201132291 六、發明說明： 【發明所屬之技術領域】 本發明係關於免疫賦活劑、免疫賦活劑之製造方法、 含免疫賦活成分的食品組成物、及使用免疫賦活成分的免 疫賦活方法。 【先前技術】 ‘一般認為屬於蔥屬（leek)之植物為於營養學上含有許 多量之胡蘿蔔素（carotene)、維生素C '卸(potassium)等成 分的蔬菜。又，已知屬於蔥屬之植物有獨特的刺激臭味， 又含有成為辣味來源的蒜胺酸(alliin)等之S-烯丙基（烯基 )半胱胺酸亞颯(S-allyl(alkenyl)cysteine sulfoxide)類為其 特徵。由此等之特徵，一般認為屬於蔥屬之植物具有刺激 交感神經且提高維生素B1之吸收、促進消化液分泌的效 果。 有幾種屬於蔥屬之植物因其機能性而被利用於健康食 品等。作為利用於健康食品等的屬於蔥屬植物之機能性， 已知有經由來自洋蔥（onion)外皮之槲皮素（quercetin)的抗 - 氧化作用、經由大蒜（garlic)之蒜素(allicin)的滋養強壯作用 、經由發酵黑大蒜的抗氧化作用等。 屬於蔥屬之植物中，尤其廣泛利用於亞洲區的蔥（leek ，Allmm flstul〇sum)，係於日本之收穫量i年達到約％萬 順的主要作物。惠向來被認為有民間傳承的感冒等之預防 效果，、免疫賦活作用被期待。與蔥同樣屬於葱屬的洋葱 201132291 (:聰cepa)於全世界之1年生產量達_〇㈣，而容易 獲得又關於洋惠，並不知有如惠已知之與免疫賦活相 關的民間傳承。 專利文獻1〜4記載葱有免疫賦活作用，關於利用此等 的食品或飲料的技術已被記载於專利文獻丨〜4。又，關於 由蔥萃取的成分，已發表評價對流行性感w (influenza)有用 f生的調査（非專利文獻丨）。再者，非專利文獻2〜4已記 載來自洋惠之成分影響細胞激素㈣也㈣産生或影響 與此專有關聯的訊息傳導。 [先前技術文獻] [專利文獻] [專利文獻1]日本國公開專利公報「特開2〇〇M9〇251 號公報（2001年7月π日公開）」 [專利文獻2]曰本國公開專利公報「特開2〇〇1_19〇252 號公報（2001年7月17日公開）」 [專利文獻3]曰本國公開專利公報「特開2〇〇4_3575〇9 號公報（2004年12月24日公開）」 [專利文獻4]曰本國公開專利公報「特開2〇〇2_〇2〇3〇5 號公報（2〇〇2年1月23日公開）」 [非專利文獻] [非專利文獻1]高野美耶等人，2009年度曰本農藝化 學會關西•作國•西日本支部、日本營養•食糧學會九 州冲繩支部、日本食品科學工學會西日本支部、合同沖 繩大會要旨集，p72 201132291 [非專利文獻 2] Lin JY，Tang CY，Journal of Food Composition and Analysis, 21(1), p45-53(2008) [非專利文獻 3] Tang CH, et al·， Osteoporosis International, 20(1), p93-103(2009) [非專利文獻 4] Ban JO, et al., Archives of Pharmacal Research, 30(11), pl455-1463(2007) 【發明内容】 發明概要 發明欲解決的課題 然而，於專利文獻1〜4 ’並不清楚蔥之免疫賦活作用 之作用機制、有效成分、物性等’此外由記載的技術亦無 免疫賦活作用之評價。因此’依據專利文獻1〜4記載之技 術，實際上是否能獲得免疫賦活作用並不清楚，因為是否 經由蔥所含的任一成分獲得免疫職活作甩並不清楚，無法 將此成分更效率地取出來利用。 又，於非專利文獻1亦同樣地’因為蔥所含的任一成 分是否對流行性感冒有用炎不清楚’有必要從蔥全體取出 有用成分，而其他部分成為無用的。此外，非專利文獻2 〜4記載之技術亦利用萃取自洋蔥可食部位的成分，萃取 必要成分後之殘渣變成無用’會廢棄大量食用部分。因此 ，自蔥及洋蔥更有效率地取出成為目的之有用成分時，無 法不將食用部分變成無用來利用。 鑑於上述問題點，本發明之目的係提供更有效率自蔥 201132291 料惠取出具有免疫賦活作用的成分，且利用此成分的免 疫賦活劑、免疫賦活劑之製造方法、食品組成物 賦活方法。 用以解決課題之手段 為了解决上述課題’本發明相關的免疫賦活劑之特徵 為含有自葉身部、或洋t之莖葉部（含有除去可食部 鱗莖部）的葉㈣及葉身部的雜）萃取的免疫賦活成分 〇 ^本發明相關的免疫賦活劑之製造方法的特徵為包含自 蔥之葉身部、或洋葱之莖葉部萃取免疫賦活成分的萃^步 驟。 本發明相關的食品組成物之特徵為包含自蔥之葉身邛 、或洋蔥之莖葉部萃取後經乾燥的免疫賦活成分與該I 賦活成分以外之添加劑。 、 ^本發明相關的免疫賦活方法之特徵為包含投與自箴之 葉身部、或洋葱之莖葉部萃取的免疫職活成分的投與^ 發明效果 本發明相關的免疫賦活劑因含有萃取自蔥之葉身A 或洋蔥之莖葉部的免疫賦活成分，可提供含有有2 Η 蔥或洋蔥取出的免疫賦活成分之免疫賦活劑。’;； 【實施方式】 用以實施發明的形態 201132291 〔免疫賦活劑〕 …本發明相關的免疫賦活劑含有萃取自葱之葉身部、或 洋蔥之莖葉部（除去可食部（鱗莖部）之外包含葉鞠部及 葉身部的部位）的免疫賦活成分。 於本發明所使用的蔥係指屬於百合科（Liiiaceae)蔥屬 之植物，以Allium fistulosum<學名所表示者。於本發明 所使用的S可為根_、’亦可為料，其品種或栽培方法 、採集時期等亦未限定。 作為根深蔥’可舉例加贺群加賀系之蔥、加賀群下仁 田系之蔥、千住群黑柄系之蔥、千住合黑系之蔥、千住合 柄系之蔥、千住赤柄系之蔥、九條群越津系之蔥、晚柚性 之蔥、不抽苔性之蔥、赤蔥等。又，作為葉蔥，可舉例九 條群九條太系之蔥、九條群九條細系之蔥、九條群越孝系 之蔥、加贺群岩槻系之蔥、不抽苔性之蔥、胎生性之 (viviparous onions)等。再者，除上述者.以外’亦可適當使 用中國、蒙古等之外國原産之蔥。 蔥主要由綠色之葉身部、白色之葉勒部、及根部所構 成。根深蔥之場合，將作為食用的葉鞘部進行擦入土中， 以未照光的方式栽培，向來葉身部於收穫後被廢棄。於本 發明，尤其是使用蔥之葉身内部所含的免疫賦活成分。因 此，依據本發明，可有效利用通常被廢棄的根深蔥之葉身 部。 如後述之實施例所示’蔥之葉賴部中不太含有本發明 中的免疫賦活成分。本發明中的免疫賦活成分因為使用萃 201132291 取自惠之葉身部的免疫賦活成分，與使用全體惠的場合作 比較，免疫賦活成分之萃取後成為無用的部分少， == 免疫賦活成分。又’萃取自葱葉身部的免疫賦 活成刀，與卒取自葱之葉勒部的免疫賦活成分比較，因並 免疫賦活效果高，經由將其用於免疫賦活劑，可獲得更有 效果的免疫賦活效果。 於本發明所使用的洋惠，為屬於百合科蔥屬之植物， 係指以Amum cepa之學名表示者。本發明所使用的洋惠， 並未限定其品種或栽料法、採集轴# 黄洋葱、赤洋1、白洋蔥。 羋例 洋惠主要由葉身部、含鱗莖部（被食用的球狀之部位 ί的葉勒部、以及根部所構成，葉身部及葉稍部之鱗莖部 以外之。Ρ分（通稱：莖葉部）被廢棄。於本發明，尤其是 使用含於洋惠之莖葉内部的免疫賦活成分。如此，因自、通 常被廢棄的莖葉部萃取免疫賦活成分，不會使食用的鱗贫 β成為無用，可更有效率萃取免疫賦活成分。 再者’自洋蕙之莖葉部萃取的免疫賦活成分，如後述 ^ 〇所不於亦較自洋葱之鱗莖部萃取的免疫賦活成 的免疫賦活效果上’自葱之葉身部萃取的免疫賦 /成刀有極南的免疫賦活效果。因此，經由將其使用於免 疫賦活劑’可更有絲㈣得免賴活效果。 、免 ^於本發明，免疫賦活成分係萃取自蔥之葉身部、或洋 k莖葉部者。作為自惠之葉身部、或洋葱之莖葉部萃取 免疫賦活成分的方法’並未制限定，可舉例水萃取法、 201132291 熱水萃取法、加壓熱水萃取法、超臨界萃取法等。如於後 述之實施例所示，依據水萃取方法，因免疫賦活成分之產 率高，所得免疫賦活成分之物性亦優異而為特佳。尤其， 如後述之實施例所示，以水萃取方法所得的免疫賦活成分 之免疫賦活效果較以熱水萃取方法所得者比較，其效果非 常高。 水萃取方法為以水作為溶劑，於水中使可溶成分溶出 的方法。使用的水溫度只要低於70°C即可並未特別限定。 經由自蔥之葉身部、或洋蔥之莖葉部將免疫賦活成分以水 萃取方法萃取的場合，例如將蔥之葉身部、或洋蔥之莖葉 部於長度方向切斷，使水浸透切斷面後，經由以手拉緊切 斷面的方式施加外力，使免疫賦活成分溶出水中者為宜。 又，可適宜使用基於如此機制所構成的機器等。 經水萃取方法萃取的場合，含有所獲得的免疫賦活成 分的萃取物係葉身部組織等不要物質之含量少且為有效成 分的免疫賦活成分之含有率高。經水萃取方法萃取的免疫 賦活成分幾乎沒有蔥屬植物特有的刺激臭且為無臭、無色 透明之黏性物質。 熱水萃取方法係使用較水萃取方法中所使用的水之溫 度更高的熱水作為溶劑，使可溶成分於熱水溶出的方法。 使用的熱水溫度為70〜100°C，更佳為90〜100°C。又，其 萃取時間為10分鐘〜30分鐘者為較佳。自蔥之葉身部、 或洋蔥之莖葉部以熱水萃取方法萃取免疫賦活成分時，葉 身部或莖葉部之前處理並非特別需要，於上述條件下將葉 201132291 身部或莖葉部煎煮，回收此煮汁為宜。又，熱水萃取方法 之情形，與水萃取方法比較，因免疫賦活成分於組織中之 殘存量較多，故將萃取殘渣浸潰、榨汁等進一步進行萃取 處理者更佳。又，以熱水萃取方法萃取時，含有所得免疫 賦活成分的萃取物因含有許多葉身部之組織等，萃取物全 體之收量多，但為有效成分的免疫賦活成分之含有率則低 ，具有蔥屬植物特有的刺激臭味，且為茶色混濁的低黏度 物質。 又，免疫賦活成分自蔥之葉身部、或洋蔥之莖葉部萃 取後，亦可進一步乾燥。作為使萃取的免疫賦活成分乾燥 的方法，並未特別限定，例如可舉例將萃取物過濾後，以 冷康乾燥（freeze dry)、轉筒乾燥（drum dry)、喷霧乾燥(spray dry)等乾燥的方法等。 經水萃取方法使萃取物乾燥時，可獲得無臭且顆粒細 的白色粉末。另一方面，經熱水萃取方法使萃取物乾燥時 ，會獲得有些許刺激臭、顆粒粗之茶色粉末。經由使免疫 賦活成分乾燥，免疫賦活成分之使用及管理變容易。 本發明相關免疫賦活劑中所含的免疫賦活成分係含於 蔥之葉身部、或洋蔥之莖葉部，經活體内投與，會增加該 活體中的免疫能力者。免疫賦活成分係經由自蔥之葉身部 、或洋蔥之莖葉部萃取而被萃取。免疫賦活成分為水溶性 ，利用此性質，可以水、熱水等作為溶劑來萃取。又，經 萃取的免疫賦活成分有耐熱性。 自蔥之葉身部、或洋蔥之莖葉部萃取的免疫賦活成分 201132291 取方法而異，於以水萃取方法萃取的場合，所 二植物特有的刺激臭味，為無臭 物，屬植物特有的二:熱 水 等的不因含有^免疫航成分以外之葉身部組織 。、 f’經由水萃取方法之萃取物與其物性會不同 本發明相關的免疫賦活劑中所含有免疫賦活成分瘦由 促進細胞激素之生成、或促進㈣細胞之吞食作用，使活 體之免疫能力增強。即，免疫賦活成分係活性化活體内之 免疫機制，尤其相較於抗體生成更早階段之免疫初期反應 。又，於本說明書’所謂免疫賦活不僅係指將活體所具有 的免疫機能活性化的效果，亦包含基於高齡、幼齡、疾病 、壓力等之理由下活體之倾機能降低的情形中，使盆機 能回復到通常狀態為止之效果。 免疫賦活成分是否具有免疫賦活作用，可經由例如投 與本發明相關的免疫賦活劑活體中細胞激素之生成量、與 未投與該免疫賦活劑活體中細胞激素之生成量作比較來確 認。 本發明相關的免疫賦活劑於活體内經口或非經口投與 ，使免疫賦活成分於活體内吸收。於活體内，多形核白血 球(PMN)或巨噬細胞係負責於血流t運行而巡查全身的任 201132291 —本發明相_免疫賦活射所含的免㈣活成分，於 、’’JL 口 U if形’自4化皆以維持活性的狀態被吸收，經 血抓運#王身’由白血球促進細胞激素之生成，使巨嗤細 胞之吞食作用提升。 本發明相關的免疫贿H㈣活活性 ，例如以投 與免疫賦活成分的活體中之TNF (腫瘤壞死因子，_〇r necrosis factor ) -α的生成量(pg/mL)表示。本發明相關的免 疫賦活劑之免疫賦活成分經口投與為有效果者，係以後述 之實施例證明， 且因顯不即使於活體外亦有再現可能，便 利地於RAW264細胞、j 774>1細胞等巨嗤細胞系細胞株 之培養系統中添加評價對象（樣品）之際時，可以·_α 等之炎症性細胞激素的生成量作為指標。 具體而言，將上述細胞等以（1X105細胞/200μΐν孔） 调整後接種於96孔平盤’數小時的預培育後，以pBS( ) 等洗淨’添加溶解於添加FCS (胎牛血清）# RpMn64〇 、E,MEM #培養基之樣品（1〜l〇〇(WmL)，18小時後， 收取培養上清液。±清液巾TNF_af之炎症性細胞激素以 (酵素免疫疋量）、生物分析(bi〇assay)等測定。又， 實驗條件㈣使用的實驗設施或實聽件等，可適宜安排 又例如，將樣品之添加量規定為lmg/mLy時，亦可將 細胞激素料活性作為分類管理、規龍等之指標。又， ^規格値時’因依細胞之培養條件、狀態等，活性有波 動^凊形，设计陰性對照組(negative control)(僅培養基） 或陽陡對照組(P〇sitive control)(LPS (聚多糖）等），與此 12 201132291 等之活性作比較的相對値做處理者為較佳。又，未限定於 TNF α ’ 亦可以 il (介白素（interieukin) ) 、il-6、IL-12 MCP(單核球趨化性蛋白質，m〇n〇cyte chem〇tactic pr〇tein )-1等之其他炎症性細胞激素之生成量作為指標，未限定 於本實驗系統’亦可以即時^尺㈣丨time pcR)等之其他 實驗系統掌握細胞激素基因之表現量。 口 ^本發明相關的免疫賦活劑，係免疫賦活成分與藥學上 可备許的制混合，可作為錠劑、丸劑、顆粒劑、膠囊劑 、劑、_、料料經口投與，或亦可作為注射劑 枚，、舌下錠等靜脈内、皮下及肌肉内等非經口投與。 谷許的載體’可使用向來習知之各種有機或 墙：質’例如可舉例賦形劑、潤滑劑、結合劑、崩 ^二溶劑、溶解輔助劑、懸浮化劑、等張化劑、二 無=劑等。又，免疫賊活劑，視必要： d、抗氧化劑、荽名态丨 男I万腐 J者色劑、甘味劑等添加劑。 成分體内投與免疫賦活劑中的免疫職活 成為投與對象之活二:::重免：=輿量依據 蜱活效果的程度等可適 〃'的之免疫 投與坤Μ，以從a適、擇。又，即使於免疫職活劑之 選擇。本發明相=為目的之免疫賦活效果的方式可適宜 後與對象之體重= 购舌劑之投與量’例如’相對^ 例如可為至少i曰〜2 0日可為〇.3〜3呢’投與期間， 法體内之免疫機^ 發明相關的免疫賦活劑，對 1中相對初期階段之免疫反應起作用，故 13 201132291 於活體内，其免疫賦活效果於投盥開3 老化關的免疫賦活劑可預防基於因感染或壓力、 疾病荨活體之免疫能力降低所認為的各種疾病 用於提升初期癌細胞之排除、過、吏 p ^ ^ 曰月戏糖質之排除、自 老售廢物之排除等作用。更具體而言，本發明相關的务 疫賦活劑對哺乳動物（例如小鼠、貓、犬、牛、馬、羊、 山羊、家兎、人等）、鳥類、魚類、軟體動物（貝類等等 發揮免疫賦活作用，有用於各種細菌、真g、病毒感染症 等=治療或預防。例如，將本發__免疫賦活劑，經 由投與至具有腫瘤的哺乳動物，可發揮延長壽命效果的情 形0 作為可使用本發明相關的免疫賦活劑的對象疾病，可 舉例各種白血病、惡性淋巴瘤、骨肉瘤、惡性黑色素瘤、 惡性絨毛上皮、肌肉瘤、卵巢癌、子宮癌、前列腺癌、胰 癌、胃及腸等之消化器官癌、肺癌、食道癌、頭頸部腫瘤 、腦腫瘤、肝癌等之病毒性腫瘤等，但未限定於此等。於 細菌或病毒感染之前預先攝取本發明相關的免疫賦活劑時 ，可獲得所謂難感染，進而即使感染亦容易治療的預防作 用。因此’亦可將本發明相關的免疫賦活劑作為健康食品 或醫藥品’讓活體每日攝取。又，本發明相關的免疫賦活 劑’亦可配合於鳥類、魚類、軟體動物（貝類等）等之養 殖用飼料使用。 本發明相關的免疫賦活劑因含有萃取自蔥之葉身部、 或洋蔥之莖葉部的免疫賦活成分，較含有萃取自蔥或洋蔥 14 201132291 之，、他#分成分的情形具有更高的免疫賦活作用。又，與 自蕙或洋全體萃取免疫賦活成分的情形比較，萃取後成 為無用的部分少，可更有效率萃取免疫賦活成分。尤其， 使用根深惠4 ’或使用洋葱時，因可回收向來未被食用而 被廢棄的部位，可謀求廢棄成本之減低及資源之有效利用 0 現在，尤其於根深蔥大規模生産地，以自動蔥收穫機 收穫蔥，經由自動根切機、自動剝皮機、自動葉切機等作 處，而規格化送出。即使未導入如此系統的産地，葉身部 於農場或收集場被切取而廢棄。大量產生不要的葉身部， 此廢棄要費工，而成為問題。 依據本發明，因利用如此通常被廢棄的部位，可促進 未利用資源的利用。又，因為自惠之葉身部、或洋葱之莖 葉部之有效成分之萃取方法亦利用既存之施設而為對應可 月t*，産業化亦容易。因此，亦連結廢棄物之減量化、回收 之推進、及農家副產品産業化。