200942168 六、發明說明： 【發明所屬之技術領域】 本發明係關於藉由以保存液取代切花中之組織液，可 延長該切花壽命之切花的保存方法、及壽命延長之加工切 花的製造方法。 【先前技術】 © 近年來，稱爲保鮮花（Preserved Flower)之加工切 花受到注目。所謂保鮮花係對玫瑰或康乃馨等之切花，以 保存液取代該組織中的水分（以下稱爲組織液）者，因爲 比乾燥花留有更近於鮮花之形態（形狀、質感、花色等） ，所以作爲獨特的花材，使用於婚禮之捧花或一般家庭玄 關·客廳之裝飾等變多。 作爲如此保鮮花之先前的製造方法，已知例如下述專 利文獻1或專利文獻2所揭示之方法。 〇 [專利文獻1]特許第3 548744號公報 [專利文獻2]特開2〇〇7_11 9459號公報 【發明內容】 [發明之揭示] 然而，於前述專利文獻1所記載之方法，於·高溫多 '濕 之條件下，有花的劣化快速，花瓣的色調變白、或部份變 得透明等之問題。另外，於前述專利文獻2所記載之方法 ，依據處理切花的品種，不一定順利地將取代切花中組織 200942168 液之親水性有機溶劑，取代成保存液之步驟，有時不能充 份地維持該切花的形狀（大小、及形狀）。 本發明係有鑑於前述實際情形者，該目的係提供可更 安定地維持切花形狀之切花的保存方法、及加工切花的製 造方法。 有關本發明之切花的保存方法之第1特徵手段係藉由 以保存液取代切花中之組織液，保存該切花之方法，包含 以下步驟：1.將前述切花中之組織液取代成親水性有機溶 劑之第1取代步驟；2.將取代前述切花中之前述組織液之 前述親水性有機溶劑，取代成含有與前述親水性有機溶劑 具有親和性且不揮發性或難揮發性之有機溶劑之保存液、 及前述親水性有機溶劑之混合液之第2取代步驟。 [作用及功效] 如後述實施例2所示，於本發明中，使用含有與親水 性有機溶劑具有親和性且不揮發性或難揮發性之有機溶劑 © 之保存液、及親水性有機溶劑之混合液，與直接使用保存 液之傳統方法比較時，容易維持切花形狀，可更安定地保 存切花近於原本鮮花的形狀。 有關本發明之切花的保存方法之第2特徵手段係藉由 以保存液取代切花中之組織液，保存該切花之方法，包含 以下步驟：1.將前述切花中之組織液取代成親水性有機溶 劑之第1取代步驟；2.將取代前述切花中之前述組織液之 前述親水性有機溶劑，取代成含有與前述親水性有機溶劑 -6 _ 200942168 具有親和性且不揮發性或難揮發性之有機溶劑之保存液、 及前述親水性有機溶劑之第1混合液之第2取代步驟；3. 前述切花中之第1混合液係含有前述親水性有機溶劑及前 述保存液之混合液，以前述保存液之含量比前述第1混合 液多之第2混合液取代之第3取代步驟。 [作用及功效] φ 本發明係包含取代成含有與親水性有機溶劑具有親和 性且不揮發性或難揮發性之有機溶劑之保存液、及親水性 有機溶劑之第1混合液之第2取代步驟，以及前述切花中 之第1混合液係含有前述親水性有機溶劑及前述保存液之 混合液，以前述保存液之含量比前述第1混合液多之第2 混合液取代之第3取代步驟。 亦即’本發明係階段地逐漸地增加使用混合液中之保 存液之含量，可實施複數取代步驟者，如後述實施例3所 © 示，與直接使用保存液之傳統方法比較時，容易維持切花 形狀’並且亦耐乾燥’可更安定地保存切花近於原本鮮花 的形狀。 有關本發明之切花的保存方法之第3特徵手段係前述 親水性有機溶劑係含有至少1種選自乙醇、正丙醇、異丙 醇所成群之液體。 [作用及功效] 含有至少1種選自乙醇、正丙醇、異丙醇所成群之液 -7- 200942168 體之親水性有機溶劑係如後述實施例1所示’浸漬切花於 該親水性有機溶劑中時，切花所含之天然色素不易溶脫’ 且切花亦不易收縮。 另外，前述親水性有機溶劑具有之如此性質係依據本 發明者等之努力硏究，首先被發現者。 有關本發明之切花的保存方法之第4特徵手段係前述 保存液係聚丙二醇。 ❹ [作用及功效] 如後述實施例2及實施例3所記載，含聚丙二醇之混 合液係可長期間保存切花近於原本鮮花的形態（形狀、質 感、花色等）。尤其，因爲該混合液係不易溶脫切花所含 之天然色素（花青素等）’容易保持天然色素原本狀態於 切花中，所以可保持切花的花色近於原本花朵的花色，或 保持相同的花色。 有關本發明之切花的保存方法之第5特徵手段係前述 Θ 混合液中聚丙二醇之含量係1容積％〜8 0容積％。 [作用及功效] 使用100 %之聚丙二醇，有（1)發生小花及花瓣逐漸 萎縮、（2)於取代後之洗淨步驟容易發生洗淨不佳、（3 )因聚丙二醇之吸濕力高’引起形狀崩壞、（4)經常成 沾濕狀態等之必須解決的幾個問題，但以與乙醇 '異丙醇 、正丙醇等之醇類之混合液進行取代，可解決此等問題。 -8 - 200942168 有關本發明之切花的保存方法之第6特徵手段係前述 第1混合液中聚丙二醇之含量係1容積％〜40容積％，前 述第2混合液中聚丙二醇之含量係41容積。/❶〜80容積％。 [作用及功效] 藉由以具有1容積％〜40容積％之聚丙二醇濃度之第 1混合液取代後，再以具有41容積％〜8 0容積％之聚丙二 〇 醇濃度之第2混合液取代，可抑制花瓣的萎縮’維持更安 定的切花形狀。 有關本發明之切花的保存方法之第7特徵手段係更包 含藉由前述親水性有機溶劑或前述親水性有機溶劑以外之 親水性有機溶劑，沖洗前述切花表面之洗淨步驟。 [作用及功效] 經過藉由含親水性有機溶劑及保存液之混合液之取代 © 步驟之切花係因附著於該表面之保存液而有不自然色澤或 質感。 因此，若依據本發明，以親水性有機溶劑沖洗以洗掉 附著於切花表面之保存液，可使該切花的花色或質感更接 近原本鮮花者。 有關本發明之切花保存用套組之特徵組成係可使用於 具備前述特徵手段之切花的保存方法，至少具備前述親水 性有機溶劑及前述保存液。 -9 - 200942168 [作用及功效] 若使用本發明之切花保存用套組，可簡便地實施具備 前述特徵手段之切花的保存方法。 有關本發明之加工切花的製造方法之第1特徵手段係 藉由以保存液取代切花中之組織液，製造該加工切花之方 法，包含以下步驟：1.將前述切花中之組織液取代成親水 性有機溶劑之第1取代步驟；2.將取代前述切花中之前述 組織液之前述親水性有機溶劑，取代成含有與前述親水性 有機溶劑具有親和性且不揮發性或難揮發性之有機溶劑之 保存液、及前述親水性有機溶劑之混合液之第2取代步驟 [作用及功效] 如後述實施例2所示，於本發明中，使用含有與親水 性有機溶劑具有親和性且不揮發性或難揮發性之有機溶劑 之保存液、及親水性有機溶劑之混合液，與直接使用保存 ◎ 液之傳統方法比較時，容易維持切花形狀，可更安定地保 存切花近於原本鮮花的形狀。 有關本發明之加工切花的製造方法之第2特徵手段係 藉由以保存液取代切花中之組織液，製造加工切花之方法 ，包含以下步驟：1.將前述切花中之組織液取代成親水性 有機溶劑之第1取代步驟；2.將取代前述切花中之前述組 織液之前述親水性有機溶劑’取代成含有與前述親水性有 機溶劑具有親和性且不揮發性或難揮發性之有機溶劑之保 -10- 200942168 存液、及前述親水性有機溶劑之第1混合液之第2取代步 驟；3.前述切花中之第1混合液係含有前述親水性有機溶 劑及前述保存液之混合液，以前述保存液之含量係比前述 第1混合液多之第2混合液取代之第3取代步驟。 [作用及功效] 本發明係包含取代成含有與親水性有機溶劑具有親和 φ 性且不揮發性或難揮發性之有機溶劑之保存液、及親水性 有機溶劑之第1混合液之第2取代步驟，以及前述切花中 之第1混合液係含有前述親水性有機溶劑及前述保存液之 混合液，以前述保存液之含量比前述第1混合液多之第2 混合液取代之第3取代步驟。 亦即，本發明係階段地逐漸地增加使用混合液中之保 存液之含量，可實施複數的取代步驟者，如後述實施例3 所示，與直接使用保存液之傳統方法比較時，容易維持切 〇 花形狀，並且亦耐乾燥，可更安定地保存切花近於原本鮮 花的形狀。 . 有關本發明之切花的製造方法之第3特徵手段係前述 親水性有機溶劑含有至少1種選自乙醇、正丙醇、異丙醇 所成群之液體。 [作用及功效] 含有至少1種選自乙醇、正丙醇、異丙醇所成群之液 體之親水性有機溶劑係如後述實施例1所示，浸漬切花於 -11 - 200942168 該親水性有機溶劑中時，切花所含之天然色素不易溶脫， 且切花亦不易收縮。 另外，前述親水性有機溶劑具有之性質係依據本發明 者等之努力硏究，首先被發現者。 有關本發明之切花的製造方法之第4特徵手段係前述 保存液係聚丙二醇。 [作用及功效] 如後述實施例2及實施例3所記載，含聚丙二醇之混 合液係可長期間保存切花近於原本鮮花的形態（形狀、質 感、花色等）。尤其，因爲該混合液係不易溶脫切花所含 之天然色素（花青素等），容易保持天然色素原本狀態於 切花中’所以可保持切花的花色近於原本花朵的花色，或 保持相同花色。 有關本發明之切花的製造方法之第5特徵手段係前述 混合液中聚丙二醇之含量係1容積％〜80容積％。 [作用及功效] 使用100%之聚丙二醇’有（1)發生小花及花瓣逐漸 萎縮、（2)於取代後之洗淨步驟容易發生洗淨不佳、（3 )因聚丙二醇之吸濕力高，引起形狀崩壞、（4)經常成 沾濕狀態等之必須解決的幾個問題，但以與乙醇、異丙醇 、正丙醇等之醇類之混合液進行取代，可解決此等問題。 有關本發明之加工切花的製造方法之第6特徵手段係 -12- 200942168 前述第1混合液中聚丙二醇之含量係1容積％〜40容積％ ，前述第2混合液中聚丙二醇之含量係41容積％〜80容 積％。 [作用及功效] 藉由以具有1容積％〜40容積％之聚丙二醇濃度之第 1混合液取代後，再以具有41容積％〜80容積％之聚丙二 ❹ 醇濃度之第2混合液取代，可抑制花瓣的萎縮，維持更安 定的切花形狀。 有關本發明之切花的製造方法之第7特徵手段係更包 含藉由前述親水性有機溶劑或前述親水性有機溶劑以外之 親水性有機溶劑，沖洗前述切花表面之洗淨步驟。 [作用及功效] 經過藉由含親水性有機溶劑及保存液之混合液之取代 ® 步驟之切花係因附著於該表面之保存液而有不自然色澤或 質感。 • 因此’若依據本發明’以親水性有機溶劑沖洗以洗掉 附著於切花表面之保存液，可使該切花之花色或質感更接 近原本鮮花者。 有關本發明之加工切花之特徵組成係藉由具備前述特 徵手段之加工切花的製造方法所製造。 [作用及功效] -13- 200942168 本發明之加工切花係可長期維持近於原 本鮮花之形狀 [用以實施發明之最佳型態] 於說明本發明之實施型態之前，說明專 說明書所使用之用語如下。 (切花） 作爲可適用本發明之切花，可舉例如 Symphony Marine、King of Blue Picotee Picotee、Diamond Peach ' Ex Lous a white ' 、飛燕草（Delphinium ) ( Aurora Blue inp Blue)等，但並非侷限於此等者。 (組織液） 本發明中所謂的組織液係指切花組織中 主要爲水分）者。 利申請範圍及 ❹ 土耳其桔梗（ 、P icolo Pink Rosina Pink ) 、Triton Light 存在的液體（ Ο (加工切花） 本發明中所謂的加工切花係指對前述切 後述第1取代步驟及第2取代步驟者，另外 再施以洗淨步驟或乾燥步驟之切花的加工品 加工切花係將鮮花具有的水分實質上取代成 花比較’提升保存性。 花，至少施以 ，因應需要， 。本發明中之 保存液，與鮮 -14- 200942168 (親水性有機溶劑） 本發明中所謂的親水性有機溶劑係指至少與水 和性，且具有可取代切花組織液之性質之有機溶劑 ，作爲如此之親水性有機溶劑，進一步不易溶脫切 含的天然色素，並且於後述之第1取代步驟中浸漬 ，具有不易使切花發生收縮之性質者爲宜。詳細而 Φ 配係數logP (分子之親疏水性之參數）爲-0.271 附近之低黏性親水性有機溶劑爲宜。 可適用於本發明之親水性有機溶劑，可舉例如 少1種選自乙醇、正丙醇、異丙醇所成群之液體者 ，作爲本發明中之親水性有機溶劑，可自前述乙醇 醇、異丙醇中選擇任一種使用，或自此等醇類中， 要，可任意選擇複數的醇混合，使用該混合物。另 其切花爲白花時，爲保持更佳的花色，亦可使用於 ❿ 類或此等之混合物，再加入一定量之甲醇者。 . (保存液） 本發明中所謂的保存液係指至少與前述親水性 劑具有親和性，且不揮發性或難揮發性之有機溶劑 ，作爲如此保存液，以進一步具有不易溶脫切花中 天然色素之性質之有機溶劑爲宜。詳細而言，於不 ，分配係數logP (分子之親疏水性之參數）爲-I 2.8266附近，並且於常溫爲液體之保存液係因爲花 .具有親 。另外 花中所 切花時 言，分 〜0.344 含有至 。亦即 、正丙 因應需 外，尤 前述醇 有機溶 。另外 所含的 揮發性 1.236 ~ 瓣形狀 200942168 之保存性優異，所以適宜。 作爲可適用於本發明之保存液，雖可舉例如聚丙二醇 等，但並非侷限於此者。 以下係說明本發明（切花的保存方法及切花的製造方 法）之實施型態。 [第1實施型態] 本實施型態係包含以下之（1)第1取代步驟、及（2 Q )第2取代步驟者，因應需要，亦可實施（3)洗淨步驟 及（4)乾燥步驟。 (1 )第1取代步驟 首先，使準備的切花，於放入前述之親水性有機溶劑 之容器內，浸漬規定時間（例如4〜24小時程度），將切 花之組織中之組織液取代成親水性有機溶劑。 ❹ (2)第2取代步驟 接著，將前述第1取代步驟經脫水處理之切花，於放 入含有前述第1取代步驟使用之親水性有機溶劑、及前述 之保存液之混合液之容器內，浸漬規定時間（例如1 〇〜24 小時程度），將切花之組織中之親水性有機溶劑取代成混 合液。 另外，於本實施型態中，使用聚丙二醇作爲保存液時 ，混合液中之聚丙二醇之含量係以1容積％〜80容積％爲 -16- 200942168 宜。 (3) 洗淨步驟 於前述第2取代步驟之後，使用後述之洗淨液，輕輕 沖洗該切花表面’洗掉附著於切花表面之保存液。另外， 此洗淨步驟係於前述第2取代步驟中尤其使用不揮發性之 有機溶劑作爲保存液時’有效地除去附著於切花表面之該 ® 不揮發性有機溶劑。 (洗淨液） 作爲本發明中可使用之洗淨液係除了水以外之溶劑， 只要可洗掉附著於切花表面之保存液之溶劑，可使用任意 的溶劑。 作爲如此洗淨液’雖可舉例如前述之親水性有機溶劑 ，但不侷限於此等者。另外，作爲洗淨液使用之親水性有 參 機溶劑係不侷限於前述第1取代步驟使用之親水性有機溶 劑，因應需要，亦可使用該親水性有機溶劑以外之其他親 . 水性有機溶劑。 (4) 乾燥步驟 最後，將前述洗淨步驟結束沖洗後的切花乾燥，除去 殘留於該表面之洗淨液。另外，乾燥方法係靜置、風乾、 溫風之送風等之不造成經前述各處理步驟之切花（加工切 花）形態影響之溫度•濕度條件下（例如室溫爲3 5 °C，濕 -17- 200942168 度爲70%以下）之乾燥爲宜。乾燥時間係可任意設定數分 〜數週程度。 依據本實施型態’與直接使用保存 '液之傳統方法比較 ，容易維持切花的形狀’可更安定地保存切花近於原本鮮 花之形狀。 認爲此乃因親水性有機溶劑及保存液之混合液與親水 性有機溶劑之比重差係比保存液與親水性有機溶劑之比重 差小，所以混合液與親水性有機溶劑之取代比保存液與親 水性有機溶劑之取代快速實施。 [第2實施型態] 本實施型態係包含以下之（1)第1取代步驟、（2) 第2取代步驟及（3)第3取代步驟者，因應需要，亦可 實施（3)洗淨步驟及（4)乾燥步驟。 (1 )第1取代步驟 首先’使準備的切花，於放入前述之親水性有機溶劑 之容器內’浸漬規定時間（例如4〜24小時程度），將切 花之組織中之組織液取代成親水性有機溶劑。 (2)第2取代步驟 接著，將前述第1取代步驟經脫水處理之切花，於放 入含有前述第1取代步驟使用之親水性有機溶劑、及前述 之保存液之第1混合液之容器內，浸漬規定時間（例如1〇 200942168 〜24小時程度），將切花之組織中之親水性有機溶劑取代 成第1混合液。 使用聚丙二醇作爲保存液時，第1混合液中之聚丙二 醇之含量係以1容積％〜80容積％爲宜，以1〇容積％〜50 容積％尤佳，以20容積％〜40容積％更好。 (3 )第3取代步驟 〇 接著，將前述第2取代步驟經處理的切花，於放入含 有前述第2取代步驟使用之親水性有機溶劑及保存液，該 保存液含量係比前述第1混合液多之第2混合液之容器內 ，浸漬規定時間（例如1 0〜2 4小時程度），將切花之組 織中之第1混合液取代成第2混合液。 使用聚丙二醇作爲保存液時，第2混合液中之聚丙二 醇之含量係以1容積％〜8 〇容積％爲宜，以4 1容積％〜8 0 容積％尤佳’以6 0容積％〜8 〇容積％更好。 ® 另外’於本實施型態中’因應需要，亦可於前述第2 取代步驟及第3取代步驟之後，逐漸增加混合液中保存液 -之含里’實施進一步的取代步驟（第4取代步驟、第5取 代步驟等.........）