TR200705989T2 - Bitki rejenerasyonu, böceğe dirençli transgenik bamyanın transformasyonu ve üretimi yöntemleri. - Google Patents

Bitki rejenerasyonu, böceğe dirençli transgenik bamyanın transformasyonu ve üretimi yöntemleri.

Info

Publication number
TR200705989T2
TR200705989T2 TR2007/05989T TR200705989T TR200705989T2 TR 200705989 T2 TR200705989 T2 TR 200705989T2 TR 2007/05989 T TR2007/05989 T TR 2007/05989T TR 200705989 T TR200705989 T TR 200705989T TR 200705989 T2 TR200705989 T2 TR 200705989T2
Authority
TR
Turkey
Prior art keywords
okra
transformation
plants
production methods
regeneration
Prior art date
Application number
TR2007/05989T
Other languages
English (en)
Inventor
Barwale Zehr Usha
Govindrao Deole Satish
Narendran Nair Madhavan
Original Assignee
Maharashtra Hybrid Seeds Company Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Maharashtra Hybrid Seeds Company Ltd. filed Critical Maharashtra Hybrid Seeds Company Ltd.
Publication of TR200705989T2 publication Critical patent/TR200705989T2/tr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Mevcut buluş, Abelmoschus türlerinden, tercihen A. esculenrus'tan elde edilen eksplantlardan bütün bitkinin rejenerasyonuna ilişkin yöntemler sunar. Ek olarak mevcut buluş ayrıca rekombinant Agrobakteri susu kullanılarak veya eksplantlarm, ilgili DNA sekansı ile kaplanmış tungsten veya altın partikülleri bombardımanına tutulması yoluyla bamya bitkisinin, bitki hücrelerinin ve dokularının transformasyonu için yöntemler sunar. Embriyoları, emdirilmiş bamya tohumlarından izole etmenin verimli bir yöntemi de tarif edilmiş olup, bamya bitkilerinin verimli şekilde rejenerasy onu ve transformasyonu için plumula ucunun genç bölünür hücrelerinin kullanılmasını sağlar.Ayrıca transforme edilmiş bamya bitkileri, bitki hücreleri ve dokuları, geliştirilmiş agronomik/agronomik olmayan özellikler ve böcek direnci için marker bazlı veya marker içermeyen sistemler kullanılarak üretilir.
TR2007/05989T 2004-08-20 2005-08-16 Bitki rejenerasyonu, böceğe dirençli transgenik bamyanın transformasyonu ve üretimi yöntemleri. TR200705989T2 (tr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IN1522DE2004 2004-08-20

Publications (1)

Publication Number Publication Date
TR200705989T2 true TR200705989T2 (tr) 2008-01-21

Family

ID=35614643

Family Applications (2)

Application Number Title Priority Date Filing Date
TR2007/05989T TR200705989T2 (tr) 2004-08-20 2005-08-16 Bitki rejenerasyonu, böceğe dirençli transgenik bamyanın transformasyonu ve üretimi yöntemleri.
TR2007/01282T TR200701282T1 (tr) 2004-08-20 2005-08-16 Bitki rejenerasyonu, böceğe dirençli transgenik bamyanın transformasyonu ve üretimi yöntemleri.

Family Applications After (1)

Application Number Title Priority Date Filing Date
TR2007/01282T TR200701282T1 (tr) 2004-08-20 2005-08-16 Bitki rejenerasyonu, böceğe dirençli transgenik bamyanın transformasyonu ve üretimi yöntemleri.

Country Status (6)

Country Link
US (2) US8067673B2 (tr)
EG (1) EG26694A (tr)
MX (1) MX2007002083A (tr)
MY (1) MY176879A (tr)
TR (2) TR200705989T2 (tr)
WO (1) WO2006018857A2 (tr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120102831A1 (en) * 2010-11-03 2012-05-03 King Abdul Aziz City For Science And Technology A method for germination of haloxylon persicum
CN104082005B (zh) * 2014-07-11 2016-01-06 四川农业大学 魔芋和黄葵的间作栽培方法
WO2016189543A1 (en) 2015-05-25 2016-12-01 Rasi Seeds (P) Ltd. Regeneration and genetic transformation of okra through somatic embryogenesis
CN106818114A (zh) * 2016-12-20 2017-06-13 蚌埠市渔歌农业科技有限公司 一种提高黄秋葵营养的种植方法
CN107969332B (zh) * 2017-11-27 2020-11-03 长春大学 天鹅绒竹芋根茎不定芽快速诱导法
CN109652360B (zh) * 2019-01-11 2022-04-15 成都大学 一种获得高生物产量的羌活细胞培养方法
CN111919753B (zh) * 2020-09-08 2022-05-06 安徽农业大学 一种番茄组培用培养基及其应用
CN113234754B (zh) * 2021-04-26 2023-03-07 北京林业大学 一种以金花葵种胚为外植体的高效遗传转化方法
CN115885845B (zh) * 2022-03-21 2023-06-20 龙岩市农业科学研究所 一种快速诱导黄秋葵不定芽扩繁的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5739409A (en) * 1987-06-19 1998-04-14 The Regents Of The University Of California Endogenously sweetened transgenic plant products
US6753463B1 (en) * 1987-11-18 2004-06-22 Mycogen Corporation Transformed cotton plants
US6762347B1 (en) * 1999-07-12 2004-07-13 James Giovannoni NOR gene compositions and methods for use thereof
GB0100105D0 (en) 2001-01-04 2001-02-14 Leuven K U Res & Dev The use of plant TPS (Trehalose-6-phosphate syntase) as a selectable marker for plant transformation

Also Published As

Publication number Publication date
US20120079624A1 (en) 2012-03-29
TR200701282T1 (tr) 2007-06-21
US8697445B2 (en) 2014-04-15
US8067673B2 (en) 2011-11-29
WO2006018857A2 (en) 2006-02-23
EG26694A (en) 2014-06-11
MY176879A (en) 2020-08-25
WO2006018857A3 (en) 2006-10-05
MX2007002083A (es) 2007-10-10
US20090025099A1 (en) 2009-01-22

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