TW200831117A - Shiunko nanomicell for skin treatment and preparation method for the same - Google Patents

Abstract

An improved nanomicell solution of Chinese traditional Shiunko oil is provided, which replace the sesame oil with olive oil and mineral oil as the solvent, and cover the oil particles thereof with hydrophilic layers by nanomicell technology, which make the oil particles as the nano-level particles. The improved nanomicell solution has the advantages of well skin infiltration and non-oily sensation, and excellent effects in bactericidal and wound healing abilities.

Description

200831117 IX. Description of the invention: [Technical field to which the invention pertains] More particularly relates to a method for preparing a purple cloud paste preparation and a preparation method thereof. [Prior Art]

Ziqi ί is a traditional cap preparation, mainly "grass, angelica, sesame oil and yellow butterfly s are II, small sputum, ... the work bait is flat, ^ ΐ is presented ίίΐΐ into ί including shikonin and its derivatives, this - Some ingredients have been proved: f accelerates the growth of meat tissue, shortens the time of inflammation, promotes wound healing,

Hi topical ointment is cheap and easy to make. Long-term and low-cost with sesame oil, oily f material cleaning is often mentioned as the disadvantage of μ Ξ ΐί science from 1968 secret, for the treatment of burning (10) disease containing silver away - day age% ammonium silver soft (S1lver sulfadiazinel%. (10)) is the mainstream, ^ 疋 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王 王Precipitating in the dermis layer causes local skin to appear blue-gray foreign affairs Argyria) 'Even in monolayer culture (m〇n〇iayer cUhure), it is sent to silver ions and sulfadiazine to keratinocytes (keratin〇cytes) and engorgia cells ( Fibroblasts) are highly toxic and delay the healing time. At the same time, the silver attack is quite close to the cells, toxicity, dose and bactericidal dose. It also causes autologous damage during sterilization. In addition, the sulfonamide inhibits bone marrow function. Drugs ^ have an allergic reaction. Nanomicell technology has been developed in recent years. It is a concentrated edge of high-purity phospholipids and solvents (such as glycerin or propylene glycol), which can dissolve oil and is insoluble in normal temperature (25-60 °C). The substance of water. Dilution of the concentrate with water yields the state and size of the vesicles of the closed spherical membrane produced in 200831117. Nano microencapsulation is a kind of transparent soluble liquid which is made by coating a fat-soluble drug into the essence of the coffee. The outer layer is further wrapped with a water layer. The stability is good, the hydrophilicity is high, the physiological safety, the sky wheel stimulation, * use Synthetic surfactants and preservatives, no ethanol, can improve the absorption of active substances and bioavailability. Nano-sized particle size can penetrate cell membranes through cell fusion, inclusion and phagocytosis. It is an ideal drug carrier for a wide range of applications, cosmetics, pharmaceutical preparations and biomedical research. Below the application of the nano-grain, her skin absorption can quickly reach the dermis layer, which can be administered from the skin to provide a non-invasive and effective means of administration. The US patent US6468553 patent case and the Taiwan patent 1226838 patent case all have improved the female matrix of the traditional Ziyun cream, but still do not take off the oily base f. Easy to clean and inconvenient to use, and the phase. The surgery may increase the traditional i-finalization. However, it does not help to increase the penetration of the drug itself into the skin or the affected group. There are ^ traditional sugar paste and Western medicine containing silver ion of the external material ammonium silver ointment = lost 'rabbit Ming people through careful experiment and research, and the spirit of perseverance, the final idea of the case, the following is the case A brief description. SUMMARY OF THE INVENTION The present invention combines the treatment of the scent of the ointment with the novel nano-micro-package, and the different recordings of the eucalyptus oil and the mineral material, the Wei-Mao purple f spoon and the special taste transport: Ya Kejin-step design Gel for application and cleaning =

