KR102008273B1 - A composition containing deep sea water for moisturizing skin - Google Patents

Abstract

The present invention relates to a skin moisturizing composition comprising deep sea water, and more specifically, deep sea water containing manganese, calcium and potassium in a content ratio (w / v) of 2-4: 0.5-1.5: 0.5-1.5 It relates to a skin moisturizing composition comprising a.

Description

A composition containing deep sea water for moisturizing skin

The content described herein relates to the novel use of deep sea water for skin.

Deep sea water is water certified by the Ministry of Land, Transport and Maritime Affairs by the Deep Sea Water Act, which is less than 200 m from the surface of seawater where sunlight does not reach, and does not produce organic matter by photosynthesis. It is known that the deep sea water contains 60 kinds of mineral components, and researches on the manufacturing method of the food using the same are being conducted.

Deep ocean water is formed by the rapid cooling of temperatures and formation of glaciers as seawater circulates in the oceans in the colder glacial regions of the North Atlantic Greenland. Thousands of years, they exist intact with each other because of density differences with surface water.

Since the deep sea water is located 200 m or less from the surface of the sea, the concentration of organic matter is low, there is no contamination by E. coli or general bacteria, and the cleanliness is less likely to be caused by chemicals from the land or the atmosphere. In addition, its temperature is low all year round, its stability is low, and it has a low water temperature, and since it is water formed for thousands of years, its properties are stable, and it is matured due to the action of various enzymes.

Deep seawater is widely used in the food field, but development in industrial fields such as cosmetics or medicines is insufficient.

Patent Application Publication No. 10-0945682

The present invention is to provide a composition for moisturizing the skin as a composition comprising deep sea water having a novel use for the skin.

In order to achieve the above object, an embodiment of the present invention provides a skin moisturizing composition comprising deep sea water.

The composition according to the present invention is excellent in the skin moisturizing effect by containing the deep sea water rich in minerals. In addition, the composition according to the present invention is excellent in promoting epidermal differentiation by increasing the main skin moisturizing factor involved in the maintenance of moisture of keratinocytes can prevent or improve skin dryness.

As a result, the composition according to the present invention may be variously used in the food, cosmetics, and medicine fields.

1 is a graph showing the change of the moisturizing factor for the concentration of deep sea water according to an embodiment of the present invention.
Figure 2 is a graph showing the epidermal differentiation promoting ability of deep sea water according to an embodiment of the present invention.
3 is a graph showing the change of the moisturizing factor for the content of manganese contained in the deep sea water according to an embodiment of the present invention.
Figure 4 is a graph showing the change in the moisturizing factor for the content ratio of manganese, calcium and potassium contained in the deep sea water according to an embodiment of the present invention.

As used herein, the term "sea deep water" means seawater existing at a depth of 200 m or less from the surface of the sea, and is the broadest concept of deep water obtained from the ocean regardless of the region.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

One embodiment of the present invention provides a skin moisturizing composition comprising deep sea water.

Surface water acts as a photosynthesis, so nitrogen, phosphorus, and nitric acid in the water are consumed, whereas deep ocean water is located in a deep region below 200m, so sunlight does not reach photosynthesis, and phytoplankton consume nutrients. There is no Therefore, it is rich in inorganic nutrients such as nitrogen, phosphorus, and nitric acid consumed by photosynthesis, and since it does not reach sunlight, there is no organic matter or general bacterial contamination, and there is little marine bacteria, suspensions, and adherent organisms.

The deep seawater according to an embodiment of the present invention may be a deep seawater rising from the seabed depth of 605m in a clean sea located in front of Goseong-gun, Gangwon-do, the northernmost part of the east coast of Korea. It is excellent in stability and contains abundant minerals.

Table 1 below shows the results of comparing the deep sea water quality with the deep sea water of Kochi Prefecture and the deep sea water of Hawaii, USA. It is listed with the surface water analysis for accurate comparison.

division Goseong, Korea
(Depth / surface) Kochi Prefecture, Japan
(Depth / surface) United States Hawaii
(Depth / surface) Water temperature 0.55 / 14.5 ~ 23.10 ℃ 8.1 ~ 9.8 / 16.1 ~ 24.9 ℃ 8.2 ~ 10.7 / 24.3 ~ 28.0 ℃ Hydrogen ion concentration (pH) 7.81 / 8.16-8.20 7.8 ~ 7.9 / 8.1 ~ 8.3 7.45 ~ 7.64 / 8.05 ~ 8.35 salt 34.03 / 32.5 ~ 33.0% 34.3 ~ 34.4 / 33.7 ~ 34.8% 34.37-34.29 / 34.33-35.05% Nutrients nitrate 24.29 / 0.1-1.4 μM 12.1-26.0 / 0.0-5.4 μM 39.03-40.86 / 0.24-0.42 μM Ginseng 2.26 / ND-0.7 μM 1.1-2.0 / 0.0-0.5 μM 2.89-3.15 / 0.15-0.19 μM Silicate 47.86 / 15.3-28.9 μM 33.9 ~ 56.8 / 1.6 ~ 10.1 μM 74.56 to 79.20 / 2.64 to 3.59 μM Remarks Intake depth 605m Intake depth 320m Intake depth 600m

