TW200839211A - Flow cell and a particle shape abalyzing device having the flow cell - Google Patents
Flow cell and a particle shape abalyzing device having the flow cell Download PDFInfo
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1404—Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
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200839211 ^ 九、發明說明： -【發明所屬之技術領域】 - 本發明係有關用以取得懸浮於液體中之私工一 ,訊之流體觀測單元及具備流體子之形態貧 置。 平7°之粒子形狀分析裝 【先前技術】 在/亏水、農業用水、工孝用 掌、$/ 系用水荨之竽水廠、和化學工 :广石油化學工業、生物產業、食品工孝等之：箱 工廄，為了進行品質管理等， 菜寺之各種 粒子之形狀測定。特別是:在高:c使用之 fJ拌古挑处… 饿月b衣口口之製造廠，A 了 衣以阿機能製品，而進 、故4 了 鮭^ 仃旯倣小之粒子之形狀解析。 解析此種粒子形狀之技術係， 乾式分散或濕式分散使粒子分散後之以直採用將經 而進行形狀解析之方丰 ’、以頭微鏡等拍攝 粒子散布於椤太卜尨从孩 欢之方去，係指將 、1处 由於粒子間之凝聚、附著、互相*田 法使粒子確實地分散 互相重$，故無 狀解析之問題。 套進仃早-粒子之正確地形 八$另—方面，所謂經濕式分散之方法，係指 :政於分散媒中後，拍攝該狀態之粒子而：粒:(分散^ 然而’在此方法中，由於粒子合 订/狀解析 媒中，故即使以㈣#古姑 次元地分散於分散 大半之教子上之二 攝此狀態，仍有無法聚焦* 針對此問題’有製作以載體溶液將含有粒子之樣品液 320011 5 200839211 包入，成之樣品流，並將該樣品流通過流體觀測單元中而 令其呈爲平狀地流動，藉此使粒子通過顯微鏡等之觀 =焦點上之方法。而且，已有使用此方法，拍攝含於：品 流中之粒子，並將其粒子之影像及形狀解析，藉此自動营 出粒子之大小和形狀等之粒子影像分析： 如：參照專利文獻1及2)。 圾木C例 此種粒子影像分析褒置中所使用之流體觀測單元 (flow cell)係由形成為使從兩側將樣品流所流經之流路 之-方向之寬度緊縮而成之緊縮部、與接續於該緊縮部之 下游侧且形成直線狀之流路之平行部所構成，於該平行部 係設置有使用於拍攝(觀測)粒子之光穿透之 如：參照專利文獻3)。 1 〔專利文獻1〕日本特開平9_126988號公報 〔專利文獻2〕日本特開2〇〇6_84243號公報 〔專利文獻3〕日本實公平6-18275號公報 【發明内容】 (發明欲解決的課題） 然而，在如上述之流體觀測單元中，自於透光部設置 於平灯部内’故上述之粒子影像分析裝置會拍攝流經直線 狀之流路内之樣品流。而且，此種流經直線狀之流路内之 板〇口 /瓜係王彳之平行部表面之距離相同之面内流速一定之剪 流（shear flow)。例如：當含於樣品流之中心部之2個粒 子在互相重豐之狀態下流人直線狀之流路中後，此2個粒 子會被粒子影像分㈣㈣測成丨個粒子，而有有時會無 320011 6 200839211 法正確地偵測出粒子之形狀等之問題。 -由於由此種剪流所形成:剪流場會產， -、;有使_内之粒子旋轉或攪拌之作用。因此，若樣品 S’L流經直線狀之流路内，則合於 、 rn ^ ^ ^ ； Μ水品流中之粒子會被旋 =授放，而使粒子移動到非顯微鏡之焦點位置之 在特定姿勢之粒子之形態資訊之問^法正確地件到保持 , 並且’在直線狀之流路中，因牆 =;央部最快，越接近牆面(越接近流體觀測 卜，）則變的越慢。其結果，含於樣品流中之粒子之^ f在平行部之寬度方向，各粒子之移動速度會依粒子相對 於樣品流之中央部之相對位 3依拉子相對 離戸1、、☆ Φ 士 A , #位置而不同。因此’即使為例如·· 方方向（上游側往下游側之方向），且平行部之寬产 。相對於樣品流之中央部之相對位置不 相重疊之狀態）之粒子流直$ 心（未互 狀之流路内之樣二之流路中後’也因在直線 樣一之流速分布之影響，在透光邻m 個粒子互相重A 你返尤4形成2 ^ ^ 且狀怨，而粒子影像分析裝置有有時奋盔 1確地_出粒子之形狀等之問題。換言之，在“二 ηΓ 表面側之狀態下’先流入直線狀 較粒子ρι遲播、、…、在位於木一之中央部之狀態下， , 机入直線狀之流路中之粒子P2，2個#早奋 在透光部互相重疊，而粒子^與粒子會 析裝置偵測成1 h， 曰破粒子影像分 、、成1個粒子，結果有有時會盔法得ξ丨丨n r 子之形態資訊之問題。 …亍到正確的粒 320011 7 200839211 :::明係有鑑於上述原因，而以提供能生成具有高流 性’亚且複數個粒子呈互相重疊之狀態之機率小， 到維持於特定姿態之狀態之粒子之形態之資訊之费 ,口口机之流體觀測單元及具 ^ 析裝置為目的。 之粒子形狀分 (解決課題的手段） 你、Γ轉決上述課題之本發明之第1態樣係，具有形成 使以載體溶液將含有粒子 叙厂“ ㈣子之樣扣液包入而成之樣品流所流 路且形成為將該流路緊縮而成之緊縮部、與使光線 、過之透光部之流體觀測單 =、、’ 形成為使前述流路之截面：二=“緊縮部係 内之樣品流穩定地呈平面少，且流經前述流路 設置於前述緊縮部内之流體觀、且料透光部係 :=延二流動，係指在流路内之同一處(在從流路上游 内之樣品流之汽速為^ 部表面之距離相同之面 從緊r都疋’且樣品流之流出方向之速度依 流動、、：之上㈣之距離而以-定比率增加之穩定地延伸 在此種第丨態樣中，由於能生成穩定地 -動之樣品流，故在流出方向產生一定 ，伸 力）成為弭士二疋之應力（千面伸張應 外，穩定地^ 能使樣品流之流動安枝提高。此 在流出方面狀延伸流動係在樣品流流向流路内時， 向上會經常持續地被拉長，其 -路内之複數個粒子呈互相重4之狀態之機二= 320011 8 200839211 =古，外由於對於含於樣品流中之粒子經常施加垂直流 ’二之力，故不會使粒子旋轉及擴散，而能使粒 C路内最不會受到流體阻力之姿態而移動通過 ，：丄亚t，由於連續地使流路之截面積減少，故能使 二乂1之‘品流之厚度變的極薄，並且能輕易且精密地 &制在▲體觀測單元内之樣品流之位置及厚度。 本發明之第2態樣係，前述緊縮部形成為使前述流路 '之剖面呈矩形之第1態樣之流體觀測單元。 此種第2恶樣係能輕易地製作流體觀測單元。 =明之第3態樣係將前述緊縮部形成為使從兩側將 …路之—方向之寬度緊縮，且形成為使從前述一方向 ^述流路之中央之距離與從前述緊縮部之上游端之距離 王反比地減少之第2態樣之流體觀測單元。 此種弟3態樣係能更輕易地製作流體觀測單元。 a本發明之第4態樣係，前述緊縮部形成成使從單側將 1流路之—方向之寬度緊縮，且形成為使從前述一方向 二！之一方向之寬度與從前述緊縮部之上游端之距 王反比地減少之第2態樣之流體觀測單元。 此種$ 4悲樣係與第3態樣同樣地，能更輕易 流體觀測單元。 衣作 之料月之第5怨樣係’前述透光部設置於前述緊縮部 觀測::端部上之第1態樣至第4態樣中任-態樣之流體 上述之第5態樣係，由於越接近緊縮部之下游側，則 320011 9 200839211 上側之緊縮部與下側之緊縮 ‘位於透光部之上游側與 ：：；：的越小’故 _變小，而且也能 > 之肌路之上τ方向之寬度差 之也π p 厚度變薄。其結果，可使全邻 電荷二置; 本發明：'Πί;4結果’能更正4地取得粒子影像。 月之乐6恕樣係所有構件皆 第1態樣至”態樣中任—態所構成之 '之樣輕易地觀測流經流體觀測單元内 且備本:::之第7態樣係一種粒子形狀分析裝置，此裝置 態樣中任-態樣之流體觀測單元、二、f 2恶樣至“ 透她、光而偵測含於前述樣 ：= 之解析部。 丨《輸出而开出粒子之形態資訊 ^述第7態樣係能在短時間内取得大量正確的粒子與 態資訊。 川内且^"精度地測定該等粒子影像之形 (發明的效果） 數個安定性，且複 定姿態之狀態之粒子得到維持於特 及且借贫、六雕# y 人〇貝汛之祆口口、极之流體觀測單元 /、備該/瓜肢硯測單元之粒子形狀分析裝置。 卜田3於I品流中之粒子為如纖維狀粒子之細長 320011 10 200839211 形之粒子時，由於a丄 ，中，也合因上、f即使在例如扭曲狀態等下含於樣品流 ，粒子…穩定地呈平面狀延伸流動而在流路内之 —二朝流二]::與 ^ ，、、、、口果此防止該粒子之凝聚而使 =果。° 方向，而發揮能更正確地取得形態資訊之 光邙：二！地使流路之截面積減少’故能使在透 之厚度變的極薄，並且能輕易且精密地控制 在、•肢硯測單元内之樣品流之位置及厚度。 【實施方式】 & =下，說明關於本發明之實施方式。再者，本實施形 您之5兒明僅為例示，本發明得為 、 (實施形態Π 才不又叮之說明所限定。 第y係表示本發明之實施形gl之粒子形狀分㈣ 4了：Ϊ:如第1圖所示，本實施形態之粒子形狀分析 v =係由为散機組（dlspersionunit)10、連接於分散機 、，且10之泵機組（卿punit)20、連接於栗機組2〇之觀 ，（obs⑽tlGnunlt)3G、連接於觀察機組3G之做為解析 狀解析機組（analyzing unlt)40、與連接於該等所有 組上之控制機組（⑶ntrol unit)50所構成。再者，本者於 :態係如後述，觀察機組3。係由樣品流製作部、流體二： 早元（How cell)及粒子偵測部所構成。 分散機組1〇係具有使做為分析對象之粒子分散 品液中後將樣品液供給至泵機组2()之機能者。可舉例^ 320011 11 200839211 、ζ裝有具有強力攪拌機能之攪拌器之漏斗狀之供給裝置 泵機組㈣、具有將供給自分散機組1()之樣品液 •先儲藏之載體溶液供給至觀察機組3G之機能，並且具有能 正確地控制各液體之供給量之機能者。 古、、觀察機組30係如第2圖所示，由樣品流製作部Η、 Γ述=後述之设置有使光線透過之透光部322之流體觀測 早：、32、與配置成將流體觀測單元&之透光部322夹住 之妝光部33及粒子偵測部34組成。 口 σ 、樣品流製作部31係具有能使用供給自泵機組20之樣 口口 =與載體溶液’製作以載體溶液將樣品液包人而成之樣 流後，供給至流體觀測單元之機能者。 ^ /樣品流製作部31係可舉例如：由載體所流經之剖 矩形之管狀構件、與插入於該管狀構件之上游側中央部中 並將樣品液吐出之樣品液喷嘴組成，且能將樣品液注入产 經官狀構件之載體溶液之中央部者等。 爪 光P 33係由閃光燈等光源331與旋轉濾波器332 :由二Ϊ有高速快門機能。另一方面，粒子偵測部34 D攝影機341與透鏡342組成，且能經由接 源331昭身十φ并办、采i。+ 饮队曰尤 ， =射出亚牙透樣品流而來之光，而取得粒子影像， 該等資訊輸出至外部。因此，觀察機组30係能藉由照 :二與粒子偵測部34 ’在短時間内且連續地取得鮮明 、里粒子影像，並將該等輸出至外部。 解析機組40係具有解析輪出自觀察機組30之粒子影 320011 12 200839211 t之含於樣品流中之粒子之直徑和最大長等形離資 般個人^^析機組4G係可舉例如：具㈣種機能之一 奴個人电腦和專用計算機。 各機ΓΓΓ50係具有將控制信號傳送至各機組而控制 控制機組5。係可舉例如:具備該種J 瓜们人电腦和專用計算機，也可 成控制機組50與解析機組4()。 电月自構 \其次，詳細說明關於流體觀測單元32。第3圖係 ::形：之流體觀測單元之概略透視圖，第4圖係本實施： 體觀測單元之縱向概略透視圖。 赤入展弟目及第4圖所示’流體觀測單元32係由由玻璃 屬寻所構成之長方體形構件組成，且於左側側面上設 置有矩形之上游側開口 326’並且於右侧側面上設置有在 亡下方向扁平矩形之下游側開口 327。此外，於流體觀測 :兀32之内部係設置有與流路325之中央呈對稱形成之緊 縮P(throttle)321，使在連結上游側開口 326與下游側開 J 327之流路325全體從上下方向緊縮。