TH61657B - Yeast produced - glutamyl cysteine - Google Patents

Abstract

DC60 (31/01/46) Yeast containing a glutathione syntase mutant with at least one or both of the mutants selected from a group consisting of mutants that replace the threonine sequence at position 47 with the isoleucine sequence and mutants that replace the glycine sequence at position 387 with the aspartic acid sequence, and produce gamma-glutamylcysteine. The yeast was cultured under optimal conditions, and the resulting culture or the resulting fractional product, which was heat-treated, was incorporated into food or beverage raw materials and processed into food or beverage formulations. Yeast containing a glutathione syntase mutant with at least one or both of the mutants selected from a group consisting of mutants that replace the threonine sequence at position 47 with the isoleucine sequence and mutants that replace the glycine sequence at position 387 with the aspartic acid sequence, and produce gamma-glutamylcysteine. Yeast is cultured under optimal conditions; the resulting culture or isolated product, after heat treatment, is then mixed with raw materials, food, or beverages, and the mixture is processed into food or beverage form.

Claims (10)

1. Yeast containing glutathionechin, which includes mutations replacing the threonine sequence at position 47 with the isoleucine sequence and mutations replacing the glycine sequence at position 387 with the aspartic acid sequence, and which produces gamma-glutamylcysteine. 2. Yeast pursuant to claim 1, a tetratete mutant containing at least one or both of the selected mutants from a group where the yeast has a further mutant that replaces the proline compound at position 54 with the leucine compound. 3. Yeast under claim 2, where the glutathione syntase mutant has a mutation replacing the threonine compound at position 47 with the isoleucine compound, and a mutation replacing the proline compound at position 54 with the leucine compound. 4. Yeast under claim 2, where the glutathione syntase mutant has a mutation replacing the glycine sequence at position 387 with the aspartic acid sequence, and a mutation replacing the proline sequence at position 54 with the leucine sequence. 5. Yeast that conforms to one of the claims 1 through 4 in which the mutagenic glutathione syntase has an amino acid sequence of the following configuration: 2. 6. Yeast according to one of the claims 1 through 5, which is of the Saccharomyces genus. 7. Food or beverages including cultures obtained by cultivating yeast according to any of the claims 1 through 6 under suitable conditions, or fractional products of the aforementioned cultures containing gamma-glutamylcysteine, or cultures or fractional products in which cysteine is produced by heat treatment. 8. Food or beverages under claim number 7 where the food or beverage is an alcoholic beverage, bread, or fermented food flavoring. 9. Yeast extracts produced using cultures obtained by cultivating yeast according to one of the claims 1 through 6 under suitable conditions. 10. Methods of producing food or beverages containing gamma-glutamylcysteine or cysteine, which include the cultivation of yeast according to one of the provisions of Patents 1 through 6 under suitable conditions; mixing of the culture or fractional product thereof; or the cultivation or fractional product thereof being heat-treated with the food or beverage raw materials; and the process of combining the mixture into food or beverages.