As a result of analyzing the water temperature, pH, salinity and nutrients of deep water according to each region, the deep sea water in Goseong, Korea has a lower temperature than other deep sea water. In addition, the hydrogen ion concentration and salinity were similar to those of deep seawater in Kochi Prefecture, while the salt content of nitrate, ginseng salt and silicate salt was high.

In addition, Table 2 below is a result of comparing and analyzing the components contained in the high-performance deep sea water located at a depth of 200m or more from the viewpoint of low temperature and stability with the deep sea water of Japan.

element Goseong Deep Water Japan deep sea water Comparison of Contents of High Sea Water and Japanese Sea Water (mg / l) magnesium 1270 1320 1x calcium 367 414 1x potassium 357 410 1x salt 11033 10767 1x manganese 1.9 0.001 170 times zinc 0.68 0.009 75 times iron 0.18 0.23 8 times Copper 0.01 or less 0.0004 25 times nickel 0.01 or less 0.0004 25 times cobalt 0.01 or less 0.0002 20 times

As a result of comparing the composition of the deep sea water with the deep sea water in Japan, the deep sea water has higher mineral contents such as manganese, zinc, copper, and iron than the deep sea water of Japan due to the obvious low temperature. In addition, it can be seen that it contains various minerals such as zinc, manganese, and selenium.

Deep sea water included as an embodiment of the present invention is rich in various kinds of minerals have excellent moisturizing enhancer ability to the skin.

As an embodiment of the present invention, the high-performance deep sea water has an excellent mineral balance because the essential trace elements and various minerals are balanced and richly contained according to the analysis results.

Thus, the composition according to an embodiment of the present invention contains a high-quality deep sea water, the manganese (Mn) of the mineral main component based on the total weight of the deep sea water, 0.001% by weight or more, more specifically 0.01% by weight or more less than 10% by weight It may contain.

Manganese not only improves skin damage, but can also increase the gene expression of other skin moisturizers by being included in specific ratios with other key minerals. The skin moisturizing factor is selected from the group consisting of filaggrin (filaggrin), caspase-14, bleomycin hydrolase, and hyaluronan synthase-2. Examples of species or more can be given.

The composition according to an embodiment of the present invention includes a high-quality deep sea water, the content ratio (w / v) of manganese (Mn), calcium (Ca) and potassium (K) in the mineral main components (2/4: 0.5 to 1.5) : 0.5 to 1.5, more specifically 2.5 to 3.5: 1 to 1.5: 1 to 1.5 or 3: 1: 1 can be provided a composition. When manganese is used in the deep sea water containing calcium and potassium in the same ratio to the skin, the skin is able to maintain an ideal water retention condition is improved moisturizing power.

In addition, the composition according to an embodiment of the present invention contains a high-quality deep sea water, the content ratio (w / v) of magnesium (Mg), calcium (Ca) and potassium (K) of the main minerals of the mineral (2/4: 0.5) ˜1.5: 0.5 to 1.5, more specifically 2.5 to 3.5: 1 to 1.5: 1 to 1.5 or 3: 1: 1. This composition ratio can provide a balanced ratio of minerals to the human body, including the skin.

Therefore, the composition according to one embodiment of the present invention can promote skin moisturizing factor gene expression, and has excellent epidermal differentiation promoting ability.

As another embodiment, the present invention provides a food, cosmetic, or pharmaceutical composition containing deep sea water. In this case, the deep sea water may be manufactured within a specific hardness range and used in various fields such as food, cosmetics, or medicine.

For example, the deep sea water can be prepared as a deep sea water to be prepared by making the mineral water with a hardness of 80 mg / L ~ 1,200 mg / L, and made with concentrated water with a hardness of 12,000 mg / L, etc. Can be used for pickles, mineral salts, etc. Manufactured with mineral demineralized water with hardness less than 6,000 mg / L, it can be used for alcoholic beverages, food and beverage, wet tissues, cosmetics, etc. It can be used with tofu, cosmetics, medicines, bath products, etc. with mineral concentrated water with hardness of 120,000 mg / L or more. Alternatively, the deep sea water itself having a hardness of about 6,400 mg / L may be used for sea baths, baths, nurseries, cosmetics, foods, or the like, and may be used for food, traditional foods, etc. by manufacturing deep sea water as a salt or salt.