（第2取代步驟使用之第1混合液中保 存液之含量 <第3取代步驟使用之第2混合液中保存液之 含量〈第4取代步驟使用之第3混合液中保存液之含量< (4 )洗淨步驟 -19- 200942168 於前述第3取代步驟之後，使用後述之洗淨液，輕輕 沖洗該切花表面，洗掉附著於切花表面之保存液。另外， 此洗淨步驟係於前述第2取代步驟及第3取代步驟中，尤 其使用不揮發性之有機溶劑作爲保存液時，有效地除去附 著於切花表面之該不揮發性有機溶劑。 (洗淨液） 作爲本發明中可使用之洗淨液係除了水以外之溶劑， 只要可洗掉附著於切花表面之保存液之溶劑，可使用任意 的溶劑。 作爲如此洗淨液，雖可舉例如前述之親水性有機溶劑 ’但不侷限於此等者。另外，作爲洗淨液使用之親水性有 機溶劑係不限於前述第1取代步驟使用之親水性有機溶劑 ，因應需要，亦可使用該親水性有機溶劑以外之其他親水 性有機溶劑。 (5 )乾燥步驟 最後，將前述洗淨步驟結束沖洗後的切花乾燥，除去 殘留於該表面之洗淨液。另外，乾燥方法係靜置、風乾、 溫風之送風等，於不造成經前述各處理步驟之切花（加工 切花）形態影響之溫度•濕度條件下（例如室溫爲3 5 °C， 濕度爲70%以下）之乾燥爲宜。乾燥時間係可任意設定數 分〜數週程度。 依據本實施型態，與直接使用保存液之傳統方法比較 -20- 200942168 ，容易維持切花的形狀，且耐乾燥’可更安定地保存切花 近於原本鮮花之形狀。 認爲此乃因親水性有機溶劑及保存液之混合液與親水 性有機溶劑之比重差係比保存液與親水性有機溶劑之比重 差小，所以混合液與親水性有機溶劑之取代比保存液與親 水性有機溶劑之取代快速實施，並且’藉由階段地逐漸地 增加使用混合液中之保存液之含量，實施複數的取代步驟 Q ，與傳統方法比較時，可更順利地實施親水性有機溶劑及 保存液之取代。 [其他實施型態] 1. 本發明並非侷限於前述實施型態者’因應需要，亦 可追加添加人工色素於保存液，將切花再染色之步驟。 2. 本發明可使用至少具備親水性有機溶劑及保存液（ 分別放入於適當容器）之切花保存用套組實施。 e 【實施方式】 .[實施例] 以下係由實施例具體地說明關於本發明，但本發明並 不偈限於此等者。 [比較例] 試驗使用混合乙醇、正丙醇、及異丙醇調製之混合乙 醇（85 _5容積％之乙醇、9.6容積％之正丙醇、4.9容積％ -21 - 200942168 之異丙醇）作爲親水性有機溶劑，實施第1取代步驟，使 用聚丙二醇（100容積％)作爲保存液，實施第2取代步 驟，進一步，以混合乙醇洗淨，自然乾燥之所謂的傳統的 保鮮花製作方法，是否可以重現100L容器之大量生產。 使用土耳其梧梗（Symphony Marine、King of Blue Picotee、Picolo Pink Picotee、Diamond Peach、Ex Lousa white ' Rosina Pink)、飛燕草（Aurora Blue inp、Triton Light Blue) 作爲切花，將此等切花排列於平底深盤，以 100L容器作爲藥劑容器，每1個容器同時處理200〜400 朵。 第1取代步驟係使用前述混合乙醇，進行浸漬6小時 〇 第2取代步驟係使用與前述第1取代步驟同型之容器 ，放入100L之聚丙二醇，將經施以第1取代步驟的切花 移至放入聚丙二醇之容器，浸漬15小時。 第2取代步驟後，以前述混合乙醇數次洗淨（洗淨步 驟）切花，於室內自然乾燥（乾燥步驟）。 (結果及檢討） 自第2取代步驟後明顯發現花瓣內滲出醇，隨著滲出 ，花的尺寸最大縮小40%程度。並且花瓣先端捲曲。 於洗淨步驟中’爲洗掉附著於花基部部份的聚丙二醇 ’需要5分鐘以上的時間。然而’花瓣先端部份係以2〇 秒程度就已過度洗淨’剛乾燥後開始萎縮，不能形成商品 -22- 200942168 可預測此等問題係因藉由使用100容積％之聚丙二醇 而與親水性有機溶劑之比重差（親水性有機溶劑：聚丙二 醇=0.8 : 1.0 04 )變大而引起。因此，認爲減少比重差（ 亦即，混合聚丙二醇及混合乙醇使用）則可避免之可能性 高。 〇 [實施例1 ]檢討可適用於本發明之親水性有機溶劑 使用土耳其梧梗之 Diamond Peach、及飛燕草之 Aurora Blue inp作爲切花，浸漬於下述表1所示之各種親 水性有機溶劑中4小時後（第1取代步驟），與鮮花比較 觀察該脫水狀態、花瓣硬度、莖的硬度、花瓣厚度、顏色 變化。 (結果及檢討） ® 下述表1表示的全部親水性有機溶劑’發現色素保存 〇 . 另外’後述之實施例2及實施例3中’使用第3混合 乙醇（85.5容積％之乙醇、96容積％之正丙醇、4.9容積 %之異丙醇）。 -23- 200942168 [表1] 親水性有機溶劑 脫水狀態 花瓣的硬度 莖的硬度 花瓣厚度 顏色變化 無水乙醇 良好 硬 硬 梢薄 少 正丙醇 良好 硬 硬 稍薄 少 異丙醇 良好 硬 硬 稍薄 少 第1混合乙醇(85.5容積％之 乙醇、13.4容積％之異丙醇） 良好 硬 硬 稍薄 少 第2混合乙醇(88.24容積％之 乙醇、10.1容積％之異丙醇） 良好 硬 硬 稍薄 少 第3混合乙醇(85.5容積％之 乙醇、9.6容積％之正丙醇 、4.9容積％之異丙醇） 良好 硬 稍硬 稍薄 少 [實施例2]前述第1實施型態之實施例 由前述比較例之結果，不僅以聚丙二醇進行第2取代 步驟，根據藉由混用前述實施例1之第3混合乙醇及聚丙 二醇，是否有效地減小比重差之發現，對第3混合乙醇及 聚丙二醇之混合液中聚丙二醇之濃度爲30容積％、50容 積％、70容積％時之狀態，進行比較試驗。 使用 土耳其洁梗之 Picolo Pink Picotee、Mini MoniBACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for preserving a cut flower having a cut flower life by replacing a tissue fluid in a cut flower with a preservation solution, and a method for producing a cut flower having an extended life. [Prior Art] © In recent years, processing cut flowers called Preserved Flowers have attracted attention. The so-called preserved flowers are cut flowers such as roses or carnations, and the water in the tissue (hereinafter referred to as the tissue fluid) is replaced by a preservation solution, because the flowers are more closely related to the shape (shape, texture, color, etc.) of the flowers. Therefore, as a unique flower material, it is used in wedding bouquets or general family porch and living room decoration. For example, the method disclosed in Patent Document 1 or Patent Document 2 below is known as a method of manufacturing the flower. [Patent Document 1] Japanese Laid-Open Patent Publication No. Hei. No. Hei. No. Hei. No. Hei. Under the condition of 'wet', there is a problem that the flower deteriorates rapidly, the color of the petals becomes white, or the part becomes transparent. Further, in the method described in the above Patent Document 2, depending on the type of the cut flower, the step of replacing the hydrophilic organic solvent of the tissue 200942168 in the cut flower with the preservation liquid may not be satisfactorily maintained. Cut the shape (size, and shape) of the flower. The present invention has been made in view of the above circumstances, and it is an object of the present invention to provide a method for preserving cut flowers in a cut shape and a method for producing cut flowers. The first characteristic method of the method for preserving cut flowers according to the present invention is a method for storing the cut flowers by replacing the tissue fluid in the cut flowers with a preservation solution, comprising the steps of: 1. replacing the tissue fluid in the cut flowers into a hydrophilic organic solvent. a first substitution step; 2. replacing the hydrophilic organic solvent that replaces the tissue fluid in the cut flower with a preservation solution containing an organic solvent having affinity with the hydrophilic organic solvent and being non-volatile or difficult to be volatile, and A second substitution step of the mixture of the hydrophilic organic solvents. [Function and Efficacy] As shown in the second embodiment to be described later, in the present invention, a preservation solution containing an organic solvent having affinity with a hydrophilic organic solvent and which is not volatile or difficult to be volatile, and a hydrophilic organic solvent are used. When the mixed solution is compared with the conventional method in which the preservation solution is directly used, it is easy to maintain the shape of the cut flower, and the shape of the cut flower close to the original flower can be more stably preserved. The second characteristic method of the method for preserving cut flowers of the present invention is a method for storing the cut flowers by replacing the tissue fluid in the cut flowers with a preservation solution, comprising the steps of: 1. replacing the tissue fluid in the cut flowers into a hydrophilic organic solvent. a first substitution step; 2. replacing the hydrophilic organic solvent that replaces the tissue fluid in the cut flower with an organic solvent having affinity with the hydrophilic organic solvent-6-200942168 and which is non-volatile or difficult to be volatile a second substitution step of the first liquid mixture of the storage liquid and the hydrophilic organic solvent; 3. The first mixed liquid in the cut flower contains a mixed liquid of the hydrophilic organic solvent and the storage liquid, and the preservation liquid The third substitution step in which the second mixed liquid having a larger content than the first mixed liquid is substituted. [Function and effect] φ The present invention includes a second substitution of a first mixed liquid containing a preservative solution containing an organic solvent having affinity with a hydrophilic organic solvent and being nonvolatile or difficult to be volatile, and a hydrophilic organic solvent. And the third mixed liquid in which the first mixed liquid of the cut flowers contains the mixed liquid of the hydrophilic organic solvent and the storage liquid, and the second mixed liquid having a larger content of the storage liquid than the first mixed liquid is substituted . That is, the present invention gradually increases the content of the preservation solution in the mixed solution, and the plural