttgatga agatgaaaca gttatcagtg ccagaactgg ttttccgcag ggatgggcgg ttaataaaga agataacctg gtatacctgg aagttcgtcc tgttaaacag actgttcaga aaaataatat ggatgaaaac ggtaatacct cttctgaatc cgtcagtgtt gctcttgacc cggaaaatga gcttgaacgc tggcgaacga atttgtttgt tcgcaccacg aagcgtaatt acagcatgga gctgaacgcc cggacgttcc ggcagccgga gaaaattgcg tttgtggtga attaccagta tccgcaggaa cgccggaagg aacaggccga aattgagaag aaacgcacag aggctcttgc cagacgccag gaggagcagg caatcaaccg ttccctggaa aatgcgaaat cgccccgtaa ctggcagtac tggaagcggg ttgctgaagg cagccaggat atcagccctg attatgcata tgacgatggc cgttatacct ggttcggctt cagtccgtta aagaaaattc ccagcgtctt tgtgatgaac ggtatgcagg agactcttac caatcctgtg attaaacaga gcgggagttt tacggctgtt ggcgtaccgg ttgataagcg ttttgtttta cgtcttggtg agcaggtggt ggggattgag aaccagggct tcggaaaagt acgtttacca gccggagata cggtatcccc ggatgttaag aaagaggtga tccagtgact gaacaggaaa ataaaatccc gactgcaacc gaaattgaac agcagctacg ggaacgcaga cagaaagaac tggaacaggc cgggaagact ccggaagaag agcctggcaa gccagcattg cagcttggta ttgaaaaact taaaaagtca cgtaaaggga tgattatcct cgtcgtgggt tttcttctgc ttgctgccgg tgtttctgtt tattatatcc cgtccattat ccgttctgtg tcgtcagggg atgagaaacc cgeaagtcag ccgcttgcaa ccggaacggc taaacgtcag accggactga gcgaagatat cgatcctttt aataccgcac agaaaaaaac agagaaacca gaggaagaaa aagtcatttc ttctgaaaag actgaaccgc cggaaaataa acagcagagc ttcagccgtg cacttgacgt ttctcttgat ggcagccaga caggaaacag cagcagttca gcgggaacgt cagtttcaca tactgcggcc agtgagccag aaagcgataa aaaggatgaa gcaaaagcaa ccgcacagac tacagaatct gcgccactgg cgaaaataac gaaacttcca tatgacccaa atttgtttat cccggaaggg acatcaattc cctgttcact ggacaggcgt tttgtttctg acctggcggg gaaactggaa tgtacggtca acagcgatat atacagcgcc agcggtaatg taaaacttat cgaaagagga accgccgcaa aactgatgta taaggccggg tctttaaatc atggacaagg gcgtgtgttt gttatggctt acaagctacg tacccgcagt aagcctttta ttgatattcc cctggttgac tcacaggcgg ctggcgcgtt aggcgaagcc ggtgcttctg ggtggattga cactcatttc agtgaacgtt ttcttggtgc aatgatggtc gggatgatac cggatttaag tcaggccgcc agtggtattg cacagaacaa cagggacagc cagaccgact atacggcaaa cagtcgccag gcttttgctg atatagcacg cgaagcattt tctaatagtg tgaatattcc gccaacgctt tataaaaatc agggcgaaat tattactctg attgtcggtc aggatctgga tttttcaggc atttataaac tgaaaatgaa agggggttaa tgtgaataac gaaaacagac atctgattta tgatgtggtc aacgattatt tttatcactg gctgaatgag attgagggtg tcacggaaat tgctgttaac cgaccaggag aaatatttat aaaggtcagg ggaaagtggc aatggtatga acaaaagatg agttacagtg attgtctttc ttttgcatcc acactggccg attttcatga cggcggttct gtgactcctg aatatcccct gcgctctgcc acgcttccgg gtggagaacg tgttcaggtt gtgatcccac cggcaactga aaaagacact gtttctataa caatccgtaa gccgtcaggt atttttatca gtcatgacaa atttataaaa cagggatttt attcacgcgt cagtggttta agtggtgact cggttatgga agataatatt tctgctttaa tcacttccgg atattttgat cgggttatac ctgaatcact gcgtcagggg aaaacgatag ttttctgtgg agggacgggt tcaggtaaaa ctacctttgc aaatgcctgt ctggaatata taccgcatca tctgcggtgt atttctattg aagatactga tgaggcaaaa ttcagattcc ataaaaacca tgtaaaactt tactatccgg cagagggtga gagtaaggtt attacctcag cgagtcttct gcgttcctgt tttcgtatga atccggacag gattctgatg acagaaatca ggggggctga ggcatgggat tttctgaaag catcgagttc aggccatgca ggaaacatta ccaccgttca cgaaagtagt cctgaatatg ctgtgcttgg gattgttcag cgatgttata tgaatcctga atgtcagaat ctaccattca atgtcatttt aagacgtgta ctgagtaata ttgatattat catgagtatt aaataccttg atgatgaaga ttttcgtttc gcttccggta tttattacaa acaacttcat tttgatgact atttcagaaa actgaaggag tgattatgtc tttaaaactc cccgataaag gccagtgggt ttttatcggt ctggttatgt gtctcgtgac atattatact ggttctgttg ctgtttactt cctgaacgga aaaacgccgc tttatatatg gaaaaatttt gattccatgc tcctgtggcg aataataaca gagagtaata tacggacaga tatcaggtta accgctatcc cctctctttt atcaggtatg gtttcgtctc tcatcgtgcc tgtttttatt atctggcaac tgaataaaac ggctgttgct ctttatggtg acgcgaagtt tgccagtgat aatgatttaa ggaaatcgaa acttctgaaa tgggagaaag aaaacgatac tgatattctc gtcggagcat ataaaggtaa atacctgtgg tataccgcac cagattttgt atcacttggc gcaggaaccc 13200 13260 13320 13380 13440 13500 13560 13620 13680 13740 13800 13860 13920 13980 14040 14100 14160 14220 14280 14340 14400 14460 14520 14580 14640 14700 14760 14820 14880 14940 15000 15060 15120 15180 15240 15300 15360 15420 15480 15540 15600 15660 15720 15780 15840 15900 15960 16020 16080 16140 16200 16260 16320 16380 16440 16500 16560 16620 16680 16740201142028 tggatgagtt ctggcaatgg atcaataacg aagcgttcag ggactttgtt tacaacaagc tgaaaaccgg acgtaaactc gatatggtgc ttgtcgtagc cacacagtca ccggatgaac tgattaaatc acccattgcg gcagcggttc gtgagcaatg cgccactcat atctatctgg caaacccgaa agccaaacgt agtgaatatg ttgatggttt gcaggtcagg gagctttatt ttgacaaaat taaagctatc gatccgctgt cccgccagtt ccttgttgtt aagaacccac agaggaaagg tgaaagtgat gattttgctg cttttgccag actggagctg ggaaaagcag cgtattactt accggttctc agtgcatcaa aaccccagtt agaactgttc gatgaaatct ggaaagaagg aatgaagccg gaagagtggc ttgataccta tctggaacag gcgaacctga tttgaggaat caccaatgaa aaagcaaatt atggcggcat tcgtcgcttc actgattgtt atttccggcg ctcaggcagg gatacctgtt gccatcgacg ccaaccctga atgggcgatt gaagccggac gatggacaga acgccttaag caatgggcgg aaacggtaaa acattacgaa aatcagataa atgcgtacaa acaggagctg ctgtcaaaaa cgggtatccg tgatgtgcag ggactggtgc agtccgcaca gtcagtgagt caggaactga tgcagattta tgatcagggg aatgctttta ttgacgatta cattaaaaat cctgaagggg cgttatcgga acaggccaaa tcgttattgt cagattacaa agtaacggat acctgccaga acctgggata ttccggcgac ctggtacggg gatgtgaagc gacgttcctt tctcaactgg caagcgtgga atacggtaac aagctggaga gcaagcttcg tcaggacaac cagacgatga aagaccttat tgatcaagtc aaaaatgcga aggatacgaa ggccacgcag gatgcaacaa acgctgttgc acttgaacaa ctgaagttcg agaagctcaa atttcagtat caaatgtatc gcgataagca gcgagatctt gcagaatata aagagaagat ggctcaggca gctttcagaa aacagcaacg tgaagccgtg ccaccttctt acagaaaagc ttatatggca atgaaatcat atgaggatga ttaaatgaaa agtatcgcta ctgctggcct tgtcattata tctgttttta gtctttctgg atgttttgaa gaaacaaaat cggttgattg gtggcttgca catccgaagg agacatacaa aaaatttgaa gagtgtcaga agtctggtag cgattctgat aattgtaaga acgttaagcg agcacatttg tcttttgaac ggagaaaggc ggttggttta ccaataaatt gagggtgatg tatggggatt gtcactggta taccggatgg tactgattta tcaggtaatg taacctctcc cgcaaatgcg ggaggtgttt ctggatttga ttctgatttt tttcagacaa ctcatgaagt tatatttaat attctcaaca agagtatatc tggaaaatta agtgaatatt cagatgtggc ttatactctt ggtaaatatg gagtttctct gtatgtttta tggtatgctt ttactgtatt agcaaggaaa caacagacac ctgtacctga ttttatctgg aatatctgta ggttttacat aatattgctt tttgttaaga atacaggggg aatacttaca tcagcaacag atgctattga tggattgaaa aatacattgg cagggggaga tccgtgggta tggatggatc agttatgggt gaaggttata caagttgcaa ctcttatttt tgataaagat acatctactg tgcctgttgc cggtgggatt ggtgctttat taacttatgt cggtggtgtt ttggcattat tgctttgttc tatagtattt gcatctgctg aattaacatt actattactt tctgtcactg cgccaatatt tatcatgtgt ctgatgtttg gtttacttcg gcaaatgttt aatagctggc tacagcttaa ttttagctcg ttactggttt ttctatttgc agcattagca ctaagagctg ggacatggca attaaacatg gcattaagta cgtctattgc tacagcatca gaaaacaatc ttcttcaaac gggagtaact tcattagctg ctggcatttt catggcctgg attatctggc aggcgaaaag ttatgcttca cagattgcag gtgtgggtgt tgaaggtgcc atgcagggcg cagccgctat ggggattggt gctggcgttt tcggtgcatc ccgtatggcg cgtggcgcac ttggtatggg cagaaatgcc ggtattggtg catggaaagg tctgcgtcgt caggaagacg ggtttggtca gtctccggga ataacgggta agaccgctaa cctggccgga cagggcgtta atattggtgc caaaaagctt cgtcaggcag ctattgagag agcaaagaaa atgtatggtg ggtaatttaa ttatgagcgc cttttaatgt tcttttcata attgtcattg tcgggcctgg cgcgctctat tatcttttct gttcacactg agtcttctg t aaatattaat ctgttataca ggtgttcata ttatgaaact acttattgtg gctttcgtga cgctttgtct cgctggttgt caggcgtcac ataaactacc gcccgtttcc gggaaaagcg aacctgttaa ttctgctgag gtaatgcaaa atggaattta aacttcccgg atttaaaaat aaaaaagacg ttactgactc atcagtttca tttgaagaaa aaaacattgc actacaggag agaatgaatc gtatttataa attcggtggt atcggaggca tgttaattgg tgggctgtct ttacttgcat taaatgcagc attaccactg aaaacaacag ttgttgatgc ctaccttatc gataaggtta caggtgtggc tgaacgtctg acttctgtta aaaaagaaaa tctttctgaa aacgaagcca ttgcccgata ttttatcacc cagtatataa aacatcgtga aggttataat tttttcagtc tccagcatga ttatgattat gtaatggctt acagcgcgga gaatgtcgcg gcagattata acgcattatt taacagtgaa caggcaccaa aacttgttta taacaaagca gaaaaaacgg caatggttca ggataatcca tctgtcataa tttcaccttc gtcacgggca gatgataaag atatcggtgc gtatattcgt tttcgtctga ccatcaggga tgttgctacc ggacaaaccc gccaggagtt ctggaatgtt cgcctgactt atcgtatcga accgcaggtt gaaatggtgt caggggaacg taataacaat cctcttaaat tcgttgtaac aagctacgtt cgcgataaag aagccagagg ttaataatat gaaaatgaat 9600 9660 9720 9780 9840 9900 9960 10020 10080 10140 10200 10260 10320 10380 10440 10500 10560 10620 10680 10740 10800 10860 10920 10980 11040 11100 11160 11220 11280 11340 11400 11460 11520 11580 11640 11700 11760 11820 11880 11940 12000 12060 12120 12180 12240 12300 12360 12420 12480 12540 12600 12660 12720 12780 12840 12900 12960 13020 13080 13140 17 201142028 aaaggagcgt taattatggc gcttctgatg gcggcgcacg tctgtcatgc agctgttctt ccttcaggca gtcgctttga cccacgcaat cagatagtca gttataaccc caataatacc accataatta acagtgccgt tggatacacc accacactgg tatttgatga agatgaaaca gttatcagtg ccagaactgg ttttccgcag ggatgggcgg ttaataaaga agataacctg gtatacctgg aagttcgtcc tgttaaacag actgttcaga aaaataatat ggatgaaaac ggtaatacct cttctgaatc cgtcagtgtt gctcttgacc cggaaaatga gcttgaacgc tggcgaacga atttgtttgt tcgcaccacg aagcgtaatt acagcatgga gctgaacgcc cggacgttcc ggcagccgga Gaaaattgcg tttgtggtga attaccagta tccgcaggaa cgccggaaag aacaggccga aattgagaag aaacgcacag aggctcttcc cagacgccag gaggagcagg caatcaaccg ttccctggaa aatgcgaaat cgccccgtaa ctggcagtac tgga agcggg ttgctgaagg cagccaggat atcagccctg attatgcata tgacgatggc cgttatacct ggttcggctt cagtccgtta aagaaaattc ccagcgtctt tgtgatgaac ggtatgcagg agactcttac caatcctgtg attaaacaga gcgggagttt tacggctgtt ggcgtaccgg ttgataagcg ttttgtttta cgtcttggtg agcaggtggt ggggattgag aaccagggct tcggaaaagt acgtttacca gccggagata cggtatcccc ggatgttaag aaagaggtga tccagtgact gaacaggaaa ataaaatccc gactgcaacc gaaattgaac agcagctacg ggaacgcaga cagaaagaac tggaacaggc cgggaagact ccggaagaag agcctggcaa gccagcattg cagcttggta ttgaaaaact taaaaagtca cgtaaaggga tgattatcct cgtcgtgggt tttcttctgc ttgctgccgg tgtttctgtt tattatatcc cgtccattat ccgttctgtg tcgtcagggg atgagaaacc cgeaagtcag ccgcttgcaa ccggaacggc taaacgtcag accggactga gcgaagatat cgatcctttt aataccgcac agaaaaaaac agagaaacca gaggaagaaa aagtcatttc ttctgaaaag actgaaccgc cggaaaataa acagcagagc ttcagccgtg cacttgacgt ttctcttgat ggcagccaga caggaaacag cagcagttca gcgggaacgt cagtttcaca tactgcggcc agtgagccag aaagcgataa aaaggatgaa gcaaaagcaa ccgcacagac tacagaatct gcgccactgg cgaaaataac gaa acttcca tatgacccaa atttgtttat cccggaaggg acatcaattc cctgttcact ggacaggcgt tttgtttctg acctggcggg gaaactggaa tgtacggtca acagcgatat atacagcgcc agcggtaatg taaaacttat cgaaagagga accgccgcaa aactgatgta taaggccggg tctttaaatc atggacaagg gcgtgtgttt gttatggctt acaagctacg tacccgcagt aagcctttta ttgatattcc cctggttgac tcacaggcgg ctggcgcgtt aggcgaagcc ggtgcttctg ggtggattga cactcatttc agtgaacgtt ttcttggtgc aatgatggtc gggatgatac cggatttaag tcaggccgcc agtggtattg cacagaacaa cagggacagc cagaccgact atacggcaaa cagtcgccag gcttttgctg atatagcacg cgaagcattt tctaatagtg tgaatattcc gccaacgctt tataaaaatc agggcgaaat tattactctg attgtcggtc aggatctgga tttttcaggc atttataaac tgaaaatgaa agggggttaa tgtgaataac gaaaacagac atctgattta tgatgtggtc aacgattatt tttatcactg gctgaatgag attgagggtg tcacggaaat tgctgttaac cgaccaggag aaatatttat aaaggtcagg ggaaagtggc aatggtatga acaaaagatg agttacagtg attgtctttc ttttgcatcc acactggccg attttcatga cggcggttct gtgactcctg aatatcccct gcgctctgcc acgcttccgg gtggagaacg tgttcaggtt gtgatcccac cggcaactga aa aagacact gtttctataa caatccgtaa gccgtcaggt atttttatca gtcatgacaa atttataaaa cagggatttt attcacgcgt cagtggttta agtggtgact cggttatgga agataatatt tctgctttaa tcacttccgg atattttgat cgggttatac ctgaatcact gcgtcagggg aaaacgatag ttttctgtgg agggacgggt tcaggtaaaa ctacctttgc aaatgcctgt ctggaatata taccgcatca tctgcggtgt atttctattg aagatactga tgaggcaaaa ttcagattcc ataaaaacca tgtaaaactt tactatccgg cagagggtga gagtaaggtt attacctcag cgagtcttct gcgttcctgt tttcgtatga atccggacag gattctgatg acagaaatca ggggggctga ggcatgggat tttctgaaag catcgagttc aggccatgca ggaaacatta ccaccgttca cgaaagtagt cctgaatatg ctgtgcttgg gattgttcag cgatgttata tgaatcctga atgtcagaat ctaccattca atgtcatttt aagacgtgta ctgagtaata ttgatattat catgagtatt aaataccttg atgatgaaga ttttcgtttc gcttccggta tttattacaa acaacttcat tttgatgact atttcagaaa actgaaggag tgattatgtc tttaaaactc cccgataaag gccagtgggt ttttatcggt ctggttatgt gtctcgtgac atattatact ggttctgttg ctgtttactt cctgaacgga aaaacgccgc tttatatatg gaaaaatttt gattccatgc tcctgtggcg aataataaca gagagtaata t acggacaga tatcaggtta accgctatcc cctctctttt atcaggtatg gtttcgtctc tcatcgtgcc tgtttttatt atctggcaac tgaataaaac ggctgttgct ctttatggtg acgcgaagtt tgccagtgat aatgatttaa ggaaatcgaa acttctgaaa tgggagaaag aaaacgatac tgatattctc gtcggagcat ataaaggtaa atacctgtgg tataccgcac cagattttgt atcacttggc gcaggaaccc 13200 13260 13320 13380 13440 13500 13560 13620 13680 13740 13800 13860 13920 13980 14040 14100 14160 14220 14280 14340 14400 14460 14520 14580 14640 14700 14760 14820 14880 14940 15000 15060 15120 15180 15240 15300 15360 15420 15480 15540 15600 15660 15720 15780 15840 15900 15960 16020 16080 16140 16200 16260 16320 16380 16440 16500 16560 16620 16680 16740