To reduce the benefits of burning scars and increase the benefits of marriage, and to obtain a high-efficiency sulfonium-silver-purple purple cloud cream nano-package preparation. U Renyou ii conceived 'proposed to treat nano skin microencapsulation for skin diseases, 3 has the following formation /7. Oily substrate' - comfrey extract and - Angelica extract, 200831117 where the comfrey extract and the The extract of Angelica sinensis is obtained by extracting comfrey and angelica using the oily substrate. The oily substrate, the comfrey extract and the angelica extract are coated in a phospholipids layer to form a semi-microcapsule having a particle size of less than 1 nanometer. Preferably, the oily base comprises at least one of sesame oil, mineral oil and olive oil. /, preferably, the nano-micro package is coated with a water layer. Preferably, the lining lipid layer consists of glycerol and a constitutive lipid. Preferably, the skin condition comprises: bruises, abrasions, cuts, burns, dry skin, chapped, ulcers, frostbite and proliferative skin abnormalities. According to another concept of the inventor, a nano-package gel for treating skin diseases is proposed, which comprises the following components: an oily substrate, a comfrey extract, an angelica extract, and an excipient, wherein the comfrey The extract and the Angelica extract are obtained by extracting the comfrey and angelica with the oily extract. The oily substrate, the comfrey extract and the extract of the extract are coated in a layer of phospholipids to form a nano-package having a particle size of less than 100 nm. Preferably, the excipient is one of a gel and a hydrophilic ointment. More preferably, the oily base comprises at least one of sesame oil, mineral oil and eucalyptus oil, and the outer layer of the nano-package is coated with an aqueous layer. Preferably, the phospholipid layer is composed of glycerin and phospholipids. According to the inventors' conception, the method of extracting from the nano-package/texture of the skin preparation process comprises the steps of: (a) extracting a comfrey with an oily substrate and obtaining an extract; (b) The extract is homogenized by adding a phospholipid and water/high speed stirring to the extract, and (C) filtering the extract. Preferably, step (a) further comprises a step of: immersing in the oily substrate for 24 hours at room temperature. 200831117 Preferably, step (a) further comprises a step of 130 to 140 degrees to extract the comfrey and the angelica. The heating of the oily substance is preferably carried out, and the step (c) is carried out using a filtration membrane of the filtration membrane 八 | | 八 八 _ _ M. For the ruts of mineral oil and olive oil, at least one of the oily base packs of step (a). Sesame oil, • It is preferred to add an excipient after filtration, and further, after step (c), the extract is further mixed and stirred. In some cases, the excipient is one of a gel and a hydrophilic ointment. By the above-mentioned preparation method and fat-soluble hydrazine, the penetrating power of the drug on the skin can be improved by the nanocrystallization and the hydrophilization, and it is difficult to clean and save the hard-to-oil substrate. Reference to the embodiment of the sub-β, the embodiment of the method of the invention, the method of preparing the micro-package and the preparation method of the method, and the content thereof will be described by the following 15 examples, but the examples are only preferred. This issue #始二也亚非only! In the preferred embodiments, those skilled in the art will be able to devise other embodiments in the spirit of the invention, which is within the scope of the invention. The comfrey used in our test is the soft comfrey (ie, Arnebia e^cpronia) used by the 'sweetness of the Angelica sinensis (01 iv· ) Diels. First, the preparation method of the different purple cloud paste preparation examples of the present invention will be described, and the effects of the present invention over the prior art will be demonstrated by experimental tests. Example: 200831117 Chinese herbal medicines are prepared with different oily bases, group S·· comfrey 90. Angelica 90 grams, sesame oil 300 grams. 〇 group · comfrey 9 〇 grams, angelica 90 grams, olive oil 3 〇〇 grams. Μ group: comfrey 9 gram grams, angelica 90 grams, mineral oil 3 (10) grams. First, the net impurities of Angelica sinensis were immersed in oil for 24 hours at room temperature, and then heated to 130-140 °C in the induction cooker. The color of the angelica was scorched, and the temperature of the comfrey that was added to the impurities was maintained at 130- At 140 °C for 15 minutes, see the comfrey crisp and easy to break, the oil turns purple, then remove from the induction cooker, remove the angelica and comfrey with the structure, filter the oil with the disinfected 4 layers of gauze. The filtrate is poured into a glass bottle and labeled for use. Example (2) Material of Ziyun cream nano-enveloping liquid: (1) S, 0, M components of Example 1 (2) Glycerin (3) Phospholipid (4) Pure water method: -^Phospholipid added (1) Middle-mixing - pre-step mixture is poured into glycerin and stirred - pure water is added before the mixing step - high-speed emulsification homogenizer high-speed stirring - pouring into high-pressure homogenizer homogenization - 0.1 / zm filter membrane filtration - collecting purple concentrate 200831117 The preparation method of the cream Neil 饊钇 gel falls, Ziyun f nano-package liquid 100cc + transparent condensed simple mixed effect test experiment:

