200916109 九、發明說明： 【發明所屬之技術領域】 本發明係關於-種胜肽躺產品，尤其是指—種從深海魚製得之機能性胜 肽、胜肽發酵物及其應用產品。 【先前技術】 裸鰛類（Myct〇Phidae)俗稱燈籠魚㈤temfishes)，主要分佈於斜中、深 水層的小独類’全球魏量料她絲數傾教間。這祕贿除部分 可供艮用外大π刀作為養殖用生辑，或製成魚粉和魚油，但因食用價值低又 容易腐敗，故讀為下誠紅棄㈣，不具經濟性，柄造減源浪 境污染等問題。 習知技術之機祕祖顧於域外舰成物祕健飲騎，個高成本之 胜肽來源’或在製造聰帽以萃取出高純度之概。料，傳紐肽因盆分 子量過大’造錢膚細胞魏效果不佳，甚至會糾發職反狀風險。而在 習知技術之添加難性紐之保健食品，亦因製造触，易導致產品帶有腫味 而造成消費者接受度不佳。 在贺1技術之抗菌劑或制_之活性成分多添加有機或化學藥劑，無論 在製以上或疋使用上，均有造成環境污染之虞。 =，謂合經赦益及職財的前提下，朗縣絲之天絲料製造 或健食品，以及製造出不添加有機或化學藥劑之抗菌 劑，實為目刚一重要的課題。 【發明内容】 為解決上關題，本發明倾供-觀海來源之機紐雜，其特徵在於， ，機能性職之來職直祕_舰行酵素轉师 分 量小於5000道耳。 >肝踯丑刀子 本發明亦提供-_絲源之該發酵物，具铸齡腐及除料一至多種 200916109 功能’該乳’酵㈣由深棘源之胜肽發酵祕’其特徵在於，該胜狀係源 自裸鰛類之酵素轉物，且分子4小於5_道耳，經 酸發酵物來作物k有赌性成分。 取雜肽礼 本發明亦提供-觀棘雜肽之細產品，為皮料驗·，包含機能 性胜肽及餘均質分散賴紐胜狀_ ’其碰在於，該機祕胜狀係源 自裸_讀素树物，且奸量掃5_道耳，轉域機紐祿作為活 化皮膚細胞之有效活性成分。 本發月亦提供種深海來源胜肽之應用產〇口口，為膠原蛋白合成促進劑，至少 包含有機紐概’其職纽，賴祕腿俩自裸㈣之騎水解物， 且分子量小於5000道耳，藉此促進膠原蛋白合成。 、 本發明亦提供—觀海來源胜肽之剌產品，為保健食品餘，至少包含有 機能性胜肽及可均冑錄職紐祖讀祕_，雜徵在於， ==__素轉物，且好量祕觸料，崎域機祕胜 肽促進抗體力泌而達到免疫調節之保健機能。 本發明亦提供-種深海來源胜肽之應用產品，為 能性胜肽及可均f分散該機能性胜肽之_，其特徵协，二有機 自裸_之酵素水解物，且分子量小於5_道耳 拖I胜肽係源 體分泌而_免疫·機能。 藉_機讀職促進抗 本發明亦提供-觀絲雜肽之顧產品 少包含有難難狀，料散職祕職除物質，至 性胜肽係源自裸_之轉轉物，取得分子量 徵在於，該機能 作為清除塌自自基之有效雜成分。 、G料之機紐胜肽來 本發明亦提供-觀海來義肽之顧產品 食品防腐劑，_在於，該_源自裸,^==；胜_製得之 觸道耳之舰性雌，㈣該發酵，取得魏發分子量小於 之有效活性齡。 輯麵料該食品防腐劑 200916109 本發明亦提供一種深海來源胜肽之應用產品，為深海來源之胜肽發酵製得 保健食品’其特徵在於，該胜肽係源自裸經類之酵素水解物，且分子量小於％〇〇 道耳之機能性胜肽，經過乳酸發酵，取得具有乳酸菌的可食性乳酸發酵物， 為該保健食品維護腸胃道機能之天然有效活性成分。 本發明亦提供一種深海來源胜肽之應用產品，為深海來源之胜肽發酵製得之 皮膚外用抑菌劑，其特徵在於，該胜肽係源自裸鰛類之酵素水解物，且分=量 小於5000道耳之機能性胜肽，經過乳酸發酵，取得乳酸發酵物來作為該^膚 用抑菌劑之有效活性成分。 因此，本發明之主要目的在於提供一種具有可提高裸魅附加價值、增加裸鰛 水產品競爭力之深海來源之機能性胜肽。 ‘ 小，易於吸收之深海來源 本發明之另一目的在於提供一種純度高，且分子量 之機能性胜肽。 本發明之另一目 能性胜肽。 的在於提供—種萃取_短且製造成本低之深絲源之機 ，本發明之另—目的在於提供—種具有可提高裸_加價值、增加裸财產品 競爭力之深海來源之乳酸發酵物。 易於吸收之深海 本發明之另一目的在於提供一種胜肽純度高，且分子量小， 來源之乳酸發酵物。 低之深海來源之乳 本發明之另—目的在於提供-種萃㈣職且製造成本 酸發酵物。 發/之3目的在於提供—種兼具食用安全性及㈣功能缝取代傳統 方腐劑，杜絕食品添加物對人體產生危害性之深海來源之乳酸發酵物。 以—目的在於提供—種可提高裸雜f加價值、增加裸财產品辭 力之深海來源胜肽之應用產品。 來源==的在於提供—種胜肽純度高’且分子量小，綱收之深海 200916109 本發明之另-目的在於提供_鮮取咖短且製造成本低之深絲源胜狀 之應用產品。 本發明之另一目的在於提供—種兼具食用安全性及抑菌功能而能取代傳統 防腐劑’轉食品添加娜讀私危紐之麟來聽肽之應用產品。 本發明之另-目的在毅供-射供人體個讀菌婦，並絲具維持消 化道正常之深海來源胜肽的保健應用產品。 【實施方式】 總體來說，本發明係利用捕撈所得之裸_，進行酵素水解，得到富含機能 性胜肽的酵素水解物。其中，裸_係、選自包含燈籠魚科在⑽多種深海备類， ==子量小於5_道耳，取得的胜肽可進-步作為皮膚外用組成 :質進劑、—^ 菌酵素水解物瞥步娜種乳酸8進行乳酸發酵，以取得具有抑 菌或4臭功％之乳酸發酵物，且該乳酸發酵物具有仙安全性、抗致突變性， 2天嫩咖咖。再者，树犧綱_嫩發酵為商業 上常用乳_，、軌__含量符麵齡品要求含量每毫升欧阳以上， 可再進-步作為食品防細，以及健胃整制途的保健食品。 因此，以下配合各實施例分別說明本發明之深絲源 源胜狀之皮斜驗成物、騎、蛋自合雜·、節免疫雜之來 人^由基清除純’或者進—步提供包纽深絲雜狀經乳 酸發酵產生之狀發酵物以及以乳酸發酵物作於皮膚外用抑 H或以及各比較例中所採用之實驗程序步驟所利用之基本原理與基本架 完整：=域具有通常知識者所能明瞭，下文+之說明，不再作 200916109 第一實施例（深海來源之機能性胜肽） 本發明之第一實施例係揭示一種深海來源之機能性胜肽’該機能性胜肽係將 捕撈所得之裸鰛類直接加以酵素水解，待酵素水解完成，即取得富含機能性胜 肽之酵素水解液。該機能性胜肽具有活化皮膚細胞、促進膠原蛋白合成、活化 免疫細胞調節及清除人體自由基等功能。 其中，機能性胜肽之裸鰛類來源係選自底燈魚屬（Benthosemaspp.)、眶燈 魚屬（Diaphusspp.)、珍燈魚屬（Lampanyctusspp.)、角燈魚屬（Ceratoscoplus spp.)、炬燈魚屬（Lampadenaspp.)、背燈魚屬（Notoscopelusspp.)、標燈魚 屬（Symbolophrus spp.)、臂燈魚屬（Stenobrachius spp.)、燈籠魚屬（Myctophum spp.)及所組之組群。 經酵素水解所得之機能性胜肽，如七胜肽、六胜肽或五胜肽，分子量小於 5000道耳，甚至較佳以分子量小於3〇〇〇道耳之胜肽為收集對象，取得純度高於 90%以上之機能性胜肽。由於分子越小，吸收之速度越快，該機能性胜肽之分子 量較佳分佈範圍為小於3000道耳，而最佳範圍係為介於2〇〇至1〇〇〇道耳。 第二實施例（深海來源胜肽之皮膚外用組成物） 本發明之第二實施_為-種含有縣來雜肽之皮膚外驗餘，該皮膚 外用組成物之_ ’包含有取得自裸賴之機紐胜肽及均質分散該機能 性胜肽之細。取得該皮斜用組成物之機紐雜步驟，如第丨圖流程示意 圖所示，首先_騎狀__接加轉素水解，御素轉減，取得 富含機能餘狀酵素水舰，之後餅轉素水聽進行顏倾，以使得 將來製成之皮斜醜成物，额因為含有麵為飾生物之機祕胜肽而具 有腥臭味，藉此提高消費者使用_。最後脫腥完畢，取得該皮膚外用組成物 活性成分。 其中’該機能性胜肽之裸鰛類來源係選自底燈魚屬（Benth_aspp )、眶 燈魚屬（Diaphusspp·)、珍燈魚屬（L—零如鄉.）、練魚屬⑽鄭⑺卿 200916109 spp.)、炬燈魚屬（Lampadenaspp.)、背燈魚屬（Notoscopelusspp.)、標燈魚 屬（Symbolophrus spp.)、臂燈魚屬（Stenobrachius spp.)、燈籠魚屬（Myctophum spp.)及所組之組群。 經酵素水解所得之機能性胜肽，如七胜肽、六胜肽或五胜肽，分子量小於 5000道耳，甚至較佳以分子量小於3000道耳之胜肽為收集對象，取得純度高於 90%以上，且可有效活化皮膚真皮層細胞之機能性胜肽。由於分子越小，皮膚吸 收之速度越快，也越能滲透到真皮層，達到深層滋潤的效果，所以該機能性胜 肽之分子量較佳分佈範圍為小於3000道耳，而最佳範圍係為介於2〇〇至1〇〇〇 道耳。 比較例一 比較例一係分別從第2A圖及第2B圖比較本發明之第一實施例中取得深海 來源之機能性胜肽與對照組之活化皮膚表層細胞及真皮層細胞當中，纖維母細 胞（第2A圖）及角質細胞（第2A圖）之效益。 首先，參閲第2A圖所示，在經由酵素水解後取得裸鰛類之機能性胜肽，以 不同濃度（0.025mg/ml、0.05mg/ml、0. lmg/m卜 〇. 19mg/m卜 〇 37mg/ml、〇 74mg/ml、 lfmg/m卜2.94mg/mb 5.88mg/ml)分別加入纖維母細胞（fibr〇blast)之培養液 中’觀察其活化纖維母細胞生長之效果，並與一對照組比較。其結果如第2A圖 所示，當對照組活化纖維母細胞生長效果為1〇〇%時，經由酵素水解所得之裸鰛 類之機能性胜狀活化纖維母細胞生長之效果最高為胤2%，顯見本發明之深海 來源之機能性胜肽可有效活化皮膚真皮層之纖維母細胞。 再而，如第2B圖所示，以相同方式觀察在經由酵素水解後取得裸經類之機 能性胜肽，以不同濃度（〇.12mg/m卜 〇.23mg/m卜 〇45mg/ml、〇 89mg/ml、 1.77mg/m卜3.53mg/m卜7.06mg/ml)分別加人含有肖質細胞（keratin〇响）之 培養液中’活化角質細胞生長之效果’其結果如第2B圖所示，當對照組活化角 質細胞生長效果為腦％時，經由酵素水解所得之裸賴之機能性胜肽活化角質 11 200916109 細胞生長之效果最高為153.5%，顯見本發明之深海來源之機能性胜肽可有效促 進表皮層細胞活性。 綜上所述，本發明由裸鰛類酵素水解取得之機能性胜肽，可有效活化皮膚 表皮層及真皮層細胞。 第三實施例（深海來源胜肽之膠原蛋白合成促進劑） 本發明第三實施例係揭露一種膠原蛋白合成促進劑，具有改善皮膚粗糙度的 效果及提咼肌膚含水量的功能，可供外敷或内服使用，該膠原蛋白合成促進劑， 係含有自裸經類經由酵素水解後，分子量小於5〇〇〇道耳之機能性胜肽，並且在 酵素水解後可以進一步實施脫腥步驟。惟酵素水解及脫腥步驟同於第二實施例 中所述，首先係將捕撈所得之裸鰛類直接加以酵素水解，待酵素水解完成即取 得富含機雛雖之酵素水驗，之後縣鱗素水舰骑脫齡驟，以取 得該具有促進膠原蛋白合成功能之機能性胜肽。 本實施例不同於刖案之處，主要在於機能性胜肽係自裸經類取得，裸經類係 來源係選自底燈魚屬（Benthosemaspp.)、眶燈魚屬（Diaphusspp.)、珍燈魚 屬（Lampanyctusspp.)、角燈魚屬（Cerat〇se〇plusspp )、炬燈魚屬㈤率也皿 spp.)、背燈魚屬（Notoscopelusspp.)、標燈魚屬（Symbolophrusspp.)、臂燈 魚屬（Stenobrachiusspp.)、燈籠魚屬（Myctophumspp )及所組之組群。經酵 素水解取得純度高於90%以上之機能性胜肽，例如七胜肽、六胜肽或五胜狀以 及其他寡胜肽（Oligopeptides) ’本實施例係以分子量小於5_道耳為收集對 象，尤其分子越小，人體吸收速度越快，故該機能性胜肽之分子量較佳分佈範 圍為小於3000道耳，而最佳範圍係為介於2〇〇至1〇〇〇道耳。 比較例二 比較例二係比較本發明之第三實施例中深海來源之機能性胜肽與 進膠原蛋白合成作用之差異程度。 ~ 12 200916109 如第3A圖所不，經由酵素水解取得裸鰛類深海魚之機能性胜肽，以不同濃 Jk ( .23 g/ml 0.45mg/ml > 0.89mg/mi , i.77mg/ml > 3.53mg/ml' 7.06mg/ml) :別/則试其促進σ成膠原蛋白之效果，並與一對照組比較。其結果如第从圖所 示，當對照組之膠原蛋白合成促進作用為腦時，經由酵素水解之裸經類深海 魚機能性祖對膠原蛋自合成促進作用最高為⑼州。 又’從第3Β圖、第3C圖、第3D圖及第3Ε圖結果可知，從人體試驗觀察 受試者使聰《貞之機雛胜轉素水解液15分鐘前後，在麵、右眼尾和左 臉頰等不同皮膚部位之彈力、緊實度、經皮水分散失、表皮水分含量差異，發 現到受試者普遍皆有7%〜㈣的改善率，顯示裸絲源之機能性胜肽確實具有 除皺、保濕和緊實的效果。 綜合上述結果顯見本發明之深海來源機能性胜肽可有效促進勝原蛋白之合 成’並具有改善皮膚粗驗的效果及提高肌膚含水量的功能。 第四實施例（具免疫調節機能之保健食品素材） 本發明第四實施例係揭露一種具免疫調節機能之保健食品素材，至少包含有 機能性胜狀可均f分散賴雛雖之食祕麵，機紐紐係自裸經類 經由酵素轉後轉分子量祕5_道耳之職，其麵係直接賴撈所得之 裸經類加以酵素轉，獅素水解完成，取得f含機祕馳之酵素水解液， 即獲得具有免疫調節機能之機能性胜肽。 本實施例不同於前案之處，主要在於機能性胜肽係自裸鰛類取得，裸鰛類來 源係選自底燈魚屬（Benthosemaspp )、眶燈魚屬（Diaphusspp.)、珍燈魚屬 (Lampanyctusspp.)、角燈魚屬（ceratoscoplusspp·)、炬燈魚屬（Lampadena SPP·)、背燈魚屬（Notoscopelusspp.)、標燈魚屬（Symbolophrusspp.)、臂燈 魚屬（Stenobrachiusspp.)、燈籠魚屬（Myctophumspp.)及所組之組群。經酵 素水解取得純度高於90%以上之機能性胜肽，例如七胜肽、六胜肽或五胜肽以 及其他寡胜肽（Oligopeptides) ’本實施例係以分子量小於5000道耳為收集對 13 200916109 象’尤其分子越小，人體吸收速度越快，故該機能性胜肽之分子量較佳分佈範 圍為小於3_道耳’而最佳範圍係為介於雇^^⑻道耳。而食用性載劑也 可進-步包括動物膠、海轉、中草藥萃取液、明膠、澱粉、水或其他相分散 物質。 第五實施例（深海來源胜肽之免疫調節劑） 本發明第五實施例係揭露一種深海來源胜肽之免疫調節劑，該免疫調節劑之 組成係包含自裸鰛類經由酵素水解後取得之機能性胜肽以及可均質分散該機能 性胜肽之載劑，其中，取得該機能性胜肽之酵素水解步驟及該機能性胜肽係出 之於裸鰛類酵素水解物等說明，均同於第一實施例中所述，在此不再重複贅述。 經由酵素水解所收集到之該機能性胜肽分子量小於5〇〇〇道耳，且取得之機 能性胜肽純度極高，並可作為有效促進抗體分泌之免疫調節有效活性成分。並 且，為達到讓使用者能夠有效吸收，該機能性胜肽之分子量分佈範圍較佳為小 於3000道耳，而最佳範圍係為介於2〇〇至1〇〇〇道耳。 比較例三 比較例三係比較本發明之第四及第五實施例所述，比較取自深海來源之機能 性胜肽與對照組在活化免疫細胞並促進抗體分泌之差異程度。 如第4A圖及第4B圖所示，本發明在經由酵素水解取得裸鰛類深海魚之機 能性胜肽’以不同濃度（〇.2mg/ml、0.4mg/ml、0.79mg/ml、1.57mg/ml、3.14mg/ml、 6.28mg/ml)分別加入免疫細胞（hb4C5)之培養液中，觀察其活化免疫細胞生 長之效果，並分析免疫細胞IgM抗體之分泌量，與一對照組比較。第4A圖為 活化免疫細胞HB4C5生長效果，第4B圖為免疫細胞HB4C5受到該機能性胜肽 活化後IgM之分泌量。其中，當對照組活化免疫細胞HB4C5生長效果為100% 時’經由酵素水解所得之裸鰛類機能性胜肽活化免疫細胞HB4C5生長之效果最 高為134% ;而當對照組活化免疫細胞HB4C5後IgM之分泌量為100%時，經 200916109 由酵素水解所得之裸鰛類機能性胜肽活化免疫細胞之分泌量最高 為122% ’顯見本發明之深海來源之機能性胜肽可有效活化免疫細胞並促進抗體 分泌，達到免疫調節之功能。 