201013181 九、發明說明： 【發明所屬之技術領域】 本發明係關於一種基板，特別是一種有關於生化檢測與免疫檢測所使用之流 體檢測試片所使用的基板。 【先前技術】 以流體檢測試片進行生化檢測與免疫檢測的習知技術中，流體檢測試片在其 基板或底材上設計有流道或微流道結構，而因流道周圍並非吸水材質，且待測 Φ流體多為含有如蛋白質或是醣類等黏滯度高之組成物，所以當待測流體流過 後，會在流道上殘留’使得待測流艎無法完全反應，如此一來，不僅造成待測 流體的浪費，更可能造成最終測試結果的誤差。 此外’習知技術的流體檢測試片在流趙傳送方面，可設計有微流道結構，並 係利用微流道結構產生的毛細現象，將流體經過流道被動傳送至反應偵測區 域；另一種方式則是在注入待測流體時即利用加壓等方式，給予流體一驅動力， 使得流體可主動通過流道，到達反應偵測區域。但是無論是上述任一種方式， 待測流體注入流道後常常產生大小不一的氣泡使得流道阻塞，造成實際測量上 之誤差，甚至致使測試失敗。 ® 驗’習知技術的檢測試#，在製作上多使用模鑄或射出成型或壓印(imprint) 的方式在基板上做減道或微流道結構，所以必須使用聚乙稀（pE)、聚氣乙 稀（PVO絲丙烯（PP)等價格較高之歸縣物作級狂模具耗損較快， 進而造成試片之總體成本的提高。 【發明内容】 為克服上述之缺點，本發明提供一種流體檢測試片之基板，基板上表面向下 贱至少-流道’此流道包含依序連接之第—流體區、第二流體區與第三流體 區’第-魏區雜_之注人。此基板之特徵在於第二碰區與第三流體區 之底部形成有硝 1 纖維層，此石肖化纖維層包含有中_狀構型，崎道中第二 5 201013181 流體區是供流體傳送之用，第三流艘區是供流體反應之用，且第二流體區的確 化纖維層平均厚度不大於第三流體區硝化纖維層厚度。 因此，本發明之主要目的，係提供一種流體檢測試片之基板，因具有可吸水 的硝化纖維層，故可避免流道之待測流體殘留。 本發明之另一目的，係提供一種流體檢測試片之基板，其具有可吸水的硝化 纖維層，由於單位艎積的硝化纖維吸水量係為定值，故可經由設定基板上硝化 纖維層的體積，而提供流體的定量檢測。 本發明之又一目的，係提供一種流體檢測試片之基板，具有中空網狀構型的 〇硝化纖維層，由於流體流經中空網狀構型時，流體中的氣泡會被破壞，故可消 彌較大的氣泡，避免微流道技術中氣泡阻塞流道的狀況發生，進而影響定量分 析結果。 ’ 【實施方式】 由於本發明係揭露一種流體檢測試片之基板，其中所利用物理、化學原理及 溶液塗布技術，已為相關技術領域具有通常知識者所能明瞭，故以下文中之說 明，不再作完整描述。同時，以下文中所對照之圖式，係表達與本發明特徵有 關之示意，並未亦不需要依據實際情形完整繪製，合先敘明。 Ο 如第1A圖所示，為本發明第一較佳實施例之流體檢測試片的基板示意圖。 流體檢測試片的基板1自上表面10向下凹設有流道u，流道u包含有依序連 接的第一流體區111、第二流體區112與第三流體區113 ^第一流體區U1係供 流體之注入，流體在注入第一流體區111後，經由第二流體區112的傳送，到 達第三流體區113，流體中的待測成份在第三流體區113進行反應。又在較佳 的實施狀態中，基板1為生物相容(biocompatible)材料。 請參考第1B圖’為第1A圖中沿AA連線之剖面圖。基板i的特徵在於， 在第二流體區112與第三流體區113之底部均形成有硝化纖維層U21與1131 ’ 而硝化纖維層1121與1131的構型為中空網狀，此種具有多孔性的中空網狀結 構，可以吸收由第一流體區111流入的流體，使得流體令的待測成份與存在於 6 201013181 硝化纖維層1131中的試劑進行反應。由於硝化纖維層1121與1131係為可吸水 材質，故可避免流體殘留於流道11中，且由於流體流經中空網狀構型的硝化纖 維層1121與1131時，流體中的氣泡會被破壞，故可避免氣泡阻塞流道11。此 外，第二流體區112的硝化纖維層1121平均厚度Da不大於第三流體區U3硝 化纖維層1131厚度Db，亦即Da會小於或等於Db。此外，為降低所需生物檢 體之體積，第二流體區112與第三流體區113的寬度Wa與Wb (如第1A圖所 示）較佳至少為0.3mm。 在製作上，硝化纖維層1121與1131的形成方式，係將硝化纖維粉末 ©(nitrocellulose powder)與含有酯類（ester)和酮類（ketone)的有機溶劑混人 後形成一硝化纖維溶液；再將形成後的硝化纖維溶液，澆注（casting)於第二流 體區112與第三流體區113的底部，經乾燥後，於第二流體區112底部則會形 成硝化纖維層1121 ’而於第三流體區ι13的底部則形成硝化纖維層1131。為達 較佳的澆注效果’流道11之表面粗糙度（如值）以介於3微米至5〇微米之間 為佳。 硝化纖維溶液乾燥後形成具有中空網狀構型的硝化纖維層，為了調整較佳的 中空網狀構型，本發明的硝化纖維溶液中，硝化纖維粉末與含有酯類和嗣類的 有機溶劑混合的較佳體積比例為i : 9。由於單位體積的硝化纖維吸水量係為定 Ο值，故可由欲吸收之待測流體的體積推算出對應的硝化纖維溶液的體積，之後 再行澆注。如此可以固定檢測所需液體之體積量，並適用於微量檢測。 上述之第-較佳實施例係為具有三個流體區域的檢測試片基板，而根據本發 明=精神’尚可在流道中加設第四流體區，以供儲存流道中多餘之流體。以下 將就本發明之第二實施例具有四個流體區的檢測試片基板進行描述。 請參考第2A ® ’為本發明第二實施例之流體檢測試片的基板示意圖。基 =自上表面20向下凹設有流道2卜流道21包含有依序連接的第一流體區 味、第二流體區212、第三流體213 1與第四流體區214。第一流體區2ιι係 供流體之注入’流體在注入第一流體區211後，經由第二流趙區2D的傳送， 7 201013181 到達第三紐區2U，流體巾崎戦份在第三流體區2i3反應。 ❹ ❿ 一 4參考第2B圖’為第2八圖沿从連線之剖面圖。在第二流體區犯與第 二流體區μ之底部均形成有磺化纖維層簡與，且第二流體區加的瑞 化纖維層2121平均厚度Dc等於第三流體區犯硝化纖維層迎厚度別。第 流雜區214之底部與第一及第三流體區212、213相同，亦形成有頌化纖維層 41且亦為中工網狀構型’以供多餘流趙之貯存。而第四流體區叫底部之 肖化纖維層2141 ’在製作上，與第二及第三賴區212、213底部之雜纖維層 2121與2131相同’均以硝化纖維溶液經洗注於第二流體區212、第三流體區犯 與第四流體區214之底部再經乾燥後所形成。 此外’本實施例中第-流趙區、第二流體區、第三流體區之構造、尺寸與相 互連接關係、基板之較佳材料、表面_度、基板中硝化纖維層之構型、形成 方式、使狀硝化_溶液之成份與姉_，均與第—實關_， 不再重複贅述。 以上所述僅為本發明較佳實施例而已，並非用以限定本發明申請專利權化 同時以上的贿騎熟之本技術賴之專門人均，瞭與實施，因此其他未 脫離本發明示之精神下所絲的等贱變雜飾，均耻含於下述 專利範圍。 β 【圖式簡單說明】 第1Α圖，為本發明第一實施例流體檢測試片的基板之示意圖。 第1Β圖’為本發明第―實施例流體檢測試片的基板剖面之示意圖。 第2Α圖，為本發明第二實施例流體檢測試片的基板之示意圖。 第2Β圖’為本發明第二實施例流體檢測執片的基板剖面之示意圖。 【主要元件符號說明】 1 ' 2 10、20 基板 上表面 201013181201013181 IX. Description of the Invention: [Technical Field] The present invention relates to a substrate, and more particularly to a substrate for use in a fluid detecting test piece for use in biochemical detection and immunoassay. [Prior Art] In the conventional technique of performing biochemical detection and immunodetection using a fluid detecting test piece, the fluid detecting test piece is designed with a flow path or a micro flow path structure on the substrate or the substrate, and the surrounding material is not a water absorbing material. And the Φ fluid to be tested mostly