Particle size analysis experiment Instrumentary · Beckman coulter TM N5 particle size analyzer Method · Drop, sample to be tested - droplets, drip in a (10) W secondary water beaker, shake evenly. - Take 2-3 drops of the upper liquid into the transparent cell containing the secondary water - place it in the correct position in the particle size analyzer. 2 Check the dynamic soft II and measure the average value of each product for three times. RESULTS: The sesame oil was obtained by the rapid treatment. The grain of the sesame oil was measured. The bread liquid and the oily liquid of the sylvestre sylvestris were named as Ziyun; Nai; the micro-two physics into the first-graph 'the particle size can be less than just pumping. Through the nano-micro-package technology to the sky, the wire diameter is high. Placed at room temperature 15 Method: divided into no drug-containing control group, drug Gudu: tr; grass bag; seed strain is bacteriostatic test = = 2 τ inner sensation = total number of bacteria in 200831117) Into each of the above six Soy Broth glass bottles, mix and culture under the order: ®^^35Ϊ: Horse blood/woodwood medium (H〇rse B1〇〇(1 A) hours, observe bacterial growth and calculate colonies^ Ubcult (4) 24 Results: The test results of each strain are listed in Tables 3 to 7, and please refer to (A) (B), which is pseud〇monasae_ bacteria (峥's one picture test (6), city ^ (:=== == , , and (1) colony maps for 0, 8 and 24 hours of culture, respectively; , , Ί, ϊ ΐϊ ΐϊ ΐΐ 或 or purulent tissues are the most commonly cultivated strains, easy to sputum, disease and even ¥ to burn the injured patients Death. It can indeed inhibit Pseudomonas aeruginosa. Wang Bei is not clear () Pseudomonas aeruginosa as shown in Table 3: 1, the number of colonies in the minute &; 1 〇 5, 8 hours of colonies 〇, 2 ", 守The number of bacteria is 0, which shows excellent inhibition against pseud〇monas aerugin〇sa. ^ 2, 3. Commercially available π mouth Astragalus membranaceus has no antibacterial activity against pseud〇monas aerUgjnoSa Use. The number of colonies of sesame oil lycopene micro-encapsulated liquid in 〇 minute > 105, the number of colonies in 8 hours 丨 1 solid 24 small days, the number of stagnations 〇 one 'shows the effect of pseud〇m〇nas erUgin〇sa j. The number of colonies of j. sylvestris sylvestris var. chinensis microcapsules in 〇; 1 〇 5, 8 hours of colony, 0 number of 24 hours, showing inhibition of pseu (|omonas aerUgin〇sa 4, 200831117 The number of colonies of Pseudomonas aeruginosa is very good, and the effect of 8 hours of colony is excellent. (2) Escherichia coli is shown in Table 4: 1. Inhibition effect of sulfonium silver on Escherichia coli Excellent 2. • 2. The commercially available Ziyun cream has no antibacterial effect on Escherichiac lig. 3. 紫草丝微包液, olive oil 紫草奈米微包液, 刺奈米微包液 to Escherichia coli No inhibition effect. ', service, Zhuo (^) Enterococcus faecal is as shown in Table 5: 1, sulfonium silver inhibits Enterococcus faecal is extremely effective soil 2, the commercial product Ziyun cream is not bacteriostatic to Enterococcus faecalis 3. 3, sesame oil licorice micro-package, olive oil lavender nano-micro The liquid encapsulation, nano-encapsulation of Enterococcus faecalis is not inhibited by comfrey (4) Proteus mirabilis as shown in Table 6. 1. The inhibitory effect of pyrophyllin silver on Proteus mirabilis is excellent. 2. The commercially available product Ziyun cream has no antibacterial effect on Proteus mirabilis. 3. The sesame oil lycopene micro-encapsulation solution, olive oil lavender nano-package, and storage. Shape, milled oil comfrey 12 200831117 Nano microencapsulation has no inhibitory effect on Proteus mirabilis. (5) Acinetobacter baumannii as shown in Table 7: 1. Shihuangxue silver has excellent inhibitory effect on Acinetobacter baumannii. 2. The commercially available Ziyun cream has no antibacterial effect on Acinetobacter baumannii. 