而且，於此緊縮 邛321之下游側係設置有由透明之丙烯酸樹脂或玻璃等透 明構件所構成之透光部322。換言之，在透光部322，流路 卜也僅二許從上下方向受緊縮。因此，因如此構成流體 ^測單元32’而能一面得到由後述之緊縮部321產生之效 不，一面拍攝含於樣品流中之粒子之粒子影像。再者，此 種流體觀測單元32係能經由將緊縮部321所形成之2個構 件配置成形成有緊縮部321之面互相面對面，並且將形成 320011 13 200839211 於2個構件之間之流路之兩側面 叫田Μ板狀構件等塞住而製 ，ds ° • 在此，此緊縮部321係形成成使從流路325之中央 4圖中以L-L·線表示）之距離y盥從腎 . y /、攸緊細部之上游端之距離 X呈反比地減少，具體而言係形成成使滿足如下述之式卜 再者，關於下方之緊縮部32ϊ也相同。 y (ax + bj 式 i式緊：：及b係刀別為a> °、b> 0之任何常數，x係表示 從緊縮部之上游端之距離） β因如此形成緊縮部32卜而能使樣品流之流動安定性 ，：二且在樣品流流經流路325時，由於樣品流係朝流 上游側開〇 326朝下游側開^ 327之方向）以經 ^疋之加速度被拉長，故會發揮使複數粒子呈互相重Α 之狀態之機率降低之效果。換言之，例如：如»5圖（Γ) ώ由於即使粒子pl肖p2在互相重疊之狀態下存在於 取品流中，在樣品流流經流路325内時，樣品流係在上下 方向成型成扁平狀並且朝向流出方向，且以經常一定之加 ^度持續被拉長，故如第5圖⑻所示，含於樣品流中之粒200839211 ^ IX. Description of the invention: - [Technical field to which the invention pertains] - The present invention relates to a fluid observation unit for obtaining a private worker suspended in a liquid and having a form of a fluid sub-poor. Flat 7° particle shape analysis [Previous technology] In/or water loss, agricultural water, Gongxiao palm, $/ Department of water, water plant, and chemical industry: Guangzhou Petrochemical Industry, Bio-industry, Food Industry Etc.: Box workers, in order to carry out quality management, etc., the shape of various particles of the vegetable temple. In particular: in the high: c use fJ mix ancient picks ... Hungry month b clothing mouth of the factory, A clothing with the A functional products, and enter, therefore 4 鲑 ^ 仃旯 imitation of small particles shape analysis . The technique for analyzing the shape of such particles, dry dispersion or wet dispersion, after dispersing the particles, directly using the shape analysis of Fang Feng', and the particles of the head micromirror are scattered in the 椤太卜尨 from the child The reason for this is that there is a problem that there is no parsing in one place due to the aggregation, adhesion, and mutual field between the particles. In the early stage of the particle--the correct topography of the particle, the method of wet-dispersing means that after the government disperses the medium, the particles of the state are photographed: grain: (dispersion ^ but 'in this method In the case of the particle binding/analysis medium, even if the (four)# Gugu subdivision is dispersed in the state of the scattered half of the teachings, there is still no focus*. For this problem, the carrier solution will be produced. The sample liquid of the particles 320011 5 200839211 is packed into a sample stream, and the sample stream is passed through the fluid observation unit to flow in a flat shape, thereby passing the particles through a microscope or the like. Moreover, this method has been used to capture the particles contained in the product stream, and to analyze the image and shape of the particles, thereby automatically performing particle image analysis of the size and shape of the particles: For example, refer to Patent Document 1 And 2). The fluid flow cell used in the particle image analysis device is a compact portion formed by tightening the width of the flow path through which the sample flow flows from both sides. A parallel portion that forms a linear flow path on the downstream side of the constricted portion is provided, and the parallel portion is provided with light for imaging (observing) particles. See Patent Document 3). [Patent Document 1] Japanese Laid-Open Patent Publication No. Hei. No. Hei. No. 6-84243 (Patent Document 3). However, in the fluid observation unit as described above, since the light transmitting portion is provided in the flat lamp portion, the particle image analyzing device described above captures a sample stream flowing through the linear flow path. Further, such a flow is caused by a shear flow having a constant flow velocity in the plane of the parallel surface of the slab mouth/melon scorpion in the straight flow path. For example, when two particles contained in the center of the sample stream flow in a straight flow path in a state of mutual accumulation, the two particles are measured by the particle image (4) (4), and sometimes There will be no 320011 6 200839211 method to correctly detect the shape of the particles and so on. - Due to the formation of such a shear flow: the shear flow field will be produced, -,; it has the effect of rotating or stirring the particles in the _. Therefore, if the sample S'L flows through the linear flow path, it is combined with rn ^ ^ ^ ; the particles in the hydrophobic product flow are rotated and released, and the particles are moved to the non-microscope focus position. The information about the shape of the particles in a specific posture is correct to the piece, and 'in the straight flow path, because the wall =; the central part is the fastest, the closer to the wall (the closer to the fluid observation, then) The slower it becomes. As a result, the particles contained in the sample stream are in the width direction of the parallel portion, and the moving speed of each particle is relatively dependent on the relative position 3 of the particle relative to the central portion of the sample stream. Shi A, # location is different. Therefore, even in the direction of