1818

201142028 gcgcaggtaa aggtgccgcc attggtatcc ctaatcttct ggtcagaaaa cactctctga ttgcgttaga tccaaaacag gaattgtgga aaatcaccag taaggtgcgt gaaatactgc tgggtaataa agtttatctg ctcgaccctt tcaacagtaa aacacaccag tttaatcccc ttttctatat tgatttaaaa gcggagagtg gggctaagga tctgcttaaa ctgattgaaa ttctgtttcc gtcttatggc atgacagggg cagaagcgca ctttaataat cttgcgggtc aatactggac aggactggct aagttgcttc atttctttat taactatgag ccgtcctggc ttaatgagtt cgggcttaaa cccgttttct caatcggttc tgtcgtcgac ttgtacagca atattgaccg ggaactgata ctcagtaagc gggaagaact ggagggaaca aacgggcttg atgaaaacgc gttgtatcat ttgcgcgatg ccctgaccaa aatcagggaa tatcacgaaa cggaagatga acagcgttca agcattgatg gttctttccg taagaaaatg agcctgtttt atctcccaac cgttcgtaaa tgtactgatg gtaatgattt cgatctccgt cagttgcgac gggaagatat cactgtttat gtcggtgtta atgcggaaga tatatcactg gcttacgatt ttctgaacct gtttttcaac ttcgttgttg aagtgacatt gcgtgaaaat cctgattttg atcccaccct gaaacatgac tgcctgatgt ttcttgatga gttcccttcg attggttata tgccaattat taaaaaggga tcagggtata ttgcaggttt taaacttaaa ctgctgacaa tttatcagaa tatcagtcag ctaaatgaaa tctatggtat tgagggagcc aaaacgctga tgagtgctca tccctgccgt attatctatg ctgtcagcga agaggatgat gccgcgaaga tatcagaaaa acttgggtat attaccacta catcaaagag cacaagcaag aaccggggac gatcaacttc acagggcgaa tcagaaagtg aagcccgaag agcactggtg cttccacagg aactgggaac gctggacttt aaagaagagt ttatcatcct gaagggggag aaccctgtta aagcagaaaa ggcactttat taccttgatc cgtattttat ggacaggtta atgaaggtca gtcctaaact ggcatcattg acgatgaaac.taaataagac gaaaaaaata tttggtgtga aagggcttaa atatccgtca aaagaaaaaa tgctctccgt aggagagctg gagtctgagg ttttgctatg aaaaaaatac ttatgatcag tattcttgtc ctcacagcct gttcctctcc acctgaaccg ccacaggttg aatgggaaaa aaggcctgaa gttatgaata cacaaataat gaactggaaa ccaacatccg gtgttattaa atcaaataat ataaattcct catggagtaa ggtgttgcct gattttaaac cagaaaacca tctttacgat gattctgttt tttatgccgt tgcccattct gaaaaaatag ttgtaaggac atcttctttt gatagttact ggtcagcgaa agactggcta agaaaaaatg gtgcaacagg tgttattgaa tatcagccac taaaaagatg gttgaataat gactatgttg aaatttatct gtcaagaata aatattcaga ggttactatg aaaaggatat gcaaaggtct aatcgtaatt tttactgtgt cctgttttat agcgccaacg tatgccgctg atccctgtaa atccgttttt tgtctttatg gtaaagctgt cggtagtagt ggagggagtg agtgcagcag tgcagaaaag gattttttta aaaatgttga aaagaaaaag gggaaaatcc gctggggtaa aacatttgat ctccgtaaga attttttaaa tcagtgttca acggcagacc cggctgcaat ctcactgatc atgagtaagt ttggtcgcgt cagaggttga ttaaaacctg atacccgcgc caacgggtat caggggactg caagtatcac ataccgcgcc aacgaaatgt gatgattagt tacaatcact gaggttagaa atgaaagtaa ccaaatctat tatatctatt ttattgtgct tatctgctgg tagtgcataa gtggatgtaa taaaaaatgc tcgtaccttt ttgaaaggaa cccttgatct gagcgtgcag gaagcagatg aaagggaaga actttataag aagaacgggg cgcaacctga ctatttaagt tacctggagg attaacacat gcaatatgca ttatttgatg ggatggaacg aaagtttttg ctggatgctc ttgaatttgg tgttctgaag gactggaaag aaaatccggt aaaagaactt cctgatattg atgaatctgt tcaccccttc catgtctgtt atggtggata tttattaaac cctgatgttt cagatttaga tattagcaga aaaataaaag accagacagg attctggctg gcagctattg atgatacccg tatggattgt cattcaatag cttattatga tattcacacc ctccctttaa tttcgtgtgg tcatcagaag atagttcctt ttgcagcgtt aataaaagct gatgaatgca tcatttcaaa aattgcttcg tattctggtt ttgccgtaac agcctttttg agaattaaag accagaatat cgcaacgaat atacttaacc gtgagggaat ttttgccttt aatggctgtg agcacagatt cagacaacct gtaagtgaag ataactggca acaggcagtg tcagaagaac gcgctatccg ttgtgccaaa agattaattc aatgtaaagg ataacaaaat gagacttttt atcgctgaaa aacccgcagt agcaaatgat attgttaagg cacttggtgg caattttacc cgccatgatg gctggtttga aagtgataac gccattgtga ctaactgttt tggtcatatt atcgaatcac aaccgccgga aaactataat cctgaataca aagcctggaa ggttgaaacg cttcctttac gtctttatcc cgtgaagtat cagcctgttg aaagtgcagc aaaacaggtt aaaacgattc tcgaacttat cagacgtgga gacgtgactg aaattgttca cgctggcgat cctgatgatg agggacagct acttgttgat gaagtcctgg aatatgcagg aaacacaaaa cccgtaaagc gcgttctgat aaacgacaac acgcttccgg cagtgaaaaa ggcactggca aatcttaaag ataatcgtga tttcaaaggg ctttacctta aggcgctggc gcgttcagtt gccgatgccg tctatggatt ctccatgacg cgtgcttaca ccattcctgc aaaagccaga ggatatcagg gcgttctgtc tgtcgggcgc gtccagacac ccgttcttgg cctgattgtg aatcgtaccc 16800 16860 16920 16980 17040 17100 17160 17220 17280 17340 17400 17460 17520 17580 17640 17700 17760 17820 17880 17940 18000 18060 18120 18180 18240 18300 18360 18420 18480 18540 18600 18660 18720 18780 18840 18900 18960 19020 19080 19140 19200 19260 19320 19380 19440 19500 19560 19620 19680 19740 19800 19860 19920 19980 20040 20100 20160 20220 20280 20340 19 201142028201142028 gcgcaggtaa aggtgccgcc attggtatcc ctaatcttct ggtcagaaaa cactctctga ttgcgttaga tccaaaacag gaattgtgga aaatcaccag taaggtgcgt gaaatactgc tgggtaataa agtttatctg ctcgaccctt tcaacagtaa aacacaccag tttaatcccc ttttctatat tgatttaaaa gcggagagtg gggctaagga tctgcttaaa ctgattgaaa ttctgtttcc gtcttatggc atgacagggg cagaagcgca ctttaataat cttgcgggtc aatactggac aggactggct aagttgcttc atttctttat taactatgag ccgtcctggc ttaatgagtt cgggcttaaa cccgttttct caatcggttc tgtcgtcgac ttgtacagca atattgaccg ggaactgata ctcagtaagc gggaagaact ggagggaaca aacgggcttg atgaaaacgc gttgtatcat ttgcgcgatg ccctgaccaa aatcagggaa tatcacgaaa cggaagatga acagcgttca agcattgatg gttctttccg taagaaaatg agcctgtttt atctcccaac cgttcgtaaa tgtactgatg gtaatgattt cgatctccgt cagttgcgac gggaagatat cactgtttat gtcggtgtta atgcggaaga tatatcactg gcttacgatt ttctgaacct gtttttcaac ttcgttgttg aagtgacatt gcgtgaaaat cctgattttg atcccaccct gaaacatgac tgcctgatgt ttcttgatga gttcccttcg attggttata tgccaattat taaaaaggga tcagggtata ttgcaggttt taaacttaaa ctgctgacaa tttatcagaa tatcagtcag ctaaatgaaa tctatggtat aaaacgctga tgagtgctca tccctgccgt attatctatg ctgtcagcga agaggatgat gccgcgaaga tatcagaaaa acttgggtat attaccacta catcaaagag cacaagcaag aaccggggac gatcaacttc acagggcgaa tcagaaagtg aagcccgaag agcactggtg cttccacagg aactgggaac gctggacttt aaagaagagt ttatcatcct gaagggggag aaccctgtta aagcagaaaa ggcactttat taccttgatc cgtattttat ggacaggtta atgaaggtca gtcctaaact ggcatcattg acgatgaaac.taaataagac gaaaaaaata tttggtgtga aagggcttaa atatccgtca aaagaaaaaa tgctctccgt aggagagctg gagtctgagg ttttgctatg tgagggagcc aaaaaaatac ttatgatcag tattcttgtc ctcacagcct gttcctctcc acctgaaccg ccacaggttg aatgggaaaa aaggcctgaa gttatgaata cacaaataat gaactggaaa ccaacatccg gtgttattaa atcaaataat ataaattcct catggagtaa ggtgttgcct gattttaaac cagaaaacca tctttacgat gattctgttt tttatgccgt tgcccattct gaaaaaatag ttgtaaggac atcttctttt gatagttact ggtcagcgaa agactggcta agaaaaaatg gtgcaacagg tgttattgaa tatcagccac taaaaagatg gttgaataat gactatgttg aaatttatct gtcaagaata aatattcaga ggttactatg aaaaggatat gcaaaggtct aatcgtaatt tttactgtgt cctgttttat agcgccaacg tatgccgctg atccctgtaa atccgttttt tgtctttatg gtaaagctgt cggtagtagt ggagggagtg agtgcagcag tgcagaaaag gattttttta aaaatgttga aaagaaaaag gggaaaatcc gctggggtaa aacatttgat ctccgtaaga attttttaaa tcagtgttca acggcagacc cggctgcaat ctcactgatc atgagtaagt ttggtcgcgt cagaggttga ttaaaacctg atacccgcgc caacgggtat caggggactg caagtatcac ataccgcgcc aacgaaatgt gatgattagt tacaatcact gaggttagaa atgaaagtaa ccaaatctat tatatctatt ttattgtgct tatctgctgg tagtgcataa gtggatgtaa taaaaaatgc tcgtaccttt ttgaaaggaa cccttgatct gagcgtgcag gaagcagatg aaagggaaga actttataag aagaacgggg cgcaacctga ctatttaagt tacctggagg attaacacat gcaatatgca ttatttgatg ggatggaacg aaagtttttg ctggatgctc ttgaatttgg tgttctgaag gactggaaag aaaatccggt aaaagaactt cctgatattg atgaatctgt tcaccccttc catgtctgtt atggtggata tttattaaac cctgatgttt cagatttaga tattagcaga aaaataaaag accagacagg attctggctg gcagctattg atgatacccg tatggattgt cattcaatag cttattatga tattcacacc ctccctttaa tttcgtgtgg tcatcagaag atagttcct t ttgcagcgtt aataaaagct gatgaatgca tcatttcaaa aattgcttcg tattctggtt ttgccgtaac agcctttttg agaattaaag accagaatat cgcaacgaat atacttaacc gtgagggaat ttttgccttt aatggctgtg agcacagatt cagacaacct gtaagtgaag ataactggca acaggcagtg tcagaagaac gcgctatccg ttgtgccaaa agattaattc aatgtaaagg ataacaaaat gagacttttt atcgctgaaa aacccgcagt agcaaatgat attgttaagg cacttggtgg caattttacc cgccatgatg gctggtttga aagtgataac gccattgtga ctaactgttt tggtcatatt atcgaatcac aaccgccgga aaactataat cctgaataca aagcctggaa ggttgaaacg cttcctttac gtctttatcc cgtgaagtat cagcctgttg aaagtgcagc aaaacaggtt aaaacgattc tcgaacttat cagacgtgga gacgtgactg aaattgttca cgctggcgat cctgatgatg agggacagct acttgttgat gaagtcctgg aatatgcagg aaacacaaaa cccgtaaagc gcgttctgat aaacgacaac acgcttccgg cagtgaaaaa ggcactggca aatcttaaag ataatcgtga tttcaaaggg ctttacctta aggcgctggc gcgttcagtt gccgatgccg tctatggatt ctccatgacg cgtgcttaca ccattcctgc aaaagccaga ggatatcagg gcgttctgtc tgtcgggcgc gtccagacac ccgttcttgg cctgattgtg aatcgtaccc 16800 16860 16920 16980 17040 17100 17160 17220 17280 17340 17400 17460 17520 17580 17640 17700 17760 17820 17880 17940 18000 18060 18120 18180 18240 18300 18360 18420 18480 18540 18600 18660 18720 18780 18840 18900 18960 19020 19080 19140 19200 19260 19320 19380 19440 19500 19560 19620 19680 19740 19800 19860 19920 19980 20040 20100 20160 20220 20280 20340 19 201142028

gtgctaacca gaaccataaa tccagttttt actacaccat gaccggagtt tttcagcgtg 20400 gtgctgatgt tctcagggcg aactggaaac caggtgaatt tgctccgctg accgaccgta 20460 aattacttga taaggcgtgg gcagacggaa cggcagcatc ccttgcagga aaaccggcta 20520 cagttgaagc agcagcaact gatgataaaa aaacggctgc gccgttgccg tttaacctgg 20580 tcagactcca gcaatacatg aacaagaagt ttaaaatgac ggcacaaaaa acgctggata 20640 ttacgcaaca actacgtgaa aaatataaag caattactta taaccgctca gattgctcat 20700 atctttctga tgaacaattc agtgaagcgc cgcaggttat cgatgccctg aaatcagtct 20760 ttcctcagtc gctggatatt gattcttcac gtaaaagcaa agcgtttaac agtgcaaagg 20820 tgactgcgca tactgcgata atcccgacct ccagtgtgcc tgatgttaac gcactcagca 20880 ccgacgageg caatgtttac ctggcgatcg cacaacacta tcttgttcag ttcatgcctg 20940 aaaaagcata ccaggaagta tcggttgcca ttcagtgtgg tgatgagtcg ttctatgccc 21000 gtgccagaaa aacaactgac agcggatttg aggcgtttct tggcgcggaa atcacagacg 21060 aaggtgaatc agaagataat gatgattccg cttttgaact gctctgtaaa attcgcacag 21120 gagaaacact gacgacaaaa gaagttgttg ttaatgagaa gaaaacaaca ccgctgccgt 21180 tattcaccga agcctccttg cttgctgcgc ttgttcgtgt cgcggatttt gtcactgatc 21240 caacgattaa aaaattactg aaagataagg ataaagacaa aaaagatgaa catggcggta 21300 ttggtacgcc agctacccgt gcagccattc tggaaacgct gaagaagaga aactatatca 21360 cgctggaaaa agggaaactt attccgactg ataccggata tgcgcttatt gatgccctgc 21420 caggtatagc ggttaatcct gatatgacag cattatggtc tgaaaagcag actgccattg 21480 aaaatggcga cctgacggtt gaacagttta ttaatgagct gtacggtgaa ttgacaggca 21540 tgatttctga tgttgacctg ggcaagatga agattgaacc cgctgcgcca gcagggcagt 21600 ttcaacgcct ggactctccc tgcccttcct gtggtaaaca tattgttatc aggccgaaag 21660 gttatttctg taccggatgt gaatttaaaa tctggagtga gttttctggt aagaaaatca 21720 cccaggcaca ggccgaaaaa ctggttaaat cagggaaaac cgatttgatt aagggattta 21780 aaaagaaaag tggtggaacg tatgatacag ttcttgtcct tgaggataag aaaacaggga 21840 agctgggttt tccggcaagg gctaagaagt gaaaacaaag caggaatggc tttttcagtt 21900 aagaaaatgt acatcaagag atactcttga aaaagttatt gagattaacc gttacaagct 21960 gcctttatca gaatcagagg cattttattc tgccgcagat caccgccgtg cagaactggt 22020 gatgaataaa ctttatgata aggttccttc cggcgtatgg aagtacgtcc attaaacaag 22080 aggattaatt atgagcgaac tgactaaaga agatgaatac ggcattatca gccggactat 22140 gatgaatatt cgttcattgc gtgtgtttgc ccgtgagatt gattttgagc agttgctcga 22200 aatgcaggaa aagctcaacg ttgttattga agaacgtcgt gaagatgctg aacgtgaagc 22260 ggctgaacga gcagagcgtg aacggaaacg tcaggaactg cttcagttaa tcgccggaga 22320 gggtttctca ccggaagaac tgcttggtct gtccgaagaa gcaccaaaat cacgtaaaaa 22380 aacgttacca aaagcgccac ccaagtatca atttgaagaa aatggtgaaa cgaaatactg 22440 gtctggtcgt ggacgtgcgc caaaaccgat tgatgaagcg ttgaaagccg ggcgttctct 22500 ggaagatttt cgtatcaata agagtttgaa cggagtaaca gatgagcagt aatatggcaa 22560 ggatatagtt ttatatcatc attttgttaa ggaagaaaat ccatgagtaa tacatcctac 22620 aaacaaatta tccctgcgac agactggtat ttccgtcacg ataatgtctc cggtgtggca 22680 ggaaagtcaa cagtatacca actggctgca tgggcgctta aagaaaatgg tgaggtagtt 22740 ggtctggtga cggttcgtga tgataatggg cgtcctaaac tggttactcc tccccctgtc 22800 cctggtgatt atttgcataa agaacaactc accgatgatg aaaaagagtg ggcgaagaga 22860 cgctaaacta tattcatata aagcctctgt tctagaggct ttattagcat gtttagttgc 22920 actggagttg ataatggctt caatgctcaa attgaggtaa attgatattg attttacttt 22980 tccgttgctc gtttctcgtc ttattatatt taaatgacaa ttgtaattgt ataatgtttt 23040 attactcttg aattgtctga gcctgatatt tttcaataat aatgttattg attcctaaat 23100 ctgatagcag ataagatgtt acaattgttt ttaggtagtt tccaatttta acataatcac 23160 caatcgtcaa tatgttcatt aactcctttt tgcggtcaac atgtgctaat tcatttctga 23220 gtgtggcaat attttcacca atagatttat tgttgaattt cttaaaaaac atctctatct 23280 cttgaataag aaacaaactt gcatattcat taatgggttt catgtatttt tcattttttg 23340 agccacctat tgttttgttt attgcttcta attgagtcgc aaacaatata atatcagtat 23400 gagcttgatg cagtgttcga aaaccggttt catactgata tgttattgtc aatggcatat 23460 atctttctgc tattttgaac catttgcaga atatctttcc aagattaata tgcttcctgt 23520 ttattggaag gagttgatgt tttatttccc tcagcgctag gtcaatagtt ctttgttcaa 23580 accccgttgt taagagacat ggtgtcttcg aatcatttcc cttgaacttt atgtttattt 23640 cttcaggaag agtcggtttg ttcagaagaa ttgaaaataa cccggaaata tcccagcact 23700 ttgatatatg gtcttttatt cctaaatcat tagatgattt tatcctaaag tagaaaacaa 23760 gttcttttct aatggagaaa aaagcatcag ggtataactc ttttgtcttt tttagttgat 23820 gaattatgtt ctccagtgcg gctttgtttt ggcaatttat tatgtttaat agatcatcac 23880 caattacgct aaaagataca tggttgacaa gttgaagagt ccaatggttt ccttttgcga 23940 20gtgctaacca gaaccataaa tccagttttt actacaccat gaccggagtt tttcagcgtg 20400 gtgctgatgt tctcagggcg aactggaaac caggtgaatt tgctccgctg accgaccgta 20460 aattacttga taaggcgtgg gcagacggaa cggcagcatc ccttgcagga aaaccggcta 20520 cagttgaagc agcagcaact gatgataaaa aaacggctgc gccgttgccg tttaacctgg 20580 tcagactcca gcaatacatg aacaagaagt ttaaaatgac ggcacaaaaa acgctggata 20640 ttacgcaaca actacgtgaa aaatataaag caattactta taaccgctca gattgctcat 20700 atctttctga tgaacaattc agtgaagcgc cgcaggttat cgatgccctg aaatcagtct 20760 ttcctcagtc gctggatatt gattcttcac gtaaaagcaa agcgtttaac agtgcaaagg 20820 tgactgcgca tactgcgata atcccgacct ccagtgtgcc tgatgttaac gcactcagca 20880 ccgacgageg caatgtttac ctggcgatcg cacaacacta tcttgttcag ttcatgcctg 20940 aaaaagcata ccaggaagta tcggttgcca ttcagtgtgg tgatgagtcg ttctatgccc 21000 gtgccagaaa aacaactgac agcggatttg aggcgtttct tggcgcggaa atcacagacg 21060 aaggtgaatc agaagataat gatgattccg cttttgaact gctctgtaaa attcgcacag 21120 gagaaacact gacgacaaaa gaagttgttg ttaatgagaa gaaaacaaca ccgctgccg t 21180 tattcaccga agcctccttg cttgctgcgc ttgttcgtgt 21240 cgcggatttt gtcactgatc tgttgacctg ggcaagatga agattgaacc caacgattaa aaaattactg aaagataagg ataaagacaa aaaagatgaa catggcggta 21300 ttggtacgcc agctacccgt gcagccattc tggaaacgct gaagaagaga aactatatca 21360 cgctggaaaa agggaaactt attccgactg ataccggata tgcgcttatt gatgccctgc 21420 caggtatagc ggttaatcct gatatgacag cattatggtc tgaaaagcag actgccattg 21480 aaaatggcga cctgacggtt gaacagttta ttaatgagct gtacggtgaa ttgacaggca 21540 tgatttctga cgctgcgcca gcagggcagt 21600 ttcaacgcct ggactctccc tgcccttcct gtggtaaaca tattgttatc aggccgaaag 21660 gttatttctg taccggatgt gaatttaaaa tctggagtga gttttctggt aagaaaatca 21720 cccaggcaca ggccgaaaaa ctggttaaat cagggaaaac cgatttgatt aagggattta 21780 aaaagaaaag tggtggaacg tatgatacag ttcttgtcct tgaggataag aaaacaggga 21840 agctgggttt tccggcaagg gctaagaagt gaaaacaaag caggaatggc tttttcagtt 21900 aagaaaatgt acatcaagag atactcttga aaaagttatt gagattaacc gttacaagct 21960 gcctttatca gaatcagagg cattttattc tgccgcagat caccgccgtg c agaactggt 22020 gatgaataaa ctttatgata aggttccttc cggcgtatgg aagtacgtcc attaaacaag 22080 aggattaatt atgagcgaac tgactaaaga agttgctcga agatgaatac ggcattatca gccggactat 22140 gatgaatatt cgttcattgc gtgtgtttgc ccgtgagatt gattttgagc aaagcgccac ccaagtatca atttgaagaa 22200 aatgcaggaa aagctcaacg ttgttattga agaacgtcgt gaagatgctg aacgtgaagc 22260 ggctgaacga gcagagcgtg aacggaaacg tcaggaactg cttcagttaa tcgccggaga 22320 gggtttctca ccggaagaac tgcttggtct gtccgaagaa gcaccaaaat cacgtaaaaa 22380 aacgttacca aatggtgaaa cgaaatactg 22440 gtctggtcgt ggacgtgcgc caaaaccgat tgatgaagcg ttgaaagccg ggcgttctct 22500 ggaagatttt cgtatcaata agagtttgaa cggagtaaca gatgagcagt aatatggcaa 22560 ggatatagtt ttatatcatc attttgttaa ggaagaaaat ccatgagtaa tacatcctac 22620 aaacaaatta tccctgcgac agactggtat ttccgtcacg ataatgtctc cggtgtggca 22680 ggaaagtcaa cagtatacca actggctgca tgggcgctta aagaaaatgg tgaggtagtt 22740 ggtctggtga cggttcgtga tgataatggg cgtcctaaac tggttactcc tccccctgtc 22800 cctggtgatt atttgcataa agaacaactc accgatgatg aaaa agagtg ggcgaagaga 22860 cgctaaacta tattcatata aagcctctgt tctagaggct ttattagcat gtttagttgc 22920 actggagttg ataatggctt caatgctcaa attgaggtaa attgatattg attttacttt 22980 tccgttgctc gtttctcgtc ttattatatt taaatgacaa ttgtaattgt ataatgtttt 23040 attactcttg aattgtctga gcctgatatt tttcaataat aatgttattg attcctaaat 23100 ctgatagcag ataagatgtt acaattgttt ttaggtagtt tccaatttta acataatcac 23160 caatcgtcaa tatgttcatt aactcctttt tgcggtcaac atgtgctaat tcatttctga 23220 gtgtggcaat attttcacca atagatttat tgttgaattt cttaaaaaac atctctatct 23280 cttgaataag aaacaaactt gcatattcat taatgggttt catgtatttt tcattttttg 23340 agccacctat tgttttgttt attgcttcta attgagtcgc aaacaatata atatcagtat 23400 gagcttgatg cagtgttcga aaaccggttt catactgata tgttattgtc aatggcatat 23460 atctttctgc tattttgaac catttgcaga atatctttcc aagattaata tgcttcctgt 23520 ttattggaag gagttgatgt tttatttccc tcagcgctag gtcaatagtt ctttgttcaa 23580 accccgttgt taagagacat ggtgtcttcg aatcatttcc cttgaacttt atgtttattt 23640 cttcaggaag agtcggtttg ttcagaagaa ttgaaaa taa cccggaaata tcccagcact 23700 ttgatatatg gtcttttatt cctaaatcat tagatgattt tatcctaaag tagaaaacaa 23760 gttcttttct aatggagaaa aaagcatcag ggtataactc ttttgtcttt tttagttgat 23820 gaattatgtt ctccagtgcg gctttgtttt ggcaatttat tatgtttaat agatcatcac 23880 caattacgct aaaagataca tggttgacaa gttgaagagt ccaatggttt ccttttgcga 23940 20