又，所得免疫賦活成分可 利用於健康食品産業，由於各種疾病預防亦有貢獻的有用BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an immunostimulating agent, a method for producing an immunostimulating agent, a food composition containing an immunostimulating component, and an immunostimulating method using an immunostimulating component. [Prior Art] ‘A plant which is generally considered to belong to the genus leek is a vegetable which contains a large amount of carotenoids, vitamin C 'potassium and the like. Further, it is known that a plant belonging to the genus Allium has a unique pungent odor, and also contains S-allyl (alkenyl) cysteine sulphate (S-allyl) which is a source of spicy taste such as alliin. The (alkenyl)cysteine sulfoxide) class is characterized. According to such characteristics, plants belonging to the genus Allium are generally considered to have an effect of stimulating sympathetic nerves and enhancing absorption of vitamin B1 and promoting secretion of digestive juice. Several plants belonging to the genus Allium are used for healthy foods due to their functional nature. As a function of allium plants used for health foods and the like, it is known that via the anti-oxidation action of quercetin from the onion skin, garlic (allicin) Nourish and strengthen the body, through the antioxidant effect of fermented black garlic. Among the plants belonging to the genus Allium, it is especially widely used in the Asian region (leek, Allmm flstul〇sum), which is the main crop in Japan with a harvest of about 10,000%. We are expected to have a preventive effect such as a cold that is inherited by the people. The onion that belongs to the allium with onion 201132291 (: Cong cepa) has a production capacity of _〇(4) in the world for one year, and it is easy to obtain and about Yanghui. I don’t know that it is known as the folk heritage related to immune alive. Patent Documents 1 to 4 disclose that the onion has an immunostimulating action, and a technique for using such food or beverage has been described in Patent Documents 丨 to 4. In addition, the investigation of the ingredients extracted from the onion has been published for the evaluation of the use of the popular sex (influenza) (Non-patent literature). Further, Non-Patent Documents 2 to 4 have recorded that the components from Yanghui affect the cytokines (4) and (4) generate or influence the signal transmission associated with this. [PRIOR ART DOCUMENT] [Patent Document 1] Japanese Laid-Open Patent Publication No. JP-A No. H9-251 (published on July π, 2001) [Patent Document 2] "Special Publication No. 2〇〇19_252 (published on July 17, 2001)" [Patent Document 3] Japanese Patent Publication No. 2〇〇4_3575〇9 (publication on December 24, 2004) [Patent Document 4] 曰 National Patent Publication No. 2〇〇2_〇2〇3〇5 (publication on January 23, 2002) [Non-patent literature] [Non-patent literature] 1] Takano Mie and others, 2009 Sakamoto Agrochemical Association Kansai • Zuoguo • West Japan Branch, Japan Nutrition and Food Research Society Kyushu Okinawa Branch, Japan Food Science and Technology Society West Japan Branch, Contract Okinawa Assembly Essentials, p72 201132291 [Non-patent Literature 2] Lin JY, Tang CY, Journal of Food Composition and Analysis, 21(1), p45-53 (2008) [Non-Patent Document 3] Tang CH, et al., Osteoporosis International, 20(1), p93- 103(2009) [Non-Patent Document 4] Ban JO, et al., Archives of Pharmacal Resea Rch, 30 (11), pl455-1463 (2007) SUMMARY OF THE INVENTION PROBLEMS TO BE SOLVED BY THE INVENTION However, Patent Documents 1 to 4' do not understand the mechanism of action, active ingredients, physical properties, etc. of the immune activation action of onion. In addition, there is no evaluation of the immunostimulating effect by the described technique. Therefore, according to the techniques described in Patent Documents 1 to 4, it is not clear whether or not the immunostimulating effect can be obtained, because it is not clear whether or not the immune activity is obtained by any of the components contained in the onion, and it is not possible to make the component more efficient. Take it out and use it. Further, in Non-Patent Document 1, the same is true because it is not clear whether any component contained in the onion is useful for influenza. It is necessary to take out the useful component from the whole onion, and the other portions become useless. Further, in the techniques described in Non-Patent Documents 2 to 4, the components extracted from the edible portion of the onion are used, and the residue after extracting the necessary components becomes useless, and a large amount of edible portion is discarded. Therefore, when the onion and the onion are more efficiently taken out as a useful component for the purpose, the edible portion cannot be used without being used. In view of the above problems, an object of the present invention is to provide an immunostimulating agent, an immunostimulating agent manufacturing method, and a food composition activating method which are more effective in extracting an immunostimulating component from the onion 201132291. Means for Solving the Problem In order to solve the above-mentioned problems, the immunostimulating agent according to the present invention is characterized in that it contains a leaf (four) and a leaf body portion from the leaf body portion or the stem and leaf portion of the ocean t (containing the bulb portion of the edible portion). The immunostimulating component extracted by the present invention is characterized in that the method for producing an immunostimulating agent according to the present invention comprises a step of extracting an immunostimulating component from the leaf body portion of the onion or the stem and leaf portion of the onion. The food composition according to the present invention is characterized by comprising an additive other than the I-activating component which is dried after extraction from the leaf body of the onion or the stem and leaf of the onion. The immunostimulating method according to the present invention is characterized in that it comprises administration of an immunologically active component which is extracted from the leaf body of the scorpion or the stem and leaf of the onion. The effect of the invention is related to the immunostimulating agent of the present invention. The immune revitalizing ingredient of the leaf body of the onion leaf or the onion of the onion can provide an immunostimulating agent containing an immunostimulating component with 2 scallions or onions. [Embodiment] Forms for carrying out the invention 201132291 [Immunogenic activator] The immunostimulating agent according to the present invention contains the leaf body portion extracted from the leaf body of the onion or the onion (excluding the edible portion (the bulb portion) An immunostimulating component that contains a part of the leaf stalk and the leaf body. The onion used in the present invention refers to a plant belonging to the genus Allium of the family Liliaceae, and is represented by the Allium fistulosum<scientific name. The S used in the present invention may be roots, or may be a material, and the variety, cultivation method, collection period, and the like are not limited. As the root scallions, you can cite the onions of the Kagawa Kagawa, the onions of the Kanda group under the Inada, the onion of the Chisato group, the black onion, the onion, the onion, the onion, and the onion. Nine groups of Yuejin-based onions, late pomelo onions, non-liming onions, red onions, etc. In addition, as the scallions, you can cite the nine groups of nine stalks of the scallions, the nine groups of nine scallions, the nine sages of the filial piety, the saga of the Kaga group, and the non-moisturizing Onion, viviparous onions, etc. In addition, in addition to the above, it is also possible to use the onion which is native to China, Mongolia, and the like. The onion is mainly composed of the green leaf body, the white leaf, and the root. When the roots are stalked, the leaf sheath portion to be eaten is rubbed into the soil, and cultivated in an unilluminated manner, and the leaf body portion is discarded after harvesting. In the present invention, in particular, an immunostimulating component contained inside the leaf body of the onion is used. Therefore, according to the present invention, the leaf body of the root stalk which is usually discarded can be effectively utilized. The immunostimulating component of the present invention is less contained in the leafy portion of the onion as shown in the examples described later. The immunostimulating component in the present invention is obtained from the immunostimulating component of the body of the leaf of Huihui by using 201132291, and compared with the field using the whole benefit, the extract of the immunostimulating component becomes useless after the extraction, == immunostimulating component. In addition, 'immunization from the body