TW200533300A - Composition for inhibting dietary lipid absorption - Google Patents

Abstract

It is intended to provide a composition having an excellent effect of inhibiting lipid absorption which can inhibit the absorption of lipids taken by meals and contribute to the efficient discharge of the lipids from the body. This composition is characterized by containing (1) chitosan and (2) at least one water-soluble dietary fiber selected from the group consisting of guar gum, pectin, tara gum and locust bean gum.

Description

200533300 IX. Description of the invention: [Technical field to which the invention belongs; j TECHNICAL FIELD The present invention relates to a composition that can hinder the absorption of fat 5 contained in the diet, so it can be expected to have antihyperlipidemia and antiobesity effects. .

Γ «tr J BACKGROUND OF THE INVENTION BACKGROUND OF THE INVENTION In recent years, with the westernization of dietary life in Japan, the intake of fat (especially an animal-like lin) in Japanese has increased day by day. Based on the nutritional adjustment for 2 years: the result (Ministry of Health, Labour and Welfare), the ratio of calories from fat to calorie intake: (ie, fat-to-calorie ratio) in the age group of _, is more suitable for adults "ratio 25% of the upper limit. Drinking § Increased fat intake is associated with morbidity, coronary artery disease = associated with the onset of obesity, circulatory system diseases, and arteriosclerosis. Money infarction and occupational congestion, etc.) and sugar guilty of puerpera are risk factors that cause circulatory system diseases, systemic diseases, and diabetes. Even by the weight of the problem in the field of prevention. The treatment of materials has also become a medical treatment. As mentioned above, the method of inhibiting or increasing the subsequent comorbidities and the accompanying obesity is accompanied by obesity. It is known that there are methods to control diet to control appetite (rate generation °° method, administration of appetite suppression to take calories, _method = ^) to improve the inserted 1 exercise therapy through exercise) and so on. In fact, these methods are also adopted as countermeasures for obesity. However, 'diet therapy' mostly restricts the diet excessively, and it is difficult to implement such therapy for a long time. Regarding the method of ingesting fat substitutes, although various fat substitutes are currently being developed, none of the fat substitutes is satisfactory (especially in terms of taste), so this method is quite difficult to implement. Known as appetite suppressants used in drug therapy are mazindol (Ciba-Geigy-NOVARTIS, trade name: "Sanorex ™"), fenfluramine (trade name) : "Pondimin ™", Wyeth-Ayerst Laboratories), etc. However, these drugs can only be used under the guidance of a physician. In addition, these drugs have been reported to have multiple side effects. Therefore, this type of drug therapy is associated with danger (see Non-Patent Document 1). Exercise therapy is the person who consumes calories through excess exercise. This method can be used in combination with diet therapy to obtain good results. However, it must be sustained for a long time under a strict management plan, and implementation is still accompanied by difficulties. As stated by uranium, the methods used to date as countermeasures for obesity have been difficult for patients to implement. In order to replace the obesity countermeasures mentioned above, currently researching and developing new functional preparations and even food and beverages, even when ingesting excess fat, it can also achieve the function of preventing fat from accumulating in the body (preventing the absorption of 20 fats) Effect or promote fat excretion). Typical examples of functional preparations or foods and drinks found through such research and development include products using chitosan. Chitosan used for this product has the effect of inhibiting fat accumulation, and the fact that this product can effectively act as a fat accumulation inhibitor has been redundantly described in Non-Patent Documents 2 and 3, Patent Documents 200533300 1-4, and the like. In addition, there have been reports that chitosan and Glucomannan can be used to reduce cholesterol (see Non-Patent Document 4). [Non-Patent Literature 1] Hadler · A · J: J · Clin. Pharm ·, 12, 453 (1972); 5 Stunkar. DA? Et al.? Lancet, 1, 503 (1973). [Non-Patent Literature 2 】 Nagyvary, J, J .: Nutr. Rep_ Int., 20, 677 (1977). [Non-Patent Document 3] I · Ikeda: J. Nutr ·, 119,1383 (1989). [Non-Patent Document 4] Cynthia M. Gallaher, et al., &Quot; Cholesterol Reduction by Glucomannan and Chitosan Is Mediated by Changes in Cholesterol 10 Absorption and Bile Acid and Fat Excretion in Rats, ff Journal of Nutrition 130: 2753-2759 (2000). [Patent Document 1 ] JP 6-7117 [Patent Document 2] JP 1M47828 [Patent Document 3] JP 200M69753 15 [Patent Document 4] JP 2003_113089 However, the functional preparations contained in these documents Neither the food nor the food or drink has yet to achieve a sufficient effect in practice. The related industry still hopes to develop a functional preparation or food and drink that has a better effect of inhibiting fat accumulation or inhibiting fat absorption. 20 [Belonging to dissolving in the month 1] Disclosure of the invention The problem to be solved by the invention Therefore, the purpose of the present invention is to provide a functional preparation and food and drink which use chitosan, which can achieve better results than the conventional ones Fat 200533300 Compositions (functional preparations and food and beverages) that inhibit accumulation (fat absorption). It is another object of the present invention to provide a method for inhibiting dietary fat absorption by administering or ingesting the composition. 5 Means to Solve the Problem The inventor of this case has repeatedly studied carefully in order to solve the foregoing problems. As a result, it has been found that in a composition composed of chitosan and Guanhua bean gum, pectin, jacaranda, or locust bean gum, several Butan has a significantly increased fat accumulation inhibiting effect, so it can provide a composition (functional preparations and food and drink) that can improve the absorption of dietary fat in accordance with the aforementioned purpose and has been improved. That is, it was found that if the composition is taken orally, the absorption of dietary fat can be significantly hindered. The present invention is based on such insights, and iteratively researched and completed them. The present invention can provide the composition for blocking dietary fat as listed in items 1 to 8 below. Item 1 A composition for hindering the absorption of dietary fat, comprising: (1) chitosan; and (2) a water-soluble dietary fiber selected from the group consisting of Guar Gum, pectin ( pectin), Tara viscera, and Locust Bean Gum. 20 帛 2 The composition for hindering the absorption of dietary fat according to item 1, wherein the water-soluble dietary fiber is at least one selected from the group consisting of guanhua bean gum, pectin, and locust bean gum . Item 3 is the composition for hindering the absorption of dietary fat according to item 1, wherein the water-soluble dietary fiber is Guanhua bean gum. 200533300 The fourth member, as described in item 1, is used to hinder dietary fat. It is 1 part by weight with respect to (1) chitosan contained, and it is a product containing water-soluble dietary fiber, and the water-soluble Sexual food ratio: ι Chinese bean gum, pectin, jacaranda, and locust bean gum 10 15 The 5th member of the 1st item is used to prevent the fat from being eaten by fat. Parts by weight are based on ^ weight ^ product 'contains (2) water-soluble dietary fiber' and the water-soluble dietary fiber:;: the ratio of at least huahua gum, pectin, and locust bean gum is at least ^ in The composition for blocking the absorption of fat by the 6th item and the 3rd item is contained in 3 to 1 part by weight of the chitosan 3 (1) (2) Guanhua bean gum. Item 7 The composition according to any one of Items 1 to 6 for preventing the absorption of fat from eating, which is in a form suitable for oral administration. The eighth member is a composition for hindering the absorption of dietary fat according to any one of items 1 to 6, which has a form of food and drink, and the form of the food and drink is selected from the group consisting of snacks, drinks, and supplements (suppi_nt). Group 0) In addition, the present invention can provide the methods listed in items 9-17 below to hinder the absorption of dietary fat 20. Item 9-A method for inhibiting dietary fat absorption in a person who needs to block dietary fat absorption, is to make the human body orally ingest an effective amount of the composition of any one of items 1 to 8. Item 10-A method for making dietary fat excretion in a human body that requires dietary fat to be excreted 200533300 The method for causing the human body to orally ingest an effective amount of the composition of any one of items 1 to 8. Item 11 is an anti-obesity method for a human body in need of anti-obesity, which is a method in which the human body orally ingests an effective amount of the composition of any one of Items 1 to 8. 5 Item 12: A method for inhibiting an increase in serum triglyceride after diet in a human body with a large amount of fat intake, which is a method in which the human body orally ingests an effective amount of the composition of any one of Items 1-8 . Item 13: The use of the composition of any one of items 1 to 8 in which the composition is used to impede the absorption of dietary fat. 10 Item 14 A use of the composition according to any one of items 1 to 8 for use of the composition to excrete dietary fat from the body. Item 15 A use of the composition of any one of items 1 to 8 to use the composition for anti-obesity. Item 16 The use of a composition according to any one of Items 1-8, which is used to suppress an increase in serum triglyceride after diet. Item 17: The use of (1) chitosan and (2) water-soluble dietary fiber, which are used to produce the composition of any one of Items 1 to 8, wherein the water-soluble dietary fiber is At least one selected from the group consisting of guanhua bean gum, pectin, thorn cloud gum, and locust bean gum. 20 The composition of the present invention can achieve a very excellent fat absorption blocking effect. This fat absorption inhibiting effect is more excellent than when chitosan is used alone. That is, the present invention has been found to be completed by a combination of chitosan and the aforementioned specific water-soluble food fiber that exerts a synergistic effect on the effect of inhibiting fat absorption. 