201142028 taaatattgg atgttctaaa tgtttcaatt gagtgaaaaa accgtgaggg tggataaatt cttgcaatcc gtgcaaggat aaatcacaat attctatctt tgagtcgggg gcataaaaat cattgaatag cattatggga aatccatgcc ttccagtatg gataatccct ttaccaaaat gaatattccc ttgtgtgaaa tcaaatttcc caattagagt acatctttct ccagtattta agactccata taatatttca catgttctgg ggctttcact gtcagaaata caataatcta gaatcagacc atggtacggt gaatattcaa ttcgtgcaga aaatcttcct ttgttatcat caagttggtg ccaaaattca ccatgaaaaa agtattcttt tgttagttcg tatttttttg ttgccatgta ctgctccatt tgtttctgag aatccttcag aatagtaaaa atactactca aaactcgtct tcaagatctc tggctatctg ctcattttca tgatgctcaa cagcatcact gatcaaattc atcatgttat taagaaactc ctctacaact ttaaacgcat cattgagcgt agctgtattg aatgtatgta tcttttcatc cgttgttttc gttttcccgt tacgatgaac tatgtcatgc ctcagtttca tcaattcatt gatatttttg agtggaaaac gtggatattg cttaggttgc aggactgctt tatatatttc aactaccagt tggattctat gataaagaat atctgaaagg tactcctgta catatttgtt tgcattagac tctttattta tcaactcaga cagagagata ttctttgctt tcagttcatt tatgtttctt atggcatttt ctacatatct gttatgtgac aatacaacgc ttttaatcat ttcgctaaga cagttttcca ttattgtcac aatgtatgcg attttcattt ttatgaaaac ttcacttgta gattgttttc cctcttcttt tatttgttct aaaaggttta ttgctatttt ataaatttct gtatgtggat gctgttttaa ccatttttcg ttttcatatt cttccatagc catatcagaa agaaagtcct gatactcgtc atagtccttt tcaagtaact gccattcttc agaatcctcc tcaagatctg ggctggaaag ccgttctcgt atccattcct cacggcgact ttcctcgact tgcataaccc aatcttttgt agctcccaca ataccctcct tatgttcatt gctaaatatc aatagtcact ttatcggaga ctacgtgaaa aatcttctcc tttccccctg cttttttatc agatttcacg cagcgtaccg ccgctactgt atccatcaaa aaaactgata gctttttaat catcttttgt gctgcaactt actgaaaaaa ggattaatgg aaacgccgat aacggcattg atctgttggc caacgcgacc ggcagcattg gacttaacaa aaaaactgaa agaaaatccc ccaaatgaag ggaaaaaacg gaagaacagt cgaacgacag gcacaaaaaa acccgactgg taatcgggct ttttcgtgtt ttccagtcct gagttggtgg ctctgactgg aggaagatac tctacatcca acatccacaa tattatggca ctagacacca gttgtctagt ttgacttcag attgtctgtc aatggccatt tctttaaatg aattttatgc gtgcatctgt ataatctttg tgttggcgct gtacagtgtg cctcccttgc ttctaacccc tgcgctatct ggggatgaac gaattaaagt cctggctgaa caaacaggtg gtgctgtgtt cagtgctctg gcagggggaa ggtactctac atccaacaat gtgagagagc cgttgtgaca ggcgatgtat gagcaagcaa cggtgacttc ctccagtatc aacctagaga aacggatgat gaatctgtta atcatcgttc tcagggctgt gctcgccatt gcaaacgcgc tgattgccat tcagaaacta atcgagtgat tgatggctta actaagggag agacgtttac cgcttcctgc cctttaacaa aggagtgttt atgagttttg aacaattatt tacactaacg acccaacgtc gttgggtttg ccgcagaaaa tttattcatt taaaatagat ctaaatgaag cgggaaactg cagacgtaaa aaaaccgact ggtgaggtcg gcttttttac agttccggta cgagctggta actcgcccgg aagagaaact ctctacaact gacaacagta tgataatccg tgaagcctga ttatgcaata tgacaggcgg tgactacgcg ttaacggttt gcttgaaagt tagataaacg tataatcaga gggttagtgc tgtggtgagt ctttccctgc attccgctaa atgcggcatg aacagcttca aagtcctgac aggtaataca ggtggtgctg gttatctgtg ctctggtagg agtgagactc tctacaactg acaagacgaa gagaagccgt ggtgacaggc agcgtaagag caagccacgg tgtctttact ccatttatgg agaaaacgga tgattaacct gttaatcatc gttctgaggg cggtagctgc gcttgcaaac gcgctgattg cagttctgga actgatccgt cagttcatcg attgatgact gcggaaacgt aactaagggc aggaagtgca ccgcttcctg ccctttaaca aaggaggtgt tatggatttt atgagcattt tggggcgctg tttacttctt cctttccctg ttgcgatgtt tgtcggttgc ctgtttccag gcatcgatga tcgttttact ggaatgttca tgagttttat tgtcggttgt ctatcgtggt atatcaccaa gccaaaaccg caaaaggctg aggtcgcgta agcggccttt tattttgata cgcattagtt acaacattca atttgctttt taaaaatatc cacttcagca cctccctcat aaataacgtc aaccagttta ttgagctcac cctaccctac tcaccggagg attgcaggga tttacgtttt attgcgctac tgattgtgcc gcggctgcac cctagtacct tctgtacctg tgtccaggag ctgccacttt ctatcaacct gataattgca tcatgtctgg cttgattagg tttacgccct ttatatttgc cgtctttcct tgctttctct atcccctgtt tctggcgttc ccgccgttgt tcatagtccc ttctagcaac agcggccagc atatccagca gcatatcgtt aatggctgca aacaaccggc tgtcaaactc actcatacca gaattaatcc aggtcgtcgg cacattcacg gccataaccc ggatatcttt ctgacggatc attttcttca gcgtattcca gtcttcccct gacagtcggg aaagtctgtc cacatcctcg ataagcaaaa tgtcattttg ctggcaatct ctcagaagac ggaagagttc cgggcggtca agacgggagc cggattcatt 24000 24060 24120 24180 24240 24300 24360 24420 24480 24540 24600 24660 24720 24780 24840 24900 24960 25020 25080 25140 25200 25260 25320 25380 25440 25500 25560 25620 25680 25740 25800 25860 25920 25980 26040 26100 26160 26220 26280 26340 26400 26460 26520 26580 26640 26700 26760 26820 26880 26940 27000 27060 27120 27180 27240 27300 27360 27420 27480 27540 21 201142028201142028 taaatattgg atgttctaaa tgtttcaatt gagtgaaaaa accgtgaggg tggataaatt cttgcaatcc gtgcaaggat aaatcacaat attctatctt tgagtcgggg gcataaaaat cattgaatag cattatggga aatccatgcc ttccagtatg gataatccct ttaccaaaat gaatattccc ttgtgtgaaa tcaaatttcc caattagagt acatctttct ccagtattta agactccata taatatttca catgttctgg ggctttcact gtcagaaata caataatcta gaatcagacc atggtacggt gaatattcaa ttcgtgcaga aaatcttcct ttgttatcat caagttggtg ccaaaattca ccatgaaaaa agtattcttt tgttagttcg tatttttttg ttgccatgta ctgctccatt tgtttctgag aatccttcag aatagtaaaa atactactca aaactcgtct tcaagatctc tggctatctg ctcattttca tgatgctcaa cagcatcact gatcaaattc atcatgttat taagaaactc ctctacaact ttaaacgcat cattgagcgt agctgtattg aatgtatgta tcttttcatc cgttgttttc gttttcccgt tacgatgaac tatgtcatgc ctcagtttca tcaattcatt gatatttttg agtggaaaac gtggatattg cttaggttgc aggactgctt tatatatttc aactaccagt tggattctat gataaagaat atctgaaagg tactcctgta catatttgtt tgcattagac tctttattta tcaactcaga cagagagata ttctttgctt tcagttcatt tatgtttctt atggcatttt ctacatatct gttatgtgac aatacaacgc ttttaatcat ttcgctaaga cagttttcca ttattgtcac aatgtatgcg attttcattt ttatgaaaac ttcacttgta gattgttttc cctcttcttt tatttgttct aaaaggttta ttgctatttt ataaatttct gtatgtggat gctgttttaa ccatttttcg ttttcatatt cttccatagc catatcagaa agaaagtcct gatactcgtc atagtccttt tcaagtaact gccattcttc agaatcctcc tcaagatctg ggctggaaag ccgttctcgt atccattcct cacggcgact ttcctcgact tgcataaccc aatcttttgt agctcccaca ataccctcct tatgttcatt gctaaatatc aatagtcact ttatcggaga ctacgtgaaa aatcttctcc tttccccctg cttttttatc agatttcacg cagcgtaccg ccgctactgt atccatcaaa aaaactgata gctttttaat catcttttgt gctgcaactt actgaaaaaa ggattaatgg aaacgccgat aacggcattg atctgttggc caacgcgacc ggcagcattg gacttaacaa aaaaactgaa agaaaatccc ccaaatgaag ggaaaaaacg gaagaacagt cgaacgacag gcacaaaaaa acccgactgg taatcgggct ttttcgtgtt ttccagtcct gagttggtgg ctctgactgg aggaagatac tctacatcca acatccacaa tattatggca ctagacacca gttgtctagt ttgacttcag attgtctgtc aatggccatt tctttaaatg aattttatgc gtgcatctgt ataatctttg tgttggcgct gtacagtgtg cctcccttgc ttctaacccc tgcgctatct ggggatgaac gaattaaagt cctggctgaa caaacaggtg gtgctgtgtt cagtgctctg gcagggggaa ggtactctac atccaacaat gtgagagagc cgttgtgaca ggcgatgtat gagcaagcaa cggtgacttc ctccagtatc aacctagaga aacggatgat gaatctgtta atcatcgttc tcagggctgt gctcgccatt gcaaacgcgc tgattgccat tcagaaacta atcgagtgat tgatggctta actaagggag agacgtttac cgcttcctgc cctttaacaa aggagtgttt atgagttttg aacaattatt tacactaacg acccaacgtc gttgggtttg ccgcagaaaa tttattcatt taaaatagat ctaaatgaag cgggaaactg cagacgtaaa aaaaccgact ggtgaggtcg gcttttttac agttccggta cgagctggta actcgcccgg aagagaaact ctctacaact gacaacagta tgataatccg tgaagcctga ttatgcaata tgacaggcgg tgactacgcg ttaacggttt gcttgaaagt tagataaacg tataatcaga gggttagtgc tgtggtgagt ctttccctgc attccgctaa atgcggcatg aacagcttca aagtcctgac aggtaataca ggtggtgctg gttatctgtg ctctggtagg agtgagactc tctacaactg acaagacgaa gagaagccgt ggtgacaggc agcgtaagag caagccacgg tgtctttact ccatttatgg agaaaacgga tgattaacct gttaatcatc gttctgaggg cggtagctgc gcttgcaaac gcgctgattg cagttctgga actgatccg t cagttcatcg attgatgact gcggaaacgt aactaagggc aggaagtgca ccgcttcctg ccctttaaca aaggaggtgt tatggatttt atgagcattt tggggcgctg tttacttctt cctttccctg ttgcgatgtt tgtcggttgc ctgtttccag gcatcgatga tcgttttact ggaatgttca tgagttttat tgtcggttgt ctatcgtggt atatcaccaa gccaaaaccg caaaaggctg aggtcgcgta agcggccttt tattttgata cgcattagtt acaacattca atttgctttt taaaaatatc cacttcagca cctccctcat aaataacgtc aaccagttta ttgagctcac cctaccctac tcaccggagg attgcaggga tttacgtttt attgcgctac tgattgtgcc gcggctgcac cctagtacct tctgtacctg tgtccaggag ctgccacttt ctatcaacct gataattgca tcatgtctgg cttgattagg tttacgccct ttatatttgc cgtctttcct tgctttctct atcccctgtt tctggcgttc ccgccgttgt tcatagtccc ttctagcaac agcggccagc atatccagca gcatatcgtt aatggctgca aacaaccggc tgtcaaactc actcatacca gaattaatcc aggtcgtcgg cacattcacg gccataaccc ggatatcttt ctgacggatc attttcttca gcgtattcca gtcttcccct gacagtcggg aaagtctgtc cacatcctcg ataagcaaaa tgtcattttg ctggcaatct ctcagaagac ggaagagttc cgggcggtca agacgggagc cggattcatt 24000 24060 24120 24180 24240 24300 24360 24420 24480 24540 24600 24660 24720 24780 24840 24900 24960 25020 25080 25140 25200 25260 25320 25380 25440 25500 25560 25620 25680 25740 25800 25860 25920 25980 26040 26100 26160 26220 26280 26340 26400 26460 26520 26580 26640 26700 26760 26820 26880 26940 27000 27060 27120 27180 27240 27300 27360 27420 27480 27540 21 201142028

