TH613A - Method for altering double stranded DNA - Google Patents

Abstract

The proposed invention addresses a different issue with respect to tryptophan, expression and attribute, and provides (1) a method for obtaining the carrier of EX. Expression Designed For expressing genes from other organisms directly from the trp promoter-operator system (2). Acquired the carrier designed for the polypepression Tides, which are specifically cut from the tryptophan-operator and stand-by code derived from the gene binding of the thing. (3) One method of polypeptide expression of other organisms in which Good control, high efficiency and high productivity Go beyond how they relate

Claims (3)

1. A process for cutting double stranded DNA at any given point consisting of (a) converting double stranded DNA into single stranded DNA around that point (b) hybridize. With a single line area Obtained in step (a) with the supplementary primer whose length is the same as the single strand of DNA, the 5 \ 'tip of the primer is exactly Across with a nucleotide located at 5 \ 'before the mind location is cut (c) creating a second line segment that is largely eliminated from the step (a) located in the direction 3 \' from the plant. The polymer by reacting with DNA polymers with deoxynucleotides. Triphosphate phase containing Adenine, thymine, guanine (D) Remaining single strand length of DNA that extends beyond the point where it was intended to be cut. 2. Process according to claim 1, where steps (c) and (d) are mutually exclusive by reaction with DNA polymerase, which polymerizes in the direction of 5. \ '- 3 \' will be exo Nucleolytic in the direction of 3 \ '- 5 \' but will be non-exo-nuclei. Olitic in the 5 \ '- 3 \' direction 3. Clause 2 Clause 2 where the polymer is Clain Polymerase I (3 Claims, 1 Page, 13 Photos).