CN102268447B - Stably self-luminous bacteria plasmid and preparation method thereof - Google Patents

Abstract

The invention relates to a stably self-luminous bacteria plasmid and a preparation method thereof. The stably self-luminous bacteria plasmid comprises a luxABCDE self-luminous gene, a promoter capable of starting the luxABCDE self-luminous gene, a ColE1 replicon, an ampicillin resistant gene, a kanamycin resistant gene, stable plasmid correlated genes and conjugation effect correlated genes. The stably self-luminous bacteria plasmid of the invention can easily enter into bacteria through conjugational transfer to express a luminous gene protein and stably stay in bacteria in quantity. Besides, the stably self-luminous bacteria plasmid can be luminous continuously and efficiently without addition of extra luminous substrates or screening of antibiotic, so as to facilitate direct observation of bacteria dynamic change in an animal in vivo. The preparation method of the invention comprises the following steps of: a. acquiring a plasmid capable of starting a luminous gene; b. inserting a plasmid containing the ColE1 replicon and the resistant gene into a plasmid pSE34; c. combining the plasmids from step a. and step b. to obtain the plasmid of the invention.

Description

Can stablize self luminous bacterial plasmid and preparation method thereof

Technical field

The present invention is a kind of can be used for luminous plasmid of demarcating bacterium and preparation method thereof, espespecially technical provide a kind of can stablize in bacterium breeds from generation to generation and be present in bacterium in a large number in the spontaneous luminescence carrier and preparation method thereof of gram-negative bacteria.

Background technology

Generally wishing to get and know distribution and the behavior of bacterium in animal body, is mostly after laboratory animal is sacrificed, ability analyzing animal organ sample, and can not direct viewing.

So someone researches and develops the method for utilizing bioluminescence gene to show in bacterium then, observe distribution and the behavior of bacterium in animal body, and the method for utilizing bioluminescence gene to show there are three kinds of main paties in general in bacterium:

1. directly show bioluminescence gene in plasmid, but be subject to the unstable of plasmid itself, so that most bacterium is easily lost the plasmid of implanting, can't stablize and a large amount of existing in bacterium, and in the breeding of bacterium, plasmid is difficult to stable each careful bacterium that is present in, and often can only keep several generations, therefore the intensity of whole bacterium performance is very fast namely turn a little less than.If the use antibiotic-screening forces bacterium to possess this plasmid, otherwise can't survive, but this method can't apply in living animal, because if give the feeding animal microbiotic, by microbiotic to the caused change of test animal because of may complicated experimental result.

2. meet machine ground to insert in the karyomit(e) of bacterium by transposon (transposon) bioluminescence gene, but can not determine whether the DNA place that is inserted into is important gene place; Because the transposition Probability is not high, more increase the degree of difficulty of filling out in screening again.Moreover only for the bacterial strain of this kind transposition, the position of its transposon will be different if be applied to other kind bacterial strain for this method, so will more increase fill out change because of so that can not do observation and the comparison of multiple bacterial strain.

3. with dual group of exchange, bioluminescence gene is inserted in the karyomit(e) of bacterium, although can insert exactly in specific gene, but its Probability is not high, again because of bioluminescence gene (luxABCDE-kan) itself very long (7kb at least), more reduce the probability of restructuring exchange, be difficult to complete clone and the transmission of macromole DNA in bacterium.

Summary of the invention

The object of the invention is to, a kind of self luminous bacterial plasmid and preparation method thereof of stablizing is provided, the technical problem point that wish solves is: generally wish to get and know distribution and the behavior of bacterium in animal body, mostly need to sacrifice animal ability analyzing animal organ sample, and the method that the general using bioluminescence gene shows in bacterium, following some disappearance is arranged: if 1. directly show bioluminescence gene in plasmid, be subject to the unstable of plasmid itself, most bacterium is easily lost the plasmid of implanting, and can't stablize and a large amount of existing in bacterium; 2. if bioluminescence gene meets machine ground to insert the karyomit(e) of bacterium by transposon (transposon), can not determine that whether be inserted into the DNA place is important gene place, and the transposition Probability is not high; The test strain that also only is subject in addition this transposition can be used, and but can not be widely used on other multiple bacterial strain; 3. if with dual group of exchange, bioluminescence gene is inserted in the karyomit(e) of bacterium, its Probability is not high, and very long clone and the transmission that is not easy to complete in bacterium of bioluminescence gene itself.

The technical characterstic of dealing with problems: a kind ofly stablize self luminous bacterial plasmid, it comprises luxABCDE spontaneous luminescence gene, the promotor that can start luxABCDE spontaneous luminescence gene, ColE1 replicon, the resistant gene to Ampicillin Trihydrate and kantlex, pir, parG, parF, stbD, and the genes involved of stbE stable plasmid, and pilX1 to pilX11 and taxA, taxB, gene that taxC zygogamy effect is relevant; The following a. acquisition one of plasmid preparation process of the present invention can start the plasmid of bioluminescence gene (luxABCDE); B. will contain in the plasmid access plasmid pSE34 of ColE1 replicon and resistant gene; C. integrating step a. and b. plasmid make plasmid of the present invention.

The effect of contrast prior art: the stable spontaneous luminescence carrier of gram-negative bacteria special use of the present invention has following some effect:

The first, can directly use this luminous plasmid in bacterium, be conducive to observe its changes in distribution in animal body.Thereby need not to sacrifice animal, can distribution and the behavior in animal body of direct analysis research bacterium.

The second, but luminous plasmid stable existence of the present invention in bacterium, but and the massive duplication plasmid, the luminous intensity that so helps bacterium, can continue and can show efficiently, thereby can overcome plasmid in the breeding of bacterium, being difficult to stable and a large amount of shortcomings that exist in bacterium.

The 3rd, the present invention can utilize the zygogamy effect of plasmid, not only can help clone (cloning) but also can be convenient to this plasmid to transmit luminous plasmid of the present invention between bacterium and bacterium.So can simply select needed high-effect and lasting luminous bacterium, and can reduce many burdens at clone's screening operation when (cloning), especially the present invention is excessive because of the gene so that the plasmid DNA that contain many critical functions, so when in general dna clone's (cloning) montage, probalility of success is gone up very low relatively.Therefore, utilize the zygogamy effect can reduce degree of difficulty on the clone.

Description of drawings

Fig. 1: the flow chart of steps of one embodiment of the present invention.

Fig. 2 A: the plasmid pXen-5 Organization Chart of one embodiment of the present invention.

Fig. 2 B: one embodiment of the present invention constructs plasmid p3ZLux4 schematic diagram.

Fig. 3 A: the plasmid pSE34 Organization Chart of one embodiment of the present invention.

Fig. 3 B: one embodiment of the present invention constructs plasmid pSE-Lux1 schematic diagram.

Fig. 4: be the plasmid pSE-Lux1 Organization Chart of one embodiment of the present invention.

Embodiment

the self luminous bacterial plasmid pSE-Lux1 (SEQ ID NO 1) that stablizes of the present invention contains at least one luxABCDE spontaneous luminescence gene (SEQ ID NO 21), at least one promotor that starts luxABCDE spontaneous luminescence gene, but the ColE1 replicon of at least one massive duplication, at least one resistant gene, at least one pir gene (SEQ ID NO 3), at least one parG gene (SEQ ID NO 4), at least one parF gene (SEQ ID NO5), at least one stbD gene (SEQ ID NO 6) and at least one stbE gene (SEQ ID NO 7), and comprise at least one pilX1 gene (SEQ ID NO 8), at least one pilX2 gene (SEQ ID NO 9), at least one pilX4 gene (SEQ ID NO 10), at least one pilX5 gene (SEQ ID NO 11), at least one pilX6 gene (SEQ ID NO 12), at least one pilX7 gene (SEQ ID NO 13), at least one pilX8 gene (SEQID NO 14), at least one pilX9 gene (SEQ ID NO 15), at least one pilX10 gene (SEQ ID NO 16), at least one pilX11 gene (SEQ ID NO 17), at least one taxA gene (SEQ ID NO 18), at least one taxB gene (SEQ ID NO 19) and at least one taxC gene (SEQ ID NO 20).

Wherein, this promotor that can start luxABCDE spontaneous luminescence gene is P _LacZPromotor (SEQ ID NO22).

Wherein, this resistant gene can be Ampicillin Trihydrate (ampicillin, Amp) resistant gene or to kantlex (kanamycin, Kan) resistant gene, or contain Ampicillin Trihydrate (ampicillin, Amp) resistant gene adds the two kinds of resistant genes of resistant gene to kantlex (kanamycin, Kan).

Wherein, this pir, parG, parF, stbD and stbE are the genes involved of stable plasmid; This pilX1, pilX2, pilX4, pilX5, pilX6, pilX7, pilX8, pilX9, pilX10, pilX11, taxA, taxB are the relevant gene of zygogamy to the taxC gene, help the zygogamy effect of plasmid, help the clone of plasmid and the transmission (as plasmid is passed to intestinal bacteria from Salmonellas) between bacterium and other bacterial classification bacteriums.

Wherein, luxABCDE spontaneous luminescence gene not only contains Luminescence Enzyme luxAB gene but also lipid acid reductase enzyme luxCDE gene is arranged, can synthetic fat aldehyde, directly for Luminescence Enzyme required matter that is subjected to when luminous, therefore need not additionally to be subjected to dosing of matter (as fatty aldehydes).Plasmid pSE-Lux1 of the present invention can show the spontaneous luminescence gene protein, and it is luminous that again can be highly stable exist in bacterium continues, and in can stablizing in bacterium breeds from generation to generation and being present in bacterium in a large number, more is conducive to direct viewing in living animal.

It is of the present invention that to stablize self luminous bacterial plasmid preparation process as follows:

(a), bioluminescence gene (luxABCDE) access one that will lack promotor contains in the plasmid of promotor, make bioluminescence gene (luxABCDE) can utilize promotor to start and transcribe.

(b), will contain in the gram-negative bacteria plasmid access plasmid pSE34 of at least one ColE1 replicon and at least one resistant gene.

(c), the plasmid that step (a) is constructed accesses in the constructed plasmid of step (b) and can stablize self luminous bacterial plasmid to make this.

Embodiment

The detailed preparation process that reality of the present invention can be stablized self luminous bacterial plasmid in preparation is as follows:

(a), construct plasmid p3ZLux4 (10): the bioluminescence gene (luxABCDE) that will lack promotor cuts out with the PstI restriction enzyme from plasmid pXen-5, and access contains P _LacZIn the plasmid pGEM 3-Zf+ of promotor, make bioluminescence gene (luxABCDE) can utilize P in plasmid pGEM 3-Zf+ _LacZPromotor starts transcribes.

Wherein, plasmid pXen-5 is the commercial plasmid (Alameda, CA, USA) of Xenogen company, and 18,357 base pairs (bp) are arranged.Contain the resistant gene of kantlex (kanamycin, Kan) and the spontaneous luminescence gene of luxABCDE.Start luxABCED but lack promotor, so that can not start bioluminescence gene.Wherein luxABCDE not only contains Luminescence Enzyme luxAB gene but also contain lipid acid reductase enzyme luxCDE gene, lipid acid can be reduced into alkanoic, and Luminescence Enzyme required matter that is subjected to when luminous directly is provided, and therefore need not additionally to dose other as the matter that is subjected to of fatty aldehydes.

Wherein, plasmid pGEM 3-Zf+ is the commercial plasmid (Madison, WI, USA) of Promega company, and 3,199bp is arranged.But the ColE1 replicon, the multiple clone site that contain massive duplication reach Ampicillin Trihydrate (ampicillin, Amp) resistant gene, but belong to the ColE1 type replicon of narrow host range, and be unsettled plasmid, easily omit plasmid in bacterium after number generation breeding.

Wherein, the constructed plasmid p3ZLux4 of step (a) has 11,766bp, but is the luminous plasmid of massive duplication, but itself is unstable.

(b), construct plasmid pBS-SE34 (11): plasmid pBlueScript II KS (+/-) is cut out and accesses in plasmid pSE34 with the XbaI restriction enzyme and plasmid.

Wherein, plasmid pBlueScript II KS (+/-) is the commercial carrier (LaJolla, CA, USA) of Stratagene company.But the ColE1 replicon, the multiple clone site that contain massive duplication reach the resistant gene to Ampicillin Trihydrate (ampicillin, Amp).

Wherein, plasmid pSE34 (SEQ ID NO 2) has 32,950kb, and from a kind of stable primary type Salmonella enteritidis (Salmonella enterica serotype Enteritidis) of bacterium in intestines, its bacteriophage typing is PT34.Salmonella enteritidis is the main pathogeny that (comprises Taiwan) in the world and cause animal and people's Salmonellas associated conditions, and wherein bacteriophage typing PT34 is the general Salmonella enteritidis in Taiwan second.This bacteriophage typing PT34 finds it is Salmonella enteritidis from the most general bacteriophage typing PT4 through the research of the applicant research team, and the Salmonella enteritidis of bacteriophage typing PT4 becomes bacteriophage typing PT34 because change after obtaining plasmid pSE34.The applicant research team finds that also this plasmid has pir, parG, parF, stbD, reaches the stbE gene, and when helping the cell proliferation division, plasmid can stably be allocated in careful bacterium.And contain pilX1, pilX2, pilX4, pilX5, pilX6, pilX7, pilX8, pilX9, pilX10, pilX11, taxA, taxB and taxC gene and can help plasmid to scatter with zygogamy effect (conjugation) between bacterium and other bacterial classification bacteriums.

Wherein, the constructed plasmid pBS-SE34 of step (b) has 34.6kb, has advantages of plasmid pSE34, the genes involved genetic characteristics relevant with the zygogamy effect that contains stable plasmid, and has advantages of plasmid pBlueScript II KS, but the ColE1 replicon, the multiple clone site that contain massive duplication reach the resistant gene to Ampicillin Trihydrate (ampicillin, Amp).

(c), construct plasmid pSE-Lux1 (12): utilize SalI restriction enzyme and XhoI restriction enzyme to cut respectively the constructed plasmid p3ZLux4 of (a) step and the constructed plasmid pBS-SE34 of (b) step, again plasmid p3ZLux4 and plasmid pBS-SE34 are bonded into a plasmid pSE-Lux1, this plasmid pSE-Lux1 has 46.3kb, is the stable spontaneous luminescence carrier of a gram-negative bacteria special use.

Function test

see also following table one, be plasmid pSE-Lux1 stability test of the present invention, we implant plasmid p3Zlux4 in clone strain intestinal bacteria (E.coli) and are used as control group, plasmid pSE-Lux1 is implanted in Salmonellas (S.Typhimurium LBNP4417) be used as experimental group, after cultivating for 79 generations respectively in the Luria-Bertani that does not contain antibiotic-screening (LB) liquid nutrient medium, this bacterium liquid is grown in being coated with respectively under different extension rates to coil in not containing microbiotic and containing in LB agar glue (containing 1.5% the agar glue) culture dish of microbiotic (as ampicillin and kanamycin), rear calculating total bacteria overnight and contain bacterial count (the colony formationunit of plasmid, CFU), the plasmid pSE-Lux1 that can clearly find this creation can be after 79 generations, still have 75% (1550/2030) bacterium still to have luminous plasmid, bacterium is after the succeeding transfer culture in four days (approximately 79 generations), the luminous plasmid pSE-Lux1 that finds this creation exists the stabilization efficiency in bacterium to be better than approximately 100,000 times of plasmid p3ZLux4, observe the luminous situation of bacterium colony on culture dish, the illumination effect that plasmid p3ZLux4 produces approximately 8 hours, and plasmid pSE-Lux1 is luminous more than at least 2 days.This result shows that the constructed plasmid pSE-Lux1 of the present invention both can show the spontaneous luminescence gene protein, and again can be highly stable exist in bacterium is lasting luminous.

Table one, plasmid pSE-Lux1 stability test

Sequence table

<160>22

<210>1

<211>46313

<212>DNA

＜213〉artificial sequence

<220>

<223>

<400>1

tgtcactgat ccaacgatta aaaaattact gaaagataag gataaagaca aaaaagatga 60

acatggcggt attggtacgc cagctacccg tgcagccatt ctggaaacgc tgaagaagag 120

aaactatatc acgctggaaa aagggaaact tattccgact gataccggat atgcgcttat 180

tgatgccctg ccaggtatag cggttaatcc tgatatgaca gcattatggt ctgaaaagca 240

gactgccatt gaaaatggcg acctgacggt tgaacagttt attaatgagc tgtacggtga 300

attgacaggc atgatttctg atgttgacct gggcaagatg aagattgaac ccgctgcgcc 360

agcagggcag tttcaacgcc tggactctcc ctgcccttcc tgtggtaaac atattgttat 420

caggccgaaa ggttatttct gtaccggatg tgaatttaaa atctggagtg agttttctgg 480

taagaaaatc acccaggcac aggccgaaaa actggttaaa tcagggaaaa ccgatttgat 540

taagggattt aaaaagaaaa gtggtggaac gtatgataca gttcttgtcc ttgaggataa 600

gaaaacaggg aagctgggtt ttccggcaag ggctaagaag tgaaaacaaa gcaggaatgg 660

ctttttcagt taagaaaatg tacatcaaga gatactcttg aaaaagttat tgagattaac 720

cgttacaagc tgcctttatc agaatcagag gcattttatt ctgccgcaga tcaccgccgt 780

gcagaactgg tgatgaataa actttatgat aaggttcctt ccggcgtatg gaagtacgtc 840

cattaaacaa gaggattaat tatgagcgaa ctgactaaag aagatgaata cggcattatc 900

agccggacta tgatgaatat tcgttcattg cgtgtgtttg cccgtgagat tgattttgag 960

cagttgctcg aaatgcagga aaagctcaac gttgttattg aagaacgtcg tgaagatgct 1020

gaacgtgaag cggctgaacg agcagagcgt gaacggaaac gtcaggaact gcttcagtta 1080

atcgccggag agggtttctc accggaagaa ctgcttggtc tgtccgaaga agcaccaaaa 1140

tcacgtaaaa aaacgttacc aaaagcgcca cccaagtatc aatttgaaga aaatggtgaa 1200

acgaaatact ggtctggtcg tggacgtgcg ccaaaaccga ttgatgaagc gttgaaagcc 1260

gggcgttctc tggaagattt tcgtatcaat aagagtttga acggagtaac agatgagcag 1320

taatatggca aggatatagt tttatatcat cattttgtta aggaagaaaa tccatgagta 1380

atacatccta caaacaaatt atccctgcga cagactggta tttccgtcac gataatgtct 1440

ccggtgtggc aggaaagtca acagtatacc aactggctgc atgggcgctt aaagaaaatg 1500

gtgaggtagt tggtctggtg acggttcgtg atgataatgg gcgtcctaaa ctggttactc 1560

ctccccctgt ccctggtgat tatttgcata aagaacaact caccgatgat gaaaaagagt 1620

gggcgaagag acgctaaact atattcatat aaagcctctg ttctagagcg gccgccaccg 1680

cggtggagct ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt 1740

ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat 1800

ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag 1860

ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt 1920

taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt 1980

ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc 2040

cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg 2100

gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac 2160

taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg 2220

tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag 2280

cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt 2340

caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 2400

attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 2460

aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 2520

tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 2580

agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 2640

gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 2700

cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 2760

agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 2820

taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 2880

tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 2940

taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 3000

acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 3060

ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 3120

cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 3180

agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 3240

tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 3300

agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 3360

tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 3420

ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 3480

tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 3540

aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 3600

tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 3660

agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 3720

taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 3780

caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 3840

agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 3900

aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 3960

gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 4020

tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 4080

gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 4140

ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 4200

ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 4260

aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 4320

aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 4380

atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 4440

tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 4500

acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctgggtacc gggccccccc 4560

tcgacaagct caagggattc aagtcgccaa agaaaaaggt atatataaag ggagacctgt 4620

tctgtattct cccaatgcta aagacccaca aaagcgttta gtttattacc gagttgttga 4680

attacttgaa cagggtaaat ctataagtac tatagctaaa gaagttggta ttacacgtca 4740

aactatatat agaataaaaa atagtaaata aaataacata gacaataatc tatatagatg 4800

ttaatctatt taacatctag gaggtttatt catgtcttat aaagaactat caacaatatt 4860

aaaaatttta tcagattcaa gtaggttaga aatattagat ttactttctt gtggtgagct 4920

atgcgcttgt gacttattag aacactttca attctcacaa cctacactaa gtcatcatat 4980

gaagtcatta gtagataatg aattagttac aacacgaaaa gacggcaata aacattggta 5040

tcaacttaat catgctattt tagatgatat tatccaaaac ttgaacatca ttaatacatc 5100

taatcaaaga tgtgtatgta aaaatgtgaa atcaggtgac tgttgatgac tattttagca 5160

attgtaattt ttcttttaac tttaaccttt gtgatttggc aaccaaaagg tttagatatt 5220

ggtattacag ctttaattgg agctgttgtt gctatcatta caggagtcgt aagtctttct 5280

gagataaagt ccgtataatt gtgtaaaagt aaaaaggcca tataacagtc cttttacggt 5340

acaatgtttt taacgacaaa aacataccca ggaggacttt tacatgaccc aagtacattt 5400

tacactgaaa agcgaagaga ttcaaagcat tattgaatat tctgtaaagg atgacgtttc 5460

taaaaatatt ttaacaacgg tatttaatca actaatggaa aatcaacgaa cagaatatat 5520

tcaagcaaaa gaatatgaac gaacagaaaa ccgacaaagt caacgaaatg gctattatga 5580

gcgcagcttt acgacacgtg taggcacgct agaattaaaa gtacccagaa cacgtgatgg 5640

ccatttttca cccacagtgt ttgaacgtta tcaacgaaac gaaaaagccc tcatggcttc 5700

aatgttggaa atgtatgtat caggcgtttc aactcgtaaa gtatcaaaaa ttgtggaaga 5760

actttgtggt aaatccgtct ctaagtcctt cgtttctagc ttaacagaac agctagaacc 5820

tatggttaac gagtggcaga atcgtttatt atcagaaaaa aattatcctt acttaatgac 5880

cgatgtactc tatataaaag tacgagaaga aaatcgagta ctctcaaaaa gctgtcatat 5940

agcgattgga ataaccaaag atggcgaccg tgaaattatc ggcttcatga ttcaaagtgg 6000

cgaaagcgaa gagacctgga caacattttt tgaataccta aaagaacgcg gtttacaagg 6060

tacggaactc gttatttctg atgcgcacaa aggattagtc tctgccatta gaaaatcctt 6120

caccaacgta agttggcaaa gatgccaagt tcacttccta agaaatatct ttaccaccat 6180

tcctaaaaaa aattcaaaat ctttcagaga agctgttaaa ggaattttta agttcacaga 6240

tattaactta gcgcgtgagg ctaaaaatcg attgattcat gattatatcg atcaaccaaa 6300

atattcaaaa gcttgcgcat cattggatga tggattcgaa gacgcctttc aatataccgt 6360

acaaggaaat tcccacaatc gactaaagag taccaatcta attgaacgac tgaatcaaga 6420

agtacgcaga agagaaaaga ttattcgcat cttccccaat caaacatcag ccaatcgctt 6480

aattggagcc gttcttatgg acctacatga tgaatggatt tattcttcaa gaaaatacat 6540

caattttgat aagtagaaat ggtaaaaaca ttggattcga cagttgcgga tgtacttcag 6600

aaaagattag atgtctaaaa agcttgtagt taaagctttt tagacatcta aatctaggta 6660

ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc 6720

cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg 6780

gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa 6840

gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa 6900

gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt 6960

aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa 7020

gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc 7080

gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg 7140

ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct 7200

ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca 7260

tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt 7320

catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat 7380

tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat 7440

tctcatttta gccatttatt atttccctcc tcgacttaac tatcaaacgc ttcggttaag 7500

cttaaagcac accctttctg cgtcctcgta ttgacgcgac gtaaaatttc aacgagcacg 7560

ccgggatact taccatattc tctgctaatt atcccgacat catcggtaac aataaatgct 7620

ggataactgg ttgctgacgc atccatataa ctcatcaacc ccggcgttcc atcaggtaca 7680

ggtttcaacg tttcaggatc aagcgctcgc gcatataccc acggcggaac atgtttacgc 7740

tgcatttcat cctcaaagaa acaagtgttg agttcaactt gattaaatat atctcggatc 7800

tgactaatat cactgagatt gaaagtatca aataaaagat gattgaaatc atcacgtttc 7860

agagattctt tttcgtaact tttccagccg cctccggtta tgatataaag gcttttatct 7920

ccagaaaatg agattttttt atctttcata taatggcaga gtaaataaat aaagtatggc 7980

gaaccaataa gacaaagatc tttcccttga ttttttattc gttcaagact attcaatgtt 8040

ttaacaaaat ctattcgttc ttctgttacg gtaaatgtcg taggatataa caattccacc 8100

aaactcataa catatttaaa ccaaatatta tgagcattaa atctatctgg tcccaaattg 8160

actaattcta tttgatgatc aaaccaacta ccaacatatt tcatgccata actcacagag 8220

cctaagagtc tctcaatact taatctgtca cgcgccacct gactttttaa accattcgtg 8280

ccgctactgg taaaccaact ttcaatctcg ttttcctgag aagttaataa gcgagtaaac 8340

ttaaaaaccg atgttgggaa tacaggtatg tcatcaattt ccgtaatatt gtcatctact 8400

ttgtgtgcct gacagtagtg acgatattct cgacaatgtt tataatgatt acgaaatgca 8460

tcaagcacaa gtttctttct gattttttcc tgctcgtcgt aagaccacac taatggatcg 8520

ctcgaaaaaa tcaaatcatc aatttctgag cttgctgtaa tttcttgttt atcaacatat 8580

gaagtcatac ctgttttcct cctcgactta agacagagaa attgcttgat tttcaatctc 8640

aattctcatt cggcgttcat tgactgtcgc aatagttaaa tgttcaaatg acggttcagt 8700

aatatcaaca tcaatatcca gatgatcatt atccatcgcg atagcggctt tcgtaaccga 8760

ttgataaaaa ttgcgcagga ccactaaatt ttcactcaag tcatgcgaac ttcctaacaa 8820

agaatatatc ttgcatcgat tactacgaat atttgataac aatgtgataa cttcatcttg 8880

cttgacccaa ttatcgttat ttgcagtaaa agcaataaac ggtatatcaa gatacatcat 8940

gttattaatt gtagaagcta aatcttccca accaaaatca agacaatctc tcgcaaagac 9000

ttcagcaccc aatttatggc cttcaaaatc tagattatcc ggcaattcat taatgggtag 9060

actgagataa tcaaacccta aagctctttc aagagaatat cttaagttaa caacaccgac 9120

tgcggtgatt aaaaacgaag cattgatttc agataggctt gcataagcta tccgcgcaga 9180

taagcttgaa gccaacatac cgaagttatt tatttttcgt gtagttaacc aatcaaccac 9240

tgctaacaag ctctgctttc ctatagacat tgtaaattca tcaattgtcc ctgaactcaa 9300

tccaacgtgg tgaagcgaat catagcggat cacatgaaat ccattccgcg ataaatattc 9360

cgccagacca gcaaaatgat ccatcctgcg ggcaaaacca gacgcaataa taatggcatt 9420

ctttctcttt gggctgtttt cttctggcag cgtttcccaa acatgaattt ttttatttcc 9480

ttcaacacaa ataacgtggt cgatggtttt atattttgat tcattttcca tacttttact 9540

cctcctcgac ttatgggaca aatacaagga acttatcttc ttccaggaat cgagtctgtt 9600

ctatttcaac cgcaacatcc ttagccgtat agttagatgg cctttcatga gaaatatatg 9660

tcactaatcg ttgcaacggt ctcattccgt catgagatcc accaactcga aatatgttat 9720

tcattcctgc ttctacaatc ctttccgcac cttttaatgc taacgcatct cgatatttaa 9780

atgatgactc ccaaggaaaa atagatatgg tttgcgtctt atttttttga acataaggca 9840

atatttgctc aatattatcg acgtgatgaa ggtacacaca tctgccaagt ggttgattaa 9900

attccacacc tgcatttgac tcaataatca tccaacgttg atgaatatcc acctctactt 9960

ttaatccagc aaacaagctt tctttttgaa ctaaagaata ggccgccttt tcatcaaaat 10020

cttttttggc attcggtaat atatgcgcat atagattaag tttttctatc aacgctaact 10080

taaattcctc ataatgattt cccatgtaat atatgttttg ggcagaaaaa caagctcgct 10140

gatcgtaaaa acaaacatca tgagccgcac ctgtcgctgc ggacgtcaaa tcaacaggat 10200

tatcgataat gcaaagactc tttttagaac caaatttaat cacatcagca taagatggcg 10260

catgctctac cgcccaatta atcgcatctg gccctcccca agcgacaata acatccgcat 10320

gtcgcataat ttcttttgcg agtgatgtat caccttggtg gggccaatat ataacagata 10380

aagagcgcgt tatcggatga ttagggtcta catcaataaa acttaacgct aatgcattag 10440

cggtaaaagg atcggttgac gatgttttta taatacactg attcttagtt aaaattgcgc 10500

gtaatataga catgatccca gataatggaa cattacctgc caacagatgt acagatttac 10560

ctttcggaaa agcccgaaca taactttcat cctgaggtag ccattcatcc atgatatggc 10620

gagaaccaag ttcattttct acaacatcat aaaggccgcc tttagaacat aaaatcatag 10680

atatccaatt ggcctctagc ttagccattt cttctgaata tcccatatat ttttttaagt 10740

cacgaatgta tgtcctgcgt cttgagtatt cttcattttt ccatctttgc cctaccgtat 10800

agagaaaatt gacaatgtta tgcaaccgta attcgttatt tccattacaa tcaataatgt 10860

tttttacatg agagtcattc aatattggca ggtaaacact attatcacca aaattaatgg 10920

attgcactaa atcatcactt tcgggaaaga tttcaacctg gccgttaata atgaatgaaa 10980

tttttttagt catatttgcc atcctcctcg acttaggtat attccatgtg gtacttctta 11040

atattatcat caacaatatt gattacattt ttttggctca tcaaatcatt cattggttca 11100

aaggacagca atacactttt cgcaccacac ttttcaattg ccaacttagc cgcagttata 11160

cactccgtat aatttccgac agcgttttct gcaattattt cttcaagttt attttcgaaa 11220

ttttcattag ggtgcatttc aagaacataa tcactaataa atgcacgcgt ctcttgttta 11280

gctttattac tatcttcgtt atagttaact aatatcatta actgatggtc tatctctgat 11340

aggtcaacgt catatttatc cgcaacggct ttatatcttt cagcatattc atatctaaca 11400

tcattagaat catcccactt aaagatgaga ggaatacctt ttttggccgc ccactcaaca 11460

atatgatgac tggttgctgt tacatatttc cgaggtccgc ctggcgtata agcatgggga 11520

tttacagata ttttagggaa gctataaaaa tcgttatctg gattacaata gcctgttgtt 11580

aaagcatcgt taatgatttc ataacactct tcaaatagtt gctgttgata ttcaaccggg 11640

cgattaaaaa aatgcatttc atcttttttt tcgcaatcac taaaccctaa aataaatctc 11700

ccttcactta actgatccaa taagcaagct tcctccgcta tggcgacagg atgatgagtt 11760

gtaatgatgt gatttaatga accaatttta attttctctg ttaaaccgag cagaaaacca 11820

gaaacagtca gaggagcgcc gacaacacca ttatctgaaa aatgattttc atacactaaa 11880

atctgttcaa aattcaactt atcaacatac tccgttattt cctgcatgcg aactatactt 11940

tgttcttgaa cagttgttga attgatgaag ttaaggaaga acaatccaaa tttcatttct 12000

ttctcctcct cgacttaata taatagcgaa cgttgttttt ctttaagaaa tggcatgaca 12060

tcagactgga agagcttcat ggaagcaata atttcgtcta ctgttccatt agcttcaaat 12120

ccacaacaaa tatttgatat tcctgtagca tcaatgtctt tttgaattat gtcaatacat 12180

tcctgcggcg ttcccacggg attgatttcg taactgtaat caatacggcg attagtatct 12240

ttatgtcctt ttaatacaaa gtcacgccac tgccctttat tgaaatcata acctcttgtt 12300

tggtctgaat catcaaaaat agtcgtagca ttcacataag aatcatacca atgccccaga 12360

aatttccggc aaatctcttt cgctttaatt gagtcatgat ctacagatgt tatatatgat 12420

aagcaatggt cgatattatg aatatcgtgc ccatattctt gagccacttc attataaagc 12480

tcaagttgtg ctttcttttc gttagtattt ataatccaac ttaatatcat cggtaggcca 12540

aattgagcag cccactcagt cgtcgaagct gattcagcca ccacataaac cggtgcgcca 12600

cctctgctat acgccgcggg gtttactttt accttatgga acttgatatg ttcattatca 12660

gcttccatat atccctctgt catgccattc tttatcagcc cgtaccagca ttccgctaag 12720

gcgcgactgt tattcatatc tgtgccgaat acgcgaaagt ccttgttgta aagccctcgg 12780

caaataccaa accgaaatcg tccttttgac atttgatcca ataaattcac atcttcaagt 12840

tggcgtactg gatgggctgt gggaagaaca atagcggcag ttcctacatt caatttttta 12900

gtcgcgccaa gtaaatatgc agcagcgaca taagggttac caagcaaacc aaactccgtg 12960

aaatgatgct ccagtaacca tacggtatca aaaccacact cctcagagat gcgacctaat 13020

ttaaccaaac gtttcattac ctctgtttga gaaaattggg gaggttggta tgtaagcaaa 13080

aagtttccaa atttcataga gagtcctcct cttgcttcat ctgcaggcat gcaagcttga 13140

gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt 13200

gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 13260

cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 13320

tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 13380

gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 13440

ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 13500

caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 13560

aaaaggccgc gttgctggcg tttttcgata ggctccgccc ccctgacgag catcacaaaa 13620

atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 13680

cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 13740

ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 13800

gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 13860

accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 13920

cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 13980

cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 14040

gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 14100

aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 14160

aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 14220

actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 14280

taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 14340

gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 14400

tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 14460

ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 14520

accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 14580

agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 14640

acgttgttgg cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 14700

tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 14760

cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 14820

tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 14880

ctgtgactgg tgagtactca accaagtcat tctgagaata ccgcgcccgg cgaccgagtt 14940

gctcttgccc ggcgtcaata cgggataata gtgtatgaca tagcagaact ttaaaagtgc 15000

tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 15060

ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 15120

gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 15180

cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 15240

gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 15300

ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 15360

cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg 15420

acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 15480

atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct 15540

ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa 15600

taccgcacag atgcgtaagg agaaaatacc gcatcaggcg aaattgtaaa cgttaatatt 15660

ttgttaaaat tcgcgttaaa tatttgttaa atcagctcat tttttaacca ataggccgaa 15720

atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 15780

gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 15840

gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttgcggtcg 15900

aggtgccgta aagctctaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 15960

ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 16020

gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 16080

ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 16140

tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 16200

gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt 16260

aatacgactc actatagggc gaattcgagc tcggtacccg gggatcctct agagtcgacg 16320

gtatcgataa gcttgatatc gaattcctgc agcccggggg atccactagt tctagaatca 16380

gaccatggta cggtgaatat tcaattcgtg cagaaaatct tcctttgtta tcatcaagtt 16440

ggtgccaaaa ttcaccatga aaaaagtatt cttttgttag ttcgtatttt tttgttgcca 16500

tgtactgctc catttgtttc tgagaatcct tcagaatagt aaaaatacta ctcaaaactc 16560

gtcttcaaga tctctggcta tctgctcatt ttcatgatgc tcaacagcat cactgatcaa 16620

attcatcatg ttattaagaa actcctctac aactttaaac gcatcattga gcgtagctgt 16680

attgaatgta tgtatctttt catccgttgt tttcgttttc ccgttacgat gaactatgtc 16740

atgcctcagt ttcatcaatt cattgatatt tttgagtgga aaacgtggat attgcttagg 16800

ttgcaggact gctttatata tttcaactac cagttggatt ctatgataaa gaatatctga 16860

aaggtactcc tgtacatatt tgtttgcatt agactcttta tttatcaact cagacagaga 16920

gatattcttt gctttcagtt catttatgtt tcttatggca ttttctacat atctgttatg 16980

tgacaataca acgcttttaa tcatttcgct aagacagttt tccattattg tcacaatgta 17040

tgcgattttc atttttatga aaacttcact tgtagattgt tttccctctt cttttatttg 17100

ttctaaaagg tttattgcta ttttataaat ttctgtatgt ggatgctgtt ttaaccattt 17160

ttcgttttca tattcttcca tagccatatc agaaagaaag tcctgatact cgtcatagtc 17220

cttttcaagt aactgccatt cttcagaatc ctcctcaaga tctgggctgg aaagccgttc 17280

tcgtatccat tcctcacggc gactttcctc gacttgcata acccaatctt ttgtagctcc 17340

cacaataccc tccttatgtt cattgctaaa tatcaatagt cactttatcg gagactacgt 17400

gaaaaatctt ctcctttccc cctgcttttt tatcagattt cacgcagcgt accgccgcta 17460

ctgtatccat caaaaaaact gatagctttt taatcatctt ttgtgctgca acttactgaa 17520

aaaaggatta atggaaacgc cgataacggc attgatctgt tggccaacgc gaccggcagc 17580

attggactta acaaaaaaac tgaaagaaaa tcccccaaat gaagggaaaa aacggaagaa 17640

cagtcgaacg acaggcacaa aaaaacccga ctggtaatcg ggctttttcg tgttttccag 17700

tcctgagttg gtggctctga ctggaggaag atactctaca tccaacatcc acaatattat 17760

ggcactagac accagttgtc tagtttgact tcagattgtc tgtcaatggc catttcttta 17820

aatgaatttt atgcgtgcat ctgtataatc tttgtgttgg cgctgtacag tgtgcctccc 17880

ttgcttctaa cccctgcgct atctggggat gaacgaatta aagtcctggc tgaacaaaca 17940

ggtggtgctg tgttcagtgc tctggcaggg ggaaggtact ctacatccaa caatgtgaga 18000

gagccgttgt gacaggcgat gtatgagcaa gcaacggtga cttcctccag tatcaaccta 18060

gagaaacgga tgatgaatct gttaatcatc gttctcaggg ctgtgctcgc cattgcaaac 18120

gcgctgattg ccattcagaa actaatcgag tgattgatgg cttaactaag ggagagacgt 18180

ttaccgcttc ctgcccttta acaaaggagt gtttatgagt tttgaacaat tatttacact 18240

aacgacccaa cgtcgttggg tttgccgcag aaaatttatt catttaaaat agatctaaat 18300

gaagcgggaa actgcagacg taaaaaaacc gactggtgag gtcggctttt ttacagttcc 18360

ggtacgagct ggtaactcgc ccggaagaga aactctctac aactgacaac agtatgataa 18420

tccgtgaagc ctgattatgc aatatgacag gcggtgacta cgcgttaacg gtttgcttga 18480

aagttagata aacgtataat cagagggtta gtgctgtggt gagtctttcc ctgcattccg 18540

ctaaatgcgg catgaacagc ttcaaagtcc tgacaggtaa tacaggtggt gctggttatc 18600

tgtgctctgg taggagtgag actctctaca actgacaaga cgaagagaag ccgtggtgac 18660

aggcagcgta agagcaagcc acggtgtctt tactccattt atggagaaaa cggatgatta 18720

acctgttaat catcgttctg agggcggtag ctgcgcttgc aaacgcgctg attgcagttc 18780

tggaactgat ccgtcagttc atcgattgat gactgcggaa acgtaactaa gggcaggaag 18840

tgcaccgctt cctgcccttt aacaaaggag gtgttatgga ttttatgagc attttggggc 18900

gctgtttact tcttcctttc cctgttgcga tgtttgtcgg ttgcctgttt ccaggcatcg 18960

atgatcgttt tactggaatg ttcatgagtt ttattgtcgg ttgtctatcg tggtatatca 19020

ccaagccaaa accgcaaaag gctgaggtcg cgtaagcggc cttttatttt gatacgcatt 19080

agttacaaca ttcaatttgc tttttaaaaa tatccacttc agcacctccc tcataaataa 19140

cgtcaaccag tttattgagc tcaccctacc ctactcaccg gaggattgca gggatttacg 19200

ttttattgcg ctactgattg tgccgcggct gcaccctagt accttctgta cctgtgtcca 19260

ggagctgcca ctttctatca acctgataat tgcatcatgt ctggcttgat taggtttacg 19320

ccctttatat ttgccgtctt tccttgcttt ctctatcccc tgtttctggc gttcccgccg 19380

ttgttcatag tcccttctag caacagcggc cagcatatcc agcagcatat cgttaatggc 19440

tgcaaacaac cggctgtcaa actcactcat accagaatta atccaggtcg tcggcacatt 19500

cacggccata acccggatat ctttctgacg gatcattttc ttcagcgtat tccagtcttc 19560

ccctgacagt cgggaaagtc tgtccacatc ctcgataagc aaaatgtcat tttgctggca 19620

atctctcaga agacggaaga gttccgggcg gtcaagacgg gagccggatt cattctcaat 19680

ataatagctg caaatgatca ggcctctttc cctggcaaaa gcctcgatag tttccagggc 19740

acgcgaagca tcctgttctg ctgttgaagc tctcagataa gcgcggacaa agtttgttgg 19800

tgcatttgaa gtcatattac agaaccagtt cgcataaggt cacaccacta tacccaaaat 19860

gaaccagaaa gtggatcgac aggaaaggca cactctaaaa gaaccgaaaa aacaaataat 19920

gagtaaatgt gacatcgtca cccatactca taaactcaca tgctcatata cacaacaaac 19980

tcaaatgagt atgtgctctt taactcataa actcatatta acctttactc atgcaaagag 20040

tatggtaaac tcaaaaactc acaagagtaa ttgagtagat acacaattga gggtttgagt 20100

atgaaagtaa tctcatttct gaatccgaaa gggggttcag gtaaaacaac tgccgtaatc 20160

aacatagcca ctgcgttgag cagaagcgga tacaacattg ctgtggtaga tacagatcca 20220

caaatgagcc tgacgaactg gagcaaagcg ggcaaggcag catttgacgt atttacagct 20280

gcatctgaaa aagacgtcta tggaatccga aaagatctgg cggactatga ctttgctatt 20340

gtggacgggg caggttcgct ctcagtaatc acctccgcag ccgtcatggt aagcgatctg 20400

gtaattatcc ctgttacacc cagccccctg gatttctccg cagcaggaag cgtcgttact 20460

gttctggaag cacaggctta cagccgcaaa gttgaagccc gctttctgat cacccgtaag 20520

atagaaatgg caaccatgct caatgtgctg aaagaaagta tcaaagacac tggtgttaaa 20580

tctttccgta cggccattac acaacgtcag gtttacgtga aatcaattct ggatggtgac 20640

agcgtgtttg aatccagtga tggcgcagca aaaggtgaaa tagaaatcct tacaaaagag 20700

atagttagca catttgagta attactcatt cactcatata atcaaataag tataacaacc 20760

ggagtaacct taatgtcact tgaaaaagcg catacggcag taaaaaaaat gacctttggt 20820

gaaaacagag atctggaacg agtagtaaca gcaccagtat catctggaaa aatcaaacgt 20880

gttaacgtca attttgacga agaaaaacac acccggttta aggctgcatg tgccaaaaaa 20940

ggtacatcga tcacagatgt ggtgaaccag cttgtagata aatggctcaa agagaacgaa 21000

taatatctga ggatatatca tggataaaaa cgtcgttgat gcgctgaaaa cgctattaga 21060

agcgttaccg gaagaggtgg taacagaagt cacatcaaaa ctaaatcctt cggcaagcca 21120

tattcctgaa gaaaacagta agcaattgac agcaaaagca agactactga atttccggct 21180

aaccgaagcc tatgaagaaa tcctggaagt cgaagctatc agaacaggcc agagcaaaac 21240

taccgttcta aaggcagcac tggcgatgta caacagccag gatgaaaaca taaagaatca 21300

ctggctactt gagtctgcaa aaattaacta attcaggata tagattatta gtaggagttc 21360

gggcaataat ggatgataac tctacgtcag taaccagcaa agaagatgtg caggatattt 21420

ctttaaaaaa ctggctaaag aataaaaaaa ccattgtatt acttatattt actgcattaa 21480

caaatgttgg caatgtcctt tcgacgattg atattatcac agataaaacc agttcttttt 21540

acacatggct tggggaatcg aaaaaattcg aaggccattg gaccaacaat acagaaggat 21600

tcatagacgg cactcctgat tttttactca agaatgcagg cgatgtcctc ataaaatttg 21660

acctgaacat caaaggtggc gaagtgaggg gagagcttca caccgacgca cgactaaaaa 21720

tgtgtgaaac tatagaagaa aagttaaaaa ctttgtgtac attaatagcc tcacatccac 21780

taatgattga gggggaaaaa tcaccatttt ccaatgaatt cgatgcctat attactgagt 21840

atagaaatgg agataaaaaa atagtcgcaa tattaaatat gaaaattaca gacgatgaca 21900

aaatgacaat aacaaataca atgagaacac cagaatcagc attattttca actaaaatat 21960

atgctataaa aataataacc gaagaataac ttaataacaa aaaaggtatt aaagtgaata 22020

ctggaataac aattgatttg accaaccttt cagaagatga attgcttgat ttatattcaa 22080

tgtataaaag tgcaaacata gcacatcaac tatggtgcag acgccatgaa aatataccag 22140

agcatttttc gataatattc gtgacgcttt tagaacgaat aaaaagggtt acagaaaaga 22200

actcagaagg ggtaaaaaca ccagatgtag acctggatgc attaattgac accatttata 22260

ttggttgtcg ttcaatgttc tgtgaaaacc ctggcttaaa aaataactac actctgcaaa 22320

actgcctgag gaaagccaat tatcacaacg aagctagagt gatagataat attttacagg 22380

aaaaaaaatt cacagattcc atcatgaaag atgagtcatt ttttagtctg gtgaaattag 22440

tttccaataa gtccattgca caccaggaga gcctttcagg aaaaaaacgg gaaaagatag 22500

actatcgata taaattctta aatgacaatt caaatatctg tgagtttcag tattacattt 22560

ttagatgtca ccgtatttat gagaatattg tgaaagaata tggggataca ttactgaatg 22620

agcttaaaat aaaaaacaat gatatataaa gaatgccccc atatgaatag aaggaagaat 22680

catactaata aattttccag cttatcgctg gtaagcaatt ctgccagcga atcacatttc 22740

ataaaatcaa tatccatttc gtccagaaca tcacagtagc tgacatcatc aagactaatg 22800

acaatcacag gaacaccctc tttgagatca aacactaaga caggtaatga aatggagttt 22860

atcttttcaa aatcactaaa aaactgttca gttccatatt cactggcagg aaaaagaatc 22920

tcacttaatc tcacacaata aaaaacatga tagtgttctt taaggttatc ctttaaaata 22980

ttaacagcct tagtatattt ttcgttaata aactctttga ccaaatattt cataaaactc 23040

tccaacaaga accgactgta ggtcatcggg caaacgttgc ggaatggcgt cagagacgtc 23100

attttgcggc gtttgcccta tcctgcatcg cagtggcatc atttctggct tatctcgcta 23160

ctgttctcgg tgctttcgtc cgcaggttcg ggaacaagta acttgtgcca gggcatagtt 23220

ttgttgagca gctccatcag aggctcagcc agcagtccca aaatccaggt tgccgtaatc 23280

accggtatag tgagattttg tggcgtccat aactggtgat cgagatgata ccaggtgatg 23340

acacacaaaa taccacccag ccagaacaca gcccatttaa acgcaccaag aatggctaac 23400

acaaaaacaa agataaaatg taaaacactc gcggcaagaa gacgaagata aaaccatccc 23460

cagtcaagcc acttaacaaa agtgctccct gttttcactg caaaacgttt cttacgctcc 23520

ctggcgattt ctttcctgaa tttagcacgt tcttccccct gagggaattg atagatttta 23580

gccatgagtt acctcatttc gatctcaaaa cgaagtttat gcaattatcc tctgaaaccg 23640

tatggtattc tgatatctag gccttcactt tctttatcta ttttataaga aaaccttagc 23700

tttgatgctc ttcttgcgac tctctcaacg attttaactt cgagatgatt aatctctgta 23760

tattgattta ttttttcaat gtttttttgc agaaaaactt tattaaagtc cttaaaattc 23820

tgataaaggt aaactttttc gccattatct attctgaata attcaagttc atcctttaat 23880

acatctactt caatgtcaaa aaatgtcttt ttaccactgc tgtattgttt cctgataaac 23940

tgataaagca tatttgaatt ttgatcagca agtcttacag acgatatcaa aacctgagta 24000

gtgtaagaat cctttaacat cgtgatatat ggctcaatga catgtgaaaa agcaatatca 24060

atatatcctt cactttcaac ataatcacaa aacgcagtca ggttcataag cctgactgca 24120

tcagaaggta atttacctct cttcttcgta tgtccagaga tttgattgcc ctgagctgtg 24180

agaagttgat ttcgaggaat gcgaataaca ctggcctgaa gttcttctgc cccctctttc 24240

aactgccggt aagcacctgt tacatcaata tcagcgataa atgcatattc ccgagccgta 24300

atacgaaagg ttgccccctc agataactct tcacgtgaat caatctgcgc catagccata 24360

aacaaaattc ttctggcaga caaaggcaac gatgagaatg tgctgttaat ttcatttctg 24420

tggcgaattt ttgttttctt tgtaacgctc agtaagttca tacattacct gtggttttta 24480

agttctaatg gtgaaatcat aaccacaaag gcggaaatat gtaaagtggt ttatccacct 24540

tatgtatttc caccaaaaca cggatcacat gaaagaattt ttatcattaa tattcaatat 24600

gatagaaatg cgcaggtatc caccttttaa cgtcatacgt tagattagag cactataaag 24660

aactaaagaa atgaagatac aaagatacga atatctgaat aaaaacagga aaatgcgtac 24720

ccatccacct ttcagtgcgt acccatccac ctttcagtgc gcagccatcc acctttcatg 24780

aaaattacag tgcgtaccca tccacctttc agtgcgtacc catccacctt tcattttgag 24840

gcgacaaaaa acaacaccta tcttaatgaa attattgata aaaaaactgg cacaatgttt 24900

gcagaataag ttaaagtaag ttaaagatct taaagtagat ctaccgatct acatttaaga 24960

tcttaaaaac aagaagttta ataagcaaaa aaacacaaaa aatctgtgga taactttgct 25020

caaaacaaaa aattcacact acctgtggat aactttgcgt aaacccggag gacagatcac 25080

tctgaaccaa gacaaccaca cataaaaaca atatgctcac tttttaacca ctgcgcgtaa 25140

ttgcctgatg ttatccttgt gctgttccgc ttcctcgctc actaactcgc tacgctcggt 25200

cgttcggctg cggcgagcgg tgtctccttt ctcaaaacca gaagagttca gatcatacga 25260

tccgttgatt gatccttttt ggatctttca ctgagaagcc ctgaatcgct ttctgtcgct 25320

tcgttcgata caaccatggt gaaaccatgc gaaatcgaaa aaatcgctca gaatgcgata 25380

cagcgcgttt tagagggtat ttacaaaaca gtcgttgcct tgaggaaatt ttgatcaaga 25440

ttgaactcct atccggatca tggcaacaga tcacagcaac aaggccgcta aaaagcgttg 25500

agcggcgtta aacaccgtca aaccgtagcc gactacaacg aaacgggaaa aacgctcaga 25560

acgcgttaca gagcgtttta aggcgatgtc ggaaagagcc atatctcacc tgttctgatg 25620

tttttctcaa catgagcact tttaagagtg cttttaaaga tgctattatg ccaatgagac 25680

aagcggaggg cttatgagct atcaaattct gacaaccaca gcggccagta ttactgacct 25740

gaaaaaaaat cctatgggaa ccgtagctga aggtgaaggg gacgctgttg cgatcctgaa 25800

ccgaaacgaa ccggcgttct attgcgttcc accaaaactt tacgcctact atcgggaact 25860

cgctgaagat gctgagttaa acgctgttgc tgatgagcgc atgaaaaacc cggaaattgt 25920

gaaggttaac ctggatgacc tatgaactgg cttttgaccg tagagcactg aaggaatggc 25980

agaaactcgg ccacaccatc cgtgaacaat tcaaaaagaa actggcagaa cggctggaaa 26040

atccacgcgt acccgcagcc cggttacatg gtcatgctga tcgctataaa atcaaacttc 26100

gtgcatctgg ctacagactt gtatatcaag tcattgatga gaaagtcgtt ttacttgtta 26160

tttccgttgg aagaagggaa agcagcgaag tctatcagat cgcagatttg cgctaagatt 26220

aatcatcaaa ggattaactg tttactgtca gaacaagagc attaaaaatt tgccacacct 26280

gcaaatgcag gtataataaa acacaagaaa gggaaacagg ttctctttca tccagagccc 26340

aaagtgggcg ggaggttaaa aatgcaaaac tacgctaagt ctgtagccac agagatttta 26400

cgtcaacttg gtggtaatcg ttttattgtt atgactggtg ctaaaagttt ttcttacttt 26460

gatgaaaacg gtgagtgcgg gttaactttc cgtttgccgt ccaattttgc aatgaaaggc 26520

atcaacttag taaaaattaa actggatttt actgatacgt accaggtgaa attttctcgt 26580

gtacggggtg atgaagttaa agatatttca agattcgata atatctattg tgatcagtta 26640

gcgtgtttat ttacacaaga aacagggtta cataccgtgt tatagataaa aaggggcatt 26700

aagccccttt tttagttatt atgaggaata atatggataa agataagata attaagaaaa 26760

acagaggtaa ttactcatat gtaatcagaa cgatggatga agatggggat gcggtttttc 26820

acgtcttaaa atatgttaag acgattgata aaactaaaag caggaaaaca gtaagaaaat 26880

tgataatgga cgaaaaactc aacctggcat cattgatgct tctggataat gggattttgt 26940

gtgattgtct gacaaaaggg gatgaaaatg cagaataaac ctacacctga agaagtaaag 27000

aatgcacggg ttgcggcagg tcttactctt aaagaagctg ctgatatttt tggttatcaa 27060

ctgaattcct ggcagatgaa agaaagtgca ggtaaggcca gtcgttcttt atctgttggt 27120

gaatatcagt atttattgct attagcaaat atgcatccgt cttacaggct ggtaaaaaaa 27180

taacttgcta tacctgcaaa tgcaggtata ataaaacaca agaaagggaa acaggttctc 27240

tttcatccag agcccaagct gggcgggagc taaaaatgaa agtattaact tttaaaaatg 27300

atactgtctc tgttggtgat gtctttgtat catcctgggg ctatgagcaa accaatgtaa 27360

ccttctacca ggttctttct gttcacggta aaaaaaccgt caccgttcgc gagattcgcg 27420

ctaattcaga atataccgat tcaatggtcg gctttaaaac tcccgtttta aatgatttta 27480

ctggtgaatg ttttaagcgc cagataaaag attttggtga tgagctggca atcaaaatcg 27540

aagattttga aactgcgtat aaaactctac cggaagaaaa acatcgattt tcttcttact 27600

actgattaat cagggggata ttcccccttt tagtaaaggt ctgaaaatgg aaagagagtt 27660

tagcgcaaaa gcatcattaa accgaaatat aaaattttgg tttgagcaat gtgggttatc 27720

taaagaaaga gttattcgtt gtattgataa ctggtatgac cttgcatacc caccatcaga 27780

acaggagaaa gcaaaaaaag aagcaattga gaagttaata aagtaaagat aaagatggta 27840

tcaggttgtg gggcaacctg ataccgtgac acctgattct attgcgagga ataaaatgtc 27900

ggaacgcata ttatcagcaa taaatgacgt tgaaaagggt gggcgtccgg ttttcccttt 27960

gatgccattc catgtctttc ctgagtatat ggcattactc agaaaagcac tggaaaaaaa 28020

gacacaaaag agaacagata aataaaccgg aggctttatg acaaaacatg attttgtgtc 28080

ctttgtcagt ggagaattgc ggcaaggcgc tgtacgcttt tctcttgctt ttaacagcaa 28140

aggagaaatt gttctgcact ggactaataa agcaggaata cgagtatggc ggatattaag 28200

tggtaatcgt ggcaaaaaac caagcaaagc taaccttgaa agaatgagta acttccggcg 28260

ctggcttttt gatgcccgtc agggcatgga aggctacact cagcaacctg aacagagcaa 28320

ccttagctga atagccgaag ccctcccgta tttcgggagg tgctttctga tatattgcgc 28380

gggactgcgc ttgcgcttcc tgccagaccg ttcgcggagc gaacctgacg gcactgtcct 28440

taccgcattg atcgggtttc cggtcgcaga cgtacgtatg ttcagccatg acaacatcag 28500

cctttttgca tttttgttga tgctcaagca caatttatga gcggtgccag attcactgcg 28560

atgcaggata gggcaaacgc cgcaaaatga cgtctctgac gccattccgc aacgtttgcc 28620

cggtgaccta cagtcggtta ttgtcggaga attttatgaa aaaagttcaa ttcagaattg 28680

atgaaaatca gcataatgat ttgctggatt gtcttaaaac tctttatcca gatgaaccag 28740

ctttaacagt agctaaaggc atgaaacttt tagcaaatgc tttattaaaa agtaaagctg 28800

gcagtaagga cataaatacg ttttttgata ataatgattt tatcaaaaca acgatgtact 28860

taacaggtaa acaaagggct gatattgaaa gagctgctaa tcgtcacgga tggacgttat 28920

cacgagaatg tcgttaccgc atacagacga cacttgaaaa tgaactggat ttctttgacc 28980

aggaactgct gatgatgaat cgttgccgta attcaattga taagatcggt cgtaatttcc 29040

attatatcat tgttaatgat cagaccaggg ttcttgataa agatggtttc tatcaggatg 29100

cggagcgtct tacaacagaa atttttaatc ttaagaatca gtttgagaat tacattatgt 29160

tatgtaaagg gagaactgtt tcaaataaag tggagatgta attatgggcg tttacgttga 29220

taaagaatat cgtgttaaac gaaagtcatc agaaaatggt cgtaagtcag ctttcgctca 29280

caaagtcaaa aatggtggaa agaactatag ccgcaatgtt caggaacgta tcaaccgcaa 29340

gggtgccagt aaggaggttg ttgtcaaaat atctggaggt gctgttactc gtcaggggat 29400

tcggaacagt attgattata tgagccgtga gtcagagcta ccagtgatga gtgaaagcgg 29460

tcgggtatgg atgggtgccg aaattctgga ggctaaagag cacatgatag atcgtgctaa 29520

tgatcctcag catgtgatga atgataaagg taaagaaaat aaaaaaatca cacagaatat 29580

tgtcttctcg cctccagttt tagcgaaagt aaagcctgaa gatttgttgg agtctgtcag 29640

gaaaacgatg cagaaaaaat atcctaatca ccgttttgtt cttggatacc actgtgacaa 29700

gaaagaacat cctcacgttc atgttgtttt tcgtatccga gataatgacg gtaaacgcgc 29760

tgatatcagg aaaaaagatt tacgggaaat tcgtacaggt ttttgtgaag agttgaagtt 29820

