TH82884A - The genes that decipher acetate lactase - Google Patents

Abstract

DC60 (02/12/54) This invention provides a gene that encodes ALS, a protein that shows a very high degree of tolerance to the PC plant antidote. The genes of this invention encode proteins (a) or (b). ) Here (a) protein which contains the amino acid sequence of the sequence number: 1; (b) the protein which consists of the amino acid sequence which is derivable from the amino acid sequence of the sequence number: 1 by substitution. , Deletion or addition of at least one Or more than one amino acid, which is resistant to the pyrimidinylcarboxy herbicide and contains acetolactate syntase activity. The protein that encoded ALS, which exhibited a very high degree of resistance to PC anticides, this invention gene encoded the following proteins (a) or (b): (a) proteins, which consist of an amino acid sequence. Of the sequence number: 1; (b) protein, which consists of an amino acid sequence which is derivative from the amino acid sequence of the sequence number: 1 by substitution, subtraction, or addition of one or more Or more than one amino acid that is resistant to the pyrimidilcarboxy herbicide and contains acetolactate syntase activity:

Claims (7)

Disclaimers (all) that will not appear on the advertisement page: 1.Single nucleic acid containing a nucleic acid sequence encoding the protein acetolactate syntase, amino acid in the position corresponding to position 627 of acetolactate. T Strain Syntheses The primitive number 12 is isoleucine, where protein acetolactate Syntase is resistant to substances. Kills pyrimidinilcarboxy weeds and contains acetolacetate syntase activity. 2. Nucleic acids are isolated according to claim 1, while the protein is also composed of substitutions. In addition, the amino acid corresponding to position 548 of the original species of acetolactate syntase of the 12th is leucine. 3.Nucleic acids are separated according to claim 1, where the nucleic acids are coded number 12, where the amino acid at position 627 is isoleucine. 4. Nucleic acids are isolated according to claim 2, whereby the protein contains amino acid sequences. Mino's No. 1 5. A recombinant vector consisting of a nucleic acid sequence selected from a group consisting of: (a) the nucleic acid sequence that encodes the acetolactate protein. Syntase in which amino acids are positioned Corresponds to position 627 of acetolactate The original species of syntase 12 is isoleucine, where acetolactate protein The cintase is resistant to the pyrimidinylcarboxy herbicide and contains acetolacetate, syntase activity and (b) nucleic acid sequences. That encoded protein acetolactate syntase, in which the amino acids were positioned Corresponds to position 627 of acetolactate The original species of syntase 12 was isoleucine and amino acids at the position corresponding to position 548 of acetylactate. Strain Syntheses The primitive number 12 is leucine, where the protein acetolate Syntheses are resistant to Pyrimidilan Carboxy Herbicide and Acetate Syntase Activity 6.Method for cultivating plants in which isolated nucleic acids in accordance with one of the claims of Claim 1, 2, 3 or 4 and is resistant to the pyrimidinyl carboxy herbicide consisting of Plant cultivation in the presence of pyrimidilcarboxy herbicide 7.Methods for selection of transformants Cells with nucleic acid sequences selected from The group includes: (a) the nucleic acid sequence that encodes the protein acetolactate. Syntase in which amino acids are positioned Corresponds to position 627 of acetolactate The original species of syntase 12 is isoleucine, where acetolactate protein The cintase is resistant to the pyrimidinylcarboxy herbicide and contains acetolactate, syntase activity and (b) nucleic acid sequences. That encodes the protein acetolactate syntase in which the amino acid is positioned Corresponds to position 627 of acetolactate The original species of syntase 12 was isoleucine and amino acids at the position corresponding to position 548 of acetylactate. Strain Syntheses The primitive number 12 is leucine, where protein acetolactate Syntheses are resistant to Pyrimidilancarboxy herbicides and contains acetolactate syntase activity, which includes: (a) induction of nucleic acid vectors. The aforementioned ig goes into the host as himself Mark selectable and (b) host culture in the presence of pyrimidinyl carboxy herbicide.