WO2007047581A3 - Pulmonary stem cells, related methods and kits - Google Patents

Pulmonary stem cells, related methods and kits Download PDF

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Publication number
WO2007047581A3
WO2007047581A3 PCT/US2006/040373 US2006040373W WO2007047581A3 WO 2007047581 A3 WO2007047581 A3 WO 2007047581A3 US 2006040373 W US2006040373 W US 2006040373W WO 2007047581 A3 WO2007047581 A3 WO 2007047581A3
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WO
WIPO (PCT)
Prior art keywords
stem cells
methods
pulmonary
cells
cultivation
Prior art date
Application number
PCT/US2006/040373
Other languages
French (fr)
Other versions
WO2007047581A2 (en
Inventor
John Cheng-Po Yu
Alice Lin-Tsing Yu
Thai-Yen Ling
Original Assignee
Academia Sinica
John Cheng-Po Yu
Alice Lin-Tsing Yu
Thai-Yen Ling
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/252,458 external-priority patent/US20070087332A1/en
Application filed by Academia Sinica, John Cheng-Po Yu, Alice Lin-Tsing Yu, Thai-Yen Ling filed Critical Academia Sinica
Priority to JP2008536712A priority Critical patent/JP2009514509A/en
Priority to EP06826023A priority patent/EP1960553A2/en
Priority to AU2006304390A priority patent/AU2006304390A1/en
Publication of WO2007047581A2 publication Critical patent/WO2007047581A2/en
Publication of WO2007047581A3 publication Critical patent/WO2007047581A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0688—Cells from the lungs or the respiratory tract
    • C12N5/0689—Stem cells; Progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/12011—Bunyaviridae
    • C12N2760/12111—Hantavirus, e.g. Hantaan virus
    • C12N2760/12151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/20011—Coronaviridae
    • C12N2770/20051—Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Pulmonology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Methods for the isolation, cultivation, identification, and utilization of pulmonary stem cells are provided. Pulmonary stem cells are derived from mammalian lung tissue, being resident therein. Isolation methods include the identification of putative stem cells by their emergence in primary cultures as discrete slow growing colonies. Confirmation of identity as stem cells is by stem cell markers, such as Oct-4, and by their ability to differentiate into a mature phenotype. Isolation and selective methods further include cell sorting on the basis of stem cell markers. Methods of cultivation whereby stem cell populations are expanded while maintaining the undifferentiated phenotype include the use of defined culture media, culture surface modifications, and co-cultivation with pulmonary stroma cells. Methods for the use of pulmonary stem cells are further provided, for studies of the biology of the cells, including viral infectivity, production of virus, and the testing of anti-infective agents.
PCT/US2006/040373 2005-10-17 2006-10-16 Pulmonary stem cells, related methods and kits WO2007047581A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2008536712A JP2009514509A (en) 2005-10-17 2006-10-16 Lung stem cells and related methods and kits
EP06826023A EP1960553A2 (en) 2005-10-17 2006-10-16 Pulmonary stem cells, related methods and kits
AU2006304390A AU2006304390A1 (en) 2005-10-17 2006-10-16 Pulmonary stem cells, related methods and kits

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US11/252,458 2005-10-17
US11/252,458 US20070087332A1 (en) 2005-10-17 2005-10-17 Pulmonary stem cells, related methods and kits of parts
US82426506P 2006-08-31 2006-08-31
US60/824,265 2006-08-31

Publications (2)

Publication Number Publication Date
WO2007047581A2 WO2007047581A2 (en) 2007-04-26
WO2007047581A3 true WO2007047581A3 (en) 2009-05-14

Family

ID=37963169

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/040373 WO2007047581A2 (en) 2005-10-17 2006-10-16 Pulmonary stem cells, related methods and kits

Country Status (5)

Country Link
EP (1) EP1960553A2 (en)
JP (1) JP2009514509A (en)
AU (1) AU2006304390A1 (en)
TW (1) TW200732475A (en)
WO (1) WO2007047581A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2321406B1 (en) * 2008-08-05 2014-12-10 Keio University Method for selecting secondary neurosphere derived from induced pluripotent stem cell
WO2010088230A2 (en) * 2009-01-30 2010-08-05 Kci Licensing, Inc. Adult stem cell assays
US20120094304A1 (en) * 2009-04-17 2012-04-19 Tohoku University Method of preparing human lung tissue stem cells and method of inducing differentiation into human alveolar epithelial cells
EP2438159B1 (en) 2009-05-29 2018-10-03 Kyoto University Method for selecting clone of induced pluripotent stem cells
US9399758B2 (en) 2009-07-15 2016-07-26 Mari Dezawa SSEA3(+) pluripotent stem cell that can be isolated from body tissue
US9550975B2 (en) 2009-07-15 2017-01-24 Mari Dezawa SSEA-3 pluripotent stem cell isolated from body tissue
WO2011103359A2 (en) 2010-02-17 2011-08-25 Inq Biosciences Corporation Culture systems, apparatus, and related methods and articles
CN102998441A (en) * 2012-11-20 2013-03-27 哈德逊（天津）生物技术有限责任公司 Kit for quickly detecting induced pluripotent stem cells

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030008374A1 (en) * 2000-11-13 2003-01-09 Research Development Foundation Methods and compositions relating to improved lentiviral vectors and their applications
WO2003040355A1 (en) * 2001-11-09 2003-05-15 Es Cell International Pte Ltd Characterization and isolation of subsets of human embryonic stem cells (hes) and cells associated or derived therefrom
WO2003080816A2 (en) * 2002-03-19 2003-10-02 University Of Sheffield Stem cell culture
US20040170954A1 (en) * 2003-02-10 2004-09-02 Mckenney Keith Pathogen inactivation assay
US20050118142A1 (en) * 2001-08-01 2005-06-02 Ildstad Suzanne T. Cellular compositions which facilitate engraftment of hematopoietic stem cells while minimizing the risk of gvhd
US20050214262A1 (en) * 1998-02-17 2005-09-29 Tony Peled Methods of controlling proliferation and differentiation of stem and progenitor cells

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050214262A1 (en) * 1998-02-17 2005-09-29 Tony Peled Methods of controlling proliferation and differentiation of stem and progenitor cells
US20030008374A1 (en) * 2000-11-13 2003-01-09 Research Development Foundation Methods and compositions relating to improved lentiviral vectors and their applications
US20050118142A1 (en) * 2001-08-01 2005-06-02 Ildstad Suzanne T. Cellular compositions which facilitate engraftment of hematopoietic stem cells while minimizing the risk of gvhd
WO2003040355A1 (en) * 2001-11-09 2003-05-15 Es Cell International Pte Ltd Characterization and isolation of subsets of human embryonic stem cells (hes) and cells associated or derived therefrom
WO2003080816A2 (en) * 2002-03-19 2003-10-02 University Of Sheffield Stem cell culture
US20040170954A1 (en) * 2003-02-10 2004-09-02 Mckenney Keith Pathogen inactivation assay

Also Published As

Publication number Publication date
JP2009514509A (en) 2009-04-09
TW200732475A (en) 2007-09-01
AU2006304390A1 (en) 2007-04-26
EP1960553A2 (en) 2008-08-27
WO2007047581A2 (en) 2007-04-26

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