TW200502248A - Process for decreasing aggregate levels of pegylated protein - Google Patents

Process for decreasing aggregate levels of pegylated protein

Info

Publication number
TW200502248A
TW200502248A TW092126051A TW92126051A TW200502248A TW 200502248 A TW200502248 A TW 200502248A TW 092126051 A TW092126051 A TW 092126051A TW 92126051 A TW92126051 A TW 92126051A TW 200502248 A TW200502248 A TW 200502248A
Authority
TW
Taiwan
Prior art keywords
pegylated protein
aggregate levels
decreasing aggregate
decreasing
desired pegylated
Prior art date
Application number
TW092126051A
Other languages
Chinese (zh)
Other versions
TWI318629B (en
Inventor
Denis M Boyle
John J Buckley
Gary V Johnson
David E Steinmeyer
Michele Toal
Serdar Aykent
Anurag S Rothore
Original Assignee
Pharmacia Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/646,798 external-priority patent/US20040048315A1/en
Application filed by Pharmacia Corp filed Critical Pharmacia Corp
Publication of TW200502248A publication Critical patent/TW200502248A/en
Application granted granted Critical
Publication of TWI318629B publication Critical patent/TWI318629B/en

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  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention is directed generally to recombinant methods for making a desired pegylated protein and pooling of same. These method(s) yield a polypeptide product containing reduced levels of aggregate thereof pooled to provide the desired pegylated isoforms thereof.
TW92126051A 2002-09-20 2003-09-22 Process for decreasing aggregate levels of pegylated protein TWI318629B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41222702P 2002-09-20 2002-09-20
US10/646,798 US20040048315A1 (en) 2002-08-28 2003-08-25 Method for the preparation of growth hormone and antagonist thereof having lower levels of isoform impurities thereof

Publications (2)

Publication Number Publication Date
TW200502248A true TW200502248A (en) 2005-01-16
TWI318629B TWI318629B (en) 2009-12-21

Family

ID=35864143

Family Applications (1)

Application Number Title Priority Date Filing Date
TW92126051A TWI318629B (en) 2002-09-20 2003-09-22 Process for decreasing aggregate levels of pegylated protein

Country Status (3)

Country Link
AR (1) AR041350A1 (en)
RU (1) RU2368619C2 (en)
TW (1) TWI318629B (en)

Also Published As

Publication number Publication date
TWI318629B (en) 2009-12-21
AR041350A1 (en) 2005-05-11
RU2005113152A (en) 2005-10-27
RU2368619C2 (en) 2009-09-27

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MM4A Annulment or lapse of patent due to non-payment of fees