JPH09154563A - Soybean vinegar - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain soybean vinegar with strong sweetness and mild acidity, useful as vinegar singly or a mixture with other seasoning(s) such as PONZU (juice pressed from fitter orange), by protease treatment of Bacillus natto-treated soybean or a processed food thereof followed by conducting an acetic acid fermentation by addition of seed vinegar or ethanol. SOLUTION: Bacillus natto-treated soybean or a processed food thereof (e.g. natto) is subjected to protease treatment, the resultant system is then incorporated with seed vinegar, glucose, inorganic salt(s), water, and other ingredient(s) commonly used in acetic acid fermentation, in the presence of ethanol, followed by inoculation of acetic acid bacteria to carry out an acetic acid fermentation process, thus obtaining the objective new vinegar 2-10 wt.times or higher in amino acid content as compared to vinegar on the market, good in flavor and well-balanced in the content of essential amino acids.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to vinegar made from soybeans and a method for producing the same. The soybean vinegar according to the present invention is a novel vinegar with a strong umami and a sour acidity, which is conventionally unknown, and can be used alone as a vinegar, as well as mixed with other seasonings and the like for various cooking and food production. Can be widely used.

[0002]

2. Description of the Related Art Vinegar, especially brewed vinegar, is produced from carbohydrates such as grains, fruits, alcoholic beverages, and sake lees as raw materials by fermenting acetic acid with ethanol ("Chemical Dictionary 4", Kyoritsu Shuppan (Showa 46- 2-5) p. 829). in this way,
Conventionally, the current state of the art is that carbohydrates have been exclusively used as raw materials for vinegar.

[0003]

Vinegar is not only useful as a seasoning when used alone or in combination, but also in recent years, its importance as a health food has been increasing, and demand is expected to increase. Have been. With the diversification of consumer preferences, the production of a new type of vinegar is widely expected in the art.

[0004]

SUMMARY OF THE INVENTION The present invention has been made for the purpose of responding to the needs of the industry described above, and has set a technical problem of producing a completely new type of vinegar which has hitherto been unknown. As a result of examination from various fields for
Recognizing the need for a change in concept, he first focused on the importance of using raw materials that had never been used before to create a completely new type of vinegar.

[0005] In view of the fact that the conventional raw material for producing vinegar is mainly carbohydrates, attention is first paid to natto made from soybeans rich in protein and fat, which have completely different properties from carbohydrates and are not related to vinegar. did. And as a result of earnestly studying the conversion of natto to vinegar, after natto was treated with protease, seed vinegar and ethanol were added to it and acetic acid fermentation was performed using acetic acid bacteria.
Not only did they discover that vinegar could be produced, but they also found that the resulting vinegar was a new type of vinegar with a strong umami and a mild acidity.

In addition to natto, not only natto but also processed products of natto bacteria such as kinako, fatty soybeans and other processed soybeans can be sufficiently used as vinegar raw materials, and useful vinegar of different types can be obtained depending on the raw materials used. New facts have been discovered.

The present invention has been finally completed as a result of further studies based on these new findings. Hereinafter, the present invention will be described in detail.

[0008] As a raw material to be used, a soybean or a processed soybean product treated with Bacillus natto is widely used. Natto is a typical example of a natto treated product of soybeans, and various types of natto can be widely used. In addition, the processed soybeans may be treated with or without heat treatment, such as steaming, with or without Bacillus natt.
o In addition to Bacillus subtilis IFO 13169, etc., those obtained by inoculating and culturing and treating by inoculation with commercially available natto, or by using commercially available Bacillus subtilis natto (similarly, soybeans, soybeans) Fermented products) can also be widely used as raw materials.

[0009] Processed soybeans include defatted soybeans, okara,
Kinako, soy flour (defatted soy flour, full fat soy flour, low fat soy flour, lecithin-added soy flour, enzyme-active soy flour, tofu flour, etc.), soy grits, and various other soy-derived products can all be used.

The natto treated product thus prepared is
Hydrolyze using protease. This protease treatment may be performed by adding a protease directly to the processed product of Bacillus natto, or may be subjected to hydrolysis, crushing or pulverization, followed by addition of seed vinegar and protease treatment. The protease treatment is carried out while appropriately employing the necessary treatment.

Then, in the presence of ethanol, seed vinegar, glucose, inorganic salts, water, and other components commonly used for acetic acid fermentation were added to a predetermined amount to obtain acetic acid bacteria (Acetobacter aceti IF
Other than Acetobacter spp. Such as O 3281, commercially available seed vinegar separated and cultivated or commercially available acetic acid bacterium can be appropriately used) are transplanted, and acetic acid fermentation is performed according to a conventional method. After the fermentation, it is filtered and aged according to a conventional method to obtain a vinegar product.

The acetic acid fermentation can be any of aeration fermentation (deep fermentation) as well as stationary fermentation, and a fast vinegar method using a fermentation tower can also be used. In addition, in order to carry out acetic acid fermentation, the presence of ethanol is necessary.To that end, ethanol is directly added to mash, and if necessary, sugar such as glucose is added, and yeast is added to the mash. Alcohol fermentation may be performed to create conditions in the presence of ethanol. As the yeast, any yeast can be used as long as it can produce ethanol. For example, Saccharomyces cerevisiae IFO 1010 and other genus Saccharomyces, as well as commercially available baker's yeast, Association Nos. 7 and 9, and the like. Sake yeast, beer yeast, and the like can be used as appropriate.

