GB2398079B - Characterization and isolation of subsets of human embryonic stem cells (HES) and cells associated or derived therefrom - Google Patents

Characterization and isolation of subsets of human embryonic stem cells (HES) and cells associated or derived therefrom

Info

Publication number
GB2398079B
GB2398079B GB0412528A GB0412528A GB2398079B GB 2398079 B GB2398079 B GB 2398079B GB 0412528 A GB0412528 A GB 0412528A GB 0412528 A GB0412528 A GB 0412528A GB 2398079 B GB2398079 B GB 2398079B
Authority
GB
United Kingdom
Prior art keywords
hes
subsets
characterization
isolation
embryonic stem
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB0412528A
Other versions
GB2398079A (en
GB0412528D0 (en
Inventor
Martin Frederick Pera
Andrew Laslett
Susan Hawes
Tomonobu Gion
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ES Cell International Pte Ltd
Original Assignee
ES Cell International Pte Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AUPR8781A external-priority patent/AUPR878101A0/en
Priority claimed from AUPS1129A external-priority patent/AUPS112902A0/en
Application filed by ES Cell International Pte Ltd filed Critical ES Cell International Pte Ltd
Priority to GB0602441A priority Critical patent/GB2419890A/en
Publication of GB0412528D0 publication Critical patent/GB0412528D0/en
Publication of GB2398079A publication Critical patent/GB2398079A/en
Application granted granted Critical
Publication of GB2398079B publication Critical patent/GB2398079B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
GB0412528A 2001-11-09 2002-11-11 Characterization and isolation of subsets of human embryonic stem cells (HES) and cells associated or derived therefrom Expired - Fee Related GB2398079B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB0602441A GB2419890A (en) 2001-11-09 2002-11-11 Characterization and isolation of subsets of human embryonic stem cells (HES)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AUPR8781A AUPR878101A0 (en) 2001-11-09 2001-11-09 Characterization and isolation of subsets of human embryonic stem cellss (HES) and cells associated or derived therefrom
AUPS1129A AUPS112902A0 (en) 2002-03-15 2002-03-15 Characterisation and isolation of subsets of human embryonic stem cells and progenitor cells derived therefrom (2)
PCT/AU2002/001534 WO2003040355A1 (en) 2001-11-09 2002-11-11 Characterization and isolation of subsets of human embryonic stem cells (hes) and cells associated or derived therefrom

Publications (3)

Publication Number Publication Date
GB0412528D0 GB0412528D0 (en) 2004-07-07
GB2398079A GB2398079A (en) 2004-08-11
GB2398079B true GB2398079B (en) 2006-05-31

Family

ID=25646839

Family Applications (2)

Application Number Title Priority Date Filing Date
GB0602441A Withdrawn GB2419890A (en) 2001-11-09 2002-11-11 Characterization and isolation of subsets of human embryonic stem cells (HES)
GB0412528A Expired - Fee Related GB2398079B (en) 2001-11-09 2002-11-11 Characterization and isolation of subsets of human embryonic stem cells (HES) and cells associated or derived therefrom

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB0602441A Withdrawn GB2419890A (en) 2001-11-09 2002-11-11 Characterization and isolation of subsets of human embryonic stem cells (HES)

Country Status (4)

Country Link
EP (1) EP1448763A4 (en)
CA (1) CA2466342A1 (en)
GB (2) GB2419890A (en)
WO (1) WO2003040355A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7033831B2 (en) 2001-12-07 2006-04-25 Geron Corporation Islet cells from human embryonic stem cells
WO2005033126A1 (en) * 2003-10-09 2005-04-14 Monash University Cell surface marker
WO2005097977A2 (en) * 2004-04-01 2005-10-20 Wisconsin Alumni Research Foundation Differentiation of stem cells to endoderm and pancreatic lineage
WO2006084314A1 (en) * 2005-02-09 2006-08-17 Australian Stem Cell Centre Limited Stem cell populations and classification system
AU2006304390A1 (en) * 2005-10-17 2007-04-26 Academia Sinica Pulmonary stem cells, related methods and kits
WO2007149182A2 (en) 2006-06-19 2007-12-27 Geron Corporation Differentiation and enrichment of islet-like cells from human pluripotent stem cells
CN102803472B (en) * 2009-06-25 2014-04-23 吉隆公司 Differentiated pluripotent stem cell progeny depleted of extraneous phenotypes
US20150175698A1 (en) * 2012-05-22 2015-06-25 Life Technologies As Recombinant antibody compositions and methods of use thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997008295A1 (en) * 1995-08-25 1997-03-06 Lifecell Corporation Reconstituted skin
WO2000027995A1 (en) * 1998-11-09 2000-05-18 Monash University Embryonic stem cells
WO2001068815A1 (en) * 2000-03-14 2001-09-20 Es Cell International Pte Ltd Embryonic stem cells and neural progenitor cells derived therefrom
WO2001072970A2 (en) * 2000-03-28 2001-10-04 University Of Iowa Research Foundation Methods to prepare and use epidermal stem cells
WO2001098463A1 (en) * 2000-06-20 2001-12-27 Es Cell International Pte Ltd Method of controlling differentiation of embryonic stem (es) cells by culturing es cells in the presence of bmp-2 pathway antagonists

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0739412B1 (en) * 1993-12-23 2002-02-27 Infigen, Inc. ungulate EMBRYONIC STEM CELLS AS NUCLEAR DONORS AND NUCLEAR TRANSFER TECHNIQUES TO PRODUCE CHIMERIC AND TRANSGENIC ANIMALS

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997008295A1 (en) * 1995-08-25 1997-03-06 Lifecell Corporation Reconstituted skin
WO2000027995A1 (en) * 1998-11-09 2000-05-18 Monash University Embryonic stem cells
WO2001068815A1 (en) * 2000-03-14 2001-09-20 Es Cell International Pte Ltd Embryonic stem cells and neural progenitor cells derived therefrom
WO2001072970A2 (en) * 2000-03-28 2001-10-04 University Of Iowa Research Foundation Methods to prepare and use epidermal stem cells
WO2001098463A1 (en) * 2000-06-20 2001-12-27 Es Cell International Pte Ltd Method of controlling differentiation of embryonic stem (es) cells by culturing es cells in the presence of bmp-2 pathway antagonists

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
J. Cell Sci., Vol.111, 1998, Lyle, S. et al., "The C8/144B monoclonal antibody...", pp.3179-3188 *
J. Cell Sci., Vol.113, 2000, Pera, M. F. et al., "Human embryonic stem cells", pp.5-10 *
Nature Biotech., Vol.18, 2000, Reubinoff, B. E. et al., "Embryonic stem cell lines...", pp.399-404 *
Nature Biotech., Vol.20, 2002, Richards, M. et al., "Human feeders support prolonged...", pp.933-936 *

Also Published As

Publication number Publication date
GB2398079A (en) 2004-08-11
GB0412528D0 (en) 2004-07-07
EP1448763A4 (en) 2006-11-29
GB0602441D0 (en) 2006-03-22
EP1448763A1 (en) 2004-08-25
CA2466342A1 (en) 2003-05-15
WO2003040355A1 (en) 2003-05-15
GB2419890A (en) 2006-05-10

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Legal Events

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PCNP Patent ceased through non-payment of renewal fee

Effective date: 20201111