10 200533300 In the present invention, the water-soluble dietary fiber that can be used in combination with chitosan is selected from the group consisting of guanhua bean gum, pectin, jacaranda gum, and locust bean gum. Of these, (a) is preferably at least one selected from the group consisting of guanhua bean gum, pectin, and locust bean gum. A particularly suitable water-soluble dietary fiber for use with chitosan is (b) Guanhua bean gum. In the composition of the present invention, each component used as an active ingredient is known in the food field as a food material, a food additive, and the like, and it is easily available. For example, chitin obtained by applying crustacean crustaceans, such as crabs and shrimps, to acid treatment, that is, as much as N-acetamido_D_10 glucosamine and D-glucosamine combined Chitin polymerase can be obtained as a component (1) by subjecting the saccharides to alkali treatment. The chitosan is also a non-bathable dietary fiber containing polyglucosamine as a component, which can be prepared according to a predetermined method. If you want to make it easier, you can get it by business methods. The content of the aforementioned (1) component in the composition of the present invention is not particularly limited as long as it can reach the cost of the fruit of the invention. Usually, the composition contains 0.1% by weight or more, and preferably contains 2 to 80% by weight, and more preferably contains 2 to 50% by weight. In the present invention, 'the material sentence τ constituting the (2) component which is used in combination with the aforementioned (1) component belongs to the water / cereal dietary fiber (20SDF)' in general 'in the food field It is used as a food and beverage additive (thickening stabilizer). The ° Kuanhua bean gum of sheep's mouth is made from the seeds of Guanhua bean tree of the leguminous family, which is still extracted from the germ. It is produced mainly from polysaccharides combining glucose and mannose. Ingredients (about 78 ~ 82% galacid 11 200533300 mannan). The Guanhua bean gum of the present invention also includes Guanhua obtained by decomposing and processing the Guanhua bean gum with enzymes (such as hemicellulase, galactomaimanase, etc.). Degradation of glutinase. Pectin is a kind of polysaccharides that form the skeleton of plant tissues. 5 It can be prepared from various plants. It consists essentially of a conjugate of galactouronicacid which has undergone amidation. Pectin can be broadly divided into water-soluble pectin ester acids; insoluble protopectin that combines with other polysaccharides, proteins, and inorganic substances; and pectin acid that can decompose the original pectin. The invention can use any of these. The degree of methyl esterification of methylated galacturonic acid constituting pectin 10 is known to be about 0 to 80%, and any degree of fluorenyl esterification may be used in the present invention. Citrus aurantium gum is a seed polysaccharide prepared from the germ of a seed of the kiwi family, Acanthaceae, α cd / osa Z ·). Locust bean gum is a polysaccharide (containing about 88% galactomannan) prepared from the germ extract 15 of the seeds of Robinia pseudoacacia (⑽). These substances can be prepared according to the law from various plants containing them as described above, and can be obtained by commercial methods for simplicity. (2) of these: One kind may be used alone or two or more kinds may be combined, and then used in combination with the chitosan of the aforementioned (丨) component. 2 0 | The amount of the aforementioned component (2) contained in the composition for hindering the absorption of dietary fat of the present invention is not particularly limited before it reaches the effect of the invention. Generally, its blending amount is 1 part by weight with respect to the aforementioned (1) knife included in the composition of the present invention, and the total amount of Guanhua bean gum, pectin, thorn cloud gum, and locust bean gum is in the range of parts by weight of Mo. And, it is preferably in the range of 3-7 parts by weight, and more preferably in an amount of about 5 12 200533300 parts by weight. By using the above-mentioned (1) component in combination with the above-mentioned (2) component, especially in the above-mentioned specific mixing ratio, the (1) component and the (2) component in the obtained composition will multiply each other , And can exert an excellent effect of preventing fat absorption. In addition, in the composition of the present invention, the (1) component and (2) component, which are effective ingredients, have been used in Japan as food and food additives, and safety has also been confirmed as a material that can be used continuously. Therefore, the composition of the present invention for preventing the absorption of dietary fat, which is a combination of these combinations, is orally ingested (administered) is harmless and can be used continuously. The focus of the composition for hindering the absorption of dietary fat of the present invention is to contain the aforementioned (1) ingredient and (2) ingredient as essential ingredients, but it is not necessary to consist of only these ingredients, without impairing the original diet of the composition of the present invention Under the premise of obstructing fat absorption and other effects, in order to prepare the composition into a form that can be easily ingested or administered, it can contain edible ingredients that are acceptable for food hygiene or ingredients that are acceptable for formulation. Comes as an auxiliary ingredient. In addition to the aforementioned components (1) and (2), in the composition of the present invention, the food is hygienically acceptable edible ingredients that can be included as supplementary ingredients as needed, in addition to the three major nutrients such as sugars, fats and proteins. In addition, it also contains 20 food ingredients and food additives that are generally known for use in preparing food and beverage. More specifically, the food materials and food additives include: sweeteners such as natural sweeteners (other than sugars) and synthetic sweeteners; vitamins and minerals (electrolytes and trace elements); natural flavors and synthetic flavors Other flavoring agents; coloring agents; preservatives; natural fruit juices, natural pulp, etc. 13 200533300 In addition, the sword-making acceptable ingredients of the aforementioned auxiliary ingredients include preparations known to be used in the preparation of medicaments for administration, such as occupational agents (fillers), 1 agent, Binding agent, moisturizing agent, disintegrating agent, smoothing agent, extender and surfactant. The preparation can be prepared according to the desired dosage unit form and 5 is appropriately selected from these. The composition of the present invention can be prepared into an oral composition in accordance with a predetermined method by using the aforementioned component (1), the peptone component, and as needed, and assisting in forming a knife. Oral-grade products Daggera Oral medicines, medicines, and food and beverages. In addition, it is preferable that the diet 10 15 20 Mouth Mouth Mouth Mouth include functional foods and foods for specific health care which have an effect of hindering the absorption of dietary fat. The composition of this Maoming that prevents the absorption of dietary fat can take the form of a drug or a quasi-drug as long as it can be used orally, and is subject to special restrictions. This form includes, for example, pills, powders, suspensions, emulsions, granules, capsules, gums, syrups and the like. The modulation method for preparing these forms can be performed according to the general medical method. "X shellfish, please take your mouth" "==: Drinks that have a composition that interferes with the absorption of dietary fat = sad and not continent _ system, such as: (A) milk drink sleepy drinks, refreshing drinks, carbolic acid drinks, fruit juice drinks , Vegetable drinks, vegetable and fruit juice drinks, green drinks, alcoholic beverages, brown drinks, black tea drinks, green tea, mixed tea, fancheng materials, beverage forms such as beverages, Gaya, etc. ) Puddings, puddings, fruit puddings, puddings, fruit lings, milk shreds, yogurt, etc .; (C) ice cream, milk ice, Lact0-ice, Hungarian ice, popsicles, etc .; soft Sugar (including 14 200533300 containing milk candy, nougat, QQ sugar, marshmallow, etc.) and hard fruit (including toffee, etc.) and other confectionery; (E) hard biscuit, cookies , Crackers, persimmon mochi, pancakes, coriander sticks, burdock sticks, and other snacks, as well as puff pastry products using an extruder, etc. (F) Bread, vegetable bread Bread form; (G) cormorant form, etc .; (H) soup, juice, etc. State; stuffed products such as bonito board, sausage, etc .; (J) food form of conditioning package such as curry;面, spaghetti and macaroni noodles; other supplements (complementary foods) in the form of pills, tablets, 10 tablets, granules, powders, freezers, capsules, etc .; (M) pure fluid food, high Nutrient-rich liquid foods such as oral liquid foods, etc. Among them, the preferred food and drink products include (A) beverage forms, (E) snack forms, and (L) supplements. The composition for hindering the absorption of 15 fats of dietary fat of the present invention is not only suitable to mix the components (1) and (2) in the aforementioned proportions, but also can be used conventionally in preparation according to the form of the desired food and drink and the manufacturing method thereof. In addition, when the composition for hindering the absorption of dietary fat of the present invention is prepared into a liquid form such as a beverage form, although it is not particularly limited, it should preferably be adjusted to a viscosity of 1 to 3000 mpa · s (compared to 20). Preferably 10 ~ 100mpa · s, And more preferably within the range of 50 ~ 500mPa · s) (Conditions for measuring viscosity: use