第六實施例（深海來源之人體自由基清除物質） 本發明第六實施例係揭露一種深海來源且可清除細胞周圍之自由基，免除自 由基傷害和增舰細胞抗氧化能力之人體自由基清除物f，此人體自由基清除 物質可單獨使贼·其他可食性麵或—般皮料用細共_發成保健飲 食品、絲品或抗氧化安定劑。其巾，人體自由基清除物f係含有自裸經類經 由酵素水賴取得好量小於5GGG道耳之機能,賺肽，其巾酵素水解及脫腥步 驟同於第二實施例帽述’首先係直接將捕撈所得之裸_加鱗素水解，待 酵素水解完成’取得f含機紐職之酵素水驗，之後再將鱗素水解液進 行脫腿步驟，消_臭麵取得财有絲自自基魏之機祕雖，該機能 性胜肽能作為清除自由基之有效活性成分。 本實施例不同於前案之處，主要在於機能性胜肽係取得自裸鰛類，其來源係 選自底燈魚屬（Benthosemaspp.)、眶燈魚屬（Diaphusspp )、珍燈魚屬 (Lamp卿ctusspp.)、角燈魚屬（Cerat〇sc〇plusspp )、炬燈魚屬㈤寧如⑽ 鄉.）、背燈魚屬（N〇t〇SC〇Pdusspp·)、標燈魚屬（Symb〇1〇phmsspp )、臂燈 魚屬（Stenobmchiusspp.)、燈籠魚屬（Myct〇phumspp )及所組之組群。萃取 後經酵素水解取得純度高於9G%以上之機能性胜肽，例如七胜肽、六胜狀或五 胜肽以及其他寡胜肽（〇ligopeptides)，本實施例係以分子量小於5〇〇〇道耳為 收集對象，尤其分子越小，人體魏速度触，故職雛祖之分子量較佳 分佈範圍為小於3000道耳，而最佳範圍係為介於2〇〇至1〇〇〇道耳。 比較例四 比較例四係比較本發明之第六實施例中取得之機能性胜肽與對照組清除自 15 200916109 由基之活性之差異程度。 在經由酵素水解及脫腥步驟以後，取得裸鰛類機能性胜肽，分析其清除 2,2-diphenyl-l-piCrylhydrazyl (DPPH)自由基之能力（DppH 清除力）及螯合鐵 能力’且因已知維他命C為一具有強抗氧化力及能有效清除自由基之化合物， 而EDTA為一已知具有強離子螯合力之抗氧化化合物，故分別以2〇〇ppm之維 他命C以及20〇ppm之EDTA作為正對照組（抗氧化效力1〇0%)，藉此比較該 機能性胜肽清除自由基以及抗氧化之程度。 結果如表一所示’當2〇〇ppm的維他命C之DPPH清除力為95.18±0.62°/〇時， 經由酵素水解再脫腥步驟所得之裸鰛類深海機能性胜肽之DppH清除力為 93.88±0.66% ’與正對照組之DPPH清除力相當（為正對照組維他命c之97°/〇); 而同時200ppm之EDTA螯合鐵能力為99.76±0.12%時，經由酵素水解再脫腥步 驟所得之裸鰛類深海機能性胜肽之螯合鐵能力為85 %±3 56%，亦與正對照組相 去不遠（為正對照組EDTA之84%)，顯見本發明之深海來源機能性胜肽能有 效清除自由基及具有良好抗氧化能力。 一深海來源機能性胜肽輿掛照紕之浩除自由基能力 樣品名稱 VIT.C (200ppm) EDTA (200ppm) 裸鰛深海胜肽 DPPH清除力（％)螯合鐵能力（。/〇) 95.18±0.62 99.76±0.12 93.88±0.66 85.96±3.56 第七實施例（深海來源之乳酸發酵物） 本毛月之第七實施例係為一種深海來源之乳酸發酵物，係由裸經類經過酵素 水解取得讀紐雜’再湘級贿紐得。該乳酸俩物具有抑菌功能， 可作為肌m制®之雜有效成份，或加碰供作食品相之喊劑或是搭 配乳酸菌一同作為天然保健食品之活性有效成份。 該胜肽來源之乳酸發酵物取得步驟如同第5圖萃取流程示意圖所示，首先係 16 200916109 將捕撈所得之裸經類加以酵素水解。待水解完成後取得富含機能性胜肽之酵素 水解液，再將酵素水解液進行乳酸發酵，取得乳酸發酵產物。其中，進行乳酸 發酵所使用之菌種’係選自乳酸桿菌屬（Lactobacillus spp.)、乳酸球菌屬 (Lactococcus spp.)、足球菌屬（Pediococcus spp.)、鏈球菌屬（streptococcus spp.) 及雙歧桿菌屬（Bifidobacterium spp·)及所組之組群。 此外，該機能性胜肽係酵素水解自裸鰛類，此裸鰛類來源係選自底燈魚屬 (Benthosemaspp·)、眶燈魚屬（Diaphusspp.)、珍燈魚屬（Lampanyctusspp )、 角燈魚屬（Ceratoscoplusspp.)、炬燈魚屬（Lampadenaspp.)、背燈魚屬 (Notoscopelusspp.)、標燈魚屬（Symb〇i〇phrusspp )、臂燈魚屬（Sten〇brachius Spp.)、燈籠魚屬（Myctophumspp.)及所組之組群。 第八實施例（深海來源之皮膚外用抑菌劑） 本發明之第八實施例係為一種含有裸鰛類來源乳酸發酵物之皮膚外用抑菌 劑，該裸經類來源之皮膚外用抑菌劑係包含有由裸I显類經酵素水解後取得之機 能性胜肽，經過乳酸發酵，得到具有抑菌效果之乳酸發酵物，以及可均質分散 該乳酸發酵物之載劑，可以有效抑制上皮鏈球菌（staphylococcusediperalidis) 及痤瘡桿菌（Propicnibateriumacnes)等體表菌叢，達成肌膚清潔制菌之目的。 該裸鰛類來源之乳酸發酵物質，取得步驟如前述之第七實施例中第5圖萃取 流程示意圖所示，首先係將捕撈所得之裸鰛類加以酵素水解。待水解完成後取 得画含機能性胜肽之酵素水解液，再將酵素水解液進行乳酸發酵，最後以取得 具有抑菌功能之乳酸發酵物。其中，進行乳酸發酵所使用之菌種，係選自乳酸 才干菌屬（Lactobacillus spp.)、乳酸球菌屬（Lactococcus spp.)、足球菌屬 (Pediococcus spp.)、鏈球菌屬（strept〇coccus Spp.)，及雙歧桿菌屬 (Bifidobacterium spp.)及所組之組群，而該機能性胜肽之來源係取得自裸鰛 類’來源係選自底燈魚屬（Benth〇semaspp.)、眶燈魚屬（Diaphusspp.)、珍 燈魚屬（Lampanyctus spp.)、角燈魚屬（Ceratosc〇plus spp )、炬燈魚屬心寧如⑽ 17 200916109 SPP.)、背燈魚屬（Notoscopelusspp.)、標燈魚屬（Symbolophrusspp.)、臂燈 魚屬（Stenobrachiusspp.)、燈籠魚屬（Myctophumspp.)及所組之組群。 此外，本實施例中所取得之具有抑菌功能之乳酸發酵物製成之皮膚外用抑菌 劑’可進一步製成具有消臭功能之皮膚外用消臭劑。 比較例五 比較例五係顯示本發明之第七實施例、第八實施例及以下第九及第十實施 例中’裸經類來源之機能性胜肽經由乳酸發酵後產出的乳酸發酵物，與對照組 相較’在李斯特菌（Listeria monocytogenes)和痤瘡桿菌（Propicnibaterium acnes ) 之抑菌結果。其中李斯特菌係為造成食物污染之一常見菌種，而痤瘡桿菌則為 一誘發皮膚產生痤瘡之主要菌種。 如表二所示，裸鰛類來源之機能性胜肽乳酸發酵後產出的乳酸發酵物，對 於李斯特菌的抑菌狀況，與對照組MRS基礎培養基相較，抑菌圈直徑由〇 9公 分提南為1.0公分；對於痤瘡桿菌的抑菌功效，與對照組基礎培養基相較， 抑菌圈直獅I.7公分提高為1.9公分。顯見裸麵來狀機紐胜肽乳酸發酵 物，對李斯特菌和痤瘡桿菌具有顯著的抑菌功效，故可作為皮膚外用抑菌劑及 食品防腐或保健食品之活性有效成份。 表二裸經類來源乳酸發酵物與對照組之抑菌能为 抑制圈（公分) 李菌 1.0 座瘡桿菌 L9 裸線水解液 λ/TOQ a 〇200916109 IX. DESCRIPTION OF THE INVENTION: TECHNICAL FIELD OF THE INVENTION The present invention relates to a peptide-like product, and more particularly to a functional peptide, a peptide fermentation product, and an application product thereof, which are prepared from deep-sea fish. [Prior Art] The naked scorpion (Myct〇Phidae) is commonly known as the ternal fish (five) temfishes, which are mainly distributed in the middle and middle of the sloping middle and deep water layers. In addition to some of the secret bribes, the large π knives can be used as breeding breeding series, or made into fishmeal and fish oil, but because of low food value and easy to be corrupt, it is read as a sincere red (four), not economical, handle made Issues such as reducing source pollution. The secret tactics of the know-how are based on the extraterrestrial ship's secret health drink, a high-cost source of peptides, or the manufacture of Cong Cap to extract high purity. It is expected that the amount of the new peptide will be too large due to the large amount of potted molecules. However, the health foods that are added to the difficulties of the prior art are also caused by the manufacturing touch, which may cause the product to have a swollen taste and cause poor consumer acceptance. Adding organic or chemical agents to the antibacterial agent or the active ingredient of the Heyi technology has caused environmental pollution regardless of the above or above. Under the premise of cooperating with the benefits and occupational wealth, Langxian Silk's silk fabric manufacturing or health food, as well as the production of antibacterial agents without the addition of organic or chemical agents, is an important issue. SUMMARY OF THE INVENTION In order to solve the above problem, the present invention provides a source of opportunity for the source of the sea, which is characterized by the fact that the function of the functional job is less than 5,000 ears. >Liver ugly knife The present invention also provides - _ silk source of the fermented product, with cast age rot and remove material one to more 200916109 function 'the milk' leaven (four) from the deep spine of the peptide fermentation secret 'character The trait is derived from the enzyme transfer of the naked mites, and the molecule 4 is less than 5 _ ears, and the crop k has a gambling component by the acid ferment. The invention also provides a fine product of the thorn-like peptide, which is a skin test, and contains a functional peptide and a heterogeneous dispersion of lysine _ _ 'the impact is that the machine is derived from Naked _ read the tree, and the amount of rape swept 5_ ear, the domain machine Nero as an effective active ingredient for the activation of skin cells. This month also provides the application of a deep-sea source peptide. It is a collagen synthesis promoter, which contains at least the organic New Zealand's job, and the two legs from the bare (four) ride hydrolysate, and the molecular weight is less than 5000. The ear can thereby promote collagen synthesis. The present invention also provides a product of the sea-derived peptide, which is a health food, contains at least an organic energy peptide, and can be uniformly recorded in the New Zealand reading secret _, the miscellaneous is, ==__ And a good amount of secret touch, Kasumi machine secret peptides promote antibody secretion and achieve the immune function of immune regulation. The invention also provides an application product of a deep-sea source peptide, which is an energy peptide and can uniformly