contains a composition with high viscosity such as protein or sugar, so when the fluid to be tested flows, it will remain on the flow channel, so that the flow to be tested cannot be completely reacted, thus Not only causes waste of the fluid to be tested, but is more likely to cause errors in the final test results. In addition, the fluid detection test piece of the prior art can be designed with a micro-flow channel structure, and utilizes the capillary phenomenon generated by the micro-channel structure to passively transfer the fluid through the flow path to the reaction detection area; One way is to give a driving force to the fluid when the fluid to be tested is injected, that is, by means of pressurization, etc., so that the fluid can actively pass through the flow path to reach the reaction detecting area. However, in either of the above manners, the fluid to be tested is often injected into the flow channel to cause bubbles of different sizes to block the flow path, causing an error in actual measurement and even causing the test to fail. ® Inspection of the 'Knowledge Technology', the use of die casting or injection molding or imprinting on the substrate to make the reduction or micro-flow structure on the substrate, so the use of polyethylene (pE) must be used The gas smelting (PVO propylene (PP), etc., which has a higher price, is more expensive, and the overall cost of the test piece is increased. [Invention] To overcome the above disadvantages, the present invention Providing a substrate for a fluid detecting test piece, wherein the upper surface of the substrate is downwardly 贱 at least - the flow path 'this flow path includes the first fluid region, the second fluid region and the third fluid region 'the first - Wei region The substrate is characterized in that a nano-fiber layer is formed at the bottom of the second collision zone and the third fluid zone, and the stone-shadowed fiber layer comprises a medium-shaped configuration, and the second 5 in the kadaodao 201013181 fluid zone is a fluid supply layer. For the purpose of transmission, the third flow zone is for fluid reaction, and the average thickness of the confirmed fiber layer of the second fluid zone is not greater than the thickness of the nitrocellulose layer of the third fluid zone. Therefore, the main object of the present invention is to provide a flow. The substrate of the test strip, There is a layer of nitrocellulose which can absorb water, so that the fluid to be tested in the flow channel can be avoided. Another object of the present invention is to provide a substrate for a fluid detecting test piece which has a water absorbing nitrocellulose layer due to unit hoarding. The nitrocellulose water absorption amount is a constant value, so that the quantitative detection of the fluid can be provided by setting the volume of the nitrocellulose layer on the substrate. Another object of the present invention is to provide a substrate for a fluid detecting test piece having a hollow network structure The type of niobium-nitrocellulose layer, because the fluid flows through the hollow network configuration, the bubbles in the fluid will be destroyed, so that larger bubbles can be eliminated, and the condition of the bubble blocking channel in the micro-channel technology is avoided. The present invention relates to a substrate for a fluid detecting test piece, in which the physical, chemical principles and solution coating techniques utilized are well known to those skilled in the relevant art, so The description herein is not fully described. At the same time, the drawings in the following text are indicative of the features related to the features of the present invention. It is not