Brookfield viscometer, 30 rpm, 20 ± 0.5. 〇. The dosage of the composition for hindering the absorption of dietary fat according to the present invention may depend on the form of the composition (dietary product form or pharmaceutical form), and the patient who wants to administer it. The degree is determined as appropriate, and there is no particular limitation on the amount of g, and the amount of the ingredient (1) in the range of 0.1 to 5 g can be used as a standard for one adult and one person, so that a sufficient effect can be obtained. The composition for blocking the absorption of dietary fat of the present invention can be taken through it; the absorption of dietary fat in the tongue can be prevented, and obesity can be prevented without restricting diet and medication, and circulatory diseases, respiratory diseases, diabetes, etc. The onset of various comorbidities. Therefore, the present invention can provide a method for obstructing the absorption of dietary fat in a person who needs to block the absorption of dietary fat, which is to make the human body orally ingest 10 effective amounts of the composition of the present invention. In addition, the present invention can provide a method for excretion of dietary fat in a human body which needs to eat fat and excrete the body, which is to enable the human body to orally ingest an effective amount of the composition of the present invention. 15 20 Furthermore, the present invention also provides an anti-obesity method for a human body in need of anti-obesity, which enables the human body to finely ingest an effective amount of the composition of the present invention. In addition, the present invention can also provide a method for inhibiting the right to drink serum triglyceride in a human body with a large amount of fat intake, which can effectively ingest an effective amount of the composition of the present invention by the human body. In the aforementioned method of the present invention, the composition of the present invention requires a human body to shoot, turn into, and cut into a given period of time and meal time (photographed by the fat # to the group of the present invention, "can be used as a poem, However, if the effect of I & moon fat absorption is considered, it is advisable to administer the dietary fat absorption knife at the same time as the meal or before or after the meal. 16 200533300 The composition of the present invention can exert effects on the human body. In addition, the present invention can also provide the following uses, that is, the use of the composition of the present invention to prevent the absorption of dietary fat; the composition of the present invention is used to make the dietary fat Use for excretion; use of the composition of the present invention for anti-obesity; use of the composition of the present invention for inhibiting the rise of serum triglyceride after diet. In addition, the present invention also provides (1) chitin The use of polysaccharides and (2) water-soluble dietary fiber are those used to manufacture the composition of any one of item 8, wherein the water-soluble dietary fiber is selected from the group consisting of Guanhua bean gum, pectin, Thorn cloud glue and thorn Shu at least the species of the group consisting of bean gum.

In particular, according to the method of using the composition of the present invention to inhibit the absorption of dietary fat, it is possible to suppress an increase in serum triglyceride after a meal. Serum triglycerides rise after meals, especially as dietary fat intake increases, so does the tendency to rise. The post-meal serum triglyceride rises to an abnormally elevated state or a state in which this elevated state is prolonged is called Postprandial hyperlipidemia (by Tadano Kifu, Mobil ^ '27 (12), 319-325 (2000)). In recent years, it has been reported that Z kinds of postprandial dyslipidemia are independent risk factors for coronary artery disease (refer to: Tada Yufu, Yuri ^, 27 (12), 319-325 (2000): Patsch, JR., et al "Areterioscler. Thromb., 12, 1336-1345 (1992), etc.) According to the method of using the composition of the present invention to inhibit the absorption of dietary fat, it is possible to significantly inhibit (block) the fat absorption from the diet. This also means Can inhibit the rise of serum triglyceride after 4. Therefore, the composition of the present invention can reduce the risk of postprandial hyperlipidemia and the risk of green artery disease by 2005. As mentioned above, the present invention can also Provided is a method for treating postprandial hyperlipidemia and a method for preventing coronary artery disease. In addition, the present invention also includes the following aspects. A · A method for enhancing the effect of inhibiting dietary fat absorption by chitosan, which is characterized by : Use (丨) chitosan and (2) at least one of the components selected from the group consisting of Guanhua bean gum, pectin, ganoderma gum, and locust bean gum. B. — Chitin With Guanhua bean gum, pectin, Thorn cloud glue or thorn