Hereinafter, the present invention will be described through the following examples and experimental examples. Examples and Experimental Examples are intended to describe the present invention in more detail, but the scope of the present invention is not limited to the scope of the following examples.

In addition, it is obvious that any person skilled in the art can make various modifications and imitations within the scope of the appended claims without departing from the scope of the technical idea of the present invention.

Experimental Example 1 Evaluation of Moisturizing Factor Increasing Ability According to Concentration of Deep Sea Water

In order to measure the expression promoting effect of the moisturizing factor according to the concentration of the deep sea water contained in the composition according to an embodiment of the present invention, the following experiment was carried out.

Keratinocyte growth media (KGM) containing keratinocyte growth media (HGM) containing human epidermal growth factor (hEGF, insulin, bovine pituitary extract, hydrocortisone, epinephrine, transferin, and gentamicin / amphotericin B) After passage for a certain time in the medium) cells of two passages were used. Incubate keratinocytes of two passages in a 60 mm Petri dish up to 70-80%, then treat the substance (ocean depth) by concentration (0%, 0.01%, 0.1%, 1%) Discarded, the new material and the culture was exchanged for 2 days and incubated for 4 days. The cultured cells were extracted with RNA of keratinocytes using Trizol, and cDNA synthesis was performed using the SuperScript reverse transcriptase III kit. Real-time PCR for gene comparison includes 2X TaqMan universal PCR mixture (10 μl), 20X TaqMan expression assay mix (1 μl), cDNA (50 ng) and primers (BH: Hs00166071_m1 *, Filaggrin: Hs00856927_g1 *, calpain-1 : Hs00559804_m1 *, Caspase-14: Hs00201637_m1 *, Transglutaminase-1: Hs01070310_m1 * .HAS-2; Hs00193435_m1 *) were performed using 7500 Fast Real-Time PCR. Deep-water BH (bleomycin hydrolase), Filaggrin (filagrin), caspase-14 (caspase-14), calpain-1 (calpain-1), TG-1 (transglutaminase-1), Genetic result analysis for HAS-2 (hyaluronan synthase-2) is shown in Figure 1 by quantifying by comparison with the untreated group.

As shown in FIG. 1, the expression of the skin moisturizing factor gene increases as the concentration of the deep ocean water according to an embodiment of the present invention increases. In particular, when the concentration of the deep ocean water is 1%, the expression of the moisturizing factor gene is steepest. Increased. Among the moisturizing factors, caspase-14 was the most effective in increasing the expression according to the deep ocean water.

This means that the composition according to the embodiment of the present invention can provide a skin moisturizing effect by promoting the gene expression of the skin moisturizing factor by including the deep sea water.

Experimental Example 2 Evaluation of Epidermal Differentiation Promoting Capacity of Deep Sea Water

In order to measure the epidermal differentiation promoting ability according to the concentration of the deep sea water contained in the composition according to an embodiment of the present invention, the following experiment was performed.

After culturing keratinocytes of 2 passages in 70mm Petri dish up to 70-80%, the material (ocean depth) was treated by concentration (0%, 0.01%, 0.1%, 1%) and then cultured after 2 days of culture. Discarded, the new material and the culture was exchanged for 2 days and incubated for 4 days. The cultured cells were extracted with RNA of keratinocytes using Trizol, and cDNA synthesis was performed using the SuperScript reverse transcriptase III kit. Real-time PCR for gene comparison includes 2X TaqMan universal PCR mixture (10 μl), 20X TaqMan expression assay mix (1 μl), cDNA (50 ng) and primers (Keratin 1; Hs00196158_m1, Keratin 10; Hs00166289_m1, Loricrin (LOR) Hs01894962_s1 *) was performed using 7500 Fast Real-Time PCR. Genetic results analysis for Keratin 1, Keratin 10, LOR (Loricrin) of deep sea water is shown in Figure 2 by quantifying through the non-treated group and relative comparison.

As shown in FIG. 2, the expression of Keratin-1, Keratin-10 and Loricrin, genes involved in skin keratinogenesis, increased with increasing concentration of deep seawater according to an embodiment of the present invention. At 1% concentration, the expression of each gene increased most.

This means that the composition including the deep ocean water according to one embodiment of the present invention may provide a skin moisturizing effect by increasing the expression of genes involved in skin keratinization and promoting epidermal differentiation.

Experimental Example 3 Evaluation of Moisturizing Factor Increasing Ability According to Containing Components (Mn) of Deep Sea Water

In order to measure the effect of moisturizing factor expression according to the concentration of manganese (Mn) which is the main component contained in the deep sea water according to an embodiment of the present invention was carried out the following experiment.