substitution step can be carried out, as shown in the following Example 3, which is easy to maintain when compared with the conventional method of directly using the preservation solution. Cut the flower shape 'and also dry-resistant' to preserve the shape of the cut flower closer to the original flower. The third characteristic means for the method for preserving the cut flowers of the present invention is that the hydrophilic organic solvent contains at least one liquid selected from the group consisting of ethanol, n-propanol and isopropanol. [Function and Efficacy] A hydrophilic organic solvent containing at least one liquid selected from the group consisting of ethanol, n-propanol, and isopropanol is as described in Example 1 below, which is impregnated and cut into the hydrophilicity. In an organic solvent, the natural pigment contained in the cut flower is not easily dissolved and the cut flower is not easily shrinkable. Further, the above-described properties of the hydrophilic organic solvent are first discovered in accordance with the efforts of the inventors and the like. A fourth characteristic means for the method for preserving cut flowers according to the present invention is the above-mentioned preservation liquid polypropylene glycol. ❹ [Operation and efficacy] As described in Example 2 and Example 3 to be described later, the polypropylene glycol-containing mixed liquid can store the cut flowers in a form close to the original flowers (shape, texture, color, etc.) for a long period of time. In particular, since the mixed liquid is not easily dissolved, the natural pigment (anthocyanin, etc.) contained in the cut flower is easy to maintain the original state of the natural pigment in the cut flower, so that the cut flower can be kept close to the original flower color, or remain the same. Color. The fifth characteristic means for the method for preserving the cut flowers of the present invention is that the content of the polypropylene glycol in the hydrazine mixture is from 1% by volume to 80% by volume. [Function and efficacy] Using 100% polypropylene glycol, (1) gradual shrinkage of small flowers and petals, (2) poor washing after substitution, and (3) hygroscopicity due to polypropylene glycol There are several problems that must be solved in order to cause a shape collapse, (4) often in a wet state, etc., but it can be solved by substituting with a mixture of alcohols such as isopropyl alcohol or n-propanol. problem. -8 - 200942168 The sixth characteristic method of the method for preserving cut flowers according to the present invention is that the content of the polypropylene glycol in the first mixed liquid is 1% by volume to 40% by volume, and the content of the polypropylene glycol in the second mixed liquid is 41% by volume. . /❶~80% by volume. [Function and effect] After the first mixed liquid having a concentration of polypropylene glycol having a volume ratio of 1% by volume to 40% by volume, the second mixed liquid having a concentration of polyacrylonitrile having a volume ratio of 41% by volume to 80% by volume is used. Instead, it can inhibit the shrinkage of the petals' to maintain a more stable cut flower shape. The seventh aspect of the method for preserving cut flowers of the present invention further comprises a step of washing the surface of the cut flower by a hydrophilic organic solvent other than the hydrophilic organic solvent or the hydrophilic organic solvent. [Function and effect] After being replaced by a mixture containing a hydrophilic organic solvent and a preservative solution, the cut flower of the step has an unnatural color or texture due to the preservation solution attached to the surface. Therefore, according to the present invention, by washing with a hydrophilic organic solvent to wash away the preservation solution attached to the surface of the cut flower, the flower color or texture of the cut flower can be brought closer to the original flower. The feature of the cut flower storage kit according to the present invention is that the method for preserving cut flowers having the above-described characteristics can be provided with at least the hydrophilic organic solvent and the preservative solution. -9 - 200942168 [Function and effect] When the cut flower storage kit of the present invention is used, the method of storing cut flowers having the above-described characteristics can be easily carried out. The first feature of the method for producing a cut flower according to the present invention is a method for producing the cut flower by replacing the tissue fluid in the cut flower with a preservation solution, comprising the steps of: 1. replacing the tissue fluid in the cut flower into a hydrophilic organic a first substitution step of the solvent; 2. replacing the hydrophilic organic solvent that replaces the tissue fluid in the cut flower with a preservation solution containing an organic solvent having affinity with the hydrophilic organic solvent and being non-volatile or difficult to be volatile And a second substitution step of the mixed liquid of the hydrophilic organic solvent [Operation and efficacy] As shown in the following Example 2, in the present invention, the use contains affinity with a hydrophilic organic solvent and is non-volatile or difficult to volatilize. When the organic solvent-preserving solution and the hydrophilic organic solvent are compared with the conventional method of directly storing the ◎ liquid, it is easy to maintain the shape of the cut flower, and the cut flower can be more stably preserved in shape close to the original flower. A second aspect of the method for producing a cut flower according to the present invention is a method for producing a cut flower by replacing a tissue fluid in a cut flower with a preservation solution, comprising the steps of: 1. replacing the tissue fluid in the cut flower into a hydrophilic organic solvent; a first substitution step; 2. replacing the hydrophilic organic solvent that replaces the aforementioned tissue fluid in the cut flower into an organic solvent containing an affinity for the hydrophilic organic solvent and which is non-volatile or difficult to be volatile - 200942168 a second substitution step of the storage liquid and the first mixed liquid of the hydrophilic organic solvent; 3. The first mixed liquid in the cut flowers contains a mixed liquid of the hydrophilic organic solvent and the storage liquid, and is preserved as described above The liquid content is a third substitution step in which the second mixed liquid is replaced by more than the first mixed liquid. [Function and Efficacy] The present invention includes a second substitution of a first mixed liquid containing a preservative solution containing an organic solvent having affinity with a hydrophilic organic solvent and being nonvolatile or difficult to be volatile, and a hydrophilic organic solvent. And the third mixed liquid in which the first mixed liquid of the cut flowers contains the mixed liquid of the hydrophilic organic solvent and the storage