A method for mutual enhancement of the effect of locust bean gum on inhibiting the absorption of dietary fat, characterized in that (1) chitosan and (2) are selected from the group consisting of Guanhua bean gum, pectin, ganoderma 10 and gum locust bean At least one of the ingredients of the gum is used in combination. c. The method for enhancing the effect of inhibiting dietary fat absorption of chitosan as described in a. above, which is selected from Guanhuadou (2) based on the total weight percentage of WO with respect to the total weight of chitin. A combination of at least one component of gum, pectin, muscari, and locust bean gum is used in combination. 15 d. As described in b. Above, Chitosan and Guanhua bean gum, pectin, thorn cloud fruit: or Robinia pseudoacacia to prevent the secret food ㈣ "the method of transmutation, ', will be relative to ⑴Chitin The total amount of 1 part by weight of glycan is (2) selected from the group consisting of guanhua bean gum and glutamate. The top 20 methods of Secret, Sophora acuminata and Locust Bean Gum, which are used to make dietary fat absorption obstructive. 0 e · As in any one of the foregoing 1 to d ·, the multiplication of the obstruction of fat absorption is multiplied. The effect of the formula with enhanced sexual properties is to combine at least one ingredient selected from the group consisting of 18 200533300 gum, pectin, cuspidaceae, and locust bean gum by the composition of the present invention of Guan Huadou. This significantly enhances the effect of the component (1) that hinders the absorption of dietary fat, and as a result, it can exert a very excellent effect that hinders the absorption of dietary fat. Therefore, the composition of the present invention can be prepared as a dietary fat absorption inhibiting agent, an anti-obesity agent, etc., and is particularly useful in the medical field. In addition, in the food field, it can be prepared as a food and drink product, which has the ability The function of fat is not absorbed by the body and is effectively excreted from the body, so it is very useful, especially as a functional food (specific health food). Simple explanation of formula 10 Figure 1 is a graph showing the test results of Experimental Example 3 (horizontal axis: time after giving (hours), vertical axis: plasma triglyceride concentration (mg / dL)). Figure 2 is a bar graph showing the test results of Experimental Example 4 (vertical axis: liver triglyceride amount (mg / g liver). Figure 3 is a graph showing the laboratory according to Experimental Example 5 Result of bleeding 15 triglyceride concentration (horizontal axis: time (time) after ingestion of fatty food, vertical axis: serum triglyceride concentration (mg / dL)). [Embodiment The best embodiment of the present invention Experimental examples and examples are listed below to explain the present invention, but the present invention is not limited by these experimental examples, etc. In addition, in the following experimental examples and the like, unless otherwise specified, the "%" of the blending ratio is weight %, “Parts” are parts by weight. F Test Example 1 In order to evaluate the effect of the composition of the present invention on obstructing the absorption of dietary fat, 19 200533300 The following test was performed using rats as test animals. Effects of ingestion of a chitin poly _ and feeds of Tongguanhua bean gum or pectin on fat excretion in feces. 1. Test animals and breeding f 5 Test animals used male SD Rat (6 weeks old, weight: 192 ~ 208g) (purchased from: CL⑷apanjne) 32_ · 4 only). These animals were individually housed in stainless steel cages of 22Wx 29Wx 21H during the H-test period. The temperature was 22 ~ 24 ° c, the humidity was 51 ~ 60%, and the lighting was U hours (7-9pm) / day. Breeding. Feed until the beginning of the experiment (mixed bait was started at 10) was given standard rat feed (AIN93G compound feed, powder, 〇riemal heart c0, ud), and water was freely taken from tap water.

15 20 < Rat standard feed composition (unit: g) > Casein 200 α-Corn Starch 132 β-Corn Starch 397.5 Vegetable Sugar 102.5 Soybean Oil 70 ΑΙΝ93 with mixed minerals 35 ΑΙΝ93 with mixed vitamins 10 L-half Glutamate 3 butyl hydrochloride 0.014 Cellulose 50 Total 1000.014 20 200533300 In addition, AIN93 with mixed minerals and AIN93 with mixed vitamins both use 〇HemalYeastc〇.

The feed of each group of rats was replaced with each of the following mixed bait feeds and the test was started. They were fed with mixed feed for 6 days. In addition, rats in the control group continued to receive standard diets. Test group 1: Chitosan 1% + Guanhua bean gum 5% mixed bait feed: change corn starch 3 9 7 · 5 g of standard feed to 3 3 7 .5 g, and add chitosan l〇 g and Guanhua bean gum 50g feed 10 Experimental group 2: Chitosan 1% + pectin 5% mixed bait feed: Change the standard feed corn 丨 dian powder 397.5g to 337.5g, and add chitosan 10 g and 50 g of pectin feed. Comparative group 1: Chitosan 1% mixed bait feed: 397.5g of corn diet powder of standard feed was changed to 387.5g, and 10g of chitosan was added to the feed. 15 Comparative group 2: Guanhua bean gum 5% mixed bait feed: 397.5g of corn starch of standard feed was changed to 347.5g, and 50g of Guanhua bean gum was added. Comparative group 3 · · Pectin 5% mixed bait feed: 397.5 g of corn starch as standard feed was changed to 347.5 g, and 50 g of pectin was added as feed. In addition, as the chitosan, a product made by Koyo Chemical Co., Ltd. (Koyo 20 Chemical Company Limited, Lot No. 0817-13 'viscosity: 105 mPa · s, deacetylation degree: 89.1%) was used. Guanhua bean gum uses "BIS-TOP TM" manufactured by San-Ei Gen F.F.I., Inc. As the pectin, "SM666" (trade name) manufactured by San-Ei Gen F.F.I., Inc. was used. 21 200533300 3. Measurements and measurement methods From the beginning of the experiment to the 4th to 6th days, filter paper was spread under the rearing cages of each group of rats, and all the feces discharged thereon were recovered. Feces are recovered 丨 times daily, between 9: 00-10: 00 daily. After measuring the wet weight of feces, it was freeze-dried (5 days). The dried feces uniformly pulverized by a stirrer were prepared as samples for measuring the fat content in the feces. Fat content in feces was measured by the method of Folch (J. Biol. Chem., 226, 499 (1957)). Total fat excretion is calculated by the following formula 1. The external fat absorption rate was calculated by the following formula 2. Formula 1: Total fat excretion amount = AXB 1〇A: Fat content in feces B: Wet weight of dry feces Formula 2 · Appearance fat absorption rate (x ^ ioo ^ c / Dx 100) C ·· 1 day of feces Total fat excretion D: The average fat intake on the 1st day (average food intake X 7%) < 1 / 1〇) 15 The results obtained are expressed as the mean ± 95% confidence interval value. The multiplicative test is performed using 1-Way ANOVA (ADDITIVE EFFECT) of statistical analysis software 88.8 Versión 8 (manufactured by SAS Institute). 4 • Measurement results The results are shown in Table 1 below. 22 200533300 Table 1 Total fat excretion in group (mg / 3) Apparent fat absorption rate (%, experimental group 1 3236+ 691 * 19.1 ± 8.7 * experimental group 2 2085+ 750 # 51.0+ 14.8 # comparative group 1 428 + 533 91.6+ 9.3 * Comparative group 2 598+ 180 84.7+ 5.2 · —Comparative group 3 637+ 70 84.6 ± 0.8 'Control group 140+ 38 97.0 ± 0.8 From the results shown in Table 1, the following can be confirmed. Compared with the control group In comparison, the total fat excretion of comparative group 1, comparative group 2, and comparative group 3 increased. In contrast, experimental group 1 using chitosan and Guanhua bean gum and using chitosan and 5 pectin The total fat excretion of experimental group 2 increased significantly compared with that when chitosan (comparative group 1), Guanhua bean gum (comparative group 2), and pectin (comparative group 3) were used alone. This effect is seen through Statistical analysis shows the effect of multiplication (in the table, marked with ★ and #). In addition, in terms of appearance fat absorption rate, experimental group 1 and experimental group 2 used chitosan alone Π) Guanhua bean gum (comparative group 2) and pectin (comparative group 3) also decreased frequently compared with the lower ones. This-effect is displayed as a multiplying reduction effect through statistical analysis (in the table, it is marked with ★ and #). Experimental Example 2 15 The composition of the present invention inhibited the absorption of dietary fat, and the following experiments were performed. This experiment uses experimental animals of the same age as the experimental example, and then «Squamble Field Ingestion Group ⑺ Locust Bean Bean _Materials« Summary == and the effects caused by ^ 异 1 ^ day fat excretion