3, sesame oil lycopene micro-encapsulated liquid, eucalyptus oil licorice nano-package liquid, mineral oil comfrey nano-encapsulation effect on Acinetobacter baumannii bacteria is not ^. Political test (3) Burning burns and effective evaluation of experimental animals] 12 rabbits (male New Zealand rabbits, New Zealand methods and procedures: (Attenuation Medical Laboratory Building Experimental Animal Center) _ (1 The establishment of a burn-and-burn model 1. Before the start of the experiment, the rabbits were cleaned with a non-bacterial drug. The back of the rabbit was shaved with an electric scissors. After the hair was dried, the ketamine 40 mg/kg dose was anesthetized. 3, 12 rabbits, divided into 6 groups (A, B rabbits for the first group, C, D a H rabbits, the third group, _!, H rabbit for the fourth group, 丨, · 1 rabbit for the fifth Red κ and ί rabbits are the younger brothers, and), the same group of rabbits applied the same drug parts after the bruises. The direction of change of the direction is applied to the experimental external use ointment =, 1 called sulfadiazinel% cream, city The sales volume is good for this two, ',,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, ‘, 5, after waking up, put it back into the cage of the animal experiment center and raise it at normal room temperature at room temperature. 6. Apply and take photos every day after surgery. Apply once every two days after the third day and take photos until the wound is completely healed. 7. In order to prevent the rabbit from ingesting the drug on the wound and affecting the observation and evaluation of the therapeutic effect, the rabbit is put on the neck cover designed by the rabbit after the dressing change. 108, / After the experiment, 5, 10, 15, 2, 25, and 3 days, each rabbit was sacrificed. 'Surgical scissors were taken and placed in 10% formalin solution for pathology. Slice the pathology specialist to assist in observing histological changes. 9. A total of 36 wounds from the remaining 6 rabbits continued to be applied to the wound healing and observed for scar contracture. (2) Pathological section The rabbits were sacrificed and the skin of each wound was removed. The area of about 3 X 3 cm was fixed in 1%% 10 marinine solution, dehydrated, degreased, embedded, and cut into 3~6// with a microtome. The thickness of m was fixed on a glass slide and stained with 显微镜 & e (Hematoxylin & Eosin) and observed under a microscope. According to Hyperkeratosis, epidermal hyperplasia, Hair pigmentation, hair follicles, Apocrine glands, Smooth muscles, Fibroplasia, Vascular pr〇Hferati 〇rl (degree of neovascularization) 'Collagen orientation' and other changes in tissue structure under the microscope to assess the scar growth after burn and the prognosis of wound contracture, scored (Adam J. Singer et al, 2000) . 14 200831117 Gentleman wants · ν'σ * (1) Assessment of the appearance of wound healing The evaluation of the healing degree of the wound in rabbits is given according to the appearance of the wound. ^, ^ : The sputum has fallen off, the wound has healed, and the score of the person is imaginary. The cockroach has fallen off, the wound π has healed and the sputum has fallen off, and the wound has not healed. ~ Raised at the place. The wound has been knotted ‘, no exudate. The wound partially knots ‘, there is exudate. On the 31st and 37th day after the injury, the score is higher. The higher the total score, please refer to Table 8 and Table 9, respectively, and refer to the third ^麋放佺. Don't take the experiment _κ rabbit's injury σ outside ^^ for the 31st day (4). On the 37th day, the healing ability of the wound coated with nano-microgel was better than that of the fine-grained silver and the commercially available group of yellow gum and evaluated on the 37th day. Paste nano-mineral oil purple, Nai-grade gel to promote wound healing ability 'slightly j = Ziyun t nano-package gel. The wound after healing is flat ^: The nano-package gel is preferred. η见/由13⁄4 Secondly, the commercial product Ziyun cream is based on yellow wax, applied to the wound for cleaning, and the wound seems to be particularly sensitive to pain when changing the dressing (known by the rabbit). _ Skin and rabbit hair dyed purple After the hard to fade, affecting the appearance of appearance and other shortcomings. However, these phenomena were all improved in the wounds using the eucalyptus oil violet cream nano-package gel and the mineral oil purple ribbon 暮 too ^ micro-gel gel. Agriculture (2) Pathological changes Table 11 shows the pathological scores of the fifth day after burn wounds in one of the experimental animals. The scores ranged from the worst scores to the nearest one. And the results of the 'Total Tables' recorded on the 10th, 15th, 20th, 25th, and 30th days after burns are listed in Table 1〇, and also refer to Figure 5(A)_(F ), which are the H&E staining maps of the wound tissue at the optical magnification of 2〇 times in the control group, the sulfonium silver group, the commercial product group, the SSN group, the 0SN group, and the MSN group on the 20th day after the burn. From the results of the staining diagrams in Table 1〇 and Group 15 20083ι117, we can see that: 1. In the tissue section score table on the 5th day, there are five kinds of six dressings ~20, 25, 30 days, even better. The screaming blood goes to the quilt's _ skin group _ can the Ministry of Finance and the silk before the end of the ^ ^ micro 2. Age-receiving six records of the wounds and scars _ Taibao broken three groups are better than the control group , sulfonium silver group and commercial products. No. 3. Nano-micro-gel group found in the mineral oil (four) cream nano-package gel followed by sesame oil purple in summary, we can see that the three kinds of sesame oil produced by the invention = nano micro The liquid encapsulation can still be kept below the vast area after being placed at room temperature for a long time. It has excellent s-suppressing ability 4 in Ziyun f nano-packaged silver soft reward. In addition, the wound is easy to clean and the healing is not purple. ～~~~~~~~~~~~~~~~~~~~~~~~~~~^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ The gel scar was found to be the result of mineral oil Ziyun cream nano-microgel gel re-extraction 2, followed by sesame oil-purple nai # to illustrate the hair and its efficacy, __ the present invention. The micro-packaged three, _ is better than the ship group, the frequency silver group and the city money grass paste. The above embodiments may be modified and changed without departing from the spirit of the present invention. The scope of the invention should be as set forth in the appended claims. • References: 1. Adam J. Singer, Henry C. Thode Jr. 5 Steve A. McClain. Development of a histomorphologic scale to quantify cutaneous scars after burns. Acad Emerg Med. 2000,7(10):1083-1088 17 200831117 Chart description: Table 1 Particle size data table sesame oil Ziyun lean nano-package liquid olive oil Ziyun cream nano micro-package mineral oil Ziyun cream nano-micro: liquid on the 01st day 66. 6±25. 8 nm 80· 5土25· 5 nm 70. 8±27. 7 nm Day 20 99. 0+31. 6 nm 79.1±25. 0 nm 44. 7+20. 4 nm Day 30 78. 7±27· 5 nm 91. 2+30. 5 nm 75. 8±26. 7 nm Day 40 71·9±22.4 nm 78. 3+23. 4 nm 80.1+24. 6 nm Day 50 76. 2+22. 7 nm 80. 7±26. 9 nm 92. 9±31.2 nm Day 60 73· 4±29· 4 nm 90· 2 soil 30· 9 nm 66. 4+23. 2 nm Day 150 86·9±40· 9 nm 89.1+28. 8 nm 84. 0±24, 6 nm Table 2 is the five species of bacteria that are tested and suppressed! Pseudomonas species No. Pseudomonas aerug i nosa (Pseudomonas aeruginosa) NCTC 10662 Escherichia coli (Escherichia coli) ATCC 35218 Enterococcus faecal is (Enterococcus faecalis) ATCC 29212 Proteus rairabilis (Proteus) ATCC 7002 Acinetobacter baumannii Bacillus) ATCC 19606 18 200831117