disperse the functional peptide, and its characteristic association, two organic self-naked enzyme hydrolysate, and the molecular weight is less than 5 _ Daoer drag I win peptide source secretion and _ immunity and function. Borrowing the machine to promote the anti-the present invention also provides - the observation of the silky peptides contains less difficult materials, the material is secretly secreted in addition to the substance, the sex peptide is derived from the naked _ turn, obtain the molecular weight sign Therefore, the function can be used as an effective impurity component for removing collapse from the base. , G material machine Newkin peptide to the present invention also provides - Guanhailaiyi peptide product food preservative, _ lie, the _ from the naked, ^ = =; win _ made the touch of the ear of the ship Female, (d) the fermentation, the Weifei molecular weight is less than the effective age of the active. Fabrics, the food preservative 200916109 The present invention also provides an application product of a deep-sea source peptide, which is obtained by fermenting a peptide derived from deep-sea sources, wherein the peptide is derived from an enzyme hydrolyzate of a naked body, and The functional peptide having a molecular weight of less than % 〇〇 耳,, after lactic acid fermentation, obtains an edible lactic acid fermented product having lactic acid bacteria, and maintains a natural effective active ingredient of gastrointestinal function for the health food. The invention also provides an application product for a deep-sea source peptide, which is a skin external bacteriostatic agent prepared by fermentation of a peptide derived from deep sea, characterized in that the peptide is derived from an enzyme hydrolyzate of naked steroids, and the sub- = The functional peptide is less than 5,000 ears, and after lactic acid fermentation, a lactic acid fermented product is obtained as an effective active ingredient of the bacteriostatic agent for the skin. Accordingly, it is a primary object of the present invention to provide a functional peptide which is of deep sea origin which can increase the added value of the naked charm and increase the competitiveness of the naked fish. ‘Small, readily absorbable deep sea source Another object of the present invention is to provide a functional peptide having a high purity and a molecular weight. Another achievable peptide of the present invention. It is to provide a deep-filament source with short extraction and low manufacturing cost, and another object of the present invention is to provide a deep-sea source lactic acid fermentation product which can improve the nakedness and increase the competitiveness of naked products. . Deep sea which is easy to absorb Another object of the present invention is to provide a lactic acid fermented product which has a high purity of a peptide and a small molecular weight. Low Deep Sea Source Milk Another aspect of the present invention is to provide an acid fermentation product that is produced in a four-part (IV) position. The purpose of the hair / 3 is to provide a kind of deep-sea source lactic acid fermented product that has both food safety and (4) functional seams instead of traditional square rotants to prevent food additives from harming the human body. In order to provide an application product for deep-sea source peptides that can increase the value of naked impurities and increase the reputation of naked products. The source== is to provide a high purity of the peptide, and the molecular weight is small, and the deep sea of the invention is 200916109. Another object of the present invention is to provide an application product which is short-lived and has a low manufacturing cost. Another object of the present invention is to provide an application product which has both food safety and bacteriostatic functions and can replace the traditional preservatives. Another object of the present invention is to provide a health care application product for the deep-sea source peptide which maintains the normalization of the digestive tract. [Embodiment] In general, the present invention utilizes the nakedness obtained by fishing to carry out enzymatic hydrolysis to obtain an enzyme hydrolyzate rich in functional peptides. Among them, the naked _ series, selected from the genus Lanternidae in (10) a variety of deep-sea preparations, == sub-quantity less than 5 _ ears, the obtained peptide can be further into the skin for external use: quality agent, - ^ bacteria enzyme The hydrolyzate 瞥 娜 种 lactic acid 8 is subjected to lactic acid fermentation to obtain a lactic acid fermented product having bacteriostatic or odor-producing activity, and the lactic acid fermented product has safety and anti-mutagenicity, and is tempered for 2 days. In addition, the tree syllabus _ tender fermentation is commonly used in commercial milk _,, _ _ content of the age of the product, the required content per ml of Ouyang, can be further step-by-step as a food anti-fine, and health care system food. Therefore, the following is a description of the skin of the deep-sourced source of the present invention, the ride, the egg, the self-mixing, the section of the immune system, and the cleaning of the pure or the step-by-step package. The basic principle and basic frame of the fermentation product produced by lactic acid fermentation and the lactic acid fermentation product for external use of the skin or the experimental procedure used in each comparative example: the domain has the usual The knowledge of the knowledge, as explained in the following +, is no longer the first embodiment of 200916109 (the functional peptide of deep-sea source). The first embodiment of the present invention reveals a functional peptide of deep-sea source, which is superior to the function. The peptide system directly hydrolyzes the naked mites obtained by the harvesting, and after the hydrolysis of the enzyme is completed, the enzyme hydrolyzate rich in functional peptides is obtained. The functional peptide has the functions of activating skin cells, promoting collagen synthesis, activating immune cell regulation, and eliminating free radicals in the human body. Among them, the source of the naked scorpion of the functional peptide is selected from the group consisting of Benthosemaspp., Diaphusspp., Lampanyctusspp., and Ceratoscoplus spp. , Lampadenaspp., Notoscopelusspp., Symblophus spp., Stenobrachius spp., Myctophum spp. Group of groups. A functional peptide obtained by hydrolysis of an enzyme, such as a seven-peptide, a six-peptide or a five-peptide, having a molecular weight of less than 5,000 ears, or even preferably a peptide having a molecular weight of less than 3 angstroms as a collection