201043962 六、發明說明： 【發明所屬之技術領域】 本發明係關於-種生物晶片，特別是關於_____ 合流體之微型磁流體混合器。 /tt4 【先前技術】 目前生物晶片之應用包含有試劑混合'樣品混合、里 相萃取、微量藥品配製、核酸及蛋白f分解等方面，如ς 流體混合n即為流體混合之應用。微流體混合器由於使用 樣品量少、製作成本低’適合應用作為辅助生物化學 之裝置，例如PCR分析或DNA雜交分析研究。然而，在 微流道内流體的雷諾數相纽，而錢體雷魏低的情況 下，流體傾向於分層流動，使不同之流體間產生明顯之分 界’而難以藉由紊流之效應混合二種以上之流體。因此， 近年來_有各錄越混合器乂卩姆，主要包含 混合器及被動式混合器兩大類。 工 習知被動核紐齡器之混合方法包含多孔式進 料法、三維折4料，料需藉由任何外力軸，僅需利 力所產生之自旋現象以進行混合，且在流體之 至_間之條件下’被動式微流體混合器可 ==混合效果。然而，由於利用被動式微流體混合 2=:_合高_之流體，以提昇混合效率，因 合^仍有侷限，且混合效率仍不如主動式微流體混 習知主動式微流體混合器則利用外力驅動，使流體在 201043962 微型結構巾產生不穩定之擾動而混合，包含以機械力方式 產生擾動、以微電極在局部進行介電泳力猶產生混洗流 場或是利用超音波震誠續料。然而，絲式微流體 混合器通常具有娜之微結構，導致製昂貴，晶片 设计亦較為困難，因此利用主動式微流體混合器進行流體 混合之成本較高。綜上所述’ f知微流體混合$仍無法在 簡單之微結構下，提昇低流速微流體之混合效率。 【發明內容】201043962 VI. INSTRUCTIONS OF THE INVENTION: TECHNICAL FIELD OF THE INVENTION The present invention relates to a biochip, and more particularly to a micro-magnetic fluid mixer for a _____ fluid. /tt4 [Prior Art] At present, the application of biochips includes reagent mixing 'sample mixing, phase extraction, micro drug preparation, nucleic acid and protein f decomposition, such as ς fluid mixing n is the application of fluid mixing. The microfluidic mixer is suitable for use as a device for assisting biochemistry, such as PCR analysis or DNA hybridization analysis, due to the small amount of sample used and low production cost. However, in the case where the Reynolds number of the fluid in the microchannel is relatively low, and the volume of the body is low, the fluid tends to stratify, causing a distinct boundary between the different fluids, and it is difficult to mix by the effect of turbulence. More than one type of fluid. Therefore, in recent years, there have been two types of mixers, including mixers and passive mixers. The mixing method of the passive nuclear aging device includes a porous feeding method, a three-dimensional folding material, and the material needs to be mixed by any external force shaft, which only needs the spin phenomenon generated by the force, and is in the fluid Under the condition of 'passive microfluidic mixer can == mixing effect. However, due to the use of passive microfluidics to mix 2 =: _ high _ fluid to improve mixing efficiency, there are still limitations due to the combination, and the mixing efficiency is still not as good as the active microfluidics. The active microfluidic mixer is driven by external force. The fluid is mixed in the instability of the 201043962 micro-structured towel, including the mechanical force to generate the disturbance, the microelectrode in the local dielectrophoretic force to produce the mixed flow field or the use of ultrasonic vibration. However, wire-type microfluidic mixers typically have a nanostructure that is expensive, and the wafer design is difficult, so the cost of fluid mixing using an active microfluidic mixer is high. In summary, the microfluidic mixing can still improve the mixing efficiency of low flow microfluids under a simple microstructure. [Summary of the Invention]
本發明係提供一種微型磁流體混合器，其發明目的係 用以解決習用微流體混合n無法提供在簡單的微結構下提 供良好之混合效率。 為達到前述發明目的，本發賴運用之技術手段及藉 由該技術手段所能達到之功效包含有： 曰 -種微型磁流體混合器，包含：—基座及—電磁鐵， 該基座設有-第―通道、—第二通道及—混合通道，該第 了通^及第二通道係交會連通於該混合通道，且該第一通 道、第二通道及混合通道分別具有-第-人口、—第二= 及料磁鐵設於該混合通道-侧，該電二 :、甬、貧蕻士及一第二端’該第一端及第二端係鄰近該混 。利用該電磁鐵所形成之磁場帶動磁流體產 中二動’再_該擾動將磁流體中的溶質均勻分散於溶劑 201043962 交流電使該電磁鐵產生磁極變化’帶動該 【實施方式】 A讓本發明之上述及其他目的、特徵及優點能更明顯 易懂，下文特舉本發明之較佳實施例，並配合所附圖式， 作詳細說明如下： ❼照第1圖所示’本發明之微型磁流體混合器包含 #基座卜-電磁鐵2及—電源供應器3。