of the onion leaves to form a knife, compared with the immune revitalizing ingredients from the leaves of the onion, because of the high immune activation effect, through the use of immunostimulants, can be more effective The immune revitalization effect. The foreign genus used in the present invention is a plant belonging to the genus Allium, and refers to the scientific name of Amum cepa. Yanghui used in the present invention is not limited to its variety or planting method, collecting shaft #黄葱,赤洋1, white onion. The example is made up of the leaf body, the bulbous part (the leaf part of the globular part of the edible glutinous part, and the root part), and the leaf part and the leaf part of the leaf part are not included. In the present invention, in particular, an immunostimulating component contained in the stems and leaves of Yanghui is used. Thus, the immune-stimulating component is extracted from the stems and leaves which are usually discarded, and the scales are not eaten. The lean beta becomes useless, and the immune-stimulating component can be extracted more efficiently. In addition, the immune-stimulating component extracted from the stems and leaves of the artichoke is not more effective than the immune extract from the bulbous part of the onion. The immune revitalizing effect is 'immune from the body of the onion leaf. The imitation has a very strong immune revitalizing effect. Therefore, it can be used more easily by using it in the immune activator'. In the present invention, the immunostimulating component is extracted from the leaf body of the onion or the k-leaf of the ocean. The method of extracting the immune-stimulating component from the leaf body of the self-help leaf or the stem and leaf of the onion is not limited. , can be exemplified by water extraction method, 2011322 91 hot water extraction method, pressurized hot water extraction method, supercritical extraction method, etc. As shown in the examples described later, according to the water extraction method, the yield of the obtained immunostimulating component is excellent due to the high yield of the immunostimulating component. In particular, as shown in the examples below, the immunostimulating effect of the immunostimulating component obtained by the water extraction method is much higher than that obtained by the hot water extraction method. The water extraction method is based on water. The solvent is a method of dissolving a soluble component in water. The temperature of the water to be used is not particularly limited as long as it is lower than 70° C. The immunostimulating component is extracted with water from the leaf body of the onion or the stem and leaf of the onion. In the case of the method of extracting, for example, the leaf part of the onion or the stem and leaf part of the onion is cut in the longitudinal direction, and the water is allowed to permeate the cut surface, and then the external force is applied by hand-tightening the cut surface to make the immune active ingredient It is preferable to use a machine formed by such a mechanism, etc. In the case of extraction by a water extraction method, the extract containing the obtained immunostimulating component is a leaf body. The content of the immunostimulating component which is not a substance and has an active ingredient is high, and the immunostimulating component extracted by the water extraction method has almost no odor which is characteristic of allium plants and is odorless, colorless and transparent. The hot water extraction method uses a hot water having a higher temperature than the water used in the water extraction method as a solvent to dissolve the soluble component in hot water. The hot water temperature used is 70 to 100 ° C, preferably. It is preferably from 90 to 100 ° C. Further, the extraction time is from 10 minutes to 30 minutes, preferably from the leaves of the onion, or the stems and leaves of the onion, by extracting the immune activating component by hot water extraction, the leaf body Or the treatment of stems and leaves is not particularly necessary. It is advisable to decoct the body or stems and leaves of the leaves 201132291 under the above conditions, and it is preferable to recover the boiled juice. Moreover, in the case of the hot water extraction method, compared with the water extraction method, the immunity is activated. Since the residual amount of the component in the tissue is large, it is more preferable to further extract the extract residue, juice, and the like. In addition, when extracting by the hot water extraction method, the extract containing the obtained immunostimulating component contains a large number of tissues of the leaf body, and the total amount of the extract is large, but the content of the immunostimulating component which is an active ingredient is low. It has a pungent odor characteristic of allium plants and is a low-viscosity substance which is turbid in brown. Further, the immunostimulating component may be further dried after extracting from the leaf body of the onion or the stem and leaf of the onion. The method for drying the extracted immunostimulating component is not particularly limited. For example, the extract may be filtered, and then dried, dried, drum dried, spray dried, etc. Dry method, etc. When the extract is dried by a water extraction method, an odorless and finely divided white powder can be obtained. On the other hand, when the extract is dried by the hot water extraction method, a slightly brown powder which is irritating to the odor and coarse particles is obtained. By drying the immunostimulating component, the use and management of the immunostimulating component is facilitated. The immunostimulating component contained in the immunostimulating agent of the present invention is contained in the leaf body of the onion or the stem and leaf of the onion, and is administered in vivo to increase the immunity in the living body. The immunostimulating component is extracted by extracting from the leaf body of the onion or the stem and leaf of the onion. The immunostimulating component is water-soluble, and by utilizing this property, water, hot water or the like can be used as a solvent for extraction. Further, the extracted immunostimulating component has heat resistance. The immune revitalizing component extracted from the leaf body of the onion or the stem and leaf of the onion is different from the method of 201132291. When extracted by the water extraction method, the irritating odor characteristic of the two plants is odorless and is unique to plants. Two: The hot water and the like do not contain the leaf body tissue other than the immune flight component. The f's extract by the water extraction method differs from its physical properties. The immunostimulating component contained in the immunostimulating agent according to the present invention promotes the production of cytokines or promotes (4) the swallowing action of cells, thereby enhancing the immunity of the living body. That is, the immunostimulating component activates the immune mechanism in vivo, especially in comparison to the early initial immunoreaction of antibody production. In addition, in the present specification, the term "immune activation" refers not only to the effect of activating the immune function of the living body, but also to the case where the tilting ability of the living body is lowered based on the age, the young age, the disease, the pressure, etc. The effect of the function returning to the normal state. Whether or not the immunostimulating component has an immunostimulating action can be confirmed by, for example, comparing the amount of cytokine produced in the living body of the immunostimulating agent according to the present invention with the amount of cytokine produced in the living body in which the immunostimulating agent is not administered. The immunostimulating agent according to the present invention is administered orally or parenterally in the living body to allow the immunostimulating component to be absorbed in the living body. In vivo, polymorphonuclear leukocyte (PMN) or macrophage cell line is responsible for the blood flow t run and patrol the whole body of the 201132291 - the phase of the invention - the immune component contained in the immune activity, in the ''JL mouth The U if shape is absorbed in the state of maintaining activity, and the blood is captured by the blood. #王身' promotes the production of cytokines by white blood cells, which enhances the swallowing effect of the giant cell. The immunological bribe H(4) activity according to the present invention is expressed, for example, by the amount of TNF (tumor necrosis factor)-α produced in a living body to which an immunostimulating component is administered (pg/mL). The immunostimulating component of the immunostimulating agent according to the present invention is orally administered as an effect, and is exemplified by the following description, and is also likely to be reproduced even if it is in vitro, conveniently in RAW264 cells, j 774> When the evaluation target (sample) is added to the culture system of the mega-cell line cell line such as a cell, the amount of inflammatory cytokine produced by _α or the like can be used as an index. Specifically, the cells and the like are adjusted (1×105 cells/200 μΐν pores), and then seeded in a 96-well plate for several hours of pre-incubation, and washed with pBS( ) or the like to be added to the FCS (fetal calf serum). # RpMn64〇, E, MEM #Mechanical sample (1~l〇〇(WmL), after 18 hours, the culture supernatant was collected. ± The inflammatory cytokines of the TNF_af of the clear liquid towel (enzyme immunoassay), organism Analyze (bi〇assay), etc. Further, the experimental facilities or actual listening parts used in the experimental conditions (4) may be appropriately arranged, for example, when the amount of the sample is specified as 1 mg/mLy, the cytokine activity may also be used as Indicators for classification management, regulation, etc. In addition, when the specification is 値, the activity is fluctuating due to the culture conditions and state of the cells, and the negative control (medium only) or the positive control group is designed. (P〇sitive control) (LPS (polysaccharide), etc.) is preferred for comparison with the activity of 12 201132291, etc. Further, it is not limited to TNF α ' or il (interleukin ( Interieukin)), il-6, IL-12 MCP (mononuclear ball chemotaxis) Other substances such as white matter, m〇n〇cyte chem〇tactic pr〇tein)-1 and other inflammatory cytokines are used as indicators, and are not limited to other experiments in this experimental system, such as “timer (4) 丨time pcR). The system grasps the amount of cytokine gene expression. The immunostimulating agent related to the present invention is an immunostimulating component which is mixed with a pharmaceutically acceptable preparation and can be administered orally as a lozenge, a pill, a granule, a capsule, an agent, a medicinal material, or It can be administered as an injection, a sublingual ingot, etc., intravenously, subcutaneously, and intramuscularly. The carrier of the company can use various organic or wall materials which have been conventionally used. For example, excipients, lubricants, binders, disintegrating solvents, dissolution aids, suspending agents, isotonic agents, and = agent, etc. Also, the immune thief live agent, as necessary: d, antioxidants, 荽 丨 丨 male I million rot J color agent, sweetener and other additives. The immune activity in the in vivo administration of the immunostimulant becomes the second activity of the target:::Remove:=The amount of sputum can be appropriate according to the degree of sputum effect, etc. a suitable, choose. Also, even in the choice of immune agents. The method of the present invention is intended to be effective for the purpose of the immunostimulating effect. The weight of the object can be adjusted to the weight of the object. For example, the amount of the tableting agent can be, for example, at least i曰~2 0, which can be 〇.3~3. During the period of administration, the immune system in the body of the invention is related to the immune activity of the invention, and it plays a role in the immune response in the relative initial stage of 1st, so 13 201132291 is in vivo, and its immune revitalization effect is 3 The immunostimulant can prevent the elimination of various cancer cells based on the infection or stress, the disease, and the immunity of the living body. The various diseases are used to improve the elimination of the early cancer cells, and the elimination of the sugar, the old waste. Exclusion and other effects. More specifically, the present invention relates to a vaccine for a mammal (eg, mouse, cat, dog, cow, horse, sheep, goat, family, human, etc.), birds, fish, molluscs (shellfish, etc.) It can be used for a variety of bacteria, true g, viral infections, etc. = treatment or prevention. For example, if the present invention is administered to a mammal with a tumor, the life-extending effect can be exerted. 0 As a target disease in which the immunostimulating agent related to the present invention can be used, various leukemias, malignant lymphomas, osteosarcoma, malignant melanoma, malignant villus epithelium, muscle tumor, ovarian cancer, uterine cancer, prostate cancer, pancreatic cancer, and the like can be exemplified. a viral tumor such as a stomach, a bowel, a lung cancer, an esophageal cancer, a head and neck cancer, a brain tumor, or a liver cancer, etc., but is not limited thereto. The immune activity associated with the present invention is pre-ingested before infection by bacteria or viruses. In the case of the agent, it is possible to obtain a so-called refractory infection, and even if the infection is easy to treat, it is also possible to prevent the infection. Therefore, the immunostimulating agent related to the present invention can also be used as the agent. A health food or a pharmaceutical product 'allows a living body to be ingested daily. Further, the immunostimulating agent of the present invention' can also be used in a breeding feed such as birds, fish, molluscs (shellfish, etc.). The immune revitalizing component containing the leaves and leaves extracted from the onion, or the stems and leaves of the onion, has a higher immunostimulating effect than the case of extracting from onion or onion 14 201132291. Compared with the case where all the immune-stimulating components are extracted from the sputum or the ocean, the extract becomes less useless, and the immune-enhancing component can be extracted more efficiently. In particular, when using kenshen 4' or using onions, since it can be recycled, it has not been eaten. The abandoned parts can reduce the cost of waste and the effective use of resources. 0 Now, especially in the large-scale production of root stalks, harvest the onions with automatic onion harvester, through automatic root cutting machine, automatic peeling machine, automatic leaf cutting The machine is processed and normalized. Even if the origin of such a system is not introduced, the leaf body is cut and discarded at the farm or collection site. According to the present invention, the utilization of unused resources can be promoted by utilizing such a portion that is usually discarded, and because of the self-reinforcing leaf body or onion The extraction method of the active ingredient in the stems and leaves is also made possible by the existing facilities, and it is easy to industrialize. Therefore, it also links the reduction of waste, the promotion of recycling, and the industrialization of agricultural by-products. Immune revitalizing ingredients can be used in the health food industry, and are useful for various disease prevention
It報普及，與蔥及洋蔥之消費擴大連繫，可寄與農業之活 性化。 Μ 〔免疫賦活劑的製造方法〕 本發明相關的免疫賦活劑之製造方法包含自蔥之葉身 部、或洋蔥之莖葉部萃取免疫賦活成分的萃取步驟。於本 發明相關的免疫賦活劑之製造方法所萃取的免疫賦活成分 ，因為與含於上述的本發明相關的免疫賦活劑的免疫賦活 15 201132291 成分相同，省略其詳細説明。 (萃取步驟） 於本發明相關的免疫賦活劑之 身部、或洋葱之莖葉部萃取免疫賦:::作 賦活:八^中自蔥之葉身部、或洋蔥之莖葉部萃取免疫 未制限定，可採用於上述免疫賦活劑 項中列舉的卒取方法。於萃取步驟，經由上述任一種萃 取方法’使用可溶溶劑將免疫賦活成分自惠之葉身部、或 洋蔥之莖葉部使免疫賦活成分溶出為宜。 於萃取步驟所得萃取物，於使用水萃取方法的情形， 幾乎沒有S屬植物财的雜臭味，為無色透明之黏性物 質又，使用熱水萃取方法的情形，於萃取步驟所得的萃 取物’具有S屬植物特有的刺激臭味，為茶色且混濁的低 黏度物質。於萃取步驟’經水萃財法萃取的萃取物中所 含免疫賦活成分以外不要物y之含量，亦較經由其他方法 者少，因有效成分的免疫賦活成分以高產率獲得而為較佳 又，於萃取步驟自蔥之葉身部、或洋蔥之莖葉部獲得 萃取物後，將蔥或洋蔥之萃取殘渣以均質機榨汁等，可進 一步取出於萃取殘渣中殘存的免疫賦活成分。 (乾燥步驟） 再者，本發明相關的免疫賦活劑之製造方法，亦可含 有於萃取步驟萃取免疫賦活成分後，進一步使該免疫賦活 成分乾燥的乾燥步驟。作為使萃取的免疫賦活成分乾燥的 16 201132291 方法’可採用於上述免疫賦活劑項目中列舉的乾燥方法。 於經水萃取方法萃取的萃取物之乾燥步驟使乾燥的情 形’：獲得無臭且顆粒細的白色粉末。另一方面，於經由 熱水萃取料萃取的萃取物之乾齡雜乾燥的情形，獲 得些許刺激臭、顆粒粗的茶色粉末。 依據本發明相關的免疫賦活劑之製造方法，因自惠之 葉身部、或洋f之莖葉部萃取免疫賦活成分，可萃取更高 免疫賦活作狀免疫賦活成分。χ，依據本㈣相關的免 疫賦活劑之製造方法’與自蔥或洋f全體萃取免疫賦活成 分的情形比較，萃取後成為無用的部分少，可更有效率萃 取免疫賦活成分。 〔食品組成物〕 本發明相關的食品組成物含有萃取自葱之葉身部、或 净惠之莖葉部的免疫賦活成分。x ’本發明相關的食品組 成物亦可進—步含有該免疫賦活成分以外之添加劑。本發 明相關的食品組成物所含的免疫賦活成分因與上述本發明 相關的免疫賦活劑所含的免疫賦活成分相同，故省略其詳 細説明。 作為本發明相關的食品組成物所含的免疫賦活成分以 外之添加齊彳’/、要不損&免疫賦活成分之免疫賦活效果， 可為任意所欲的成分，例如，可舉例pH調整劑、有機酸 、糖醇、甘味料、香料、維生素類、骨代謝維生素類、抗 氧化劑、賦形劑、可溶化劑、結合劑、潤滑劑、懸浮劑、 濕潤劑、皮膜形成物質、矯味劑、矯臭劑、著色料、保存 17 201132291 劑、抗菌劑、殺菌劑、抗炎症劑等之食品組成物中通常使 用的添加劑。 本發明相關的食品組成物經由於活體經口攝取，於該 活體内使免疫賦活成分吸收，而使該活體内的免疫機能增 強。因此，該食品組成物較佳為經口攝取容易的形態，例 如’可為菱形錠(lozenge)、錠劑（tablet)、膠囊、顆粒、粉 末汁、橡皮糖(chewing gum)、糖果(candy)、橡皮糖(Gummi candy)等之形態。又，本發明相關的食品組成物亦可配合 鳥類、魚類、軟體動物（貝類等）等之養殖用飼料中使用 〇 本發明相關的食品組成物可混合由上述免疫賦活成分 之萃取方法所萃取的免疫賦活成分與上述添加劑而製造。 又’免疫賦活成分與添加劑可同時或各別混合，其形態或 劑型可依向來習知的方法調製。 〇本發明相關的食品組成物所含免疫賦活成分之量視食 、且成物之形態、攝取的活體之年齡、體重、性別、作為 關的的免疫賦活效果之程度等可適宜設定。又，本發明相 的食°°組成物之攝取量及攝取期間，可適宜設定，但例 ^、'相對於投與對象之體重lkg ’每1日為〇·3〜3mg，至 曰2曰間攝取為宜。 老化本發明相關的食品組成物可節基於因感染或壓力、 於提疾病等活體免疫能力降低所認為的各種疾病，使用 ^ j仞期癌細胞之排除、過剩脂質或糖質之排除、自己 排除等之作用。於細菌或病毒感染等之前預先攝取 201132291 本發明相關的人σ 使感染亦成物時，可獲得所謂難感染，進而即 的食品組成4冶療的預防作用。因此，亦可將本發明相關 本發9句作為健康食品或醫藥品於活體每日攝取。 健機能食2相關的食品組成物包含含有免疫賦活成分的保 _食品定保健用食品、營養機能食品等）及營養 物，含有將明的健康食品。又，本發明相關的食品組成 用而：C活成分作為飲食物品製造原料之-者使 #八彳康食品等一般食品之外，亦包含將免疫賦活 (特77定保健製造原狀—所個調製的⑽機能食品 食°°、營養機能食品等。 、孫苴~ 保健用食品（包含附條件的特定保健用食品 的嫩二/⑼等上可表現日本㈣生㈣省承認或認可 、、广:t呆健用途的食品。於本發明中特定保健之目的為 免疫=之活性化，具體而言為免疫機能降低為起因的疾 ^ '、及T善，作為表現之一例，可舉例相關法規許可 之^圍2之「免疫賦活作用」。於特定保健用食品，如此表 示若被認可，因可謀求與—般食品之差別化，為本發明相 關的食品組成物之一較佳態樣。 本發明相關的食品組成物因包含自蔥之葉身部、或洋 蔥之鱼=邻萃取的免疫賦活成分，可提供短期間之攝取即 更有效率獲得免疫賦活效果的食品組成物。又，該食品組 ，物所3免疫碑活成分因自;I、之葉身部、或洋g之莖葉部 萃取:與自S或洋£全料取的情形峰，萃取後成為無 用的口P刀 >，可效率佳利用經萃取的免疫賦活成分。 19 201132291 〔免疫賦活方法〕 本發明相關的免疫賦活方法包含投與自蔥之葉身部、 或洋蔥之莖葉部萃取的免疫賦活成分之投與步驟。本發明 相關的免疫賦活方法所使用的免疫賦活成分因為與上述本 發明相關的免疫賦活劑所含的免疫賦活成分相同，故省略 其詳細説明。 於本發明相關的免疫賦活方法之投與步驟，免疫賦活 成分可於活體内經口或非經口的投與。於該投與步驟於活 體（投與對象）内投與的免疫賦活成分，於活體内被吸收 ，經由血流運送到末梢組織，促進細胞激素之生成，而使 巨喔細胞之吞食作用提升。 於本發明相關的免疫賦活方法之投與步驟，免疫賦活 成分可與藥學上可容許的載體一起於活體内被投與。又， 投與的免疫賦活成分之形態並未特別限定，可為錠劑、丸 劑、顆粒劑、膠囊劑、糖漿劑、乳劑、懸浮劑等經口投與 ，或亦可作為注射劑、栓藥、舌下錠等經靜脈内、皮下及 肌肉内等非經口投與。作為藥學上可容許的載體，可採用 於上述免疫賦活劑項目中列舉者。 於本發明相關的免疫賦活方法之投與步驟，經口或非 經口的活體内投與的免疫賦活成分之投與量，依據投與之 對象的活體之年齡、體重、性別、作為目的的免疫賦活效 果之程度等可適宜選擇。又，於免疫賦活成分之投與期間 ，可以獲得作為目的的免疫賦活效果而適宜選擇。於本發 明相關的免疫賦活方法之投與步驟，免疫賦活成分之投與 20 201132291 量，^如’相對於投與對象體重啦，每U ，杈與期間’例如可為至少1日〜2日。〇 ·、、、〇·3〜3mg 依據本發明相關的免 自蔥之葉身部、戈洋苗二♦/ 1 ，因於投與步驟投鱼 被投與的活體内，於L =葉部萃取的免疫賦活成分，^ 果。又’經投與的免疫賦;有：率地獲得免疫贼活致 洋葱之里葉部萃取者， :萬之葉身部、或 較’萃取後變無 取的情形作 賦活成分。 心少，可有”彻轉取的免疫 以白μ ;本t明相關的免疫賦活劑，上述#广β 較佳。此外，4::、或上述洋葱之莖葉部經水萃取:： 成分為黏性物質者較:相關的免疫賦活劑，上述免疫賦活 Γ免疫賦活劑，=免者广於本發明相 較佳，更佳為白色之粉f賦活成分叫取後經乾燥者為 此外，本發明知 成或-細胞之吞食劑會促進細胞激素之生 外，二==:::Γ_σ投與者較佳，此 2日間投與者較佳。 g母1日〇·3〜3mg至少1 取步驟將上==目㈣免疫賦活劑之製造转件於上 成分以水萃取者較佳。 態 μ下陳不實施例， 進一步詳細說明本發明之實施形 21 201132291 [實施例] (1 :免疫賦活成分之萃取） 於農研機制蔬菜茶業研究所（三重縣津市）栽培12 個月（2008年4月8曰播種、2_年4月8日收穫），萃 取處理1日前將收穫的下仁田葱之葉身部於長度方向垂直 刀斷於約2GC之蒸館水巾將此切斷面全體浸潰3〇分鐘 ^葉f部料拉直的方式，吸水成為膠狀的葉身内部之成 分削落後’將葉身部纟且織取出，殘餘的液體以口徑丨麵之 耐=網(nyl_esh) (SANPLATEC公司製）過滤。經過渡所 獲知的液體為有-定黏度之黏液，不具有祕有的刺激臭 ’且為無色透明。 將此黏液移到銘盤，於_2(rc練結後，於eyela製棚 式束結乾燥機(FD_55G)乾燥2日。此結果，獲得η個來自 2田蔥（5.5kg:内葉身部為3.2kg)約38 5g之柬結乾 、’ 所得;東I。乾燥物為非常輕的海錦狀，容易粉碎，生 成的粉末為白色。 (2:細胞激素生成之促進） 〜以5週齡取自日本SLC，m週預備飼育後，體重29 :3解2二 雄性小鼠’將上述h所得粉末l〇mg * ^ ( n：； ^ ^ Μ (sonde)^ .實施3次同樣實驗）。對照組之小鼠同樣The popularity of the It newspaper is linked to the consumption of onions and onions, which can be used to activate agriculture. 〔 [Method for Producing Immunostimulating Agent] The method for producing an immunostimulating agent according to the present invention comprises an extraction step of extracting an immunostimulating component from the leaf body of the onion or the stem and leaf portion of the onion. The immunostimulating component extracted by the method for producing an immunostimulating agent according to the present invention is the same as the immunostimulating agent of the immunostimulating agent according to the present invention described above, and the detailed description thereof will be omitted. (Extraction step) Extracting immunity from the body of the immunostimulating agent related to the present invention or the stem and leaf of the onion::: for activating: eight^ from the leaf body of the onion, or the stem and leaf of the onion The method can be used as the stroke method listed in the above immunostimulating agent. In the extraction step, it is preferred to use a soluble solvent to elute the immunostimulating component from the leaf body of the immune-promoting component or the stem and leaf of the onion by any of the above extraction methods. The extract obtained in the extraction step, in the case of using the water extraction method, has almost no odor of S plant genus, is a colorless and transparent viscous substance, and in the case of using a hot water extraction method, the extract obtained in the extraction step 'It has a pungent odor characteristic of S genus, and it is a brown and turbid low-viscosity substance. In the extraction step, the content of the undesired substance y other than the immunostimulating component contained in the extract extracted by the water extraction method is less than that of other methods, and the immunostimulating component of the active ingredient is preferably obtained in a high yield. After extracting the extract from the leaf body of the onion or the stem and leaf of the onion, the extract residue of the onion or the onion is juiced by a homogenizer, and the remaining immunologically active component in the extraction residue can be further taken out. (Drying step) Further, the method for producing an immunostimulating agent according to the present invention may further comprise a drying step of further drying the immunostimulating component after extracting the immunostimulating component in the extracting step. As a method of drying the extracted immunostimulating component, the method of the present invention can be applied to the drying method listed in the above immunostimulating agent. The drying step of the extract extracted by the water extraction method gives a dry condition': a white powder which is odorless and finely divided is obtained. On the other hand, in the case where the dry extract of the extract extracted via the hot water extract is dry, a slight brown powder which is irritating to the odor and coarse particles is obtained. According to the method for producing an immunostimulating agent according to the present invention, the immunostimulating component can be extracted by extracting an immunostimulating component from the leaf body of the self-help leaf or the stem and leaf of the foreign f. χ, according to the manufacturing method of the immunosuppressive agent related to the above (4), compared with the case of extracting the immune activating component from the whole onion or the ocean f, the use of the extracting portion after use is less, and the immune activating component can be extracted more efficiently. [Food Composition] The food composition according to the present invention contains an immunostimulating component extracted from the leaf body portion of the onion or the stem and leaf portion of the net. x ' The food composition of the present invention may further contain an additive other than the immunostimulating component. The immunostimulating component contained in the food composition according to the present invention is the same as the immunostimulating component contained in the immunostimulating agent according to the present invention, and detailed description thereof will be omitted. The immunostimulating effect of the immunostimulating component other than the immunostimulating component contained in the food composition according to the present