TR201913977A1 - Stromal vasküler fraksi̇yon elde etmek üzere bi̇r yöntem - Google Patents

Stromal vasküler fraksi̇yon elde etmek üzere bi̇r yöntem Download PDF

Info

Publication number
TR201913977A1
TR201913977A1 TR2019/13977A TR201913977A TR201913977A1 TR 201913977 A1 TR201913977 A1 TR 201913977A1 TR 2019/13977 A TR2019/13977 A TR 2019/13977A TR 201913977 A TR201913977 A TR 201913977A TR 201913977 A1 TR201913977 A1 TR 201913977A1
Authority
TR
Turkey
Prior art keywords
syringe
oil
fraction
stromal vascular
gaps
Prior art date
Application number
TR2019/13977A
Other languages
English (en)
Inventor
Veysel Doğruok Ti̇mur
Özovali Sali̇h
Original Assignee
T Biyoteknoloji Laboratuvar Estetik Medikal Kozmetik San Tic Ltd Sti
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by T Biyoteknoloji Laboratuvar Estetik Medikal Kozmetik San Tic Ltd Sti filed Critical T Biyoteknoloji Laboratuvar Estetik Medikal Kozmetik San Tic Ltd Sti
Priority to TR2019/13977A priority Critical patent/TR201913977A1/tr
Priority to PCT/TR2020/050306 priority patent/WO2021054909A1/en
Priority to EP20864767.7A priority patent/EP3876954A4/en
Publication of TR201913977A1 publication Critical patent/TR201913977A1/tr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/56—Labware specially adapted for transferring fluids
    • B01L3/563—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
    • B01L3/5635—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors connecting two containers face to face, e.g. comprising a filter
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration

Abstract

Buluş, donörden yağ dokusu içeren numunenin alınması; numenin, kan, yağ ve trigliserit olarak ayrışana kadar santrifüje edilmesi; yağ kısmının bir şırıngaya aktarılması; bahsedilen şırınganın bir yağ parçalama kitiyle bir başka şırıngaya birbirlerine uç kısımdan, yağın bir şırıngadan diğer şırıngaya yağ parçalama kitindeki bıçakların arasından aktarılmasına izin verecek şekilde irtibatlanması ve burada bahsedilen bıçağın boşluklarının 50-2400 mikron arasında olması; en azından 5 kere bir şırıngadan bir diğerine bıçak boşluklarından geçmek suretiyle yağın aktarılması ve bu işlemin en az bir kere daha tekrarlanması ve burada her bir işlemde kullanılan yağ parçalama kitlerinin bıçak boşluklarının bir öncekinden daha küçük olması; elde edilen parçalanmış yağ kısmının sırasıyla çalkalanması ve santrifüj edilmesi; santrifüj sonucu bulunduğu muhafazanın dibine biriken stromal vasküler fraksiyonun toplanması adımlarını içeren yağ dokusundan stromal vasküler fraksiyon ayrıştırmaya yönelik bir yöntem ile ilgilidir.
TR2019/13977A 2019-09-16 2019-09-16 Stromal vasküler fraksi̇yon elde etmek üzere bi̇r yöntem TR201913977A1 (tr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
TR2019/13977A TR201913977A1 (tr) 2019-09-16 2019-09-16 Stromal vasküler fraksi̇yon elde etmek üzere bi̇r yöntem
PCT/TR2020/050306 WO2021054909A1 (en) 2019-09-16 2020-04-10 A method of obtaining stromal vascular fraction
EP20864767.7A EP3876954A4 (en) 2019-09-16 2020-04-10 PROCEDURE FOR OBTAINING STROMAL VASCULAR FRACTION

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
TR2019/13977A TR201913977A1 (tr) 2019-09-16 2019-09-16 Stromal vasküler fraksi̇yon elde etmek üzere bi̇r yöntem

Publications (1)

Publication Number Publication Date
TR201913977A1 true TR201913977A1 (tr) 2021-04-21

Family

ID=74883224

Family Applications (1)

Application Number Title Priority Date Filing Date
TR2019/13977A TR201913977A1 (tr) 2019-09-16 2019-09-16 Stromal vasküler fraksi̇yon elde etmek üzere bi̇r yöntem