Table 3 NCTC 10662 Pseudomonas aeruginosa Colony number control group Sulfide silver commercial product SSN 0SN MSN 0 minutes > I05 > 105 > 105 > I05 > 105 > 105 8 hours > 105 0 > 105 5 10 26 24 hours> 105 0 > ίο5 0 0 0 Table 4 ATCC 35218 Escherichia coli Colony number control group Shihuang Qianyin commercial item SSN 0SN MSN 0 minutes> 105 > 105 > 105 > 105 > 105 > 105 8 hours > 105 0 > 105 > 105 > 105 > 105 24 hours > 105 0 > 105 > 105 > 105 > 105 Table 5 ATCC 29212 Enterococcus faecal is Colony number control group Shihuang Qianyin Commercial SSN 0SN MSN 0 minutes > 105 > 105 > 105 > 105 > 105 > 105 8 hours > 105 0 > 105 > 105 > 105 > 105 24 hours > 105 0 > 105 > 105 > 105 > 105

19

200831117 Table 6 __ ATCC 7002 Proteus mirabilis ^^ falling control group Shihuang in An Yin 0 minutes > 105 > 105 8 hours > 105 0 24 hours > 105 0 Table 7 ATCC 19606 Acinetobacter control group Shihuang in An Yin 0 minutes > 105 > 105 > 105 8 hours > 105 0 > 105 24 hours > 105 0 > 105 ® Table 8 Rating on the 31st day after burn

On the 31st day, the control group Silver sulfadiazine 1% cream commercially available Ziyun cream hemp; T nano-micro- is glue----- 撖油油紫云膏奈; 句句凝胶矿物油紫云膏奈米撒句凝蓼A rabbit 2 2 1 2 2 2 B rabbit 1 3 2 2 9 9 Η rabbit 1 3 2 —-------- 2 L 5 L 2 J rabbit 2 2 2 4 5 2 Κ rabbit 2 2 2 4 5 5 L rabbit 2 2 2 4 2 2 Total 10 14 12 -------- 18 23 15 Average 1.67 soil 0. 52 2. 33±0. 52 2±0 2. 83+1. 33 3.50±1.64 2. 50±1. 22 20 200831117

Day 37 Control Silver sulfadiazine 1% cream

Sesame oil purple cloud lean nano micro-gel olive oil purple cloud cream nano micro-encapsulated gel mineral oil purple cloud cream nano micro-package gel 4 5 2 3 5 4 5 5 5 5 5 4 26 26 4. 33±1.21 4. 33± 0. 81 _10 纾! Scale summary

Dressing 1: Control group dressing 4: paralysis (4) micro-package gel Na · olive oil nano-package gel dressing 6 · mineral oil purple S cream nano-package _. 21 200831117

s J„ )H<LO姝啭¥叙^翱龄韧螺 s J„ ) <9 姝 total score CO LO CO OO Collagen tissue growth degree Κ - -Μ Μ -3⁄4 埘姐_噼费斗铋_ 2刼鲶 Η 刼 刼 刼 命 命 命 命 SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW SW > i <N1 - Mail CD Beer <ZD rH TH τ-Η οα 1 ( C<1 1—H 03 oo Neovascular proliferation degree s \^/ tH C3> CD CD CD 7-1 璀c fiber The degree of blast proliferation 枇s CD 〇ο CD CD T1·' t smooth muscle 杷 s τ-1 t-H CD τ--Η CD r-H apocalyptic s C3> CD CD CD 〇) 〇> hair follicle 杷r —H r—H r—Η τ—H r—H ft 墀Second epidermal hyperplasia 杷st—H r—4 τ—4 tH τ—H rH 墀Q Epidermal hyperkeratosis degree cup sr—I tH τ—Η r _H τ—H r—H alkali Q score item dressing 1 dressing 2 dressing 3 dressing 4 dressing 5 dressing 6 to gallium first 3⁄4 come to you 4_铋谀荽襻:9 real. #嫁初«呆. Such as _铋谀韬«: 1-0 real 鹌. s 窫 你 you like #铋号_ : . Such as S 铋 柃: coisl. w«¥:2*^. Disintegration ττ 200831117 [Simple description of the diagram] The first picture: the particle size analysis line of the Ziyun cream nano-package liquid; The second picture (A): Pseudomonas aeruginosa bacteria in the control group (a), sulfonium silver Group (b) and commercial group (c) were colonized at 0, 8 and 24 hours in each culture; Figure 2 (B): Pseudomonas aeruginosa in SSN group (d), 0SN group (e) and MSN group (f) Colony maps at 0, 8 and 24 hours of culture; • Figure 3: Control group (a), sulfonium silver group (b), commercial group (c), SSN on day 31 after burn injury in rabbits Group (d), 0SN group (e) and MSN group (f) Appearance of the wound (the dotted circle is the original burned area); The fourth picture: the control group (a), the sulfonium silver group on the 37th day after the burn (b), commercial group (c), SSN group (d), 0SN group (e) and MSN group (f) appearance of the wound (the dotted circular frame is the original burned area); and the fifth figure (A) - (F): Η & E staining of the wound tissue at the optical multiple of 20 10 times in the control group, the sulfonium silver group, the commercial product group, the SSN group, the 0SN group and the MSN group on the 20th day after the burn. [Main component symbol description] None 23