liquid, and the second mixed liquid having a larger content of the storage liquid than the first mixed liquid is substituted . That is, the present invention gradually increases the content of the preservation solution in the mixed solution, and can perform a plurality of substitution steps, as shown in the following Example 3, which is easy to maintain when compared with the conventional method of directly using the preservation solution. The shape of the cut flower is also resistant to dryness, and the cut flower can be more calmly preserved in the shape of the original flower. In a third aspect of the method for producing a cut flower according to the present invention, the hydrophilic organic solvent contains at least one liquid selected from the group consisting of ethanol, n-propanol, and isopropyl alcohol. [Function and Efficacy] A hydrophilic organic solvent containing at least one liquid selected from the group consisting of ethanol, n-propanol, and isopropanol is as shown in Example 1 below, and is impregnated with cut flowers at -11 - 200942168. In the solvent, the natural pigment contained in the cut flower is not easily dissolved, and the cut flower is not easily contracted. Further, the properties of the hydrophilic organic solvent described above were first discovered in accordance with the efforts of the present inventors. A fourth characteristic means for the method for producing cut flowers according to the present invention is the above-mentioned preservation liquid polypropylene glycol. [Function and effect] As described in the second and third embodiments, the mixed liquid containing polypropylene glycol can retain the form (shape, texture, color, etc.) of the cut flower in the vicinity of the original flower for a long period of time. In particular, because the mixture is not easy to dissolve the natural pigment (anthocyanin, etc.) contained in the cut flower, it is easy to keep the natural pigment in the original state of the cut flower, so the flower color of the cut flower can be kept close to the original flower color, or the same flower color can be maintained. . According to a fifth aspect of the method for producing a cut flower of the present invention, the content of the polypropylene glycol in the mixed solution is from 1% by volume to 80% by volume. [Function and efficacy] Using 100% polypropylene glycol 'has (1) gradual shrinkage of small flowers and petals, (2) poor washing after substitution, and (3) hygroscopicity due to polypropylene glycol There are several problems that must be solved, such as high, causing shape collapse, (4) frequent wettability, etc., but this can be solved by substituting with a mixture of alcohols such as ethanol, isopropanol, and n-propanol. problem. The sixth characteristic method of the method for producing a cut flower according to the present invention is -12-200942168. The content of the polypropylene glycol in the first mixed liquid is 1% by volume to 40% by volume, and the content of the polypropylene glycol in the second mixed liquid is 41. Volume % ~ 80% by volume. [Function and effect] After being substituted with the first mixed liquid having a polypropylene glycol concentration of 1% by volume to 40% by volume, the second mixed liquid having a concentration of polyacrylonitrile having a volume of 41% by volume to 80% by volume is replaced. It can inhibit the shrinkage of the petals and maintain a more stable cut flower shape. The seventh aspect of the method for producing a cut flower of the present invention further comprises a step of washing the surface of the cut flower by a hydrophilic organic solvent other than the hydrophilic organic solvent or the hydrophilic organic solvent. [Function and effect] The cut flower line which is replaced by a mixture of a hydrophilic organic solvent and a preservative solution has an unnatural color or texture due to the preservation liquid adhered to the surface. • Therefore, if it is washed with a hydrophilic organic solvent to wash away the preservation solution attached to the surface of the cut flower according to the present invention, the flower color or texture of the cut flower can be brought closer to the original flower. The characteristic composition of the cut flower of the present invention is produced by a method for producing a cut flower having the above-described characteristic means. [Function and efficacy] -13- 200942168 The cut flower system of the present invention can maintain the shape of the original flower for a long time [the best mode for carrying out the invention] Before explaining the embodiment of the present invention, the description will be used. The terms are as follows. (cut flower) As the cut flower to which the present invention is applicable, for example, Symphony Marine, King of Blue Picotee Picotee, Diamond Peach 'Ex Lous a white ', Delphinium (Aurora Blue inp Blue), etc., but not limited thereto And so on. (Community fluid) The term "tissue fluid" as used in the present invention means a substance mainly composed of water in a cut flower tissue. Scope of application and liquids of turkey platycodon (Picolo Pink Rosina Pink) and Triton Light ( Ο (process cut flower) The so-called process cut flower in the present invention refers to the first substitution step and the second substitution step described later. In addition, the processed product of the cut flower or the drying step is processed to cut the flower to substantially replace the water with the flowers to improve the preservation. The flower, at least, if necessary, the preservation in the present invention. Liquid, and fresh-14-200942168 (hydrophilic organic solvent) The term "hydrophilic organic solvent" as used in the present invention means an organic solvent having at least water and properties and having a property of replacing the cut flower tissue liquid, as such a hydrophilic organic solvent. Further, it is preferable that the natural pigment which is not easily dissolved and removed is immersed in the first substitution step to be described later, and it is preferable that the cut flower is not easily shrunk. The detailed Φ distribution coefficient logP (parameter of the hydrophilicity of the molecule) is - A low-viscosity hydrophilic organic solvent in the vicinity of 0.271 is suitable. It can be suitably used in the hydrophilic organic solvent of the present invention, for example, less than 1 A liquid selected from the group consisting of ethanol, n-propanol, and isopropyl alcohol, as the hydrophilic organic solvent in the present invention, may be selected from any of the above ethanol alcohols and isopropyl alcohols, or from such alcohols. If necessary, the mixture can be arbitrarily selected and used, and when the cut flower is white flower, in order to maintain a better color, it can also be used in a mixture of hydrazine or the like, and then a certain amount of methanol is added. (Preservation solution) The term "preservation solution" as used in the present invention means an organic solvent having at least affinity with the hydrophilic