ctcaatataa tagctgcaaa tgatcaggcc tctttccctg gcaaaagcct cgatagtttc 27600 cagggcacgc gaagcatcct gttctgctgt tgaagctctc agataagcgc ggacaaagtt 27660 tgttggtgca tttgaagtca tattacagaa ccagttcgca taaggtcaca ccactatacc 27720 caaaatgaac cagaaagtgg atcgacagga aaggcacact ctaaaagaac cgaaaaaaca 27780 aataatgagt aaatgtgaca tcgtcaccca tactcataaa ctcacatgct catatacaca 27840 acaaactcaa atgagtatgt gctctttaac tcataaactc atattaacct ttactcatgc 27900 aaagagtatg gtaaactcaa aaactcacaa gagtaattga gtagatacac aattgagggt 27960 ttgagtatga aagtaatctc atttctgaat ccgaaagggg gttcaggtaa aacaactgcc 28020 gtaatcaaca tagccactgc gttgagcaga agcggataca acattgctgt ggtagataca 28080 gatccacaaa tgagcctgac gaactggagc aaagcgggca aggcagcatt· tgacgtattt 28140 acagctgcat ctgaaaaaga cgtctatgga atccgaaaag atctggcgga ctatgacttt 28200 gctattgtgg acggggcagg ttcgctctca gtaatcacct ccgcagccgt catggtaagc 28260 gatctggtaa ttatccctgt tacacccagc cccctggatt tctccgcagc aggaagcgtc 28320 gttactgttc tggaagcaca ggcttacagc cgcaaagttg aagcccgctt tctgatcacc 28380 cgtaagatag aaatggcaac catgctcaat gtgctgaaag aaagtatcaa agacactggt 28440 gttaaatctt tccgtacggc cattacacaa cgtcaggttt acgtgaaatc aattctggat 28500 ggtgacagcg tgtttgaatc cagtgatggc gcagcaaaag gtgaaataga aatccttaca 28560 aaagagatag ttagcacatt tgagtaatta ctcattcact catataatca aataagtata 28620 acaaccggag taaccttaat gtcacttgaa aaagcgcata cggcagtaaa aaaaatgacc 28680 tttggtgaaa acagagatct ggaacgagta gtaacagcac cagtatcatc tggaaaaatc 28740 aaacgtgtta acgtcaattt tgacgaagaa aaacacaccc ggtttaaggc tgcatgtgcc 28800 aaaaaaggta catcgatcac agatgtggtg aaccagcttg tagataaatg gctcaaagag 28860 aacgaataat atctgaggat atatcatgga taaaaacgtc gttgatgcgc tgaaaacgct 28920 attagaagcg ttaccggaag aggtggtaac agaagtcaca tcaaaactaa atccttcggc 28980 aagccatatt cctgaagaaa acagtaagca attgacagca aaagcaagac tactgaattt 29040 ccggctaacc gaagcctatg aagaaatcct ggaagtcgaa gctatcagaa caggccagag 29100 caaaactacc gttctaaagg cagcactggc gatgtacaac agccaggatg aaaacataaa 29160 gaatcactgg ctacttgagt ctgcaaaaat taactaattc aggatataga ttattagtag 29220 gagttcgggc aataatggat gataactcta cgtcagtaac cagcaaagaa gatgtgcagg 29280 atatttcttt aaaaaactgg ctaaagaata aaaaaaccat tgtattactt atatttactg 29340 cattaacaaa tgttggcaat gtcctttcga cgattgatat tatcacagat aaaaccagtt 29400 ctttttacac atggcttggg gaatcgaaaa aattcgaagg ccattggacc aacaatacag 29460 aaggattcat agacggcact cctgattttt tactcaagaa tgcaggcgat gtcctcataa 29520 aatttgacct gaacatcaaa ggtggcgaag tgaggggaga gcttcacacc gacgcacgac 29580 taaaaatgtg tgaaactata gaagaaaagt taaaaacttt gtgtacatta atagcctcac 29640 atccactaat gattgagggg gaaaaatcac cattttccaa tgaattcgat gcctatatta 29700 ctgagtatag aaatggagat aaaaaaatag tcgcaatatt aaatatgaaa attacagacg 29760 atgacaaaat gacaataaca aatacaatga gaacaccaga atcagcatta ttttcaacta 29820 aaatatatgc tataaaaata ataaccgaag aataacttaa taacaaaaaa ggtattaaag 29880 tgaatactgg aataacaatt gatttgacca acctttcaga agatgaattg cttgatttat 29940 attcaatgta taaaagtgca aacatagcac atcaactatg gtgcagacgc catgaaaata 30000 taccagagca tttttcgata atattcgtga cgcttttaga acgaataaaa agggttacag 30060 aaaagaactc agaaggggta aaaacaccag atgtagacct ggatgcatta attgacacca 30120 tttatattgg ttgtcgttca atgttctgtg aaaaccctgg cttaaaaaat aactacactc 30180 tgcaaaactg cctgaggaaa gccaattatc acaacgaagc tagagtgata gataatattt 30240 tacaggaaaa aaaattcaca gattccatca tgaaagatga gtcatttttt agtctggtga 30300 aattagtttc caataagtcc attgcacacc aggagagcct ttcaggaaaa aaacgggaaa 30360 agatagacta tcgatataaa ttcttaaatg acaattcaaa tatctgtgag tttcagtatt 30420 acatttttag atgtcaccgt atttatgaga atattgtgaa agaatatggg gatacattac 30480 tgaatgagct taaaataaaa aacaatgata tataaagaat gcccccatat gaatagaagg 30540 aagaatcata ctaataaatt ttccagctta tcgctggtaa gcaattctgc cagcgaatca 30600 catttcataa aatcaatatc catttcgtcc agaacatcac agtagctgac atcatcaaga 30660 ctaatgacaa tcacaggaac accctctttg agatcaaaca ctaagacagg taatgaaatg 30720 gagtttatct tttcaaaatc tgttcagttc catattcact ggcaggaaaa 30780 agaatctcac ttaatctcac acaataaaaa acatgatagt gttctttaag gttatccttt 30840 aaaatattaa cagccttagt atatttttcg ttaataaact ctttgaccaa atatttcata 30900 aaactctcca acaagaaccg actgtaggtc atcgggcaaa cgttgcggaa tggcgtcaga 30960 gacgtcattt tgcggcgttt gccctatcct gcatcgcagt ggcatcattt ctggcttatc 31020 tcgctactgt tctcggtgct ttcgtccgca ggttcgggaa caagtaactt gtgccagggc 31080 atagttttgt tgagcagctc catcagaggc tcagccagca gtcccaaaat ccaggttgcc 31140 22ctcaatataa tagctgcaaa tgatcaggcc tctttccctg gcaaaagcct cgatagtttc 27600 cagggcacgc gaagcatcct gttctgctgt tgaagctctc agataagcgc ggacaaagtt 27660 tgttggtgca tttgaagtca tattacagaa ccagttcgca taaggtcaca ccactatacc 27720 caaaatgaac cagaaagtgg atcgacagga aaggcacact ctaaaagaac cgaaaaaaca 27780 aataatgagt aaatgtgaca tcgtcaccca tactcataaa ctcacatgct catatacaca 27840 acaaactcaa atgagtatgt gctctttaac tcataaactc atattaacct ttactcatgc 27900 aaagagtatg gtaaactcaa aaactcacaa gagtaattga gtagatacac aattgagggt 27960 ttgagtatga aagtaatctc atttctgaat ccgaaagggg gttcaggtaa aacaactgcc 28020 gtaatcaaca tagccactgc gttgagcaga agcggataca acattgctgt ggtagataca 28080 gatccacaaa tgagcctgac gaactggagc aaagcgggca aggcagcatt · tgacgtattt 28140 acagctgcat ctgaaaaaga cgtctatgga atccgaaaag atctggcgga ctatgacttt 28200 gctattgtgg acggggcagg ttcgctctca gtaatcacct ccgcagccgt catggtaagc 28260 gatctggtaa ttatccctgt tacacccagc cccctggatt tctccgcagc aggaagcgtc 28320 gttactgttc tggaagcaca ggcttacagc cgcaaagttg aagcccgctt tctgatc acc 28380 cgtaagatag aaatggcaac catgctcaat gtgctgaaag aaagtatcaa agacactggt 28440 gttaaatctt tccgtacggc cattacacaa cgtcaggttt acgtgaaatc aattctggat 28500 ggtgacagcg tgtttgaatc cagtgatggc gcagcaaaag gtgaaataga aatccttaca 28560 aaagagatag ttagcacatt tgagtaatta ctcattcact catataatca aataagtata 28620 acaaccggag taaccttaat gtcacttgaa aaagcgcata cggcagtaaa aaaaatgacc 28680 tttggtgaaa acagagatct ggaacgagta gtaacagcac cagtatcatc tggaaaaatc 28740 aaacgtgtta acgtcaattt tgacgaagaa aaacacaccc ggtttaaggc tgcatgtgcc 28800 aaaaaaggta catcgatcac agatgtggtg aaccagcttg tagataaatg gctcaaagag 28860 aacgaataat atctgaggat atatcatgga taaaaacgtc gttgatgcgc tgaaaacgct 28920 attagaagcg ttaccggaag aggtggtaac agaagtcaca tcaaaactaa atccttcggc 28980 aagccatatt cctgaagaaa acagtaagca attgacagca aaagcaagac tactgaattt 29040 ccggctaacc gaagcctatg aagaaatcct ggaagtcgaa gctatcagaa caggccagag 29100 caaaactacc gttctaaagg cagcactggc gatgtacaac agccaggatg aaaacataaa 29160 gaatcactgg ctacttgagt ctgcaaaaat taactaattc aggatataga ttattagtag 29220 gagttcgggc aataatggat gataactcta cgtcagtaac cagcaaagaa gatgtgcagg 29280 atatttcttt aaaaaactgg ctaaagaata aaaaaaccat tgtattactt atatttactg 29340 cattaacaaa tgttggcaat gtcctttcga cgattgatat tatcacagat aaaaccagtt 29400 ctttttacac atggcttggg gaatcgaaaa aattcgaagg ccattggacc aacaatacag 29460 aaggattcat agacggcact cctgattttt tactcaagaa tgcaggcgat gtcctcataa 29520 aatttgacct gaacatcaaa ggtggcgaag tgaggggaga gcttcacacc gacgcacgac 29580 taaaaatgtg tgaaactata gaagaaaagt taaaaacttt gtgtacatta atagcctcac 29640 atccactaat gattgagggg gaaaaatcac cattttccaa tgaattcgat gcctatatta 29700 ctgagtatag aaatggagat aaaaaaatag tcgcaatatt aaatatgaaa attacagacg 29760 atgacaaaat gacaataaca aatacaatga gaacaccaga atcagcatta ttttcaacta 29820 aaatatatgc tataaaaata ataaccgaag aataacttaa taacaaaaaa ggtattaaag 29880 tgaatactgg aataacaatt gatttgacca acctttcaga agatgaattg cttgatttat 29940 attcaatgta taaaagtgca aacatagcac atcaactatg gtgcagacgc catgaaaata 30000 taccagagca tttttcgata atattcgtga cgcttttaga ac gaataaaa agggttacag 30060 aaaagaactc agaaggggta aaaacaccag atgtagacct ggatgcatta attgacacca 30120 tttatattgg ttgtcgttca atgttctgtg aaaaccctgg cttaaaaaat aactacactc 30180 tgcaaaactg cctgaggaaa gccaattatc acaacgaagc tagagtgata gataatattt 30240 tacaggaaaa aaaattcaca gattccatca tgaaagatga gtcatttttt agtctggtga 30300 aattagtttc caataagtcc attgcacacc aggagagcct ttcaggaaaa aaacgggaaa 30360 agatagacta tcgatataaa ttcttaaatg acaattcaaa tatctgtgag tttcagtatt 30420 acatttttag atgtcaccgt atttatgaga atattgtgaa agaatatggg gatacattac 30480 tgaatgagct taaaataaaa aacaatgata tataaagaat gcccccatat gaatagaagg 30540 aagaatcata ctaataaatt ttccagctta tcgctggtaa gcaattctgc cagcgaatca 30600 catttcataa aatcaatatc catttcgtcc agaacatcac agtagctgac atcatcaaga 30660 ctaatgacaa tcacaggaac accctctttg agatcaaaca ctaagacagg taatgaaatg 30720 gagtttatct tttcaaaatc tgttcagttc catattcact ggcaggaaaa 30780 agaatctcac ttaatctcac aaaatattaa cagccttagt atatttttcg acaataaaaa acatgatagt gttctttaag gttatccttt 30840 ttaataaact ctttg accaa atatttcata 30900 aaactctcca acaagaaccg actgtaggtc atcgggcaaa cgttgcggaa tggcgtcaga 30960 gacgtcattt tgcggcgttt gccctatcct gcatcgcagt ggcatcattt ctggcttatc 31020 tcgctactgt tctcggtgct ttcgtccgca ggttcgggaa caagtaactt gtgccagggc 31080 atagttttgt tgagcagctc catcagaggc tcagccagca gtcccaaaat ccaggttgcc 31140 22

201142028 gtaatcaccg gtatagtgag attttgtggc gtccataact ggtgatcgag atgataccag gtgatgacac acaaaatacc acccagccag aacacagccc atttaaacgc accaagaatg gctaacacaa aaacaaagat aaaatgtaaa acactcgcgg caagaagacg aagataaaac catccccagt caagccactt aacaaaagtg ctccctgttt tcactgcaaa acgtttctta cgctccctgg cgatttcttt cctgaattta gcacgttctt ccccctgagg gaattgatag attttagcca tgagttacct catttcgatc tcaaaacgaa gtttatgcaa ttatcctctg aaaccgtatg gtattctgat atctaggcct tcactttctt tatctatttt ataagaaaac cttagctttg atgctcttct tgcgactctc tcaacgattt taacttcgag atgattaatc tctgtatatt gatttatttt ttcaatgttt ttttgcagaa aaactttatt aaagtcctta aaattctgat aaaggtaaac tttttcgcca ttatctattc tgaataattc aagttcatcc tttaatacat ctacttcaat gtcaaaaaat gtctttttac cactgctgta ttgtttcctg ataaactgat aaagcatatt tgaattttga tcagcaagtc ttacagacga tatcaaaacc tgagtagtgt aagaatcctt taacatcgtg atatatggct caatgacatg tgaaaaagca atatcaatat atccttcact ttcaacataa tcacaaaacg cagtcaggtt cataagcctg actgcatcag aaggtaattt acctctcttc ttcgtatgtc cagagatttg attgccctga gctgtgagaa gttgatttcg aggaatgcga ataacactgg cctgaagttc ttctgccccc tctttcaact gccggtaagc acctgttaca tcaatatcag cgataaatgc atattcccga gccgtaatac gaaaggttgc cccctcagat aactcttcac gtgaatcaat ctgcgccata gccataaaca aaattcttct ggcagacaaa ggcaacgatg agaatgtgct gttaatttca tttctgtggc gaatttttgt tttctttgta acgctcagta agttcataca ttacctgtgg tttttaagtt ctaatggtga aatcataacc acaaaggcgg aaatatgtaa agtggtttat ccaccttatg tatttccacc aaaacacgga tcacatgaaa gaatttttat cattaatatt caatatgata gaaatgcgca ggtatccacc ttttaacgtc atacgttaga ttagagcact ataaagaact aaagaaatga agatacaaag atacgaatat ctgaataaaa acaggaaaat gcgtacccat ccacctttca gtgcgtaccc atccaccttt cagtgcgcag ccatccacct ttcatgaaaa ttacagtgcg tacccatcca cctttcagtg cgtacccatc cacctttcat tttgaggcga caaaaaacaa cacctatctt aatgaaatta ttgataaaaa aactggcaca atgtttgcag aataagttaa agtaagttaa agatcttaaa gtagatctac cgatctacat ttaagatctt aaaaacaaga agtttaataa gcaaaaaaac acaaaaaatc tgtggataac tttgctcaaa acaaaaaatt cacactacct gtggataact ttgcgtaaac ccggaggaca gatcactctg <210> SEQ ID NO 3 <211>837201142028 gtaatcaccg gtatagtgag attttgtggc gtccataact ggtgatcgag atgataccag gtgatgacac acaaaatacc acccagccag aacacagccc atttaaacgc accaagaatg gctaacacaa aaacaaagat aaaatgtaaa acactcgcgg caagaagacg aagataaaac catccccagt caagccactt aacaaaagtg ctccctgttt tcactgcaaa acgtttctta cgctccctgg cgatttcttt cctgaattta gcacgttctt ccccctgagg gaattgatag attttagcca tgagttacct catttcgatc tcaaaacgaa gtttatgcaa ttatcctctg aaaccgtatg gtattctgat atctaggcct tcactttctt tatctatttt ataagaaaac cttagctttg atgctcttct tgcgactctc tcaacgattt taacttcgag atgattaatc tctgtatatt gatttatttt ttcaatgttt ttttgcagaa aaactttatt aaagtcctta aaattctgat aaaggtaaac tttttcgcca ttatctattc tgaataattc aagttcatcc tttaatacat ctacttcaat gtcaaaaaat gtctttttac cactgctgta ttgtttcctg ataaactgat aaagcatatt tgaattttga tcagcaagtc ttacagacga tatcaaaacc tgagtagtgt aagaatcctt taacatcgtg atatatggct caatgacatg tgaaaaagca atatcaatat atccttcact ttcaacataa tcacaaaacg cagtcaggtt cataagcctg actgcatcag aaggtaattt acctctcttc ttcgtatgtc cagagatttg attgccctga gctgtgagaa gttgatttcg aggaatgcga ataacactgg cctgaagttc ttctgccccc tctttcaact gccggtaagc acctgttaca tcaatatcag cgataaatgc atattcccga gccgtaatac gaaaggttgc cccctcagat aactcttcac gtgaatcaat ctgcgccata gccataaaca aaattcttct ggcagacaaa ggcaacgatg agaatgtgct gttaatttca tttctgtggc gaatttttgt tttctttgta acgctcagta agttcataca ttacctgtgg tttttaagtt ctaatggtga aatcataacc acaaaggcgg aaatatgtaa agtggtttat ccaccttatg tatttccacc aaaacacgga tcacatgaaa gaatttttat cattaatatt caatatgata gaaatgcgca ggtatccacc ttttaacgtc atacgttaga ttagagcact ataaagaact aaagaaatga agatacaaag atacgaatat ctgaataaaa acaggaaaat gcgtacccat ccacctttca gtgcgtaccc atccaccttt cagtgcgcag ccatccacct ttcatgaaaa ttacagtgcg tacccatcca cctttcagtg cgtacccatc cacctttcat tttgaggcga caaaaaacaa cacctatctt aatgaaatta ttgataaaaa aactggcaca atgtttgcag aataagttaa agtaagttaa agatcttaaa gtagatctac cgatctacat ttaagatctt aaaaacaaga agtttaataa gcaaaaaaac acaaaaaatc tgtggataac tttgctcaaa acaaaaaatt cacactacct gtggataact ttgcgtaaac ccggaggaca gatcactctg <210> SEQ ID NO 3 <211>837

<212>DNA <213>腸炎沙門氏菌 <400>3 atgaacttac tgagcgttac aaagaaaaca aaaattcgcc acagaaatga aattaacagc acattctcat cgttgccttt gtctgccaga agaattttgt ttatggctat ggcgcagatt gattcacgtg aagagttatc tgagggggca acctttcgta ttacggctcg ggaatatgca tttatcgctg atattgatgt aacaggtgct taccggcagt tgaaagaggg ggcagaagaa cttcaggcca gtgttattcg cattcctcga aatcaacttc tcacagctca gggcaatcaa atctctggac atacgaagaa gagaggtaaa ttaccttctg atgcagtcag gcttatgaac ctgactgcgt tttgtgatta tgttgaaagt gaaggatata ttgatattgc tttttcacat gtcattgagc catatatcac gatgttaaag gattcttaca ctactcaggt tttgatatcg tctgtaagac ttgctgatca aaattcaaat atgctttatc agtttatcag gaaacaatac agcagtggta aaaagacatt ttttgacatt gaagtagatg tattaaagga tgaacttgaa ttattcagaa tagataatgg cgaaaaagtt tacctttatc agaattttaa ggactttaat aaagtttttc tgcaaaaaaa cattgaaaaa ataaatcaat atacagagat taatcatctc gaagttaaaa tcgttgagag agtcgcaaga agagcatcaa agctaaggtt ttcttataaa atagataaag aaagtgaagg cctagatatc agaataccat acggtttcag aggataa <210> SEQ ID NO 4 <211>231 31200 31260 31320 31380 31440 31500 31560 31620 31680 31740 31800 31860 31920 31980 32040 32100 32160 32220 32280 32340 32400 32460 32520 32580 32640 32700 32760 32820 32880 32940 32950 60 120 180 240 300 360 420 480 540 600 660 720 780 837 23 201142028 <212>DNA <213>腸炎沙門氏菌 <400>4 atgtcacttg aaaaagcgca tacggcagta aaaaaaatga cctttggtga aaacagagat ctggaacgag tagtaacagc accagtatca tctggaaaaa tcaaacgtgt taacgtcaat tttgacgaag aaaaacacac ccggtttaag gctgcatgtg ccaaaaaagg tacatcgatc acagatgtgg tgaaccagct tgtagataaa tggctcaaag agaacgaata a <210> SEQ ID NO 5 <211>621gagaggtaaa ttaccttctg 3 atgaacttac tgagcgttac aaagaaaaca aaaattcgcc acagaaatga aattaacagc acattctcat cgttgccttt gtctgccaga agaattttgt ttatggctat ggcgcagatt gattcacgtg aagagttatc tgagggggca acctttcgta ttacggctcg ggaatatgca tttatcgctg atattgatgt aacaggtgct taccggcagt tgaaagaggg ggcagaagaa cttcaggcca gtgttattcg cattcctcga aatcaacttc tcacagctca gggcaatcaa atctctggac atacgaagaa; < 212 > DNA < 213 > Salmonella enteritidis < 400 & gt atgcagtcag gcttatgaac ctgactgcgt tttgtgatta tgttgaaagt gaaggatata ttgatattgc tttttcacat gtcattgagc catatatcac gatgttaaag gattcttaca ctactcaggt tttgatatcg tctgtaagac ttgctgatca aaattcaaat atgctttatc agtttatcag gaaacaatac agcagtggta aaaagacatt ttttgacatt gaagtagatg tattaaagga tgaacttgaa ttattcagaa tagataatgg cgaaaaagtt tacctttatc agaattttaa ggactttaat aaagtttttc tgcaaaaaaa cattgaaaaa ataaatcaat atacagagat taatcatctc gaagttaaaa tcgttgagag agtcgcaaga agagcatcaa agctaaggtt ttcttataaa atagataaag aaagtgaagg cctagatatc agaataccat acggtttcag aggataa <210> SEQ ID NO 4 <211>231 31200 31260 31320 31380 31440 31500 31560 31620 31680 31740 31800 31860 31920 31980 32040 32100 32160 32220 32280 32340 32400 32460 32520 32580 32640 32700 32760 32820 32880 32940 32950 60 120 180 240 300 360 420 480 540 600 660 720 780 837 23 201142028 < 212 > DNA < 213 > Salmonella enteritidis < 400 > 4 atgtcacttg aaaaagcgca tacggcagta aaaaaaatga cctttggtga aaacagagat ctggaacgag tagtaacagc accagtatca tctggaaaaa tcaaacgtgt taacgtcaat tttgacgaag aaaaacacac ccggtttaag gctgcatgtg ccaaaaaagg tacatcgatc acagatgtgg tgaaccagct tgtagataaa tggctcaaag agaacgaata a < 210 > SEQ ID NO 5 <211>621