該基座i設有一 弟通道1卜-第二通道12及一混合通道ι3，各該通道 ' :13係於該基座1内水平延伸，且該第—通道U 及第-通道12係父會連通於該混合通道13，其中該第— 〃第—通道12及混合通道13之管徑較佳為150# m «亥第通遏n、第二通道u及混合通道η未相互連 通之一端係朝該基座1之頂面延伸且貫穿該基座i之了頁 面，分別形成-第-人口 m、—第二人口 121及一出口 2 _示’該基座丨較佳係由—底板14及蓋板 且δ $成，且該底板14之表面具有相連通之數個凹槽， =蓋^ 15則具有㈣通孔1各該通孔之位置係對應於 凹槽之—端。藉此’將該底板14與該蓋板15疊合， :可ί該底板14與蓋板15之凹槽及通孔形成該第-通道 之頂面7道12及混合通道13，且各魏錄於蓋板15 側即分別形成該第—入口⑴、第二入口 i2i -绫L:3!。該電磁鐵2係設於該混合通道13 -侧，且由 圈〜於—鐵心之外周面所構成。該電磁鐵2具有- 201043962 弟-巧及-第二端22 ’該第一端21及第二端22係鄰 近該混合通道u，例如，該第—端21及第二端22虚妒 合通道13之距離較佳為1贿。因此，當該電磁鐵;、通^ =:端21及第二端22可形成磁極，產生磁場吸引 . «源供應器3係具有-第—電極31及—第二電極 32，該第-電極31及第二電極％係透過導線連接該電磁 鐵2之線圈，且該第一電極31與第二電極32間之電位差 係、由—最錄至—最小值循環變化，其中該最大值為正值 而該最小值為貞值，以提供—交流電魏電磁鐵2。 其中，該第一入口 1H供導入溶劑，該第二入口 ΐ2ι 供導入含溶質之磁流體’該出口 131供導出混合之溶液。 例如’該溶劑可選自去離子水，該溶質可選自藥物，該磁 流體可選自鐵磁流體〔ferr0fluid〕。鐵磁流體是一種磁場 存在時強烈極化的液體，由懸浮於載流體當中奈米數量級 ◎ _磁微粒組成，載趙通常是有機溶舰水，鐵磁微粒 係由表面活性劑包覆以防止其因凡得瓦力〔van der Wads f〇rce〕及磁力作用而發生凝聚。鐵磁微粒的典型大小為忉 2米，在這個尺度下，熱攪動效應可以使該鐵磁微粒在载 々II·體中被均一地分散開，從而使鐵磁微粒可賦予一整體之 磁性反應於該鐵磁流體，使該鐵磁流體表現順磁性。使用 本發明之微型磁流體混合器時，將該電源供應器3之電源 碭啟使该電磁鐵2之二端形成磁極。再將該溶劑與含有 溶質之礤流體分別由該第一入口 111及第二入口 121導入 該第通道11及第二通道12，且該溶劑及含有溶質之磁 201043962 通道11、12被推擠流至該混合通道13。 /、中’ * —有溶質之磁流體通過該控制區132時， ίΓ亍则磁鐵2所產生之磁場影響而 進仃奴轉m而帶動該溶劑 合，使該溶質均勻分散於舰 體混 收集混合完成之溶液。 最後再由该出口 131 ，發明之微型磁流體混合器較 造：請參照第3 S国π - Γ ^ ^ ^ ，、第至5圖所不，該底板 形成-光阻膜4，利用紫外光穿過 塗佈 4，使該遮θ安# 遮罩5…、射该光阻膜 使4罩5之圖案轉移至該光阻膜4 紫=射之位置逐漸軟化後，以藥劑洗 =: 4而路出該底板14之部分表面，再膜 〔BOE〕蝕刻該底板14去 ，、、衝氧化物蝕刻液 ㈣ί 光阻膜4覆蓋之位置，以便在 4。於W板述之凹槽’並隨後移除該光阻膜 2於;= 形成該數個穿孔，再將該蓋板15 m反η形成該凹槽之表面，並利用融熔接合 :最r〕f_固定於底板14上，以形成 二，後，將該電磁體2固定於該基板！之混合通 ^ ^如此即凡成該微型磁流體混合器。 、本發明之微型磁流體混合器之混合效率可利用下列 it檢驗斜請參照第6圖所示，分別將去離子水及鐵 n人二㈣，該二針筒分別透過-軟管連接該第-…1及第二入口121，將該二針筒架設於針;幫工， :=筒!浦控制該去離子水注入該第-通道二 入弟—通道12之流速。開啟該電源供應器3，並控 201043962 制其提供之電壓介於o S 2·5伏特〔V〕間，電流介於〇 至3.3安培〔Α〕間，因此電力介於〇至8 25瓦特〔w〕 間。再利用CCD攝影機61拍攝該混合通道13之一控制區 132’該CCD攝影機61係連接一電腦62，利用電腦犯放 大顯示該混合區132之去離子水及鐵磁流體的混合狀況。 其中該控制區132係形成於該混合通道13鄰近該電磁鐵2 之第一端21之位置，使磁極產生之磁場在該控制區132 與鐵磁流體作用，以供利用CCD攝影機61在該控制區132 偵測該溶劑與溶質之混合狀態。 請參照附件1所示，在電壓L0V、電流17A的條件 下〔電力1.7W〕，該溶液的混合比率為95.2% ;在電壓 2.0V、電流2.9A的條件下〔電力5.8W〕，該溶液的混合 比率為97.9% ;在電壓2·5ν、電流3 3A的條件下〔電力 8.25W〕，該溶液的混合比率為99 〇%。請參照附件2所 不，控制該交流電之頻率分別為45Ηζ、75Ηζ& 1〇〇Ηζ〔分 別如附件2之(a)、（b)及(c)所示〕，在電壓2.0V、電流2.9Α 條件下〔電力5.8W〕，該溶液的混合狀況無明顯差異。 、本毛明之从型磁流體混合器利用該電磁鐵2所形成之 磁場，並利用該交流電使該電磁鐵2產生之磁極變化，帶 動該磁流體產生擾動，再利用該擾動將磁流體中的溶質均 勻分散於溶劑中。因此，欲進行流體之混合時，僅需將該 溶劑及含有溶質之磁流體分別導入該第一通道n及第二 通道12，使其流過該混合通道13之控制區132即可完成 混合，且該微型磁流體混合器結構簡單，混合效率良好， 了應用之領域更加廣泛。 201043962 雖然本發明已利用上述較佳實施例揭示，然其並非用 以限定本發明，任何熟習此技藝者在不脫離本發明之精神 和範圍之内，相對上述實施例進行各種更動與修改仍屬本 發明所保護之技術齡’因此本發明之保護範圍當視後附 之申請專利範圍所界定者為準。 【圖式簡單說明】 第1圖：本發明較佳實施例之微型磁流體混合器之組合 俯視圖。 第2圖：本發明較佳實施例之微型磁流體混合器之分解 立體圖。 第3圖.本發明較佳貫施例之微型磁流體混合器之紫外 光照射光阻膜示意圖。 第4圖：本發明較佳實施例之微型磁流體混合器之底板 形成凹槽示意圖。 第5圖.本發明較佳實施例之微型磁流體混合器之蓋板 及底板接合示意圖。 第6圖：本發明較佳實施例之微型磁流體混合器之電腦 债測混合狀態示意圖。 附件1 :本發明較佳實施例之微型磁流體混合器之溶液 混合比率與磁場強度關係圖。 附件2·本發明較佳實施例之微型磁流體混合器在各頻 率下之溶液混合狀態。 【主要元件符號說明】 〔本發明〕 —10 — 201043962 1 基座 11 111 第一入口 12 121 第二入口 13 131 出口 132 14 底板 15 2 電磁鐵 21 22 第二端 3 電源供應益 31 32 第二電極 4 光阻膜 5 61 攝影機 62 第一通道 第二通道 混合通道 控制區 蓋板 第一端 第一電極 遮罩 電腦SUMMARY OF THE INVENTION The present invention provides a miniature magnetic fluid mixer whose object is to solve the problem of conventional microfluidic mixing that does not provide good mixing efficiency under a simple microstructure. In order to achieve the foregoing object, the technical means and the effects that can be achieved by the technical means include: a micro-magnetic fluid mixer comprising: a base and an electromagnet, the base is provided a first channel, a second channel, and a second mixing channel, wherein the first channel, the second channel, and the second channel are connected to the mixing channel, and the first channel, the second channel, and the hybrid channel respectively have a -first population , the second = and the