WO2002081708A1 - Process for producing thiol protease d3 - Google Patents

Process for producing thiol protease d3 Download PDF

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Publication number
WO2002081708A1
WO2002081708A1 PCT/JP2002/003337 JP0203337W WO02081708A1 WO 2002081708 A1 WO2002081708 A1 WO 2002081708A1 JP 0203337 W JP0203337 W JP 0203337W WO 02081708 A1 WO02081708 A1 WO 02081708A1
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecule
thiol protease
δprod3
analog
Prior art date
Application number
PCT/JP2002/003337
Other languages
French (fr)
Japanese (ja)
Inventor
Nami Nakamura
Tomohiro Kodera
Noriki Nio
Original Assignee
Ajinomoto Co., Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co., Inc. filed Critical Ajinomoto Co., Inc.
Priority to JP2002580071A priority Critical patent/JPWO2002081708A1/en
Publication of WO2002081708A1 publication Critical patent/WO2002081708A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

It is intended to quicken autolysis without lowering the activity of thiol protease D3 to thereby shorten the activation treatment time. A process for producing matured thiol protease D3 or its analog characterized by: i) setting ΔproD3, which has a sequence derived from the N-terminal prosequence of proD3 containing the full N-terminal prosequence of thiol protease D3 by deletion of a part thereof, or its analog under acidic conditions; and ii) recovering a protein having a protease activity which is the product obtained in the step i). Moreover, ΔproD3 having a sequence derived from the prosequence of D3 by deletion of a part thereof and its analog which are suitable for the above process, a nucleic acid molecule encoding the same, and a recombinant nucleic acid molecule containing the above nucleic acid molecule, in particular a recombinant nucleic acid molecule capable of expressing ΔproD3 in an appropriate host are provided.
PCT/JP2002/003337 2001-04-05 2002-04-03 Process for producing thiol protease d3 WO2002081708A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2002580071A JPWO2002081708A1 (en) 2001-04-05 2002-04-03 Method for producing thiol protease D3

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001107426 2001-04-05
JP2001-107426 2001-04-05

Publications (1)

Publication Number Publication Date
WO2002081708A1 true WO2002081708A1 (en) 2002-10-17

Family

ID=18959752

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/003337 WO2002081708A1 (en) 2001-04-05 2002-04-03 Process for producing thiol protease d3

Country Status (2)

Country Link
JP (1) JPWO2002081708A1 (en)
WO (1) WO2002081708A1 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09121870A (en) * 1994-12-29 1997-05-13 Ajinomoto Co Inc Dna capable of coding new thiol protease and production of the same thiol protease using the dna
JPH09294583A (en) * 1996-03-08 1997-11-18 Ajinomoto Co Inc Aminopeptidase gx and hydrolysis of protein using the same
JP2000083695A (en) * 1998-09-16 2000-03-28 Ajinomoto Co Inc Production of low-bitter peptide

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09121870A (en) * 1994-12-29 1997-05-13 Ajinomoto Co Inc Dna capable of coding new thiol protease and production of the same thiol protease using the dna
JPH09294583A (en) * 1996-03-08 1997-11-18 Ajinomoto Co Inc Aminopeptidase gx and hydrolysis of protein using the same
JP2000083695A (en) * 1998-09-16 2000-03-28 Ajinomoto Co Inc Production of low-bitter peptide

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE MEDLINE [online] NATIONAL LIBRARY OF MEDICINE; XP002952688, Database accession no. 10536045 *
SUSUMU Y. TAKAHASHI ET AL.: "Autolytic activation mechanism of bombyx acid cysteine protease (BCP)", BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, vol. 42, no. 3, 1997, pages 591 - 600, XP002952687 *
YAMAMOTO, Y. ET AL.: "Proregion of bombyx mori cysteine proteinase functions as an intramolecular chaperone to promote proper folding of the mature enzyme, abstract", ARCH. INSECT. BIOCHEM. PHYSIOL., vol. 42, no. 3, 1999, pages 167 - 178 *

Also Published As

Publication number Publication date
JPWO2002081708A1 (en) 2004-07-29

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