necessary to draw a complete picture according to the actual situation, which is described in detail. Ο As shown in FIG. 1A, it is a schematic diagram of a substrate of a fluid detecting test piece according to a first preferred embodiment of the present invention. The substrate 1 of the fluid detecting test piece is self-contained. The upper surface 10 is recessed downwardly with a flow path u. The flow path u includes a first fluid zone 111, a second fluid zone 112 and a third fluid zone 113. The first fluid zone U1 is used for fluid injection. After the fluid is injected into the first fluid zone 111, it is transferred to the third fluid zone 113 via the second fluid zone 112, and the component to be tested in the fluid reacts in the third fluid zone 113. In a preferred embodiment, The substrate 1 is a biocompatible material. Please refer to FIG. 1B' for a cross-sectional view along line AA in FIG. 1A. The substrate i is characterized by being at the bottom of the second fluid region 112 and the third fluid region 113. The nitrocellulose layers U21 and 1131' are formed, and the nitrocellulose layers 1121 and 1131 are configured in a hollow network. The porous hollow network structure can absorb the fluid flowing in from the first fluid region 111, so that the fluid The ingredients to be tested and existed on 6 201013181 The reagent in the nitrocellulose layer 1131 is reacted. Since the nitrocellulose layers 1121 and 1131 are water absorbing materials, fluid can be prevented from remaining in the flow path 11, and bubbles in the fluid are destroyed as the fluid flows through the nitrocellulose layers 1121 and 1131 of the hollow network configuration. Therefore, it is possible to prevent the bubble from blocking the flow path 11. Further, the average thickness Da of the nitrocellulose layer 1121 of the second fluid region 112 is not greater than the thickness Db of the nitrocellulose layer 1131 of the third fluid region U3, that is, Da may be less than or equal to Db. Further, in order to reduce the volume of the desired biological specimen, the widths Wa and Wb of the second fluid region 112 and the third fluid region 113 (as shown in Fig. 1A) are preferably at least 0.3 mm. In the production, the nitrocellulose layers 1121 and 1131 are formed by mixing a nitrocellulose powder with an organic solvent containing esters and ketones to form a nitrocellulose solution; The formed nitrocellulose solution is cast in the bottom of the second fluid zone 112 and the third fluid zone 113. After drying, a nitrocellulose layer 1121' is formed at the bottom of the second fluid zone 112. A nitrocellulose layer 1131 is formed at the bottom of the fluid zone ι13. For better casting effect, the surface roughness (e.g., value) of the flow path 11 is preferably between 3 micrometers and 5 micrometers. After the nitrocellulose solution is dried, a nitrocellulose layer having a hollow network configuration is formed. In order to adjust a preferred hollow network configuration, the nitrocellulose powder of the nitrocellulose solution of the present invention is mixed with an organic solvent containing an ester and a hydrazine. The preferred volume ratio is i: 9. Since the water absorption per unit volume of the nitrocellulose is a constant value, the volume of the corresponding nitrocellulose solution can be derived from the volume of the fluid to be absorbed, and then cast. This makes it possible to fix the volume of the liquid required for detection and to be suitable for micro-testing. The above-described preferred embodiment is a test strip substrate having three fluid regions, and according to the present invention, a fourth fluid region may be added to the flow path for storing excess