object, obtaining purity More than 90% of the functional peptides. The smaller the molecule, the faster the absorption rate. The molecular weight of the functional peptide is preferably less than 3000 amps, and the optimal range is between 2 〇〇 and 1 〇〇〇. SECOND EMBODIMENT (Skin-derived composition of deep-sea source peptide) The second embodiment of the present invention is a skin-external test containing a heteropeptide of the county, and the external composition of the skin contains the obtained from the naked The machine is a peptide and homogenizes the fineness of the functional peptide. Obtaining the machine-mixing step of the composition for the skin obliqueity, as shown in the schematic diagram of the flow chart of the first drawing, firstly, the _ riding __ is coupled with the transfer of the hydrolysate, and the sulphate is reduced, and the water-rich enzyme water ship is obtained. The cake is transferred to the water to listen to the color, so that the skin made in the future will be ugly, and the amount will be odor-causing because it contains the surface of the creature. Finally, the dislocation is completed, and the active ingredient of the external composition for the skin is obtained. Among them, the source of the naked scorpion of the functional peptide is selected from the group consisting of Benth_aspp, Diaphusspp, and the genus (L-Zuo Ruxiang), and the genus (10) Zheng. (7)Qing 200916109 spp.), Lampadenaspp., Notoscopelusspp., Symblophus spp., Stenobrachius spp., Myctophum Spp.) and the group of groups. A functional peptide obtained by hydrolysis of an enzyme, such as a seven-peptide, a six-peptide or a five-peptide, having a molecular weight of less than 5,000 ears, or even preferably a peptide having a molecular weight of less than 3,000 ears, is obtained as a collection object, and a purity higher than 90 is obtained. More than %, and can effectively activate the functional peptide of the skin dermis cells. The smaller the molecule, the faster the skin absorbs, and the more it penetrates into the dermis layer, the deeper the moisturizing effect. Therefore, the molecular weight of the functional peptide is better distributed in the range of less than 3000 ears, and the optimal range is Between 2 and 1 track. Comparative Example 1 Comparative Example 1 is a comparison of the first embodiment of the present invention from the second embodiment of FIG. 2 to the second embodiment of the present invention to obtain fibroblasts from the activated skin surface layer cells and dermis cells of the deep-sea source functional peptide and the control group. (Fig. 2A) and the benefits of keratinocytes (Fig. 2A). First, as shown in Fig. 2A, after the hydrolysis of the enzyme, the functional peptides of the naked mites are obtained at different concentrations (0.025 mg/ml, 0.05 mg/ml, 0.1 mg/m dip. 19 mg/m). Obesity 37mg/ml, 〇74mg/ml, lfmg/m b 2.94mg/mb 5.88mg/ml) were added to the fibroblasts (fibr〇blast) culture medium respectively to observe the effect of the growth of activated fibroblasts, and Compare with a control group. The results are shown in Fig. 2A. When the growth factor of the activated fibroblasts in the control group is 1%, the effect of the growth of the naked mites on the activated fibroblasts obtained by the hydrolysis of the enzyme is up to 胤2%. It is apparent that the functional peptide of deep sea origin of the present invention can effectively activate fibroblasts of the dermis layer of the skin. Further, as shown in Fig. 2B, in the same manner, the functional peptides of the naked genus were obtained after hydrolysis by the enzyme at different concentrations (〇.12 mg/m 〇.23 mg/m 〇45 mg/ml, 〇 89mg/ml, 1.77mg/m, 3.53mg/m, and 7.06mg/ml) respectively, the effect of 'activated keratinocyte growth' in the culture medium containing erythrocyte (keratin) was obtained as shown in Fig. 2B. It is shown that when the growth keratinocyte growth effect of the control group is brain%, the effect of the growth of the keratin 11 keratin 11 200916109 cells obtained by the hydrolysis of the enzyme is up to 153.5%, and it is apparent that the function of the deep sea source of the present invention is superior. Peptides are effective in promoting epidermal cell activity. In summary, the functional peptide obtained by the hydrolysis of the naked cockroach enzyme of the present invention can effectively activate the epidermal layer and the dermis layer of the skin. Third Embodiment (Collagen Synthetic Promoter of Deep Sea Source Peptide) The third embodiment of the present invention discloses a collagen synthesis accelerating agent which has the effects of improving skin roughness and improving the moisture content of the skin, and can be externally applied. Or, for internal use, the collagen synthesis promoter contains a functional peptide having a molecular weight of less than 5 ampoules after hydrolysis by an enzyme via an open bacterium, and the desorption step can be further performed after the enzyme is hydrolyzed. However, the hydrolysis and desorption steps of the enzyme are the same as those described in the second embodiment. First, the naked mites obtained by the fishing are directly hydrolyzed by the enzyme, and after the hydrolysis of the enzyme is completed, the enzymatic water test enriched in the machine is obtained, and then the scale is obtained. The water tanker rides off the age to obtain the functional peptide that promotes the synthesis of collagen. This embodiment is different from the case, mainly because the functional peptide is obtained from the naked meridian, and the source of the naked genus is selected from the group consisting of Benthosemaspp., Diaphusspp., and Jane. Genus (Lampanyctusspp.), Cape genus (Cerat〇se〇plusspp), Tortoisefish (5) rate also dish spp.), genus (Notoscopelusspp.), genus (Symbolophrusspp.), arm light Fish (Stenobrachiusspp.), Lantern (Myctophumspp) and the group of the group. Functional peptides with a purity greater than 90% by enzyme hydrolysis, such as seven peptides, six peptides or five wins, and other Oligopeptides. This example is based on a molecular weight of less than 5 Å. The object, especially the smaller the molecule, the faster the absorption rate of the human body, so the molecular weight of the functional peptide is preferably distributed in the range of less than 3000 ears, and the optimal range is between 2 〇〇 and 1 〇〇〇. Comparative Example 2 Comparative Example 2 compares the degree of difference between the functional peptides of the deep-sea source and the collagen synthesis in the third embodiment of the present invention. ~ 12 200916109 As shown in Figure 3A, the functional peptides of the naked squid deep sea fish were obtained by enzyme hydrolysis, with different concentrations of Jk (.23 g/ml 0.45 mg/ml > 0.89 mg/mi, i.77 mg/ Ml > 3.53mg/ml' 7.06mg/ml): Do not try to promote the effect of σ into collagen and compare it with a control group. The results are shown in the figure. When the collagen synthesis promoting effect of the control group is brain, the functional activity of the naked-sea deep-sea fish functional ancestors hydrolyzed by the enzyme promotes the self-synthesis of collagen eggs to the highest (9) state. 