TH40451A - A pollen-based genetic modification system using solid-culture media - Google Patents

Abstract

DC60 (13/07/41) What was revealed is a feed that supports the germination of pollen. And pipe growth Pollen while having Agrobacterium The feed contains agarose, sucrose NO3, MnSO4, H3BO3, MgSO4 and gibberellic acid. What is also disclosed is a method for change. Genetics of plants and plant species by genetic changes with Agrobacterium is a pollen-based medium. The method consists of a first plant-based pollination process, the addition of agrobacteria to a solid pollen feed. Agrobacterium is made up of one or more genes from other organisms that can be transferred to plant cells. Allowing pollen to germinate and flourish on the feed by this method Agrobacterium Binds to pollen ducts or mediates gene sequencing transfer from existing Other species of life to the germinating pollen to allow gene transfer pollen. The gene transfer pollen is placed on the tip of the grain. The female of the second plant can be fertilized by the first plant pollen. In this way, it is bred to the second plant, to obtain the second plant gene transfer seed. And making the seeds transfer genes to germinate to To get a gene transfer plant The method is particularly suitable for use with a type of pollen known as "dry female plume", which is revealed as a feed that supports the germination of pollen. And pipe growth Pollen while having Agrobacterium The feed contains agarose, sucrose No3, MnSo4, H3BO3, MgSO4 and gibberellic acid. What is also disclosed is a method for change. Genetics of plants and plant species by genetic changes with Agrobacterium is a pollen-based medium. The method consists of a first plant-based pollination process, the addition of agrobacteria to a solid pollen feed. Agrobacterium is made up of one or more genes from other organisms that can be transferred to plant cells. Allowing pollen to germinate and flourish on the feed by this method Agrobacterium Binds to pollen ducts or mediates gene sequencing transfer from existing Other species of life to the germinating pollen to allow gene transfer pollen. Injecting gene transfer pollen onto the end of agriculture. The female of the second plant can be fertilized by the first plant pollen. In this way, it is bred to the second plant, to obtain the second plant gene transfer seed. And making the seeds transfer genes to germinate to To get a gene transfer plant This method is especially suitable for use with a type of pollen called "dry female plume".

Claims (6)

1. Methods for producing plant-transcribing genes consisting of a. Obtaining first plant pollinators b. Attaching tracts of Agrobacteria to solid pollinators Agrobacteria consist of gene sequences from other organisms. At least one sequence can be taken. Can be transferred to plant cells c. Pollen is added to a type of culture medium d. Letting pollen germinate and grow on the culture medium in this way can cause gene transfer pollen e. The female seed of the second plant that can be treated Mixed by first plant pollen In this way, the second plant is inbreeding f. To obtain the gene transfer from the second plant g. The germination of the seed to obtain the gene transfer plant. 2. Method of claim 1 where the first and second crops were selected from a group consisting of tobacco, cotton and soybean plants. 3. Claim Method 1 in which the solid-pollinated feed contains 10 percent agarose, 25 percent sucrose, KNO3 0.52 mmolar MnSO4, 3.06 mmolar H3BO4 1.66 mm MgSO4. 7H2O 0.42. Mmolar and gibberellic acid A3 1.0 μmolar 4. Method of claim 1 in which those plants are dry-pollinated plants. 5. Method of claim 4 when those plants are cotton. 6. Method of claim 5 where the second crop of cotton is the cotton that has eliminated male grains. 6. Procedure for claim 1, which includes an additional step that eliminates gays. The male is given to the second plant before the pollinator is injected onto that plant.