TWI388825B - Substrate - Google Patents

Description

Substrate for fluid detection test piece

The present invention relates to a substrate, and more particularly to a substrate for use in a fluid test strip for use in biochemical detection and immunoassays.

In the conventional technique of performing biochemical detection and immunodetection using a fluid detecting test piece, the fluid detecting test piece is designed with a flow path or a micro flow path structure on the substrate or the substrate, and the surrounding of the flow path is not a water absorbing material, and is to be tested. The fluid is mostly composed of a highly viscous composition such as protein or saccharide. Therefore, when the fluid to be tested flows, it will remain on the flow path, so that the fluid to be tested cannot be completely reacted, thus not only causing the fluid to be tested. The waste is more likely to cause errors in the final test results.

In addition, the fluid detecting test piece of the prior art can be designed with a micro-flow channel structure in fluid transfer, and utilizes the capillary phenomenon generated by the micro-flow path structure to passively transfer the fluid through the flow path to the reaction detecting area; The method is to apply a driving force to the fluid when the fluid to be tested is injected, that is, using a pressure or the like, so that the fluid can actively pass through the flow path to reach the reaction detecting area. However, in either of the above manners, after the fluid to be tested is injected into the flow channel, bubbles of different sizes are often generated to block the flow path, causing an error in actual measurement, and even causing the test to fail.

Finally, the test strips of the prior art use a die casting or injection molding or imprinting method to make a flow path or a micro flow path structure on the substrate, so polyethylene (PE) must be used. High-priced plastic polymers such as polyvinyl chloride (PVC) or polypropylene (PP) are used as materials and the molds are worn faster, which in turn leads to an increase in the overall cost of the test piece.

In order to overcome the above disadvantages, the present invention provides a substrate for a fluid detecting test piece, wherein an upper surface of the substrate is recessed downwardly with at least a first-class channel, the flow path including a first fluid region, a second fluid region and a third fluid region sequentially connected The first fluid zone is for fluid injection. The substrate is characterized in that a nitrocellulose layer is formed at the bottom of the second fluid region and the third fluid region, and the nitrocellulose layer comprises a hollow network configuration, and the second in the flow channel The fluid zone is for fluid transfer, the third fluid zone is for fluid reaction, and the average thickness of the nitrocellulose layer of the second fluid zone is no greater than the thickness of the nitrocellulose layer of the third fluid zone.

Therefore, the main object of the present invention is to provide a substrate for a fluid detecting test piece which has a water-absorbing nitrocellulose layer, thereby avoiding residual fluid to be tested in the flow path.

Another object of the present invention is to provide a substrate for a fluid detecting test piece having a water absorbing nitrocellulose layer. Since the water volume per unit volume of nitrocellulose is constant, the volume of the nitrocellulose layer on the substrate can be set. , while providing quantitative detection of fluids.

Another object of the present invention is to provide a substrate for a fluid detecting test piece having a hollow network structure of a nitrocellulose layer, and the bubbles in the fluid are destroyed due to the fluid flowing through the hollow network configuration, so Larger bubbles prevent the occurrence of bubbles blocking the flow path in the microfluidic technology, which in turn affects the quantitative analysis results.

Since the present invention discloses a substrate for a fluid detecting test piece, the physical, chemical, and solution coating techniques utilized therein are well known to those of ordinary skill in the art, and therefore, the description below will not be fully described. At the same time, the drawings in the following texts are indicative of the features related to the features of the present invention, and are not required to be completely drawn according to the actual situation.

FIG. 1A is a schematic view showing a substrate of a fluid detecting test piece according to a first preferred embodiment of the present invention. The substrate 1 of the fluid detecting test piece is recessed downward from the upper surface 10 with a flow path 11 including a first fluid region 111, a second fluid region 112 and a third fluid region 113 which are sequentially connected. The first fluid zone 111 is for the injection of a fluid. After the fluid is injected into the first fluid zone 111, the fluid is transferred to the third fluid zone 113 via the second fluid zone 112, and the component to be tested in the fluid is performed in the third fluid zone 113. reaction. Also, in a preferred embodiment, the substrate 1 is a biocompatible material.