'From the results of the 3rd, 3rd, 3D, and 3rd drawings, it can be seen from the human body test that the subject is convinced that the cockroach cockroach wins the liquid hydrolysate for 15 minutes before and after the face and right eye and The elasticity, firmness, transepidermal water loss and epidermal moisture content of different skin parts such as the left cheek were found to have a 7% to (four) improvement rate in the subjects, indicating that the functional peptide of the naked silk source does have Wrinkle, moisturizing and firming effect. Based on the above results, it is apparent that the deep-sea source functional peptide of the present invention can effectively promote the synthesis of the original protein and has the functions of improving the skin roughness and improving the skin moisture content. The fourth embodiment (health food material with immunomodulating function) The fourth embodiment of the present invention discloses a health food material with immunomodulating function, which at least contains organic energy and can be dispersed. The machine button is transferred from the naked meridian to the molecular weight of the 5th ear by the enzyme. The surface of the machine is directly transferred to the naked meridian obtained by the enzyme, and the enzyme is hydrolyzed to obtain the enzyme hydrolysis of the f-containing machine. Liquid, that is, to obtain a functional peptide with immunomodulatory function. This embodiment differs from the previous case in that the functional peptide is obtained from the naked mites, and the source of the naked mites is selected from the group consisting of Benthosemaspp, Diaphusspp., and Jane. Genus (Lampanyctusspp.), genus ceratoscoplusspp, Lampadena SPP·, Notoscopelusspp., Symblophus spp., Stenobrachiusspp. ), the genus of the genus Myctophumspp. and the group of the group. Functional peptides with a purity greater than 90%, such as seven peptides, six peptides or five peptides and other Oligopeptides, are obtained by enzymatic hydrolysis. This example is based on a molecular weight of less than 5,000 ears. 13 200916109 Like 'in particular, the smaller the molecule, the faster the body absorbs. Therefore, the molecular weight of the functional peptide is better distributed in the range of less than 3_boar' and the optimal range is between the occupational ^^(8) ear. The edible carrier can also include animal glue, sea turn, Chinese herbal extract, gelatin, starch, water or other phase-dispersing substances. Fifth Embodiment (Immune Modulator of Deep Sea Source Peptide) The fifth embodiment of the present invention discloses an immunomodulator for a deep-sea source peptide, the composition of the immunomodulator comprising the hydrolysis of the naked steroid from the enzyme. The functional peptide and the carrier capable of uniformly dispersing the functional peptide, wherein the enzyme hydrolysis step of obtaining the functional peptide and the functional peptide are derived from the naked enzyme hydrolysate, etc. As described in the first embodiment, the description is not repeated here. The functional peptide obtained by the hydrolysis of the enzyme has a molecular weight of less than 5 Torr, and the obtained functional peptide is extremely pure, and can be used as an immunomodulatory effective active ingredient for effectively promoting antibody secretion. Moreover, in order to enable the user to effectively absorb, the molecular weight distribution of the functional peptide is preferably less than 3,000 ears, and the optimum range is from 2 〇〇 to 1 〇〇〇. Comparative Example 3 Comparative Example 3 Comparison of the fourth and fifth embodiments of the present invention, comparing the degree of difference between the functional peptide obtained from the deep sea source and the control group in activating immune cells and promoting antibody secretion. As shown in Figures 4A and 4B, the present invention achieves functional peptides of naked scorpionfish by enzyme hydrolysis at different concentrations (〇.2mg/ml, 0.4mg/ml, 0.79mg/ml, 1.57). Mg/ml, 3.14mg/ml, 6.28mg/ml) were added to the culture medium of immune cells (hb4C5) to observe the effect of activating immune cell growth, and the secretion of IgM antibody from immune cells was compared with a control group. . Fig. 4A shows the growth effect of activated immune cell HB4C5, and Fig. 4B shows the amount of IgM secreted by the immune cell HB4C5 after activation of the functional peptide. Among them, when the growth effect of the activated immune cell HB4C5 was 100% in the control group, the effect of the growth of the immune cell HB4C5 by the naked steroid functional peptide obtained by the hydrolysis of the enzyme was up to 134%; while the control group activated the immune cell HB4C5 after the IgM When the secretion amount is 100%, the secretion amount of the naked scorpion-like peptide-activated immune cells obtained by the hydrolysis of the enzyme in 200916109 is up to 122%. It is apparent that the functional peptide of the deep-sea source of the present invention can effectively activate the immune cells and Promote antibody secretion and achieve immune regulation. Sixth Embodiment (Human Body Free Radical Scavenging Material of Deep Sea Source) The sixth embodiment of the present invention discloses a deep-sea source and free radical scavenging which can remove free radicals around cells, free radical damage and enhance the antioxidant capacity of the ship cells. Matter f, this human body free radical scavenging material can be used alone to make thieves and other edible noodles or general leather materials into a health care food, silk or antioxidant stabilizer. The towel, the human body free radical scavenging f system contains the function of obtaining a good amount of less than 5GGG lunar ear from the naked body through the enzyme water, earning the peptide, and the step of hydrolyzing and dislocating the towel enzyme is the same as that of the second embodiment. Directly hydrolyze the naked scallops obtained from the fishing, and complete the hydrolysis of the enzymes to obtain the enzyme water test of the f-containing machine, and then carry out the step of removing the sulphate hydrolysate. Although the functional peptide of Kewei can be used as an effective active ingredient for scavenging