agent and which is non-volatile or difficult to be volatile, and as such a preservation solution, further has a natural pigment which is not easily dissolved in the cut flower. The organic solvent of the nature is preferred. In detail, the partition coefficient logP (parameter of the hydrophilicity of the molecule) is -I 2.8266, and the liquid preservation liquid at normal temperature is because the flower has a pro. When the flower is cut, it is divided into ~0.344. That is, the positive propane is especially needed, especially the alcohol is organically soluble. The volatility of 1.236 ~ the shape of the valve is 200942168. For the preservation solution to be used in the present invention, for example, polypropylene glycol or the like may be mentioned, but the present invention is not limited thereto. Hereinafter, an embodiment of the present invention (the method for preserving cut flowers and the method for producing cut flowers) will be described. [First embodiment] The present embodiment includes the following (1) first substitution step and (2Q) second substitution step, and if necessary, (3) washing step and (4) Step 1 (1) First substitution step First, the prepared cut flower is placed in a container of the above-mentioned hydrophilic organic solvent, and immersed for a predetermined time (for example, 4 to 24 hours) to replace the tissue fluid in the cut flower tissue. It is a hydrophilic organic solvent. (2) Second Substituting Step Next, the cut flower subjected to the dehydration treatment in the first substitution step is placed in a container containing the hydrophilic organic solvent used in the first substitution step and the mixed solution of the storage solution described above. The impregnation is carried out for a predetermined period of time (for example, about 1 to 24 hours), and the hydrophilic organic solvent in the tissue of the cut flower is replaced with a mixed solution. Further, in the present embodiment, when polypropylene glycol is used as the preservative solution, the content of the polypropylene glycol in the mixed solution is preferably from 1% by volume to 80% by volume, preferably from -16 to 200942168. (3) Washing step After the second substitution step, the surface of the cut flower is gently washed with a washing liquid to be described later, and the preservation liquid adhering to the surface of the cut flower is washed away. Further, this washing step is effective in removing the ® nonvolatile organic solvent attached to the cut surface when the second substitution step is carried out, particularly when a nonvolatile organic solvent is used as the preservative solution. (Cleaning liquid) The washing liquid which can be used in the present invention is a solvent other than water, and any solvent can be used as long as it can wash away the solvent adhering to the preservation liquid on the surface of the cut flower. As such a cleaning liquid, for example, the above-described hydrophilic organic solvent is exemplified, but it is not limited thereto. Further, the hydrophilicity of the solvent used as the cleaning liquid is not limited to the hydrophilic organic solvent used in the first substitution step, and other hydrophilic organic solvents other than the hydrophilic organic solvent may be used if necessary. (4) Drying step Finally, the cut flowers after the washing step is finished are dried to remove the washing liquid remaining on the surface. In addition, the drying method is a temperature/humidity condition such as standing, air drying, warm air blowing, etc., which does not cause the shape of the cut flowers (processed cut flowers) subjected to the foregoing various processing steps (for example, room temperature is 35 ° C, wet -17) - 200942168 degrees below 70%) drying is appropriate. The drying time can be arbitrarily set for a few minutes to several weeks. According to this embodiment, the shape of the cut flower is easily maintained as compared with the conventional method of directly storing the liquid, and the cut flower can be more stably preserved in shape close to the original fresh flower. It is considered that the difference in specific gravity between the mixture of the hydrophilic organic solvent and the preservation solution and the hydrophilic organic solvent is smaller than the difference in specific gravity between the preservation solution and the hydrophilic organic solvent, so the substitution ratio of the mixed solution and the hydrophilic organic solvent is preserved. Substitution with a hydrophilic organic solvent is rapidly carried out. [Second embodiment] The present embodiment includes the following (1) first substitution step, (2) second substitution step, and (3) third substitution step, and may be carried out if necessary (3) a net step and (4) a drying step. (1) The first substitution step first, 'the prepared cut flower is immersed in a container in which the hydrophilic organic solvent is placed in the container for a predetermined period of time (for example, 4 to 24 hours), and the tissue fluid in the cut flower tissue is replaced with hydrophilicity. Organic solvents. (2) Second Substituting Step Next, the cut flower subjected to the dehydration treatment in the first substituting step is placed in a container containing the hydrophilic organic solvent used in the first substituting step and the first mixed liquid of the above-mentioned preservative solution The immersion is performed for a predetermined period of time (for example, 1〇200942168 to 24 hours), and the hydrophilic organic solvent in the cut flower tissue is replaced with the first mixed liquid. When polypropylene glycol is used as the preservative solution, the content of the polypropylene glycol in the first mixed liquid is preferably from 1% by volume to 80% by volume, more preferably from 1% by volume to 50% by volume, and particularly preferably from 20% by volume to 40% by volume. better. (3) Third Substituting Step Next, the cut flower subjected to the second substituting step is placed in a hydrophilic organic solvent and a preservative solution containing the second substituting step, and the content of the preservative solution is mixed with the first The container of the second mixed liquid having a large amount of liquid is immersed for a predetermined time (for example, about 10 to 24 hours), and the first mixed liquid in the structure of the cut flower is replaced with the second mixed liquid. When polypropylene glycol is used as the preservative solution, the content of the polypropylene glycol in the second mixed liquid is preferably 1% by volume to 8% by volume, and is preferably 4% by volume to 80% by volume, and particularly preferably 60% by volume. 8 〇% of volume is better. In addition, in the present embodiment, a further substitution step (fourth substitution step) may be carried out by gradually increasing the concentration of the preservation solution in the mixed solution after the second substitution step and the third substitution step, as needed. The fifth substitution step, etc. (...) (the content of the preservation solution in the first mixed solution used in the second substitution step < the content of the preservation solution in the second mixed solution used in the third substitution step < Content of the preservation solution in the third mixed liquid used in the fourth substitution step < (4) Washing step -19-200942168 After the third substitution step, the surface of the cut flower is gently washed using a washing liquid described later, and washed. And removing the preservation liquid adhering to the surface of the cut flower. The washing step is performed in the second substitution step and the third substitution step, and in particular, when the non-volatile organic solvent is used as the preservation solution, the adhesion to the surface of the cut flower is effectively removed. The non-volatile organic solvent (cleaning liquid) The washing liquid which can be used in the present invention is a solvent other than water, and any solvent can be used as long as the solvent adhering to the preservation liquid on the surface of the cut flower can be washed off. in this way The liquid electrolyte may be, for example, a hydrophilic organic solvent, but is not limited thereto. The hydrophilic organic solvent used as the cleaning liquid is not limited to the hydrophilic organic solvent used in the first substitution step, and the reaction is not limited thereto. It is also possible to use a hydrophilic organic solvent other than the hydrophilic organic solvent. (5) At the end of the drying step, the cut flowers after the washing step is dried to remove the washing liquid remaining on the surface. The method is static, air-drying, warm air supply, etc., under the condition of temperature and humidity which does not affect the morphology of cut flowers (processed cut flowers) of the above various treatment steps (for example, room temperature is 35 ° C, humidity is 70% or less) Drying is suitable. The drying time can be arbitrarily set to several minutes to several weeks. According to this embodiment, compared with the traditional method of directly using the preservation solution -20- 200942168, it is easy to maintain the shape of the cut flower, and is resistant to drying. It is more stable to preserve the shape of the cut flower near the original flower. It is considered that this is due to the proportion of the mixture of hydrophilic organic solvent and preservation solution and hydrophilic organic solvent. The difference in specific gravity between the preservation solution and the hydrophilic organic solvent is small, so the substitution of the mixed solution and the hydrophilic organic solvent is faster than the substitution of the preservation solution and the hydrophilic organic solvent, and 'by gradually increasing the use of the mixed solution The content of the preservation solution is subjected to a plurality of substitution steps Q, and the replacement of the hydrophilic organic solvent and the preservation solution can be performed more smoothly when compared with the conventional method. [Other Embodiments] 1. The present invention is not limited to the foregoing embodiment. In the case of the condition, a step of adding an artificial dye to the preservation solution and dyeing the cut flower may be added. 2. The present invention can be used for the preservation of cut flowers having at least a hydrophilic organic solvent and a preservation solution (individually placed in appropriate containers). [Embodiment] [Embodiment] Hereinafter, the present invention will be specifically described by way of examples, but the present invention is not limited thereto. [Comparative Example] The test was carried out using mixed ethanol (85 _5% by volume of ethanol, 9.6 vol% of n-propanol, 4.9 vol% -21 - 200942168 of isopropanol) prepared by mixing ethanol, n-propanol, and isopropanol as The hydrophilic organic solvent is a so-called conventional method for preparing a preserved flower by performing a first substitution step, using a polypropylene glycol (100% by volume) as a preservation solution, performing a second substitution step, and further washing it with mixed ethanol and drying it naturally. Mass production of 100L containers can be reproduced. Use the Turkish stalks (Symphony Marine, King of Blue Picotee, Picolo Pink Picotee, Diamond Peach, Ex Lousa white 'Rosina Pink), Aurora Blue inp, Triton Light Blue as cut flowers, arrange these cut flowers in flat bottom The tray is a 100 L container as a drug container, and each container is processed at 200 to 400 at the same time. The first substitution step is carried out by using the mixed ethanol for 6 hours. The second substitution step is carried out by using a container of the same type as the first substitution step, and 100 L of polypropylene glycol is placed, and the cut flower subjected to the first substitution step is moved to The container was placed in a polypropylene glycol and immersed for 15 hours. After the second substitution step, the mixed ethanol is washed several times (washing step), and the flowers are naturally dried in the room (drying step). (Results and review) Since the second substitution step, it was found that the petals ooze out of the alcohol. With the exudation, the size of the flower was reduced by a maximum of 40%. And the petals are curled at the apex. It takes more than 5 minutes to wash off the polypropylene glycol attached to the base portion of the flower in the washing step. However, 'the tip end of the petal has been over-washed at a level of 2 sec.' After a drier, it begins to shrink and cannot form a commodity. -22- 200942168 It is predicted that these problems are caused by the use of 100% by volume of polypropylene glycol and hydrophilic. The difference in the specific gravity of the organic solvent (hydrophilic organic solvent: polypropylene glycol = 0.8 : 1.0 04 ) is caused by the increase. Therefore, it is considered that the possibility of reducing the difference in specific gravity (i.e., the use of mixed polypropylene glycol and mixed ethanol) is high. 〇 [Example 1] Review The hydrophilic organic solvent applicable to the present invention is used as a cut flower by Diamond Peach of Turkey stalk and Aurora Blue inp of Delphinium, and immersed in various hydrophilic organic solvents shown in Table 1 below. After 4 hours (the first substitution step), the dehydrated state, the petal hardness, the stem hardness, the petal thickness, and the color change were observed in comparison with the flowers. (Results and Review) ® All of the hydrophilic organic solvents shown in Table 1 below were found to have a pigment storage. In addition, in the second and third embodiments described later, 'the third mixed ethanol (85.5% by volume of ethanol, 96 volumes) was used. % n-propanol, 4.9 vol% isopropanol). -23- 200942168 [Table 1] Dehydration of hydrophilic organic solvent. Hardness of the petal. Hardness of the stem. Thickness of the petal. Color change. Absolute ethanol. Good hard and hard tips. Low n-propanol. Good hard and hard, slightly thin, less isopropyl alcohol, good hard, slightly thinner, less. Mixed ethanol (85.5 vol% ethanol, 13.4 vol% isopropanol) Good hard and slightly thinner second mixed ethanol (88.24 vol% ethanol, 10.1 vol% isopropanol) Good hard and hard slightly thinner third Ethanol (85.5 vol% ethanol, 9.6 vol% n-propanol, 4.9 vol% isopropanol) is hard, slightly hard, slightly thinner and less [Example 2] The first embodiment of the first embodiment is the comparative example described above. As a result, not only the second substitution step was carried out with polypropylene glycol, but also the mixing of the third mixed ethanol and polypropylene glycol was carried out according to whether or not the third mixed ethanol and polypropylene glycol of the above-mentioned Example 1 were mixed to effectively reduce the difference in specific gravity. A comparative test was carried out in a state where the concentration of the polypropylene glycol in the liquid was 30% by volume, 50% by volume, and 70% by volume. Picolo Pink Picotee, Mini Moni
Blue Picotee、Rosina Blue作爲切花，浸漬於第3混合乙 醇中4小時，實施第1取代步驟，接著，浸漬於下述表2 所示之具有各種聚丙二醇濃度之混合液1〜3中15小時， 實施第2取代步驟後，洗淨、乾燥。 另外’作爲對照組係準備僅以聚丙二醇實施第2取代 步驟者。結果如下述表2表示。另外，表2中之記號（+ 及一）係指切花之形狀異常（矮小化、捲曲（反翹）、乾 燥後萎縮）之程度大小，+愈多，該程度大（亦即，（一 -24- 200942168 )< (+-）< ( + ) < ( + + ) < ( + + + ))。 (結果及檢討） 於混合液1(聚丙二醇濃度·· 30容積％)中，未發生 花瓣捲曲及花整體之矮小化。然而，乾燥後花整體乾枯。 於混合液2 (聚丙二醇濃度·· 5 0容積％ )中，雖見到 花瓣捲曲，但未發現花尺寸之矮小化。然而，乾燥後與混 φ 合液1同樣地整體乾枯。 於混合液3(聚丙二醇濃度：70容積％)中，與對照 組大致同樣地發生花瓣捲曲及花整體之矮小化，雖與對照 組同樣地完成’但對於乾燥後花的萎縮，比對照組改善一 〇 關於使用混合液1〜3，實施第2取代步驟之切花，雖 然程度上有差異，但發現比對照組（直接使用保存液之傳 統方法），容易維持切花形狀之改善效果。 ❹ 另外，尤其維持切花形狀係以混合液1(聚丙二醇濃 度：30容積％)佳，爲使乾燥後之保存狀態良好，可知聚 . 丙二醇濃度不可多於50容積％。 [表2] 混合液 聚丙二醇濃度（容積％) 矮小化 捲曲 乾燥後萎縮 1 30 • + + + 2 50 + + 3 70 + + + + + · 對照組 100 + + + + + [實施例3]前述第2實施型態之實施例 -25- 200942168 由前述實施例2之結果，顯示親水性有機溶劑與混合 液之比重差於0.08以內雖不能保持大的擴散•取代能力 ，但若比重差爲0.1以上時，容易引起自花瓣內至花瓣外 之擴散不均。 因此，認爲比重差於0·08以內，混合液之聚丙二醇 濃度自3〇容積％階段地移向濃溶液，形狀不會崩壞而進行 充份的取代。 使用各2個品種之土耳其桔梗之R0Sina BiUe、pieQlD Pink Picotee、Mini Moni Blue Picotee 作爲切花，設定下 述表示之試驗區1〜4。 (試驗區1 ) 試驗區1係依下述步驟處理前述切花。 1·第1取代步驟：浸漬前述切花於第3混合乙醇中4 小時。Blue Picotee and Rosina Blue were cut into the third mixed ethanol for 4 hours, subjected to a first substitution step, and then immersed in the mixed liquids 1 to 3 having various polypropylene glycol concentrations shown in Table 2 below for 15 hours. After the second substitution step, the cells are washed and dried. Further, as the control group, the second substitution step was carried out only with polypropylene glycol. The results are shown in Table 2 below. In addition, the symbols (+ and one) in Table 2 refer to the extent of the shape of the cut flower (dwarf, curl (anti-warping), shrinkage after drying), the more +, the degree is large (that is, (a - 24-200942168 )<(+-)< ( + ) < ( + + ) < ( + + + )). (Results and review) In the mixed solution 1 (polypropylene glycol concentration·30% by volume), the curling of the petals and the overall shortening of the flower did not occur. However, after drying, the flowers are dry as a whole. In the mixed solution 2 (polypropylene glycol concentration··50% by volume), although the petals were curled, the flower size was not found to be short. However, after drying, the whole was dried in the same manner as in the mixed φ liquid mixture 1. In the mixture 3 (polypropylene glycol concentration: 70% by volume), the petal curl and the overall flowering were reduced in the same manner as in the control group, and the same as the control group was completed, but the atrophy of the flower after drying was higher than that of the control group. Improvement of the cut flowers in which the second substitution step was carried out using the mixed liquids 1 to 3, although the degree was different, it was found that it is easier to maintain the shape of the cut flower than the control group (the conventional method of directly using the preservation solution). Further, in particular, it is preferable to maintain the cut flower shape in the mixed liquid 1 (polypropylene glycol concentration: 30% by volume), and it is understood that the concentration of the propylene glycol is not more than 50% by volume in order to maintain a good storage state after drying. [Table 2] Concentration of polypropylene glycol in the mixed solution (% by volume) Dwarfing, shrinking and drying after shrinking 1 30 • + + + 2 50 + + 3 70 + + + + + · Control group 100 + + + + + [Example 3] Example-25-200942168 of the second embodiment described above, as a result of the foregoing Example 2, showing that the difference in specific gravity between the hydrophilic organic solvent and the mixed solution is less than 0.08, although the large diffusion/substitution ability cannot be maintained, but the specific gravity difference is When it is 0.1 or more, it is easy to cause uneven spread from the inside of the petals to the outside of the petals. Therefore, it is considered that the specific gravity is less than 0.08, and the concentration of the polypropylene glycol in the mixed solution is gradually shifted from the volume of 3 〇 to the concentrated solution, and the shape is not collapsed and is sufficiently replaced. R0Sina BiUe, PieQlD Pink Picotee, and Mini Moni Blue Picotee of each of the two varieties of Turkish Campanulaceae were used as cut flowers, and the test areas 1 to 4 shown below were set. (Test Zone 1) Test Zone 1 The above cut flowers were treated in the following procedure. 1. First substitution step: the cut flowers were immersed in the third mixed ethanol for 4 hours.
2·第2取代步驟：將經實施前述第1取代步驟之切 花’浸漬於第1混合液（70容積％之第3混a 7 ^ Λ — 此〇乙醇，3 0容 積％之聚丙二醇）中3小時。 3.第3取代步驟：將經實施前述第2取代步驟之切 花’浸漬於第2混合液（5〇容積％之第3涫 低㈡乙醇，5 0容 積％之聚丙二醇）中2 1小時。 4.洗淨步驟：將經實施前述第3取佧也〜 π步驟之切花， 使用第3混合乙醇洗淨1〇秒。 5_乾燥步驟：將經實施前述洗淨步驟 <切花，於維 -26- 200942168 持室溫爲3 5°C ’濕度爲7〇%以下之乾燥室中，以攪拌扇強 制循環空氣使其乾燥。 (試驗區2 ) 試驗區2係依下述步驟處理前述切花。 1.第1取代步驟··浸漬前述切花於第3海合乙醇中4 小時。 φ 2·第2取代步驟：將經實施前述第1取代步驟之切 花，浸漬於第1混合液（70容積％之第3混合乙醇，30容 積％之聚丙二醇）中3小時。 3.第3取代步驟：將經實施前述第2取代步驟之切 花，浸漬於第2混合液（50容積％之第3混合乙醇，容 積％之聚丙二醇）中4小時。 4·第4取代步驟：將經實施前述第3取代步驟之切 花，浸漬於第3混合液（30容積％之第3混合乙醇，7〇容 φ 積％之聚丙二醇）中1 7小時。 5.洗淨步驟：將經實施前述第4取代步驟之切花， 使用第3混合乙醇洗淨3 0秒（10秒洗淨3次）。 6_乾燥步驟：將經實施前述洗淨歩驟之切&，於 持室溫爲3S°C，濕度爲70%以下之乾燥室中，以擾泮扇強 制循環空氣使其乾燥。 (試驗區3 ) 試驗區3係依下述步驟處理前述切花。 -27- 200942168 1.第1取代步驟：浸漬前述切花於第 小時。 2·第2取代步驟：將經實施前述第1 花，浸漬於第1混合液（70容積％之第3混 積％之聚丙二醇）中3小時。 3. 第3取代步驟：將經實施前述第2 花’浸漬於第2混合液（5〇容積％之第3混 積％之聚丙二醇）中4小時。 4. 第4取代步驟：將經實施前述第3 花，浸漬於第3混合液（40容積％之第3混 積％之聚丙二醇）中1 7小時。 5. 洗淨步騾：將經實施前述第4取^ 使用第3混合乙醇洗淨20秒（10秒洗淨2 6. 乾燥步驟··將經實施前述洗淨步驟 持室溫爲3 5 °C，濕度爲7 0 %以下之乾燥室！：| 制循環空氣使其乾燥。 (試驗區4) 試驗區4係依下述步驟處理前述切花。 1. 第1取代步驟：浸漬前述切花於第 小時。 2. 第2取代步驟：將經實施前述第^ 化，浸漬於第1混合液（7 〇容積％之第3混 積％之聚丙二醇）中3小時。 3混合乙醇中4 取代步驟之切 合乙醇，30容 取代步驟之切 合乙醇，50容 取代步驟之切 合乙醇，60容 :步驟之切花， 次）。 之切花，於維 3 ’以攪拌扇強 3混合乙醇中4 取代步驟之切 合乙醇，30容 200942168 3. 第3取代步驟：將經實施前述第2取代步驟之切 花’浸漬於第2混合液（40容積％之桌3混合乙醇，60容 積％之聚丙二醇）中2 1小時。 4. 洗淨步驟：將經實施前述第3取代步驟之切花， 使用第3混合乙醇洗淨20秒（10秒洗淨2次）。 5. 乾燥步驟：將經實施前述洗淨步驟之切花，於維 持室溫爲35°C，濕度爲70%以下之乾燥室中，以攪拌扇強 . 制循環空氣使其乾燥。 (對照區） 對照區係依下述步驟處理前述切花。 1. 第1取代步驟：浸漬前述切花於第3混合乙醇中4 小時。 2. 第2取代步驟：將經實施前述第1取代步驟之切 花，浸漬於1 0 0容積％之聚丙二醇中1 5小時。 © 3.洗淨步驟：雖將經實施前述第2取代步驟之切花 ，使用第3混合乙醇洗淨，但不能順利進行。 . 4.乾燥步驟：將經實施前述洗淨步驟之切花，於維 持室溫爲35°C，濕度爲7〇%以下之乾燥室中，以攪拌扇強 制循環空氣使其乾燥》 結果如下述表3表示。另外，表3中之記號（+及— )係指切花之形狀異常（矮小化、捲曲（反翹）、乾燥後 萎縮）之程度大小，+愈多，該程度大（亦即，（一）< (+ — )<( + )<( + + )<(+ + + ))。 -29- 200942168 (結果及檢討） 試驗區1〜4之全部試驗區中，切花矮小化、及花瓣 捲曲皆比對照區少。僅試驗區3比其他試驗區稍微矮小化 。洗淨係以試驗區1最簡單，試驗區2係花費試驗區1的 3倍洗淨時間。 試驗區3及試驗區4係比試驗區1需要多1次洗淨時 間。 ❹ 對照區並不能順利洗淨。 藉由以上可得到任一個試驗區1〜4皆比對照區（直 接使用保存液之傳統方法）容易維持切花形狀，另外，對 於乾燥，亦得到具有與對照組相同以上之強度之結果。 [表3] 試驗區 矮小化 捲曲 洗淨時間（秒） 乾燥後萎縮 1 - _ 10 + + 2 1〇χ3 _ 3 + - 10x2 4 - 10x2 + - 對照區 + + + + + + 不良 + + [產業上利用性] 如前述說明，本發明係有效地使用於保存切花、及製 造加工切花，適合長期維持安定的切花形狀。 -30-2. Second substitution step: immersing the cut flower in which the first substitution step is carried out in the first mixed liquid (70% by volume of the third mixed a 7 ^ Λ - this 〇 ethanol, 30% by volume of polypropylene glycol) 3 hours. 3. The third substitution step: the cut flower which was subjected to the second substitution step was immersed in the second mixed liquid (3 涫 5% by volume of the third 涫 low (di)ethanol, and 50% by volume of the polypropylene glycol) for 21 hours. 4. Washing step: The cut flower subjected to the above-mentioned third picking step - π step was washed with the third mixed ethanol for 1 second. 5_ drying step: the above-mentioned washing step <cutting flower is carried out, and in a drying chamber having a room temperature of 35 ° C and a humidity of 7〇% or less at room temperature -26-200942168, the air is forcibly circulated by the stirring fan to make it dry. (Test Zone 2) Test Zone 2 The above cut flowers were treated in the following procedure. 1. First substitution step: The immersion cut flower was immersed in the third kelp ethanol for 4 hours. φ 2·2nd substitution step: The cuts subjected to the first substitution step were immersed in the first mixed liquid (70% by volume of the third mixed ethanol, 30% by volume of the polypropylene glycol) for 3 hours. 3. Third substitution step: The cuts subjected to the second substitution step were immersed in the second mixed liquid (50% by volume of the third mixed ethanol, and the volume % of the polypropylene glycol) for 4 hours. 4. Fourth substitution step: The cuts subjected to the third substitution step were immersed in the third mixed liquid (30% by volume of the third mixed ethanol, 7% by volume of the polypropylene glycol) for 17 hours. 5. Washing step: The cut flower subjected to the above-mentioned fourth substitution step was washed with a third mixed ethanol for 30 seconds (three times for 10 seconds). 6_Drying step: The cutting step of performing the above-mentioned washing step is carried out in a drying chamber having a room temperature of 3 S ° C and a humidity of 70% or less, and the circulating air is forced to dry by a scramble fan. (Test Zone 3) The test zone 3 was subjected to the following steps to treat the cut flowers described above. -27- 200942168 1. The first substitution step: immersing the cut flowers in the first hour. 2. Second substitution step: The first flower was applied and immersed in the first mixed liquid (70% by volume of the third mixed % of polypropylene glycol) for 3 hours. 3. Third substitution step: The second flower was subjected to immersion in the second mixed liquid (polypropylene glycol of the third mixed % of 5% by volume) for 4 hours. 4. Fourth substitution step: The third flower was applied and immersed in the third mixed liquid (40% by volume of the third mixed % of polypropylene glycol) for 17 hours. 5. Washing step: the fourth step is performed by using the third mixed ethanol for 20 seconds (10 seconds washing 2 6. Drying step · · performing the above washing step to hold the room temperature at 3 5 ° C, drying chamber with a humidity of 70% or less!:| Circulating air to make it dry. (Test area 4) Test area 4 is to process the cut flowers according to the following procedure: 1. First substitution step: immersing the cut flowers in the first 2. Second substitution step: immersed in the first mixed solution (the third mixed % of polypropylene glycol in 7% by volume) for 3 hours by performing the above-mentioned second step. 3 Mixed ethanol in the 4 substitution step Cut ethanol, 30-capacity substitution step to cut ethanol, 50-capacity substitution step to cut ethanol, 60-capacity: cut flowers in the step, times). Cut flower, Yu Wei 3 'By the ethanol in a stirring fan strong 3 mixed ethanol, 4 substitution step, 30 capacity 200942168 3. Third substitution step: immersing the cut flower 'with the second substitution step described above' in the second mixture ( 40% by volume of table 3 mixed with ethanol, 60% by volume of polypropylene glycol) for 21 hours. 4. Washing step: The cut flower subjected to the third substitution step was washed with the third mixed ethanol for 20 seconds (washed twice for 10 seconds). 5. Drying step: The cut flower subjected to the above-mentioned washing step is stirred in a drying chamber at a room temperature of 35 ° C and a humidity of 70% or less, and the circulating air is dried. (Control Area) The control section was subjected to the following steps to treat the cut flowers described above. 1. First substitution step: immersing the cut flowers in the third mixed ethanol for 4 hours. 2. Second substitution step: The cuts subjected to the first substitution step were immersed in 100% by volume of polypropylene glycol for 15 hours. © 3. Washing step: Although the cut flower subjected to the second substitution step was washed with the third mixed ethanol, it could not be smoothly carried out. 4. Drying step: the cut flower subjected to the above washing step is subjected to a drying chamber at a room temperature of 35 ° C and a humidity of 7 % or less, and the air is forced to circulate and dried by a stirring fan. The results are as follows: 3 said. In addition, the symbols (+ and -) in Table 3 refer to the extent of the shape of the cut flower (dwarf, curl (anti-warping), shrinkage after drying), and the more +, the degree is large (that is, (1) < (+ - ) < ( + ) < ( + + ) < (+ + + )). -29- 200942168 (Results and review) In all the test areas of test areas 1 to 4, cut flowers were dwarfed and petal curls were less than control areas. Only test zone 3 is slightly shorter than the other test zones. The cleaning zone is the simplest in the test zone 1, and the test zone 2 costs 3 times the washing time of the test zone 1. Test zone 3 and test zone 4 require one more wash time than test zone 1.对照 The control area cannot be washed smoothly. From the above, it was found that any of the test zones 1 to 4 was easier to maintain the shape of the cut flower than the control zone (the conventional method of directly using the preservation solution), and also, for drying, the same strength as that of the control group was obtained. [Table 3] Test area dwarf curling washing time (seconds) Shrinkage after drying 1 - _ 10 + + 2 1〇χ3 _ 3 + - 10x2 4 - 10x2 + - Control area + + + + + + bad + + [ Industrial Applicability As described above, the present invention is effectively used for preserving cut flowers and manufacturing cut flowers, and is suitable for a cut flower shape which is stable for a long period of time. -30-