23 200533300 Animals tested were male SD rats (8 weeks old, weight: 312 ~ 365g) (speaking green: CLEA JaPan, Inc.) 24 (6 groups X 4). These test animals were tested, and the sacral system was housed in a 2-ch 29Wx 21H (cm) stainless steel compound, and the temperature was 22 ~ 24 ° C, humidity was 51 ~ 60%, and lighting was performed for 12 hours. Feeding. Feed until the beginning of the experiment (beginning of the mixed bait) was given an atmospheric standard feed (AIN93G compound feed, powder, 〇rientd valence c0 ·, toe) with the following composition, and water was free to take tap water. 2. Experimental method and experiment $ Replace the feed of each group of rats with the following mixed bait feeds and start the experiment. Each mixed bait was fed for 6 days. In addition, rats in the control group continued to receive standard diets. Experimental group 3 · Chitosan 1 ° /. + Locust bean gum 5% mixed bait feed: 397.5g of corn starch for standard feed was changed to 337.5g, and 10g of chitosan and 50g of locust bean gum were added.

Comparative group 4: Chitosan 1% mixed bait feed: 397.5g of corn starch of standard feed was changed to 387.5g, and 10g of chitosan was added to the feed. Comparative group 5: locust bean gum 5% mixed bait feed: 397.5 g of standard meal powder was changed to 347.5 g, and 50 g of locust ugly gum was added. 20 In addition, the chitosan line uses Jiayang Chemical Co., Ltd.

Chemical Company Limited, Lot No. 0817-13, zero occupancy 105 mPa · s, degree of deacetylation: 89.1%). Locust bean gum is 隹 ^ ° y〇 degrees "BIS-TOP ™ D-30" manufactured by San-Ei Gen F.F.I., Inc. Rongpu 24 200533300 3. Measurement items and determination of tadpoles The same as in Experimental Example 1, the whole amount of feces was recovered for 3 days and the weight was measured on the 4th to 6th days of the experiment, and the content of fat in the recovered feces was measured. The total fat excretion amount and apparent fat absorption rate were calculated in the same manner as in Experimental Example 1. 5 Results are expressed as mean ± 95% confidence interval values. The multiplicative test is performed by the 1-Way ANOVA (ADDmVE EFFECT) method using statistical analysis software SAS Version 8 (manufactured by SAS Institute). 4. Measurement results The results are shown in Table 2 below. 10 Table 2 Group total fat excretion (mg / 3 days) Apparent fat absorption rate (%) Test group 3 1 084 ± bucketed * 78.3 ± 6 furnace comparison group 4 274 + 74 94.9 ± 1.1 comparison group 5 516+ 227 89.5 ± 3.7 Control group 237+ 23 96.0+ 0.4 From the results shown in Table 2, the following matters can be confirmed. Compared with the control group, even when chitosan (comparative group 4) and locust bean gum (comparative group 5) were used alone, the total fat excretion increased. Compared with these conditions (Comparative groups 4 and 15 5), the experimental group 3 using locust bean gum combined with locust bean gum showed a more significant increase in total fat excretion. The effect seen in this experimental group 3 is a statistical multiplication increase effect (in the table, marked with a *). In addition, the appearance of fat absorption rate was significantly lower than that of chitosan alone (comparative group sentence and locust bean gum (comparative group 5)) in experimental group 3. This effect was shown by 20 statistical analysis, as Multiplication reduction effect (indicated by the mark * in the table). From the above facts, it can be seen that if the combination of (1) component and (2) component 25 200533300 of the present invention is combined, one person can achieve significant dietary fat absorption 丨And the effect is hindered. Among them, the combination of chitosan and Guanhua bean gum (Experiment 2)

