TH3471B - Method for altering double stranded DNA - Google Patents

Abstract

This proposed invention addresses the problems related to tryptophan reactions and attenuation in a different way and provides (1) methods for obtaining the vectors of X. pressure designed For direct expression of genes from other organisms from the promoter-operator trp system (2). Obtain a vector designed for polypep expressions. tides, which are specifically cleaved from tryptophan operator-promoters and coded by standing by the gene binding of the Other organisms with bacterial genes (3) one method of expression of polypeptides of other organisms Good control, high efficiency and high productivity, no less. go beyond the related way

Claims (3)

1. A process for cutting double stranded DNA at any given point consisting of (a) converting double stranded DNA into single stranded DNA around that point (b) hybridize. With a single line area Obtained in step (a) with the supplementary primer whose length is the same as the single strand of DNA, the 5 \ 'tip of the primer is exactly Across with a nucleotide located at 5 \ 'before the mind location is cut (c) creating a second line segment that is largely eliminated from the step (a) located in the direction 3 \' from the plant. The polymer by reacting with DNA polymers with deoxynucleotides. Triphosphate phase containing Adenine, thymine, guanine (D) Remaining single strand length of DNA that extends beyond the point where it was intended to be cut. 2. Process according to claim 1, where steps (c) and (d) are mutually exclusive by reaction with DNA polymerase, which polymerizes in the direction of 5. \ '- 3 \' will be exo Nucleolytic in the direction of 3 \ '- 5 \' but will be non-exo-nuclei. Olitic in the 5 \ '- 3 \' direction 3. Clause 2 Clause 2 where the polymer is Clain Polymerase I (3 Claims, 1 Page, 13 Photos).