TR201806990A2 - Bi̇yoloji̇k sivilarda yağ asi̇dleri̇ni̇ ayirma ve saptama i̇çi̇n metod - Google Patents
Bi̇yoloji̇k sivilarda yağ asi̇dleri̇ni̇ ayirma ve saptama i̇çi̇n metod Download PDFInfo
- Publication number
- TR201806990A2 TR201806990A2 TR2018/06990A TR201806990A TR201806990A2 TR 201806990 A2 TR201806990 A2 TR 201806990A2 TR 2018/06990 A TR2018/06990 A TR 2018/06990A TR 201806990 A TR201806990 A TR 201806990A TR 201806990 A2 TR201806990 A2 TR 201806990A2
- Authority
- TR
- Turkey
- Prior art keywords
- carbon
- fatty acid
- fatty acids
- albumin
- electrode
- Prior art date
Links
- 235000014113 dietary fatty acids Nutrition 0.000 title claims abstract 11
- 229930195729 fatty acid Natural products 0.000 title claims abstract 11
- 239000000194 fatty acid Substances 0.000 title claims abstract 11
- 150000004665 fatty acids Chemical class 0.000 title claims abstract 11
- 238000000034 method Methods 0.000 title claims abstract 5
- 239000013060 biological fluid Substances 0.000 title 1
- 238000001514 detection method Methods 0.000 title 1
- 238000000926 separation method Methods 0.000 title 1
- 102000009027 Albumins Human genes 0.000 claims abstract 5
- 108010088751 Albumins Proteins 0.000 claims abstract 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract 5
- 229910052799 carbon Inorganic materials 0.000 claims abstract 5
- 239000003792 electrolyte Substances 0.000 claims abstract 2
- 239000013610 patient sample Substances 0.000 claims abstract 2
- 238000005259 measurement Methods 0.000 claims 2
- 238000009825 accumulation Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 230000001154 acute effect Effects 0.000 abstract 2
- 230000001684 chronic effect Effects 0.000 abstract 2
- 239000012472 biological sample Substances 0.000 abstract 1
- 239000003575 carbonaceous material Substances 0.000 abstract 1
- 238000002848 electrochemical method Methods 0.000 abstract 1
- 230000002209 hydrophobic effect Effects 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Endocrinology (AREA)
- Food Science & Technology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Buluş; akut ya da kronik tıbbi durumlarda yağ asidi seviyesini belirleyecek ve albümin içeren biyolojik örneklerde artmış yağ asidi seviyesiyle birlikte olan tıbbi durumların tanısını koyacak metotla ilgilidir. Metod hasta örneği ile geniş yüzey alanına sahip karbon bazlı elektrodun tutucu (holder) içinde bir süre bekletilmesi (inkübasyonu) ile başlar. Albumin üzerindeki yağ asitleri ve karbon bazlı materyal arasındaki hidrofobik çekim ilişkisi nedeniyle, karbon bazlı elektrod yağ asitlerini albuminden ayırır. Sonunda ayrılan yağ asitleri karbon bazlı elektrod üzerinde tutunup/birikip bir tabaka oluşturur. Biriken yağ asidi tabakası var olan elektrod-elektrolit (örnek) ara yüz yapısını değiştirir; bu da ara yüzün elektrokimyasal yapısını (elektriksel çift tabaka kapasitansı gibi) değiştirir. Uygun elektrokimyasal ölçüm albüminden ayrılmış yağ asidi miktarını gösterir ki, bu miktar akut ya da kronik tıbbi durumların göstergesidir.
Claims (1)
- ISTEMLER Albumin içeren biyolojik sivi örneklerde yag asitlerini ayirma ve belirleme metodu olup, özelligi; - Hasta örneginin, biri genis yüzeyli karbon bazli elektrod olmak üzere en az iki elektrod içeren tutucu (holder) içine eklenmesi, - Hasta örnegindeki albüminler ile karbon bazli elektrod arasindaki temas ihtimalini arttirmak üzere örnegin belirli bir süre bekletilmesi, hidrofobik etkilesim nedeniyle, yag asitlerinin albuminden “ayrilirken” elektrod yüzeyine “tutunmaya” baslamasi. -Süregelen “ayrilma-tutunma” asamalari karbon bazli elektrod üzerinde yag asidi birikimi olusturarak, elektrod-elektrolit ara yüz yapisinin (örnegin, elektriksel çift tabaka) degisime ugramasi, elektrokimyasal teknikte ölçüm yapilmasi, ki bu ölçüm albüminden ayrilan yag asidi miktarini yansitacaktir, basindaki ve sonundaki sonuçlarinin birbiriyle karsilastirilmasiyla yorumlanmasi, Asamalarindan olusmasiyla karakterizedir.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TR2018/06990A TR201806990A2 (tr) | 2018-05-17 | 2018-05-17 | Bi̇yoloji̇k sivilarda yağ asi̇dleri̇ni̇ ayirma ve saptama i̇çi̇n metod |
| PCT/TR2019/050301 WO2019245507A2 (en) | 2018-05-17 | 2019-05-07 | Method for extraction and determination of fatty acid in a biological fluid |
| US17/056,009 US11892460B2 (en) | 2018-05-17 | 2019-05-07 | Method for extraction and determination of fatty acid in biological fluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TR2018/06990A TR201806990A2 (tr) | 2018-05-17 | 2018-05-17 | Bi̇yoloji̇k sivilarda yağ asi̇dleri̇ni̇ ayirma ve saptama i̇çi̇n metod |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| TR201806990A2 true TR201806990A2 (tr) | 2018-06-21 |
Family
ID=66999437
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TR2018/06990A TR201806990A2 (tr) | 2018-05-17 | 2018-05-17 | Bi̇yoloji̇k sivilarda yağ asi̇dleri̇ni̇ ayirma ve saptama i̇çi̇n metod |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US11892460B2 (tr) |
| TR (1) | TR201806990A2 (tr) |
| WO (1) | WO2019245507A2 (tr) |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4369250A (en) | 1981-07-31 | 1983-01-18 | Sherwood Medical Industries Inc. | Fatty acid determination |
| US6444432B1 (en) | 1997-06-13 | 2002-09-03 | Alan M. Kleinfeld | Method of detection of cardiac ischemia using fatty acid binding protein |
| US20080305550A1 (en) | 2005-01-21 | 2008-12-11 | Hollie Huff | Targets for Detection of Ischemia |
| GB2464222B (en) | 2006-02-15 | 2010-07-07 | Inverness Medical Switzerland | Analysis method |
| WO2009036376A1 (en) | 2007-09-13 | 2009-03-19 | University Of Hawaii | Fatty acid markers for the diagnosis, prognosis and management of cardiovascular disease |
| KR101660328B1 (ko) | 2014-01-14 | 2016-09-27 | 한국과학기술연구원 | 심혈관 질환 진단용 바이오 마커 |
| WO2017116358A1 (en) | 2015-12-28 | 2017-07-06 | Oran Ismail | Method and apparatus for determination of fatty acid markers using electrical impedance measurement |
-
2018
- 2018-05-17 TR TR2018/06990A patent/TR201806990A2/tr unknown
-
2019
- 2019-05-07 WO PCT/TR2019/050301 patent/WO2019245507A2/en active Application Filing
- 2019-05-07 US US17/056,009 patent/US11892460B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2019245507A2 (en) | 2019-12-26 |
| US11892460B2 (en) | 2024-02-06 |
| US20210255208A1 (en) | 2021-08-19 |
| WO2019245507A3 (en) | 2020-03-19 |
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