(Call) to achieve a particularly strong effect. As mentioned above, the combination of the components (1) and (B), A, J, and Sabre can increase the fat excretion by multiplication. 5 Experimental Example 3 I The following tests were performed to evaluate the effect of the composition of the present invention on the effect of inhibiting the fat absorption of food. In this experiment, rats were used as the test animals, and the effects of ingestion of a diet combined with chitin chitin and Tongguanhua bean gum on plasma triglyceride concentrations were examined. 10 1. Test animals and breeding strips # The test animals used male SD rats (9 weeks old, weight: 307 ~ 342g) (purchased from: CLEA Japan, Inc.) 22 (6 groups X 4). The test animals were individually housed in stainless steel cages of 22Wx 29Wx 21H (cm) during the test period, with a temperature of 22 to 24 ° C, a humidity of 51 to 60%, and 12 hours of lighting for 12 hours (7 o'clock to 9 o'clock). / Day conditions. Water is free to tap water. kExperimental methods and freshwater feeding Male SD rats were fed a 20% fat-refined feed of the following composition for 1 week. The feed is taken freely from 10: 00 to 10: 30 and 12:00 to 17:00 20 times daily. After 1 week, the rats were divided into the present invention group and the control group (the present invention group was 10 and the control group was 12) having the same average weight. Rats of each group were fasted for a day from 10:30, and from 10:00 to 10:30 the next day, chitosan lg / 100g and Guanhua bean gum 3g / 100g were added instead. 4g / 100g of cellulose in a refined feed containing 20% fat 26 200533300 (Feed of the present invention) 4g was fed to each rat of the present group. Rats in the control group were given 4 g of refined feed containing 20% fat. < Refined feed with 20% fat (unit: g) > 10 Casein α-Corn Starch / 3-Corn Starch Sucrose Soybean Oil Mixed Minerals ΑΝΝ93 Mixed Vitamins ΑΙΝ93 L-cysteine butylhydroxyquinone 200 132 277.5 102.5 200 35 10 3 0.014 Total cellulose 15 20 40 1000.014 In addition, ΑΝΝ93 of mixed minerals and 取 93 of mixed vitamins were both manufactured by Oriental Yeast Co., ltd. 3. Measurement items and measurement methods In the feed of the present invention or refined feed containing 20% fat, 彳-込 ^ Blood is collected from ear leads and veins and separated from blood plasma at various periods of 1, 2, 3, 4 and 6 hours after the start of feeding. , And then use commercially available test rats Takamaki-san, Takilyceride E-Test WakoTM, and $ Chemicals Pure Chemical Industries, Ltd.) to determine Amount (mg / dL) ° Determination of glycerol and glycerol in blood after a predetermined period of time has elapsed since the feeding of the bait. 27 200533300 Subtract the measured value before feeding the bait. , Mg / dL). The results obtained are expressed as mean ± standard error. The comparison between the group of the present invention and the control group is carried out with a non-corresponding 1 test (SAS Version 8). The intentional level is 5 on both sides %, And the hazard rate was judged as having an error rate of 5 5.0% or less. 4. The measurement results are shown in Figure 1 (horizontal axis ·· time after feeding (hours), vertical axis: plasma triacid The amount of glycerolipid change (increased amount) (mg / dL). In the figure, (1) represents the present invention group As a result, (2) represents the results of the control group. The asterisks giving the group values of the present invention after _2 hours and 3 hours indicate lower values that are meaningful relative to the control group (p < 0.05). It is clear from the results shown in Figure 1 that the plasma triglyceride content of the present invention group is significantly lower than the control group. Based on this, it can be seen that ingestion of the composition of the present invention can hinder dietary fat absorption. 15 Experiments Example 4 • To evaluate the effect of the composition of the present invention on inhibiting the absorption of dietary fat, the following experiment was performed. In this experiment, rats were continuously ingested the composition of the present invention, and the continuous intake was used to investigate the amount of fat excretion in fecal atmosphere. , Adipose tissue weight and liver triglyceride content change over time. 20 k test animals and feeding blind chromium duo test animals used male SD rats (5 weeks old, weight: 137 ~ 155§) (purchased from : (: 1 ^ eight; milk, 111 (:) 31 (8 > < 3 groups + 7 X 1 group). The feeding conditions of these tested animals are different from the type of mixed bait feed used. Experimental example 3 is the same. 28 200533300 LExperiment method and 眚 brig # Feed system under the use Each mixed bait feed was bred for 28 days (inventive group, comparative groups 1 and 2). In addition, the control group of rats (n = 8) was given a refined feed containing 20% fat containing the composition contained in Experimental Example 3. 5 Inventive group ㈣): Chitosan original Guanhua bean gum 3% mixed feed: Feeding: In the refined feed containing 20% fat as shown in Experimental Example 3, 40g / 1000g of cellulose was replaced with chitosan1. g / 1000g and Guanhua bean gum 30g / 1000g. Comparative group 1 (n = 7): Breeding with chitosan 1% mixed bait feed: In experiment 10, the composition shown in Example 3 contained 20 ° /. In the fat-refined feed, 10 g of 40 g of cellulose was replaced with chitin 10 g. Comparative group 2 (n = 8): fed with Guanhua soybean gum 3% mixed bait feed: In a refined feed containing 20% fat containing the composition shown in Experimental Example 3, 30 g of 40 g of cellulose was replaced with Guan Hua 30 g of soy gum. 15 Determination of the order and measurement method Within 3 days from the 24th to the 27th day after breeding with mixed bait feed, the feces of each group of rats were recovered in the same manner as in Experimental Example 1, and the total fat content in the feces was measured (g / 3 days) ). In addition, the triglyceride content (mg / 3 days) and free fatty acid content (μΕς / 3) of feces were measured simultaneously. 20 Triglyceride content in feces was determined by the Fletcher method (C7h. C / zim. 22, 393, 1968). The Folch extract (Folch extract) obtained by the Folch method shown in Experimental Example 1 was dried, and the obtained residue was made into a sample, and then a commercially available measurement kit (NEFA C-Test and Light ™ (NEFA C- test wako (TM); Wako Pure Chemical Industries, Ltd.) measured 29 200533300 free fatty acid content in stool. In addition, "the body weight of each group of rats was measured by electronic celestial measurement before the start of the breeding period and the end of the breeding period" to calculate the weight gain (Ag). During the test period, the amount of rats in each group was measured every day, and the total amount was calculated (total volume 5 g). The daily feed intake is a value obtained by subtracting the amount of feed remaining on that day from the daily feed amount given to each group of rats. From the weight gain ⑻ and total tilt (F) obtained as described above, the lexical efficiency ⑼ (feed) is calculated according to the following formula. Food efficiency (g / 100g feed) = E + Fx 100 more than Wei group after the end of the breeding period (28 days), the amount of liver weight π) and the weight of adipose tissue around the kidney (g), Calculate the weight of each viscera per 100 g of body weight (g / _body weight). In addition, after taking fat according to the method of Foleh et al., "One day in the liver (g)" was measured by the Fletcher method (Clin. Chim. Acta, 22, 1968), and triglycerides per gram of liver were different. Fat (g / g liver). 15 Secret results are expressed as mean ± SD. The comparison between the groups was performed by the Tukey-Ker method (SAS Versión 8). The intentional level is 5% on both sides, and the hazard rate below 5.0% is considered to be intentional. 4 • Measurement results The results are shown in Tables 3, 4 and 2 below. The English letters in the upper right of the numerical values shown in Table I are used to indicate the presence or absence of intentional differences, and the different English letters indicate intentional differences (㈣05). For example, in terms of efficiency, the value b in the group of the present invention is compared with the value of the control group (the value of the group), and there is no intentional difference. In addition, this value and the value of the comparative group worker and the comparative group 2 (values of the marks a, b) did not show an intentional difference. Furthermore, the values of comparative group 1 and comparative group 2 compared with the values of control group than 30 200533300 showed no significant difference. Similarly, in the weight of adipose tissue around the kidney, the value of the group of the present invention (value of mark c) is compared with the value of comparison group i (value of mark b), the value of comparison group 2 (value of mark ab), and the value of the control group. Any one of the values (the value of the mark a) showed 5 meaning differences (both P < 0.05). In comparison group 1, the weight of the surrounding adipose tissue of the kidney is labeled b. As shown in the figure, there is no intentional difference compared with the value (ab) of comparative group 2, and the intentional difference is compared with the value (a) of the control group. The weight of the surrounding kidney tissue of the comparative group 2 is marked ab, and as shown, the comparison with the value (a) of the control group shows no intentional difference. 10 Table 3 Group measurement items The present invention group (n = 8) Comparative group 1 (n = 7) Comparative group 2 (n = 8) Control group (n = 8) Weight before the test (g) 146.5 ± 5.8 145.9 ± 5.9 146.9 ± 5.5 145 · 9 ± 5 · 9 Weight at the end of the test (g) 328.8 ± 36.4 344.9 ± 123 350.5 ± 12.4 355.2 ± 12.2. Weight gain (Ag) 182.3 ± 36.4 199.0 ± 11.0 203.6 ± 11.3 209.3 ± 9.7 Total photo 1 Ear volume (g) 494.7 ± 55.4 494.6 ± 10.2 509.3 ± 13.0 512.0 ± 12.4 Feed efficiency (g / 100g feed) 36.6 ± 4.4b 40.2 ± 1.8 @ 40.0 ± 2.0 ^ 40.9 ± 1.7a Liver weight (g / lO〇g Weight) 2.8 ± 0.2 3.0 ± 0.2 2.7 ± 0.1 2.8 ± 0.2 Weight of adipose tissue around the kidney (g / 100 g weight) 1.2 ± 0.4C 1.9 ± 0.2b 2.0 ± 0.4ab 2.4 ± 0.4a