Please refer to Figure 1B for a cross-sectional view along line AA in Figure 1A. The substrate 1 is characterized in that nitrocellulose layers 1121 and 1131 are formed at the bottom of the second fluid region 112 and the third fluid region 113, and the nitrocellulose layers 1121 and 1131 have a hollow mesh shape, which is porous. Hollow network structure, which can absorb the fluid flowing in from the first fluid zone 111, so that the component to be tested in the fluid exists The reagent in the nitrocellulose layer 1131 is reacted. Since the nitrocellulose layers 1121 and 1131 are water absorbing materials, fluid can be prevented from remaining in the flow path 11, and bubbles in the fluid are destroyed as the fluid flows through the nitrocellulose layers 1121 and 1131 of the hollow network configuration. Therefore, it is possible to prevent the bubble from blocking the flow path 11. In addition, the average thickness Da of the nitrocellulose layer 1121 of the second fluid region 112 is not greater than the thickness Db of the nitrocellulose layer 1131 of the third fluid region 113, that is, Da may be less than or equal to Db. Further, in order to reduce the volume of the desired biological specimen, the widths Wa and Wb of the second fluid region 112 and the third fluid region 113 (as shown in Fig. 1A) are preferably at least 0.3 mm.

In the production, the nitrocellulose layers 1121 and 1131 are formed by mixing a nitrocellulose powder with an organic solvent containing esters and ketones to form a nitrocellulose solution; The subsequent nitrocellulose solution is cast in the bottom of the second fluid zone 112 and the third fluid zone 113. After drying, a nitrocellulose layer 1121 is formed at the bottom of the second fluid zone 112, and in the third fluid zone. The bottom of 113 forms a nitrocellulose layer 1131. For better casting, the surface roughness (Ra value) of the flow path 11 is preferably between 3 and 50 microns.

After the nitrocellulose solution is dried, a nitrocellulose layer having a hollow network configuration is formed. In order to adjust a preferred hollow network configuration, the nitrocellulose powder of the nitrocellulose solution of the present invention is mixed with an organic solvent containing an ester and a ketone. The preferred volume ratio is 1:9. Since the water absorption per unit volume of the nitrocellulose is constant, the volume of the corresponding nitrocellulose solution can be derived from the volume of the fluid to be absorbed, and then cast. This makes it possible to fix the volume of the liquid required for detection and to be suitable for micro-testing.

The first preferred embodiment described above is a test strip substrate having three fluid regions, and in accordance with the spirit of the present invention, a fourth fluid region may be added to the flow path for storing excess fluid in the flow channel. A test strip substrate having four fluid regions of the second embodiment of the present invention will be described below.

Please refer to FIG. 2A, which is a schematic diagram of a substrate of a fluid detecting test piece according to a second embodiment of the present invention. The substrate 2 is recessed from the upper surface 20 with a flow path 21, and the flow path 21 includes a first fluid region 211, a second fluid region 212, a third fluid 213 region and a fourth fluid region 214 which are sequentially connected. The first fluid zone 211 is for the injection of fluid, and after the fluid is injected into the first fluid zone 211, the second fluid zone 212 is transferred. Upon reaching the third fluid zone 213, the component to be tested in the fluid reacts in the third fluid zone 213.

Please refer to Figure 2B for a cross-sectional view along line AA of Figure 2A. The nitrocellulose layers 2121 and 2131 are formed at the bottom of the second fluid region 212 and the third fluid region 213, and the average thickness Dc of the nitrocellulose layer 2121 of the second fluid region 212 is equal to the thickness Dd of the third fluid region 213 nitrocellulose layer 2131. . The bottom of the fourth fluid zone 214 is identical to the second and third fluid zones 212, 213, and is also formed with a nitrocellulose layer 2141 and also in a hollow mesh configuration for storage of excess fluid. The nitrocellulose layer 2141 at the bottom of the fourth fluid zone 214 is identical in fabrication to the nitrocellulose layers 2121 and 2131 at the bottom of the second and third fluid zones 212, 213, and is cast in the second fluid zone with the nitrocellulose solution. 212. The third fluid zone 213 and the bottom of the fourth fluid zone 214 are formed by drying.

In addition, in the present embodiment, the structure, the size and the interconnection relationship of the first fluid region, the second fluid region, and the third fluid region, the preferred material of the substrate, the surface roughness, the configuration of the nitrocellulose layer in the substrate, and the formation manner thereof The components and preferred ratios of the nitrocellulose solution used are the same as those of the first embodiment, and thus the description thereof will not be repeated here.