invention may be any desired component, and for example, a pH adjusting agent may be exemplified. , organic acids, sugar alcohols, sweeteners, spices, vitamins, bone metabolism vitamins, antioxidants, excipients, solubilizers, binders, lubricants, suspending agents, wetting agents, film forming substances, flavoring agents, A odorant, a coloring material, and an additive commonly used in a food composition such as a 201132291 agent, an antibacterial agent, a bactericide, an anti-inflammatory agent, and the like. The food composition according to the present invention is orally ingested in a living body, and the immune activating component is absorbed in the living body to enhance the immune function in the living body. Therefore, the food composition is preferably in a form that is easily taken orally, for example, 'may be a lozenge, a tablet, a capsule, a granule, a powdered juice, a chewing gum, a candy. , Gummi candy and other forms. Further, the food composition according to the present invention may be used in a breeding feed such as a bird, a fish, a mollusc (shellfish, etc.), etc. The food composition related to the present invention may be mixed and extracted by the extraction method of the above-mentioned immunostimulating component. The immunostimulating component is produced by the above additives. Further, the immunostimulating component and the additive may be mixed simultaneously or separately, and the form or dosage form thereof may be prepared according to a conventional method. The amount of the immunostimulating component contained in the food composition according to the present invention can be appropriately set depending on the form of the food, the form of the product, the age, body weight, sex of the living body, the degree of the immune activating effect, and the like. In addition, the intake amount and the ingestion period of the composition of the present invention can be appropriately set, but the example is that the weight lkg of the target is 〇3 to 3 mg per day, to 曰2曰. Intake is appropriate. Aging The food composition related to the present invention can be based on various diseases considered to be caused by a decrease in the immunity of living organisms such as infection or stress, and the introduction of diseases, and the elimination of cancer cells, the elimination of excess lipids or saccharides, and self-exclusion. Wait for the role. Pre-ingestion before bacterial or viral infection, etc. 201132291 When the human σ related to the present invention causes infection, it is possible to obtain a so-called refractory infection, and further, the food composition 4 is a preventive action. Therefore, it is also possible to take the nine sentences of the present invention as a health food or a pharmaceutical product for daily ingestion in a living body. The food composition related to the health machine can include a health food containing a immune-enhancing ingredient, a food for health care, a nutritious food, and the like, and a nutrient containing a healthy food. Further, in the food composition according to the present invention, the C-active ingredient is used as a raw material for the production of food and beverages, and the general food such as the Yakangkang food is also included in the preparation of the medicine. (10) Functional foods, foods, nutrition, foods, etc., Sun Wei~ Health foods (including the second-grade foods containing certain conditions of health care products, etc.) can be expressed in Japan (4) students (4) Provincial recognition or recognition, and wide: In the present invention, the purpose of the specific health care is the activation of the immunization, specifically, the reduction of the immune function as the cause of the disease, and T, as an example of performance, for example, the relevant regulations permit In the case of a specific health food, it is indicated that, if it is approved, it can be differentiated from the general food, and it is a preferred aspect of the food composition related to the present invention. The food composition according to the present invention can provide a food composition which is more effective in obtaining an immune revitalizing effect by ingesting for a short period of time because it contains the immune-stimulating component from the leaf body of the onion or the fish of the onion. , the food group, the biological site of the immunization of the tablet is derived from; I, the body of the leaf, or the stem and leaf of the ocean g: the peak of the situation taken from the S or the ocean, after extraction, becomes a useless mouth P knife> can efficiently utilize the extracted immunostimulating component. 19 201132291 [Immunization Revitalization Method] The immunostimulating method according to the present invention comprises administering an immunostimulating treatment derived from the leaf body of the onion or the stem and leaf of the onion. The administration step of the component. The immunostimulating component used in the immunostimulating method according to the present invention is the same as the immunostimulating component contained in the immunostimulating agent according to the present invention, and thus detailed description thereof will be omitted. In the method of administration, the immunostimulating component can be administered orally or parenterally in the living body. The immunostimulating component administered in the living body (administered subject) is absorbed in the living body through the administration step. The blood flow is transported to the peripheral tissue to promote the production of cytokines, and the phagocytosis of the giant scorpion cells is enhanced. In the administration step of the immunostimulating method related to the present invention, the immunostimulating component can be The pharmaceutically acceptable carrier is administered together in vivo. Further, the form of the immunostimulating component administered is not particularly limited, and may be a tablet, a pill, a granule, a capsule, a syrup, an emulsion, a suspension, or the like. Oral administration, or may be administered as an injection, a suppository, a sublingual tablet, etc., intravenously, subcutaneously, intramuscularly, etc. as a pharmaceutically acceptable carrier, which can be used in the above-mentioned immunostimulating agent project. The administration step of the immunostimulating method related to the present invention, the administration amount of the immunostimulating component administered orally orally orally, according to the age, body weight, sex, and the living body of the subject to be administered The degree of the immunostimulating effect or the like may be appropriately selected. Further, during the administration of the immunostimulating component, a desired immunostimulating effect can be obtained and appropriately selected. The administration procedure of the immunostimulating method according to the present invention, immunization The amount of active ingredient is 20 201132291, ^ such as 'relative to the weight of the subject, every U, 杈 and period' can be, for example, at least 1 day ~ 2 days. 〇·,, 〇·3~3mg According to the invention, the leaf body part of the stalk-free body, Geyang Miao 2 ♦/ 1 , due to the investment step, the fish is administered in the living body, at L = leaf part Extracted immune revitalizing ingredients, ^ fruit. In addition, the immune nucleus is administered; there is: the rate of getting the immune thief to live in the leaf extract of the onion, the body of the leaves, or the situation after the extraction is not available. If there is less heart, there may be "immunization with the white blood. The immunostimulating agent related to the present invention is preferably the above-mentioned #广β. In addition, 4::, or the above-mentioned onion stems and leaves are extracted by water:: For the viscous substance: the related immunostimulating agent, the above-mentioned immune stimulating Γ immune activator, = free of the invention is better than the present invention, and it is better that the white powder f is a living ingredient called after the dried, in addition, The invention or the cell-derived agent can promote the growth of the cytokine, and the second ==:::Γ_σ is preferred, and the donor is preferred during the 2 days. g mother 1 day 〇·3~3mg at least 1 The step of taking the upper == target (4) immunostimulating agent is transferred to the upper component and is preferably extracted with water. The state is not described in the embodiment, and the embodiment of the present invention is further described. 