TH64200B - L-amino acid producing bacteria And methods for producing L-amino acids - Google Patents

Abstract

DC60 (27/01/49) The coryneform bacteria of the present invention are capable of producing L-amino acids and by facilitating the acetyl-CoA hydrolase function. The bacteria are used to produce L-amino acids generated by the biosynthetic pathway using pyruvic acid as an intermediate, such as L-glutamic acid, L-arginine, L-glutamine, L-proline, L-valine, and L-lysine. The coryneform bacteria of the present invention are capable of producing L-amino acids and by facilitating the acetyl-CoA hydrolase function. The bacteria are used to produce L-amino acids generated by the biosynthetic pathway using pyruvic acid as an intermediate, such as L-glutamic acid, L-arginine, L-glutamine, L-proline, L-valine, and L-lysine.

Claims (9)

1. Corineform bacteria capable of producing L-amino acids, where The mentioned neforms are modified to provide acetyl-CoA action. Hydrolases are reduced, and where the L-amino acids mentioned are one or more amino acids that are formed by the Biosynthetic using pyruvic acid as an intermediate 2. Corineform bacteria in accordance with Claim 1, where the function of the aforementioned acetyl-CoA isrolase is attenuated by the introduction of the mutant into the transcription region or the expression control region. of the chromosome acetyl-CoA hydrolase 3. Bacteria corine form according to claim 1, where the function of the aforementioned acetyl-CoA hydrolase is attenuated by cleavage of the acetyl-CoA high chromosome gene. Droless 4. 1. , where one or more amino acids in the mentioned proteins are substituted, subtracted, added or added, and where the mentioned proteins perform the function of acetyl- CoA Hydrolase 5. 1. or a tester prepared from polynew The cleotides mentioned, and decode acetyl-CoA hydrolases functional proteins. 6. Corine form bacteria according to one of claims 1 to 5, where The neforms mentioned were further modified to provide increased functional glutamic acid dehydrogenase. 7. Corine bacteria form pursuant to one of claims 1 to 6, where L-aminic acid The nouns mentioned are one or more L-amino acids selected from a group consisting of L-glutamic acid, L-arginine, L-glutamine, A. L-proline, L-valine, L-alanine and L-lysine 8. Methods for the production of L-amino-containing acids: Cultivation of corineform bacteria according to one of claims 1 to 7 in a medium, and collection of L-A acid. mino from the medium, and where the L-amino acids mentioned are acidic One or more L-aminos that are produced by biosynthetic pathways using pyruvic acid. as an intermediary 9. Method according to claim 8, where the L-amino acids mentioned are one or more L-amino acids selected from the L-glutamic acid group, L-arginine, L-glutamine, L-proline, L-valine, L-alanine and L-lysine.