free radicals. This embodiment differs from the previous case in that the functional peptide is obtained from the naked mites, and the source thereof is selected from the group consisting of Benthosemaspp., Diaphusspp, and genus Lampcle ctusspp.), Cerat〇sc〇plusspp, torch lampfish (5) Ningru (10) Township.), Backlightfish (N〇t〇SC〇Pdusspp·), lampfish ( Symb〇1〇phmsspp), Stenobmchiusspp., Myct〇phumspp, and the group. After extraction, the functional peptides with a purity higher than 9G%, such as seven peptides, six-win or five-peptides, and other oligopeptides, are obtained by enzyme hydrolysis, and the molecular weight is less than 5〇〇 in this embodiment. The ear canal is the object of collection, especially the smaller the molecule, the body speed is touched, the molecular weight of the youngest progeny is better distributed in the range of less than 3000 ears, and the best range is between 2〇〇 and 1〇〇〇. ear. Comparative Example 4 Comparative Example 4 compares the degree of difference between the functional peptide obtained in the sixth embodiment of the present invention and the control group from the activity of 15 200916109. After the enzyme hydrolysis and depurination steps, the naked cockroach functional peptide was obtained, and its ability to scavenge 2,2-diphenyl-l-piCrylhydrazyl (DPPH) free radicals (DppH scavenging power) and chelate iron ability were analyzed. Since vitamin C is known to be a compound with strong antioxidant activity and effective scavenging of free radicals, and EDTA is an antioxidant compound known to have strong ion chelation, it is 2 〇〇 ppm of vitamin C and 20 分别, respectively. The EDTA of ppm was used as a positive control group (antioxidant efficacy 1〇0%), thereby comparing the degree of scavenging free radicals and antioxidation of the functional peptide. The results are shown in Table 1. When the DPPH scavenging power of vitamin C at 2〇〇ppm is 95.18±0.62°/〇, the DppH scavenging power of the naked-type deep-sea functional peptide obtained by the enzyme hydrolysis and de-purification step is 93.88±0.66% 'is equivalent to the DPPH scavenging power of the positive control group (97 °/〇 of the positive control group of vitamin C); while the EDTA chelated iron capacity of 200 ppm is 99.76±0.12%, it is hydrolyzed by enzyme hydrolysis. The chelated iron capacity of the naked-type deep-sea functional peptide obtained in the step was 85%±3 56%, which was also not far from the positive control group (84% of the positive control EDTA), and the deep sea source function of the present invention was apparent. Sex peptides are effective in scavenging free radicals and have good antioxidant capacity. A deep-sea source of functional peptides 舆 纰 浩 浩 浩 浩 自由基 能力 能力 能力 样品 样品 样品 IT IT IT IT IT IT IT IT IT IT IT IT 95 95 95 95 95 95 95 95 95 95 95 95 95 95 95 95 95 95 95 95 95 95 95 95 95 95 95 95 95 95 95 ±0.62 99.76±0.12 93.88±0.66 85.96±3.56 Seventh embodiment (lactic acid fermented material from deep sea) The seventh embodiment of the present month is a deep-sea source lactic acid fermentate obtained by enzymatic hydrolysis of naked meridians. New Mis's and Hunan-level bribery. The lactic acid has the antibacterial function, and can be used as a hybrid active ingredient of the muscle m system, or it can be used as a food phase or as a active ingredient of a natural health food together with a lactic acid bacteria. The step of obtaining the lactic acid fermented material derived from the peptide is as shown in the schematic diagram of the extraction process in Fig. 5, firstly, 16-1616109 is used to hydrolyze the naked sperm obtained by the fishing. After the hydrolysis is completed, an enzyme hydrolyzate rich in functional peptides is obtained, and the enzyme hydrolyzate is subjected to lactic acid fermentation to obtain a lactic acid fermentation product. Among them, the strain used for lactic acid fermentation is selected from the group consisting of Lactobacillus spp., Lactococcus spp., Pediococcus spp., and Streptococcus spp. Bifidobacterium spp. and its group. In addition, the functional peptide enzyme is hydrolyzed from the naked mites, and the source of the naked mites is selected from the group consisting of Benthosemaspp., Diaphusspp., Lampanyctusspp, and horns. Ceratoscoplusspp., Lampadenaspp., Notoscopelusspp., Symb〇i〇phrusspp, Sten〇brachius Spp. The genus Myctophumspp. and its group. Eighth Embodiment (Skin-derived skin external bacteriostatic agent) The eighth embodiment of the present invention is a skin external bacteriostatic agent containing a naked sputum-derived lactic acid fermented product, the dermatological external skin bacteriostatic agent The lactic acid fermented product obtained by hydrolysis of the naked I-type enzyme by the enzyme is obtained by lactic acid fermentation, and the lactic acid fermented product having the bacteriostatic effect, and the carrier capable of uniformly dispersing the lactic acid fermented product can effectively inhibit the Streptococcus mutans (Staphylococcus ediperalidis) and acne bacteria (Propicnibateriumacnes) and other body surface flora to achieve skin cleansing and bactericidal purposes. The lactic acid-fermenting substance derived from the naked genus is obtained as shown in the schematic diagram of the extraction process of the fifth drawing in the seventh embodiment, and the naked mites obtained by the fishing are first hydrolyzed by the enzyme. After the hydrolysis is completed, an enzyme hydrolyzate containing a functional peptide is obtained, and then the enzyme hydrolyzate is subjected to lactic acid fermentation, and finally a lactic acid fermented product having a bacteriostatic function is obtained. Among them, the strain used for lactic acid fermentation is selected from the group consisting of Lactobacillus spp., Lactococcus spp., Pediococcus spp., and Strept〇coccus Spp. .), and Bifidobacterium spp. and the group of the group, and the source of the functional peptide is obtained from the naked mites' source is selected from the group of Benth〇semaspp. Diaphusspp., Lampanyctus spp., Ceratosc〇plus spp, Tortoise, Xiningru (10) 17 200916109 SPP.), Backlight fish (Notoscopelusspp) .), Symbolophrus spp., Stenobrachius spp., Myctophumspp., and groups of groups. Further, the external antibacterial agent for skin made of the lactic acid fermented product having the antibacterial function obtained in the present embodiment can be further made into a skin external deodorant having a deodorizing function. Comparative Example 5 Comparative Example Five shows the lactic acid fermented product produced by the lactic acid fermentation of the functional peptide derived from the naked genus in the seventh embodiment, the eighth embodiment, and the ninth and tenth embodiments below. The bacteriostatic results of Listeria monocytogenes and Propicnibaterium acnes compared to the control group. Listeria is one of the common strains causing food contamination, while acne bacillus is the main species that induces acne on the skin. As shown in Table 2, the lactic acid fermentation product produced by the lactic acid fermentation of the functional peptide of the naked scorpion source has a bacteriostatic condition for Listeria, compared with the MRS basal medium of the control group, and the diameter of the inhibition zone is 〇9. The centimeters of the peninsula is 1.0 cm; for the bacteriostatic effect of the acne bacillus, compared with the basal medium of the control group, the I.7 cm of the inhibition zone is increased to 1.9 cm. It is obvious that the naked-faced machine is a lactic acid fermented product, which has significant antibacterial activity against Listeria and Acne bacillus, so it can be used as an active ingredient for skin external bacteriostatic agents and food antiseptic or health foods. Table 2: The bacteriostatic energy of the lactic acid fermented material and the control group of the bare-headed source is the inhibition circle (cm). The bacterium, the bacterium, the bacterium, the L9, the bare-line hydrolyzate λ/TOQ a 〇
第九實施例（深海來源之食品防腐劑） 本發明之帛九實施例係為-種符合食帛安全性*能廣泛應用於食品加工咬 食品保鮮中之食品P域劑，食品防細至少包含有由裸_經由酵ϋ解取^ 200916109 之機能性胜肽，經過乳酸發酵，取得乳酸發酵物，以有效抑制食品中所含有之 ^病菌，，如上述比較例五中所示，該乳酸發酵產物可以有效抑制食品污染中 常見之李斯㈤她麵咖咖），故可用於食品力江中，作為食品防 腐劑之有效活性成份。 上述食品防腐劑之有效活性成分之取得步驟，如同前述第七實施例中所述， 在此不再重複贅述。其中’進行乳酸發酵所仙之菌種，係選自乳酸桿菌屬 (Lactobacillus spp.)^L«i^ (Lactoc〇ccus spp〇 (Pedi〇coccus spp.)^iiM ( Streptococcus spp.)^^#g^ ( Bifidobacterium spp.) A 所組之組群，而該機能性胜肽之來源係萃取自裸經類，來源係選自底燈魚属 (Benthosemaspp.)、眶燈魚屬（Diaphusspp.)、珍燈魚屬（Lampanyctusspp )、 角燈魚屬（Ceratoscoplusspp.)、炬燈魚屬（Lampadenaspp )、背燈魚屬 (N〇t〇SC〇Pelusspp.)、標燈魚屬（Symbol〇phrusspp )、臂燈魚屬（Sten〇brachius SPP.)、燈籠魚屬（Myctophumspp.)及所組之組群。 第十實施例（維持消化道正常機能之保健食品） 本發明之第十實施例係為一種符合食用安全性且能整腸健胃之保健食品，此 保健食品之有效成份，係來自裸經類經由酵素水解後取得之機能性胜肽，再經 由乳酸發酵所取得之乳酸發酵物，將該乳酸發酵物添加至可均質分散該乳酸發 酵物之食用性載劑後，即製成一保健食品。 其中’此保健食品之有效活性成份，如同前述第七實施例中所述，在此不再 重複贅述，惟進行乳酸發酵所使用之菌種，係選自乳酸桿菌屬（Lactobacillus SPP.)、乳酸球菌属（Lactococcus spp.)、足球菌屬（Pediococcus spp.)、鍵球菌屬 (Streptococcus spp.)，及雙歧桿菌屬（Bifidobacterium spp.)及所組之組群，而 該機能性胜肽之來源係自裸鰛類酵素水解物，尤其裸鰛類係選自底燈魚屬 (Benthosemaspp.)、眶燈魚屬（Diaphusspp.)、珍燈魚屬（Lampanyctus spp.)、 角燈魚屬（Ceratoscoplus spp.)、炬燈魚屬（Lampadena spp.)、背燈魚屬 19 200916109 (Notosc叩elusspp.)、標燈魚屬（Symb〇1〇phrusspp )、臂燈魚屬⑽如咖咖耶 spp.)、燈籠魚屬（Myctophumspp.)及所組之組群。裸鰛類來源之乳酸發酵物 中含有多量的乳酸菌，而且總乳酸菌數的含量符合保健食品要求含量每毫升1〇8 CFU以上’因此也可以作為平衡腸道有益n、轉消化道正常機能之有效活性 . 成份。 睛參酌以下表五所示，將裸鰛類乳酸發酵物之水溶液施以適當的噴霧乾燥處 理，其中仍富含乳酸菌，亦可製成健胃整腸的保健食品。 比較例六 比較例六係顯示本發明之第九及第十實施例中，裸鰛來源之機能性胜肽經由 乳酸發酵後產出的乳酸發酵物具有食品安全性、抗致突變性及所含之乳酸菌數 疋否尚於保健食品要求之乳酸菌數標準。 - 表三裸經類來源乳酸發酵物與對照組經由Ames test比較Ninth Embodiment (Food Preservative of Deep Sea Source) The ninth embodiment of the present invention is a food P domain agent which can be widely used in food processing bite food preservation, and the food antifouling includes at least The lactic acid fermentation product is obtained by lactic acid fermentation to obtain a lactic acid fermentation product by lactic acid fermentation, thereby effectively inhibiting the pathogenic bacteria contained in the food, as shown in the above Comparative Example 5, the lactic acid fermentation. The product can effectively inhibit the common Liss (five) her face coffee and coffee in food pollution, so it can be used in food Lijiang, as an effective active ingredient of food preservatives. The steps of obtaining the effective active ingredient of the above food preservative are as described in the foregoing seventh embodiment, and the detailed description thereof will not be repeated here. Among them, 'the bacterium of lactic acid fermentation is selected from Lactobacillus spp. ^L«i^ (Lactoc〇ccus spp〇(Pedi〇coccus spp.)^iiM (Streptococcus spp.)^^# G^ (Bifidobacterium spp.) A group of the group, and the source of the functional peptide is extracted from the naked genus, the source is selected from the group consisting of Benthosemaspp., Diaphusspp. Lampanyctusspp, Ceratoscoplusspp., Lampadenaspp, N〇t〇SC〇Pelusspp., Symbol〇phrusspp, The genus Sten〇brachius SPP., Myctophumspp., and the group of the group. The tenth embodiment (health food for maintaining normal functions of the digestive tract) is a tenth embodiment of the present invention. A health food that meets the safety of food and can be used for the whole stomach and stomach. The active ingredient of the health food is a lactic acid fermented product obtained by lactic acid fermentation from a functional peptide obtained by hydrolysis of an enzyme through an enzyme, and the lactic acid is obtained by lactic acid fermentation. The fermented product is added to the food which can homogenize and disperse the lactic acid fermentate After the carrier, the health food is prepared. The effective active ingredient of the health food is as described in the seventh embodiment, and the details are not repeated here, but the strains used in the lactic acid fermentation are It is selected from the group consisting of Lactobacillus SPP., Lactococcus spp., Pediococcus spp., Streptococcus spp., and Bifidobacterium spp. a group of the group, and the source of the functional peptide is derived from a naked enzyme hydrolysate, especially the naked genus is selected from the group consisting of Benthosemaspp., Diaphus sp., and the lampfish. Genus (Lampanyctus spp.), Ceratoscoplus spp., Lampadena spp., genus 19 200916109 (Notosc叩elusspp.), genus of genus (Symb〇1〇phrusspp) ), the genus of the genus (10) such as café (spp.), the genus of the genus (Myctophumspp.) and the group. The lactic acid fermentation source from the naked cockroach source contains a large amount of lactic acid bacteria, and the total lactic acid bacteria content is in accordance with the content of health food requirements of more than 1 C 8 CFU per ml. Therefore, it can also be used as a balanced intestinal tract. Active. Ingredients. According to the following Table 5, the aqueous solution of the naked lactic acid fermented product is subjected to appropriate spray drying treatment, which is still rich in lactic acid bacteria, and can also be used as a health food for stomach and intestines. Comparative Example 6 Comparative Example Six shows that in the ninth and tenth embodiments of the present invention, the lactic acid fermented product produced by the naked cockroach-derived functional peptide via lactic acid fermentation has food safety, anti-mutagenicity and inclusion. The number of lactic acid bacteria is still in the number of lactic acid bacteria required for health food. - Table 3: Raw lactic acid fermented materials from the bare-headed source were compared with the control group via Ames test
