TH81937A - Method for producing base substances - Google Patents

Abstract

DC60 (20/01/49) Method for producing base substances by cultivating microorganisms capable of producing such substances in food. Liquid inside the fermentation tank Cause to create The bases were then accumulated by fermentation in the same feed, with the total ammonia concentration adjusted within any part of the culture period. Part to be in a constant range This reduces the use of counter ions, such as sulfate ions and or chloride ions, that is, the invention is a method for producing therapeutic bases. The total ammonia content is sufficient to meet the nitrogen source requirements for the growth. Of microorganisms and product creation At the same time, it is at a level that does not cause any inhibition of growth. Or the creation of microbial products This will reduce the use of sulfate ions and chloride ions in the culture medium. A method for producing base substances by cultivating microorganisms capable of producing such substances in food. Liquid inside the fermentation tank Cause to create The bases were then accumulated by fermentation in the same feed, with the total ammonia concentration adjusted within any part of the culture period. Part to be in a constant range This reduces the consumption of counter ions, such as sulfate ions and or chloride ions, that is, the invention is a method for producing therapeutic bases. The total ammonia content is sufficient to meet the nitrogen source requirements for the growth. Of microorganisms and product creation At the same time, it is at the level that does not cause any inhibition of growth. Or the creation of microbial products This will reduce the use of sulfate ions and chloride ions in the culture medium.

Claims (1)

Disclaimers (all) that will not appear on the advertisement page: 1.Methods for producing base amino acids by fermentation that include culture. Bacteria capable of producing amino acids, bases in liquid media, which have been It is placed in a fermentation tank to produce and accumulate base amino acids in the culture medium. Where is the amount of sulfate and / or chloride Ions, which act as counters, ions of base substances are reduced By adjusting the total ammonia concentration in the culture medium during the period of being selected from Composition group: A) As the pH of the medium increases. Due to the shortage of the counter ion Derived from the accumulation of base amino acids and B) when the pH increases due to the production of cations into the medium; Where is the ammonium concentration The ions in the culture medium are valued at the sum of the equivalent Bicarbonate ions, ions and / or carbonate ions and other anions Dissolved In the culture medium was greater than the equivalent of amino acid base ions which were ionized by amino acids. No-base, which was deposited in the medium and where the total ammonium concentration As mentioned in the culture medium, the value is at a level which does not inhibit the production of base amino acids by bacteria and where such concentrations are determined first as follows. a) Bacteria are cultured in the medium at various pH values and all ammonia concentrations but within the range of 1 to 500 mmolar b) the productivity of amino acids base is measured at each value. pH and each total ammonia concentration and c) the total concentrations provided were 50% or more of the ammonium production capacity. Mino-type bases on the basis of the productivity obtained under optimal conditions were determined. For each pH value