aaaaggttat gacgttaaag cgacccataa gcaacagcat ggacttaatc agtctgttaa 29880

agatgcacat aatacagcac caaaaagaca gaaaggtgtt tatgaggttg ttgatattgg 29940

ctatgaccat tatcagaacg ataaaacaaa gtctaagcaa cattttataa agctaaagac 30000

tcttaacaag ggggttgaga aaacatactg gggggctgat tttggggact tatgttcgcg 30060

ggaaagtgtt aaagcaggtg atcttgtcag gctgaagaaa cttggtcaga aagaagtaaa 30120

aatcccggcg ctcgataaaa acggtgttca gcatggctgg aaaacggttc acagaaatga 30180

gtggcagtta gaaaatctgg gggttaaggg cgtagacaga acaccttcag ccagcaaaga 30240

gctggtactt aacagccctg atatgctgct gaagcaacaa cagcgaatgg cgcagtttac 30300

gcagcagaaa gcatccacgt tacagtcaga acagaagctg aaaacaggga ttaagttttt 30360

gggcttataa gatttaatgt attgattatg cgagaaaaat atttatttta gttcgcttgc 30420

tcatcttttc accttaaaaa cagatcgaaa ttctgcgttt tagctatcat tcgatctatc 30480

gatctaaaaa acagatctgc aaaagatgaa tattctgtgt ttcattgcat acatggaata 30540

aatgaaagta aaattgattt atcaagatcg aaagatctgt tttatgtgtt gaacttagca 30600

taagccactt gtagtataaa cttcgcgcaa tagtaaaaag tctctcttca taggggcgtg 30660

atttctcaca agcaaccacg gttggcttgt gttttgtcac atctgtatat cggggattga 30720

gtatgaaagg acagaaacag ttttacctgc ttcagtgttc tccgcatttt catcagtcgg 30780

ttctgttgca gttatcccgg agcggtatcg cttactacaa tccagttata cgcactttct 30840

ataagaggcg tgactgtagt gcatacaggg gacaaatcat gccaatgttt cccggttatg 30900

tattcgttct tctggatttt gaggttgtac atccatcctg ctttacccgg atgaaaaatg 30960

tttatggact ggtcagtttt ggtgaatatc ctgctgaagt tcctctgagt gtcataaatg 31020

aagtaaaaga gcaggagaag attttttcga tgaatttaag cctgatgaat aactcctgtc 31080

tggcaaaaat acttctcctt gcagacgctt caaagcgtgg acgagtcttt gccgactatg 31140

tgtgtaacag aaaatacgac aggttaaaaa atgacagcta taaaaggaaa gcggaaaccg 31200

caacgcaatg tactctacct tcctacagaa gttcgtgttg aagttgaaaa aattgcaatt 31260

gaaataagct tcaagagggg gcgacgtatc tctgattctg gttttgttca gtatcttatt 31320

aaaaaataca aatctcaggc gatgaaagaa cttattcatg gggctgatat tcctgacgag 31380

taatattgtc ccaatgttct gaatgtggaa tatttatgtt atctacttct acttttcttg 31440

cgcttgccat gcaatgcgct gccagcgttc atcccgacac aacgcacgaa gtcgccaggg 31500

ttgaatcagg ttttaaccca tatgcgattg cggaaataat accaaaggtt aaacgtaaaa 31560

ctggtgataa aggcgtagta tcttactttc ctgaatcaaa ggaggcagca cttaagatcg 31620

ttaaaaacat tgaattacgg aatcatcgtt actctgtagg acttatgcaa ataacgagta 31680

ccaattttgc aaagttcggt acaacagcag agaaaatgtt tgatccatgc gaaaatctta 31740

aggtatcaga aaaaatactg gttgactgtt ataaacgagg tggcgactta gtgcgtgggc 31800

tgagttgcta ttattctggc aatcaagaaa caggagtaaa gccagaacct gaatttaata 31860

atacaagtta tgtacaacgt ataggattta gccctcctga taataaaaaa agttttattg 31920

ttccctctgt aaaggaaatg attaaaaagg agaataagac gactatcaca cctgaagaaa 31980

tcattatata tcctcaatac gccatgcgtg gcactgtatc aaatgaaaag gaaacaaaag 32040

atgttgaaat taaatctgaa taaacgttat ttaacgcttt ctctatttat ggctgcgttg 32100

atgctttgtg ttgcagaacc tgcttttgcg gatgatgtgt ctacgaagac aactggtttt 32160

ttacagaaaa taattgattt tttaacggat attcgtaaac ctgcaattac aattattgct 32220

cttgttattg ggtatattgc gatattttct cgtcaacata cctcatggat agtcccactt 32280

gttatcggaa ttatcatctt tattgttgca ccatatattc ctgactggct tgcgtaatta 32340

aaggatgggc gcactatgag tactcttttt aaaggtctta cgcgccctgc tttaataagg 32400

gggctgggcg ttccgctcta cccctttctt ggaatgtgca ttatttgtgt tctgcttggt 32460

gtctggattc atgaggctat gtatgccctt attcttcctg gctggtatgc catcaggcgt 32520

gtaacacagt ttgatgaacg cttttttgac cttctgtatc tgagaactct tgtcaaaggg 32580

catcctttat caaacaagcg attcagcgca gtccattatg cggggagcca gtacaatgaa 32640

gttgatattt caaaagtgga taactttatg aagctgaaag accagtcttc tgttgaagag 32700

ttaattccgt actcttcaca tatcactgat aatattatcg ttacaaaaaa ccgggatttg 32760

ctggcaacct ggcagataga cggtgcttac tttgagtgtg ttgattctga agatttgtca 32820

attctgacag atcagcttaa tacgcttata cgtagctttg aagggaaatt tgttacgctt 32880

tatcctcatc gtatcaggtg taaaaagggc gtcagaccag tatttaacag taaaattcct 32940

tttgtgaaca gagtaatgaa tgattattac gagtcattcc ctcagtctga atttttcgag 33000

aataaattat ttctgacgat ttgttttaaa ccttttacta cggaagataa agtaacacat 33060

ttcttttcac gcagtaaaaa acaaaaagat atctttaaag agcctgttaa tgaaatgaat 33120

gaaatttgcg acaggttgaa tacctatctg tcccgttttc attcccgacg tcttgggctt 33180

tatgaagatc atggggttgt ttattcagat cagttatctc tgttccagta tctgctttct 33240

ggtcgatggc aaaaggtcag ggttagcagt agtccgtttt atacatatct gggaggaaaa 33300

gacctgttct ttggtaatga tgccggacaa attaccgcgt cagaccatgc ccggtatttt 33360

cgttgcatag agattaagga ttattttcag gagacggatg ccggtattct ggatgctctg 33420

atgtatctcc ccgttgagta tgtcgtgaca tcgtccttta ctgcgatgga taagcagtca 33480

gcgattaagg cgctggatga tcagatcgat aagctggaaa tgacagatga tgctgccaaa 33540

tctttgctgg cagatctgaa agtcggactg gatatggttt ccagtggata tatttctttc 33600

ggaaaatcgc atcagaccct ggttgtcttt gcggattcac cggagcggct ggtgaaagac 33660

accaatatcg tgacttccac tctggaagat ttggggctga ttgtcactta ttcaacactg 33720

agtcttggcg cagcttattt tgctcagcta ccaggaaatt atacgcttcg ccctcgtctg 33780

agtaccctca gtagtcttaa ttttgccgaa atggaaagtt ttcataattt cttttcagga 33840

aaagaaaaag gaaatacctg gggggaaaaa ctgattactc ttcgggggtc aggtaatgat 33900

atctaccatc tgaattacca tatgacgact gaacatcaga atttcttcgg taagaacccg 33960

acgctggggc ataccgaaat tctcggtacg tctaacgtgg gtaaaaccgt attactgatg 34020

acaaaagcat ttgccgccca gcagttcggt acgccggaat cattccctgc aaacagaaaa 34080

ctgaaaaaac tgaccacggt tttttttgat aaagaccggg caggtgaagt cggtatacgg 34140

gcaatggggg gatcttatta ccgggtgaag gagggagagc cgacaggctg gaatcccgcc 34200

gcactgccgc caacaaagcg taatatcgct tttatgaagg acctggtgag gctgctttgt 34260

actctcaaca gtgagccgct cgatgattac cagaacagcc tgatttcaga tgcggttgaa 34320

cgtcttatgc aacggtcaga tcgctcttat cctgtcagta aactacggcc tcttatccag 34380

gagccggatg atactgaaac caaacgtcat ggacttaaag cccgtcttaa gccgtggacg 34440

cagggggaag agtttggctg ggtgttcgac aatcgggaag acacgtttga tgtcgataac 34500

ctggatgttt tcggtattga tggaacggag ttcctggata ataaggtgct ggccagtgct 34560

gcttcattct atctcatcta tcgggtcacc atgctggccg atggtcgcag gcttcttatc 34620

tacatggatg agttctggca atggatcaat aacgaagcgt tcagggactt tgtttacaac 34680

aagctgaaaa ccggacgtaa actcgatatg gtgcttgtcg tagccacaca gtcaccggat 34740

gaactgatta aatcacccat tgcggcagcg gttcgtgagc aatgcgccac tcatatctat 34800

ctggcaaacc cgaaagccaa acgtagtgaa tatgttgatg gtttgcaggt cagggagctt 34860

tattttgaca aaattaaagc tatcgatccg ctgtcccgcc agttccttgt tgttaagaac 34920

ccacagagga aaggtgaaag tgatgatttt gctgcttttg ccagactgga gctgggaaaa 34980

gcagcgtatt acttaccggt tctcagtgca tcaaaacccc agttagaact gttcgatgaa 35040

atctggaaag aaggaatgaa gccggaagag tggcttgata cctatctgga acaggcgaac 35100

ctgatttgag gaatcaccaa tgaaaaagca aattatggcg gcattcgtcg cttcactgat 35160

tgttatttcc ggcgctcagg cagggatacc tgttgccatc gacgccaacc ctgaatgggc 35220

gattgaagcc ggacgatgga cagaacgcct taagcaatgg gcggaaacgg taaaacatta 35280

cgaaaatcag ataaatgcgt acaaacagga gctgctgtca aaaacgggta tccgtgatgt 35340

gcagggactg gtgcagtccg cacagtcagt gagtcaggaa ctgatgcaga tttatgatca 35400

ggggaatgct tttattgacg attacattaa aaatcctgaa ggggcgttat cggaacaggc 35460

caaatcgtta ttgtcagatt acaaagtaac ggatacctgc cagaacctgg gatattccgg 35520

cgacctggta cggggatgtg aagcgacgtt cctttctcaa ctggcaagcg tggaatacgg 35580

taacaagctg gagagcaagc ttcgtcagga caaccagacg atgaaagacc ttattgatca 35640

agtcaaaaat gcgaaggata cgaaggccac gcaggatgca acaaacgctg ttgcacttga 35700

acaactgaag ttcgagaagc tcaaatttca gtatcaaatg tatcgcgata agcagcgaga 35760

tcttgcagaa tataaagaga agatggctca ggcagctttc agaaaacagc aacgtgaagc 35820

cgtgccacct tcttacagaa aagcttatat ggcaatgaaa tcatatgagg atgattaaat 35880

gaaaagtatc gctactgctg gccttgtcat tatatctgtt tttagtcttt ctggatgttt 35940

tgaagaaaca aaatcggttg attggtggct tgcacatccg aaggagacat acaaaaaatt 36000

tgaagagtgt cagaagtctg gtagcgattc tgataattgt aagaacgtta agcgagcaca 36060

tttgtctttt gaacggagaa aggcggttgg tttaccaata aattgagggt gatgtatggg 36120

gattgtcact ggtataccgg atggtactga tttatcaggt aatgtaacct ctcccgcaaa 36180

tgcgggaggt gtttctggat ttgattctga tttttttcag acaactcatg aagttatatt 36240

taatattctc aacaagagta tatctggaaa attaagtgaa tattcagatg tggcttatac 36300

tcttggtaaa tatggagttt ctctgtatgt tttatggtat gcttttactg tattagcaag 36360

gaaacaacag acacctgtac ctgattttat ctggaatatc tgtaggtttt acataatatt 36420

gctttttgtt aagaatacag ggggaatact tacatcagca acagatgcta ttgatggatt 36480

gaaaaataca ttggcagggg gagatccgtg ggtatggatg gatcagttat gggtgaaggt 36540

tatacaagtt gcaactctta tttttgataa agatacatct actgtgcctg ttgccggtgg 36600

gattggtgct ttattaactt atgtcggtgg tgttttggca ttattgcttt gttctatagt 36660

atttgcatct gctgaattaa cattactatt actttctgtc actgcgccaa tatttatcat 36720

gtgtctgatg tttggtttac ttcggcaaat gtttaatagc tggctacagc ttaattttag 36780

ctcgttactg gtttttctat ttgcagcatt agcactaaga gctgggacat ggcaattaaa 36840

catggcatta agtacgtcta ttgctacagc atcagaaaac aatcttcttc aaacgggagt 36900

aacttcatta gctgctggca ttttcatggc ctggattatc tggcaggcga aaagttatgc 36960

ttcacagatt gcaggtgtgg gtgttgaagg tgccatgcag ggcgcagccg ctatggggat 37020

tggtgctggc gttttcggtg catcccgtat ggcgcgtggc gcacttggta tgggcagaaa 37080

tgccggtatt ggtgcatgga aaggtctgcg tcgtcaggaa gacgggtttg gtcagtctcc 37140

gggaataacg ggtaagaccg ctaacctggc cggacagggc gttaatattg gtgccaaaaa 37200

gcttcgtcag gcagctattg agagagcaaa gaaaatgtat ggtgggtaat ttaattatga 37260

gcgcctttta atgttctttt cataattgtc attgtcgggc ctggcgcgct ctattatctt 37320

ttctgttcac actgagtctt ctgtaaatat taatctgtta tacaggtgtt catattatga 37380

aactacttat tgtggctttc gtgacgcttt gtctcgctgg ttgtcaggcg tcacataaac 37440

taccgcccgt ttccgggaaa agcgaacctg ttaattctgc tgaggtaatg caaaatggaa 37500

tttaaacttc ccggatttaa aaataaaaaa gacgttactg actcatcagt ttcatttgaa 37560

gaaaaaaaca ttgcactaca ggagagaatg aatcgtattt ataaattcgg tggtatcgga 37620

ggcatgttaa ttggtgggct gtctttactt gcattaaatg cagcattacc actgaaaaca 37680

acagttgttg atgcctacct tatcgataag gttacaggtg tggctgaacg tctgacttct 37740

gttaaaaaag aaaatctttc tgaaaacgaa gccattgccc gatattttat cacccagtat 37800

ataaaacatc gtgaaggtta taattttttc agtctccagc atgattatga ttatgtaatg 37860

gcttacagcg cggagaatgt cgcggcagat tataacgcat tatttaacag tgaacaggca 37920

ccaaaacttg tttataacaa agcagaaaaa acggcaatgg ttcaggataa tccatctgtc 37980

ataatttcac cttcgtcacg ggcagatgat aaagatatcg gtgcgtatat tcgttttcgt 38040

ctgaccatca gggatgttgc taccggacaa acccgccagg agttctggaa tgttcgcctg 38100

acttatcgta tcgaaccgca ggttgaaatg gtgtcagggg aacgtaataa caatcctctt 38160

aaattcgttg taacaagcta cgttcgcgat aaagaagcca gaggttaata atatgaaaat 38220

gaataaagga gcgttaatta tggcgcttct gatggcggcg cacgtctgtc atgcagctgt 38280

tcttccttca ggcagtcgct ttgacccacg caatcagata gtcagttata accccaataa 38340

taccaccata attaacagtg ccgttggata caccaccaca ctggtatttg atgaagatga 38400

aacagttatc agtgccagaa ctggttttcc gcagggatgg gcggttaata aagaagataa 38460

cctggtatac ctggaagttc gtcctgttaa acagactgtt cagaaaaata atatggatga 38520

aaacggtaat acctcttctg aatccgtcag tgttgctctt gacccggaaa atgagcttga 38580

acgctggcga acgaatttgt ttgttcgcac cacgaagcgt aattacagca tggagctgaa 38640

cgcccggacg ttccggcagc cggagaaaat tgcgtttgtg gtgaattacc agtatccgca 38700

ggaacgccgg aaggaacagg ccgaaattga gaagaaacgc acagaggctc ttgccagacg 38760

ccaggaggag caggcaatca accgttccct ggaaaatgcg aaatcgcccc gtaactggca 38820

gtactggaag cgggttgctg aaggcagcca ggatatcagc cctgattatg catatgacga 38880

tggccgttat acctggttcg gcttcagtcc gttaaagaaa attcccagcg tctttgtgat 38940

gaacggtatg caggagactc ttaccaatcc tgtgattaaa cagagcggga gttttacggc 39000

tgttggcgta ccggttgata agcgttttgt tttacgtctt ggtgagcagg tggtggggat 39060

tgagaaccag ggcttcggaa aagtacgttt accagccgga gatacggtat ccccggatgt 39120

taagaaagag gtgatccagt gactgaacag gaaaataaaa tcccgactgc aaccgaaatt 39180

gaacagcagc tacgggaacg cagacagaaa gaactggaac aggccgggaa gactccggaa 39240

gaagagcctg gcaagccagc attgcagctt ggtattgaaa aacttaaaaa gtcacgtaaa 39300

gggatgatta tcctcgtcgt gggttttctt ctgcttgctg ccggtgtttc tgtttattat 39360

atcccgtcca ttatccgttc tgtgtcgtca ggggatgaga aacccgcaag tcagccgctt 39420

gcaaccggaa cggctaaacg tcagaccgga ctgagcgaag atatcgatcc ttttaatacc 39480

gcacagaaaa aaacagagaa accagaggaa gaaaaagtca tttcttctga aaagactgaa 39540

ccgccggaaa ataaacagca gagcttcagc cgtgcacttg acgtttctct tgatggcagc 39600

cagacaggaa acagcagcag ttcagcggga acgtcagttt cacatactgc ggccagtgag 39660

ccagaaagcg ataaaaagga tgaagcaaaa gcaaccgcac agactacaga atctgcgcca 39720

ctggcgaaaa taacgaaact tccatatgac ccaaatttgt ttatcccgga agggacatca 39780

attccctgtt cactggacag gcgttttgtt tctgacctgg cggggaaact ggaatgtacg 39840

gtcaacagcg atatatacag cgccagcggt aatgtaaaac ttatcgaaag aggaaccgcc 39900

gcaaaactga tgtataaggc cgggtcttta aatcatggac aagggcgtgt gtttgttatg 39960

gcttacaagc tacgtacccg cagtaagcct tttattgata ttcccctggt tgactcacag 40020

gcggctggcg cgttaggcga agccggtgct tctgggtgga ttgacactca tttcagtgaa 40080

cgttttcttg gtgcaatgat ggtcgggatg ataccggatt taagtcaggc cgccagtggt 40140

attgcacaga acaacaggga cagccagacc gactatacgg caaacagtcg ccaggctttt 40200

gctgatatag cacgcgaagc attttctaat agtgtgaata ttccgccaac gctttataaa 40260

aatcagggcg aaattattac tctgattgtc ggtcaggatc tggatttttc aggcatttat 40320

aaactgaaaa tgaaaggggg ttaatgtgaa taacgaaaac agacatctga tttatgatgt 40380

ggtcaacgat tatttttatc actggctgaa tgagattgag ggtgtcacgg aaattgctgt 40440

taaccgacca ggagaaatat ttataaaggt caggggaaag tggcaatggt atgaacaaaa 40500

gatgagttac agtgattgtc tttcttttgc atccacactg gccgattttc atgacggcgg 40560

ttctgtgact cctgaatatc ccctgcgctc tgccacgctt ccgggtggag aacgtgttca 40620

ggttgtgatc ccaccggcaa ctgaaaaaga cactgtttct ataacaatcc gtaagccgtc 40680

aggtattttt atcagtcatg acaaatttat aaaacaggga ttttattcac gcgtcagtgg 40740

tttaagtggt gactcggtta tggaagataa tatttctgct ttaatcactt ccggatattt 40800

tgatcgggtt atacctgaat cactgcgtca ggggaaaacg atagttttct gtggagggac 40860

gggttcaggt aaaactacct ttgcaaatgc ctgtctggaa tatataccgc atcatctgcg 40920

gtgtatttct attgaagata ctgatgaggc aaaattcaga ttccataaaa accatgtaaa 40980

actttactat ccggcagagg gtgagagtaa ggttattacc tcagcgagtc ttctgcgttc 41040

ctgttttcgt atgaatccgg acaggattct gatgacagaa atcagggggg ctgaggcatg 41100

ggattttctg aaagcatcga gttcaggcca tgcaggaaac attaccaccg ttcacgaaag 41160

tagtcctgaa tatgctgtgc ttgggattgt tcagcgatgt tatatgaatc ctgaatgtca 41220

gaatctacca ttcaatgtca ttttaagacg tgtactgagt aatattgata ttatcatgag 41280

tattaaatac cttgatgatg aagattttcg tttcgcttcc ggtatttatt acaaacaact 41340

tcattttgat gactatttca gaaaactgaa ggagtgatta tgtctttaaa actccccgat 41400

aaaggccagt gggtttttat cggtctggtt atgtgtctcg tgacatatta tactggttct 41460

gttgctgttt acttcctgaa cggaaaaacg ccgctttata tatggaaaaa ttttgattcc 41520

atgctcctgt ggcgaataat aacagagagt aatatacgga cagatatcag gttaaccgct 41580

atcccctctc ttttatcagg tatggtttcg tctctcatcg tgcctgtttt tattatctgg 41640

caactgaata aaacggctgt tgctctttat ggtgacgcga agtttgccag tgataatgat 41700

ttaaggaaat cgaaacttct gaaatgggag aaagaaaacg atactgatat tctcgtcgga 41760

gcatataaag gtaaatacct gtggtatacc gcaccagatt ttgtatcact tggcgcagga 41820

acccgcgcag gtaaaggtgc cgccattggt atccctaatc ttctggtcag aaaacactct 41880

ctgattgcgt tagatccaaa acaggaattg tggaaaatca ccagtaaggt gcgtgaaata 41940

ctgctgggta ataaagttta tctgctcgac cctttcaaca gtaaaacaca ccagtttaat 42000

ccccttttct atattgattt aaaagcggag agtggggcta aggatctgct taaactgatt 42060

gaaattctgt ttccgtctta tggcatgaca ggggcagaag cgcactttaa taatcttgcg 42120

ggtcaatact ggacaggact ggctaagttg cttcatttct ttattaacta tgagccgtcc 42180

tggcttaatg agttcgggct taaacccgtt ttctcaatcg gttctgtcgt cgacttgtac 42240

agcaatattg accgggaact gatactcagt aagcgggaag aactggaggg aacaaacggg 42300

cttgatgaaa acgcgttgta tcatttgcgc gatgccctga ccaaaatcag ggaatatcac 42360

gaaacggaag atgaacagcg ttcaagcatt gatggttctt tccgtaagaa aatgagcctg 42420

ttttatctcc caaccgttcg taaatgtact gatggtaatg atttcgatct ccgtcagttg 42480

cgacgggaag atatcactgt ttatgtcggt gttaatgcgg aagatatatc actggcttac 42540

gattttctga acctgttttt caacttcgtt gttgaagtga cattgcgtga aaatcctgat 42600

tttgatccca ccctgaaaca tgactgcctg atgtttcttg atgagttccc ttcgattggt 42660

tatatgccaa ttattaaaaa gggatcaggg tatattgcag gttttaaact taaactgctg 42720

acaatttatc agaatatcag tcagctaaat gaaatctatg gtattgaggg agccaaaacg 42780

ctgatgagtg ctcatccctg ccgtattatc tatgctgtca gcgaagagga tgatgccgcg 42840

aagatatcag aaaaacttgg gtatattacc actacatcaa agagcacaag caagaaccgg 42900

ggacgatcaa cttcacaggg cgaatcagaa agtgaagccc gaagagcact ggtgcttcca 42960

caggaactgg gaacgctgga ctttaaagaa gagtttatca tcctgaaggg ggagaaccct 43020

gttaaagcag aaaaggcact ttattacctt gatccgtatt ttatggacag gttaatgaag 43080

gtcagtccta aactggcatc attgacgatg aaactaaata agacgaaaaa aatatttggt 43140

gtgaaagggc ttaaatatcc gtcaaaagaa aaaatgctct ccgtaggaga gctggagtct 43200

gaggttttgc tatgaaaaaa atacttatga tcagtattct tgtcctcaca gcctgttcct 43260

ctccacctga accgccacag gttgaatggg aaaaaaggcc tgaagttatg aatacacaaa 43320

taatgaactg gaaaccaaca tccggtgtta ttaaatcaaa taatataaat tcctcatgga 43380

gtaaggtgtt gcctgatttt aaaccagaaa accatcttta cgatgattct gttttttatg 43440

ccgttgccca ttctgaaaaa atagttgtaa ggacatcttc ttttgatagt tactggtcag 43500

cgaaagactg gctaagaaaa aatggtgcaa caggtgttat tgaatatcag ccactaaaaa 43560

gatggttgaa taatgactat gttgaaattt atctgtcaag aataaatatt cagaggttac 43620

tatgaaaagg atatgcaaag gtctaatcgt aatttttact gtgtcctgtt ttatagcgcc 43680

aacgtatgcc gctgatccct gtaaatccgt tttttgtctt tatggtaaag ctgtcggtag 43740

tagtggaggg agtgagtgca gcagtgcaga aaaggatttt tttaaaaatg ttgaaaagaa 43800

aaaggggaaa atccgctggg gtaaaacatt tgatctccgt aagaattttt taaatcagtg 43860

ttcaacggca gacccggctg caatctcact gatcatgagt aagtttggtc gcgtcagagg 43920

ttgattaaaa cctgataccc gcgccaacgg gtatcagggg actgcaagta tcacataccg 43980

cgccaacgaa atgtgatgat tagttacaat cactgaggtt agaaatgaaa gtaaccaaat 44040

ctattatatc tattttattg tgcttatctg ctggtagtgc ataagtggat gtaataaaaa 44100

atgctcgtac ctttttgaaa ggaacccttg atctgagcgt gcaggaagca gatgaaaggg 44160

aagaacttta taagaagaac ggggcgcaac ctgactattt aagttacctg gaggattaac 44220

acatgcaata tgcattattt gatgggatgg aacgaaagtt tttgctggat gctcttgaat 44280

