WO1999067373A3 - Invertebrate intestinal mucins, cdnas, antibodies and their use - Google Patents

Invertebrate intestinal mucins, cdnas, antibodies and their use Download PDF

Info

Publication number
WO1999067373A3
WO1999067373A3 PCT/US1999/014220 US9914220W WO9967373A3 WO 1999067373 A3 WO1999067373 A3 WO 1999067373A3 US 9914220 W US9914220 W US 9914220W WO 9967373 A3 WO9967373 A3 WO 9967373A3
Authority
WO
WIPO (PCT)
Prior art keywords
iim
protein
dna sequence
recombinant
sequence
Prior art date
Application number
PCT/US1999/014220
Other languages
French (fr)
Other versions
WO1999067373A2 (en
Inventor
Robert R Granados
Ping Wang
Original Assignee
Thompson Boyce Plant Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/103,429 external-priority patent/US6187558B1/en
Application filed by Thompson Boyce Plant Res filed Critical Thompson Boyce Plant Res
Priority to AU47108/99A priority Critical patent/AU4710899A/en
Priority to CA002331921A priority patent/CA2331921A1/en
Publication of WO1999067373A2 publication Critical patent/WO1999067373A2/en
Publication of WO1999067373A3 publication Critical patent/WO1999067373A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/13—Immunoglobulins specific features characterized by their source of isolation or production isolated from plants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Insects & Arthropods (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Pest Control & Pesticides (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention represents the disclosure of a novel insect intestinal mucin. The IIM protein was been identified and cloned using T. ni larva. The cDNA and amino acid sequences have been determined and are disclosed. The novel protein has an approximate molecular mass of 400 kDa. These sequences are useful for the production of transgenic or recombinant vectors including viral, microorganism, cell, plant, or animals, wherein the virus, microorganism, cell, plant, or animal is the product of an insertion of a gene expression vector including a DNA that encodes an IIM protein sequence. Thereafter the engineered host of the IIM DNA sequence is capable of expressing said IIM protein in a functional form. Also useful is a purified and isolated recombinant DNA sequence comprising a DNA sequence that codes for an IIM protein. The recombinant DNA sequence used can be a cDNA sequence for either IIM14 or IIM22, SEQ. ID.'s No. 1; and 2 respectively. The current invention also provides for the use of the purified amino acid sequences of IIM14 or IIM22, SEQ. ID.'s 3 or 4 respectively. With this knowledge of the proteinaceous components of the PM, and particularly the mucin-like proteins it will be possible to enhance the effectiveness of bio-engineered pesticides, recombinant viral vectors, enhance the defenses of transgenic plants, or protect insect vectors susceptible to attack by organisms utilizing enhancin or enhancin-like enzymes.
PCT/US1999/014220 1998-06-24 1999-06-23 Invertebrate intestinal mucins, cdnas, antibodies and their use WO1999067373A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU47108/99A AU4710899A (en) 1998-06-24 1999-06-23 A novel invertebrate intestinal mucin cdna and related products and methods
CA002331921A CA2331921A1 (en) 1998-06-24 1999-06-23 A novel invertebrate intestinal mucin cdna and related products and methods

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/103,429 US6187558B1 (en) 1998-06-24 1998-06-24 Invertebrate intestinal mucin cDNA and related products and methods
US09/103,429 1998-06-24
US09/294,663 1999-04-19
US09/294,663 US6765127B1 (en) 1998-06-24 1999-04-19 Invertebrate intestinal mucin cDNA and related products and methods

Publications (2)

Publication Number Publication Date
WO1999067373A2 WO1999067373A2 (en) 1999-12-29
WO1999067373A3 true WO1999067373A3 (en) 2000-07-20

Family

ID=26800455

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/014220 WO1999067373A2 (en) 1998-06-24 1999-06-23 Invertebrate intestinal mucins, cdnas, antibodies and their use

Country Status (3)