<212>DNA <213〉腸炎沙門氏菌 <400>5 atgaaagtaa tctcatttct gaatccgaaa gggggttcag gtaaaacaac tgccgtaatc aacatagcca ctgcgttgag cagaagcgga tacaacattg ctgtggtaga tacagatcca caaatgagcc tgacgaactg gagcaaagcg ggcaaggcag catttgacgt atttacagct gcatctgaaa aagacgtcta tggaatccga aaagatctgg cggactatga ctttgctatt gtggacgggg caggttcgct ctcagtaatc acctccgcag ccgtcatggt aagcgatctg gtaattatcc ctgttacacc cagccccctg gatttctccg cagcaggaag cgtcgttact gttctggaag cacaggctta cagccgcaaa gttgaagccc gctttctgat cacccgtaag atagaaatgg caaccatgct caatgtgctg aaagaaagta tcaaagacac tggtgttaaa tctttccgta cggccattac acaacgtcag gtttacgtga aatcaattct ggatggtgac agcgtgtttg aatccagtga tggcgcagca aaaggtgaaa tagaaatcct tacaaaagag atagttagca catttgagta a <210> SEQ 工D NO 6 <211>252&Lt; 212 > DNA < 213> Salmonella enteritidis < 400 > 5 atgaaagtaa tctcatttct gaatccgaaa gggggttcag gtaaaacaac tgccgtaatc aacatagcca ctgcgttgag cagaagcgga tacaacattg ctgtggtaga tacagatcca caaatgagcc tgacgaactg gagcaaagcg ggcaaggcag catttgacgt atttacagct gcatctgaaa aagacgtcta tggaatccga aaagatctgg cggactatga ctttgctatt gtggacgggg caggttcgct ctcagtaatc acctccgcag ccgtcatggt aagcgatctg gtaattatcc ctgttacacc cagccccctg gatttctccg cagcaggaag cgtcgttact gttctggaag cacaggctta cagccgcaaa gttgaagccc gctttctgat cacccgtaag atagaaatgg caaccatgct caatgtgctg aaagaaagta tcaaagacac tggtgttaaa tctttccgta cggccattac acaacgtcag gtttacgtga aatcaattct ggatggtgac agcgtgtttg aatccagtga tggcgcagca aaaggtgaaa tagaaatcct tacaaaagag atagttagca catttgagta a < 210 > SEQ station D NO 6 < 211 > 252

<212>DNA <213>腸炎沙門氏菌 <400>6 atgagctatc aaattctgac aaccacagcg gccagtatta ctgacctgaa aaaaaatcct atgggaaccg tagctgaagg tgaaggggac gctgttgcga tcctgaaccg aaacgaaccg gcgttctatt gcgttccacc aaaactttac gcctactatc gggaactcgc tgaagatgct gagttaaacg ctgttgctga tgagcgcatg aaaaacccgg aaattgtgaa ggttaacctg gatgacctat ga <210> SEQ ID NO 7 <211>282 <212>DNA <213>腸炎沙門氏菌 <400>7 atgacctatg aactggcttt tgaccgtaga gcactgaagg aatggcagaa actcggccac accatccgtg aacaattcaa aaagaaactg gcagaacggc tggaaaatcc acgcgtaccc gcagcccggt tacatggtca tgctgatcgc tataaaatca aacttcgtgc atctggctac agacttgtat atcaagtcat tgatgagaaa gtcgttttac ttgttatttc cgttggaaga agggaaagca gcgaagtcta tcagatcgca gatttgcgct aa <210> SEQ ID NO 8 60 120 180 231 60 120 180 240 300 360 420 480 540 600 621 60 120 180 240 252 60 120 180 240 282 24 201142028 <211>648 <212>DNA <213>腸炎沙門氏菌 <400>8 atgttatcta gacacaacgc ataataccaa tcaaaggagg gtaggactta atgtttgatc cgaggtggcg gtaaagccag cctgataata aagacgacta gtatcaaatg cttctacttt acgaagtcgc aggttaaacg cagcacttaa tgcaaataac catgcgaaaa acttagtgcg aacctgaatt aaaaaagttt tcacacctga aaaaggaaac tcttgcgctt cagggttgaa taaaactggt gatcgttaaa gagtaccaat tcttaaggta tgggctgagt taataataca tattgttccc agaaatcatt aaaagatgtt gccatgcaat tcaggtttta gataaaggcg aacattgaat tttgcaaagt tcagaaaaaa tgctattatt agttatgtac tctgtaaagg atatatcctc gaaattaaat gcgctgccag acccatatgc tagtatctta tacggaatca tcggtacaac tactggttga ctggcaatca aacgtatagg aaatgattaa aatacgccat ctgaataa cgttcatccc gattgcggaa ctttcctgaa tcgttactct agcagagaaa ctgttataaa agaaacagga atttagccct aaaggagaat gcgtggcact 00000000008 62840628404 1123344566 <210> SEQ ID NO 9 <211>297&Lt; 212 > DNA < 213 > Salmonella enteritidis < 400 > 6 atgagctatc aaattctgac aaccacagcg gccagtatta ctgacctgaa aaaaaatcct atgggaaccg tagctgaagg tgaaggggac gctgttgcga tcctgaaccg aaacgaaccg gcgttctatt gcgttccacc aaaactttac gcctactatc gggaactcgc tgaagatgct gagttaaacg ctgttgctga tgagcgcatg aaaaacccgg aaattgtgaa ggttaacctg gatgacctat ga < 210 > SEQ ID NO 7 < 211 > 282 < 212 > DNA < 213 > Salmonella enteritidis < 400 > 7 atgacctatg aactggcttt tgaccgtaga gcactgaagg aatggcagaa actcggccac accatccgtg aacaattcaa aaagaaactg gcagaacggc tggaaaatcc acgcgtaccc gcagcccggt tacatggtca tgctgatcgc tataaaatca aacttcgtgc atctggctac agacttgtat atcaagtcat tgatgagaaa gtcgttttac ttgttatttc cgttggaaga agggaaagca gcgaagtcta tcagatcgca gatttgcgct aa <210> SEQ ID NO 8 60 120 180 231 60 120 180 240 300 360 420 480 540 600 621 60 120 180 240 252 60 120 180 240 282 24 201142028 <211>648 <212>DNA <213> Salmonella <400>8 atgttatcta gacacaacgc ataataccaa tcaaaggagg gtagga ctta atgtttgatc cgaggtggcg gtaaagccag cctgataata aagacgacta gtatcaaatg cttctacttt acgaagtcgc aggttaaacg cagcacttaa tgcaaataac catgcgaaaa acttagtgcg aacctgaatt aaaaaagttt tcacacctga aaaaggaaac tcttgcgctt cagggttgaa taaaactggt gatcgttaaa gagtaccaat tcttaaggta tgggctgagt taataataca tattgttccc agaaatcatt aaaagatgtt gccatgcaat tcaggtttta gataaaggcg aacattgaat tttgcaaagt tcagaaaaaa tgctattatt agttatgtac tctgtaaagg atatatcctc gaaattaaat gcgctgccag acccatatgc tagtatctta tacggaatca tcggtacaac tactggttga ctggcaatca aacgtatagg aaatgattaa aatacgccat Ctgaataa cgttcatccc gattgcggaa ctttcctgaa tcgttactct agcagagaaa ctgttataaa agaaacagga atttagccct aaaggagaat gcgtggcact 00000000008 62840628404 1123344566 <210> SEQ ID NO 9 <211>297

<212>DNA <213>腸炎沙門氏菌 <400>9 atgttgaaat atgctttgtg ttacagaaaa cttgttattg gttatcggaa taaatctgaa ttgcagaacc taattgattt ggtatattgc ttatcatctt taaacgttat tgcttttgcg tttaacggat gatattttct tattgttgca ttaacgcttt gatgatgtgt attcgtaaac cgtcaacata ccatatattc ctctatttat ctacgaagac ctgcaattac cctcatggat ctgactggct ggctgcgttg aactggtttt aattattgct agtcccactt tgcgtaa 2 2 6020804097 <210> SEQ ID N〇 10 <211>2754&Lt; 212 > DNA < 213 > Salmonella enteritidis < 400 > 9 atgttgaaat atgctttgtg ttacagaaaa cttgttattg gttatcggaa taaatctgaa ttgcagaacc taattgattt ggtatattgc ttatcatctt taaacgttat tgcttttgcg tttaacggat gatattttct tattgttgca ttaacgcttt gatgatgtgt attcgtaaac cgtcaacata ccatatattc ctctatttat ctacgaagac ctgcaattac cctcatggat ctgactggct ggctgcgttg aactggtttt aattattgct agtcccactt tgcgtaa 2 2 6020804097 <210> SEQ ID N〇10 <211>2754

<212>DNA <213>腸炎沙門氏菌 tttttaaagg tcttacgcgc ttcttggaat gtgcattatt cccttattct tcctggctgg cttactttga gtgtgttgat ttatacgtag ctttgaaggg agggcgtcag accagtattt attacgagtc attccctcag ttaaaccttt tactacggaa aagatatctt taaagagcct atctgtcccg ttttcattcc cagatcagtt atctctgttc gcagtagtcc gttttataca <400>10 atgagtactc ctctacccct gctatgtatg gaacgctttt aagcgattca gtggataact tcacatatca atagacggtg cttaatacgc aggtgtaaaa atgaatgatt acgatttgtt aaaaaacaaa ttgaatacct gttgtttatt gtcagggtta aatgatgccg aaggattatt gagtatgtcg gatgatcaga ttgaccttct gcgcagtcca ttatgaagct ctgataatat gacaaattac ttcaggagac tgacatcgtc tcgataagct gtatctgaga ttatgcgggg gaaagaccag tatcgttaca cgcgtcagac ggatgccggt ctttactgcg ggaaatgaca cctgctttaa tgtgttctgc tatgccatca actcttgtca agccagtaca tcttctgttg aaaaaccggg tctgaagatt aaatttgtta aacagtaaaa tctgaatttt gataaagtaa gttaatgaaa cgacgtcttg cagtatctgc tatctgggag catgcccggt attctggatg atggataagc gatgatgctg taagggggct ttggtgtctg ggcgtgtaac aagggcatcc atgaagttga aagagttaat atttgctggc tgtcaattct cgctttatcc ttccttttgt tcgagaataa cacatttctt tgaatgaaat ggctttatga tttctggtcg gaaaagacct attttcgttg ctctgatgta agtcagcgat ccaaatcttt gggcgttccg gattcatgag acagtttgat tttatcaaac tatttcaaaa tccgtactct aacctggcag gacagatcag tcatcgtatc gaacagagta attatttctg ttcacgcagt ttgcgacagg agatcatggg atggcaaaag gttctttggt catagagatt tctccccgtt taaggcgctg gctggcagat&Lt; 212 > DNA < 213 > Salmonella enteritidis tttttaaagg tcttacgcgc ttcttggaat gtgcattatt cccttattct tcctggctgg cttactttga gtgtgttgat ttatacgtag ctttgaaggg agggcgtcag accagtattt attacgagtc attccctcag ttaaaccttt tactacggaa aagatatctt taaagagcct atctgtcccg ttttcattcc cagatcagtt atctctgttc gcagtagtcc gttttataca < 400 > 10 atgagtactc ctctacccct gctatgtatg gaacgctttt aagcgattca gtggataact tcacatatca atagacggtg cttaatacgc aggtgtaaaa atgaatgatt acgatttgtt aaaaaacaaa ttgaatacct gttgtttatt gtcagggtta aatgatgccg aaggattatt gagtatgtcg gatgatcaga ttgaccttct gcgcagtcca ttatgaagct ctgataatat gacaaattac ttcaggagac tgacatcgtc tcgataagct gtatctgaga ttatgcgggg gaaagaccag tatcgttaca cgcgtcagac ggatgccggt ctttactgcg ggaaatgaca cctgctttaa tgtgttctgc tatgccatca actcttgtca agccagtaca tcttctgttg aaaaaccggg tctgaagatt aaatttgtta aacagtaaaa tctgaatttt gataaagtaa gttaatgaaa cgacgtcttg cagtatctgc tatctgggag catgcccggt attctggatg atggataagc gatgatgctg taagggggct ttggtgtctg ggcgtgtaac aagggcatcc Atgaagttga aagagt taat atttgctggc tgtcaattct cgctttatcc ttccttttgt tcgagaataa cacatttctt tgaatgaaat ggctttatga tttctggtcg gaaaagacct attttcgttg ctctgatgta agtcagcgat ccaaatcttt gggcgttccg gattcatgag acagtttgat tttatcaaac tatttcaaaa tccgtactct aacctggcag gacagatcag tcatcgtatc gaacagagta attatttctg ttcacgcagt ttgcgacagg agatcatggg atggcaaaag gttctttggt catagagatt tctccccgtt taaggcgctg gctggcagat

oooooooooooooooooooo 62840628406284062840 1123344566778990012 lx ΙΑ IX 25 201142028Oooooooooooooooooooo 62840628406284062840 1123344566778990012 lx ΙΑ IX 25 201142028

ctgaaagtcg gactggatat ggtttccagt ggatatattt ctttcggaaa atcgcatcag 1260 accctggttg tctttgcgga ttcaccggag cggctggtga aagacaccaa tatcgtgact 1320 tccactctgg aagatttggg gctgattgtc acttattcaa cactgagtct tggcgcagct 1380 tattttgctc agctaccagg aaattatacg cttcgccctc gtctgagtac cctcagtagt 1440 cttaattttg ccgaaatgga aagttttcat aatttctttt caggaaaaga aaaaggaaat 1500 acctgggggg aaaaactgat tactcttcgg gggtcaggta atgatatcta ccatctgaat 1560 taccatatga cgactgaaca tcagaatttc ttcggtaaga acccgacgct ggggcatacc 1620 gaaattctcg gtacgtctaa cgtgggtaaa accgtattac tgatgacaaa agcatttgcc 1680 gcccagcagt tcggtacgcc ggaatcattc cctgcaaaca gaaaactgaa aaaactgacc 1740 acggtttttt ttgataaaga ccgggcaggt gaagtcggta tacgggcaat ggggggatct 1800 tattaccggg tgaaggaggg agagccgaca ggctggaatc ccgccgcact gccgccaaca 1860 aagcgtaata tcgcttttat gaaggacctg gtgaggctgc tttgtactct caacagtgag 1920 ccgctcgatg attaccagaa cagcctgatt tcagatgcgg ttgaacgtct tatgcaacgg 1980 tcagatcgct cttatcctgt cagtaaacta cggcctctta tccaggagcc ggatgatact 2040 gaaaccaaac gtcatggact taaagcccgt cttaagccgt ggacgcaggg ggaagagttt 2100 ggctgggtgt tcgacaatcg ggaagacacg tttgatgtcg ataacctgga tgttttcggt 2160 attgatggaa cggagttcct ggataataag gtgctggcca gtgctgcttc attctatctc 2220 atctatcggg tcaccatgct ggccgatggt cgcaggcttc ttatctacat ggatgagttc 2280 tggcaatgga tcaataacga agcgttcagg gactttgttt acaacaagct gaaaaccgga 2340 cgtaaactcg atatggtgct tgtcgtagcc acacagtcac cggatgaact gattaaatca 2400 cccattgcgg cagcggttcg tgagcaatgc gccactcata tctatctggc aaacccgaaa 2460 gccaaacgta gtgaatatgt tgatggtttg caggtcaggg agctttattt tgacaaaatt 2520 aaagctatcg atccgctgtc ccgccagttc cttgttgtta agaacccaca gaggaaaggt 2580 gaaagtgatg attttgctgc ttttgccaga ctggagctgg gaaaagcagc gtattactta 2640 ccggttctca gtgcatcaaa accccagtta gaactgttcg atgaaatctg gaaagaagga 2700 atgaagccgg aagagtggct tgatacctat ctggaacagg cgaacctgat ttga 2754 <210> SEQ ID NO 11 <211>759ctgaaagtcg gactggatat ggtttccagt ggatatattt ctttcggaaa atcgcatcag 1260 accctggttg tctttgcgga ttcaccggag cggctggtga aagacaccaa tatcgtgact 1320 tccactctgg aagatttggg gctgattgtc acttattcaa cactgagtct tggcgcagct 1380 tattttgctc agctaccagg aaattatacg cttcgccctc gtctgagtac cctcagtagt 1440 cttaattttg ccgaaatgga aagttttcat aatttctttt caggaaaaga aaaaggaaat 1500 acctgggggg aaaaactgat tactcttcgg gggtcaggta atgatatcta ccatctgaat 1560 taccatatga cgactgaaca tcagaatttc ttcggtaaga acccgacgct ggggcatacc 1620 gaaattctcg gtacgtctaa cgtgggtaaa accgtattac tgatgacaaa agcatttgcc 1680 gcccagcagt tcggtacgcc ggaatcattc cctgcaaaca gaaaactgaa aaaactgacc 1740 acggtttttt ttgataaaga ccgggcaggt gaagtcggta tacgggcaat ggggggatct 1800 tattaccggg tgaaggaggg agagccgaca ggctggaatc ccgccgcact gccgccaaca 1860 aagcgtaata tcgcttttat gaaggacctg gtgaggctgc tttgtactct caacagtgag 1920 ccgctcgatg attaccagaa cagcctgatt tcagatgcgg ttgaacgtct tatgcaacgg 1980 tcagatcgct cttatcctgt cagtaaacta cggcctctta tccaggagcc ggatgatact 2040 gaaacc aaac gtcatggact taaagcccgt cttaagccgt ggacgcaggg ggaagagttt 2100 ggctgggtgt tcgacaatcg ggaagacacg tttgatgtcg ataacctgga tgttttcggt 2160 attgatggaa cggagttcct ggataataag gtgctggcca gtgctgcttc attctatctc 2220 atctatcggg tcaccatgct ggccgatggt cgcaggcttc ttatctacat ggatgagttc 2280 tggcaatgga tcaataacga agcgttcagg gactttgttt acaacaagct gaaaaccgga 2340 cgtaaactcg atatggtgct tgtcgtagcc acacagtcac cggatgaact gattaaatca 2400 cccattgcgg cagcggttcg tgagcaatgc gccactcata tctatctggc aaacccgaaa 2460 gccaaacgta gtgaatatgt tgatggtttg caggtcaggg agctttattt tgacaaaatt 2520 aaagctatcg atccgctgtc ccgccagttc cttgttgtta agaacccaca gaggaaaggt 2580 gaaagtgatg attttgctgc ttttgccaga ctggagctgg gaaaagcagc gtattactta 2640 ccggttctca gtgcatcaaa accccagtta gaactgttcg atgaaatctg gaaagaagga 2700 atgaagccgg aagagtggct tgatacctat ctggaacagg cgaacctgat ttga 2754 < 210 > SEQ ID NO 11 < 211 > 759

<212>DNA <213>腸炎沙門氏菌 <400>11 atgaaaaagc aaattatggc ggcattcgtc gcttcactga ttgttatttc cggcgctcag 60 gcagggatac ctgttgccat cgacgccaac cctgaatggg cgattgaagc cggacgatgg 120 acagaacgcc ttaagcaatg ggcggaaacg gtaaaacatt acgaaaatca gataaatgcg 180<212>DNA <213> S. Enteritidis <400>11 atgaaaaagc aaattatggc ggcattcgtc gcttcactga ttgttatttc cggcgctcag 60 gcagggatac ctgttgccat cgacgccaac cctgaatggg cgattgaagc cggacgatgg 120 acagaacgcc ttaagcaatg ggcggaaacg gtaaaacatt acgaaaatca gataaatgcg 180

tacaaacagg agctgctgtc aaaaacgggt atccgtgatg tgcagggact ggtgcagtcc 240 gcacagtcag tgagtcagga actgatgcag atttatgatc aggggaatgc ttttattgac 300 gattacatta aaaatcctga aggggcgtta tcggaacagg ccaaatcgtt attgtcagat 360 tacaaagtaa cggatacctg ccagaacctg ggatattccg gcgacctggt acggggatgt 420 gaagcgacgt tcctttctca actggcaagc gtggaatacg gtaacaagct ggagagcaag 480 cttcgtcagg acaaccagac gatgaaagac cttattgatc aagtcaaaaa tgcgaaggat 540 acgaaggcca cgcaggatgc aacaaacgct .gttgcacttg aacaactgaa gttcgagaag 600 ctcaaatttc agtatcaaat gtatcgcgat aagcagcgag atcttgcaga atataaagag 660 aagatggctc aggcagcttt cagaaaacag caacgtgaag ccgtgccacc ttcttacaga 720 aaagcttata tggcaatgaa atcatatgag gatgattaa 759 <210> SEQ ID NO 12 <211>1134tacaaacagg agctgctgtc aaaaacgggt atccgtgatg tgcagggact ggtgcagtcc 240 gcacagtcag tgagtcagga actgatgcag atttatgatc aggggaatgc ttttattgac 300 gattacatta aaaatcctga aggggcgtta tcggaacagg ccaaatcgtt attgtcagat 360 tacaaagtaa cggatacctg ccagaacctg ggatattccg gcgacctggt acggggatgt 420 gaagcgacgt tcctttctca actggcaagc gtggaatacg gtaacaagct ggagagcaag 480 cttcgtcagg acaaccagac gatgaaagac cttattgatc aagtcaaaaa tgcgaaggat 540 acgaaggcca cgcaggatgc aacaaacgct .gttgcacttg aacaactgaa gttcgagaag 600 Ctcaaatttc agtatcaaat gtatcgcgat aagcagcgag atcttgcaga atataaagag 660 aagatggctc aggcagcttt cagaaaacag caacgtgaag ccgtgccacc ttcttacaga 720 aaagcttata tggcaatgaa atcatatgag gatgattaa 759 <210> SEQ ID NO 12 <211>1134

<212>DNA <213>腸炎沙門氏菌 <400>12 atggggattg tcactggtat accggatggt actgatttat caggtaatgt aacctctccc 60 gcaaatgcgg gaggtgtttc tggatttgat tctgattttt ttcagacaac tcatgaagtt 120 atatttaata ttctcaacaa gagtatatct ggaaaattaa gtgaatattc agatgtggct 180 26 201142028 tatactcttg gtaaatatgg agtttctctg tatgttttat ggtatgcttt tactgtatta gcaaggaaac aacagacacc tgtacctgat tttatctgga atatctgtag gttttacata atattgcttt ttgttaagaa tacaggggga atacttacat cagcaacaga tgctattgat ggattgaaaa atacattggc agggggagat ccgtgggtat ggatggatca gttatgggtg aaggttatac aagttgcaac tcttattttt gataaagata catctactgt gcctgttgcc ggtgggattg gtgctttatt aacttatgtc ggtggtgttt tggcattatt gctttgttct atagtatttg catctgctga attaacatta ctattacttt ctgtcactgc gccaatattt atcatgtgtc tgatgtttgg tttacttcgg caaatgttta atagctggct acagcttaat tttagctcgt tactggtttt tctatttgca gcattagcac taagagctgg gacatggcaa ttaaacatgg cattaagtac gtctattgct acagcatcag aaaacaatct tcttcaaacg ggagtaactt cattagctgc tggcattttc atggcctgga ttatctggca ggcgaaaagt tatgcttcac agattgcagg tgtgggtgtt gaaggtgcca tgcagggcgc agccgctatg gggattggtg ctggcgtttt cggtgcatcc cgtatggcgc gtggcgcact tggtatgggc agaaatgccg gtattggtgc atggaaaggt ctgcgtcgtc aggaagacgg gtttggtcag tctccgggaa taacgggtaa gaccgctaac ctggccggac agggcgttaa tattggtgcc aaaaagcttc gtcaggcagc tattgagaga gcaaagaaaa tgtatggtgg gtaa <210> SEQ 工D NO 13&Lt; 212 > DNA < 213 > Salmonella enteritidis < 400 > 12 atggggattg tcactggtat accggatggt actgatttat caggtaatgt aacctctccc 60 gcaaatgcgg gaggtgtttc tggatttgat tctgattttt ttcagacaac tcatgaagtt 120 atatttaata ttctcaacaa gagtatatct ggaaaattaa gtgaatattc agatgtggct 180 26 201142028 tatactcttg gtaaatatgg agtttctctg tatgttttat ggtatgcttt tactgtatta gcaaggaaac aacagacacc tgtacctgat tttatctgga atatctgtag gttttacata atattgcttt ttgttaagaa tacaggggga atacttacat cagcaacaga tgctattgat ggattgaaaa atacattggc agggggagat ccgtgggtat ggatggatca gttatgggtg aaggttatac aagttgcaac tcttattttt gataaagata catctactgt gcctgttgcc ggtgggattg gtgctttatt aacttatgtc ggtggtgttt tggcattatt gctttgttct atagtatttg catctgctga attaacatta ctattacttt ctgtcactgc gccaatattt atcatgtgtc tgatgtttgg tttacttcgg caaatgttta atagctggct acagcttaat tttagctcgt tactggtttt tctatttgca gcattagcac taagagctgg gacatggcaa ttaaacatgg cattaagtac gtctattgct acagcatcag aaaacaatct tcttcaaacg ggagtaactt Cattagctgc tggcattttc atggcctgga ttatctggca ggcg aaaagt tatgcttcac agattgcagg tgtgggtgtt gaaggtgcca tgcagggcgc agccgctatg gggattggtg ctggcgtttt cggtgcatcc cgtatggcgc gtggcgcact tggtatgggc agaaatgccg gtattggtgc atggaaaggt ctgcgtcgtc aggaagacgg gtttggtcag tctccgggaa taacgggtaa gaccgctaac ctggccggac agggcgttaa tattggtgcc aaaaagcttc gtcaggcagc tattgagaga gcaaagaaaa tgtatggtgg gtaa < 210 > SEQ D NO 13 Workers

<211>129<211>129

<212>DNA <213>腸炎沙門氏菌 <400>13 atgaaactac ttattgtggc tttcgtgacg ctttgtctcg ctggttgtca ggcgtcacat aaactaccgc ccgtttccgg gaaaagcgaa cctgttaatt ctgctgaggt aatgcaaaat ggaatttaa <210> SEQ ID NO 14 <211>714<212>DNA <213> S. Enteritidis <400>13 atgaaactac ttattgtggc tttcgtgacg ctttgtctcg ctggttgtca ggcgtcacat aaactaccgc ccgtttccgg gaaaagcgaa cctgttaatt ctgctgaggt aatgcaaaat ggaatttaa <210> SEQ ID NO 14 <211>

<212>DNA <213>腸炎沙門氏菌 <400>14 atggaattta aacttcccgg atttaaaaat aaaaaagacg ttactgactc atcagtttca tttgaagaaa aaaacattgc actacaggag agaatgaatc gtatttataa attcggtggt atcggaggca tgttaattgg tgggctgtct ttacttgcat taaatgcagc attaccactg aaaacaacag ttgttgatgc ctaccttatc gataaggtta caggtgtggc tgaacgtctg acttctgtta aaaaagaaaa tctttctgaa aacgaagcca ttgcccgata ttttatcacc cagtatataa aacatcgtga aggttataat tttttcagtc tccagcatga ttatgattat gtaatggctt acagcgcgga gaatgtcgcg gcagattata acgcattatt taacagtgaa caggcaccaa aacttgttta taacaaagca gaaaaaacgg caatggttca ggataatcca tctgtcataa tttcaccttc gtcacgggca gatgataaag atatcggtgc gtatattcgt tttcgtctga ccatcaggga tgttgctacc ggacaaaccc gccaggagtt ctggaatgtt cgcctgactt atcgtatcga accgcaggtt gaaatggtgt caggggaacg taataacaat cctcttaaat tcgttgtaac aagctacgtt cgcgataaag aagccagagg ttaa <210> SEQ ID NO 15 <211>930&Lt; 212 > DNA < 213 > Salmonella enteritidis < 400 > 14 atggaattta aacttcccgg atttaaaaat aaaaaagacg ttactgactc atcagtttca tttgaagaaa aaaacattgc actacaggag agaatgaatc gtatttataa attcggtggt atcggaggca tgttaattgg tgggctgtct ttacttgcat taaatgcagc attaccactg aaaacaacag ttgttgatgc ctaccttatc gataaggtta caggtgtggc tgaacgtctg acttctgtta aaaaagaaaa tctttctgaa aacgaagcca ttgcccgata ttttatcacc cagtatataa aacatcgtga aggttataat tttttcagtc tccagcatga ttatgattat gtaatggctt acagcgcgga gaatgtcgcg gcagattata acgcattatt taacagtgaa caggcaccaa aacttgttta taacaaagca gaaaaaacgg caatggttca ggataatcca tctgtcataa tttcaccttc gtcacgggca gatgataaag atatcggtgc gtatattcgt tttcgtctga ccatcaggga tgttgctacc ggacaaaccc gccaggagtt ctggaatgtt cgcctgactt atcgtatcga accgcaggtt gaaatggtgt caggggaacg taataacaat cctcttaaat tcgttgtaac aagctacgtt cgcgataaag aagccagagg ttaa < 210 > SEQ ID NO 15 < 211 > 930

<212>DNA <213>腸炎沙門氏菌 <400>15 atgaaaatga ataaaggagc gttaattatg gcgcttctga tggcggcgca cgtctgtcat gcagctgttc ttccttcagg cagtcgcttt gacccacgca atcagatagt cagttataac 27 201142028 cccaataata gaagatgaaa gaagataacc atggatgaaa gagcttgaac gagctgaacg tatccgcagg gccagacgcc aactggcagt tatgacgatg tttgtgatga tttacggctg gtggggattg ccggatgtta ccaccataat cagttatcag tggtatacct acggtaatac gctggcgaac cccggacgtt aacgccggaa aggaggagca actggaagcg gccgttatac acggtatgca ttggcgtacc agaaccaggg agaaagaggt taacagtgcc tgccagaact ggaagttcgt ctcttctgaa gaatttgttt ccggcagccg ggaacaggcc ggcaatcaac ggttgctgaa ctggttcggc ggagactctt ggttgataag cttcggaaaa gatccagtga gttggataca ggttttccgc cctgttaaac tccgtcagtg gttcgcacca gagaaaattg gaaattgaga cgttccctgg ggcagccagg ttcagtccgt accaatcctg cgttttgttt gtacgtttac ccaccacact agggatgggc agactgttca ttgctcttga cgaagcgtaa cgtttgtggt agaaacgcac aaaatgcgaa atatcagccc taaagaaaat tgattaaaca tacgtcttgg cagccggaga ggtatttgat ggttaataaa gaaaaataat cccggaaaat ttacagcatg gaattaccag agaggctctt atcgccccgt tgattatgca tcccagcgtc gagcgggagt tgagcaggtg tacggtatcc oooooooooooooo 84062840628403 12334456677899 <210> SEQ ID NO 16 <211>1206&Lt; 212 > DNA < 213 > Salmonella enteritidis < 400 > 15 atgaaaatga ataaaggagc gttaattatg gcgcttctga tggcggcgca cgtctgtcat gcagctgttc ttccttcagg cagtcgcttt gacccacgca atcagatagt cagttataac 27 201142028 cccaataata gaagatgaaa gaagataacc atggatgaaa gagcttgaac gagctgaacg tatccgcagg gccagacgcc aactggcagt tatgacgatg tttgtgatga tttacggctg gtggggattg ccggatgtta ccaccataat cagttatcag tggtatacct acggtaatac gctggcgaac cccggacgtt aacgccggaa aggaggagca actggaagcg gccgttatac acggtatgca ttggcgtacc agaaccaggg agaaagaggt taacagtgcc tgccagaact ggaagttcgt ctcttctgaa gaatttgttt ccggcagccg ggaacaggcc ggcaatcaac ggttgctgaa ctggttcggc ggagactctt ggttgataag cttcggaaaa gatccagtga gttggataca ggttttccgc cctgttaaac tccgtcagtg gttcgcacca gagaaaattg gaaattgaga cgttccctgg ggcagccagg ttcagtccgt accaatcctg cgttttgttt gtacgtttac ccaccacact agggatgggc agactgttca ttgctcttga cgaagcgtaa cgtttgtggt agaaacgcac aaaatgcgaa atatcagccc taaagaaaat tgattaaaca tacgtcttgg cagccggaga ggtatttgat ggttaataaa Gaaaaataat cccggaaaat ttac Agcatg gaattaccag agaggctctt atcgccccgt tgattatgca tcccagcgtc gagcgggagt tgagcaggtg tacggtatcc oooooooooooooo 84062840628403 12334456677899 <210> SEQ ID NO 16 <211>

<212>DNA <213>腸炎沙門氏菌 <400>16 gtgactgaac cgcagacaga gcattgcagc gtgggttttc tctgtgtcgt cgtcagaccg aaaccagagg cagagcttca agttcagcgg gatgaagcaa cttccatatg aggcgttttg agcgccagcg gccgggtctt cgcagtaagc gaagccggtg atggtcggga gacagccaga gcattttcta actctgattg ggttaa aggaaaataa aagaactgga ttggtattga ttctgcttgc caggggatga gactgagcga aagaaaaagt gccgtgcact gaacgtcagt aagcaaccgc acccaaattt tttctgacct gtaatgtaaa taaatcatgg cttttattga cttctgggtg tgataccgga ccgactatac atagtgtgaa tcggtcagga aatcccgact acaggccggg aaaacttaaa tgccggtgtt gaaacccgca agatatcgat catttcttct tgacgtttct ttcacatact acagactaca gtttatcccg ggcggggaaa acttatcgaa acaagggcgt tattcccctg gattgacact tttaagtcag ggcaaacagt tattccgcca tctggatttt gcaaccgaaa aagactccgg aagtcacgta tctgtttatt agtcagccgc ccttttaata gaaaagactg cttgatggca gcggccagtg gaatctgcgc gaagggacat ctggaatgta agaggaaccg gtgtttgtta gttgactcac catttcagtg gccgccagtg cgccaggctt acgctttata tcaggcattt ttgaacagca aagaagagcc aagggatgat atatcccgtc ttgcaaccgg ccgcacagaa aaccgccgga gccagacagg agccagaaag cactggcgaa caattccctg cggtcaacag ccgcaaaact tggcttacaa aggcggctgg aacgttttct gtattgcaca ttgctgatat aaaatcaggg ataaactgaa gctacgggaa tggcaagcca tatcctcgtc cattatccgt aacggctaaa aaaaacagag aaataaacag aaacagcagc cgataaaaag aataacgaaa ttcactggac cgatatatac gatgtataag gctacgtacc cgcgttaggc tggtgcaatg gaacaacagg agcacgcgaa cgaaattatt aatgaaaggg 000000000000000000006 628406284062840628400 11233445667789900122 11111 <210> SEQ ID NO 17 <211>1032&Lt; 212 > DNA < 213 > Salmonella enteritidis < 400 > 16 gtgactgaac cgcagacaga gcattgcagc gtgggttttc tctgtgtcgt cgtcagaccg aaaccagagg cagagcttca agttcagcgg gatgaagcaa cttccatatg aggcgttttg agcgccagcg gccgggtctt cgcagtaagc gaagccggtg atggtcggga gacagccaga gcattttcta actctgattg ggttaa aggaaaataa aagaactgga ttggtattga ttctgcttgc caggggatga gactgagcga aagaaaaagt gccgtgcact gaacgtcagt aagcaaccgc acccaaattt tttctgacct gtaatgtaaa taaatcatgg cttttattga cttctgggtg tgataccgga ccgactatac atagtgtgaa tcggtcagga aatcccgact acaggccggg aaaacttaaa tgccggtgtt gaaacccgca agatatcgat catttcttct tgacgtttct ttcacatact acagactaca gtttatcccg ggcggggaaa acttatcgaa acaagggcgt tattcccctg gattgacact tttaagtcag ggcaaacagt tattccgcca tctggatttt gcaaccgaaa aagactccgg aagtcacgta tctgtttatt agtcagccgc ccttttaata gaaaagactg cttgatggca gcggccagtg gaatctgcgc gaagggacat ctggaatgta agaggaaccg gtgtttgtta gttgactcac catttcagtg gccgccagtg cgccaggctt acgctttata tcaggcattt ttgaacagca aagaagagcc aagggatgat Atatcccgtc ttgcaaccgg ccgcacagaa aaccgccgga gccagacagg agccagaaag cactggcgaa caattccctg cggtcaacag ccgcaaaact tggcttacaa aggcggctgg aacgttttct gtattgcaca ttgctgatat aaaatcaggg ataaactgaa gctacgggaa tggcaagcca tatcctcgtc cattatccgt aacggctaaa aaaaacagag aaataaacag aaacagcagc cgataaaaag aataacgaaa ttcactggac cgatatatac gatgtataag gctacgtacc cgcgttaggc tggtgcaatg gaacaacagg agcacgcgaa cgaaattatt aatgaaaggg 000000000000000000006 628406284062840628400 11233445667789900122 11111 < 210 > SEQ ID NO 17 < 211 >1032

