TH64202B - L-amino acid producing bacteria and methods of L-amino acid production - Google Patents

Abstract

DC60 (14/03/50) L-amino acids are produced by culturing bacteria of the genotropin family. Enterobacteriaceae, which are capable of producing L-amino acids and are modified so that the expression of the nhaA gene, the nhaB gene, the nhaR gene, the chaA gene, the mdfA gene or its combination, is accentuated.

Claims (7)

Disclaimer (all) which will not appear on the advertisement page. : ------ 16/10/2017 ------ (OCR) Page 1 of 3 pages Disclaimer 1. Methods for L-Amino acid production including Bacteria production L-amino acid in the medium to produce and accumulate L-amino acid in the bacterial medium or cell and Collection of L-amino acids from that medium or cell, where Such bacteria belong to the family. Enterobacteriaceae, and was modified so that the Na + / H + antiportter function was enhanced. By enhancing the expression of genes selected from the nhaA gene, nhaB gene, nhaR gene, chaA gene, mdfA gene, and their combinations by increasing the number of repetitions of the body genes. One or more, or by modifying the regulation of one or more genes. And where L-amino acids were selected from the group containing L-Lysine, L-Arginine, L-Stidine, and L-Threonine 1. And Na + / H + Antiport. 1. And has the function of Na + / H + Antiport. 1. And has Na + / H + Antiport function 1., and Na + / H + Antiport 1. And Na + / H + Antiport function. 1. Under strict conditions, and where DNA gluten transcribes functional proteins Na + / H + Antiportters 1. Under strict conditions and where DNA gluten decodes the acting proteins Functional Na + / H + Antiportor 1. Under strict conditions and where DNA decodes functional proteins Na + / H + Antiport 1 1., under strict conditions, and Where the DNA decodes the functional protein Na + / H + Antiport 1 1., under strict conditions and whereby the DNA decodes the functional protein Na + / H +. Antiporter 1 2. Method according to any of the Claims 1 to 11, whereby the bacteria belong to the genus Escherichia or Pantoea 1. 3. Method according to any one of the Claims. Verses 1 to 11, where the aforementioned bacteria is Escherichia coli ------------ Revised 4 August 2017 1. Methods for L-Amino Acid Production. Bacteria production L-amino acid in the medium to produce and accumulate L-amino acid in the bacterial medium or cell and Collection of L-Amino Acids from Intermediates Or that cell, where such bacteria belong to the family Enterobacteriaceae, and was modified so that the Na + / H + antiportter function was enhanced. By enhancing the expression of genes selected from the nhaA genes, nhaB genes, nhaR genes, chaA genes, mdfA genes, and their combinations by increasing the number of repetitions of the gene. One or more, or by altering the arrangement of one or more gene expression regulators and where L-amino acids are Was selected from a group containing L-lysine, L-arginine, L-histidine, and L-threonine ---------- 1. Bacteria that produce L-Amino acids of the family Enterobacteriaceae, where the bacteria mentioned It has been modified so that the Na + / H + antiport function is enhanced. 2. Bacteria according to claim 1, where the Na + / H + antiportter function mentioned is performed. To be distinguished by modifying the mentioned bacteria to allow the expression of the selected gene from the It consists of the nhaA gene, the nhaB gene, the nhaR gene, the chaA gene, the mdfA gene, or its combination is dominated. 3. Bacteria according to claim 2, where the aforementioned expression is enhanced by the addition of The number of repetitions of one or more genes or by modifying the expression regulator arrangement. 1. and (B) proteins that are composed of amino acid sequences including substitution, deduction, insertion, or addition of one or more amino acids in the Correspondence: 2, and function Na + / H + antiportor 1.and (D) protein composed of amino acid sequencing including substitution, deduction, insertion or deformation. The addition of one or more amino acids in the collation: 4, and the function Na + / H + antiportor 1.and (F) protein composed of amino acid sequences. Where this includes the substitution, deduction, insertion or addition of one or more amino acids in the collation: 6, and acting Na + / H + antiport 1; And (H) proteins containing amino acid sequences including substitutions, deductions, insertions or additions of one or more amino acids in the collation: 24, and is performed. Duty Na + / H + Antiporter 1 .; And (J) proteins containing amino acid sequences including substitution, deduction, insertion or addition of one or more amino acids in the collation: 26, and are performed. Function Na + / H + Antiportor 1. or a test where it is prepared from the nucleotide arrangement under strict conditions, and where the DNA in question decodes the functional protein. It performs the function Na + / H + Antiport 1 1. or a test that is prepared from the nucleotide arrangement under strict conditions, and where the DNA denotes the protein that It performs the function Na + / H + Antiport 1 1. or a test that is prepared from the nucleotide arrangement under strict conditions, and where the said DNA To decode the functional protein Na + / H + Antiport 1 1., or a test that was prepared from the nucleotide arrangement under strict conditions, and Where the DNA referred to decipher the functional protein Na + / H + Antiport 1 1., or the assay that was prepared from the nucleotide arrangement within Under strict conditions, and The DNA mentioned decoding the functional protein has the function Na + / H + Antiport 1. 4. Bacteria according to any of Claims 1 through 13 where L-Amino Acids are Choose from a group containing L-Lysine, L-Arginine, L-Histidine, L-Isoleucine, L-Valine, L-Lucine, L. L-threonine, L-phenylalanine, L-tyrosine, L-tryptophan, L-cysteine, L-glutami acid. A, and a combination of it 1 5. Bacteria according to any one of Claims 1 to 14 where the mentioned bacteria belong to the genus Escherichia or Pantoea 1. 6. Method for L-Amino Acid Production Composed of Bacterial Cultures described. Above in the medium to produce and accumulate L-amino acid in the bacterial medium or cell and the collection of L-amino acid from that medium or cell 1 7. Method for claim 16 whereby L-Amino Acids are selected from the group that Contains L-Lysine, L-Arginine, L-Histidine, L-Isoleucine, L-Valine, L-Lucine, L-Tri. Onene, L-phenylalanine, L-tyrosine, L-tryptophan, L-cysteine, L-glutamic acid, and Share of it