TH9883C3 - Method of separating sweeteners from stevia leaves by adsorption techniques. - Google Patents

Abstract

DC60 (08/08/57) Process of extracting sweeteners from stevia leaves using adsorption technique. According to this invention, stevia leaves are crushed. It was then extracted with a solvent and then separated the sweetener by adsorption with resin. By soaking them in the right conditions And take the resin Spit out the sweetener By adding ethanol solution mixed with ethyl acetate solution to Before evaporation, remove the solvent. Will get sweetener from stevia leaf extract that is a clear liquid like oil It can be used as a replacement for the sweetener in the food industry. With this artificial adsorption technique, stevia leaves are ground thoroughly. It was then extracted with a solvent and then separated the sweetener by adsorption with resin. By soaking them in the right conditions And take the resin Spit out the sweetener By adding ethanol solution mixed with ethyl acetate solution before evaporation, the solvent is removed. Will get sweetener from stevia leaf extract that is a clear liquid like oil It can be used as a substitute for sweeteners in the food industry.

Claims (3)

Disclaimers (all) that will not appear on the advertisement page: 1. The method of separating sweeteners from stevia leaves by adsorption technique has the following steps: A. Adsorption of sweeteners from stevia leaf extract (Adsorption) by putting resin into In a apple shaped bottle Then add stevia extract to the volume of 50 ml. Close the bottle tightly before shaking. Using a speed of 120 rpm at room temperature for 4.5 hours and then filtered through a vacuum filter. Then rinse the resin with 150 ml of distilled water to get the resin that adsorb the sweetener. B. Desorption of the sweetener from the stevia leaf extract (Desorption) by using the resin that Through the absorption of sweeteners and put them in a apple-shaped bottle Before adding ethanol solution mixed with Ethyl acetate solution down The ratio of ethanol solution to ethyl acetate solution is 3: 1, volume 50 ml. And take it through a shaker The speed was 120 rpm at room temperature for 0.5 hours before being filtered through a vacuum filter. The resulting solution was then evaporated with a rotary evaporator at 40 degrees Celsius to obtain a sweetener from stevia leaf extract. That looks like a clear liquid like oil 2. Process of extracting sweeteners from stevia leaves by adsorption techniques according to claim 1, where the preparation of stevia leaf extract The dried stevia leaves were thoroughly ground and sifted through a 1 mm sieve. Then stevia powder was dissolved with distilled water at a ratio of 1:50 stevia powder to distilled water (weight per volume) and used for Through the shaker Using a speed of 120 rpm at a temperature of 27-32 degrees Celsius for 2 hours and then filtered through a vacuum filter. Will get stevia leaf extract 3. Process of separating sweeteners from stevia leaves by adsorption techniques according to claim 1, where resin preparation The macropore resins, which are resins with functional groups. On the dry surface, the amount of 20 grams, then add pure ethanol to flood the surface of the The resin in a sealed container is left for 24 hours, then rinsed with 200 ml of distilled water and filtered through a vacuum filter. To remove all irrelevant monomers, a resin for adsorption is obtained.