Table 4 Group measurement items The present invention group (n = 8) Comparative group 1 (n = 7) Comparative group 2 (n = 8) Control group (n = 8) Total fat (g / 3 day) 1.18 ± 0.24c 0.62 ± 0.23b 0.24 ± 0.05a 0.20 ± 0.03a triglyceride (mg / 3 daytime) 15.4 ± 4.5C 10.6 soil 3.9b 4.2 ± 2.9b 4.2 ± 0.8a free fatty acid & Eq / 3 daytime) 1357 ± 434b 621 Taxi 203a 337 Soil 97a 317 Taxi 126a From the results shown in Table 3, Table 4 and Figure 2, the following matters can be confirmed. That is, the weight of adipose tissue around the kidneys of the present group showed a deliberately lower value than that of the comparative group, the comparative group 2, and the control group (see Table 3). Regarding the tris acid in the liver 31 200533300 ▲ σ The present invention group shows a relatively low value in comparison with the comparison group 1, the comparison group 2 and the control group (see Fig. 2). In addition, both the total fat excretion amount, triglyceride excretion amount, and free fatty acid excretion amount of feces: the present invention group and the comparison group 1, the comparison group 2 and the control group all showed a deliberately higher value than the comparison group 5 (Table 4⑹. From the above, the following conclusions can be drawn. According to the present invention, the combined intake of chitin and guanhua bean gum is compared with the individual intake conversion (comparison | parent group 丨 and comparative group 2). Exhibit significant obstructive effects on dietary fat absorption; and 10 (2) continuous intake of the composition of the present invention compared with the ingestion of chitin and Kuhua bean gum (Comparative Group 1 and Comparative Group 2) alone, body fat can be obtained Experiment 5 In order to evaluate the effect of the composition of the present invention on obstructing the absorption of dietary fat, the following experiment was performed. This experiment selected an adult male as the subject, and investigated that the 15 subjects ingested the composition of the present invention for serum triacid Effect of glycerol lipid value. 1. Subjects Subjects selected 8 adult males (normal healthy persons). None of these subjects had received any medical treatment including administration of hyperlipidemia therapeutic agents. 20 Subject age is 34 The age range is from 51 to 51 years (average age: 43 ± 2 years), the weight is in the range of 58.7_116.0 kg (average weight: 76 · 9 ± 6.3 kg), and the BMI [weight kg / (length m) 2] is 20.4- In the range of 38.5kg / m2 (average BMI: 36 · 0 to 1.9kg / m2), the serum triglyceride fat value is in the range of i0i_i81mg / D1 (average: 132 ± 10mg / dL), and the serum total cholesterol value is in i59 -294mg / dL range 32 200533300 (average: 224 ± 16mg / dL) 2. Experimental method Subjects ingest the following specified food before the 2nd day of the test, and go on a hunger strike for about 14 from the day before the test Hours. On the start day of the test, only 5 servings of fatty foods with the following composition were taken or 2g of the following test substance was taken orally at the same time as oral fat foods. The test was conducted by the Crossover method with an observation period of 1 week. Prescribed foods> Day 1: 10 Breakfast: Caloriemate Block (manufactured by Otsuka Pharmaceutical Co., Ltd.) 4 (400kcal) Lunch: Bento A Dinner: Bento B Day 2: 15 Breakfast: Caloriemate Block (manufactured by Otsuka Pharmaceutical Co., Ltd.) 4 Bar (400kcal) Lunch: Bento A Dinner: Casual Sushi < Fat Food > 20 Corn Cream 200g thick soup 19g lard 15g total calories 438kcal fat 40.4g 33 200533300 carbohydrate 14.8g protein 3.8g ash 2.1g < test substance > 5 granules of mixture of Guanhua bean gum and chitosan (guanhua bean Gum: Chitosan = 3: 1 weight ratio). The particles were prepared as follows. That is, a powder mixture consisting of 23 parts of chitosan for food, 4_1 parts of Shiya (produced by Showa Sangyo Co., Ltd., corn-derived protein) and 0.4 parts of medium-chain fatty acid triglyceride was carried out by a fluidized layer 10 granulation method. Production. 72.5 parts of Guanhua Bean Gum was mixed with an appropriate amount of water to prepare a material for granules. The material was extruded and granulated to produce granules, which were dried in a shed vacuum dryer, and then subcontracted to produce a test object. Before the start of the test (intake of fatty foods) and 1, 2, 3, 4 and 6 hours from the start of ingestion of fatty foods, blood was collected from the subjects, and the 15 serum triglyceride concentration was measured by the enzyme method, and the serum was plotted. Triglyceride concentration curve. The results obtained are expressed as mean ± standard deviation. Comparisons between groups were made with corresponding t-tests (SAS Version 8). The intentional level is 5% on both sides, and a hazard rate of 5.0% or less is considered intentional. 3. Measurement result 20 The results of the obtained serum triglyceride concentration are shown in Fig. 3 (vertical axis: serum triglyceride concentration (mg / dL), horizontal axis: time after ingesting fatty food ( hour)). In the figure, each curve indicates the following groups. 0: Control group (non-administered test subject group) 34 200533300 2: The present invention group (administered 2g test subject and fat food group at the same time) In Figure 3, 3 hours after ingestion of fat food, this group The birth number of the invention group (2) indicates that the serum triglyceride concentration value of this group showed a significant lower value than the value of the control group ⑼ at the same time (p < 005). 5 From the results shown in Figure 3, it can be seen that the serum triglyceride concentration of the present invention group (2) and the control group (0) showed a lower value at any time after ingestion of fatty food. Based on this, it was found that ingestion of the composition of the present invention can achieve the effect of inhibiting the absorption of dietary fat. The specific aspect of the composition for blocking dietary fat absorption of the present invention is not shown as an example. Example 1-5 Preparation of tablet form The ingredients shown in Table 6 were mixed into a predetermined ratio (%), and 500 g of the obtained mixture was formed into a bond form by a direct powder compression method to obtain about 15 750 to 25 ⑼ ( 200-650 mg per spin) of the composition of the present invention. 35 200533300 Table 5 Examples of raw materials Example Examples Example Examples 1 2 3 4 5 Chitosan 20 13 15 18 9 Guanhua Bean Gum 60 40 Pectin 46 Locust _SL Gum 54 Gum Cloud 36 Lactose 6 7 10 Microcrystalline cellulose 10 43 20 15 40 Corn starch 17 Glycerin fatty acid esters 4 4 Sucrose fatty acid esters 3 2 Vegetable fats and oils 5 Microparticles of silica 1 1 1 1 1 Total (mg) 200 300 650 250 320

