TW200732475A - Pulmonary stem cells, related methods and kits - Google Patents

Pulmonary stem cells, related methods and kits

Info

Publication number
TW200732475A
TW200732475A TW095138019A TW95138019A TW200732475A TW 200732475 A TW200732475 A TW 200732475A TW 095138019 A TW095138019 A TW 095138019A TW 95138019 A TW95138019 A TW 95138019A TW 200732475 A TW200732475 A TW 200732475A
Authority
TW
Taiwan
Prior art keywords
stem cells
methods
pulmonary
cells
cultivation
Prior art date
Application number
TW095138019A
Other languages
Chinese (zh)
Inventor
John Yu
Alice Yu
Thai-Yen Ling
Original Assignee
Academia Sinica
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/252,458 external-priority patent/US20070087332A1/en
Application filed by Academia Sinica filed Critical Academia Sinica
Publication of TW200732475A publication Critical patent/TW200732475A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0688—Cells from the lungs or the respiratory tract
    • C12N5/0689—Stem cells; Progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/12011—Bunyaviridae
    • C12N2760/12111—Hantavirus, e.g. Hantaan virus
    • C12N2760/12151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/20011—Coronaviridae
    • C12N2770/20051—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Pulmonology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Methods for the isolation, cultivation, identification, and utilization of pulmonary stem cells are provided. Pulmonary stem cells are derived from mammalian lung tissue, being resident therein. Isolation methods include the identification of putative stem cells by their emergence in primary cultures as discrete slow growing colonies. Confirmation of identity as stem cells is by stem cell markers, such as Oct-4, and by their ability to differentiate into a mature phenotype. Isolation and selective methods further include cell sorting on the basis of stem cell markers. Methods of cultivation whereby stem cell populations are expanded while maintaining the undifferentiated phenotype include the use of defined culture media, culture surface modifications, and co-cultivation with pulmonary stroma cells. Methods for the use of pulmonary stem cells are further provided, for studies of the biology of the cells, including viral infectivity, production of virus, and the testing of anti-infective agents.
TW095138019A 2005-10-17 2006-10-16 Pulmonary stem cells, related methods and kits TW200732475A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/252,458 US20070087332A1 (en) 2005-10-17 2005-10-17 Pulmonary stem cells, related methods and kits of parts
US82426506P 2006-08-31 2006-08-31

Publications (1)

Publication Number Publication Date
TW200732475A true TW200732475A (en) 2007-09-01

Family

ID=37963169

Family Applications (1)

Application Number Title Priority Date Filing Date
TW095138019A TW200732475A (en) 2005-10-17 2006-10-16 Pulmonary stem cells, related methods and kits

Country Status (5)

Country Link
EP (1) EP1960553A2 (en)
JP (1) JP2009514509A (en)
AU (1) AU2006304390A1 (en)
TW (1) TW200732475A (en)
WO (1) WO2007047581A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2321406B1 (en) * 2008-08-05 2014-12-10 Keio University Method for selecting secondary neurosphere derived from induced pluripotent stem cell
WO2010088230A2 (en) * 2009-01-30 2010-08-05 Kci Licensing, Inc. Adult stem cell assays
US20120094304A1 (en) * 2009-04-17 2012-04-19 Tohoku University Method of preparing human lung tissue stem cells and method of inducing differentiation into human alveolar epithelial cells
EP2438159B1 (en) 2009-05-29 2018-10-03 Kyoto University Method for selecting clone of induced pluripotent stem cells
US9399758B2 (en) 2009-07-15 2016-07-26 Mari Dezawa SSEA3(+) pluripotent stem cell that can be isolated from body tissue
US9550975B2 (en) 2009-07-15 2017-01-24 Mari Dezawa SSEA-3 pluripotent stem cell isolated from body tissue
WO2011103359A2 (en) 2010-02-17 2011-08-25 Inq Biosciences Corporation Culture systems, apparatus, and related methods and articles
CN102998441A (en) * 2012-11-20 2013-03-27 哈德逊（天津）生物技术有限责任公司 Kit for quickly detecting induced pluripotent stem cells

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003523166A (en) * 1998-09-29 2003-08-05 ガミダ セル リミテッド Methods for controlling proliferation and differentiation of stem and progenitor cells
US7575924B2 (en) * 2000-11-13 2009-08-18 Research Development Foundation Methods and compositions relating to improved lentiviral vectors and their applications
AU2002322857A1 (en) * 2001-08-01 2003-02-17 Jewish Hospital Healthcare Services, Inc. Cellular compositions which facilitate engraftment of hematopoietic stem cells while minimizing the risk of gvhd
GB2398079B (en) * 2001-11-09 2006-05-31 Es Cell Int Pte Ltd Characterization and isolation of subsets of human embryonic stem cells (HES) and cells associated or derived therefrom
AU2003216822A1 (en) * 2002-03-19 2003-10-08 University Of Sheffield Stem cell culture
US20040170954A1 (en) * 2003-02-10 2004-09-02 Mckenney Keith Pathogen inactivation assay

Also Published As

Publication number Publication date
JP2009514509A (en) 2009-04-09
WO2007047581A3 (en) 2009-05-14
AU2006304390A1 (en) 2007-04-26
EP1960553A2 (en) 2008-08-27
WO2007047581A2 (en) 2007-04-26

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