Country Status (3)

Country Link
EP (1) EP3876954A4 (tr)
TR (1) TR201913977A1 (tr)
WO (1) WO2021054909A1 (tr)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6020196A (en) * 1996-05-09 2000-02-01 Baxter International Inc. Devices for harvesting and homogenizing adipose tissue containing autologous endothelial cells
IT1400069B1 (it) * 2010-05-20 2013-05-17 Tremolada Dispositivo e metodo per la preparazione di tessuto, in particolare tessuto adiposo per trapianto ottenuto da materiale adiposo lobulare estratto tramite liposuzione
WO2015005871A1 (en) * 2013-07-10 2015-01-15 Agency For Science, Technology And Research Method for isolating stromal vascular fraction
CN109355251A (zh) * 2018-09-20 2019-02-19 瑞梦德医药科技(北京)有限公司 脂肪组织来源复合细胞群及其制备方法与应用
WO2020101622A1 (en) * 2018-11-12 2020-05-22 T-Bi̇yoteknoloji̇ Laboratuvar Esteti̇k Medi̇kal Kozmeti̇k San Ti̇c Ltd Şti̇ Blade - filter kit developed to be used for fat transfer

Also Published As

Publication number Publication date
WO2021054909A1 (en) 2021-03-25
EP3876954A1 (en) 2021-09-15
EP3876954A4 (en) 2022-01-26

Similar Documents

Publication Publication Date Title
van Dongen et al. Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review
US20220184135A1 (en) Bone Marrow Adipose Portion Isolation Device and Methods
SundarRaj et al. Development of a system and method for automated isolation of stromal vascular fraction from adipose tissue lipoaspirate
US20200061118A1 (en) Isolation and use of human lymphoid organ-derived suppressive stromal cells
KR20160131112A (ko) 간엽줄기세포 활성화제, 활성화된 간엽줄기세포 및 그 제조 방법
TR201913977A1 (tr) Stromal vasküler fraksi̇yon elde etmek üzere bi̇r yöntem
Zanella et al. Modeling heart disease in a dish: from somatic cells to disease-relevant cardiomyocytes
Clause et al. Developing cardiac and skeletal muscle share fast-skeletal myosin heavy chain and cardiac troponin-I expression
Zhao et al. The role of slingshot-1L (SSH1L) in the differentiation of human bone marrow mesenchymal stem cells into cardiomyocyte-like cells
Nemeth et al. A practical guide to culturing mouse and human bone marrow stromal cells
Dohmen et al. The structure of a morphogenetic cytoplasm, present in the polar lobe of Bithynia tentaculata (Gastropoda, Prosobranchia)
Branco et al. From human pluripotent stem cells to 3D Cardiac microtissues: progress, applications and challenges
Li et al. CCR7 expressing mesenchymal stem cells potently inhibit graft-versus-host disease by spoiling the fourth supplemental Billingham’s tenet
Muhammad et al. Effect of dried lake salt (Kanwa) on lipid profile and heart histology of female albino rats
Rothenberg Lineage determination in the immune system
Esfahani et al. An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level
Kievits et al. A simple indirect LE-cell test with increased sensitivity
CN105002159A (zh) 提取人体/动物血液和组织dna的dna提取试剂盒
Staudenmaier et al. Optimization of Platelet Isolation and Extraction of Autogenous TGF-ß in Cartilage Tissue Engineering
WO2018160096A1 (ru) Устройство для фракционирования жировой ткани и выделения из нее стромально-васкулярной фракции для применения в регенеративной медицине
CN100999721B (zh) 从间叶干细胞产生心脏细胞的方法
Vizziello et al. A new simple and quick method for sperm preparation and selection prior to ICSI procedure
Hagberg et al. PS1: 16 The presence of autoantibodies to multiple killer cell immunoglobulin-like receptors is associated with nephritis in sle patients
CN110361509B (zh) 获取牡丹种子的油用品质评价模型的方法
Pedersen et al. PS1: 17 Lupus nephritis: severely reduced urinary dnase i levels reflect loss of renal dnase i, disease progression and may reduce the need for renal biopsies