ttggtgttct gaaggactgg aaagaaaatc cggtaaaaga acttcctgat attgatgaat 44340

ctgttcaccc cttccatgtc tgttatggtg gatatttatt aaaccctgat gtttcagatt 44400

tagatattag cagaaaaata aaagaccaga caggattctg gctggcagct attgatgata 44460

cccgtatgga ttgtcattca atagcttatt atgatattca caccctccct ttaatttcgt 44520

gtggtcatca gaagatagtt ccttttgcag cgttaataaa agctgatgaa tgcatcattt 44580

caaaaattgc ttcgtattct ggttttgccg taacagcctt tttgagaatt aaagaccaga 44640

atatcgcaac gaatatactt aaccgtgagg gaatttttgc ctttaatggc tgtgagcaca 44700

gattcagaca acctgtaagt gaagataact ggcaacaggc agtgtcagaa gaacgcgcta 44760

tccgttgtgc caaaagatta attcaatgta aaggataaca aaatgagact ttttatcgct 44820

gaaaaacccg cagtagcaaa tgatattgtt aaggcacttg gtggcaattt tacccgccat 44880

gatggctggt ttgaaagtga taacgccatt gtgactaact gttttggtca tattatcgaa 44940

tcacaaccgc cggaaaacta taatcctgaa tacaaagcct ggaaggttga aacgcttcct 45000

ttacgtcttt atcccgtgaa gtatcagcct gttgaaagtg cagcaaaaca ggttaaaacg 45060

attctcgaac ttatcagacg tggagacgtg actgaaattg ttcacgctgg cgatcctgat 45120

gatgagggac agctacttgt tgatgaagtc ctggaatatg caggaaacac aaaacccgta 45180

aagcgcgttc tgataaacga caacacgctt ccggcagtga aaaaggcact ggcaaatctt 45240

aaagataatc gtgatttcaa agggctttac cttaaggcgc tggcgcgttc agttgccgat 45300

gccgtctatg gattctccat gacgcgtgct tacaccattc ctgcaaaagc cagaggatat 45360

cagggcgttc tgtctgtcgg gcgcgtccag acacccgttc ttggcctgat tgtgaatcgt 45420

acccgtgcta accagaacca taaatccagt ttttactaca ccatgaccgg agtttttcag 45480

cgtggtgctg atgttctcag ggcgaactgg aaaccaggtg aatttgctcc gctgaccgac 45540

cgtaaattac ttgataaggc gtgggcagac ggaacggcag catcccttgc aggaaaaccg 45600

gctacagttg aagcagcagc aactgatgat aaaaaaacgg ctgcgccgtt gccgtttaac 45660

ctggtcagac tccagcaata catgaacaag aagtttaaaa tgacggcaca aaaaacgctg 45720

gatattacgc aacaactacg tgaaaaatat aaagcaatta cttataaccg ctcagattgc 45780

tcatatcttt ctgatgaaca attcagtgaa gcgccgcagg ttatcgatgc cctgaaatca 45840

gtctttcctc agtcgctgga tattgattct tcacgtaaaa gcaaagcgtt taacagtgca 45900

aaggtgactg cgcatactgc gataatcccg acctccagtg tgcctgatgt taacgcactc 45960

agcaccgacg agcgcaatgt ttacctggcg atcgcacaac actatcttgt tcagttcatg 46020

cctgaaaaag cataccagga agtatcggtt gccattcagt gtggtgatga gtcgttctat 46080

gcccgtgcca gaaaaacaac tgacagcgga tttgaggcgt ttcttggcgc ggaaatcaca 46140

gacgaaggtg aatcagaaga taatgatgat tccgcttttg aactgctctg taaaattcgc 46200

acaggagaaa cactgacgac aaaagaagtt gttgttaatg agaagaaaac aacaccgctg 46260

ccgttattca ccgaagcctc cttgcttgct gcgcttgttc gtgtcgcgga ttt 46313

<210>2

<211>32950

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>2

aaccaagaca accacacata aaaacaatat gctcactttt taaccactgc gcgtaattgc 60

ctgatgttat ccttgtgctg ttccgcttcc tcgctcacta actcgctacg ctcggtcgtt 120

cggctgcggc gagcggtgtc tcctttctca aaaccagaag agttcagatc atacgatccg 180

ttgattgatc ctttttggat ctttcactga gaagccctga atcgctttct gtcgcttcgt 240

tcgatacaac catggtgaaa ccatgcgaaa tcgaaaaaat cgctcagaat gcgatacagc 300

gcgttttaga gggtatttac aaaacagtcg ttgccttgag gaaattttga tcaagattga 360

actcctatcc ggatcatggc aacagatcac agcaacaagg ccgctaaaaa gcgttgagcg 420

gcgttaaaca ccgtcaaacc gtagccgact acaacgaaac gggaaaaacg ctcagaacgc 480

gttacagagc gttttaaggc gatgtcggaa agagccatat ctcacctgtt ctgatgtttt 540

tctcaacatg agcactttta agagtgcttt taaagatgct attatgccaa tgagacaagc 600

ggagggctta tgagctatca aattctgaca accacagcgg ccagtattac tgacctgaaa 660

aaaaatccta tgggaaccgt agctgaaggt gaaggggacg ctgttgcgat cctgaaccga 720

aacgaaccgg cgttctattg cgttccacca aaactttacg cctactatcg ggaactcgct 780

gaagatgctg agttaaacgc tgttgctgat gagcgcatga aaaacccgga aattgtgaag 840

gttaacctgg atgacctatg aactggcttt tgaccgtaga gcactgaagg aatggcagaa 900

actcggccac accatccgtg aacaattcaa aaagaaactg gcagaacggc tggaaaatcc 960

acgcgtaccc gcagcccggt tacatggtca tgctgatcgc tataaaatca aacttcgtgc 1020

atctggctac agacttgtat atcaagtcat tgatgagaaa gtcgttttac ttgttatttc 1080

cgttggaaga agggaaagca gcgaagtcta tcagatcgca gatttgcgct aagattaatc 1140

atcaaaggat taactgttta ctgtcagaac aagagcatta aaaatttgcc acacctgcaa 1200

atgcaggtat aataaaacac aagaaaggga aacaggttct ctttcatcca gagcccaaag 1260

tgggcgggag gttaaaaatg caaaactacg ctaagtctgt agccacagag attttacgtc 1320

aacttggtgg taatcgtttt attgttatga ctggtgctaa aagtttttct tactttgatg 1380

aaaacggtga gtgcgggtta actttccgtt tgccgtccaa ttttgcaatg aaaggcatca 1440

acttagtaaa aattaaactg gattttactg atacgtacca ggtgaaattt tctcgtgtac 1500

ggggtgatga agttaaagat atttcaagat tcgataatat ctattgtgat cagttagcgt 1560

gtttatttac acaagaaaca gggttacata ccgtgttata gataaaaagg ggcattaagc 1620

ccctttttta gttattatga ggaataatat ggataaagat aagataatta agaaaaacag 1680

aggtaattac tcatatgtaa tcagaacgat ggatgaagat ggggatgcgg tttttcacgt 1740

cttaaaatat gttaagacga ttgataaaac taaaagcagg aaaacagtaa gaaaattgat 1800

aatggacgaa aaactcaacc tggcatcatt gatgcttctg gataatggga ttttgtgtga 1860

ttgtctgaca aaaggggatg aaaatgcaga ataaacctac acctgaagaa gtaaagaatg 1920

cacgggttgc ggcaggtctt actcttaaag aagctgctga tatttttggt tatcaactga 1980

attcctggca gatgaaagaa agtgcaggta aggccagtcg ttctttatct gttggtgaat 2040

atcagtattt attgctatta gcaaatatgc atccgtctta caggctggta aaaaaataac 2100

ttgctatacc tgcaaatgca ggtataataa aacacaagaa agggaaacag gttctctttc 2160

atccagagcc caagctgggc gggagctaaa aatgaaagta ttaactttta aaaatgatac 2220

tgtctctgtt ggtgatgtct ttgtatcatc ctggggctat gagcaaacca atgtaacctt 2280

ctaccaggtt ctttctgttc acggtaaaaa aaccgtcacc gttcgcgaga ttcgcgctaa 2340

ttcagaatat accgattcaa tggtcggctt taaaactccc gttttaaatg attttactgg 2400

tgaatgtttt aagcgccaga taaaagattt tggtgatgag ctggcaatca aaatcgaaga 2460

ttttgaaact gcgtataaaa ctctaccgga agaaaaacat cgattttctt cttactactg 2520

attaatcagg gggatattcc cccttttagt aaaggtctga aaatggaaag agagtttagc 2580

gcaaaagcat cattaaaccg aaatataaaa ttttggtttg agcaatgtgg gttatctaaa 2640

gaaagagtta ttcgttgtat tgataactgg tatgaccttg catacccacc atcagaacag 2700

gagaaagcaa aaaaagaagc aattgagaag ttaataaagt aaagataaag atggtatcag 2760

gttgtggggc aacctgatac cgtgacacct gattctattg cgaggaataa aatgtcggaa 2820

cgcatattat cagcaataaa tgacgttgaa aagggtgggc gtccggtttt ccctttgatg 2880

ccattccatg tctttcctga gtatatggca ttactcagaa aagcactgga aaaaaagaca 2940

caaaagagaa cagataaata aaccggaggc tttatgacaa aacatgattt tgtgtccttt 3000

gtcagtggag aattgcggca aggcgctgta cgcttttctc ttgcttttaa cagcaaagga 3060

gaaattgttc tgcactggac taataaagca ggaatacgag tatggcggat attaagtggt 3120

aatcgtggca aaaaaccaag caaagctaac cttgaaagaa tgagtaactt ccggcgctgg 3180

ctttttgatg cccgtcaggg catggaaggc tacactcagc aacctgaaca gagcaacctt 3240

agctgaatag ccgaagccct cccgtatttc gggaggtgct ttctgatata ttgcgcggga 3300

ctgcgcttgc gcttcctgcc agaccgttcg cggagcgaac ctgacggcac tgtccttacc 3360

gcattgatcg ggtttccggt cgcagacgta cgtatgttca gccatgacaa catcagcctt 3420

tttgcatttt tgttgatgct caagcacaat ttatgagcgg tgccagattc actgcgatgc 3480

aggatagggc aaacgccgca aaatgacgtc tctgacgcca ttccgcaacg tttgcccggt 3540

gacctacagt cggttattgt cggagaattt tatgaaaaaa gttcaattca gaattgatga 3600

aaatcagcat aatgatttgc tggattgtct taaaactctt tatccagatg aaccagcttt 3660

aacagtagct aaaggcatga aacttttagc aaatgcttta ttaaaaagta aagctggcag 3720

taaggacata aatacgtttt ttgataataa tgattttatc aaaacaacga tgtacttaac 3780

aggtaaacaa agggctgata ttgaaagagc tgctaatcgt cacggatgga cgttatcacg 3840

agaatgtcgt taccgcatac agacgacact tgaaaatgaa ctggatttct ttgaccagga 3900

actgctgatg atgaatcgtt gccgtaattc aattgataag atcggtcgta atttccatta 3960

tatcattgtt aatgatcaga ccagggttct tgataaagat ggtttctatc aggatgcgga 4020

gcgtcttaca acagaaattt ttaatcttaa gaatcagttt gagaattaca ttatgttatg 4080

taaagggaga actgtttcaa ataaagtgga gatgtaatta tgggcgttta cgttgataaa 4140

gaatatcgtg ttaaacgaaa gtcatcagaa aatggtcgta agtcagcttt cgctcacaaa 4200

gtcaaaaatg gtggaaagaa ctatagccgc aatgttcagg aacgtatcaa ccgcaagggt 4260

gccagtaagg aggttgttgt caaaatatct ggaggtgctg ttactcgtca ggggattcgg 4320

aacagtattg attatatgag ccgtgagtca gagctaccag tgatgagtga aagcggtcgg 4380

gtatggatgg gtgccgaaat tctggaggct aaagagcaca tgatagatcg tgctaatgat 4440

cctcagcatg tgatgaatga taaaggtaaa gaaaataaaa aaatcacaca gaatattgtc 4500

ttctcgcctc cagttttagc gaaagtaaag cctgaagatt tgttggagtc tgtcaggaaa 4560

acgatgcaga aaaaatatcc taatcaccgt tttgttcttg gataccactg tgacaagaaa 4620

gaacatcctc acgttcatgt tgtttttcgt atccgagata atgacggtaa acgcgctgat 4680

atcaggaaaa aagatttacg ggaaattcgt acaggttttt gtgaagagtt gaagttaaaa 4740

ggttatgacg ttaaagcgac ccataagcaa cagcatggac ttaatcagtc tgttaaagat 4800

gcacataata cagcaccaaa aagacagaaa ggtgtttatg aggttgttga tattggctat 4860

gaccattatc agaacgataa aacaaagtct aagcaacatt ttataaagct aaagactctt 4920

aacaaggggg ttgagaaaac atactggggg gctgattttg gggacttatg ttcgcgggaa 4980

agtgttaaag caggtgatct tgtcaggctg aagaaacttg gtcagaaaga agtaaaaatc 5040

ccggcgctcg ataaaaacgg tgttcagcat ggctggaaaa cggttcacag aaatgagtgg 5100

cagttagaaa atctgggggt taagggcgta gacagaacac cttcagccag caaagagctg 5160

gtacttaaca gccctgatat gctgctgaag caacaacagc gaatggcgca gtttacgcag 5220

cagaaagcat ccacgttaca gtcagaacag aagctgaaaa cagggattaa gtttttgggc 5280

ttataagatt taatgtattg attatgcgag aaaaatattt attttagttc gcttgctcat 5340

cttttcacct taaaaacaga tcgaaattct gcgttttagc tatcattcga tctatcgatc 5400

taaaaaacag atctgcaaaa gatgaatatt ctgtgtttca ttgcatacat ggaataaatg 5460

aaagtaaaat tgatttatca agatcgaaag atctgtttta tgtgttgaac ttagcataag 5520

ccacttgtag tataaacttc gcgcaatagt aaaaagtctc tcttcatagg ggcgtgattt 5580

ctcacaagca accacggttg gcttgtgttt tgtcacatct gtatatcggg gattgagtat 5640

gaaaggacag aaacagtttt acctgcttca gtgttctccg cattttcatc agtcggttct 5700

gttgcagtta tcccggagcg gtatcgctta ctacaatcca gttatacgca ctttctataa 5760

gaggcgtgac tgtagtgcat acaggggaca aatcatgcca atgtttcccg gttatgtatt 5820

cgttcttctg gattttgagg ttgtacatcc atcctgcttt acccggatga aaaatgttta 5880

tggactggtc agttttggtg aatatcctgc tgaagttcct ctgagtgtca taaatgaagt 5940

aaaagagcag gagaagattt tttcgatgaa tttaagcctg atgaataact cctgtctggc 6000

aaaaatactt ctccttgcag acgcttcaaa gcgtggacga gtctttgccg actatgtgtg 6060

taacagaaaa tacgacaggt taaaaaatga cagctataaa aggaaagcgg aaaccgcaac 6120

gcaatgtact ctaccttcct acagaagttc gtgttgaagt tgaaaaaatt gcaattgaaa 6180

taagcttcaa gagggggcga cgtatctctg attctggttt tgttcagtat cttattaaaa 6240

aatacaaatc tcaggcgatg aaagaactta ttcatggggc tgatattcct gacgagtaat 6300

attgtcccaa tgttctgaat gtggaatatt tatgttatct acttctactt ttcttgcgct 6360

tgccatgcaa tgcgctgcca gcgttcatcc cgacacaacg cacgaagtcg ccagggttga 6420

atcaggtttt aacccatatg cgattgcgga aataatacca aaggttaaac gtaaaactgg 6480

tgataaaggc gtagtatctt actttcctga atcaaaggag gcagcactta agatcgttaa 6540

aaacattgaa ttacggaatc atcgttactc tgtaggactt atgcaaataa cgagtaccaa 6600

ttttgcaaag ttcggtacaa cagcagagaa aatgtttgat ccatgcgaaa atcttaaggt 6660

atcagaaaaa atactggttg actgttataa acgaggtggc gacttagtgc gtgggctgag 6720

ttgctattat tctggcaatc aagaaacagg agtaaagcca gaacctgaat ttaataatac 6780

aagttatgta caacgtatag gatttagccc tcctgataat aaaaaaagtt ttattgttcc 6840

ctctgtaaag gaaatgatta aaaaggagaa taagacgact atcacacctg aagaaatcat 6900

tatatatcct caatacgcca tgcgtggcac tgtatcaaat gaaaaggaaa caaaagatgt 6960

tgaaattaaa tctgaataaa cgttatttaa cgctttctct atttatggct gcgttgatgc 7020

tttgtgttgc agaacctgct tttgcggatg atgtgtctac gaagacaact ggttttttac 7080

agaaaataat tgatttttta acggatattc gtaaacctgc aattacaatt attgctcttg 7140

ttattgggta tattgcgata ttttctcgtc aacatacctc atggatagtc ccacttgtta 7200

tcggaattat catctttatt gttgcaccat atattcctga ctggcttgcg taattaaagg 7260

atgggcgcac tatgagtact ctttttaaag gtcttacgcg ccctgcttta ataagggggc 7320

tgggcgttcc gctctacccc tttcttggaa tgtgcattat ttgtgttctg cttggtgtct 7380

ggattcatga ggctatgtat gcccttattc ttcctggctg gtatgccatc aggcgtgtaa 7440

cacagtttga tgaacgcttt tttgaccttc tgtatctgag aactcttgtc aaagggcatc 7500

ctttatcaaa caagcgattc agcgcagtcc attatgcggg gagccagtac aatgaagttg 7560

atatttcaaa agtggataac tttatgaagc tgaaagacca gtcttctgtt gaagagttaa 7620

ttccgtactc ttcacatatc actgataata ttatcgttac aaaaaaccgg gatttgctgg 7680

caacctggca gatagacggt gcttactttg agtgtgttga ttctgaagat ttgtcaattc 7740

tgacagatca gcttaatacg cttatacgta gctttgaagg gaaatttgtt acgctttatc 7800

ctcatcgtat caggtgtaaa aagggcgtca gaccagtatt taacagtaaa attccttttg 7860

tgaacagagt aatgaatgat tattacgagt cattccctca gtctgaattt ttcgagaata 7920

aattatttct gacgatttgt tttaaacctt ttactacgga agataaagta acacatttct 7980

tttcacgcag taaaaaacaa aaagatatct ttaaagagcc tgttaatgaa atgaatgaaa 8040

tttgcgacag gttgaatacc tatctgtccc gttttcattc ccgacgtctt gggctttatg 8100

aagatcatgg ggttgtttat tcagatcagt tatctctgtt ccagtatctg ctttctggtc 8160

gatggcaaaa ggtcagggtt agcagtagtc cgttttatac atatctggga ggaaaagacc 8220

tgttctttgg taatgatgcc ggacaaatta ccgcgtcaga ccatgcccgg tattttcgtt 8280

gcatagagat taaggattat tttcaggaga cggatgccgg tattctggat gctctgatgt 8340

atctccccgt tgagtatgtc gtgacatcgt cctttactgc gatggataag cagtcagcga 8400

ttaaggcgct ggatgatcag atcgataagc tggaaatgac agatgatgct gccaaatctt 8460

tgctggcaga tctgaaagtc ggactggata tggtttccag tggatatatt tctttcggaa 8520

aatcgcatca gaccctggtt gtctttgcgg attcaccgga gcggctggtg aaagacacca 8580

atatcgtgac ttccactctg gaagatttgg ggctgattgt cacttattca acactgagtc 8640

ttggcgcagc ttattttgct cagctaccag gaaattatac gcttcgccct cgtctgagta 8700

ccctcagtag tcttaatttt gccgaaatgg aaagttttca taatttcttt tcaggaaaag 8760

aaaaaggaaa tacctggggg gaaaaactga ttactcttcg ggggtcaggt aatgatatct 8820

accatctgaa ttaccatatg acgactgaac atcagaattt cttcggtaag aacccgacgc 8880

tggggcatac cgaaattctc ggtacgtcta acgtgggtaa aaccgtatta ctgatgacaa 8940

aagcatttgc cgcccagcag ttcggtacgc cggaatcatt ccctgcaaac agaaaactga 9000

aaaaactgac cacggttttt tttgataaag accgggcagg tgaagtcggt atacgggcaa 9060

tggggggatc ttattaccgg gtgaaggagg gagagccgac aggctggaat cccgccgcac 9120

tgccgccaac aaagcgtaat atcgctttta tgaaggacct ggtgaggctg ctttgtactc 9180

tcaacagtga gccgctcgat gattaccaga acagcctgat ttcagatgcg gttgaacgtc 9240

ttatgcaacg gtcagatcgc tcttatcctg tcagtaaact acggcctctt atccaggagc 9300

cggatgatac tgaaaccaaa cgtcatggac ttaaagcccg tcttaagccg tggacgcagg 9360

gggaagagtt tggctgggtg ttcgacaatc gggaagacac gtttgatgtc gataacctgg 9420

atgttttcgg tattgatgga acggagttcc tggataataa ggtgctggcc agtgctgctt 9480

cattctatct catctatcgg gtcaccatgc tggccgatgg tcgcaggctt cttatctaca 9540

tggatgagtt ctggcaatgg atcaataacg aagcgttcag ggactttgtt tacaacaagc 9600

tgaaaaccgg acgtaaactc gatatggtgc ttgtcgtagc cacacagtca ccggatgaac 9660

tgattaaatc acccattgcg gcagcggttc gtgagcaatg cgccactcat atctatctgg 9720

caaacccgaa agccaaacgt agtgaatatg ttgatggttt gcaggtcagg gagctttatt 9780

ttgacaaaat taaagctatc gatccgctgt cccgccagtt ccttgttgtt aagaacccac 9840

agaggaaagg tgaaagtgat gattttgctg cttttgccag actggagctg ggaaaagcag 9900

cgtattactt accggttctc agtgcatcaa aaccccagtt agaactgttc gatgaaatct 9960

ggaaagaagg aatgaagccg gaagagtggc ttgataccta tctggaacag gcgaacctga 10020

tttgaggaat caccaatgaa aaagcaaatt atggcggcat tcgtcgcttc actgattgtt 10080

atttccggcg ctcaggcagg gatacctgtt gccatcgacg ccaaccctga atgggcgatt 10140

gaagccggac gatggacaga acgccttaag caatgggcgg aaacggtaaa acattacgaa 10200

aatcagataa atgcgtacaa acaggagctg ctgtcaaaaa cgggtatccg tgatgtgcag 10260

ggactggtgc agtccgcaca gtcagtgagt caggaactga tgcagattta tgatcagggg 10320

aatgctttta ttgacgatta cattaaaaat cctgaagggg cgttatcgga acaggccaaa 10380

tcgttattgt cagattacaa agtaacggat acctgccaga acctgggata ttccggcgac 10440

ctggtacggg gatgtgaagc gacgttcctt tctcaactgg caagcgtgga atacggtaac 10500

aagctggaga gcaagcttcg tcaggacaac cagacgatga aagaccttat tgatcaagtc 10560

aaaaatgcga aggatacgaa ggccacgcag gatgcaacaa acgctgttgc acttgaacaa 10620

ctgaagttcg agaagctcaa atttcagtat caaatgtatc gcgataagca gcgagatctt 10680

gcagaatata aagagaagat ggctcaggca gctttcagaa aacagcaacg tgaagccgtg 10740

ccaccttctt acagaaaagc ttatatggca atgaaatcat atgaggatga ttaaatgaaa 10800

agtatcgcta ctgctggcct tgtcattata tctgttttta gtctttctgg atgttttgaa 10860

gaaacaaaat cggttgattg gtggcttgca catccgaagg agacatacaa aaaatttgaa 10920

gagtgtcaga agtctggtag cgattctgat aattgtaaga acgttaagcg agcacatttg 10980

tcttttgaac ggagaaaggc ggttggttta ccaataaatt gagggtgatg tatggggatt 11040

gtcactggta taccggatgg tactgattta tcaggtaatg taacctctcc cgcaaatgcg 11100

ggaggtgttt ctggatttga ttctgatttt tttcagacaa ctcatgaagt tatatttaat 11160

attctcaaca agagtatatc tggaaaatta agtgaatatt cagatgtggc ttatactctt 11220

ggtaaatatg gagtttctct gtatgtttta tggtatgctt ttactgtatt agcaaggaaa 11280

caacagacac ctgtacctga ttttatctgg aatatctgta ggttttacat aatattgctt 11340

tttgttaaga atacaggggg aatacttaca tcagcaacag atgctattga tggattgaaa 11400

aatacattgg cagggggaga tccgtgggta tggatggatc agttatgggt gaaggttata 11460

caagttgcaa ctcttatttt tgataaagat acatctactg tgcctgttgc cggtgggatt 11520

ggtgctttat taacttatgt cggtggtgtt ttggcattat tgctttgttc tatagtattt 11580

gcatctgctg aattaacatt actattactt tctgtcactg cgccaatatt tatcatgtgt 11640

ctgatgtttg gtttacttcg gcaaatgttt aatagctggc tacagcttaa ttttagctcg 11700

ttactggttt ttctatttgc agcattagca ctaagagctg ggacatggca attaaacatg 11760

gcattaagta cgtctattgc tacagcatca gaaaacaatc ttcttcaaac gggagtaact 11820

tcattagctg ctggcatttt catggcctgg attatctggc aggcgaaaag ttatgcttca 11880

cagattgcag gtgtgggtgt tgaaggtgcc atgcagggcg cagccgctat ggggattggt 11940

gctggcgttt tcggtgcatc ccgtatggcg cgtggcgcac ttggtatggg cagaaatgcc 12000

ggtattggtg catggaaagg tctgcgtcgt caggaagacg ggtttggtca gtctccggga 12060

ataacgggta agaccgctaa cctggccgga cagggcgtta atattggtgc caaaaagctt 12120

cgtcaggcag ctattgagag agcaaagaaa atgtatggtg ggtaatttaa ttatgagcgc 12180

cttttaatgt tcttttcata attgtcattg tcgggcctgg cgcgctctat tatcttttct 12240

gttcacactg agtcttctgt aaatattaat ctgttataca ggtgttcata ttatgaaact 12300

acttattgtg gctttcgtga cgctttgtct cgctggttgt caggcgtcac ataaactacc 12360

gcccgtttcc gggaaaagcg aacctgttaa ttctgctgag gtaatgcaaa atggaattta 12420

aacttcccgg atttaaaaat aaaaaagacg ttactgactc atcagtttca tttgaagaaa 12480

aaaacattgc actacaggag agaatgaatc gtatttataa attcggtggt atcggaggca 12540