material magnet are disposed on the side of the mixing channel, the electric two: 甬, the poor gentleman and a second end 'the first end and the second end are adjacent to the mixing. Using the magnetic field formed by the electromagnet to drive the magnetic fluid to produce two-action 're-distribution, the solute in the magnetic fluid is uniformly dispersed in the solvent 201043962, and the alternating current causes the electromagnet to generate a magnetic pole change'. [Embodiment] A The above and other objects, features and advantages of the present invention will become more apparent and understood. The magnetic fluid mixer includes a #基卜-electromagnet 2 and a power supply 3. The pedestal i is provided with a dipole channel 1 - a second channel 12 and a mixing channel ι 3 , each of the channels ' : 13 is horizontally extended in the pedestal 1 , and the first channel U and the first channel 12 are fathers It is connected to the mixing channel 13 , wherein the diameter of the first channel 12 and the mixing channel 13 is preferably 150# m «the first channel u, the second channel u and the mixed channel η are not connected to each other. a page extending toward the top surface of the susceptor 1 and extending through the pedestal i, forming a -first population m, a second population 121, and an outlet 2 - indicating that the pedestal 丨 is preferably a bottom plate 14 and the cover plate and δ $ is formed, and the surface of the bottom plate 14 has a plurality of grooves communicating with each other, and the cover member 15 has (four) through holes 1 each of which corresponds to the end of the groove. Thereby, the bottom plate 14 is overlapped with the cover plate 15, and the groove and the through hole of the bottom plate 14 and the cover plate 15 form the top surface 7 of the first passage and the mixing passage 13 and the mixing passage 13 The first inlet (1) and the second inlet i2i - 绫L: 3! are formed on the side of the cover plate 15, respectively. The electromagnet 2 is disposed on the side of the mixing passage 13 - and is constituted by a circumference of the outer circumference of the core. The electromagnet 2 has - 201043962 - the second end 22 - the first end 21 and the second end 22 are adjacent to the mixing channel u, for example, the first end 21 and the second end 22 are falsely coupled The distance of 13 is preferably 1 bribe. Therefore, when the electromagnet; the pass terminal 21 and the second end 22 can form a magnetic pole, magnetic field attraction is generated. The source supplier 3 has a -electrode 31 and a second electrode 32, and the first electrode 31 and the second electrode % are connected to the coil of the electromagnet 2 through a wire, and the potential difference between the first electrode 31 and the second electrode 32 is cyclically changed from - most recorded to - minimum, wherein the maximum value is positive The value is the 贞 value to provide - the alternating current electromagnet 2. Wherein, the first inlet 1H is for introducing a solvent, and the second inlet ΐ2 is for introducing a solute-containing magnetic fluid 'the outlet 131 for deriving the mixed solution. For example, the solvent may be selected from the group consisting of deionized water, and the solute may be selected from the group consisting of a drug, and the magnetic fluid may be selected from a ferrofluid. A ferrofluid is a liquid that is strongly polarized in the presence of a magnetic field. It consists of nanometers of magnitude ◎ _ magnetic particles suspended