fluid in the flow path. Next, a test strip substrate having four fluid regions according to a second embodiment of the present invention will be described. Please refer to FIG. 2A ' ' for a substrate diagram of a fluid detecting test piece according to a second embodiment of the present invention. Base = a flow path 2 recessed from the upper surface 20. The flow path 21 includes a first fluid zone, a second fluid zone 212, a third fluid 213 1 and a fourth fluid zone 214 which are sequentially connected. The first fluid zone 2 ιι is for the injection of fluid. After the fluid is injected into the first fluid zone 211, the second fluid zone 2D is transferred, 7 201013181 reaches the third zone 2U, and the fluid towel is in the third fluid zone. 2i3 reaction. ❹ ❿ 4 Refer to FIG. 2B' as a cross-sectional view of the connecting line in FIG. In the second fluid zone, a sulfonated fiber layer is formed at the bottom of the second fluid zone μ, and the average thickness Dc of the varnished fiber layer 2121 added in the second fluid zone is equal to the thickness of the third fluid zone nitrating fiber layer. do not. The bottom of the first flow region 214 is identical to the first and third fluid regions 212, 213, and is also formed with a strontium fiber layer 41 and also a medium mesh structure for storage of excess flow. The fourth fluid zone, called the bottom of the fiber layer 2141', is produced in the same manner as the second and third zones 212, 213 at the bottom of the fiber layers 2121 and 2131, and is washed with the nitrocellulose solution in the second The fluid zone 212, the third fluid zone, and the bottom of the fourth fluid zone 214 are formed by drying. In addition, in the present embodiment, the structure, the size and the interconnection relationship of the first-flow region, the second fluid region, and the third fluid region, the preferred material of the substrate, the surface degree, the configuration of the nitrocellulose layer in the substrate, and the formation The method, the content of the nitrification _ solution and the 姊 _, both with the first - actual _, will not repeat. The above description is only for the preferred embodiment of the present invention, and is not intended to limit the patent right of the present invention. The ambiguous miscellaneous ornaments under the silk are all included in the scope of the following patents. β [Simplified description of the drawings] Fig. 1 is a schematic view showing a substrate of a fluid detecting test piece according to a first embodiment of the present invention. Fig. 1 is a schematic view showing a cross section of a substrate of a fluid detecting test piece according to a first embodiment of the present invention. Fig. 2 is a schematic view showing a substrate of a fluid detecting test piece according to a second embodiment of the present invention. Fig. 2 is a schematic view showing a cross section of a substrate of a fluid detecting plate according to a second embodiment of the present invention. [Main component symbol description] 1 ' 2 10, 20 substrate upper surface 201013181
流道 11 ' 21 第一流體區 111 ' 211 第二流體區 112'212 第三流體區 113 > 213 第四流體區 214 硝化纖維層 1121 ' 13 ' 2121 ' 2131 > 2141 硝化纖維層平均厚度 Da 硝化纖維層厚度 Db 硝化纖維層平均厚度 Dc 硝化纖維層厚度 Dd 第二流體區的寬度 Wa 第三流體區的寬度 WbFlow path 11 ' 21 first fluid zone 111 ' 211 second fluid zone 112 '212 third fluid zone 113 > 213 fourth fluid zone 214 nitrocellulose layer 1121 ' 13 ' 2121 ' 2131 > 2141 average thickness of nitrocellulose layer Da nitrocellulose layer thickness Db nitrocellulose layer average thickness Dc nitrocellulose layer thickness Dd width of the second fluid zone Wa width of the third fluid zone Wb
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