<212>DNA <213>腸炎沙門氏菌 <400>17 gtgaataacg ctgaatgaga aaggtcaggg tttgcatcca cgctctgcca aaagacactg tttataaaac gataatattt cgtcagggga aaaacagaca ttgagggtgt gaaagtggca cactggccga cgcttccggg tttctataac agggatttta ctgctttaat aaacgatagt tctgatttat cacggaaatt atggtatgaa ttttcatgac tggagaacgt aatccgtaag ttcacgcgtc cacttccgga tttctgtgga gatgtggtca gctgttaacc caaaagatga ggcggttctg gttcaggttg ccgtcaggta agtggtttaa tattttgatc gggacgggtt acgattattt gaccaggaga gttacagtga tgactcctga tgatcccacc tttttatcag gtggtgactc gggttatacc caggtaaaac ttatcactgg aatatttata ttgtctttct atatcccctg ggcaactgaa tcatgacaaa ggttatggaa tgaatcactg tacctttgca ooooooooo 628406284 11233445 28 201142028 aatgcctgtc gaggcaaaat agtaaggtta attctgatga ggccatgcag attgttcagc agacgtgtac tttcgtttcg ctgaaggagt tggaatatat tcagattcca ttacctcagc cagaaatcag gaaacattac gatgttatat tgagtaatat cttccggtat ga accgcatcat taaaaaccat gagtcttctg gggggctgag caccgttcac gaatcctgaa tgatattatc ttattacaaa ctgcggtgta gtaaaacttt cgttcctgtt gcatgggatt gaaagtagtc tgtcagaatc atgagtatta caacttcatt tttctattga actatccggc ttcgtatgaa ttctgaaagc ctgaatatgc taccattcaa aataccttga ttgatgacta agatactgat agagggtgag tccggacagg atcgagttca tgtgcttggg tgtcatttta tgatgaagat tttcagaaaa 000000002 062840623 667789900 11 <210> SEQ ID NO 18 <211>615&Lt; 212 > DNA < 213 > Salmonella enteritidis < 400 > 17 gtgaataacg ctgaatgaga aaggtcaggg tttgcatcca cgctctgcca aaagacactg tttataaaac gataatattt cgtcagggga aaaacagaca ttgagggtgt gaaagtggca cactggccga cgcttccggg tttctataac agggatttta ctgctttaat aaacgatagt tctgatttat cacggaaatt atggtatgaa ttttcatgac tggagaacgt aatccgtaag ttcacgcgtc cacttccgga tttctgtgga gatgtggtca gctgttaacc caaaagatga ggcggttctg gttcaggttg ccgtcaggta agtggtttaa tattttgatc gggacgggtt acgattattt gaccaggaga gttacagtga tgactcctga tgatcccacc tttttatcag gtggtgactc gggttatacc caggtaaaac ttatcactgg aatatttata ttgtctttct atatcccctg ggcaactgaa tcatgacaaa ggttatggaa tgaatcactg tacctttgca ooooooooo 628406284 11233445 28 201142028 aatgcctgtc gaggcaaaat agtaaggtta attctgatga ggccatgcag attgttcagc agacgtgtac tttcgtttcg ctgaaggagt tggaatatat tcagattcca ttacctcagc cagaaatcag gaaacattac gatgttatat tgagtaatat cttccggtat ga accgcatcat taaaaaccat gagtcttctg gggggctgag caccgttcac gaatcctgaa tgatattatc Tttattacaaa ctgcggtgta gtaaaacttt cgttc ctgtt gcatgggatt gaaagtagtc tgtcagaatc atgagtatta caacttcatt tttctattga actatccggc ttcgtatgaa ttctgaaagc ctgaatatgc taccattcaa aataccttga ttgatgacta agatactgat agagggtgag tccggacagg atcgagttca tgtgcttggg tgtcatttta tgatgaagat tttcagaaaa 000000002 062840623 667789900 11 < 210 > SEQ ID NO 18 < 211 > 615

<212>DNA <213>腸炎沙門氏菌 <400>18 atgacgtctc gagaatttta gattgtctta cttttagcaa gataataatg gaaagagctg acgacacttg cgtaattcaa agggttcttg aatcttaaga aaagtggaga tgacgccatt tgaaaaaagt aaactcttta atgctttatt attttatcaa ctaatcgtca aaaatgaact ttgataagat ataaagatgg atcagtttga tgtaa ccgcaacgtt tcaattcaga tccagatgaa aaaaagtaaa aacaacgatg cggatggacg ggatttcttt cggtcgtaat tttctatcag gaattacatt tgcccggtga attgatgaaa ccagctttaa gctggcagta tacttaacag ttatcacgag gaccaggaac ttccattata gatgcggagc atgttatgta cctacagtcg atcagcataa cagtagctaa aggacataaa gtaaacaaag aatgtcgtta tgctgatgat tcattgttaa gtcttacaac aagggagaac gttattgtcg tgatttgctg aggcatgaaa tacgtttttt ggctgatatt ccgcatacag gaatcgttgc tgatcagacc agaaattttt tgtttcaaat&Lt; 212 > DNA < 213 > Salmonella enteritidis < 400 > 18 atgacgtctc gagaatttta gattgtctta cttttagcaa gataataatg gaaagagctg acgacacttg cgtaattcaa agggttcttg aatcttaaga aaagtggaga tgacgccatt tgaaaaaagt aaactcttta atgctttatt attttatcaa ctaatcgtca aaaatgaact ttgataagat ataaagatgg atcagtttga tgtaa ccgcaacgtt tcaattcaga tccagatgaa aaaaagtaaa aacaacgatg cggatggacg ggatttcttt cggtcgtaat tttctatcag gaattacatt tgcccggtga attgatgaaa ccagctttaa gctggcagta tacttaacag ttatcacgag gaccaggaac ttccattata gatgcggagc atgttatgta cctacagtcg atcagcataa cagtagctaa aggacataaa gtaaacaaag aatgtcgtta tgctgatgat tcattgttaa gtcttacaac aagggagaac gttattgtcg tgatttgctg aggcatgaaa tacgtttttt ggctgatatt ccgcatacag gaatcgttgc tgatcagacc agaaattttt tgtttcaaat

o o o 6 2 8 ΙΑ IX 00000005 40628401 23344566 <210> SEQ 工D NO 19 <211>1842o o o 6 2 8 ΙΑ IX 00000005 40628401 23344566 <210> SEQ Worker D NO 19 <211>1842

<212>DNA <213>腸炎沙門氏菌 ggccagtggg tttttatcgg gctgtttact tcctgaacgg ctcctgtggc gaataataac ccctctcttt tatcaggtat ctgaataaaa cggctgttgc aggaaatcga aacttctgaa tataaaggta aatacctgtg cgcgcaggta aaggtgccgc attgcgttag atccaaaaca ctgggtaata aagtttatct <400>19 gtgattatgt tgtctcgtga ctttatatat atacggacag ctcatcgtgc gacgcgaagt gaaaacgata ccagattttg cctaatcttc aaaatcacca ttcaacagta ggggctaagg gcagaagcgc catttcttta tcaatcggtt cgggaagaac gccctgacca ggttctttcc ggtaatgatt aatgcggaag gaagtgacat tttcttgatg ctttaaaact catattatac ggaaaaattt atatcaggtt ctgtttttat ttgccagtga ctgatattct tatcacttgg tggtcagaaa gtaaggtgcg aaacacacca atctgcttaa actttaataa ttaactatga ctgtcgtcga tggagggaac aaatcaggga gtaagaaaat tcgatctccg atatatcact tgcgtgaaaa agttcccttc ccccgataaa tggttctgtt tgattccatg aaccgctatc tatctggcaa taatgattta cgtcggagca cgcaggaacc acactctctg tgaaatactg gtttaatccc actgattgaa tcttgcgggt gccgtcctgg cttgtacagc aaacgggctt atatcacgaa gagcctgttt tcagttgcga ggcttacgat tcctgatttt gattggttat cttttctata attctgtttc caatactgga cttaatgagt aatattgacc gatgaaaacg acggaagatg tatctcccaa cgggaagata tttctgaacc gatcccaccc atgccaatta ttgatttaaa cgtcttatgg caggactggc tcgggcttaa gggaactgat cgttgtatca aacagcgttc ccgttcgtaa tcactgttta tgtttttcaa tgaaacatga ttaaaaaggg tctggttatg aaaaacgccg agagagtaat ggtttcgtct tctttatggt atgggagaaa gtataccgca cattggtatc ggaattgtgg gctcgaccct agcggagagt catgacaggg taagttgctt acccgttttc actcagtaag tttgcgcgat aagcattgat atgtactgat tgtcggtgtt cttcgttgtt ctgcctgatg atcagggtat oooooooooooooooooooooo 6284062840628406284062 112334456677899001223 29 201142028 attgcaggtt ttaaacttaa actgctgaca atttatcaga atatcagtca gctaaatgaa atctatggta ttgagggagc caaaacgctg atgagtgctc atccctgccg tattatctat gctgtcagcg aagaggatga tgccgcgaag atatcagaaa aacttgggta tattaccact acatcaaaga gcacaagcaa gaaccgggga cgatcaactt cacagggcga atcagaaagt gaagcccgaa gagcactggt gcttccacag gaactgggaa cgctggactt taaagaagag tttatcatcc tgaaggggga gaaccctgtt aaagcagaaa aggcacttta ttaccttgat ccgtatttta tggacaggtt aatgaaggtc agtcctaaac tggcatcatt gacgatgaaa ctaaataaga cgaaaaaaat atttggtgtg aaagggctta aatatccgtc aaaagaaaaa atgctctccg taggagagct ggagtctgag gttttgctat ga <210〉SEQ ID NO 20 <211>1167&Lt; 212 > DNA < 213 > Salmonella enteritidis ggccagtggg tttttatcgg gctgtttact tcctgaacgg ctcctgtggc gaataataac ccctctcttt tatcaggtat ctgaataaaa cggctgttgc aggaaatcga aacttctgaa tataaaggta aatacctgtg cgcgcaggta aaggtgccgc attgcgttag atccaaaaca ctgggtaata aagtttatct < 400 > 19 gtgattatgt tgtctcgtga ctttatatat atacggacag ctcatcgtgc gacgcgaagt gaaaacgata ccagattttg cctaatcttc aaaatcacca ttcaacagta ggggctaagg gcagaagcgc catttcttta tcaatcggtt cgggaagaac gccctgacca ggttctttcc ggtaatgatt aatgcggaag gaagtgacat tttcttgatg ctttaaaact catattatac ggaaaaattt atatcaggtt ctgtttttat ttgccagtga ctgatattct tatcacttgg tggtcagaaa gtaaggtgcg aaacacacca atctgcttaa actttaataa ttaactatga ctgtcgtcga tggagggaac aaatcaggga gtaagaaaat tcgatctccg atatatcact tgcgtgaaaa agttcccttc ccccgataaa tggttctgtt tgattccatg aaccgctatc tatctggcaa taatgattta cgtcggagca cgcaggaacc acactctctg tgaaatactg gtttaatccc actgattgaa tcttgcgggt gccgtcctgg cttgtacagc aaacgggctt atatcacgaa gagcctgttt tcagttgcga ggcttacgat Tcctgatttt gattgg ttat cttttctata attctgtttc caatactgga cttaatgagt aatattgacc gatgaaaacg acggaagatg tatctcccaa cgggaagata tttctgaacc gatcccaccc atgccaatta ttgatttaaa cgtcttatgg caggactggc tcgggcttaa gggaactgat cgttgtatca aacagcgttc ccgttcgtaa tcactgttta tgtttttcaa tgaaacatga ttaaaaaggg tctggttatg aaaaacgccg agagagtaat ggtttcgtct tctttatggt atgggagaaa gtataccgca cattggtatc ggaattgtgg gctcgaccct agcggagagt catgacaggg taagttgctt acccgttttc actcagtaag tttgcgcgat aagcattgat atgtactgat tgtcggtgtt cttcgttgtt ctgcctgatg atcagggtat oooooooooooooooooooooo 6284062840628406284062 112334456677899001223 29 201142028 attgcaggtt ttaaacttaa actgctgaca atttatcaga atatcagtca gctaaatgaa atctatggta ttgagggagc caaaacgctg atgagtgctc atccctgccg tattatctat gctgtcagcg aagaggatga tgccgcgaag atatcagaaa aacttgggta tattaccact acatcaaaga gcacaagcaa gaaccgggga cgatcaactt cacagggcga atcagaaagt gaagcccgaa gagcactggt gcttccacag gaactgggaa cgctggactt taaagaagag tttatcatcc tgaaggggga gaaccctgtt aaagcagaaa aggcacttta ttaccttgat ccgtatttta t Ggacaggtt aatgaaggtc agtcctaaac tggcatcatt gacgatgaaa ctaaataaga cgaaaaaaat atttggtgtg aaagggctta aatatccgtc aaaagaaaaa atgctctccg taggagagct ggagtctgag gttttgctat ga <210>SEQ ID NO 20 <211>1167

<212>DNA <213>腸炎沙門氏菌 <400>20 atgggcgttt acgttgataa agaatatcgt gttaaacgaa agtcatcaga aaatggtcgt aagtcagctt tcgctcacaa agtcaaaaat ggtggaaaga actatagccg caatgttcag gaacgtatca accgcaaggg tgccagtaag gaggttgttg tcaaaatatc tggaggtgct gttactcgtc aggggattcg gaacagtatt gattatatga gccgtgagtc agagctacca gtgatgagtg aaagcggtcg ggtatggatg ggtgccgaaa ttctggaggc taaagagcac atgatagatc gtgctaatga tcctcagcat gtgatgaatg ataaaggtaa agaaaataaa aaaatcacac agaatattgt cttctcgcct ccagttttag cgaaagtaaa gcctgaagat ttgttggagt ctgtcaggaa aacgatgcag aaaaaatatc ctaatcaccg ttttgttctt ggataccact gtgacaagaa agaacatcct cacgttcatg ttgtttttcg tatccgagat aatgacggta aacgcgctga tatcaggaaa aaagatttac gggaaattcg tacaggtttt tgtgaagagt tgaagttaaa aggttatgac gttaaagcga cccataagca acagcatgga cttaatcagt ctgttaaaga tgcacataat acagcaccaa aaagacagaa aggtgtttat gaggttgttg atattggcta tgaccattat cagaacgata aaacaaagtc taagcaacat tttataaagc taaagactct taacaagggg gttgagaaaa catactgggg ggctgatttt ggggacttat gttcgcggga aagtgttaaa gcaggtgatc ttgtcaggct gaagaaactt ggtcagaaag aagtaaaaat cccggcgctc gataaaaacg gtgttcagca tggctggaaa acggttcaca gaaatgagtg gcagttagaa aatctggggg ttaagggcgt agacagaaca ccttcagcca gcaaagagct ggtacttaac agccctgata tgctgctgaa gcaacaacag cgaatggcgc agtttacgca gcagaaagca tccacgttac agtcagaaca gaagctgaaa acagggatta agtttttggg cttataa <210> SEQ ID NO 21 <211>5655 <212>DNA <213>發光桿菌屬 <400>21 atgcctgcag atgaagcaag aggaggactc tctatgaaat ttggaaactt tttgcttaca taccaacctc cccaattttc tcaaacagag gtaatgaaac gtttggttaa attaggtcgc atctctgagg agtgtggttt tgataccgta tggttactgg agcatcattt cacggagttt ggtttgcttg gtaaccctta tgtcgctgct gcatatttac ttggcgcgac taaaaaattg aatgtaggaa ctgccgctat tgttcttccc acagcccatc cagtacgcca acttgaagat gtgaatttat tggatcaaat gtcaaaagga cgatttcggt ttggtatttg ccgagggctt tacaacaagg actttcgcgt attcggcaca gatatgaata acagtcgcgc cttagcggaa tgctggtacg ggctgataaa gaatggcatg acagagggat atatggaagc tgataatgaa catatcaagt tccataaggt aaaagtaaac cccgcggcgt atagcagagg tggcgcaccg gtttatgtgg tggctgaatc agcttcgacg actgagtggg ctgctcaatt tggcctaccg atgatattaa gttggattat aaatactaac gaaaagaaag cacaacttga gctttataat gaagtggctc aagaatatgg gcacgatatt cataatatcg accattgctt atcatatata acatctgtag atcatgactc aattaaagcg aaagagattt gccggaaatt tctggggcat tggtatgatt cttatgtgaa tgctacgact atttttgatg attcagacca aacaagaggt&Lt; 212 > DNA < 213 > Salmonella enteritidis < 400 > 20 atgggcgttt acgttgataa agaatatcgt gttaaacgaa agtcatcaga aaatggtcgt aagtcagctt tcgctcacaa agtcaaaaat ggtggaaaga actatagccg caatgttcag gaacgtatca accgcaaggg tgccagtaag gaggttgttg tcaaaatatc tggaggtgct gttactcgtc aggggattcg gaacagtatt gattatatga gccgtgagtc agagctacca gtgatgagtg aaagcggtcg ggtatggatg ggtgccgaaa ttctggaggc taaagagcac atgatagatc gtgctaatga tcctcagcat gtgatgaatg ataaaggtaa agaaaataaa aaaatcacac agaatattgt cttctcgcct ccagttttag cgaaagtaaa gcctgaagat ttgttggagt ctgtcaggaa aacgatgcag aaaaaatatc ctaatcaccg ttttgttctt ggataccact gtgacaagaa agaacatcct cacgttcatg ttgtttttcg tatccgagat aatgacggta aacgcgctga tatcaggaaa aaagatttac gggaaattcg tacaggtttt tgtgaagagt tgaagttaaa aggttatgac gttaaagcga cccataagca acagcatgga cttaatcagt ctgttaaaga tgcacataat acagcaccaa aaagacagaa aggtgtttat gaggttgttg atattggcta tgaccattat cagaacgata aaacaaagtc taagcaacat tttataaagc taaagactct taacaagggg gttgagaaaa catactgggg ggctgatttt Ggggacttat gttcgc ggga aagtgttaaa gcaggtgatc ttgtcaggct gaagaaactt ggtcagaaag aagtaaaaat cccggcgctc gataaaaacg gtgttcagca tggctggaaa acggttcaca gaaatgagtg gcagttagaa aatctggggg ttaagggcgt agacagaaca ccttcagcca gcaaagagct ggtacttaac agccctgata tgctgctgaa gcaacaacag cgaatggcgc agtttacgca gcagaaagca tccacgttac agtcagaaca gaagctgaaa acagggatta agtttttggg cttataa < 210 > SEQ ID NO 21 < 211 > 5655 < 212 > DNA < 213 > Photobacterium < 400 > 21 atgcctgcag atgaagcaag aggaggactc tctatgaaat ttggaaactt tttgcttaca taccaacctc cccaattttc tcaaacagag gtaatgaaac gtttggttaa attaggtcgc atctctgagg agtgtggttt tgataccgta tggttactgg agcatcattt cacggagttt ggtttgcttg gtaaccctta tgtcgctgct gcatatttac ttggcgcgac taaaaaattg aatgtaggaa ctgccgctat tgttcttccc acagcccatc cagtacgcca acttgaagat gtgaatttat tggatcaaat gtcaaaagga cgatttcggt ttggtatttg ccgagggctt tacaacaagg actttcgcgt Attcggcaca gatatgaata acagtcgcgc cttagcggaa tgctggtacg ggctgataaa gaatggcatg acagagggat atatggaagc tgataatgaa catatcaagt tccataaggt aaaagtaaac cccgcggcgt atagcagagg tggcgcaccg gtttatgtgg tggctgaatc agcttcgacg actgagtggg ctgctcaatt tggcctaccg atgatattaa gttggattat aaatactaac gaaaagaaag cacaacttga gctttataat gaagtggctc aagaatatgg gcacgatatt cataatatcg accattgctt atcatatata acatctgtag atcatgactc aattaaagcg aaagagattt gccggaaatt tctggggcat tggtatgatt cttatgtgaa tgctacgact atttttgatg attcagacca aacaagaggt