Two-stage keratinocytes were cultured in 60 mm Petri dishes up to 70-80%, followed by two-day incubation of Mn, one of the main components of the material (ocean depth water), by concentration (0mM, 0.1mM, 1mM, 10mM). After the culture was discarded, the new material and the culture was exchanged and treated for 2 days and incubated for a total of 4 days. The cultured cells were extracted with RNA of keratinocytes using Trizol, and cDNA synthesis was performed using SuperScript reverse transcriptase III kit. Real-time PCR for gene comparison includes 2X TaqMan universal PCR mixture (10 μl), 20X TaqMan expression assay mix (1 μl), cDNA (50 ng) and primers (BH: Hs00166071_m1 *, Filaggrin: Hs00856927_g1 *, Caspase-14 : 7500 Fast Real-Time PCR using Hs00201637_m1 *). Gene result analysis for Filaggrin (filagrin), caspase-14 (caspase-14) and BH (bleomycin hydrolase) of Mn (manganese) is shown in Figure 3 by quantifying the relative group and relative.

As shown in FIG. 3, Filaggrin (filagrin), caspase-14 (caspase-14), and BH (bleomycin) are increased as the concentration of manganese (Mn) contained in the deep seawater according to one embodiment of the present invention increases. Hydrolase) expression was increased, especially filaggrin expression was the most increased. In addition, bleomycin hydrolase showed the highest expression level when the concentration of manganese was 10 mM.

This means that the composition according to an embodiment of the present invention can provide a skin moisturizing effect by increasing the expression of the skin moisturizing factor gene by including deep sea water.

Experimental Example 4 Evaluation of Moisturizing Factor Increasing Ability According to Containing Components and Component Ratios of Deep Sea Water

In order to measure the effect of moisturizing factor expression according to the concentration of manganese (Mn), calcium (Ca ^{2 +} ) and potassium (K) which are the main components contained in the deep sea water according to an embodiment of the present invention Was carried out.

2, the composition ratio of major components which are the total concentration of Mn, Ca ^{2 +,} K of keratinocytes the post material (deep sea water), cultured in 60mm Petri dishes up to 70% to 80% of the passage such that 10mM 1: 1; 1, 2; 1: 1, 3; 1: 1 and then treated with keratinocytes and cultured for 2 days, then discarded the medicament, exchanged a new material and culture solution, and treated for 2 days and cultured for a total of 4 days. The cultured cells were extracted with keratinocytes RNA using Trizol, and cDNA synthesis was performed using the SuperScript reverse transcriptase III kit. Real-time PCR for gene comparison includes 2X TaqMan universal PCR mixture (10 μl), 20X TaqMan expression assay mix (1 μl), cDNA (50 ng) and primers (BH: Hs00166071_m1 *, Filaggrin: Hs00856927_g1 *, Caspase-14 : 7500 Fast Real-Time PCR using Hs00201637_m1 *). Gene results analysis for Filaggin (filagrin), caspase-14 (caspase-14) and BH (bleomycin hydrolase) according to the composition ratio is shown in Figure 4 by quantifying the relative treatment and relative.

As shown in Figure 4, the ratio of Mn, Ca ^{2 +} , K contained in the deep sea water according to an embodiment of the present invention 2: 1: 1, 3: 1: 1 days than when 1: 1: 1 Filaggrin, caspase-14 and caspase-14 and BH (bleomycin hydrolase) increased more, especially at 3: 1: 1. It was.

This means that the content of manganese, calcium and potassium affects the expression of the skin moisturizing factor gene, and the ratio of manganese is an important factor for the effect of deep ocean water.

Claims (9)

Including deep ocean water,
The deep sea water is a composition for moisturizing the skin containing manganese, calcium and potassium in a content ratio (w / v) of 2 to 4: 0.5 to 1.5: 0.5 to 1.5. The skin moisturizing composition according to claim 1, wherein the deep sea water contains manganese in an amount of 0.01 wt% or more and less than 10 wt% with respect to the total weight of the deep sea water. The composition of claim 1, wherein the deep ocean water contains magnesium, calcium, and potassium in a content ratio (w / v) of 2-4: 0.5-1.5: 0.5-1.5. The skin moisturizing composition according to claim 1, wherein the deep sea water has an epidermal differentiation promoting ability. The skin moisturizing composition according to claim 1, wherein the deep sea water increases the skin moisturizing factor. 6. The method of claim 5, wherein the skin moisturizing factor is filaggrin, caspase-14, bleomycin hydrolase, and hyaluronan synthase-2. Skin moisturizing composition, characterized in that at least one selected from the group consisting of. The composition for moisturizing skin according to any one of claims 1 to 6, wherein the composition is a cosmetic composition. The skin moisturizing composition according to any one of claims 1 to 6, wherein the composition is a food composition. delete

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