TH9468A - Modified DNA and its uses - Google Patents

Abstract

DNA Modification and Utilization: Modified DNA chromosomes containing DNA sequences responsible for operonpurine expression in Bacillus microorganisms, and DNA of the adjacent third region of those sequences, part or all of the DNA sequences responsible for operonpurine expression, which have been modified to increase operonpurine expression, have been revealed. Carriers inserted into modified DNA have also been identified. Novel Bacillus species capable of producing inosine and/or guanosine transformed by DNA or carriers, as well as improved methods for the production of inosine and/or guanosine using microorganisms, have been revealed.

Claims (12)

1. The process for producing inosine and/or guanosine involves culturing Bacillus microorganisms capable of producing inosine and/or guanosine, which are transformed by DNA chromosomes containing the DNA sequence responsible for operonpurine expression in Bacillus microorganisms and DNA from the 3rd neighboring region of that sequence. Part or all of the DNA sequence responsible for operonpurine expression is modified to increase that expression. The microorganisms are then cultured in a medium to produce and accumulate inosine and/or guanosine, and the results are harvested. 2. A procedure for the production of inosine and/or guanosine, which involves culturing Bacillus microorganisms capable of producing inosine and/or guanosine transformed by DNA according to patent 1, where the transfected DNA sequence contains regions involved in the inhibition of operonpurine expression by adenine-proximity substances, and culturing them in a culture medium to induce the production and accumulation of inosine and/or guanosine, and harvesting the resulting product. 3. A procedure for the production of inosine and/or guanosine, which involves culturing Bacillus microorganisms capable of producing inosine and/or guanosine transformed by DNA according to Patent 1, where the transfected DNA sequence contains regions involved in the inhibition of operonpurine expression by guanine-like substances, and culturing them in a culture medium to induce the production and accumulation of inosine and/or guanosine, and harvesting the results. 4. A procedure for the production of inosine and/or guanosine, which involves culturing Bacillus microorganisms capable of producing inosine and/or guanosine transformed by the DNA according to Patent 1, where the extracted DNA sequence is a promoter of operonpurine, and culturing them in a culture medium to induce the production and accumulation of inosine and/or guanosine, and harvesting the resulting product. 5. A procedure for the production of inosine and/or guanosine, consisting of culturing Bacillus microorganisms capable of producing DNA-transformed inosine and/or guanosine as per Patent 4, where the operonpurine promoter is replaced by different promoters capable of expression, culturing them in a culture medium to produce and accumulate inosine and/or guanosine, and harvesting the results. 6. A procedure for the production of inosine and/or guanosine, which involves culturing Bacillus microorganisms capable of producing inosine and/or guanosine transfected by DNA according to Patent 5, where different promoters are derived from Bacillus chromosome DNA or from Bacillus phages, culturing the microorganisms in a culture medium to produce and accumulate inosine and/or guanosine, and harvesting the resulting product. 7. A procedure for the production of inosine and/or guanosine, consisting of culturing Bacillus microorganisms capable of producing inosine and/or guanosine transformed by an DNA-embedded vector as per Patent 1, cultivating them in a culture medium to induce the production and accumulation of inosine and/or guanosine, and harvesting the resulting product. 8. A procedure for the production of inosine and/or guanosine, consisting of culturing Bacillus microorganisms capable of producing inosine and/or guanosine transformed by a DNA-embedded vector as per Patent 2, cultivating them in a culture medium to induce the production and accumulation of inosine and/or guanosine, and harvesting the resulting product. 9. A procedure for the production of inosine and/or guanosine, consisting of culturing Bacillus microorganisms capable of producing inosine and/or guanosine transformed by an DNA-embedded vector as per claim 3, cultivating them in a culture medium to induce the production and accumulation of inosine and/or guanosine, and harvesting the resulting product. 10. A procedure for the production of inosine and/or guanosine, consisting of culturing Bacillus microorganisms capable of producing inosine and/or guanosine transformed by an DNA-embedded vector as per Patent 4, cultivating them in a culture medium to induce the production and accumulation of inosine and/or guanosine, and harvesting the resulting product. 11. A procedure for the production of inosine and/or guanosine, consisting of culturing Bacillus microorganisms capable of producing inosine and/or guanosine transformed by an DNA-embedded vector as per claim 5, cultivating them in a culture medium to induce the production and accumulation of inosine and/or guanosine, and harvesting the resulting product. 12. A procedure for the production of inosine and/or guanosine, consisting of culturing Bacillus microorganisms capable of producing inosine and/or guanosine transformed by an DNA-embedded vector as per claim 6, cultivating them in a culture medium to induce the production and accumulation of inosine and/or guanosine, and harvesting the resulting product. (12 claims, 3 pages, 9 figures)