the square direction (the direction from the upstream side to the downstream side), the parallel portion is widened. The flow of the particles relative to the state in which the relative positions of the central portions of the sample flow do not overlap) is directly affected by the distribution of the flow velocity in the flow path of the sample. In the light-transmitting m particles, each other is heavy A. You return to 4 to form 2^^ and grievances, and the particle image analysis device sometimes has problems such as the shape of the particles. In other words, in the second In the state of the surface side of ηΓ, the first flow in the straight line is slower than the particle ρι, ..., in the state of the central part of the wood, the particles P2 in the straight flow path, 2 #早奋The light-transmitting portions overlap each other, and the particles and the particle-resolving device detect it for 1 h, smashing the particle image into and dividing into one particle, and as a result, there is a problem that the shape information of the nr is sometimes obtained by the helmet method. ...to the correct grain 320011 7 200839211::: In view of the above reasons, the Ming system has the advantage of being able to generate a state with high fluidity and a plurality of particles overlapping each other, to maintain a specific posture. The information fee of the state of the particle of the state, the fluid observation of the mouth machine For the purpose of the device and the device, the particle shape is divided (the means for solving the problem). The first aspect of the present invention, which has been formed by the above-mentioned problem, has a structure in which the carrier solution contains particles ("four") The sample flow through which the liquid is entrained is formed into a constricted portion formed by contracting the flow path, and a fluid observation sheet of the light passing portion and the light transmitting portion is formed, so that the flow is formed Cross section of the road: 2 = "The sample flow in the compacting system is stably flat, and the fluid flowing through the aforementioned flow path is disposed in the compacting portion, and the light transmitting portion is: The same point in the flow path (the velocity of the sample flow from the upstream of the flow path is the same as the distance from the surface of the surface, and the velocity of the flow out of the sample flow depends on the flow, and: (4) The distance is stably extended by a constant ratio in such a second aspect. Since a stable-moving sample flow can be generated, a certain amount of extension force is generated in the outflow direction to become a gentleman's stress. Thousands of face extensions, stable ^ can make the flow of sample flow When the flow of the sample flows into the flow path, the upward flow is often continuously elongated, and the plurality of particles in the path are in a state of being heavy by 4 = 320011 8 200839211 = In ancient times, because the particles contained in the sample stream often exert a vertical flow of 'two forces, the particles will not be rotated and diffused, and the particles C will be moved by the attitude of fluid resistance.丄亚t, because the cross-sectional area of the flow path is continuously reduced, the thickness of the product stream of the 乂1 can be extremely thin, and the sample flow in the ▲ body observation unit can be easily and accurately Position and Thickness According to a second aspect of the present invention, the tightening portion is formed as a fluid observation unit in which the cross section of the flow path is rectangular in a first aspect. This second type of sample system can easily produce a fluid observation unit. The third aspect of the invention forms the tightening portion so as to narrow the width of the path from both sides, and is formed such that the distance from the center of the flow path from the aforementioned direction is upstream from the tightening portion The second aspect of the fluid observation unit is reduced in inverse proportion to the king. This kind of 3 state system makes it easier to make a fluid observation unit. According to a fourth aspect of the present invention, the tightening portion is formed such that the width of the one-way path is contracted from one side, and is formed so as to be from the one direction. A fluid observation unit of a second aspect in which the width of one direction is inversely proportional to the distance from the upstream end of the aforementioned constricted portion. This $4 sadness is the same as the third aspect, making it easier to observe the fluid unit. The fifth complaint of the clothing season is the 'light transmission portion is set in the above-mentioned tightening portion observation: the first aspect on the end portion to the fourth aspect of the fourth aspect of the fluid in the fourth aspect The closer to the downstream side of the tightening portion, the 320011 9 200839211 the tightening of the upper side and the lower side of the lower side are located on the upstream side of the light transmitting portion and the smaller the:::: _ becomes smaller, and can also be > The width difference in the τ direction above the muscle path is also π p thickness thinned. As a result, the total adjacent charge can be set to two; the present invention: 'Πί; 4 result' can correct the particle image by four. The Moonlight 6 is a sample of all the components in the first aspect to the "state of the state - the state of the state" is easily observed through the fluid observation unit and the preparation::: the seventh state is a kind A particle shape analyzing device, in which the fluid observation unit of any aspect of the device is inferior to the F 2 sample to detect the portion included in the foregoing sample: = through the light.