000000002 840628404 345566788 lx lx IX 1x 1x 1A 00000000000000000007 62840628406284062846 1123344566778990011 1111 oooooooooooooo 62840628406284 1123344566778 30 201142028 tatgatttca cgccgtattg ataattcaaa aatggaacag tttcttaaag tttggattgt cgcatgcagg gaaaatcatt ctcggtttaa cctgtcgcca ttagggttta tatcaacagc tattgtaatc tatacgccag gcggccaaaa gaatatgctg gaccatcagt acgcgtgcat aaacttgaag gctaagttgg aatgatttga cacatggaat tattaacggc tggtgataat ttgtaatgga gcaaagatgg atatatggga atgttctaaa ggatgaatgg acatctgttg aactaagaat agcgttaagt atattggccc tattgtcgct tgctgatgtg tttgacgtcc tttttctgcc gatagaaaaa aaaggcggcc ggatattcat acttggcaga tcaaaaaaat agatgcgtta tcgagttggt tcatgaaagg attcctggaa tggaaaatga aaattcatgt ttattgcgtc cgcggaatgg ggacaattga ggttaactac tagcttatgc ttaacttaag atgaattgcc gagattgtct ttgatatacc ttatcacatt gttcgcatga aagccgctat ataaagggca attacagtta aagacattga tagacgaaat aaaaacaacg tcttccttaa aaataacgga tttcagataa cagagaaaat tagcggagga gtgattgcga aactatttga cagataacga gcggacctcg aaggtattcc aaagatataa taatgatatt ttattagtga aaataattgc caattgaaaa tgagccaaaa atacctaagt caggttgaaa agtgtttacc aataacgaat aaaaatgaag tattcagaag ggcggccttt ctacctcagg gcaggtaatg cagtgtatta tttattgatg caccaaggtg tggggagggc attaaatttg gcagcgacag caaaacatat cttaatctat tattctttag caacgttgga tgtgtgtacc aagacgcaaa gcattaaaag ggatctcatg ccatctaact gaagataagt atcaaaatat ttgggaaacg tggttttgcc atttcatgtg tgaatttaca acgaaaaata aagcctatct atattctctt ggataatcta tgattttggt gtttattgct gttatcaaat cttgagtgaa cgcgatggat gtggcgtgac cgaaatcaat tgctacagga tattgcttcc ttcgctatta cttcatcaat gtatgttgat tggtgttgtc taaaattggt agcttgctta aaaaaaagat agagtgttat tttttatagc gaaatatgta tctcatcttt agccgttgcg agttaactat ttatgttctt agaaaacgct gtgtggtgcg aaatgtaatc cgaggaggat tctttcccga tgccaatatt tacggttgca aatactcaag aaatggctaa atgatgttgt atgaaagtta ttccattatc taaaaacatc tagaccctaa atacatcact cagatgcgat gttctaaaaa gtgcggctca attacatggg atgcgcatat ttcaaaaaga tgattattga ttcatcacgt ccatatctat gtgcggaaag acggaatgag atacggctaa tccttgtatt aaaaccatcg ctgccagaag cgcaggatgg atccgctatg atgtctatag aataacttcg gaaatcaatg gaaagagctt gattttgaag tgggaagatt tttactgcaa attcgtagta aatttagtgg aatgatcatc tttgtattaa cccgtgggaa atatcaaata atgaagctct tattaagtcg tcaacaactg aagttgaatt ggcgctcctc tcattaaatc ttggatcagt gaaatgcatt gaaatcatta ttccctaaaa acagcaacca aagtgggatg gataaatatg aacgaagata gaaatgcacc gtcggaaatt aaaagtgtat aatattgttg ggcaaatatg aagtgatgat gaatgactct taacattgtc acgcaggaca gctagaggcc agaaaatgaa tgttcgggct tgggatcatg gtcaaccgat tcatccgata cgcaaaagaa taattgggcg gagtctttgc tgatgtttgt aaatcattat attaccgaat aagcttgttt gtcaaatgca cgataatatt ttttccttgg gattgtagaa accgttgcaa ggatgttgcg tgtcccataa accacgttat aaaacagccc atcattttgc attcgcttca gaaagcagag gtatgttggc cttcgttttt tagggtttga gccataaatt tagcttctac ataacgataa atcgatgcaa tcctgcgcaa tggatattga aaggacataa cgccgcagga tttgttgtgg tccagtctga aggaggagaa ttcaagaaca ttgaacagat tgactgtttc acatcattac taagtgaagg tttttaatcg acgatgcttt tatctgtaaa gtcatcatat attctaatga acgttgacct gtaataaagc ctaatgaaaa atacggagtg tgctgtcctt atgataatat actaaaaaaa ttagtgcaat catgtaaaaa aattttctct tacattcgtg aattggatat cttggttctc tttccgaaag tctatattac ccttttaccg acgcgctctt attatgcgac gtagagcatg attatcgata ttttacgatc gaggaattta gccaaaaaag gctggattaa ggtgtggaat gagcaaatat gagtcatcat gcaggaatga cgattagtga gttgaaatag gtcgaggagg ttgtgttgaa aaagagaaag tggtctggcg ccacgttgga cttgttagca ttcaagctta aatcaccgca ttatctcagt gggtgctgaa aattaataac ttgggtcaag gatatattct tttttatcaa tgttgatatt agatactaat atgtattgac atttgaagct tgtcatgcca agaaatgaaa aagtatagtt tttagtgtat tggttttctg aactcatcat gagatttatt cccggttgaa aacaacaggc tccccatgct tgttgagtgg tgttagatat atcagagata taaacaagag tttcgaaaat tataactgcg tgaaccaatg taagaagtac tttcattcat ccattaattt acattattga atacggtagg acttaaaaaa ctatgatttt gccatatcat gtaaatctgt gcgcaatttt ctaatgcatt tatctgttat atgcggatgt cgccatctta atcctgttga agcgagcttg agttagcgtt attttgatga aagtagaggt ttaatcaacc tgccttatgt ttaaatatcg ataacatatt catatatttc aacagactcg agtaaaagta ggaaataaaa aatgccatta gaatatttat ttgagttcag gtggttgatt tctgcgcgga gtcggtgttg ctacccatta gtctttgcga atgatgtatc caagatgaag ttgttaggaa tcggttacga actgaaccgt oooooooooooooooooooooooooooooooo 06284062840628406284062840628406 99001223345566788990112234455677 1X IX 1L 1x 1i 1i IX IX 1x 1x οώ οώ οώ οώ οώ οώ 2820 2880 2940 3000 3060 ooooooooooooooooooooooo 28406284062840628406284 11233445667789900122334 33333333333333344444444 31 201142028 catttgaaca tttaactatt gcgacagtca atgaacgccg aatgagaatt gagattgaaa atcaagcaat ttctctgtct taagtcgagg aggaaaacag gtatgacttc atatgttgat aaacaagaaa ttacagcaag ctcagaaatt gatgatttga ttttttcgag cgatccatta gtgtggtctt acgacgagca ggaaaaaatc agaaagaaac ttgtgcttga tgcatttcgt aatcattata aacattgtcg agaatatcgt cactactgtc aggcacacaa agtagatgac aatattacgg aaattgatga catacctgta ttcccaacat cggtttttaa gtttactcgc ttattaactt ctcaggaaaa cgagattgaa agttggttta ccagtagcgg cacgaatggt ttaaaaagtc aggtggcgcg tgacagatta agtattgaga gactcttagg ctctgtgagt tatggcatga aatatgttgg tagttggttt gatcatcaaa tagaattagt caatttggga ccagatagat ttaatgctca taatatttgg tttaaatatg ttatgagttt ggtggaattg ttatatccta cgacatttac cgtaacagaa gaacgaatag attttgttaa aacattgaat agtcttgaac gaataaaaaa tcaagggaaa gatctttgtc ttattggttc gccatacttt atttatttac tctgccatta tatgaaagat aaaaaaatct cattttctgg agataaaagc ctttatatca taaccggagg cggctggaaa agttacgaaa aagaatctct gaaacgtgat gatttcaatc atcttttatt tgatactttc aatctcagtg atattagtca gatccgagat atatttaatc aagttgaact caacacttgt ttctttgagg atgaaatgca gcgtaaacat gttccgccgt gggtatatgc gcgagcgctt gatcctgaaa cgttgaaacc tgtacctgat ggaacgccgg ggttgatgag ttatatggat gcgtcagcaa ccagttatcc agcatttatt gttaccgatg atgtcgggat aattagcaga gaatatggta agtatcccgg cgtgctcgtt gaaattttac gtcgcgtcaa tacgaggacg cagaaagggt gtgctttaag cttaaccgaa gcgtttgata gttaa <210> SEQ ID NO 22 <211>101 <212>DNA <213〉大腸桿菌 <400>22 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat t 000000000000000000005 062840628406284062845 556678899011223445566 444444444555555555555 6001 32000000002 840628404 345566788 lx lx IX 1x 1x 1A 00000000000000000007 62840628406284062846 1123344566778990011 1111 oooooooooooooo 62840628406284 1123344566778 30 201142028 tatgatttca cgccgtattg ataattcaaa aatggaacag tttcttaaag cgcatgcagg gaaaatcatt ctcggtttaa cctgtcgcca ttagggttta tatcaacagc tattgtaatc tatacgccag gcggccaaaa gaatatgctg gaccatcagt aaacttgaag gctaagttgg aatgatttga cacatggaat tattaacggc tggtgataat ttgtaatgga gcaaagatgg atatatggga atgttctaaa ggatgaatgg acatctgttg acgcgtgcat tttggattgt aactaagaat agcgttaagt atattggccc tattgtcgct tgctgatgtg tttgacgtcc tttttctgcc gatagaaaaa aaaggcggcc ggatattcat acttggcaga tcaaaaaaat agatgcgtta tcgagttggt tcatgaaagg attcctggaa tggaaaatga aaattcatgt ttattgcgtc cgcggaatgg ggacaattga ggttaactac tagcttatgc ttaacttaag atgaattgcc gagattgtct ttgatatacc ttatcacatt gttcgcatga aagccgctat ataaagggca attacagtta aagacattga tagacgaaat aaaaacaacg tcttccttaa aaataacgga tttcagataa cagagaaaat tagcggagga gtgattgcga aactatttga cagataacga gcggacctcg aaggtattcc aaa gatataa taatgatatt ttattagtga aaataattgc caattgaaaa tgagccaaaa atacctaagt caggttgaaa agtgtttacc aataacgaat aaaaatgaag tattcagaag ggcggccttt ctacctcagg gcaggtaatg cagtgtatta tttattgatg caccaaggtg tggggagggc attaaatttg gcagcgacag caaaacatat cttaatctat tattctttag caacgttgga tgtgtgtacc aagacgcaaa gcattaaaag ggatctcatg ccatctaact gaagataagt atcaaaatat ttgggaaacg tggttttgcc atttcatgtg tgaatttaca acgaaaaata aagcctatct atattctctt ggataatcta tgattttggt gtttattgct gttatcaaat cttgagtgaa cgcgatggat gtggcgtgac cgaaatcaat tgctacagga tattgcttcc ttcgctatta cttcatcaat tggtgttgtc taaaattggt agcttgctta aaaaaaagat agagtgttat tttttatagc gaaatatgta tctcatcttt agccgttgcg agttaactat ttatgttctt agaaaacgct gtgtggtgcg aaatgtaatc cgaggaggat tctttcccga tgccaatatt tacggttgca aatactcaag aaatggctaa atgatgttgt atgaaagtta ttccattatc taaaaacatc tagaccctaa atacatcact cagatgcgat gttctaaaaa gtgcggctca attacatggg atgcgcatat ttcaaaaaga tgattattga ttcatcacgt ccatatctat gtgcggaaag acggaatgag atacggctaa tccttgtatt gtatgttgat aa aaccatcg ctgccagaag cgcaggatgg atccgctatg atgtctatag aataacttcg gaaatcaatg gaaagagctt gattttgaag tgggaagatt tttactgcaa attcgtagta aatttagtgg aatgatcatc tttgtattaa cccgtgggaa atatcaaata atgaagctct tattaagtcg tcaacaactg aagttgaatt ggcgctcctc tcattaaatc ttggatcagt gaaatgcatt gaaatcatta ttccctaaaa acagcaacca aagtgggatg gataaatatg aacgaagata gaaatgcacc gtcggaaatt aaaagtgtat aatattgttg ggcaaatatg aagtgatgat gaatgactct taacattgtc acgcaggaca gctagaggcc agaaaatgaa tgttcgggct tgggatcatg gtcaaccgat tcatccgata cgcaaaagaa taattgggcg gagtctttgc tgatgtttgt aaatcattat attaccgaat aagcttgttt gtcaaatgca cgataatatt ttttccttgg gattgtagaa accgttgcaa ggatgttgcg tgtcccataa accacgttat aaaacagccc atcattttgc attcgcttca gaaagcagag gtatgttggc cttcgttttt tagggtttga gccataaatt tagcttctac ataacgataa atcgatgcaa tcctgcgcaa tggatattga aaggacataa cgccgcagga tttgttgtgg tccagtctga aggaggagaa ttcaagaaca ttgaacagat tgactgtttc acatcattac taagtgaagg tttttaatcg acgatgcttt tatctgtaaa gtcatcatat attctaatga acgttgacct gtaataaagc c taatgaaaa atacggagtg tgctgtcctt atgataatat actaaaaaaa ttagtgcaat catgtaaaaa aattttctct tacattcgtg aattggatat cttggttctc tttccgaaag tctatattac ccttttaccg acgcgctctt attatgcgac gtagagcatg attatcgata ttttacgatc gaggaattta gccaaaaaag gctggattaa ggtgtggaat gagcaaatat gagtcatcat gcaggaatga cgattagtga gttgaaatag gtcgaggagg ttgtgttgaa aaagagaaag tggtctggcg ccacgttgga cttgttagca ttcaagctta aatcaccgca ttatctcagt gggtgctgaa aattaataac ttgggtcaag gatatattct tttttatcaa tgttgatatt agatactaat atgtattgac atttgaagct tgtcatgcca agaaatgaaa aagtatagtt tttagtgtat tggttttctg aactcatcat gagatttatt cccggttgaa aacaacaggc tccccatgct tgttgagtgg tgttagatat atcagagata taaacaagag tttcgaaaat tataactgcg tgaaccaatg taagaagtac tttcattcat ccattaattt acattattga atacggtagg acttaaaaaa ctatgatttt gccatatcat gtaaatctgt gcgcaatttt ctaatgcatt tatctgttat atgcggatgt cgccatctta atcctgttga agcgagcttg agttagcgtt attttgatga aagtagaggt ttaatcaacc tgccttatgt ttaaatatcg ataacatatt catatatttc aacagactcg agtaaaagta ggaaataaaa aatgccatta gaatatttat ttgagttcag gtggttgatt tctgcgcgga gtcggtgttg ctacccatta gtctttgcga atgatgtatc caagatgaag ttgttaggaa tcggttacga actgaaccgt oooooooooooooooooooooooooooooooo 06284062840628406284062840628406 99001223345566788990112234455677 1X IX 1L 1x 1i 1i IX IX 1x 1x οώ οώ οώ οώ οώ οώ 2820 2880 2940 3000 3060 ooooooooooooooooooooooo 28406284062840628406284 11233445667789900122334 33333333333333344444444 31 201142028 catttgaaca tttaactatt gcgacagtca atgaacgccg aatgagaatt gagattgaaa atcaagcaat ttctctgtct taagtcgagg aggaaaacag gtatgacttc atatgttgat aaacaagaaa ttacagcaag ctcagaaatt gatgatttga ttttttcgag cgatccatta gtgtggtctt acgacgagca ggaaaaaatc agaaagaaac ttgtgcttga tgcatttcgt aatcattata aacattgtcg agaatatcgt cactactgtc aggcacacaa agtagatgac aatattacgg aaattgatga catacctgta ttcccaacat cggtttttaa gtttactcgc ttattaactt ctcaggaaaa cgagattgaa agttggttta ccagtagcgg cacgaatggt ttaaaaagtc aggtggcgcg tgacagatta agtattgaga gactcttagg ctctgtgagt tatggcatga aatatgttgg tagttggttt gatcatcaaa tag aattagt caatttggga ccagatagat ttaatgctca taatatttgg tttaaatatg ttatgagttt ggtggaattg ttatatccta cgacatttac cgtaacagaa gaacgaatag attttgttaa aacattgaat agtcttgaac gaataaaaaa tcaagggaaa gatctttgtc ttattggttc gccatacttt atttatttac tctgccatta tatgaaagat aaaaaaatct cattttctgg agataaaagc ctttatatca taaccggagg cggctggaaa agttacgaaa aagaatctct gaaacgtgat gatttcaatc atcttttatt tgatactttc aatctcagtg atattagtca gatccgagat atatttaatc aagttgaact caacacttgt ttctttgagg atgaaatgca gcgtaaacat gttccgccgt gggtatatgc gcgagcgctt gatcctgaaa cgttgaaacc tgtacctgat ggaacgccgg ggttgatgag ttatatggat gcgtcagcaa ccagttatcc agcatttatt gttaccgatg atgtcgggat aattagcaga gaatatggta agtatcccgg cgtgctcgtt gaaattttac gtcgcgtcaa tacgaggacg cagaaagggt gtgctttaag cttaaccgaa gcgtttgata gttaa < 210 > SEQ ID NO 22 < 211 > 101 < 212 > DNA < 213> Escherichia coli < 400 > 22 aacgcaatta Atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat t 000000000000000000005 062840628406 284062845 556678899011223445566 444444444555555555555 6001 32

Claims

201142028 VII. Patent application scope: .- Stable spontaneous luminescent plastids for Gram-negative fine g, which contains at least one spontaneous luminescent gene, at least one promoter capable of initiating spontaneous luminescent genes, at least _ c〇iei replicon , gene, to gene, at least one resistance gene, at least one gene, at least one less than one / / / gene, at least one μ (10) gene and at least _

And comprising at least one gene, at least one gene, at least one gene, at least one gene, at least one gene, at least one gene, at least one gene, at least one J gene, at least one gene, at least one eve, and at least one J77 gene The gene has at least one bΜ gene and at least one gene. 2. The spontaneous luminescent plastid of a Gram-negative bacterium as described in claim 1, wherein the promoter capable of activating the spontaneous luminescent gene is a PlaeZ promoter. 3. The spontaneous luminescent plastid of a Gram-negative bacterium as described in claim 1 or 2, wherein the resistance gene comprises a resistance gene to ampicillin. 4. The spontaneous luminescent plastid of Gram-negative bacteria as described in claim 1 or 2 wherein the resistance gene comprises a resistance gene to kanamycin. 5. The spontaneous luminescent plastid of a Gram-negative bacterium as described in claim 1 or 2, wherein the resistance gene comprises a resistance gene to ampicillin 13 201142028 (ampicillin) and kanamycin (kanamycin) anti-sex gene. 6. The spontaneous luminescent plastid of the Gram-negative bacterium according to claim 1, wherein the stable spontaneous luminescent substance specific to the Gram-negative bacterium is the nucleotide sequence as set forth in SEQ ID NO: 1. 7. A method for preparing a stable spontaneous luminescent vector for Gram-negative bacteria, comprising the steps of: (a) ligating a luminescent gene lacking a promoter into a plastid containing a promoter, thereby enabling a luminescent gene ( Promoting transcription using a promoter; (b) inserting a Gram-negative bacterial plastid containing at least one Col E1 replicon and at least one resistance gene into the plastid pSE34; (c) constructing step (a) The plastid is inserted into the plastid constructed in the step (b) to obtain a stable spontaneous luminescent carrier for the gram-negative bacteria. 8. Preparation of Gram-negative bacteria as described in claim 7 of the patent application _ A method for stabilizing a spontaneous luminescent vector, wherein the promoter of the step (a) is a PlaeZ promoter. 9. A method for preparing a stable spontaneous luminescent vector for gram-negative bacteria according to claim 8 of the patent application, The promoter-containing plastid described in the step (a) may be a plastid pGEM3_Zf +. The method for preparing a stable spontaneous luminescent carrier for Gram-negative bacteria according to claim 9 of the invention, wherein the step (3) stated 14 201142028 The luminescent gene (luxABCDE) of the spent promoter is derived from the plastid 5. 1 1. The method for preparing a stable spontaneous luminescent vector for Gram-negative bacteria according to the scope of claim 7 of the patent, wherein step (b) The gram-negative bacterial plastid of at least the -c〇m replicon and the at least one resistance gene may be the plastid pBlueScript II KS (+ /-). 1 2 - the plastid of the Gram-negative bacteria, The plastid can be stably distributed in the bacterium when the bacterial cell divides, and the plastid can be spread by the zygosity between the bacterium and the other bacteria, and the plastid contains at least one p/r gene, at least one a % gene, at least one μ gene, at least one S Ζ / / gene and at least one $ (from the gene, and comprising at least one P ///gene, at least one Μ7 gene, at least one gene, at least one P7/ J5 gene, at least one 7/no gene, at least one eve/J7 gene, at least one gene, at least one gene, at least one gene to Ρ·?/ especially//gene, at least one fah gene, at least one taxB gene With at least one gene. 1 3 · as applied Gram body mass of the item 12 negative bacteria of interest range 'wherein the plasmid is as SEQ ID N0: 2 nucleotide sequence of the 15