Claims (1)

200831117 X. Patent application scope: 1. A nano-package solution for treating skin diseases, comprising the following components: an oily substrate; a comfrey extract obtained by extracting and filtering a comfrey through the oily substrate; The extract of Angelica sinensis is obtained by extracting and filtering the Angelica sinensis via the oily substrate, wherein the oily substrate, the comfrey extract and the extract of Angelica sinensis are coated in a layer of phospholipids to form a particle size of less than 100. One of the nano-micro packets of nano. 2. The nanocapsule according to item 1 of the patent application, wherein the oily base comprises at least one of sesame oil, mineral oil and eucalyptus oil. 3. The nano-package liquid of claim 1 of the patent scope, wherein the nano-package is further coated with an aqueous layer. 4. The nanocapsule according to item 1 of the patent application, wherein the phospholipid layer is composed of glycerin and sarcophagus lipid. 5·=Application of the nanometer microencapsulation liquid of the first paragraph of the patent scope, wherein the skin disease package t ^ bruises, abrasions, knife wounds, burns, dry skin, chapped, > shell, neck, and proliferative Abnormal skin. a nano-package gel for treating skin diseases, which comprises the following components: an oily substrate; a comfrey extract obtained by extracting a comfrey through the oily substrate; , which is obtained by extracting an angelica via the oily substrate; and extracting the oily substrate, the comfrey extract and the rim of the stalk; and forming a layer of ph〇sph〇iipids One nanometer micro-package with a particle size of less than 100 nm. 6 items of nano-package gel, the towel of which is one of the excipients of the horse shouting % and a hydrophilic ointment. 24 200831117 8· ί. The nano-package gel of claim 6 wherein the oily base comprises at least one of sesame oil, mineral oil and eucalyptus oil. 9. The nano-microgel/gel according to item 6 of the patent application, wherein the nano-pack is coated with an aqueous layer. 10. The nano-microgel gel of claim 6, wherein the phospholipid shell layer is composed of glycerin and sarcophagus lipid. 11 is a method for preparing a nano-encapsulated liquid for treating skin diseases, wherein the step (a) utilizes oily matrix extraction - comfrey and - angelica to obtain - extract, (8) into a phospholipid and water, high speed and high pressure stirring The draw liquid is homogenized; and (C) the extract is filtered. In the method of preparing the nano-encapsulation liquid in the case of Niu Shen J, the step A (a) further includes a step of 2 in the oily substrate for 24 hours. *Because the temperature is first immersed in 13 · As in the scope of application patent section u ', step (a) further includes a step: sputum method, 13G ~ 14G degrees to extract the comfrey and the ^ ^ (four) sex The substance is heated to 14 · as in the scope of the application of the nth item - (6) is the method of making (4) the diameter of the liquid encapsulation 'the 15', the oily substrate containing the mf encapsulation in the step (a) of the application The method 'its one of them less. , 匕 3 屣 oil, mineral oil and olive oil to 16 · If the step (c) of the patent application scope further comprises a step of the rice micro-encapsulation method, the extract is mixed and stirred. Incorporating the excipient into the filtered 17· as in the scope of claim 17 of the patent, the excipient is a gel and a method of ^ ^ f彳 political encapsulation, and its hydrophilic soft greed One. 25 200831117 VII. Designated representative map: (1) The representative representative of the case is: (). (2) A brief description of the symbol of the representative figure: None 8. If there is a chemical formula in this case, please disclose the chemical formula that best shows the characteristics of the invention: