TH12734C3 - Process of extracting sweeteners from stevia leaves with chitosan adsorption. - Google Patents

Abstract

EDIT 25/10/2016 Extraction method of sweeteners from stevia leaves by extraction techniques Formation of PGS oligomers Followed by absorption techniques According to this invention, the stevia leaves are ground thoroughly. It was then extracted with a solution of 2% mass oligomers of PGS-type micelles, then precipitated and separated by sweeteners by chitosan adsorption by soaking. Left in the right state Then, chitosan adsorbed to dissolve the sweetener by adding ethanol solution. Before evaporation, the solvent is removed to obtain a sweetener from stevia leaf extract. That looks like a clear liquid like oil It has no bitter taste at all and can be used as a food sweetener. -------------------------------------------------- ------ Revise summary 25/09/2015 Extraction method of sweetener from stevia leaves by extraction technique PGS oligomers Followed by absorption techniques According to this invention Is the introduction of the grass Sweet and ground It was then extracted with a solution of 2% mass oligomers of PGS-type micelles, then precipitated and separated by sweeteners by chitosan adsorption by soaking. Left in the right state And then used chitosan to absorb sweetener By adding ethanol solution Before evaporation, remove the solvent. Will have a message Sweetened from stevia leaf extract That looks like a clear liquid like oil Which has no bitter taste and can be used as a sweetener in food -------------------------------------------------- ------------- Edit summary of invention 22/06/2016 The method of tattoo separation of sweeteners from stevia leaves by the prickness technique PGS oligomers Followed by absorption techniques According to this invention Is the introduction of the grass Sweet and ground It was then extracted with a solution of 2% mass oligomers of PGS-type micellar cell, then precipitated and separated by chitosan adsorption. In the right conditions And then used chitosan to absorb sweetener By adding ethanol solution Before evaporation, remove the solvent. Will have a message Sweetened from stevia leaf extract That looks like a clear liquid like oil Which has no bitter taste and can be used as a sweetener in food -------------------------------------------------- ---------------------------------------------- New Request Update 8 / 8/2559 Method of tattooing extract sweeteners from stevia leaves by extraction technique PGS oligomers Followed by absorption techniques According to this invention Is the introduction of the grass Sweet and ground It was then extracted with a solution of 2% mass oligomers of PGS-type micellar cell, then precipitated and separated by chitosan adsorption. In the right conditions And then used chitosan to absorb sweetener By adding ethanol solution Before evaporation, remove the solvent. Will have a message Sweetened from stevia leaf extract That looks like a clear liquid like oil Which has no bitter taste and can be used as a sweetener in food

Claims (8)

Disclaimer (all) which will not appear on the advertisement page: edit 10/25/2016 1. Process of separating sweeteners from stevia leaves by adsorption techniques. It consists of the following steps: (a) Preparation of chitosan adsorbents It was synthesized from natural chitosan to be modified among Function on dry surfaces With chemical techniques By using an acetic acid solution at a concentration of 2-4% by volume, natural chitosan was added to dissolve in an amount equal to 1: 100-120 (natural chitosan weight by volume of acetic acid solution). Stir for 16-24 hours at room temperature, then add a 70% sulphosaconic acid solution by volume equal to 100: 2.5-3.5 (by volume of natural chitosan solution per Sulfosuccinic acid solution), vigorously stirred for 18-24 hours, add crayon equal to 100: 2.5-3.5 (by weight of natural chitosan per crayon), stirring for another 18-24 hours. Then add the solution Sodium hydroxide, 1 norm, drop by drop until solution settles. The modified chitosan solution obtained was filtered and washed with distilled water at 55-65 ° C for 24-36 h. Functional modified chitosan was obtained on the dry surface (b ) To absorb the sweetener from stevia leaf extract (Adsorption) by adding chitosan adsorbent mixed with stevia leaf extract solution into the container. Close the bottle tightly. Before shaking Using a speed of 110-140 rpm at room temperature for 240-300 minutes and then filtered through a vacuum filter. Then rinse the adsorbent chitosan with distilled water to get chitosan adsorbent that absorbs the sweetener in high amounts (c) Desorption of sweetener from chitosan adsorption (Desorption) by chitosan. San absorbent that has been absorbed by the sweetener to add ethanol solution. Close the bottle tightly. And take it through a shaker Using a speed of 110-140 rpm at room temperature for 25-40 minutes before being filtered through a vacuum filter. Then remove the resulting solution to evaporate the solvent With the rotary evaporator at a temperature of 35-45 ° C, the sweetener is obtained from the substance. Extract stevia leaves Which is a clear liquid, colorless to light green, viscous, oil-like 2. The method of separating the sweetener from stevia leaves by adsorption technique according to claim 1, where the preparation of stevia leaf extract By crushing dried stevia leaves Then sifted through a 1 mm sieve, then stevia powder was extracted with a solution of PGS oligomers at a concentration of 0.5-3% by mass in grass powder ratio. Sweetened to the above solution equal to 1: 50-75 (weight by volume), heated to 65-80 ° C for 90-120 minutes, then filtered before sedimentation and filtered again. Through a vacuum filter The stevia leaf extraction solution was obtained. 3. The method of separating the sweetener from the stevia leaf by the adsorption technique according to claim 1, in which step (a) the optimal amount of cranberry was 100: 2.5 (where Chitosan Weight 4. Process of extraction of sweeteners from stevia leaves by the adsorption technique according to the 2T claim, the PGS Osigomer Prepared by ester synthesis of Palm oil or ester of rice bran oil, glycerol and succinic acid. The best ester to glycerol to succinic acid ratio was 0.01: 1: 1.5 by mole. With the adsorption technique according to claim 1, in which step (a) the concentration of acetic acid solution And weight ratio The best chitosan by volume acetic acid solution was 2% by volume and ratio 1: 100 (by weight by volume). 6. The method of separating the sweetener from stevia leaf by the adsorption technique according to the criteria. Hold the right 1 where (b) the best time for chitosan sorbent and leaf extract solution. Stevia into a shaker is 270 minutes. 7. Separation of the sweetener from stevia leaves by the adsorption technique according to claim 2, where the best concentration of the PGS-type micellar solution. Osgomer is 2 percent by mass 8. The separation method of sweeteners from stevia leaves by adsorption technique according to claim 1, where in the step (a) the optimal chitosan content For the preparation of absorbable chitosan is 20 g. ---------------------------- Revised 25/09/15 1.5-3.5 (by Weight of natural chitosan (chitosan) at a bit, stirring for another 18-24 hours, then add the solution. Sodium hydroxide, 1 norm, drop by drop until solution settles. Filtered and washed with distilled water, then treated with modified chitosan solution obtained at 55-65 ° C for 24-36 hours. Modified chitosan function group was obtained on dry surface (b ) Adsorption of sweeteners from stevia leaf extraction solution by adding chitosan adsorbent mixed with stevia leaf extract solution into the container. Close the bottle tightly before shaking it. Using a speed of 110-140 rpm at room temperature for 240-300 minutes and then filtered through a vacuum filter. Then rinse the adsorbent chitosan with distilled water to get chitosan adsorbent that absorbs the sweetener in high amounts (c) Desorption of sweetener from chitosan adsorption (Desorption) by chitosan. San Sucker The absorbent has been absorbed with a thickening agent to add ethanol solution. Close the bottle tightly and pass it through a shaker. Using a speed of 110-140 per minute at room temperature for 25-40 minutes before being filtered through a vacuum filter. Then remove the resulting solution to evaporate the solvent With the rotary evaporator at a temperature of 35-45 ° C, the sweetener is obtained from the substance. Extract stevia leaves The liquid is clear, colorless to light green, viscous, oil-like 1.-75 (weight by volume) and heated at 65-80 degrees Celsius for 90-120 minutes, then filtered. Before settling and filtering again Through vacuum Will get stevia leaf extract solution 3. The method of separating the sweetener from stevia leaf with absorbent chitosan according to claim 1, in which step (a) where the best chalk content is 100: 2.5 (by chitosan weight). Santhammachon Phong) 4. Process of extracting sweeteners from stevia leaves with chitosan adsorbent according to claim 2, where PGS oligomers Prepared by ester synthesis Of palm oil or ester of rice bran oil, glycerol and succinic acid Or its derivatives in the ratio of ester to glycerol to succinic acid, the best was 0.01: 1: 1.5 by mole. 5. The method of separating the sweetener from stevia leaves. With chitosan absorb according to claim 1, in which step (a) the concentration of acetic acid solution And chitosan weight ratio The best acetic acid solution was 2% by volume and a ratio of 1: 100 (by weight-to-volume). 6. The method of separating the sweetener from stevia leaves with chitosan adsorbed according to the claim. 1 Where (b) the best time for chitosan adsorption was mixed with leaf extract solution. Stevia into a shaker is 270 minutes 7. Process of separating the sweetener from stevia leaves with chitosan adsorbed according to claim 2, where the best concentration of the solution of the PGA-type cellular coagulant. The oligomers were 2% by mass. 8. The separation of sweetener from stevia leaves with absorbent chitosan according to claim 1, where in step (a) the chitosan content at The most suitable for the preparation of absorbable chitosan is 20 g. ------------------------------------- -------------------------------- 1.5-3.5 (by weight, Chitosan Naturally to chalk), stirring for another 18-24 hours, then add 1 norm of sodium hydroxide solution, drop by drop until the solution settles. Filtered and washed with distilled water, then treated with modified chitosan solution obtained at 55-65 ° C for 24-36 hours. Modified chitosan function group was obtained on dry surface (b ) Adsorption of sweeteners from stevia leaf extraction solution by adding chitosan adsorbent mixed with stevia leaf extract solution into the container. Close the bottle tightly before shaking it. Using a speed of 110-140 rpm at room temperature for 240-300 minutes and then filtered through a vacuum filter. Then rinse the adsorbent chitosan with distilled water to get chitosan adsorbent that absorbs the sweetener in high amounts (c) Desorption of sweetener from chitosan adsorption (Desorption) by chitosan. San Sucker The absorbent has been absorbed with a thickening agent to add ethanol solution. Close the bottle tightly and pass it through a shaker. Using a speed of 110-140 per minute at room temperature for 25-40 minutes before being filtered through a vacuum filter. Then remove the resulting solution to evaporate the solvent With the rotary evaporator at a temperature of 35-45 ° C, the sweetener is obtained from the substance. Extract stevia leaves The liquid is clear, colorless to light green, viscous, oil-like 1.-75 (weight by volume) and heated at 65-80 degrees Celsius for 90-120 minutes, then filtered. Before settling and filtering again Through vacuum Will get stevia leaf extract solution 3. The method of separating the sweetener from stevia leaf with absorbent chitosan according to claim 1, in which step (a) where the best chalk content is 100: 2.5 (by chitosan weight). Santhammachon Phong) 4. Process of extracting sweeteners from stevia leaves with chitosan adsorbent according to claim 2, where PGS oligomers Prepared by ester synthesis of Palm oil or ester of rice bran oil, glycerol and succinic acid. Or its derivatives in the ratio of ester to glycerol to succinic acid, the best was 0.01: 1: 1.5 by mole. 5. The method of separating the sweetener from stevia leaves. With chitosan absorb according to claim 1, in which step (a) the concentration of acetic acid solution And chitosan weight ratio The best acetic acid solution was 2% by volume and a ratio of 1: 100 (by weight-to-volume). 6. The method of separating the sweetener from stevia leaves with chitosan adsorbed according to the claim. 1 Where (b) the best time for chitosan adsorption was mixed with leaf extract solution. Stevia into a shaker is 270 minutes 7. Process of separating the sweetener from stevia leaves with chitosan adsorbed according to claim 2, where the best concentration of the solution of the PGA-type cellular coagulant. The oligomers were 2% by mass. 8. The separation of sweetener from stevia leaves with absorbent chitosan according to claim 1, where in step (a) the chitosan content at The most suitable for the preparation of absorbable chitosan is 20 g. ------------------------------------- --------------------------- Modified 22/06/2016 1.5-3.5 (by chitosan weight Naturally to chalk), stirring for another 18-24 hours, then add 1 norm of sodium hydroxide solution, drop by drop until the solution settles. Filtered and washed with distilled water, then treated with modified chitosan solution obtained at 55-65 ° C for 24-36 hours. Modified chitosan function group was obtained on dry surface (b ) Adsorption of sweeteners from stevia leaf extraction solution by adding chitosan adsorbent mixed with stevia leaf extract solution into the container. Close the bottle tightly before shaking it. Using a speed of 110-140 rpm at room temperature for 25-40 minutes before being filtered through a vacuum filter. Then remove the resulting solution to evaporate the solvent With the rotary evaporator at a temperature of 35-45 ° C, the sweetener is obtained from the substance. Extract stevia leaves The liquid is clear, colorless to light green, viscous, oil-like 1.