tgttaattgg tgggctgtct ttacttgcat taaatgcagc attaccactg aaaacaacag 12600

ttgttgatgc ctaccttatc gataaggtta caggtgtggc tgaacgtctg acttctgtta 12660

aaaaagaaaa tctttctgaa aacgaagcca ttgcccgata ttttatcacc cagtatataa 12720

aacatcgtga aggttataat tttttcagtc tccagcatga ttatgattat gtaatggctt 12780

acagcgcgga gaatgtcgcg gcagattata acgcattatt taacagtgaa caggcaccaa 12840

aacttgttta taacaaagca gaaaaaacgg caatggttca ggataatcca tctgtcataa 12900

tttcaccttc gtcacgggca gatgataaag atatcggtgc gtatattcgt tttcgtctga 12960

ccatcaggga tgttgctacc ggacaaaccc gccaggagtt ctggaatgtt cgcctgactt 13020

atcgtatcga accgcaggtt gaaatggtgt caggggaacg taataacaat cctcttaaat 13080

tcgttgtaac aagctacgtt cgcgataaag aagccagagg ttaataatat gaaaatgaat 13140

aaaggagcgt taattatggc gcttctgatg gcggcgcacg tctgtcatgc agctgttctt 13200

ccttcaggca gtcgctttga cccacgcaat cagatagtca gttataaccc caataatacc 13260

accataatta acagtgccgt tggatacacc accacactgg tatttgatga agatgaaaca 13320

gttatcagtg ccagaactgg ttttccgcag ggatgggcgg ttaataaaga agataacctg 13380

gtatacctgg aagttcgtcc tgttaaacag actgttcaga aaaataatat ggatgaaaac 13440

ggtaatacct cttctgaatc cgtcagtgtt gctcttgacc cggaaaatga gcttgaacgc 13500

tggcgaacga atttgtttgt tcgcaccacg aagcgtaatt acagcatgga gctgaacgcc 13560

cggacgttcc ggcagccgga gaaaattgcg tttgtggtga attaccagta tccgcaggaa 13620

cgccggaagg aacaggccga aattgagaag aaacgcacag aggctcttgc cagacgccag 13680

gaggagcagg caatcaaccg ttccctggaa aatgcgaaat cgccccgtaa ctggcagtac 13740

tggaagcggg ttgctgaagg cagccaggat atcagccctg attatgcata tgacgatggc 13800

cgttatacct ggttcggctt cagtccgtta aagaaaattc ccagcgtctt tgtgatgaac 13860

ggtatgcagg agactcttac caatcctgtg attaaacaga gcgggagttt tacggctgtt 13920

ggcgtaccgg ttgataagcg ttttgtttta cgtcttggtg agcaggtggt ggggattgag 13980

aaccagggct tcggaaaagt acgtttacca gccggagata cggtatcccc ggatgttaag 14040

aaagaggtga tccagtgact gaacaggaaa ataaaatccc gactgcaacc gaaattgaac 14100

agcagctacg ggaacgcaga cagaaagaac tggaacaggc cgggaagact ccggaagaag 14160

agcctggcaa gccagcattg cagcttggta ttgaaaaact taaaaagtca cgtaaaggga 14220

tgattatcct cgtcgtgggt tttcttctgc ttgctgccgg tgtttctgtt tattatatcc 14280

cgtccattat ccgttctgtg tcgtcagggg atgagaaacc cgcaagtcag ccgcttgcaa 14340

ccggaacggc taaacgtcag accggactga gcgaagatat cgatcctttt aataccgcac 14400

agaaaaaaac agagaaacca gaggaagaaa aagtcatttc ttctgaaaag actgaaccgc 14460

cggaaaataa acagcagagc ttcagccgtg cacttgacgt ttctcttgat ggcagccaga 14520

caggaaacag cagcagttca gcgggaacgt cagtttcaca tactgcggcc agtgagccag 14580

aaagcgataa aaaggatgaa gcaaaagcaa ccgcacagac tacagaatct gcgccactgg 14640

cgaaaataac gaaacttcca tatgacccaa atttgtttat cccggaaggg acatcaattc 14700

cctgttcact ggacaggcgt tttgtttctg acctggcggg gaaactggaa tgtacggtca 14760

acagcgatat atacagcgcc agcggtaatg taaaacttat cgaaagagga accgccgcaa 14820

aactgatgta taaggccggg tctttaaatc atggacaagg gcgtgtgttt gttatggctt 14880

acaagctacg tacccgcagt aagcctttta ttgatattcc cctggttgac tcacaggcgg 14940

ctggcgcgtt aggcgaagcc ggtgcttctg ggtggattga cactcatttc agtgaacgtt 15000

ttcttggtgc aatgatggtc gggatgatac cggatttaag tcaggccgcc agtggtattg 15060

cacagaacaa cagggacagc cagaccgact atacggcaaa cagtcgccag gcttttgctg 15120

atatagcacg cgaagcattt tctaatagtg tgaatattcc gccaacgctt tataaaaatc 15180

agggcgaaat tattactctg attgtcggtc aggatctgga tttttcaggc atttataaac 15240

tgaaaatgaa agggggttaa tgtgaataac gaaaacagac atctgattta tgatgtggtc 15300

aacgattatt tttatcactg gctgaatgag attgagggtg tcacggaaat tgctgttaac 15360

cgaccaggag aaatatttat aaaggtcagg ggaaagtggc aatggtatga acaaaagatg 15420

agttacagtg attgtctttc ttttgcatcc acactggccg attttcatga cggcggttct 15480

gtgactcctg aatatcccct gcgctctgcc acgcttccgg gtggagaacg tgttcaggtt 15540

gtgatcccac cggcaactga aaaagacact gtttctataa caatccgtaa gccgtcaggt 15600

atttttatca gtcatgacaa atttataaaa cagggatttt attcacgcgt cagtggttta 15660

agtggtgact cggttatgga agataatatt tctgctttaa tcacttccgg atattttgat 15720

cgggttatac ctgaatcact gcgtcagggg aaaacgatag ttttctgtgg agggacgggt 15780

tcaggtaaaa ctacctttgc aaatgcctgt ctggaatata taccgcatca tctgcggtgt 15840

atttctattg aagatactga tgaggcaaaa ttcagattcc ataaaaacca tgtaaaactt 15900

tactatccgg cagagggtga gagtaaggtt attacctcag cgagtcttct gcgttcctgt 15960

tttcgtatga atccggacag gattctgatg acagaaatca ggggggctga ggcatgggat 16020

tttctgaaag catcgagttc aggccatgca ggaaacatta ccaccgttca cgaaagtagt 16080

cctgaatatg ctgtgcttgg gattgttcag cgatgttata tgaatcctga atgtcagaat 16140

ctaccattca atgtcatttt aagacgtgta ctgagtaata ttgatattat catgagtatt 16200

aaataccttg atgatgaaga ttttcgtttc gcttccggta tttattacaa acaacttcat 16260

tttgatgact atttcagaaa actgaaggag tgattatgtc tttaaaactc cccgataaag 16320

gccagtgggt ttttatcggt ctggttatgt gtctcgtgac atattatact ggttctgttg 16380

ctgtttactt cctgaacgga aaaacgccgc tttatatatg gaaaaatttt gattccatgc 16440

tcctgtggcg aataataaca gagagtaata tacggacaga tatcaggtta accgctatcc 16500

cctctctttt atcaggtatg gtttcgtctc tcatcgtgcc tgtttttatt atctggcaac 16560

tgaataaaac ggctgttgct ctttatggtg acgcgaagtt tgccagtgat aatgatttaa 16620

ggaaatcgaa acttctgaaa tgggagaaag aaaacgatac tgatattctc gtcggagcat 16680

ataaaggtaa atacctgtgg tataccgcac cagattttgt atcacttggc gcaggaaccc 16740

gcgcaggtaa aggtgccgcc attggtatcc ctaatcttct ggtcagaaaa cactctctga 16800

ttgcgttaga tccaaaacag gaattgtgga aaatcaccag taaggtgcgt gaaatactgc 16860

tgggtaataa agtttatctg ctcgaccctt tcaacagtaa aacacaccag tttaatcccc 16920

ttttctatat tgatttaaaa gcggagagtg gggctaagga tctgcttaaa ctgattgaaa 16980

ttctgtttcc gtcttatggc atgacagggg cagaagcgca ctttaataat cttgcgggtc 17040

aatactggac aggactggct aagttgcttc atttctttat taactatgag ccgtcctggc 17100

ttaatgagtt cgggcttaaa cccgttttct caatcggttc tgtcgtcgac ttgtacagca 17160

atattgaccg ggaactgata ctcagtaagc gggaagaact ggagggaaca aacgggcttg 17220

atgaaaacgc gttgtatcat ttgcgcgatg ccctgaccaa aatcagggaa tatcacgaaa 17280

cggaagatga acagcgttca agcattgatg gttctttccg taagaaaatg agcctgtttt 17340

atctcccaac cgttcgtaaa tgtactgatg gtaatgattt cgatctccgt cagttgcgac 17400

gggaagatat cactgtttat gtcggtgtta atgcggaaga tatatcactg gcttacgatt 17460

ttctgaacct gtttttcaac ttcgttgttg aagtgacatt gcgtgaaaat cctgattttg 17520

atcccaccct gaaacatgac tgcctgatgt ttcttgatga gttcccttcg attggttata 17580

tgccaattat taaaaaggga tcagggtata ttgcaggttt taaacttaaa ctgctgacaa 17640

tttatcagaa tatcagtcag ctaaatgaaa tctatggtat tgagggagcc aaaacgctga 17700

tgagtgctca tccctgccgt attatctatg ctgtcagcga agaggatgat gccgcgaaga 17760

tatcagaaaa acttgggtat attaccacta catcaaagag cacaagcaag aaccggggac 17820

gatcaacttc acagggcgaa tcagaaagtg aagcccgaag agcactggtg cttccacagg 17880

aactgggaac gctggacttt aaagaagagt ttatcatcct gaagggggag aaccctgtta 17940

aagcagaaaa ggcactttat taccttgatc cgtattttat ggacaggtta atgaaggtca 18000

gtcctaaact ggcatcattg acgatgaaac taaataagac gaaaaaaata tttggtgtga 18060

aagggcttaa atatccgtca aaagaaaaaa tgctctccgt aggagagctg gagtctgagg 18120

ttttgctatg aaaaaaatac ttatgatcag tattcttgtc ctcacagcct gttcctctcc 18180

acctgaaccg ccacaggttg aatgggaaaa aaggcctgaa gttatgaata cacaaataat 18240

gaactggaaa ccaacatccg gtgttattaa atcaaataat ataaattcct catggagtaa 18300

ggtgttgcct gattttaaac cagaaaacca tctttacgat gattctgttt tttatgccgt 18360

tgcccattct gaaaaaatag ttgtaaggac atcttctttt gatagttact ggtcagcgaa 18420

agactggcta agaaaaaatg gtgcaacagg tgttattgaa tatcagccac taaaaagatg 18480

gttgaataat gactatgttg aaatttatct gtcaagaata aatattcaga ggttactatg 18540

aaaaggatat gcaaaggtct aatcgtaatt tttactgtgt cctgttttat agcgccaacg 18600

tatgccgctg atccctgtaa atccgttttt tgtctttatg gtaaagctgt cggtagtagt 18660

ggagggagtg agtgcagcag tgcagaaaag gattttttta aaaatgttga aaagaaaaag 18720

gggaaaatcc gctggggtaa aacatttgat ctccgtaaga attttttaaa tcagtgttca 18780

acggcagacc cggctgcaat ctcactgatc atgagtaagt ttggtcgcgt cagaggttga 18840

ttaaaacctg atacccgcgc caacgggtat caggggactg caagtatcac ataccgcgcc 18900

aacgaaatgt gatgattagt tacaatcact gaggttagaa atgaaagtaa ccaaatctat 18960

tatatctatt ttattgtgct tatctgctgg tagtgcataa gtggatgtaa taaaaaatgc 19020

tcgtaccttt ttgaaaggaa cccttgatct gagcgtgcag gaagcagatg aaagggaaga 19080

actttataag aagaacgggg cgcaacctga ctatttaagt tacctggagg attaacacat 19140

gcaatatgca ttatttgatg ggatggaacg aaagtttttg ctggatgctc ttgaatttgg 19200

tgttctgaag gactggaaag aaaatccggt aaaagaactt cctgatattg atgaatctgt 19260

tcaccccttc catgtctgtt atggtggata tttattaaac cctgatgttt cagatttaga 19320

tattagcaga aaaataaaag accagacagg attctggctg gcagctattg atgatacccg 19380

tatggattgt cattcaatag cttattatga tattcacacc ctccctttaa tttcgtgtgg 19440

tcatcagaag atagttcctt ttgcagcgtt aataaaagct gatgaatgca tcatttcaaa 19500

aattgcttcg tattctggtt ttgccgtaac agcctttttg agaattaaag accagaatat 19560

cgcaacgaat atacttaacc gtgagggaat ttttgccttt aatggctgtg agcacagatt 19620

cagacaacct gtaagtgaag ataactggca acaggcagtg tcagaagaac gcgctatccg 19680

ttgtgccaaa agattaattc aatgtaaagg ataacaaaat gagacttttt atcgctgaaa 19740

aacccgcagt agcaaatgat attgttaagg cacttggtgg caattttacc cgccatgatg 19800

gctggtttga aagtgataac gccattgtga ctaactgttt tggtcatatt atcgaatcac 19860

aaccgccgga aaactataat cctgaataca aagcctggaa ggttgaaacg cttcctttac 19920

gtctttatcc cgtgaagtat cagcctgttg aaagtgcagc aaaacaggtt aaaacgattc 19980

tcgaacttat cagacgtgga gacgtgactg aaattgttca cgctggcgat cctgatgatg 20040

agggacagct acttgttgat gaagtcctgg aatatgcagg aaacacaaaa cccgtaaagc 20100

gcgttctgat aaacgacaac acgcttccgg cagtgaaaaa ggcactggca aatcttaaag 20160

ataatcgtga tttcaaaggg ctttacctta aggcgctggc gcgttcagtt gccgatgccg 20220

tctatggatt ctccatgacg cgtgcttaca ccattcctgc aaaagccaga ggatatcagg 20280

gcgttctgtc tgtcgggcgc gtccagacac ccgttcttgg cctgattgtg aatcgtaccc 20340

gtgctaacca gaaccataaa tccagttttt actacaccat gaccggagtt tttcagcgtg 20400

gtgctgatgt tctcagggcg aactggaaac caggtgaatt tgctccgctg accgaccgta 20460

aattacttga taaggcgtgg gcagacggaa cggcagcatc ccttgcagga aaaccggcta 20520

cagttgaagc agcagcaact gatgataaaa aaacggctgc gccgttgccg tttaacctgg 20580

tcagactcca gcaatacatg aacaagaagt ttaaaatgac ggcacaaaaa acgctggata 20640

ttacgcaaca actacgtgaa aaatataaag caattactta taaccgctca gattgctcat 20700

atctttctga tgaacaattc agtgaagcgc cgcaggttat cgatgccctg aaatcagtct 20760

ttcctcagtc gctggatatt gattcttcac gtaaaagcaa agcgtttaac agtgcaaagg 20820

tgactgcgca tactgcgata atcccgacct ccagtgtgcc tgatgttaac gcactcagca 20880

ccgacgagcg caatgtttac ctggcgatcg cacaacacta tcttgttcag ttcatgcctg 20940

aaaaagcata ccaggaagta tcggttgcca ttcagtgtgg tgatgagtcg ttctatgccc 21000

gtgccagaaa aacaactgac agcggatttg aggcgtttct tggcgcggaa atcacagacg 21060

aaggtgaatc agaagataat gatgattccg cttttgaact gctctgtaaa attcgcacag 21120

gagaaacact gacgacaaaa gaagttgttg ttaatgagaa gaaaacaaca ccgctgccgt 21180

tattcaccga agcctccttg cttgctgcgc ttgttcgtgt cgcggatttt gtcactgatc 21240

caacgattaa aaaattactg aaagataagg ataaagacaa aaaagatgaa catggcggta 21300

ttggtacgcc agctacccgt gcagccattc tggaaacgct gaagaagaga aactatatca 21360

cgctggaaaa agggaaactt attccgactg ataccggata tgcgcttatt gatgccctgc 21420

caggtatagc ggttaatcct gatatgacag cattatggtc tgaaaagcag actgccattg 21480

aaaatggcga cctgacggtt gaacagttta ttaatgagct gtacggtgaa ttgacaggca 21540

tgatttctga tgttgacctg ggcaagatga agattgaacc cgctgcgcca gcagggcagt 21600

ttcaacgcct ggactctccc tgcccttcct gtggtaaaca tattgttatc aggccgaaag 21660

gttatttctg taccggatgt gaatttaaaa tctggagtga gttttctggt aagaaaatca 21720

cccaggcaca ggccgaaaaa ctggttaaat cagggaaaac cgatttgatt aagggattta 21780

aaaagaaaag tggtggaacg tatgatacag ttcttgtcct tgaggataag aaaacaggga 21840

agctgggttt tccggcaagg gctaagaagt gaaaacaaag caggaatggc tttttcagtt 21900

aagaaaatgt acatcaagag atactcttga aaaagttatt gagattaacc gttacaagct 21960

gcctttatca gaatcagagg cattttattc tgccgcagat caccgccgtg cagaactggt 22020

gatgaataaa ctttatgata aggttccttc cggcgtatgg aagtacgtcc attaaacaag 22080

aggattaatt atgagcgaac tgactaaaga agatgaatac ggcattatca gccggactat 22140

gatgaatatt cgttcattgc gtgtgtttgc ccgtgagatt gattttgagc agttgctcga 22200

aatgcaggaa aagctcaacg ttgttattga agaacgtcgt gaagatgctg aacgtgaagc 22260

ggctgaacga gcagagcgtg aacggaaacg tcaggaactg cttcagttaa tcgccggaga 22320

gggtttctca ccggaagaac tgcttggtct gtccgaagaa gcaccaaaat cacgtaaaaa 22380

aacgttacca aaagcgccac ccaagtatca atttgaagaa aatggtgaaa cgaaatactg 22440

gtctggtcgt ggacgtgcgc caaaaccgat tgatgaagcg ttgaaagccg ggcgttctct 22500

ggaagatttt cgtatcaata agagtttgaa cggagtaaca gatgagcagt aatatggcaa 22560

ggatatagtt ttatatcatc attttgttaa ggaagaaaat ccatgagtaa tacatcctac 22620

aaacaaatta tccctgcgac agactggtat ttccgtcacg ataatgtctc cggtgtggca 22680

ggaaagtcaa cagtatacca actggctgca tgggcgctta aagaaaatgg tgaggtagtt 22740

ggtctggtga cggttcgtga tgataatggg cgtcctaaac tggttactcc tccccctgtc 22800

cctggtgatt atttgcataa agaacaactc accgatgatg aaaaagagtg ggcgaagaga 22860

cgctaaacta tattcatata aagcctctgt tctagaggct ttattagcat gtttagttgc 22920

actggagttg ataatggctt caatgctcaa attgaggtaa attgatattg attttacttt 22980

tccgttgctc gtttctcgtc ttattatatt taaatgacaa ttgtaattgt ataatgtttt 23040

attactcttg aattgtctga gcctgatatt tttcaataat aatgttattg attcctaaat 23100

ctgatagcag ataagatgtt acaattgttt ttaggtagtt tccaatttta acataatcac 23160

caatcgtcaa tatgttcatt aactcctttt tgcggtcaac atgtgctaat tcatttctga 23220

gtgtggcaat attttcacca atagatttat tgttgaattt cttaaaaaac atctctatct 23280

cttgaataag aaacaaactt gcatattcat taatgggttt catgtatttt tcattttttg 23340

agccacctat tgttttgttt attgcttcta attgagtcgc aaacaatata atatcagtat 23400

gagcttgatg cagtgttcga aaaccggttt catactgata tgttattgtc aatggcatat 23460

atctttctgc tattttgaac catttgcaga atatctttcc aagattaata tgcttcctgt 23520

ttattggaag gagttgatgt tttatttccc tcagcgctag gtcaatagtt ctttgttcaa 23580

accccgttgt taagagacat ggtgtcttcg aatcatttcc cttgaacttt atgtttattt 23640

cttcaggaag agtcggtttg ttcagaagaa ttgaaaataa cccggaaata tcccagcact 23700

ttgatatatg gtcttttatt cctaaatcat tagatgattt tatcctaaag tagaaaacaa 23760

gttcttttct aatggagaaa aaagcatcag ggtataactc ttttgtcttt tttagttgat 23820

gaattatgtt ctccagtgcg gctttgtttt ggcaatttat tatgtttaat agatcatcac 23880

caattacgct aaaagataca tggttgacaa gttgaagagt ccaatggttt ccttttgcga 23940

taaatattgg atgttctaaa tgtttcaatt gagtgaaaaa accgtgaggg tggataaatt 24000

cttgcaatcc gtgcaaggat aaatcacaat attctatctt tgagtcgggg gcataaaaat 24060

cattgaatag cattatggga aatccatgcc ttccagtatg gataatccct ttaccaaaat 24120

gaatattccc ttgtgtgaaa tcaaatttcc caattagagt acatctttct ccagtattta 24180

agactccata taatatttca catgttctgg ggctttcact gtcagaaata caataatcta 24240

gaatcagacc atggtacggt gaatattcaa ttcgtgcaga aaatcttcct ttgttatcat 24300

caagttggtg ccaaaattca ccatgaaaaa agtattcttt tgttagttcg tatttttttg 24360

ttgccatgta ctgctccatt tgtttctgag aatccttcag aatagtaaaa atactactca 24420

aaactcgtct tcaagatctc tggctatctg ctcattttca tgatgctcaa cagcatcact 24480

gatcaaattc atcatgttat taagaaactc ctctacaact ttaaacgcat cattgagcgt 24540

agctgtattg aatgtatgta tcttttcatc cgttgttttc gttttcccgt tacgatgaac 24600

tatgtcatgc ctcagtttca tcaattcatt gatatttttg agtggaaaac gtggatattg 24660

cttaggttgc aggactgctt tatatatttc aactaccagt tggattctat gataaagaat 24720

atctgaaagg tactcctgta catatttgtt tgcattagac tctttattta tcaactcaga 24780

cagagagata ttctttgctt tcagttcatt tatgtttctt atggcatttt ctacatatct 24840

gttatgtgac aatacaacgc ttttaatcat ttcgctaaga cagttttcca ttattgtcac 24900

aatgtatgcg attttcattt ttatgaaaac ttcacttgta gattgttttc cctcttcttt 24960

tatttgttct aaaaggttta ttgctatttt ataaatttct gtatgtggat gctgttttaa 25020

ccatttttcg ttttcatatt cttccatagc catatcagaa agaaagtcct gatactcgtc 25080

atagtccttt tcaagtaact gccattcttc agaatcctcc tcaagatctg ggctggaaag 25140

ccgttctcgt atccattcct cacggcgact ttcctcgact tgcataaccc aatcttttgt 25200

agctcccaca ataccctcct tatgttcatt gctaaatatc aatagtcact ttatcggaga 25260

ctacgtgaaa aatcttctcc tttccccctg cttttttatc agatttcacg cagcgtaccg 25320

ccgctactgt atccatcaaa aaaactgata gctttttaat catcttttgt gctgcaactt 25380

actgaaaaaa ggattaatgg aaacgccgat aacggcattg atctgttggc caacgcgacc 25440

ggcagcattg gacttaacaa aaaaactgaa agaaaatccc ccaaatgaag ggaaaaaacg 25500

gaagaacagt cgaacgacag gcacaaaaaa acccgactgg taatcgggct ttttcgtgtt 25560

ttccagtcct gagttggtgg ctctgactgg aggaagatac tctacatcca acatccacaa 25620

tattatggca ctagacacca gttgtctagt ttgacttcag attgtctgtc aatggccatt 25680

tctttaaatg aattttatgc gtgcatctgt ataatctttg tgttggcgct gtacagtgtg 25740

cctcccttgc ttctaacccc tgcgctatct ggggatgaac gaattaaagt cctggctgaa 25800

caaacaggtg gtgctgtgtt cagtgctctg gcagggggaa ggtactctac atccaacaat 25860

gtgagagagc cgttgtgaca ggcgatgtat gagcaagcaa cggtgacttc ctccagtatc 25920

aacctagaga aacggatgat gaatctgtta atcatcgttc tcagggctgt gctcgccatt 25980

gcaaacgcgc tgattgccat tcagaaacta atcgagtgat tgatggctta actaagggag 26040

agacgtttac cgcttcctgc cctttaacaa aggagtgttt atgagttttg aacaattatt 26100

tacactaacg acccaacgtc gttgggtttg ccgcagaaaa tttattcatt taaaatagat 26160

ctaaatgaag cgggaaactg cagacgtaaa aaaaccgact ggtgaggtcg gcttttttac 26220

agttccggta cgagctggta actcgcccgg aagagaaact ctctacaact gacaacagta 26280

tgataatccg tgaagcctga ttatgcaata tgacaggcgg tgactacgcg ttaacggttt 26340

gcttgaaagt tagataaacg tataatcaga gggttagtgc tgtggtgagt ctttccctgc 26400

attccgctaa atgcggcatg aacagcttca aagtcctgac aggtaataca ggtggtgctg 26460

gttatctgtg ctctggtagg agtgagactc tctacaactg acaagacgaa gagaagccgt 26520

ggtgacaggc agcgtaagag caagccacgg tgtctttact ccatttatgg agaaaacgga 26580

tgattaacct gttaatcatc gttctgaggg cggtagctgc gcttgcaaac gcgctgattg 26640

cagttctgga actgatccgt cagttcatcg attgatgact gcggaaacgt aactaagggc 26700

aggaagtgca ccgcttcctg ccctttaaca aaggaggtgt tatggatttt atgagcattt 26760

tggggcgctg tttacttctt cctttccctg ttgcgatgtt tgtcggttgc ctgtttccag 26820

gcatcgatga tcgttttact ggaatgttca tgagttttat tgtcggttgt ctatcgtggt 26880

atatcaccaa gccaaaaccg caaaaggctg aggtcgcgta agcggccttt tattttgata 26940