The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention. The above description is to be understood by those skilled in the art, and thus the other embodiments are not disclosed. Equivalent changes or modifications made in the spirit of the invention are to be included in the scope of the claims below.

Substrate ‧‧1,2

Upper surface ‧‧10,20

11, 21‧‧‧ runners

111, 211‧‧‧ first fluid zone

112, 212‧‧‧Second fluid zone

113, 213‧‧‧ third fluid zone

214‧‧‧Fourth fluid zone

1121, 13, 2121, 2131, 2141‧‧ ‧ nitrocellulose layer

Da‧‧‧ average thickness of nitrocellulose layer

Db‧‧‧Nitrocellulose layer thickness

Dc‧‧‧ average thickness of nitrocellulose layer

Dd‧‧‧Nitrocellulose layer thickness

Wa‧‧‧Width of the second fluid zone

Wb‧‧‧ width of the third fluid zone

Fig. 1A is a schematic view showing a substrate of a fluid detecting test piece according to a first embodiment of the present invention.

Fig. 1B is a schematic view showing a cross section of a substrate of a fluid detecting test piece according to a first embodiment of the present invention.

Fig. 2A is a schematic view showing a substrate of a fluid detecting test piece according to a second embodiment of the present invention.

Fig. 2B is a schematic view showing a cross section of a substrate of a fluid detecting test piece according to a second embodiment of the present invention.

Substrate ‧‧1

First fluid area ‧‧11111

Second fluid zone ‧‧‧112

Third fluid zone ‧‧‧113

Nitrocellulose layer ‧‧‧12,13

Average thickness of nitrocellulose layer ‧‧Da

Nitrocellulose layer thickness ‧‧‧Db

Width of the second fluid zone ‧‧Wa

Width of the third fluid zone ‧‧‧Wb

Claims (8)

a substrate for a fluid detecting test piece, the substrate is recessed downwardly from the upper surface thereof, at least a first channel, the flow channel comprising a first fluid zone, a second fluid zone and a third fluid zone sequentially connected, the first fluid zone The injection of the fluid is characterized in that: a nitrocellulose layer is formed at the bottom of the second fluid zone and the third fluid zone, and the nitrocellulose layer comprises a hollow network structure, wherein the nitrocellulose layer is composed of a nitrocellulose solution. Formed, and the nitrocellulose solution is formed by mixing nitrocellulose powder with an ester and a ketone solvent in a preferred ratio of 1:9, the second fluid zone is for fluid transfer, and the third fluid zone is The fluid is reacted, and the average thickness of the nitrocellulose layer of the second fluid zone is not greater than the thickness of the nitrocellulose layer of the third fluid zone, and the minimum width of the second fluid zone and the third fluid zone is 0.3 mm. The substrate of the fluid detecting test piece according to claim 1, wherein the average thickness of the nitrocellulose layer of the second fluid region is smaller than the thickness of the nitrocellulose layer of the third fluid region. The substrate for a fluid detecting test piece according to claim 2, wherein the nitrocellulose layer is formed by pouring a nitrocellulose solution into the bottom of the second fluid zone and the third fluid zone and drying. The substrate of the fluid detecting test piece of claim 2, wherein the flow path has a surface roughness of between 3 micrometers and 50 micrometers. The substrate of the fluid detecting test piece of claim 1, wherein the average thickness of the nitrocellulose layer of the second fluid zone is equal to the thickness of the nitrocellulose layer of the third fluid zone. The substrate for a fluid detecting test piece according to claim 5, wherein the flow path further comprises a fourth fluid region, and a bottom of the fourth fluid region is also formed with a nitrocellulose layer, the nitrocellulose layer comprising a hollow network structure Type for storage of excess fluid. The substrate for a fluid detecting test piece according to claim 6, wherein the nitrocellulose layer is formed by pouring a nitrocellulose solution into the second fluid zone, the third fluid zone and the bottom of the fourth fluid zone, and then drying. . A substrate for a fluid detecting test piece according to claim 6 wherein the surface roughness of the flow path is between 3 micrometers and 50 micrometers.