-75 (weight by volume) and heated at 65-80 degrees Celsius for 90-120 minutes, then filtered. Before settling and filtering again Through vacuum Will get stevia leaf extract solution 3. The method of separating the sweetener from stevia leaf with absorbent chitosan according to claim 1, in which step (a) where the best chalk content is 100: 2.5 (by chitosan weight). Santhammachon Phong) 4. Process of extracting sweeteners from stevia leaves with chitosan adsorbent according to claim 2, where PGS oligomers Prepared by ester synthesis of Palm oil or ester of rice bran oil, glycerol and succinic acid. Or its derivatives in the ratio of ester to glycerol to succinic acid, the best was 0.01: 1: 1.5 by mole. 5. The method of separating the sweetener from stevia leaves. With chitosan absorb according to claim 1, in which step (a) the concentration of acetic acid solution And chitosan weight ratio The best acetic acid solution was 2% by volume and a ratio of 1: 100 (by weight-to-volume). 6. The method of separating the sweetener from stevia leaves with chitosan adsorbed according to the claim. 1 Where (b) the best time for chitosan adsorption was mixed with leaf extract solution. Stevia into a shaker is 270 minutes 7. Process of separating the sweetener from stevia leaves with chitosan adsorbed according to claim 2, where the best concentration of the solution of the PGA-type cellular agent The oligomers were 2% by mass. 8. The separation of sweetener from stevia leaves with absorbent chitosan according to claim 1, where in step (a) the chitosan content at The most suitable for the preparation of absorbable chitosan is 20 g. ------------------------------------- ----- New request Update 8/8/2016 1.5-3.5 (by volume of natural chitosan solution to sulfosuccinic acid solution) vigorously stirred for 18-24 hours, add crayon equal to 100 : 2.5-3.5 (by weight, Chitosan Naturally to chalk), stirring for another 18-24 hours, then add 1 norm of sodium hydroxide solution, drop by drop until the solution settles. Filtered and washed with distilled water. The resulting modified chitosan solution was baked at 55-65 ° C for 24-36 hours. Functional modified chitosan was obtained on dry surface (b ) Adsorption of sweeteners from stevia leaf extraction solution by adding chitosan adsorbent mixed with stevia leaf extract solution into the container. Close the bottle tightly before shaking it. Using a speed of 110-140 rpm at room temperature for 240-300 minutes and then filtered through a vacuum filter. Then rinse the adsorbent chitosan with distilled water to get chitosan adsorbent that absorbs the sweetener in high amounts (c) Desorption of sweetener from chitosan adsorption (Desorption) by chitosan. San Sucker Absorb the sweetener and add ethanol solution. Close the bottle tightly and pass it through a shaker. Using a speed of 110-140 rpm at room temperature for 25-40 minutes before being filtered through a vacuum filter. Then remove the resulting solution to evaporate the solvent With the rotary evaporator at a temperature of 35-45 ° C, the sweetener is obtained from the substance. Extract stevia leaves That looks like a clear, colorless liquid to a green, light, viscous oil 2. Process of extracting sweeteners from stevia leaves with absorbent titosan according to claim 1, where the preparation of stevia leaf extract By crushing the dried stevia leaves Then sifted through a 1 mm sieve. Then stevia powder was extracted with a solution of PGS oligomers, 0.5-3% concentration by mass at grass powder ratio. The sweetness of the above solution is 1: 50-75 (weight by volume), heated to 65-80 ° C for 90-120 minutes, then filtered before sedimentation and filtered again. Through a vacuum filter Will get stevia leaf extracting solution 3. The method of separating the sweetener from stevia leaf with absorbent chitosan according to claim 1, in which step (a) where the best chalk content is 100: 2.5 (by chitosan weight). Santhan to Phetchaburi) 4. Process of separating sweeteners from stevia leaves with chitosan adsorption according to claim 2, where PGS okigomer cell formation Prepared by ester synthesis of Palm oil or ester to glycerol to the best succinic acid is 0.01: 1: 1.5 by mole. 5. Process of extracting sweeteners from stevia leaves with chitosan adsorption according to claim 1, in which step (a) the concentration of acetic acid solution And chitosan weight ratio By volume, the best acetic acid solution was 2% by volume and ratio 1: 100 (weight-to-volume). 6. The method of separating sweetener from stevia leaves with chitosan adsorbent according to claim 1, where in step (b) the best time to use kotosan adsorbent mixed with leaf extract solution. Stevia into the shaker is 270 minutes. 7. The method of separating the sweetener from grass leaves with chitosan adsorbed claim 2, where the best concentration of the solution of PGS oligomers. Is 2 percent by mass 8. Process of extracting sweeteners from stevia leaves with absorbent chitosan according to claim 1, where in step (a) the optimal chitosan content for the preparation of absorbent chitosan is 20 g.