TR200103580T2 - Agrobacterium-vasıtasıyla yaprak sapı eksplantları kullanılarak pamuğun yüksek etkinlikle dönüştürülmesi - Google Patents

Agrobacterium-vasıtasıyla yaprak sapı eksplantları kullanılarak pamuğun yüksek etkinlikle dönüştürülmesi

Info

Publication number
TR200103580T2
TR200103580T2 TR2001/03580T TR200103580T TR200103580T2 TR 200103580 T2 TR200103580 T2 TR 200103580T2 TR 2001/03580 T TR2001/03580 T TR 2001/03580T TR 200103580 T TR200103580 T TR 200103580T TR 200103580 T2 TR200103580 T2 TR 200103580T2
Authority
TR
Turkey
Prior art keywords
agrobacterium
petiole
cotton
exogenous gene
explants
Prior art date
Application number
TR2001/03580T
Other languages
English (en)
Inventor
Xian Chen Zhi
Zhang Lianhui
Original Assignee
Institute Of Molecular Agrobiology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute Of Molecular Agrobiology filed Critical Institute Of Molecular Agrobiology
Publication of TR200103580T2 publication Critical patent/TR200103580T2/tr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Yaprak sapi dokusunun Agrobacterium vasitali dönüsümü yoluyla transgenik pamuk bitkisi üretmek için bir yöntem ifsa edilmistir. Yöntem asagidaki adimlari ihtiva eder: (a) pamuk yaprak sapi eksplantlari elde etmek: (b) yaprak sapi eksplantlarini bir dissal gen ve seçilebilir bir isaret ihtiva eden bir vektör bulunduran Agrobacterium tumefaciens kültürüne tabi tutmak; Agrobacterium dissal genin ve ayiklama ajani direnç geninin yaprak sapi eksplanti hücrelerinin genomuna karali transferini gerçeklestirebilir; (c) yaprak sapi eksplantlarini kalus olusumu dogurtmak üzere yetistirmek; (d) dissal geni sentezleyen kalusu ayiklamak; (e) embriyoit olusumu dogurtmak üzere kalusu süspansiyon kültürü içinde yetistirmek; (f) embriyoitleri tam transgenik pamuk bitkileri olarak islah etmek.
TR2001/03580T 1999-06-11 1999-06-11 Agrobacterium-vasıtasıyla yaprak sapı eksplantları kullanılarak pamuğun yüksek etkinlikle dönüştürülmesi TR200103580T2 (tr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/SG1999/000058 WO2000077230A1 (en) 1999-06-11 1999-06-11 High-efficiency agrobacterium-mediated transformation of cotton using petiole explants

Publications (1)

Publication Number Publication Date
TR200103580T2 true TR200103580T2 (tr) 2002-04-22

Family

ID=20430220

Family Applications (1)

Application Number Title Priority Date Filing Date
TR2001/03580T TR200103580T2 (tr) 1999-06-11 1999-06-11 Agrobacterium-vasıtasıyla yaprak sapı eksplantları kullanılarak pamuğun yüksek etkinlikle dönüştürülmesi

Country Status (12)

Country Link
US (1) US7592508B1 (tr)
EP (1) EP1194579B1 (tr)
JP (1) JP4463456B2 (tr)
CN (1) CN100370031C (tr)
AT (1) ATE449854T1 (tr)
AU (1) AU782198C (tr)
BR (1) BR9917361B1 (tr)
DE (1) DE69941732D1 (tr)
ES (1) ES2335947T3 (tr)
MX (1) MXPA01012750A (tr)
TR (1) TR200103580T2 (tr)
WO (1) WO2000077230A1 (tr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7560611B2 (en) 2003-08-05 2009-07-14 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues
US8030544B2 (en) 2007-03-09 2011-10-04 Monsanto Technology Llc Methods for plant transformation using spectinomycin selection
CN104703998B (zh) 2012-03-13 2020-08-21 先锋国际良种公司 植物中雄性育性的遗传减少
AU2013232334A1 (en) 2012-03-13 2014-10-02 E. I. Dupont De Nemours & Company Genetic reduction of male fertility in plants
WO2013138354A1 (en) 2012-03-13 2013-09-19 Pioneer Hi-Bred International, Inc. Genetic reduction of male fertility in plants
CN102648697A (zh) * 2012-04-12 2012-08-29 山西省农业科学院棉花研究所 一种转基因棉花胚性愈伤分化培养基
KR102105166B1 (ko) * 2018-06-20 2020-04-28 두리화장품 주식회사 솜다리의 기내배양을 이용한 대량 생산방법 및 솜다리 추출물을 유효성분으로 포함하는 항산화, 항염증 및 항주름용 조성물
CN111321164A (zh) * 2020-03-12 2020-06-23 山西省农业科学院棉花研究所 一种降低棉花转基因材料泛菌的方法
CN112852863B (zh) * 2021-02-03 2022-09-30 中国林业科学研究院林业研究所 一种文冠果花瓣瞬时转化方法及其应用
CN113462719A (zh) * 2021-07-20 2021-10-01 中国农业科学院棉花研究所 一种农杆菌介导的棉花遗传转化方法
CN114574422B (zh) * 2022-03-31 2023-12-12 河北省农林科学院生物技术与食品科学研究所 一种诱导小麦花药出愈的培养基

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5004863B2 (en) * 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
IL88266A (en) * 1987-11-18 1998-03-10 Phytogen A method for regenerating a cotton plant from somatic cotton cells
EP0317511A3 (de) 1987-11-18 1991-10-16 Ciba-Geigy Ag Insektizide Baumwollpflanzenzellen
US5846797A (en) * 1995-10-04 1998-12-08 Calgene, Inc. Cotton transformation
ZA974176B (en) * 1996-05-16 1998-09-01 Univ North Texas A rapid in-vitro regeneration scheme of cotton (gossypium hirsutum l.) plants compatible with agrobacterium-mediated transformation
WO2000036911A2 (en) * 1998-12-18 2000-06-29 Monsanto Technology Llc Method for the regeneration of cotton

Also Published As

Publication number Publication date
CN1352692A (zh) 2002-06-05
EP1194579A1 (en) 2002-04-10
ATE449854T1 (de) 2009-12-15
CN100370031C (zh) 2008-02-20
AU4817099A (en) 2001-01-02
MXPA01012750A (es) 2002-07-02
EP1194579B1 (en) 2009-11-25
ES2335947T3 (es) 2010-04-06
BR9917361B1 (pt) 2011-05-31
AU782198B2 (en) 2005-07-07
JP2003502050A (ja) 2003-01-21
WO2000077230A1 (en) 2000-12-21
AU782198C (en) 2006-05-11
JP4463456B2 (ja) 2010-05-19
BR9917361A (pt) 2002-03-05
US7592508B1 (en) 2009-09-22
DE69941732D1 (de) 2010-01-07

Similar Documents

Publication Publication Date Title
RU2389797C2 (ru) Улучшенная трансформация сои
Chaudhury et al. Agrobacterium tumefaciens-mediated high frequency genetic transformation of an Indian cowpea (Vigna unguiculata L. Walp.) cultivar and transmission of transgenes into progeny
Ganapathi et al. Agrobacterium-mediated transformation of embryogenic cell suspensions of the banana cultivar Rasthali (AAB)
Gould et al. Adaptation of cotton shoot apex culture to Agrobacterium-mediated transformation
Zambryski et al. The use of the Ti plasmid of Agrobacterium to study the transfer and expression of foreign DNA in plant cells: new vectors and methods
WO1998017813A1 (fr) Procede de transformation de riz de type indica
Li et al. An improved protocol for Agrobacterium-mediated transformation of grapevine (Vitis vinifera L.)
Meilan et al. Poplar (Populus spp.)
TR200103580T2 (tr) Agrobacterium-vasıtasıyla yaprak sapı eksplantları kullanılarak pamuğun yüksek etkinlikle dönüştürülmesi
US5567599A (en) Method for producing transformed chrysanthemum plants
Mekala et al. Optimization of Agrobacterium-mediated genetic transformation of shoot tip explants of green gram (Vigna radiata (L.) Wilczek)
Zhou et al. High rooting frequency and functional analysis of GUS and GFP expression in transgenic Medicago truncatula A17
Chen et al. Agrobacterium-mediated genetic transformation of peanut and the efficient recovery of transgenic plants
Muruganantham et al. Efficient Agrobacterium-mediated transformation of Vigna mungo using immature cotyledonary-node explants and phosphinothricin as the selection agent
AU706650B2 (en) Genetic modification of plants
ZA200101818B (en) Methods for producing genetically modified plants, plant materials and plant products produced thereby.
Makhzoum et al. Novel plant regeneration and transient gene expression in Catharanthus roseus
Lewi et al. Sunflower (Helianthus annuus L.)
CN110305894B (zh) 一种快速高效的楸遗传转化方法
CA2313310A1 (en) Iris transformation method
CA2341781A1 (en) Methods for producing genetically modified plants, plant materials and plant products produced thereby
Ignacimuthu Agrobacterium mediated transformation of Vigna sesquipedalis Koern (asparagus bean)
Dogan et al. Agrobacterium mediated tumor and hairy root formation from different explants of lentils derived from young seedlings
Jia et al. Development of two protocols for Agrobacterium-mediated transformation of white clover (Trifolium repens) via the callus system
CA2361143A1 (en) Transformation and regeneration of allium plants