丨 “Output and release of particle shape information” The seventh aspect of the sample system can obtain a large number of correct particle and state information in a short time. In Hanoi, ^" accurately measures the shape of these particle images (the effect of the invention) several stability, and the particles in the state of the reset posture are maintained in special and borrowed from the poor, six carvings # y people 〇 汛A mouth shape, a polar fluid observation unit, and a particle shape analysis device for the melon/beam limb detection unit. When the particles in the I product stream in the I product stream are particles of the shape of the elongated 320011 10 200839211 such as fibrous particles, the particles are contained in the sample stream due to, for example, a, a, or a ... stably extending in a planar shape and flowing in the flow path - two toward the flow two]:: and ^, ,,,, and mouth to prevent the aggregation of the particles to make = fruit. ° Direction, and play the role of more accurate form information: two! Since the cross-sectional area of the flow path is reduced, the thickness of the through-flow can be extremely thin, and the position and thickness of the sample flow in the limb-measuring unit can be easily and precisely controlled. [Embodiment] Embodiments of the present invention will be described with & In addition, this embodiment is only an exemplification, and the present invention is defined by (the embodiment is not limited to the description of the embodiment. The yth line indicates the particle shape of the embodiment gl of the present invention (4) 4 :Ϊ: As shown in Fig. 1, the particle shape analysis v = in this embodiment is a dlspersion unit 10, connected to a disperser, and 10 pump unit (clear punit) 20, connected to the pump unit 2〇, (obs(10)tlGnunlt)3G, connected to the observation unit 3G as an analytical analysis unit (analyzing unlt) 40, and a control unit ((3) ntrol unit) 50 connected to all of the groups. In the following state, the observation unit 3 is composed of a sample flow generation unit, a fluid 2: a How cell, and a particle detection unit. The dispersion unit 1 has a particle for analysis. After dispersing the product liquid, the sample liquid is supplied to the function of the pump unit 2 (). For example, ^ 320011 11 200839211, a funnel-shaped supply device pump unit (4) equipped with a stirrer with a powerful stirring function, has a supply Self-dispersing unit 1 () sample liquid • first storage The carrier solution is supplied to the observation unit 3G and has a function of accurately controlling the supply amount of each liquid. The observation unit 30 is shown in Fig. 2, and the sample flow production unit Γ, description = The fluid observation of the light transmitting portion 322 through which the light is transmitted is set as follows: 32, and the makeup portion 33 and the particle detecting portion 34 which are disposed to sandwich the light transmitting portion 322 of the fluid observation unit & The σ and the sample flow creating unit 31 are capable of being supplied to the fluid observation unit by using a sample port supplied from the pump unit 20 = a carrier solution to prepare a sample solution in which the sample solution is encapsulated. ^ / The sample flow producing unit 31 is composed of, for example, a tubular member having a rectangular cross section through which a carrier flows, and a sample liquid nozzle inserted into a central portion of the upstream side of the tubular member and discharging the sample liquid, and capable of The sample liquid is injected into a central portion of the carrier solution for producing the official member, etc. The claw light P 33 is composed of a light source 331 such as a flash lamp and a rotation filter 332: the shutter has a high-speed shutter function. On the other hand, the particle detecting portion 34 D camera 341 and through The composition of 342, and can be seen through the source 331, and the i. + drinking team Chiyou, = shoot the light from the sub-tooth sample, and obtain the particle image, the information is output to the outside. The observation unit 30 can obtain vivid and intra-particle images in a short time and continuously by the second and particle detecting units 34', and output the images to the outside. The analysis unit 40 has a resolution wheel from the observation. The particle shadow of the unit 30 320011 12 200839211 t The diameter and maximum length of the particles contained in the sample stream are separated from the individual. The unit 4G system can be, for example, one of the four functions of the slave computer and a special computer. . Each of the casings 50 has a control signal to be transmitted to each unit to control the control unit 5. For example, the J-Men's computer and a dedicated computer may be provided, and the control unit 50 and the analysis unit 4 () may be provided. Electric moon self-construction \Second, detailed description of the fluid observation unit 32. Fig. 3 is a schematic perspective view of a fluid observation unit of the form: a shape, and Fig. 4 is a longitudinal schematic perspective view of the volume observation unit. The red fluid display unit 32 is composed of a rectangular parallelepiped member composed of a glass genus, and is provided with a rectangular upstream side opening 326' on the left side surface and on the right side surface. A downstream side opening 327 having a flat rectangular shape in the dead direction is provided. Further, in the fluid observation, the inside of the crucible 32 is provided with a squeezing P (throttle) 321 which is formed symmetrically with the center of the flow path 325, so that the flow path 325 connecting the upstream side opening 326 and the downstream side opening J 327 is up and down. The direction is tight. Further, on the downstream side of the tightening crucible 321, a light transmitting portion 322 made of a transparent member such as a transparent acrylic resin or glass is provided. In other words, in the light transmitting portion 322, the flow path is only contracted from the up and down direction. Therefore, by configuring the fluid measuring unit 32' as described above, it is possible to obtain a particle image of the particles contained in the sample stream while obtaining the effect produced by the constricting portion 321 which will be described later. Furthermore, the fluid observation unit 32 can be configured such that the two members formed by the constricted portion 321 are disposed such that the faces of the constricted portions 321 face each other, and the flow path between the two members is formed 320011 13 200839211. The two sides are called plugs of the ridge plate member, etc., ds ° • Here, the tightening portion 321 is formed such that the distance y 从 from the center 4 of the flow path 325 is represented by the LL line. y /, the distance X of the upstream end of the tight portion is inversely reduced, and specifically, it is formed such that the following formula is satisfied, and the lower tightening portion 32 is also the same. y (ax + bj type i is tight:: and b is a knife of a>°, b> 0 any constant, x is the distance from the upstream end of the constricted portion) β is thus able to form the constriction 32 The flow stability of the sample flow is: 2, and when the sample flow flows through the flow path 325, the sample flow is elongated toward the downstream side by the opening 326 toward the upstream side of the flow side) Therefore, the effect of reducing the probability that the plurality of particles are in a state of being overlapped each other is exerted. In other words, for example, if the image of the sample pl is in the state of being overlapped with each other, even if the particles pl are in the state of being overlapped with each other, the sample flow is formed in the up and down direction when the sample stream flows through the flow path 325. It is flat and faces in the outflow direction, and is continuously elongated by a certain degree of addition, so as shown in Fig. 5 (8), the particles contained in the sample stream
Pi與粒+ P2會逐漸被分離。因此，被粒子偵測器料 偵測出粒子pi盥翻^ + + u τ i i 之機率降低。’、 在下方向在互相重疊之狀態下 燮匕卜—在:1:路325内也存在樣品流之流速分布之影 -士同上：4，由於樣品流係在上下方向成型成扁平狀 320011 14 200839211 方向，且以經常一定之加速度持續被拉長， 粒子流入流路325中:向:：而未互相重疊之狀態之2個 _ π/m 會因在流路325内之樣品流之 ，速刀布之4 ’而會發揮使在透光部3 互相重疊之狀態之機率降低之效果。 彳子王 並且321係因形成成使滿足上述之式卜所 會使含於樣品流中之粒子旋轉，即能在流入流 , 〃 使粒子定向成在流路325内最不會受到流體阻 力之安悲而使粒子移動夕— _ 經流路325内時，美於半^ :換§之’由於樣品流在流 了基於牛頓之黏性法則，會從上側之緊縮 4321朝向下側方向經常施加成&比例之一定之力，並且 ^下側之緊縮部321朝向上侧方向經常施加成a比例之 疋之力。其結果，由於對含於樣品流中之粒 及:方向皆經常施加成以例之一定之力，故粒子係會以 =向成在流路中最不會受到流體阻力之姿態移動通過流 路内。 此外，緊縮部321係因形成成使滿足上述之式i，所 =路之截面積會連續地減少。因此，能經由調整透光部 ^ 而使在透光冑322之樣品流之厚度變的極薄， 亚且月㉘易且精密地控制在流體觀測單& & 0之樣品Pi and granule + P2 will gradually separate. Therefore, the probability of detecting the particle pi盥^^ + u τ i i by the particle detector material is reduced. ', in the downward direction in the state of overlapping with each other - in 1: Road 325 also has the effect of the flow velocity distribution of the sample flow - the same as above: 4, because the sample flow system is formed into a flat shape in the up and down direction 320011 14 200839211 The direction is continuously elongated by a constant acceleration, and the particles flow into the flow path 325: 2 _ π / m of the state of :: but not overlapping each other due to the flow of the sample in the flow path 325, the speed knives The cloth 4' exerts an effect of lowering the probability that the light transmitting portions 3 overlap each other. The scorpion king and the 321 are formed so that the particles contained in the sample stream are rotated by satisfying the above formula, that is, in the inflow stream, the particles are oriented so as to be least subjected to fluid resistance in the flow path 325. An sorrow makes the particles move _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ A certain force of the ratio of & and the lower side of the constricted portion 321 is often applied to the upper side direction as a force of a ratio of a. As a result, since the particles contained in the sample stream and the direction are often applied with a certain force, the particle system moves through the flow path in a posture in which the flow direction is least subjected to fluid resistance in the flow path. Inside. Further, since the constricted portion 321 is formed so as to satisfy the above formula i, the cross-sectional area of the path is continuously reduced. Therefore, the thickness of the sample stream in the light-transmitting crucible 322 can be extremely thin by adjusting the light-transmitting portion ^, and the sample of the fluid observation sheet &&
之位置及厚度。 水口口々丨L 、如以上說明’根據粒子形狀分析裝置卜由於能降低 制出複數粒子呈互相重疊之狀態之粒子之機率，並且能 使粒子定向成在流路325中最不會受到流體阻力之姿態而匕 320011 15 200839211 使粒子移動，故能在短時間内取得大量正確的粒子影像， 、亚且能在短時間内測定該等粒子影像之形態資吒。 再者，流體觀測單元32係能依㈣條;等；^靈活地設 •计。在以下，經由表示表示流體觀測單元32之緊縮部321 之形狀之方程式之導出過程，說明其理由。首先 ，所示，若令上游側開口之上下方向之寬度當做H、：從 k路325之上游側開口 326至下游側開口如之長 部321之全長）當做l，並以χ= 〇 又…'、 :卜則可得到下式。 y Μ帶入上述之式 式2 之截面積A及樣品流 v係在位置x之樣品 流與檣面之間不會發 此外’在預定位置X之流路325 之流量Q係由下述之式表示。再者， 流之流速。再者，在此，已設定樣品 生摩擦。 •式3 式4 、A = 2Wy = 2W i_ ax + b Q=Av=2Wy^rb 在此，由於樣品流之流速▽係能由下式表示 Q(ax + b) —2W — · · · · ·式 5 ’表不在位置 326往下游側 故能由將式5以位置x偏微分所得到之下式 X樣品流朝樣品流之流出方向（從上游侧開"口Location and thickness. The water port 々丨L, as explained above, is based on the particle shape analysis device, because it can reduce the probability of producing particles in which the plurality of particles overlap each other, and can orient the particles to be least subjected to fluid resistance in the flow path 325. At the attitude of 匕320011 15 200839211, the particles are moved, so that a large number of correct particle images can be obtained in a short time, and the shape information of the particle images can be measured in a short time. Furthermore, the fluid observation unit 32 can be flexibly designed according to (4); Hereinafter, the reason will be explained via a derivation process indicating an equation representing the shape of the constricted portion 321 of the fluid observation unit 32. First, if the width of the upper side opening of the upstream side opening is made H, the length from the upstream side opening 326 of the k path 325 to the downstream side opening such as the long part 321 is regarded as l, and χ = 〇 again... ', : Bu can get the following formula. y Μ The cross-sectional area A and the sample flow v brought into the above formula 2 are not between the sample stream and the crucible surface at the position x, and the flow rate Q of the flow path 325 at the predetermined position X is as follows. Expression. Furthermore, the flow rate of the flow. Furthermore, here, the sample has been set to generate friction. • Equation 3 Equation 4, A = 2Wy = 2W i_ ax + b Q=Av=2Wy^rb Here, since the flow velocity of the sample stream can be expressed by the following formula Q(ax + b) — 2W — · · · · The equation 5' is not at the downstream side of the position 326, so that the sample X can be obtained by the differential expression of the position x in the direction of the flow out of the sample stream (opening from the upstream side)
3200H 16 200839211 開口 327流動之方向）加速之加速度（平面延伸速 ^δν _Qa 6 dx 2W ••式 6 325内之樣 而是經常一 中，而可得 如此式所示，流經流體觀測單元32之流路 品流之平面延伸速度ε，係得知與位置x無關， 定。因此，經由將式2及式6代入上述之式工 到下式。 ^ •式7 2Ws 另一方面，若令上游側開口 326之截面積當做幻、八 在上游側開口 326之樣品流之流速當做 令 依下式算出。 貝里Q係能 2 = 。· · · ·式8 此外，若令下游側開口 327之截面積當做h，則 游侧開口 327之樣品流之流速W係能由下式表示。下 · · · · ·式 9 於疋，若將式8及式9代入式7，則可得到下式。 2W(A^Aj~2......1 03200H 16 200839211 The direction of the flow of the opening 327) Acceleration acceleration (plane extension speed ^δν _Qa 6 dx 2W • The sample 6 325 is often in the middle, but can be shown in this way, flowing through the fluid observation unit 32 The plane extension speed ε of the flow path product is determined to be independent of the position x. Therefore, by substituting Equations 2 and 6 into the above formula, the following formula is given. ^ • Equation 7 2Ws On the other hand, if The cross-sectional area of the upstream side opening 326 is assumed to be imaginary, and the flow rate of the sample flow at the upstream side opening 326 is calculated as follows. The Berry Q system can be 2 = 。 · · · · Equation 8 In addition, if the downstream side opening 327 When the cross-sectional area is h, the flow velocity W of the sample flow of the swim-side opening 327 can be expressed by the following formula: Next, the formula 9 is in the 疋, and if the formula 8 and the formula 9 are substituted into the formula 7, the next can be obtained. 2W(A^Aj~2...1 0
AxA2L X + Jj 、由此式传知，只要決定流路之全長L、上游侧開口 3 2 6 之截面積Al、下游側開口 327之截面積A2和樣品流之流量 320011 17 200839211 L則流體觀測單元3 2之 •之中央至緊縮部之距離y)係能容易地決之^—5 施形態中所使用之流體顴— 、 換&之，本實 靈活地設計。 “早70 32係能依測定條件等而 縮部321内所形成之透光版喊測早凡32之緊 此，透光邱32? & Ate 係可形成於任何位置。因 定條件“靈活地設計，作以緊 了!之下游側為佳，且特別是以設置於下端二 t越接近緊縮部321之下游側，則上侧之緊二:1 與下側之緊縮部321之距離變 J；^321 部322之上游側與下游側之流路：= =，透光 八、，、°果可使粒子通過CCD攝影機等之焦點範園内， 而發揮能更正確地取得粒子影像之效果。 ， (實施形態2) 在實施形態1中，卜被农壯 软 述形狀之緊縮部321係形成於流 體减別早兀32之上游側開口 326至下游側開口 327之間， 但只要形成於上游側開口 326與下游側開口奶之間即曰可 广置於任何位置。再者’即使為該情形，透光部當然仍必 須設置於緊縮部内。 可舉例如··如第7圖所示，從流路325之上游側開口 326Α(χ 0)至位置1，緊縮部321Α之形狀係形成成樣品流 朝樣品流之流出方向（從上游側開口 326往下游側開口 327Α流動之方向）加速之加速度（平面延伸速度相對 於位置X呈線性增加之形狀，從位置丨至下游側開口 320011 18 200839211 -(x=L)，為了使滿足 •伸速度£’維持_定者等。再者而::成=之平面延AxA2L X + Jj , by this formula, as long as the total length L of the flow path, the cross-sectional area A1 of the upstream side opening 3 26 , the cross-sectional area A2 of the downstream side opening 327, and the flow rate of the sample stream 320011 17 200839211 L are determined. The distance from the center of the unit 3 2 to the tightening portion y) can be easily determined by the fluids used in the form of the method, and the design is flexible. "Early 70 32 series can be determined according to the measurement conditions, etc. The light-transparent version formed in the constricted portion 321 is as early as 32, and the light transmission Qiu 32? & Ate can be formed at any position. Preferably, the downstream side is preferably tighter, and in particular, the closer the second end t is to the downstream side of the tightening portion 321 , the distance between the upper side of the tighter 2:1 and the lower side of the tightening portion 321 is changed. J; ^321 The upstream side and the downstream side of the portion 322: = =, light transmission eight,,, ° fruit can make the particles through the focus of the CCD camera, and play the role of more accurate particle image . (Embodiment 2) In the first embodiment, the constricted portion 321 of the shape of the soft body is formed between the upstream side opening 326 and the downstream side opening 327 of the fluid-reducing early hand 32, but is formed upstream. The side opening 326 and the downstream side opening milk can be placed in any position. Furthermore, even in this case, the light transmitting portion must of course be disposed in the constricted portion. For example, as shown in Fig. 7, from the upstream side opening 326 Α (χ 0) of the flow path 325 to the position 1, the shape of the constricted portion 321 is formed so that the sample flow flows toward the sample flow (opening from the upstream side) 326 acceleration toward the downstream side opening 327 Α) acceleration (the plane extension speed increases linearly with respect to the position X, from the position 丨 to the downstream side opening 320011 18 200839211 - (x=L), in order to satisfy the speed £'Maintenance_determination, etc. Again:: ==
當然會呈連續狀。 ，緊縮部321A . 而且’即使為此情形，I實祐报能， 32同樣地’流體觀測單元32A、传^之流體觀測單元 設計。以下，說明其:由。月匕依測疋條件等而靈活地 首先，說明關於之區域。 ，中，在任何位置X，樣品流朝樣品流之流出°；^之區域 開口㈣往下游側開口 327A流動之方向）向（攸上游側 面延伸速度）ε ’係由下式表示。 k之加速度（平 :Φ dx ε' 式1 在此’由於在此區域中之平面延伸 ， 置X呈線性增加，故也能表示成如下式、。係相對於位 ........式1 2 工 _ I b dx 於是，由於可從式U及式12得到下式， αχ 式1 3 故若將此式以χ積分，域入χ==〇、 —〇時之樣品流之流速）則可得到下式 1Of course it will be continuous. The tightening portion 321A. Moreover, even in this case, I can report the energy, and the same applies to the fluid observation unit 32A and the fluid observation unit. Hereinafter, it will be explained: by. The moon is flexible according to the conditions of the test, etc. First, explain the area. , at any position X, the flow of the sample toward the sample stream; the area of the opening (4) to the downstream side opening 327A direction of flow) (the upstream side extension speed) ε ' is expressed by the following formula. Acceleration of k (flat: Φ dx ε' Equation 1 Here, because of the plane extension in this region, X is linearly increased, so it can also be expressed as follows, with respect to the position.... Equation 1 2 _ I b dx Thus, since the following formula can be obtained from the formula U and the formula 12, αχ is a formula 1 3, so if this equation is integrated by χ, the sample flows into the χ==〇, —〇 Flow rate) can get the following formula 1
V 係表示在V system is expressed in
X 2 似仰❶····.·式14 二之·流::5係表示成…， 320011 19 200839211 故若在此式中代人式14並變形，則可得 yX 2 is like ❶ ❶······· 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14
Q 到下式 2Wi\ ax + vo .....式1 6 其次’說明關於之區域。在b :，因在任何位置X，樣品流之平面延伸速^之區域 疋’所以平面延伸速度ε，係由下式表 "£ ......1 7 ε，維持一 7f\ 〇 dv ε --- zr nr dx 於是，若將此式以 ▲ 可得到下式。 矛貝刀，亚代入式U與x=1，則Q to the following formula 2Wi\ ax + vo ..... Equation 1 6 Next 'Describe the area. In b:, because at any position X, the plane of the sample flow extends the area of the velocity 疋' so the plane extension velocity ε is maintained by the following formula "£...1 7 ε, maintaining a 7f\ 〇 Dv ε --- zr nr dx Thus, if this formula is ▲, the following formula can be obtained. Spear shell knife, sub-input U and x=1, then
V alx + v0--X2.....s 而且，由 2W\ alx + v0 ^~x2 e於若將此並變形，則可得到 ^ · · ·式1 9 ，故若代入 X=L、y=fj, # Q 並變形，則可得到下式 a = ML{L-\l) .....式2V alx + v0--X2.....s Moreover, by 2W\ alx + v0 ^~x2 e, if this is deformed, then ^ · · · Equation 19 can be obtained, so if X=L is substituted, y=fj, # Q and deform, then we can get the following formula a = ML{L-\l) .....
以上，由上述式得知 則流體觀蜊單元32A 樣品流之流量Q等，則达：要決定流路之全長L、位置 之形狀係能容易地決定。換令、τ〜之緊縮部321a 用之 、。之，本實施形態中所使 320011 20 200839211 流體觀測單元32A係與實施形態！之流體觀測單元&同梓 -地，能依測定條件等而靈活地設計。 水 (其他實施形態） •一在實施形態1及2中，緊縮部32卜321Α係形成成使 雨述流路之剖面呈矩形，但只要為形成成使流路之截面積 連縯地減少，且流經流路内之樣品流會穩定地呈平面狀延 伸流動者即可。若滿足此條件，則流路之剖面也可呈例如： ^圓形或梯形等。亚且，在實施形態工及2中，緊縮部如、 321A係僅流路之上下方向(一方向)之寬度產生變化，但也 可使左右方向（其他方向）之寬度產生變化。如此進行，也 到與實施形態i及2同樣之效果。再者，此種緊縮部 之形狀係使用數值計算而算出。 此外，在實施形態i中，如上述以流路挪之中央為 I心形成在上下方向對稱之緊縮部321、32ia，但也可形 2成例如如第8圖所示之流體觀測單元32β，使從下側之 之緊縮部表面至上側之緊縮部之距離滿足 ^射如此進行，也可得到與實施形態1同樣之效果。再 考，對於實施形態2也能同樣地考量。 _ ，在實施形態1中係以透明之丙烯酸樹脂或石英 玻璃專構成透光部，但透光部係，口 物；+旦^ 、 /、要由不會吸收在拍攝 粒子之衫像時使用之波長之光者 構成即可。此外，也可將 構成流體觀測單元32之所有構 ^ , ^ 件乂透明之丙烯酸樹脂或 央玻离寺構成透光部。因如此 能麵总此構成流體觀測單元32,而 幸工易地規測流經流體觀測單元&内之樣品流之流動。 320011 21 200839211 # 【圖式簡單說明】 _ 第1圖係實施形態1之粒子形狀分析裝置— . 第2圖係實施形態1之觀察機組之示意圖。π思圖。 * 第3圖係實施形態1之流體觀測單元之概略、 第4圖係實施形態！之流體觀測單元透現圖。 圖。 峡向概略透視 第5圖（a)及（b)係表示流經實施形態1 士 ，元内之粒子之狀態之概略剖面圖。 之-體觀測單 第6圖係實施形態1之流體觀測單元 透視圖。 爪路之概略 之概:實施形態2之流體觀測單元之上側部分 略j面圖及流體觀測單元内之平面延伸速度之圖式 面圖第8圖係其他實施形態之流體觀測單元之:向::剖 【主要元件符號說明】 10 30 32 32Β 34 50 321Α 322 325 分散機組 觀察機組 流體觀測單元 流體觀測單元 粒子彳貞測部 控制機組 緊縮部 透光部 流路 20 31 32Α 33 40 321 粒子形狀分析裝置 泵機組 樣品流製作部 流體觀測單元 照光部 解析機組 緊縮部 緊縮部 322β透光部As described above, when the flow rate Q of the sample flow of the fluid viewing unit 32A is obtained by the above equation, it is determined that the entire length L of the flow path and the shape of the position can be easily determined. The replacement, τ~ tightening portion 321a is used. In the present embodiment, 320011 20 200839211 fluid observation unit 32A is connected to the embodiment! The fluid observation unit & the same ground can be flexibly designed according to measurement conditions and the like. Water (Other Embodiments) • In the first and second embodiments, the constricted portion 32 is formed such that the cross section of the rain flow path is rectangular, but the cross-sectional area of the flow path is reduced in succession. And the sample flow flowing through the flow path can stably extend in a planar shape. If this condition is satisfied, the cross section of the flow path may also be, for example, a circle or a trapezoid. In the embodiment, the 321A is only changed in width in the vertical direction (one direction) of the flow path, but the width in the left and right direction (other directions) may be changed. In this way, the same effects as in the embodiments i and 2 are obtained. Further, the shape of such a constricted portion is calculated using numerical calculation. Further, in the embodiment i, the constricted portions 321 and 32ia which are symmetric in the vertical direction are formed as the center of the flow path as described above, but the fluid observing unit 32β as shown in Fig. 8 may be formed, for example. The same effect as in the first embodiment can be obtained by performing the above-described distance from the surface of the lower constricted portion to the upper constricted portion. The test can be similarly considered in the second embodiment. _ , in the first embodiment, a transparent acrylic resin or quartz glass is used to form a light-transmitting portion, but the light-transmitting portion is a mouth-like material; + Dan ^, /, is to be used when it is not absorbed in the image of the photographed particles. The light of the wavelength can be formed. Further, all of the constituents constituting the fluid observation unit 32 may be made of a transparent acrylic resin or a central glass-lined temple. As a result, the fluid observation unit 32 is constructed in this way, and the flow of the sample flow flowing through the fluid observation unit & 320011 21 200839211 # [Simple description of the drawing] _ Fig. 1 is a particle shape analyzing device of the first embodiment - Fig. 2 is a schematic view of the observation unit of the first embodiment. π thinking. * Figure 3 is a schematic diagram of the fluid observation unit of the first embodiment, and Fig. 4 is an embodiment! The fluid observation unit is a transparent view. Figure. Schematic diagram of the glacier direction Fig. 5 (a) and (b) are schematic cross-sectional views showing the state of the particles flowing through the embodiment 1 and the element. - Body observation sheet Fig. 6 is a perspective view of the fluid observation unit of Embodiment 1. Outline of the claw path: a schematic view of the upper side of the fluid observation unit of the second embodiment and a plan view of the plane extension speed in the fluid observation unit. Fig. 8 is a fluid observation unit of another embodiment: : Section [Description of main components] 10 30 32 32Β 34 50 321Α 322 325 Dispersing unit observation unit fluid observation unit fluid observation unit particle measurement unit control unit compaction section light transmission section 20 31 32Α 33 40 321 particle shape analysis Device pump unit sample flow production unit fluid observation unit illumination unit analysis unit compaction portion constriction portion 322β light transmission portion
3200H 22 200839211 326 上游側開口 326A 上游侧開口 327 下游側開口 327A 下游側開口 331 光源 332 旋轉濾波器 341 CCD攝影機 342 透鏡 23 3200113200H 22 200839211 326 Upstream side opening 326A Upstream side opening 327 Downstream side opening 327A Downstream side opening 331 Light source 332 Rotary filter 341 CCD camera 342 Lens 23 320011
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| US5861950A (en) * | 1997-07-10 | 1999-01-19 | Particle Measuring Systems, Inc. | Particle detection system utilizing an inviscid flow-producing nozzle |
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