Soybean vinegar thus produced (natto vinegar)
Is a novel food that has never been known before, and has a good flavor without the peculiar smell of natto, and has a mild acidity and a strong umami.
The amino acid content is high and the balance of essential amino acids is good,
It is also valuable as a health food. Its use is excellent not only as a seasoning on its own, but also as vinegar for pots and ponzu mixed with soy sauce, or as vinegar for pickling rakkyo with added sugar and salt. Hereinafter, examples of the present invention will be described.

[0014]

Example 1 25 g of natto was added to 100 ml of water, crushed with a mixer, and transferred to a 1 L Soloban ball-shaped flask (Fernbach flask). 10 ml of seed vinegar, protease M "Amano" (manufactured by Amano Pharmaceutical Co., Ltd.) 0.4
g and water (400 ml) were added and hydrolyzed at 40 ° C. for 2 days. Next, 60 g of glucose was added, and yeast (Saccharomy) was added.
(ces cerevisiae) culture solution (10 ml) was added and alcohol fermentation was performed for 3 to 4 days. To this, 100 ml of seed vinegar was added, acetic acid bacteria were transplanted, and the mixture was allowed to stand and ferment at 40 ° C. for 10 days to obtain vinegar having an acetic acid concentration of 5.16%.

[0015]

Example 2 30 ml of natto was added to 100 ml of water, crushed with a mixer, and transferred to a 1 L Soloban ball flask.
Add 40 ml of seed vinegar, protease M "Amano" 0.4
g and 360 ml of water were added and hydrolyzed at 40 ° C. for 2 days. Next, acetic acid bacteria were transplanted by adding 30 ml of ethanol and 100 ml of seed vinegar, and allowed to stand still fermentation at 40 ° C. for 10 days to obtain vinegar having an acetic acid concentration of 5.70%.

[0016]

Example 3 Skim Soybean (Nikko Oil Co., Ltd.) 25
g was steamed and sprayed with a natto (Baciillus natto) culture and placed at 30 ° C. for 2 days to produce a natto analog. This was transferred to a 1 L Soloban gem flask, and 40 ml of seed vinegar was added.
Protease M "Amano" 0.4 g, ethanol 16 m
1 and 430 ml of water were added and hydrolyzed for 2 days. next,
Seed vinegar 100ml, ethanol 20ml, glucose 12
g was added, acetic acid bacteria were transplanted, and the mixture was fermented at 40 ° C. for 10 days to obtain vinegar having an acetic acid concentration of 7.47%.

[0017]

Example 4 120 kg of crushed natto, 88 L of seed vinegar, 2.64 kg of protease M "Amano" and 1200 L of water were added and hydrolyzed at 40 ° C. for 3 days. Add 1200L of seed vinegar to this
200 L of ethanol, 10 kg of glucose, 200 g of magnesium sulfate, 400 g of second potassium phosphate, 1400 water
L was added, acetic acid bacteria were transplanted, and fermentation was carried out for 65 days to obtain vinegar having an acetic acid concentration of 5.05%.

Water was added to the natto vinegar (acidity: 5.05%) obtained in the above example to adjust the acidity to 4.40%. The analysis results of this were as follows. Total nitrogen 155.4mg / 100ml Formal nitrogen 68.5mg / 100ml Extract 1.6g / 100ml Sugar content 4.0 (Brix)

The amino acid composition of natto vinegar and the published literature values (mg / 100 ml) of various brewed vinegars are as shown in Table 1 below.

[0020]

[Table 1]

[0021]

Industrial Applicability The present invention has succeeded in producing a new vinegar using soybeans as a raw material for the first time. It has no peculiar odor derived from natto or soybean, has good flavor, and exhibits only mild acidity. No, it has particularly strong umami, high amino acid content as described above, is 2 to 10 times or more that of commercially available vinegar, and has an excellent balance of essential amino acids. Therefore, the natto vinegar according to the present invention (soybean vinegar) can be used not only as a seasoning or a health drink like ordinary vinegar, but can also be mixed with seasonings and other ingredients such as soy sauce to be used for various purposes such as ponzu. Can also be widely used.

Claims (5)

[Claims] 1. A method for producing soybean vinegar, which comprises subjecting soybean or a processed product thereof to a natto fungus treatment with protease, adding seed vinegar in the presence of ethanol, and performing acetic acid fermentation with acetic acid bacteria. 2. A method of injecting natto into a soybean or a processed product thereof, wherein the processed product of natto bacteria is natto and / or a processed soybean product selected from defatted soybean, okara, kinako, soybean flour, and soybean grits. The method according to claim 1, wherein the method is processed. 3. The method according to claim 1, wherein the conditions are set in the presence of ethanol by adding ethanol and / or ethanol fermentation. 4. The method according to claim 1, wherein the acetic acid fermentation is static fermentation and / or aeration-stirred fermentation. 5. A soy vinegar produced by the method according to any one of claims 1 to 4.