cgcattagtt acaacattca atttgctttt taaaaatatc cacttcagca cctccctcat 27000

aaataacgtc aaccagttta ttgagctcac cctaccctac tcaccggagg attgcaggga 27060

tttacgtttt attgcgctac tgattgtgcc gcggctgcac cctagtacct tctgtacctg 27120

tgtccaggag ctgccacttt ctatcaacct gataattgca tcatgtctgg cttgattagg 27180

tttacgccct ttatatttgc cgtctttcct tgctttctct atcccctgtt tctggcgttc 27240

ccgccgttgt tcatagtccc ttctagcaac agcggccagc atatccagca gcatatcgtt 27300

aatggctgca aacaaccggc tgtcaaactc actcatacca gaattaatcc aggtcgtcgg 27360

cacattcacg gccataaccc ggatatcttt ctgacggatc attttcttca gcgtattcca 27420

gtcttcccct gacagtcggg aaagtctgtc cacatcctcg ataagcaaaa tgtcattttg 27480

ctggcaatct ctcagaagac ggaagagttc cgggcggtca agacgggagc cggattcatt 27540

ctcaatataa tagctgcaaa tgatcaggcc tctttccctg gcaaaagcct cgatagtttc 27600

cagggcacgc gaagcatcct gttctgctgt tgaagctctc agataagcgc ggacaaagtt 27660

tgttggtgca tttgaagtca tattacagaa ccagttcgca taaggtcaca ccactatacc 27720

caaaatgaac cagaaagtgg atcgacagga aaggcacact ctaaaagaac cgaaaaaaca 27780

aataatgagt aaatgtgaca tcgtcaccca tactcataaa ctcacatgct catatacaca 27840

acaaactcaa atgagtatgt gctctttaac tcataaactc atattaacct ttactcatgc 27900

aaagagtatg gtaaactcaa aaactcacaa gagtaattga gtagatacac aattgagggt 27960

ttgagtatga aagtaatctc atttctgaat ccgaaagggg gttcaggtaa aacaactgcc 28020

gtaatcaaca tagccactgc gttgagcaga agcggataca acattgctgt ggtagataca 28080

gatccacaaa tgagcctgac gaactggagc aaagcgggca aggcagcatt tgacgtattt 28140

acagctgcat ctgaaaaaga cgtctatgga atccgaaaag atctggcgga ctatgacttt 28200

gctattgtgg acggggcagg ttcgctctca gtaatcacct ccgcagccgt catggtaagc 28260

gatctggtaa ttatccctgt tacacccagc cccctggatt tctccgcagc aggaagcgtc 28320

gttactgttc tggaagcaca ggcttacagc cgcaaagttg aagcccgctt tctgatcacc 28380

cgtaagatag aaatggcaac catgctcaat gtgctgaaag aaagtatcaa agacactggt 28440

gttaaatctt tccgtacggc cattacacaa cgtcaggttt acgtgaaatc aattctggat 28500

ggtgacagcg tgtttgaatc cagtgatggc gcagcaaaag gtgaaataga aatccttaca 28560

aaagagatag ttagcacatt tgagtaatta ctcattcact catataatca aataagtata 28620

acaaccggag taaccttaat gtcacttgaa aaagcgcata cggcagtaaa aaaaatgacc 28680

tttggtgaaa acagagatct ggaacgagta gtaacagcac cagtatcatc tggaaaaatc 28740

aaacgtgtta acgtcaattt tgacgaagaa aaacacaccc ggtttaaggc tgcatgtgcc 28800

aaaaaaggta catcgatcac agatgtggtg aaccagcttg tagataaatg gctcaaagag 28860

aacgaataat atctgaggat atatcatgga taaaaacgtc gttgatgcgc tgaaaacgct 28920

attagaagcg ttaccggaag aggtggtaac agaagtcaca tcaaaactaa atccttcggc 28980

aagccatatt cctgaagaaa acagtaagca attgacagca aaagcaagac tactgaattt 29040

ccggctaacc gaagcctatg aagaaatcct ggaagtcgaa gctatcagaa caggccagag 29100

caaaactacc gttctaaagg cagcactggc gatgtacaac agccaggatg aaaacataaa 29160

gaatcactgg ctacttgagt ctgcaaaaat taactaattc aggatataga ttattagtag 29220

gagttcgggc aataatggat gataactcta cgtcagtaac cagcaaagaa gatgtgcagg 29280

atatttcttt aaaaaactgg ctaaagaata aaaaaaccat tgtattactt atatttactg 29340

cattaacaaa tgttggcaat gtcctttcga cgattgatat tatcacagat aaaaccagtt 29400

ctttttacac atggcttggg gaatcgaaaa aattcgaagg ccattggacc aacaatacag 29460

aaggattcat agacggcact cctgattttt tactcaagaa tgcaggcgat gtcctcataa 29520

aatttgacct gaacatcaaa ggtggcgaag tgaggggaga gcttcacacc gacgcacgac 29580

taaaaatgtg tgaaactata gaagaaaagt taaaaacttt gtgtacatta atagcctcac 29640

atccactaat gattgagggg gaaaaatcac cattttccaa tgaattcgat gcctatatta 29700

ctgagtatag aaatggagat aaaaaaatag tcgcaatatt aaatatgaaa attacagacg 29760

atgacaaaat gacaataaca aatacaatga gaacaccaga atcagcatta ttttcaacta 29820

aaatatatgc tataaaaata ataaccgaag aataacttaa taacaaaaaa ggtattaaag 29880

tgaatactgg aataacaatt gatttgacca acctttcaga agatgaattg cttgatttat 29940

attcaatgta taaaagtgca aacatagcac atcaactatg gtgcagacgc catgaaaata 30000

taccagagca tttttcgata atattcgtga cgcttttaga acgaataaaa agggttacag 30060

aaaagaactc agaaggggta aaaacaccag atgtagacct ggatgcatta attgacacca 30120

tttatattgg ttgtcgttca atgttctgtg aaaaccctgg cttaaaaaat aactacactc 30180

tgcaaaactg cctgaggaaa gccaattatc acaacgaagc tagagtgata gataatattt 30240

tacaggaaaa aaaattcaca gattccatca tgaaagatga gtcatttttt agtctggtga 30300

aattagtttc caataagtcc attgcacacc aggagagcct ttcaggaaaa aaacgggaaa 30360

agatagacta tcgatataaa ttcttaaatg acaattcaaa tatctgtgag tttcagtatt 30420

acatttttag atgtcaccgt atttatgaga atattgtgaa agaatatggg gatacattac 30480

tgaatgagct taaaataaaa aacaatgata tataaagaat gcccccatat gaatagaagg 30540

aagaatcata ctaataaatt ttccagctta tcgctggtaa gcaattctgc cagcgaatca 30600

catttcataa aatcaatatc catttcgtcc agaacatcac agtagctgac atcatcaaga 30660

ctaatgacaa tcacaggaac accctctttg agatcaaaca ctaagacagg taatgaaatg 30720

gagtttatct tttcaaaatc actaaaaaac tgttcagttc catattcact ggcaggaaaa 30780

agaatctcac ttaatctcac acaataaaaa acatgatagt gttctttaag gttatccttt 30840

aaaatattaa cagccttagt atatttttcg ttaataaact ctttgaccaa atatttcata 30900

aaactctcca acaagaaccg actgtaggtc atcgggcaaa cgttgcggaa tggcgtcaga 30960

gacgtcattt tgcggcgttt gccctatcct gcatcgcagt ggcatcattt ctggcttatc 31020

tcgctactgt tctcggtgct ttcgtccgca ggttcgggaa caagtaactt gtgccagggc 31080

atagttttgt tgagcagctc catcagaggc tcagccagca gtcccaaaat ccaggttgcc 31140

gtaatcaccg gtatagtgag attttgtggc gtccataact ggtgatcgag atgataccag 31200

gtgatgacac acaaaatacc acccagccag aacacagccc atttaaacgc accaagaatg 31260

gctaacacaa aaacaaagat aaaatgtaaa acactcgcgg caagaagacg aagataaaac 31320

catccccagt caagccactt aacaaaagtg ctccctgttt tcactgcaaa acgtttctta 31380

cgctccctgg cgatttcttt cctgaattta gcacgttctt ccccctgagg gaattgatag 31440

attttagcca tgagttacct catttcgatc tcaaaacgaa gtttatgcaa ttatcctctg 31500

aaaccgtatg gtattctgat atctaggcct tcactttctt tatctatttt ataagaaaac 31560

cttagctttg atgctcttct tgcgactctc tcaacgattt taacttcgag atgattaatc 31620

tctgtatatt gatttatttt ttcaatgttt ttttgcagaa aaactttatt aaagtcctta 31680

aaattctgat aaaggtaaac tttttcgcca ttatctattc tgaataattc aagttcatcc 31740

tttaatacat ctacttcaat gtcaaaaaat gtctttttac cactgctgta ttgtttcctg 31800

ataaactgat aaagcatatt tgaattttga tcagcaagtc ttacagacga tatcaaaacc 31860

tgagtagtgt aagaatcctt taacatcgtg atatatggct caatgacatg tgaaaaagca 31920

atatcaatat atccttcact ttcaacataa tcacaaaacg cagtcaggtt cataagcctg 31980

actgcatcag aaggtaattt acctctcttc ttcgtatgtc cagagatttg attgccctga 32040

gctgtgagaa gttgatttcg aggaatgcga ataacactgg cctgaagttc ttctgccccc 32100

tctttcaact gccggtaagc acctgttaca tcaatatcag cgataaatgc atattcccga 32160

gccgtaatac gaaaggttgc cccctcagat aactcttcac gtgaatcaat ctgcgccata 32220

gccataaaca aaattcttct ggcagacaaa ggcaacgatg agaatgtgct gttaatttca 32280

tttctgtggc gaatttttgt tttctttgta acgctcagta agttcataca ttacctgtgg 32340

tttttaagtt ctaatggtga aatcataacc acaaaggcgg aaatatgtaa agtggtttat 32400

ccaccttatg tatttccacc aaaacacgga tcacatgaaa gaatttttat cattaatatt 32460

caatatgata gaaatgcgca ggtatccacc ttttaacgtc atacgttaga ttagagcact 32520

ataaagaact aaagaaatga agatacaaag atacgaatat ctgaataaaa acaggaaaat 32580

gcgtacccat ccacctttca gtgcgtaccc atccaccttt cagtgcgcag ccatccacct 32640

ttcatgaaaa ttacagtgcg tacccatcca cctttcagtg cgtacccatc cacctttcat 32700

tttgaggcga caaaaaacaa cacctatctt aatgaaatta ttgataaaaa aactggcaca 32760

atgtttgcag aataagttaa agtaagttaa agatcttaaa gtagatctac cgatctacat 32820

ttaagatctt aaaaacaaga agtttaataa gcaaaaaaac acaaaaaatc tgtggataac 32880

tttgctcaaa acaaaaaatt cacactacct gtggataact ttgcgtaaac ccggaggaca 32940

gatcactctg 32950

<210>3

<211>837

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>3

atgaacttac tgagcgttac aaagaaaaca aaaattcgcc acagaaatga aattaacagc 60

acattctcat cgttgccttt gtctgccaga agaattttgt ttatggctat ggcgcagatt 120

gattcacgtg aagagttatc tgagggggca acctttcgta ttacggctcg ggaatatgca 180

tttatcgctg atattgatgt aacaggtgct taccggcagt tgaaagaggg ggcagaagaa 240

cttcaggcca gtgttattcg cattcctcga aatcaacttc tcacagctca gggcaatcaa 300

atctctggac atacgaagaa gagaggtaaa ttaccttctg atgcagtcag gcttatgaac 360

ctgactgcgt tttgtgatta tgttgaaagt gaaggatata ttgatattgc tttttcacat 420

gtcattgagc catatatcac gatgttaaag gattcttaca ctactcaggt tttgatatcg 480

tctgtaagac ttgctgatca aaattcaaat atgctttatc agtttatcag gaaacaatac 540

agcagtggta aaaagacatt ttttgacatt gaagtagatg tattaaagga tgaacttgaa 600

ttattcagaa tagataatgg cgaaaaagtt tacctttatc agaattttaa ggactttaat 660

aaagtttttc tgcaaaaaaa cattgaaaaa ataaatcaat atacagagat taatcatctc 720

gaagttaaaa tcgttgagag agtcgcaaga agagcatcaa agctaaggtt ttcttataaa 780

atagataaag aaagtgaagg cctagatatc agaataccat acggtttcag aggataa 837

<210>4

<211>231

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>4

atgtcacttg aaaaagcgca tacggcagta aaaaaaatga cctttggtga aaacagagat 60

ctggaacgag tagtaacagc accagtatca tctggaaaaa tcaaacgtgt taacgtcaat 120

tttgacgaag aaaaacacac ccggtttaag gctgcatgtg ccaaaaaagg tacatcgatc 180

acagatgtgg tgaaccagct tgtagataaa tggctcaaag agaacgaata a 231

<210>5

<211>621

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>5

atgaaagtaa tctcatttct gaatccgaaa gggggttcag gtaaaacaac tgccgtaatc 60

aacatagcca ctgcgttgag cagaagcgga tacaacattg ctgtggtaga tacagatcca 120

caaatgagcc tgacgaactg gagcaaagcg ggcaaggcag catttgacgt atttacagct 180

gcatctgaaa aagacgtcta tggaatccga aaagatctgg cggactatga ctttgctatt 240

gtggacgggg caggttcgct ctcagtaatc acctccgcag ccgtcatggt aagcgatctg 300

gtaattatcc ctgttacacc cagccccctg gatttctccg cagcaggaag cgtcgttact 360

gttctggaag cacaggctta cagccgcaaa gttgaagccc gctttctgat cacccgtaag 420

atagaaatgg caaccatgct caatgtgctg aaagaaagta tcaaagacac tggtgttaaa 480

tctttccgta cggccattac acaacgtcag gtttacgtga aatcaattct ggatggtgac 540

agcgtgtttg aatccagtga tggcgcagca aaaggtgaaa tagaaatcct tacaaaagag 600

atagttagca catttgagta a 621

<210>6

<211>252

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>6

atgagctatc aaattctgac aaccacagcg gccagtatta ctgacctgaa aaaaaatcct 60

atgggaaccg tagctgaagg tgaaggggac gctgttgcga tcctgaaccg aaacgaaccg 120

gcgttctatt gcgttccacc aaaactttac gcctactatc gggaactcgc tgaagatgct 180

gagttaaacg ctgttgctga tgagcgcatg aaaaacccgg aaattgtgaa ggttaacctg 240

gatgacctat ga 252

<210>7

<211>282

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>7

atgacctatg aactggcttt tgaccgtaga gcactgaagg aatggcagaa actcggccac 60

accatccgtg aacaattcaa aaagaaactg gcagaacggc tggaaaatcc acgcgtaccc 120

gcagcccggt tacatggtca tgctgatcgc tataaaatca aacttcgtgc atctggctac 180

agacttgtat atcaagtcat tgatgagaaa gtcgttttac ttgttatttc cgttggaaga 240

agggaaagca gcgaagtcta tcagatcgca gatttgcgct aa 282

<210>8

<211>648

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>8

atgttatcta cttctacttt tcttgcgctt gccatgcaat gcgctgccag cgttcatccc 60

gacacaacgc acgaagtcgc cagggttgaa tcaggtttta acccatatgc gattgcggaa 120

ataataccaa aggttaaacg taaaactggt gataaaggcg tagtatctta ctttcctgaa 180

tcaaaggagg cagcacttaa gatcgttaaa aacattgaat tacggaatca tcgttactct 240

gtaggactta tgcaaataac gagtaccaat tttgcaaagt tcggtacaac agcagagaaa 300

atgtttgatc catgcgaaaa tcttaaggta tcagaaaaaa tactggttga ctgttataaa 360

cgaggtggcg acttagtgcg tgggctgagt tgctattatt ctggcaatca agaaacagga 420

gtaaagccag aacctgaatt taataataca agttatgtac aacgtatagg atttagccct 480

cctgataata aaaaaagttt tattgttccc tctgtaaagg aaatgattaa aaaggagaat 540

aagacgacta tcacacctga agaaatcatt atatatcctc aatacgccat gcgtggcact 600

gtatcaaatg aaaaggaaac aaaagatgtt gaaattaaat ctgaataa 648

<210>9

<211>297

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>9

atgttgaaat taaatctgaa taaacgttat ttaacgcttt ctctatttat ggctgcgttg 60

atgctttgtg ttgcagaacc tgcttttgcg gatgatgtgt ctacgaagac aactggtttt 120

ttacagaaaa taattgattt tttaacggat attcgtaaac ctgcaattac aattattgct 180

cttgttattg ggtatattgc gatattttct cgtcaacata cctcatggat agtcccactt 240

gttatcggaa ttatcatctt tattgttgca ccatatattc ctgactggct tgcgtaa 297

<210>10

<211>2754

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>10

atgagtactc tttttaaagg tcttacgcgc cctgctttaa taagggggct gggcgttccg 60

ctctacccct ttcttggaat gtgcattatt tgtgttctgc ttggtgtctg gattcatgag 120

gctatgtatg cccttattct tcctggctgg tatgccatca ggcgtgtaac acagtttgat 180

gaacgctttt ttgaccttct gtatctgaga actcttgtca aagggcatcc tttatcaaac 240

aagcgattca gcgcagtcca ttatgcgggg agccagtaca atgaagttga tatttcaaaa 300

gtggataact ttatgaagct gaaagaccag tcttctgttg aagagttaat tccgtactct 360

tcacatatca ctgataatat tatcgttaca aaaaaccggg atttgctggc aacctggcag 420

atagacggtg cttactttga gtgtgttgat tctgaagatt tgtcaattct gacagatcag 480

cttaatacgc ttatacgtag ctttgaaggg aaatttgtta cgctttatcc tcatcgtatc 540

aggtgtaaaa agggcgtcag accagtattt aacagtaaaa ttccttttgt gaacagagta 600

atgaatgatt attacgagtc attccctcag tctgaatttt tcgagaataa attatttctg 660

acgatttgtt ttaaaccttt tactacggaa gataaagtaa cacatttctt ttcacgcagt 720

aaaaaacaaa aagatatctt taaagagcct gttaatgaaa tgaatgaaat ttgcgacagg 780

ttgaatacct atctgtcccg ttttcattcc cgacgtcttg ggctttatga agatcatggg 840

gttgtttatt cagatcagtt atctctgttc cagtatctgc tttctggtcg atggcaaaag 900

gtcagggtta gcagtagtcc gttttataca tatctgggag gaaaagacct gttctttggt 960

aatgatgccg gacaaattac cgcgtcagac catgcccggt attttcgttg catagagatt 1020

aaggattatt ttcaggagac ggatgccggt attctggatg ctctgatgta tctccccgtt 1080

gagtatgtcg tgacatcgtc ctttactgcg atggataagc agtcagcgat taaggcgctg 1140

gatgatcaga tcgataagct ggaaatgaca gatgatgctg ccaaatcttt gctggcagat 1200

ctgaaagtcg gactggatat ggtttccagt ggatatattt ctttcggaaa atcgcatcag 1260

accctggttg tctttgcgga ttcaccggag cggctggtga aagacaccaa tatcgtgact 1320

tccactctgg aagatttggg gctgattgtc acttattcaa cactgagtct tggcgcagct 1380

tattttgctc agctaccagg aaattatacg cttcgccctc gtctgagtac cctcagtagt 1440

cttaattttg ccgaaatgga aagttttcat aatttctttt caggaaaaga aaaaggaaat 1500

acctgggggg aaaaactgat tactcttcgg gggtcaggta atgatatcta ccatctgaat 1560

taccatatga cgactgaaca tcagaatttc ttcggtaaga acccgacgct ggggcatacc 1620

gaaattctcg gtacgtctaa cgtgggtaaa accgtattac tgatgacaaa agcatttgcc 1680

gcccagcagt tcggtacgcc ggaatcattc cctgcaaaca gaaaactgaa aaaactgacc 1740

acggtttttt ttgataaaga ccgggcaggt gaagtcggta tacgggcaat ggggggatct 1800

tattaccggg tgaaggaggg agagccgaca ggctggaatc ccgccgcact gccgccaaca 1860

aagcgtaata tcgcttttat gaaggacctg gtgaggctgc tttgtactct caacagtgag 1920

ccgctcgatg attaccagaa cagcctgatt tcagatgcgg ttgaacgtct tatgcaacgg 1980

tcagatcgct cttatcctgt cagtaaacta cggcctctta tccaggagcc ggatgatact 2040

gaaaccaaac gtcatggact taaagcccgt cttaagccgt ggacgcaggg ggaagagttt 2100

ggctgggtgt tcgacaatcg ggaagacacg tttgatgtcg ataacctgga tgttttcggt 2160

attgatggaa cggagttcct ggataataag gtgctggcca gtgctgcttc attctatctc 2220

atctatcggg tcaccatgct ggccgatggt cgcaggcttc ttatctacat ggatgagttc 2280

tggcaatgga tcaataacga agcgttcagg gactttgttt acaacaagct gaaaaccgga 2340

cgtaaactcg atatggtgct tgtcgtagcc acacagtcac cggatgaact gattaaatca 2400

cccattgcgg cagcggttcg tgagcaatgc gccactcata tctatctggc aaacccgaaa 2460

gccaaacgta gtgaatatgt tgatggtttg caggtcaggg agctttattt tgacaaaatt 2520

aaagctatcg atccgctgtc ccgccagttc cttgttgtta agaacccaca gaggaaaggt 2580

gaaagtgatg attttgctgc ttttgccaga ctggagctgg gaaaagcagc gtattactta 2640

ccggttctca gtgcatcaaa accccagtta gaactgttcg atgaaatctg gaaagaagga 2700

atgaagccgg aagagtggct tgatacctat ctggaacagg cgaacctgat ttga 2754

<210>11

<211>759

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>11

atgaaaaagc aaattatggc ggcattcgtc gcttcactga ttgttatttc cggcgctcag 60

gcagggatac ctgttgccat cgacgccaac cctgaatggg cgattgaagc cggacgatgg 120

acagaacgcc ttaagcaatg ggcggaaacg gtaaaacatt acgaaaatca gataaatgcg 180

tacaaacagg agctgctgtc aaaaacgggt atccgtgatg tgcagggact ggtgcagtcc 240

gcacagtcag tgagtcagga actgatgcag atttatgatc aggggaatgc ttttattgac 300

gattacatta aaaatcctga aggggcgtta tcggaacagg ccaaatcgtt attgtcagat 360

tacaaagtaa cggatacctg ccagaacctg ggatattccg gcgacctggt acggggatgt 420

gaagcgacgt tcctttctca actggcaagc gtggaatacg gtaacaagct ggagagcaag 480

cttcgtcagg acaaccagac gatgaaagac cttattgatc aagtcaaaaa tgcgaaggat 540

acgaaggcca cgcaggatgc aacaaacgct gttgcacttg aacaactgaa gttcgagaag 600

ctcaaatttc agtatcaaat gtatcgcgat aagcagcgag atcttgcaga atataaagag 660

aagatggctc aggcagcttt cagaaaacag caacgtgaag ccgtgccacc ttcttacaga 720

aaagcttata tggcaatgaa atcatatgag gatgattaa 759

<210>12

<211>1134

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>12

atggggattg tcactggtat accggatggt actgatttat caggtaatgt aacctctccc 60

gcaaatgcgg gaggtgtttc tggatttgat tctgattttt ttcagacaac tcatgaagtt 120

atatttaata ttctcaacaa gagtatatct ggaaaattaa gtgaatattc agatgtggct 180

tatactcttg gtaaatatgg agtttctctg tatgttttat ggtatgcttt tactgtatta 240

gcaaggaaac aacagacacc tgtacctgat tttatctgga atatctgtag gttttacata 300

atattgcttt ttgttaagaa tacaggggga atacttacat cagcaacaga tgctattgat 360

ggattgaaaa atacattggc agggggagat ccgtgggtat ggatggatca gttatgggtg 420

aaggttatac aagttgcaac tcttattttt gataaagata catctactgt gcctgttgcc 480

ggtgggattg gtgctttatt aacttatgtc ggtggtgttt tggcattatt gctttgttct 540

atagtatttg catctgctga attaacatta ctattacttt ctgtcactgc gccaatattt 600

atcatgtgtc tgatgtttgg tttacttcgg caaatgttta atagctggct acagcttaat 660

tttagctcgt tactggtttt tctatttgca gcattagcac taagagctgg gacatggcaa 720

ttaaacatgg cattaagtac gtctattgct acagcatcag aaaacaatct tcttcaaacg 780

ggagtaactt cattagctgc tggcattttc atggcctgga ttatctggca ggcgaaaagt 840

tatgcttcac agattgcagg tgtgggtgtt gaaggtgcca tgcagggcgc agccgctatg 900

gggattggtg ctggcgtttt cggtgcatcc cgtatggcgc gtggcgcact tggtatgggc 960

agaaatgccg gtattggtgc atggaaaggt ctgcgtcgtc aggaagacgg gtttggtcag 1020

tctccgggaa taacgggtaa gaccgctaac ctggccggac agggcgttaa tattggtgcc 1080

aaaaagcttc gtcaggcagc tattgagaga gcaaagaaaa tgtatggtgg gtaa 1134

<210>13

<211>129

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>13

atgaaactac ttattgtggc tttcgtgacg ctttgtctcg ctggttgtca ggcgtcacat 60

aaactaccgc ccgtttccgg gaaaagcgaa cctgttaatt ctgctgaggt aatgcaaaat 120

ggaatttaa 129

<210>14

<211>714