in a carrier fluid. The carrier is usually organically dissolved in water, and the ferromagnetic particles are coated with a surfactant to prevent it. It is agglomerated by van der Wads f〇rce and magnetic action. The typical size of ferromagnetic particles is 忉2 m. At this scale, the thermal agitation effect allows the ferromagnetic particles to be uniformly dispersed in the yttrium II, so that the ferromagnetic particles can impart a magnetic reaction as a whole. The ferrofluid causes the ferrofluid to exhibit paramagnetism. When the micro magnetic fluid mixer of the present invention is used, the power supply of the power supply 3 is turned on to form the magnetic poles at both ends of the electromagnet 2. The solvent and the solute containing solute are respectively introduced into the first channel 11 and the second channel 12 from the first inlet 111 and the second inlet 121, and the solvent and the solute-containing magnetic 201043962 channels 11, 12 are pushed out. To the mixing channel 13. /, medium '*' - when the solute magnetic fluid passes through the control zone 132, the magnetic field generated by the magnet 2 affects the nucleus and drives the solvent to make the solute uniformly dispersed in the hull. Mix the finished solution. Finally, the outlet 131, the invention of the micro magnetic fluid mixer is more: please refer to the 3rd S country π - Γ ^ ^ ^, and the 5th to the fifth, the bottom plate forms a photoresist film 4, using ultraviolet light After passing through the coating 4, the mask is placed on the mask 5, and the photoresist film is irradiated to transfer the pattern of the 4 cover 5 to the photoresist film 4. The position of the purple film is gradually softened, and then washed with a drug = 4 And a part of the surface of the bottom plate 14 is removed, and the bottom plate 14 is etched by the film (BOE), and the oxide etching liquid (4) is etched by the photoresist film 4 so as to be at 4. The groove of the W plate is described and then the photoresist film 2 is removed; = the plurality of perforations are formed, and the cover plate 15 m is reversely n-formed to form the surface of the groove, and the fusion bonding is performed: ] f_ is fixed on the bottom plate 14 to form two, and then the electromagnet 2 is fixed to the substrate! The hybrid pass ^ ^ is thus the micro-magnetic fluid mixer. The mixing efficiency of the micro magnetic fluid mixer of the present invention can be measured by the following it. Please refer to Fig. 6 for deionized water and iron n (four), respectively. -...1 and the second inlet 121, the two needle cylinders are mounted on the needle; the assistant, the == cylinder; the pump controls the flow rate of the deionized water into the first passage - the second passage - the passage 12. Turn on the power supply 3, and control the voltage supplied by 201043962 to be between o S 2·5 volts [V], the current is between 〇 and 3.3 amps, so the power is between 〇 and 8 25 watts. w). Then, the CCD camera 61 is used to take a control area 132' of the mixing channel 13. The CCD camera 61 is connected to a computer 62 for displaying the mixing condition of the deionized water and the ferrofluid in the mixing zone 132 by the computer. The control region 132 is formed at a position adjacent to the first end 21 of the electromagnet 2, so that a magnetic field generated by the magnetic pole acts on the control region 132 and the ferrofluid for use in the control by the CCD camera 61. Zone 132 detects the mixing of the solvent and the solute. Please refer to the attached item 1. Under the conditions of voltage L0V and current 17A [electricity 1.7W], the mixing ratio of the solution is 95.2%; under the condition of voltage 2.0V and current 2.9A [electricity 5.8W], the solution The mixing ratio was 97.9%; at a voltage of 2·5 ν and a current of 3 3 A (electricity 8.25 W), the mixing ratio of the solution was 99 〇%. Please refer to Appendix 2 for the frequency of controlling the AC power of 45 Ηζ, 75 Ηζ & 1 〇〇Ηζ [as shown in (a), (b) and (c) of Annex 2 respectively) at a voltage of 2.0 V and a current of 2.9. Α Under the condition [electric power 5.8W], there is no significant difference in the mixing condition of the solution. The magnetic fluid mixer of the present invention uses the magnetic field formed by the electromagnet 2, and uses the alternating current to change the magnetic pole generated by the electromagnet 2, causing the magnetic fluid to be disturbed, and then using the disturbance to move the magnetic fluid. The solute is uniformly dispersed in the solvent. Therefore, when mixing the fluid, the solvent and the solute-containing magnetic fluid need to be separately introduced into the first passage n and the second passage 12 to flow through the control zone 132 of the mixing passage 13 to complete the mixing. Moreover, the micro magnetic fluid mixer has a simple structure, good mixing efficiency, and a wider application field. Although the present invention has been disclosed in the above-described preferred embodiments, it is not intended to limit the invention, and various modifications and changes to the above embodiments are possible without departing from the spirit and scope of the invention. The technical scope of the invention is therefore to be determined by the scope of the appended claims. BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a plan view showing a combination of a micro magnetic fluid mixer of a preferred embodiment of the present invention. Fig. 2 is an exploded perspective view of the micro magnetic fluid mixer of the preferred embodiment of the present invention. Fig. 3 is a schematic view showing a UV light-shielding film of a micro-magnetic fluid mixer according to a preferred embodiment of the present invention. Fig. 4 is a schematic view showing the formation of a groove in the bottom plate of the micro magnetic fluid mixer of the preferred embodiment of the present invention. Fig. 5 is a schematic view showing the bonding of the cover and the bottom plate of the micro magnetic fluid mixer of the preferred embodiment of the present invention. Figure 6 is a schematic view showing the state of the computer debt measurement mixing state of the micro magnetic fluid mixer of the preferred embodiment of the present invention. Annex 1 is a graph showing the relationship between the solution mixing ratio and the magnetic field strength of the micro magnetic fluid mixer of the preferred embodiment of the present invention. Annex 2. The solution mixing state of the micro magnetic fluid mixer of the preferred embodiment of the present invention at various frequencies. [Description of main components] [Invention] - 10 - 201043962 1 Base 11 111 First inlet 12 121 Second inlet 13 131 Exit 132 14 Base plate 15 2 Electromagnet 21 22 Second end 3 Power supply benefit 31 32 Second Electrode 4 photoresist film 5 61 camera 62 first channel second channel mixing channel control zone cover first end first electrode mask computer
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