21 201132291 [Examples] (1) : Extraction of immune revitalizing ingredients) Cultivation in the Research Institute of Vegetable Tea Industry (Tsujin, Mie Prefecture) for 12 months (planted on April 8, 2008, harvested on April 8th, 2008), extraction treatment will be 1 day before The harvested stalk of the scallions of the stalks of the stalks of the stalks of the stalks of the stalks of the stalks 3 〇 minutes ^ leaf f material straightening, water absorption into a gel-like part of the inside of the blade after the cut off 'the body of the blade and woven out, the residual liquid with the diameter of the surface of the resistance = net (nyl_esh) ( Filtered by SANPLATEC Co., Ltd. The liquid obtained through the transition is a mucus with a certain viscosity, which does not have a secret odor and is colorless and transparent. Move this mucus to the dial, after _2 (rc practice, Dryed on the eyela shed bundle dryer (FD_55G) for 2 days. As a result, η came from 2 field onions (5.5kg: 3.2kg inside the inner body), about 38 5g of knots, 'gain; east I. The dried product is very light in the shape of a jelly, which is easily pulverized, and the resulting powder is white. (2: Promotion of cytokine production) ~ Taken from Japanese SLC at 5 weeks of age, after m weeks of preparation, weight 29:3 Solve 2 male mice 'The powder obtained by the above h l〇mg * ^ ( n:; ^ ^ Μ (sonde) ^. 3 times the same experiment). The mice in the control group are also the same
:經:投：二吣L自投與24小時間後再度同樣地將'蔥黏 附2又” 2次之投與3小時後，小鼠之腹腔中使用 注射針的5微注射器，投與冷卻至代的PBS(_)5mL 22 201132291 後’立即將其採集’獲得含腹腔細胞的腹腔液。經由注射 器之刻度得知採取的腹腔液之量（約回收3〜4mL；)。 其次’將採集的上述腹腔液離心，離心上清液以吸引 器（aspirator)除去後’以成為ΙχΙΟ6細胞/mL的方式添加游 離於10%FCS的RPMI1640中’於96孔平盤接種2〇〇μ[ 。於C〇2培養箱中培養18小時後，採取培養上清液，使 用ELISA套組（BioLegend公司製），測量TNF_a及IL12 之生成量。結果顯示於第1圖中之(a)及(b)。 如第1圖所示’於自2次經口投與蔥黏液的小鼠採取 的腹腔細胞’與對照組（第1圖中之(a)及(b)中的DDW) 比較，TNF-α及IL-12之生成量為有意義地增加。又，p <0·05表不以2標本t檢定為有意義差。 (3 :巨噬細胞之吞食作用之促進） 將與上述2.同樣地調整細胞數的腹腔液接種於96孔 平盤，添加來自酵母之酵母多糖(Zymosa)，且吞食之際於 450nm之波長發色的經特殊加工的試藥（Cyt〇Select : COSMO BIO公司製），調查其吞食作用。結果顯示於第2 圖。 如第2圖所示，於自2次經口投與蔥黏液的小鼠採取 的腹腔細胞，與對照組（第2圖中之DDW)比較，酵母 多糖之吞食能力為有意義地提升。 (4 .卒取部位之比較） 將免疫賦活成分之免疫賦活作用由其萃取部位比較。 首先，與上述1.相同地，自蔥之葉身部萃取黏液。萃取黏 23 201132291 液的葉身部之殘渣與葉鞘部各自均質化後，以口徑^職之 耐論網（SANPLATEC公司製）過渡。濾液經冷束乾燥使 其乾燥，各自獲得來自葉身部殘渣之粉末3 3g與來自葉鞠 部之粉末68.2g。 來自黏液之粉末、來自葉身部殘潰之粉末、及來自葉 鞘部之粉末，以各種濃度製作溶解於1〇%fcs_e,_mem培 養基的溶液，以調整為lxl〇5細胞/2〇〇μυ孔接種於％孔 平盤，各自添加於6小時前培養的巨嗟細胞系細胞株 RAW264 (講自理化學研究所生物來源中心、）之培養系統 。添加18小時後採取上清液，使用EUSA套組（Bi〇Legend 公司製）測量各種細胞激素量。結果顯示於第3圖中之⑻ 〜（c)。 如第3圖中之(a)〜所示，TNF_a (第3圖中之(a)) 、IL-6 (第3圖中之(b))、及MCIM (第3圖中之(c))任 者，對於含來自黏液之粉末的溶液，濃度會依續地增加 。因此，蔥所含免疫賦活成分顯示含於葉身内部之黏液中 。又，對於來自葉身部殘渣之粉末的溶液，因各細胞激素 ：!：稍微增加，可以說是經由殘渣中殘存的黏液作用所致。 又，第3圖中之星號係*表示p<〇 〇5、* *表示p< 0.01、* * * 表示 p<〇 〇〇1 (以 Dunnett 檢定）〇 (5 :耐熱性之檢討） 與上述1.同樣地，於附塑膠製蓋的試驗管令計取5mg 自蔥之葉身部萃取的免疫賦活成分，以蒸餾水使成為 Img/mL·的方式溶解。此等以各種條件（於〇〜1〇〇〇cl〇分 24 201132291 鐘’ 100°C 5〜6〇分鐘，高壓蒸氣滅菌器（autoclave))加熱 處理後，於-2(TC使凍結後，以EYELA製棚式凍結乾燥機 (FD-550)冷凍乾燥1日。於此等中添加10%FCS-E，-MEM 培養基，再度以成為lmg/mL的方式使溶解。此等以ixl〇5 細胞/200μΙ7孔之細胞濃度接種於96孔平盤’6時間前培養 的巨噬細胞系細胞株RAW264 (購自理化學研究所生物來 源中心）之培養系統中培養基交換後添加。添加18小時後 採取上清液，使用ELISA套組（BioLegend公司製），測量 TNF-α量。結果顯示於第4圖。 如第4圖所示，因TNF_a誘導活性以各種熱處理並未 失活’本免疫賦活物質顯示為耐熱性者。 (6 :萃取方法之比較） 免疫賦活成分之免疫賦活作用以其萃取方法作比較。 首先’以與上述1.同樣之方法自蔥（品種為吉蔵）之葉身 部將黏液作水萃取。所得粉末（水萃取物）之產率（對葉 身部重量）為0.32%。 其次’將以其他方式準備的上述蔥（品種為吉蔵）之 葉身部於100¾之熱水煮沸30分鐘。所得液體為無黏性， 有蔥特有的刺激臭味，茶色混濁。將此液體移至鋁盤，於 -20°C凍結後’以EYELA製棚式凍結乾燥機(FD-550)冷凍 乾燥2日。熱水萃取物之乾燥粉末有若干刺激臭味，茶色 、粒度粗、產率為2.37%。: 经: 投:二吣L self-injection and 24 hours later, the same 'the onion sticks 2 again' 2 times of the cast and 3 hours later, the mouse's abdominal cavity using the injection needle 5 micro syringe, the injection cooling After the passage of PBS(_)5mL 22 201132291, 'acquire it immediately' to obtain peritoneal fluid containing peritoneal cells. The amount of peritoneal fluid taken is determined by the scale of the syringe (about 3~4mL is recovered;) The above peritoneal fluid was centrifuged, and the supernatant was centrifuged and removed by an aspirator, and then added to RPMI1640 which was free of 10% FCS in a manner of ΙχΙΟ6 cells/mL. Inoculation was carried out in a 96-well plate at 2 μμ [. After culturing for 18 hours in a C〇2 incubator, the culture supernatant was taken, and the amount of TNF_a and IL12 produced was measured using an ELISA kit (manufactured by BioLegend). The results are shown in (a) and (b) of Fig. 1. As shown in Figure 1, the 'peritoneal cells taken from mice that were orally administered with onion mucilage' were compared with the control group (DDW in (a) and (b) in Figure 1), TNF- The amount of α and IL-12 produced was significantly increased. In addition, the p <0·05 table was not significantly deviated by the 2 specimen t. (3: Macrophage fine Promotion of the phagocytosis of the cells. Inoculation of the peritoneal fluid with the same number of cells as described above in a 96-well plate, addition of yeast-derived zymosan (Zymosa), and special coloring at a wavelength of 450 nm when swallowed The processed reagent (Cyt〇Select: COSMO BIO) was investigated for its swallowing effect. The results are shown in Fig. 2. As shown in Fig. 2, the abdominal cavity was taken from mice that were orally administered with onion mucilage twice. Compared with the control group (DDW in Fig. 2), the phagocytosis ability of zymosan was significantly improved. (4. Comparison of stroke sites) The immunostimulating effect of the immunostimulating component was compared by the extraction site. In the same manner as in the above, the mucus was extracted from the leaf body of the onion. The residue and the sheath and sheath of the leaf body of the 201132291 liquid were homogenized, and then the transition was carried out by the Naruto Net (made by SANPLATEC). The filtrate was dried by cold-dried drying, and each obtained 3 3 g of powder from the residue of the blade body and 68.2 g of powder from the leaf axils. Powder from mucus, powder from the body of the blade, and powder from the sheath portion To The solutions dissolved in 1%% fcs_e, _mem medium were prepared at various concentrations, and adjusted to lxl〇5 cells/2〇〇μυ holes inoculated on % well plates, and each was added to the python cell line RAW264 cultured 6 hours ago. (The self-organizing center of the Institute of Biochemistry, the biological source center). After 18 hours of addition, the supernatant was taken, and the amount of various cytokines was measured using an EUSA kit (manufactured by Bi〇Legend). The results are shown in Fig. 3 (8). ~(c). As shown in (a) to (Fig. 3), TNF_a ((a) in Fig. 3), IL-6 ((b) in Fig. 3), and MCIM ((c) in Fig. 3) Anyone, for solutions containing powders from mucus, the concentration will continue to increase. Therefore, the immune revitalizing component contained in the onion is shown to be contained in the mucus inside the leaf body. In addition, the solution of the powder derived from the residue of the leaf body is slightly increased by the cytokine:!: it can be said to be caused by the mucus remaining in the residue. In addition, the asterisk * in Fig. 3 indicates that p < 〇〇 5, * * indicates p < 0.01, * * * indicates p < 〇〇〇 1 (by Dunnett test) 〇 (5: heat resistance review) and the above 1. In the same manner, the test tube with a plastic lid was taken to obtain 5 mg of the immunostimulating component extracted from the leaf body of the onion, and dissolved in distilled water to obtain Img/mL·. These are subject to various conditions (in 〇~1〇〇〇cl〇 24 201132291 hrs '100°C 5~6〇 minutes, autoclave) after heat treatment, after -2 (TC makes freezing, The EYELA shed freeze dryer (FD-550) was freeze-dried for 1 day, and 10% FCS-E, -MEM medium was added thereto, and dissolved again to become lmg/mL. The cell concentration of the cells/200 μΙ 7 wells was inoculated into the culture system of the macrophage cell line RAW264 (purchased from the Institute of Biological Sources of the Institute of Physical and Chemical Research) cultured in a 96-well plate at the time of 6 hours, and then added after 18 hours of addition. The supernatant was measured for the amount of TNF-α using an ELISA kit (manufactured by BioLegend Co., Ltd.). The results are shown in Fig. 4. As shown in Fig. 4, the TNF_a-inducing activity was not inactivated by various heat treatments. (6: Comparison of extraction methods) The immunostimulating effect of the immunostimulating component is compared by the extraction method. First, the same as the above 1. The leaf body of the onion (variety is Jizhen) will be Mucus for water extraction. The resulting powder (water The yield of the extract (the weight of the leaf body) was 0.32%. Next, the leaf body of the above-mentioned onion (variety of yam) prepared in other manners was boiled in hot water of 1003⁄4 for 30 minutes. The obtained liquid was non-sticky. Sexual, with a stimulating odor characteristic of onion, brown turbid. Move this liquid to an aluminum pan and freeze at -20 °C. Freeze-dry for 2 days with EYELA shed freeze dryer (FD-550). The dry powder of the material has a certain irritating odor, brown color, coarse particle size, and a yield of 2.37%.
又’熱水萃取後之萃取殘〉查中因有活性成分的黏液殘 存’將其均質化後’以口徑1mm之耐綸網（SANPLATEC 25 201132291 公司製）過濾，所得濾液經冷凍乾燥而乾燥，獲得粉末。 熱水萃取（殘渣）之粉末之產率為2.79%。 製作將水萃取物、熱水萃取物、及熱水萃取（殘渣） 以各種濃度使溶解於10%FCS-E’-MEM培養基的溶液，調 整為lxlO5細胞/200μί/孔，接種於96孔平盤，各自添加 於6小時前培養的巨噬細胞系細胞株RAW264 (購自理化 學研究所生物來源中心）之培養系統。添加18小時後採取 上清液，使用ELISA套組（BioLegend公司製）測量TNF-a 量。結果顯示於第5圖。 如第5圖所示，添加水萃取物時之TNF-a量，較添加 熱水萃取物、及熱水萃取（殘渣）時之TNF-a量更高，比 活性（獲得同等之活性所必要的必要量）為約10倍亦較高 。於上述4.，黏液以外不含免疫賦活成分，又，於上述5. ，顯示本免疫賦活成分為财熱性，熱水萃取物及熱水萃取 殘渣之活性較水萃取物之活性差，被認為亦萃取與活性無 關的成分。因此，本免疫賦活成分亦可經由熱水萃取來萃 取，但所得萃取物不僅性狀及風味完全不同，活性亦變低 〇 (7:來自蔥之免疫賦活成分與來自洋蔥之免疫賦活成 分之比較） 將免疫賦活成分之免疫賦活作用，由萃取的植物作比 較。首先，與上述1.同樣自蔥之葉身部將黏液作水萃取， 所得粉末（水萃取物）之產率（對葉身部重量）為0.32% 26 201132291 又三自洋蔥之莖葉部亦以同樣方法進行萃取，所得粉 末（水萃取物）之產率（對莖葉部重量）為！.⑽％。各自 以如下所示方法將黏液作水萃取。 製作將來自洋蔥莖葉部黏液的粉末、及來自惠之葉身 邛黏液的粉末，以各種濃度使溶解於i〇%fcs_e，-MEM培 養基的溶液，調整為lxlG5細胞細心孔，接種於％孔 平盤各自添加於6小時前培養的巨嗤細胞系細胞株 RAW264 (購自理化學研究所生物來源中心）之培養系統 。添加1M、時後採取上清液，使用EUSA套組（Βί〇ι^_ 公司製）測量TNF_a量。結果顯示於第6圖。 如第6 @所示’添加來自衫之莖葉部之粉末時之 ^NF_a量’與添加來自紅葉身雜液之粉末時之TNF_a 里相比為極高，比活性亦顯示約100倍高。 ”本發明並未限定於上述實施形態，可以於請求項所示 犯圍内之各種變更’可適宜組合實施形態所揭示的技術手 段而獲得的實施形態亦包含於本發明之技術範圍。 産業上之利用可能性 本發明係利用自蕙之葉身部、或洋蔥之莖葉部萃取之 具有活作用的免疫賦活成分，可廣泛利用於醫藥品 2職品、動物飼育用飼料、畜産用飼料、養瘦用飼料 等之領域。 【圖式簡單説明】 第1圖中之(a)〜(b)係顯示經由與本發明之—實施形態 27 201132291 相關的免疫賦活劑之細胞激素生成促進效果的圖。 第2圖顯示經由與本發明之一實施形態相關的免疫賦 活劑之巨嗟細胞之吞食作用促進效果的圖。 第3圖中之(a)〜（c)係調查於與本發明之一實施形態相 關的免疫賦活劑達到細胞激素之生成促進效果之免疫賦活 成分之萃取部位的影響的圖。 第4圖為調查與本發明之一實施形態相關的免疫賦活 劑中免疫賦活成分之财熱性的圖。 第5圖為調查與本發明之一實施形態相關的免疫賦活 劑中達到細胞激素之生成促進效果的免疫賦活成分之萃取 方法之影響的圖。 第6圖為比較來自蔥之免疫賦活成分與來自洋蔥之免 疫賦活成分對細胞激素之生成促進效果的圖。 【主要元件符號說明】 無0 28In addition, the extract residue after the hot water extraction was checked for the residual mucus of the active ingredient, and after it was homogenized, it was filtered with a nylon mesh (manufactured by SANPLATEC 25 201132291) having a diameter of 1 mm, and the obtained filtrate was dried by lyophilization. A powder is obtained. The yield of the powder of hot water extraction (residue) was 2.79%. Preparation of water extract, hot water extract, and hot water extraction (residue) to dissolve the solution in 10% FCS-E'-MEM medium at various concentrations, adjust to lxlO5 cells / 200 μί / well, inoculate 96 wells The plates were each added to a culture system of a macrophage cell line RAW264 (purchased from the Institute of Biological Sources of the Institute of Physical Chemistry) cultured 6 hours ago. After the addition for 18 hours, the supernatant was taken, and the amount of TNF-a was measured using an ELISA kit (manufactured by BioLegend). The results are shown in Figure 5. As shown in Fig. 5, the amount of TNF-a added to the water extract is higher than that of the hot water extract and the hot water extract (residue), and the specific activity (required for the same activity) The necessary amount) is about 10 times higher. In the above 4., the mucus does not contain an immunostimulating component, and in the above 5., the immunostimulating component is rich in heat, and the activity of the hot water extract and the hot water extract residue is inferior to that of the water extract, and is considered to be poor. Components that are not related to activity are also extracted. Therefore, the present immunostimulating component can also be extracted by hot water extraction, but the obtained extract not only has different traits and flavors, but also has low activity (7: comparison between the immunostimulating component from onion and the immunostimulating component from onion) The immunostimulating action of the immunostimulating component is compared by the extracted plants. First, the mucus is extracted from the leaf body of the onion with the above 1. The yield of the obtained powder (water extract) (weight to the body part) is 0.32%. 26 201132291 and the stems and leaves of the onion are also The extraction was carried out in the same manner, and the yield (weight of the stem and leaf) of the obtained powder (water extract) was! (10)%. Each of the mucilage was subjected to water extraction as follows. A powder from the mucus of the onion stems and leaves, and a powder from the mucus of the leaves of the leaves of the leaves were prepared, and the solution dissolved in i〇%fcs_e, -MEM medium was adjusted to a fine hole of lxlG5 cells at various concentrations, and inoculated into the % well. The plates were each added to a culture system of the python cell line RAW264 (purchased from the Institute of Biological Sources of the Institute of Physical Chemistry) cultured 6 hours ago. After adding 1 M, the supernatant was taken, and the amount of TNF_a was measured using an EUSA kit (manufactured by Βί〇ι^_ company). The results are shown in Figure 6. As shown in the sixth @', the amount of ^NF_a when adding powder from the stems and leaves of the shirt is extremely high compared with the case of adding TNF_a from the powder of the red leaf body, and the specific activity is also about 100 times higher. The present invention is not limited to the above-described embodiments, and various embodiments that can be combined with the technical means disclosed in the embodiments can be included in the technical scope of the present invention. Advantages of the Invention The present invention utilizes an immunostimulating component which is active from the leaf body of the scorpion or the stem and leaf of the onion, and can be widely used in the pharmaceutical 2 product, the animal feeding feed, the livestock feed, Field of Feeding for Thinning, etc. [Brief Description of the Drawings] (a) to (b) in Fig. 1 show the effect of promoting cytokine production by the immunostimulating agent according to the present invention - Embodiment 27 201132291. Fig. 2 is a view showing the effect of promoting the swallowing action of the giant scorpion cells of the immunostimulating agent according to an embodiment of the present invention. (a) to (c) in Fig. 3 are investigated in accordance with the present invention. A diagram showing the influence of an immunostimulating agent according to an embodiment on an extraction site of an immunostimulating component that promotes the production of a cytokine. Fig. 4 is a view showing an embodiment of the present invention. Fig. 5 is a diagram for investigating the influence of an extraction method of an immunostimulating component that achieves a cytokine production-promoting effect in an immunostimulating agent according to an embodiment of the present invention. Fig. 6 is a graph comparing the effect of the immunostimulating component from onion and the immunostimulating component from onion on the production of cytokines. [Key element symbol description] None 0 28