BR9917361A - Transformação de algodão mediada por agrobacterium de eficiência elevada empregando-se explantes de pecìolo - Google Patents

Transformação de algodão mediada por agrobacterium de eficiência elevada empregando-se explantes de pecìolo

Info

Publication number
BR9917361A
BR9917361A BR9917361-1A BR9917361A BR9917361A BR 9917361 A BR9917361 A BR 9917361A BR 9917361 A BR9917361 A BR 9917361A BR 9917361 A BR9917361 A BR 9917361A
Authority
BR
Brazil
Prior art keywords
petiole
agrobacterium
petiole explants
exogenous gene
callus
Prior art date
Application number
BR9917361-1A
Other languages
English (en)
Other versions
BR9917361B1 (pt
Inventor
Zhi Xian Chen
Lianhui Zhang
Original Assignee
Inst Of Molecular Agrobiology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Of Molecular Agrobiology filed Critical Inst Of Molecular Agrobiology
Publication of BR9917361A publication Critical patent/BR9917361A/pt
Publication of BR9917361B1 publication Critical patent/BR9917361B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Compounds Of Unknown Constitution (AREA)

Abstract

Patente de Invenção: ""TRANSFORMAçãO DE ALGODãO MEDIADA POR AGROBACTERIUM DE EFICIêNCIA ELEVADA EMPREGANDO-SE EXPLANTES DE PECìOLO". Descreve-se um método para produção de uma planta de algodão transgênica por transformação de tecido de pecíolo mediada pela Agrobacterium . O método compreende as etapas de (a) obtenção de explantes de pecíolo de algodão, (b) exposição do pecíolo a uma cultura de Agrobacterium tumefaciens abriga um vetor compreendendo um gene exógeno e um marcador selecionável, a Agrobacteríum sendo capaz de efetivar a transferência estável do gene exógeno e gene de resistência do agente de seleção para o genoma das células do explante de pecíolo, (c) cultivo dos explantes de pecíolo para induzir formação de calo, (d) seleção do calo transformado que expressa o gene exógeno, (e) cultivo do calo selecionado em cultura de suspensão para induzir a formação de embrióides, (f) regeneração dos embrióides em plantas de algodão transgênicas inteiras.
BRPI9917361-1A 1999-06-11 1999-06-11 método para produção de plantas de algodão transgênicas. BR9917361B1 (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/SG1999/000058 WO2000077230A1 (en) 1999-06-11 1999-06-11 High-efficiency agrobacterium-mediated transformation of cotton using petiole explants

Publications (2)

Publication Number Publication Date
BR9917361A true BR9917361A (pt) 2002-03-05
BR9917361B1 BR9917361B1 (pt) 2011-05-31

Family

ID=20430220

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI9917361-1A BR9917361B1 (pt) 1999-06-11 1999-06-11 método para produção de plantas de algodão transgênicas.

Country Status (12)

Country Link
US (1) US7592508B1 (pt)
EP (1) EP1194579B1 (pt)
JP (1) JP4463456B2 (pt)
CN (1) CN100370031C (pt)
AT (1) ATE449854T1 (pt)
AU (1) AU782198C (pt)
BR (1) BR9917361B1 (pt)
DE (1) DE69941732D1 (pt)
ES (1) ES2335947T3 (pt)
MX (1) MXPA01012750A (pt)
TR (1) TR200103580T2 (pt)
WO (1) WO2000077230A1 (pt)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9790512B2 (en) 2007-03-09 2017-10-17 Monsanto Technology Llc Preparation and use of plant embryo explants for transformation
US10433503B2 (en) 2003-08-05 2019-10-08 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104204209A (zh) 2012-03-13 2014-12-10 先锋国际良种公司 植物中雄性育性的遗传减少
CN104703998B (zh) 2012-03-13 2020-08-21 先锋国际良种公司 植物中雄性育性的遗传减少
EA201491673A1 (ru) 2012-03-13 2015-07-30 Пайонир Хай-Бред Интернэшнл, Инк. Генетическое снижение мужской репродуктивной функции у растений
CN102648697A (zh) * 2012-04-12 2012-08-29 山西省农业科学院棉花研究所 一种转基因棉花胚性愈伤分化培养基
KR102105166B1 (ko) * 2018-06-20 2020-04-28 두리화장품 주식회사 솜다리의 기내배양을 이용한 대량 생산방법 및 솜다리 추출물을 유효성분으로 포함하는 항산화, 항염증 및 항주름용 조성물
CN111321164A (zh) * 2020-03-12 2020-06-23 山西省农业科学院棉花研究所 一种降低棉花转基因材料泛菌的方法
CN112852863B (zh) * 2021-02-03 2022-09-30 中国林业科学研究院林业研究所 一种文冠果花瓣瞬时转化方法及其应用
CN113462719A (zh) * 2021-07-20 2021-10-01 中国农业科学院棉花研究所 一种农杆菌介导的棉花遗传转化方法
CN114574422B (zh) * 2022-03-31 2023-12-12 河北省农林科学院生物技术与食品科学研究所 一种诱导小麦花药出愈的培养基

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5004863B2 (en) * 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
EP0317511A3 (de) 1987-11-18 1991-10-16 Ciba-Geigy Ag Insektizide Baumwollpflanzenzellen
IL88266A (en) * 1987-11-18 1998-03-10 Phytogen A method for regenerating a cotton plant from somatic cotton cells
US5846797A (en) * 1995-10-04 1998-12-08 Calgene, Inc. Cotton transformation
ZA974176B (en) * 1996-05-16 1998-09-01 Univ North Texas A rapid in-vitro regeneration scheme of cotton (gossypium hirsutum l.) plants compatible with agrobacterium-mediated transformation
WO2000036911A2 (en) * 1998-12-18 2000-06-29 Monsanto Technology Llc Method for the regeneration of cotton

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10433503B2 (en) 2003-08-05 2019-10-08 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues
US11212972B2 (en) 2003-08-05 2022-01-04 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues
US9790512B2 (en) 2007-03-09 2017-10-17 Monsanto Technology Llc Preparation and use of plant embryo explants for transformation
US9885053B2 (en) 2007-03-09 2018-02-06 Monsanto Technology Llc Method of meristem excision and transformation
US10584345B2 (en) 2007-03-09 2020-03-10 Monsanto Technology Llc Preparation and use of plant embryo explants for transformation
US10717983B2 (en) 2007-03-09 2020-07-21 Monsanto Technology Llc Method of meristem excision and transformation
US10920235B2 (en) 2007-03-09 2021-02-16 Monsanto Technology Llc Apparatus for the preparation and use of plant embryo explants for transformation
US11485980B2 (en) 2007-03-09 2022-11-01 Monsanto Technology, Llc Method of meristem excision and transformation
US11542514B2 (en) 2007-03-09 2023-01-03 Monsanto Technology, Llc Apparatus for the preparation and use of plant embryo explants for transformation
US11566252B2 (en) 2007-03-09 2023-01-31 Monsanto Technology, Llc Preparation and use of plant embryo explants for transformation

Also Published As

Publication number Publication date
MXPA01012750A (es) 2002-07-02
ES2335947T3 (es) 2010-04-06
ATE449854T1 (de) 2009-12-15
EP1194579B1 (en) 2009-11-25
EP1194579A1 (en) 2002-04-10
US7592508B1 (en) 2009-09-22
CN1352692A (zh) 2002-06-05
CN100370031C (zh) 2008-02-20
BR9917361B1 (pt) 2011-05-31
AU782198B2 (en) 2005-07-07
AU782198C (en) 2006-05-11
DE69941732D1 (de) 2010-01-07
AU4817099A (en) 2001-01-02
WO2000077230A1 (en) 2000-12-21
JP2003502050A (ja) 2003-01-21
TR200103580T2 (tr) 2002-04-22
JP4463456B2 (ja) 2010-05-19

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Legal Events

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B25G Requested change of headquarter approved

Owner name: INSTITUTE OF MOLECULAR AGROBIOLOGY (SG)

Free format text: SEDE ALTERADA CONFORME SOLICITADO NA PETICAO NO 020070001801/RJ DE 05/01/2007.

B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 31/05/2011, OBSERVADAS AS CONDICOES LEGAIS.

B21A Patent or certificate of addition expired [chapter 21.1 patent gazette]

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