Example 6 Preparation of crackers 5 Using appropriate amount of water, 4 parts of Guanhua bean gum, 1 part of chitosan for food, 70 parts of wheat flour, 5 parts of sugar, 12 parts of fat, and appropriate amount of swelling: agent, small amount Salt and spices are mixed to make a cracker material. After the material is formed into an appropriate size, it is baked in an oven at 180 ° C for about 10 minutes to obtain a composition of the present invention in the form of a wafer. 10 Example 7 Preparation of hard biscuits Using an appropriate amount of water, 3 parts of locust bean gum, 1 part of chitosan for food, 70 parts of wheat flour, 13 parts of sugar, 10 parts of fat, and appropriate amount of bulking agent and flavor are mixed. Made of hard biscuit material. After the material is formed into an appropriate size, 36 200533300 is baked in an oven at 180 ° C for about 10 minutes' to obtain a composition of the present invention in the form of a hard cookie. Example 8 Preparation of milk sugar 5 5 parts of pectin, 1 part of chitosan for food, 30 parts of sugar and 30 parts of sweetened milk were suspended in an appropriate amount of water to prepare a solution. In addition, an appropriate amount of an emulsifier was added to 5 parts of oils and fats, and the mixture was heated and melted to prepare a liquid B. Add liquid B to liquid A, homogenize and prepare an emulsion under heating, and cook until the emulsion reaches a predetermined moisture content. After cooling, 10 parts of fondant and 10 appropriate amount of spices were added, and the mixture was stirred with a kneader. The milk sugar material after mixing is formed into an appropriate size to obtain the composition of the present invention in the form of milk sugar. Example 9 Preparation of corn thick soup 1 part of Guanhua bean gum and 0.2 parts of chitosan for food were suspended in 75 parts of milk. This suspension was mixed with 20 parts of corn (boiled) and seasonings, and wet-pulverized, and then heat-sterilized to obtain a composition of the present invention in the form of a corn soup. Examples 10 and 11 Modification of the form of high-protein flowing foods Each component shown in Table 6 in a predetermined ratio (◦ / 〇) was used to prepare the composition 20 of the present invention. 37 200533300 Table 6 Example No. Example 10 Example 11 Chitosan 0.1 0.5 Guanhua Bean Gum 0.5 — Pectin — 1.5 Complex Protein 6.9 6.7 Sodium Protein — 2.2 Casein Calcium 3.3 3.3 Whole Milk Powder 3.9 — Skim Milk powder 1.5 — Gelatin — 1.4 Enzyme-degraded gelatin 2.2 — Cheese 2.0 — Refined white sugar 2.7 3.0 m oil — 5.0 NaOH 0.1 0.1 MgS〇4 0.1 0.1 CaCl2 0.1 0.1 Fat soluble vitamins moderate amount Water soluble vitamins moderate amount Flavor appropriate amount

That is, sodium caseinate, calcium caseinate, gelatin, enzyme-degraded gelatin, cheese (protein component) and sugars (refined white sugar) were put into water, stirred and dissolved for 5 times, and then chitosan and Guanhua were added to the mixture Bean gum (or pectin) and an appropriate amount of salt are stirred and dissolved to form a liquid (liquid A). In addition, casein was added to water, and NaOH was added to neutralize and dissolve. Then, whole milk powder, skimmed milk powder, MgS04, CaCl2, fat-soluble vitamins, and rice oil were added, and the mixture was stirred and dissolved to prepare a liquid (liquid B). Mix the obtained liquid A and liquid B, adjust the amount of liquid 10 after stirring, then add water-soluble vitamins and flavors to emulsify, and fill the emulsion 38 200533300 in a 8GmL capacity tubular container, and then kill the g to produce a high protein flow. The composition of the present invention in the form of food. Example 12: The shape of frozen beverages: 0_2 parts of chitosan and appropriate sugar and sour flavor (plus / jnL / combined solution), and the obtained liquid was heated and sterilized and filled into Shao pig bags. The composition of the present invention was obtained in the form of a green beverage.

Preparation of granules 10 Tongguan Huadan Gum, 75 parts of chitosan for food, add an appropriate amount of water and mix to obtain a granular material. The material is extruded and granulated, and then dried to obtain the composition of the present invention in the form of particles. Industrial Applicability The present invention provides a composition capable of achieving an excellent fat accumulation inhibiting effect 15 (fat absorption inhibiting effect). The composition is useful as a functional preparation and a food and beverage product. [Brief description of the figure] Figure 1 is a graph showing the test results of Experimental Example 3 (horizontal axis: time after withdrawal (hours), vertical axis: triglyceride concentration (mg / dL)). 20 Figure 2 is a bar graph showing the test results of Experimental Example 4 (vertical axis: liver triglyceride amount (mg / g liver). Figure 3 is a graph showing the test according to Experimental Example 5 Result of the obtained serum triglyceride concentration (horizontal axis: time (time) after ingestion of fatty food, vertical axis: serum triglyceride concentration (mg / dL)). 39 200533300 [Explanation of Symbols of Main Components] (No )

40

Claims (1)

200533300 X. Scope of patent application: 1.-a composition for hindering the absorption of dietary fat, containing: chitosan; and ⑺ water-soluble dietary fiber, which is selected from Guar G · , Pectin (-η), Tara Gum, and Locust Bean Gum 5 Gum). Less 2. For example, the scope of the application for patent ^ "Materials that inhibit the absorption of food and food", wherein the water button dietary fiber is at least one selected from the group consisting of Lihua bean gum, fruit extract, and locust bean gum. 3. For example, in the patent application, item 1 "Techniques that hinder the absorption of Teng Shi Lai into 10 products, in which the water-soluble dietary fiber is Guanhua bean gum. 4. As described in item 1 of the scope of the patent application, which is used to hinder the absorption of dietary fat, it contains ⑺ water-soluble dietary fiber in a proportion of M0 parts by weight with respect to m parts by weight of ⑴Chitin. The water-soluble dietary fiber is at least one selected from the group consisting of Guanhuali gum, pectin, ganoderma gum, and locust bean gum. 5. For example, the winding for obstructing the absorption of dietary fat in item 1 of the scope of patent application, which contains 3 to 7 parts by weight with respect to the contained (1) chitosan 丨 parts by weight (2) water-soluble Food, weaving, and the water-soluble dietary fiber is at least one member selected from the group consisting of Guanhua ugly gum, pectin, thorn cloud gum, and locust bean gum. 6. For example, the composition for blocking dietary lin absorption in item 3 of the scope of patent application contains 3 to 7 parts by weight of Guanhua Chousheng with respect to (1) parts by weight of chitosan. 7. The composition for obstructing the absorption of dietary fat according to item 1 of the scope of patent application 41 200533300 The substance 'presents a form suitable for oral administration. 8. The composition for blocking the absorption of dietary fat according to item 1 of the scope of patent application, which has a form of food and drink, and the form of the food and drink is selected from the group consisting of snacks, beverages, and supplements Group. 5 9 · A method for hindering the absorption of dietary fat in a person who needs to block the absorption of dietary fat, is to make the human body orally ingest an effective amount of the composition in the scope of patent application No. 1. 10 · —A method for excreting dietary fat in a human body that requires dietary fat to be excreted from the body, which is a composition for which the human body ingests an effective amount of a patent claim 1 item. . 11. An anti-obesity method for a human body in need of anti-obesity, which is to make the human body orally ingest an effective amount of the composition in the scope of patent application item 1. 12. Use of the composition of the scope of patent application No. 1 is for those who use the composition to hinder the absorption of dietary fat. 15 13 · A use of the composition in the scope of patent application No. 1 is for the use of the composition to excrete dietary fat from the body. 14. A use of the composition of claim 1 in the scope of patent application is to use the composition for anti-obesity. 15 · —Species (1) The use of chitosan and (magic water-soluble dietary fiber) is to use the 20 and other components to manufacture the composition of the first patent application scope, wherein the water-soluble dietary fiber is selected from At least one of the group consisting of Chinese bean gum, pectin, thorn cloud gum, and locust ugly gum. 42