Sal. Typhimurium TA98 Sal. Typhimurium ΤΑ 100 _____平均菌數(CFU/盤)_ 對照組 50.5±13.44 ( 100% ) 69.5士6.36 ( 100% ) 裸鰛類來源 乳酸發酵物 62.5士0.71 (124%) 73.0±1.41 (105%) 如表二所不’將裸鰛類水解後進行乳酸發酵所取得之乳酸發酵物，利用Ames test測5式其食用安全性，觀察&/·如/zz'mwWwm TA98及似 Typhimurium ΤΑ 100 兩種函株之平均菌數(No. of surviving colonies/plate) 。結果顯示，裸經 類來源之乳|文發酵物之組中^.沙尸知細咖所TA98及5^/. 2^p/»>m#7.wmTA100 兩種菌株之平均菌數(No. of surviving colonies/plate)分別為 62.5±0.71 CFU/盤及73·〇±ι.41 CF盤，而同時對照組的平均菌數則為50 5±13 44 CFU/盤及69.5±6,36 CFU/盤，分別是對照組的124%及105%，顯見該裸鰛 20 200916109 類來源之乳酸發酵物不具有食品毒性。 此外如表四所示’測試不同濃度（〇〇5M、〇 、〇遷）之裸經類來源酵 素水解物及紐發酵物之抗致突醉。結細*，、GUI的裸經 類來源之酵素水解液的抗致突變率分別為m w、％观及％爆娜農度的 裸類來源之乳酸發酵物之抗致突醉則分別為⑻观、及处1%。顯 見該二物質皆具有抗致突變性 四裸經類來遁乳酸發酵物之抗致突變率 添加量 Sal. Typhimurium 抗致突變率 (毫升)Sal. Typhimurium TA98 Sal. Typhimurium ΤΑ 100 _____ Average number of bacteria (CFU/disc) _ Control group 50.5±13.44 (100%) 69.5 ± 6.36 (100%) Lactate-derived lactic acid fermentate 62.5 ± 0.71 (124%) 73.0±1.41 (105%) As shown in Table 2, the lactic acid fermented product obtained by lactic acid fermentation after hydrolysis of naked mites is tested by Ames test for food safety. Observe &/· such as /zz'mwWwm The average number of bacteria (No. of surviving colonies/plate) of TA98 and Typhimurium ΤΑ 100. The results showed that the average number of bacteria of the two strains (No.1, the number of bacteria in the group of the fermented materials of the genus The surviving colonies/plate were 62.5 ± 0.71 CFU/pan and 73·〇±ι.41 CF plates, respectively, while the average number of bacteria in the control group was 50 5 ± 13 44 CFU/pan and 69.5 ± 6,36 The CFU/disc was 124% and 105% of the control group, respectively, and it was found that the lactic acid fermented product of the 200916109 source was not food-toxic. In addition, as shown in Table 4, the test was carried out to test the different concentrations (〇〇5M, 〇, 〇) of the naked source-derived enzyme hydrolysate and the fermentation of the new fermentation. The anti-mutagenic rate of the enzyme hydrolysate from the bare-type source of the GUI is mw, % view, and the anti-inducement of the lactic acid fermented product of the naked source of the genus of the genus, respectively, is (8) 1%. It is apparent that the two substances are all resistant to mutagenicity. The anti-mutagenic rate of the fermented lactic acid fermented by the four naked cells is added. Sal. Typhimurium anti-mutagenic rate (ml)
0.2 50 發酵物 0.1 59 0.05 42 93.1 84.2 101.0 而口參閱表五，不論是裸_來源之乳紐_或是進—步經财霧乾燥處理 所製得之裸乳酸發酵喷乾粉，其中總乳酸分別為每毫升ΐ 4χ⑺ 及3.4 X 1〇1〇CFU，均高於中關家標準（CNS，為每毫升含菌落數1〇7)。 表五一裸魅類來源乳酸發酵物及乳酸發酵噴乾粉之總乳酸菌含詈 裸鰛類乳酸發酵液 總乳酸菌數(CFU) 1.4 X 1011 裸鰛類乳酸發酵喷乾粉_3.4 X 1〇10___ 乳酸發 綜上所述’本發明之裸鰛類來源之機能性胜肽經由乳酸發酵後產出的 21 200916109 酵物，經Ames test亦證實裸鰛類乳酸發酵物無毒性、具有抗致突變性，且乳酸 菌數滿足健食品的乳酸紐含量鮮，此乳酸發酵物不僅天然，用於食品安 全無虞，而且乳酸發酵物中飽含豐f的乳_，也可幫助人體達馳胃=的 功效，並作為保健食品維護腸胃道機能之天然有效活性成分。 以上所述僅為本發明之較佳實施例，並非用以限定本發明之權利範圍；同時 以上的描述，騎熟知本技術钱之專門人士應可日膽及實施，目此其他未脫 離本發睛揭故㈣下所絲的雜改魏簡，均聽含在下述之申請專 利範圍中。 【圖式簡單說明】 第1圖係為本發明第二實施例之流程示意圖； 第2A圖係為本發明之深海來源之機能性胜肽與對照組之活化皮膚之纖維母 細胞比較結果圖； 第2B圖係為本發明之深海來源之機能性胜肽與對照組之活化皮膚之角質細 胞比較結果圖； 第3A圖係為本發明之深海來源之機能性胜肽與對照組之促進膠原蛋白合成 作用之比較結果圖； 第3B圖係為本發明之深海來源之機能性胜肽與對照組之皮膚彈性度之比較 結果圖； 第3C圖係為本發明之深海來源之機能性胜肽與對照組之皮膚水分散失改善 度之比較結果圖； 第3D圖係為本發明之深海來源之機能性胜肽與對照組之皮膚水分含量增加 程度之比較結果圖； 第3E圖係為本發明之深海來源之機能性胜肽與對照組之皮膚緊實度之比較 22 200916109 結果圖， 第4A圖係為本發明之深海來源之機能性胜肽與對照組之活化HB4C5免疫 細胞比較結果圖； 第4B圖係為本發明之深海來源之機能性胜肽與對照組之活化Hgicf免疫 細胞之IgM分泌量比較結果圖； 第5圖係為本發明第七實施例之裸經類來源乳酸發酵物之取得流程圖。 【主要元件符號說明】 無 230.2 50 Fermentation 0.1 59 0.05 42 93.1 84.2 101.0 Refer to Table 5 for the mouth, whether it is bare _ source of milk _ or _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ For each milliliter of χ 4χ(7) and 3.4 X 1〇1〇CFU, both are higher than the Zhongguan standard (CNS, the number of colonies per ml is 1〇7). Table 5: Naked charm source lactic acid fermentation product and lactic acid fermentation dry powder total lactic acid bacteria containing lanthanum lactic acid fermentation broth total lactic acid bacteria number (CFU) 1.4 X 1011 naked lactic acid fermentation dry powder _3.4 X 1〇10___ lactic acid In summary, the 21 200916109 protein produced by the lactic acid fermentation of the functional peptide derived from the naked mites of the present invention, the Ames test also confirmed that the naked lactic acid fermented product is non-toxic, has anti-mutagenicity, and the lactic acid bacteria The number of lactic acid products that meet the health food is fresh, and the lactic acid fermented product is not only natural, but also safe for food, and the lactic acid fermentation product is rich in milk, which can also help the body to achieve the efficacy of the stomach and as a health care. Foods maintain natural and effective active ingredients of gastrointestinal function. The above description is only the preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. At the same time, the above description, the special person who is familiar with the technical money should be able to implement it, and the other is not divorced from the present invention. Wei Jian, who is under the guise of (4), is included in the scope of the patent application below. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic flow chart of a second embodiment of the present invention; FIG. 2A is a comparison result of a fibroblast of a deep-sea source functional peptide of the present invention and a activated skin fibroblast of a control group; 2B is a comparison result of the keratinocytes of the activated skin of the deep sea source of the present invention and the activated skin of the control group; FIG. 3A is a collagen promoting protein of the deep sea source and the control group of the present invention. The comparison result of the synthesis is shown in Fig. 3B is a comparison result of the skin elasticity of the deep-sea source functional peptide of the present invention and the control group; the 3C figure is the functional peptide of the deep sea source of the present invention and The comparison result of the skin water dispersion loss improvement degree of the control group; the 3D figure is a comparison result of the skin moisture content increase degree of the deep sea source functional peptide of the invention and the control group; FIG. 3E is the invention Comparison of skin firmness between functional peptides of deep-sea source and control group 22 200916109 Results map, Figure 4A is the activation of the functional peptides of the deep sea source of the present invention and the control group Figure 4B is a comparison result of the IgM secretion amount of the activated Hgicf immune cells of the deep-sea source functional peptide of the present invention and the control group; FIG. 5 is a seventh embodiment of the present invention. A flow chart for obtaining a lactic acid fermented product from a naked cell. [Main component symbol description] None 23