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>14

atggaattta aacttcccgg atttaaaaat aaaaaagacg ttactgactc atcagtttca 60

tttgaagaaa aaaacattgc actacaggag agaatgaatc gtatttataa attcggtggt 120

atcggaggca tgttaattgg tgggctgtct ttacttgcat taaatgcagc attaccactg 180

aaaacaacag ttgttgatgc ctaccttatc gataaggtta caggtgtggc tgaacgtctg 240

acttctgtta aaaaagaaaa tctttctgaa aacgaagcca ttgcccgata ttttatcacc 300

cagtatataa aacatcgtga aggttataat tttttcagtc tccagcatga ttatgattat 360

gtaatggctt acagcgcgga gaatgtcgcg gcagattata acgcattatt taacagtgaa 420

caggcaccaa aacttgttta taacaaagca gaaaaaacgg caatggttca ggataatcca 480

tctgtcataa tttcaccttc gtcacgggca gatgataaag atatcggtgc gtatattcgt 540

tttcgtctga ccatcaggga tgttgctacc ggacaaaccc gccaggagtt ctggaatgtt 600

cgcctgactt atcgtatcga accgcaggtt gaaatggtgt caggggaacg taataacaat 660

cctcttaaat tcgttgtaac aagctacgtt cgcgataaag aagccagagg ttaa 714

<210>15

<211>930

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>15

atgaaaatga ataaaggagc gttaattatg gcgcttctga tggcggcgca cgtctgtcat 60

gcagctgttc ttccttcagg cagtcgcttt gacccacgca atcagatagt cagttataac 120

cccaataata ccaccataat taacagtgcc gttggataca ccaccacact ggtatttgat 180

gaagatgaaa cagttatcag tgccagaact ggttttccgc agggatgggc ggttaataaa 240

gaagataacc tggtatacct ggaagttcgt cctgttaaac agactgttca gaaaaataat 300

atggatgaaa acggtaatac ctcttctgaa tccgtcagtg ttgctcttga cccggaaaat 360

gagcttgaac gctggcgaac gaatttgttt gttcgcacca cgaagcgtaa ttacagcatg 420

gagctgaacg cccggacgtt ccggcagccg gagaaaattg cgtttgtggt gaattaccag 480

tatccgcagg aacgccggaa ggaacaggcc gaaattgaga agaaacgcac agaggctctt 540

gccagacgcc aggaggagca ggcaatcaac cgttccctgg aaaatgcgaa atcgccccgt 600

aactggcagt actggaagcg ggttgctgaa ggcagccagg atatcagccc tgattatgca 660

tatgacgatg gccgttatac ctggttcggc ttcagtccgt taaagaaaat tcccagcgtc 720

tttgtgatga acggtatgca ggagactctt accaatcctg tgattaaaca gagcgggagt 780

tttacggctg ttggcgtacc ggttgataag cgttttgttt tacgtcttgg tgagcaggtg 840

gtggggattg agaaccaggg cttcggaaaa gtacgtttac cagccggaga tacggtatcc 900

ccggatgtta agaaagaggt gatccagtga 930

<210>16

<211>1206

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>16

gtgactgaac aggaaaataa aatcccgact gcaaccgaaa ttgaacagca gctacgggaa 60

cgcagacaga aagaactgga acaggccggg aagactccgg aagaagagcc tggcaagcca 120

gcattgcagc ttggtattga aaaacttaaa aagtcacgta aagggatgat tatcctcgtc 180

gtgggttttc ttctgcttgc tgccggtgtt tctgtttatt atatcccgtc cattatccgt 240

tctgtgtcgt caggggatga gaaacccgca agtcagccgc ttgcaaccgg aacggctaaa 300

cgtcagaccg gactgagcga agatatcgat ccttttaata ccgcacagaa aaaaacagag 360

aaaccagagg aagaaaaagt catttcttct gaaaagactg aaccgccgga aaataaacag 420

cagagcttca gccgtgcact tgacgtttct cttgatggca gccagacagg aaacagcagc 480

agttcagcgg gaacgtcagt ttcacatact gcggccagtg agccagaaag cgataaaaag 540

gatgaagcaa aagcaaccgc acagactaca gaatctgcgc cactggcgaa aataacgaaa 600

cttccatatg acccaaattt gtttatcccg gaagggacat caattccctg ttcactggac 660

aggcgttttg tttctgacct ggcggggaaa ctggaatgta cggtcaacag cgatatatac 720

agcgccagcg gtaatgtaaa acttatcgaa agaggaaccg ccgcaaaact gatgtataag 780

gccgggtctt taaatcatgg acaagggcgt gtgtttgtta tggcttacaa gctacgtacc 840

cgcagtaagc cttttattga tattcccctg gttgactcac aggcggctgg cgcgttaggc 900

gaagccggtg cttctgggtg gattgacact catttcagtg aacgttttct tggtgcaatg 960

atggtcggga tgataccgga tttaagtcag gccgccagtg gtattgcaca gaacaacagg 1020

gacagccaga ccgactatac ggcaaacagt cgccaggctt ttgctgatat agcacgcgaa 1080

gcattttcta atagtgtgaa tattccgcca acgctttata aaaatcaggg cgaaattatt 1140

actctgattg tcggtcagga tctggatttt tcaggcattt ataaactgaa aatgaaaggg 1200

ggttaa 1206

<210>17

<211>1032

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>17

gtgaataacg aaaacagaca tctgatttat gatgtggtca acgattattt ttatcactgg 60

ctgaatgaga ttgagggtgt cacggaaatt gctgttaacc gaccaggaga aatatttata 120

aaggtcaggg gaaagtggca atggtatgaa caaaagatga gttacagtga ttgtctttct 180

tttgcatcca cactggccga ttttcatgac ggcggttctg tgactcctga atatcccctg 240

cgctctgcca cgcttccggg tggagaacgt gttcaggttg tgatcccacc ggcaactgaa 300

aaagacactg tttctataac aatccgtaag ccgtcaggta tttttatcag tcatgacaaa 360

tttataaaac agggatttta ttcacgcgtc agtggtttaa gtggtgactc ggttatggaa 420

gataatattt ctgctttaat cacttccgga tattttgatc gggttatacc tgaatcactg 480

cgtcagggga aaacgatagt tttctgtgga gggacgggtt caggtaaaac tacctttgca 540

aatgcctgtc tggaatatat accgcatcat ctgcggtgta tttctattga agatactgat 600

gaggcaaaat tcagattcca taaaaaccat gtaaaacttt actatccggc agagggtgag 660

agtaaggtta ttacctcagc gagtcttctg cgttcctgtt ttcgtatgaa tccggacagg 720

attctgatga cagaaatcag gggggctgag gcatgggatt ttctgaaagc atcgagttca 780

ggccatgcag gaaacattac caccgttcac gaaagtagtc ctgaatatgc tgtgcttggg 840

attgttcagc gatgttatat gaatcctgaa tgtcagaatc taccattcaa tgtcatttta 900

agacgtgtac tgagtaatat tgatattatc atgagtatta aataccttga tgatgaagat 960

tttcgtttcg cttccggtat ttattacaaa caacttcatt ttgatgacta tttcagaaaa 1020

ctgaaggagt ga 1032

<210>18

<211>615

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>18

atgacgtctc tgacgccatt ccgcaacgtt tgcccggtga cctacagtcg gttattgtcg 60

gagaatttta tgaaaaaagt tcaattcaga attgatgaaa atcagcataa tgatttgctg 120

gattgtctta aaactcttta tccagatgaa ccagctttaa cagtagctaa aggcatgaaa 180

cttttagcaa atgctttatt aaaaagtaaa gctggcagta aggacataaa tacgtttttt 240

gataataatg attttatcaa aacaacgatg tacttaacag gtaaacaaag ggctgatatt 300

gaaagagctg ctaatcgtca cggatggacg ttatcacgag aatgtcgtta ccgcatacag 360

acgacacttg aaaatgaact ggatttcttt gaccaggaac tgctgatgat gaatcgttgc 420

cgtaattcaa ttgataagat cggtcgtaat ttccattata tcattgttaa tgatcagacc 480

agggttcttg ataaagatgg tttctatcag gatgcggagc gtcttacaac agaaattttt 540

aatcttaaga atcagtttga gaattacatt atgttatgta aagggagaac tgtttcaaat 600

aaagtggaga tgtaa 615

<210>19

<211>1842

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>19

gtgattatgt ctttaaaact ccccgataaa ggccagtggg tttttatcgg tctggttatg 60

tgtctcgtga catattatac tggttctgtt gctgtttact tcctgaacgg aaaaacgccg 120

ctttatatat ggaaaaattt tgattccatg ctcctgtggc gaataataac agagagtaat 180

atacggacag atatcaggtt aaccgctatc ccctctcttt tatcaggtat ggtttcgtct 240

ctcatcgtgc ctgtttttat tatctggcaa ctgaataaaa cggctgttgc tctttatggt 300

gacgcgaagt ttgccagtga taatgattta aggaaatcga aacttctgaa atgggagaaa 360

gaaaacgata ctgatattct cgtcggagca tataaaggta aatacctgtg gtataccgca 420

ccagattttg tatcacttgg cgcaggaacc cgcgcaggta aaggtgccgc cattggtatc 480

cctaatcttc tggtcagaaa acactctctg attgcgttag atccaaaaca ggaattgtgg 540

aaaatcacca gtaaggtgcg tgaaatactg ctgggtaata aagtttatct gctcgaccct 600

ttcaacagta aaacacacca gtttaatccc cttttctata ttgatttaaa agcggagagt 660

ggggctaagg atctgcttaa actgattgaa attctgtttc cgtcttatgg catgacaggg 720

gcagaagcgc actttaataa tcttgcgggt caatactgga caggactggc taagttgctt 780

catttcttta ttaactatga gccgtcctgg cttaatgagt tcgggcttaa acccgttttc 840

tcaatcggtt ctgtcgtcga cttgtacagc aatattgacc gggaactgat actcagtaag 900

cgggaagaac tggagggaac aaacgggctt gatgaaaacg cgttgtatca tttgcgcgat 960

gccctgacca aaatcaggga atatcacgaa acggaagatg aacagcgttc aagcattgat 1020

ggttctttcc gtaagaaaat gagcctgttt tatctcccaa ccgttcgtaa atgtactgat 1080

ggtaatgatt tcgatctccg tcagttgcga cgggaagata tcactgttta tgtcggtgtt 1140

aatgcggaag atatatcact ggcttacgat tttctgaacc tgtttttcaa cttcgttgtt 1200

gaagtgacat tgcgtgaaaa tcctgatttt gatcccaccc tgaaacatga ctgcctgatg 1260

tttcttgatg agttcccttc gattggttat atgccaatta ttaaaaaggg atcagggtat 1320

attgcaggtt ttaaacttaa actgctgaca atttatcaga atatcagtca gctaaatgaa 1380

atctatggta ttgagggagc caaaacgctg atgagtgctc atccctgccg tattatctat 1440

gctgtcagcg aagaggatga tgccgcgaag atatcagaaa aacttgggta tattaccact 1500

acatcaaaga gcacaagcaa gaaccgggga cgatcaactt cacagggcga atcagaaagt 1560

gaagcccgaa gagcactggt gcttccacag gaactgggaa cgctggactt taaagaagag 1620

tttatcatcc tgaaggggga gaaccctgtt aaagcagaaa aggcacttta ttaccttgat 1680

ccgtatttta tggacaggtt aatgaaggtc agtcctaaac tggcatcatt gacgatgaaa 1740

ctaaataaga cgaaaaaaat atttggtgtg aaagggctta aatatccgtc aaaagaaaaa 1800

atgctctccg taggagagct ggagtctgag gttttgctat ga 1842

<210>20

<211>1167

<212>DNA

＜213〉Salmonella enteritidis (Salmonella enterica)

<220>

<223>

<400>20

atgggcgttt acgttgataa agaatatcgt gttaaacgaa agtcatcaga aaatggtcgt 60

aagtcagctt tcgctcacaa agtcaaaaat ggtggaaaga actatagccg caatgttcag 120

gaacgtatca accgcaaggg tgccagtaag gaggttgttg tcaaaatatc tggaggtgct 180

gttactcgtc aggggattcg gaacagtatt gattatatga gccgtgagtc agagctacca 240

gtgatgagtg aaagcggtcg ggtatggatg ggtgccgaaa ttctggaggc taaagagcac 300

atgatagatc gtgctaatga tcctcagcat gtgatgaatg ataaaggtaa agaaaataaa 360

aaaatcacac agaatattgt cttctcgcct ccagttttag cgaaagtaaa gcctgaagat 420

ttgttggagt ctgtcaggaa aacgatgcag aaaaaatatc ctaatcaccg ttttgttctt 480

ggataccact gtgacaagaa agaacatcct cacgttcatg ttgtttttcg tatccgagat 540

aatgacggta aacgcgctga tatcaggaaa aaagatttac gggaaattcg tacaggtttt 600

tgtgaagagt tgaagttaaa aggttatgac gttaaagcga cccataagca acagcatgga 660

cttaatcagt ctgttaaaga tgcacataat acagcaccaa aaagacagaa aggtgtttat 720

gaggttgttg atattggcta tgaccattat cagaacgata aaacaaagtc taagcaacat 780

tttataaagc taaagactct taacaagggg gttgagaaaa catactgggg ggctgatttt 840

ggggacttat gttcgcggga aagtgttaaa gcaggtgatc ttgtcaggct gaagaaactt 900

ggtcagaaag aagtaaaaat cccggcgctc gataaaaacg gtgttcagca tggctggaaa 960

acggttcaca gaaatgagtg gcagttagaa aatctggggg ttaagggcgt agacagaaca 1020

ccttcagcca gcaaagagct ggtacttaac agccctgata tgctgctgaa gcaacaacag 1080

cgaatggcgc agtttacgca gcagaaagca tccacgttac agtcagaaca gaagctgaaa 1140

acagggatta agtttttggg cttataa 1167

<210>21

<211>5655

<212>DNA

＜213〉Photobacterium (Photobacterium)

<220>

<223>

<400>21

atgcctgcag atgaagcaag aggaggactc tctatgaaat ttggaaactt tttgcttaca 60

taccaacctc cccaattttc tcaaacagag gtaatgaaac gtttggttaa attaggtcgc 120

atctctgagg agtgtggttt tgataccgta tggttactgg agcatcattt cacggagttt 180

ggtttgcttg gtaaccctta tgtcgctgct gcatatttac ttggcgcgac taaaaaattg 240

aatgtaggaa ctgccgctat tgttcttccc acagcccatc cagtacgcca acttgaagat 300

gtgaatttat tggatcaaat gtcaaaagga cgatttcggt ttggtatttg ccgagggctt 360

tacaacaagg actttcgcgt attcggcaca gatatgaata acagtcgcgc cttagcggaa 420

tgctggtacg ggctgataaa gaatggcatg acagagggat atatggaagc tgataatgaa 480

catatcaagt tccataaggt aaaagtaaac cccgcggcgt atagcagagg tggcgcaccg 540

gtttatgtgg tggctgaatc agcttcgacg actgagtggg ctgctcaatt tggcctaccg 600

atgatattaa gttggattat aaatactaac gaaaagaaag cacaacttga gctttataat 660

gaagtggctc aagaatatgg gcacgatatt cataatatcg accattgctt atcatatata 720

acatctgtag atcatgactc aattaaagcg aaagagattt gccggaaatt tctggggcat 780

tggtatgatt cttatgtgaa tgctacgact atttttgatg attcagacca aacaagaggt 840

tatgatttca ataaagggca gtggcgtgac tttgtattaa aaggacataa agatactaat 900

cgccgtattg attacagtta cgaaatcaat cccgtgggaa cgccgcagga atgtattgac 960

ataattcaaa aagacattga tgctacagga atatcaaata tttgttgtgg atttgaagct 1020

aatggaacag tagacgaaat tattgcttcc atgaagctct tccagtctga tgtcatgcca 1080

tttcttaaag aaaaacaacg ttcgctatta tattaagtcg aggaggagaa agaaatgaaa 1140

tttggattgt tcttccttaa cttcatcaat tcaacaactg ttcaagaaca aagtatagtt 1200

cgcatgcagg aaataacgga gtatgttgat aagttgaatt ttgaacagat tttagtgtat 1260

gaaaatcatt tttcagataa tggtgttgtc ggcgctcctc tgactgtttc tggttttctg 1320

ctcggtttaa cagagaaaat taaaattggt tcattaaatc acatcattac aactcatcat 1380

cctgtcgcca tagcggagga agcttgctta ttggatcagt taagtgaagg gagatttatt 1440

ttagggttta gtgattgcga aaaaaaagat gaaatgcatt tttttaatcg cccggttgaa 1500

tatcaacagc aactatttga agagtgttat gaaatcatta acgatgcttt aacaacaggc 1560

tattgtaatc cagataacga tttttatagc ttccctaaaa tatctgtaaa tccccatgct 1620

tatacgccag gcggacctcg gaaatatgta acagcaacca gtcatcatat tgttgagtgg 1680

gcggccaaaa aaggtattcc tctcatcttt aagtgggatg attctaatga tgttagatat 1740

gaatatgctg aaagatataa agccgttgcg gataaatatg acgttgacct atcagagata 1800

gaccatcagt taatgatatt agttaactat aacgaagata gtaataaagc taaacaagag 1860

acgcgtgcat ttattagtga ttatgttctt gaaatgcacc ctaatgaaaa tttcgaaaat 1920

aaacttgaag aaataattgc agaaaacgct gtcggaaatt atacggagtg tataactgcg 1980

gctaagttgg caattgaaaa gtgtggtgcg aaaagtgtat tgctgtcctt tgaaccaatg 2040

aatgatttga tgagccaaaa aaatgtaatc aatattgttg atgataatat taagaagtac 2100

cacatggaat atacctaagt cgaggaggat ggcaaatatg actaaaaaaa tttcattcat 2160

tattaacggc caggttgaaa tctttcccga aagtgatgat ttagtgcaat ccattaattt 2220

tggtgataat agtgtttacc tgccaatatt gaatgactct catgtaaaaa acattattga 2280

ttgtaatgga aataacgaat tacggttgca taacattgtc aattttctct atacggtagg 2340

gcaaagatgg aaaaatgaag aatactcaag acgcaggaca tacattcgtg acttaaaaaa 2400

atatatggga tattcagaag aaatggctaa gctagaggcc aattggatat ctatgatttt 2460

atgttctaaa ggcggccttt atgatgttgt agaaaatgaa cttggttctc gccatatcat 2520

ggatgaatgg ctacctcagg atgaaagtta tgttcgggct tttccgaaag gtaaatctgt 2580

acatctgttg gcaggtaatg ttccattatc tgggatcatg tctatattac gcgcaatttt 2640

aactaagaat cagtgtatta taaaaacatc gtcaaccgat ccttttaccg ctaatgcatt 2700

agcgttaagt tttattgatg tagaccctaa tcatccgata acgcgctctt tatctgttat 2760

atattggccc caccaaggtg atacatcact cgcaaaagaa attatgcgac atgcggatgt 2820

tattgtcgct tggggagggc cagatgcgat taattgggcg gtagagcatg cgccatctta 2880

tgctgatgtg attaaatttg gttctaaaaa gagtctttgc attatcgata atcctgttga 2940

tttgacgtcc gcagcgacag gtgcggctca tgatgtttgt ttttacgatc agcgagcttg 3000

tttttctgcc caaaacatat attacatggg aaatcattat gaggaattta agttagcgtt 3060

gatagaaaaa cttaatctat atgcgcatat attaccgaat gccaaaaaag attttgatga 3120

aaaggcggcc tattctttag ttcaaaaaga aagcttgttt gctggattaa aagtagaggt 3180

ggatattcat caacgttgga tgattattga gtcaaatgca ggtgtggaat ttaatcaacc 3240

acttggcaga tgtgtgtacc ttcatcacgt cgataatatt gagcaaatat tgccttatgt 3300

tcaaaaaaat aagacgcaaa ccatatctat ttttccttgg gagtcatcat ttaaatatcg 3360

agatgcgtta gcattaaaag gtgcggaaag gattgtagaa gcaggaatga ataacatatt 3420

tcgagttggt ggatctcatg acggaatgag accgttgcaa cgattagtga catatatttc 3480

tcatgaaagg ccatctaact atacggctaa ggatgttgcg gttgaaatag aacagactcg 3540

attcctggaa gaagataagt tccttgtatt tgtcccataa gtcgaggagg agtaaaagta 3600

tggaaaatga atcaaaatat aaaaccatcg accacgttat ttgtgttgaa ggaaataaaa 3660

aaattcatgt ttgggaaacg ctgccagaag aaaacagccc aaagagaaag aatgccatta 3720

ttattgcgtc tggttttgcc cgcaggatgg atcattttgc tggtctggcg gaatatttat 3780

cgcggaatgg atttcatgtg atccgctatg attcgcttca ccacgttgga ttgagttcag 3840

ggacaattga tgaatttaca atgtctatag gaaagcagag cttgttagca gtggttgatt 3900

ggttaactac acgaaaaata aataacttcg gtatgttggc ttcaagctta tctgcgcgga 3960

tagcttatgc aagcctatct gaaatcaatg cttcgttttt aatcaccgca gtcggtgttg 4020

ttaacttaag atattctctt gaaagagctt tagggtttga ttatctcagt ctacccatta 4080

atgaattgcc ggataatcta gattttgaag gccataaatt gggtgctgaa gtctttgcga 4140

gagattgtct tgattttggt tgggaagatt tagcttctac aattaataac atgatgtatc 4200

ttgatatacc gtttattgct tttactgcaa ataacgataa ttgggtcaag caagatgaag 4260

ttatcacatt gttatcaaat attcgtagta atcgatgcaa gatatattct ttgttaggaa 4320

gttcgcatga cttgagtgaa aatttagtgg tcctgcgcaa tttttatcaa tcggttacga 4380

aagccgctat cgcgatggat aatgatcatc tggatattga tgttgatatt actgaaccgt 4440

catttgaaca tttaactatt gcgacagtca atgaacgccg aatgagaatt gagattgaaa 4500

atcaagcaat ttctctgtct taagtcgagg aggaaaacag gtatgacttc atatgttgat 4560

aaacaagaaa ttacagcaag ctcagaaatt gatgatttga ttttttcgag cgatccatta 4620

gtgtggtctt acgacgagca ggaaaaaatc agaaagaaac ttgtgcttga tgcatttcgt 4680

aatcattata aacattgtcg agaatatcgt cactactgtc aggcacacaa agtagatgac 4740

aatattacgg aaattgatga catacctgta ttcccaacat cggtttttaa gtttactcgc 4800

ttattaactt ctcaggaaaa cgagattgaa agttggttta ccagtagcgg cacgaatggt 4860

ttaaaaagtc aggtggcgcg tgacagatta agtattgaga gactcttagg ctctgtgagt 4920

tatggcatga aatatgttgg tagttggttt gatcatcaaa tagaattagt caatttggga 4980

ccagatagat ttaatgctca taatatttgg tttaaatatg ttatgagttt ggtggaattg 5040

ttatatccta cgacatttac cgtaacagaa gaacgaatag attttgttaa aacattgaat 5100

agtcttgaac gaataaaaaa tcaagggaaa gatctttgtc ttattggttc gccatacttt 5160

atttatttac tctgccatta tatgaaagat aaaaaaatct cattttctgg agataaaagc 5220

ctttatatca taaccggagg cggctggaaa agttacgaaa aagaatctct gaaacgtgat 5280

gatttcaatc atcttttatt tgatactttc aatctcagtg atattagtca gatccgagat 5340

atatttaatc aagttgaact caacacttgt ttctttgagg atgaaatgca gcgtaaacat 5400

gttccgccgt gggtatatgc gcgagcgctt gatcctgaaa cgttgaaacc tgtacctgat 5460

ggaacgccgg ggttgatgag ttatatggat gcgtcagcaa ccagttatcc agcatttatt 5520

gttaccgatg atgtcgggat aattagcaga gaatatggta agtatcccgg cgtgctcgtt 5580

gaaattttac gtcgcgtcaa tacgaggacg cagaaagggt gtgctttaag cttaaccgaa 5640

gcgtttgata gttaa 5655

<210>22

<211>101

<212>DNA

＜213〉intestinal bacteria (Escherichia coli)

<220>

<223>

<400>22

aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 60

ccggctcgta tgttgtgtgg aattgtgagc ggataacaat t 101

Claims (2)

1. can stablize self luminous bacterial plasmid for one kind, this can be stablized self luminous bacterial plasmid and have nucleotide sequence as described in SEQID NO:1.

2. one kind prepares and can stablize luminous and have method as the bacterial plasmid of the described nucleotide sequence of SEQ ID NO:1, comprises the following step:

(a), will contain P from the bioluminescence gene luxABCDE access one of plasmid pXen-5 shortage promotor _LacZIn the plasmid pGEM 3-Zf+ of promotor, make bioluminescence gene luxABCDE can utilize P in plasmid pGEM 3-Zf+ _LacZPromotor starts transcribes;

(b), will contain in the plasmid pSE34 of gram-negative bacteria plasmid pBlueScript II KS (+/-) access as shown in SEQ ID NO:2 of at least one ColE1 replicon and at least one resistant gene;

(c), the plasmid that step (a) is constructed accesses in the constructed plasmid of step (b) to make the stable spontaneous luminescence plasmid of this gram-negative bacteria special use.

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