Country Link
AU (1) AU4710899A (en)
CA (1) CA2331921A1 (en)
WO (1) WO1999067373A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL357078A1 (en) * 2000-02-04 2004-07-12 Societe Des Produits Nestle S.A. A method for maintaining or improving the synthesis of mucins
EP3415010A1 (en) 2017-06-13 2018-12-19 Agrosavfe Nv Insect-controlling polypeptides and methods

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0293249A1 (en) * 1987-05-28 1988-11-30 Amrad Corporation Limited Novel fusion protein
WO1996000783A1 (en) * 1994-06-28 1996-01-11 Ciba-Geigy Ag Antibodies which bind to insect gut proteins and their use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0293249A1 (en) * 1987-05-28 1988-11-30 Amrad Corporation Limited Novel fusion protein
WO1996000783A1 (en) * 1994-06-28 1996-01-11 Ciba-Geigy Ag Antibodies which bind to insect gut proteins and their use

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
SMITH, M.D.: "Antibody Production in Plants", BIOTECHNOLOGY ADVANCES, vol. 14, no. 3, 1996, pages 267 - 281, XP004046108 *
WANG, P. & GRANADOS, R.R.: "An intestinal mucin is the target substrate for a baculovirus enhancin", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 94, no. 13, 24 June 1997 (1997-06-24), pages 6977 - 6982, XP002125146 *
WANG, P. & GRANADOS, R.R.: "Molecular Cloning and Sequencing of a Novel Invertebrate Intestinal Mucin cDNA", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 26, 27 June 1997 (1997-06-27), pages 16663 - 16669, XP002125145 *

Also Published As

Publication number Publication date
WO1999067373A2 (en) 1999-12-29
CA2331921A1 (en) 1999-12-29
AU4710899A (en) 2000-01-10

Similar Documents

Publication Publication Date Title
Deo et al. Strategies for improving antimicrobial peptide production
US5597945A (en) Plants genetically enhanced for disease resistance
DE69433407D1 (en) ANTI-MICROBIAL PROTEINS
EP0675960A1 (en) Plants genetically enhanced for disease resistance
US7067624B2 (en) Antimicrobial proteins
EP0913469A4 (en) Floral organ-specific genes and promoter sequences thereof
AU653526B2 (en) Novel signal sequences
KR950700409A (en) Peptides with Insecticidal Effects (INSECTICIDALLY EFFECTIVE PEPTIDES)
US7276646B1 (en) Synthetic polynucleotide coding for human lactoferrin, vectors, cell and transgenic plants containing it
WO1999067373A3 (en) Invertebrate intestinal mucins, cdnas, antibodies and their use
US7081568B2 (en) Transgenic plants expressing temporin peptides
RU95106626A (en) Small rna-virus, nucleic acid, vectors, host-cell, protein or polypeptide preparation, antibodies, method of struggle against insects, transgenic plant, preparation hasv, method of hasv identification
WO2001012801A3 (en) Defense-related signaling genes and methods of use
KR100452106B1 (en) Hypersensitive response elicitor from xantomonas axonopodis and use thereof
US6534265B1 (en) Oryzacystatin-I applications and methods
AU751263B2 (en) Gene coding for androctonine, vector containing same and transformed disease-resistant plants obtained
Lee et al. Multimeric expression of the antimicrobial peptide buforin II in Escherichia coli by fusion to a cysteine-rich acidic peptide
CA2074113A1 (en) Organic compounds
AU723474B2 (en) Antimicrobial proteins
TH82884A (en) The genes that decipher acetate lactase
Destefano-Beltran et al. Use of synthetic genes to enhance nutritional quality and disease resistance in plants: application to potato
ATE226256T1 (en) ANTIFUNGAL PROTEINS, DNA CODING THEREOF, AND HOST CELLS CONTAINING THEM
Bcltrin et al. Use of Synthetic Genes to Enhance Nutritional Quality and Disease Resistance in Plants: Application to Potato
TH36371B (en) The genes that decipher acetate lactase
Kim et al. Expression of Active Antibacterial Bumblebee Abaecin in Escherichia coli Cells

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

ENP Entry into the national phase

Ref document number: 2331921

Country of ref document: CA

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase